CN108315374A - A kind of environment-friendly preparation method thereof of superelevation branch converted starch particle - Google Patents
A kind of environment-friendly preparation method thereof of superelevation branch converted starch particle Download PDFInfo
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- CN108315374A CN108315374A CN201810048745.3A CN201810048745A CN108315374A CN 108315374 A CN108315374 A CN 108315374A CN 201810048745 A CN201810048745 A CN 201810048745A CN 108315374 A CN108315374 A CN 108315374A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/22—Preparation of compounds containing saccharide radicals produced by the action of a beta-amylase, e.g. maltose
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
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Abstract
The present invention relates to Starch Production technical fields, particularly disclose a kind of environment-friendly preparation method thereof of superelevation branch converted starch particle.The environment-friendly preparation method thereof of the superelevation branch converted starch particle, it is characterised in that：Using starch from sweet potato as receptor, malto-oligosaccharide is donor, and enzyme hydrolysis and transglucosidation are carried out successively to starch from sweet potato using branching enzyme, beta amylase and glycosyltransferase, obtains the superelevation branch converted starch particle containing higher branch's short chain proportional numbers.Present invention process is simple, short preparation period, need not add special installation, and production cost is low, is suitable for industrialized production, and do not need any additive, safety non-pollution during producing.
Description
（One）Technical field
The present invention relates to Starch Production technical field, more particularly to the green preparation side of a kind of superelevation branch converted starch particle
Method.
（Two）Background technology
Starch is widely used in food and industrial circle as a kind of important raw material of industry, to reinforce improving each of product
Kind processing performance.Starch is also the important composition ingredient in human diet, and glucose is converted to after human consumption to provide
Energy necessary to people's vital movement.However, as what the progress of foods processing technique and starch science were studied gos deep into, people
It was found that ative starch has significant limitation in the application：Stability is poor, is easy aging；Dissolubility is poor；It is solidifying in starch
In glue, the moiety segments of starch molecule can be subjected to displacement generation VISCOUS FLOW, cause starch gel elasticity to change, hardness
Greatly, there is harsh feeling etc..Meanwhile digestion rate comparatively fast easily leads to postprandial blood sugar and increases rapidly ative starch in vivo, causes high blood
The diseases such as sugar, obesity, angiocarpy.
To solve the problems, such as these present in ative starch, research at present has using physics, chemistry, gene or enzyme process and answers
It closes modified method to be modified ative starch, so as to improve its physical and chemical performance and digestic property.Wherein, chemical modification starch has been
It is widely used in food industry, but the problems such as there are safety and environmental pollutions, the preparation side of enzyme modification starch
Method environmental protection, action condition is simple mild and has the advantages that be easy to be decomposed by human consumption.
（Three）Invention content
In order to compensate for the shortcomings of the prior art, the present invention provides a kind of short preparation period, production cost are low, safety non-pollution
The environment-friendly preparation method thereof of superelevation branch converted starch particle.
The present invention is achieved through the following technical solutions：
A kind of environment-friendly preparation method thereof of superelevation branch converted starch particle, it is characterised in that：Using starch from sweet potato as receptor, malt is low
Glycan is donor, carries out enzyme hydrolysis successively to starch from sweet potato using branching enzyme, beta amylase and glycosyltransferase and turns sugar
Glycosides acts on, and obtains the superelevation branch converted starch particle containing higher branch's short chain proportional numbers.
The present invention use combined-enzyme method modified sweet potato starch, first by branch's enzyme effect starch from sweet potato when, hydrolyze starch
Middle amylose and the alpha-1, 4-glucosidic key in amylopectin straight chain area generate cluster and some linear glucose residues, are catalyzed simultaneously
Transfer glucose residue is connected to the formation for promoting alpha-1, 6-glucosidic key on other chains, generates new branch, makes starch molecule
Branch's density increases.Then further being hydrolyzed using beta amylase makes its external chain length shorten, and finally glucoside is utilized to shift
Free glucose residue and malto-oligosaccharide are connected to other by the glucosyl group effect that turns of enzyme in the form of a-1,6 glycosidic bonds
On chain, more new short chain side branch is formd, further increases its branch's density, more close structure is formed, is contained
The superelevation branch converted starch particle of higher branch's short chain proportional numbers, improves the degree of branching of starch from sweet potato, no chemical residues.
It specifically comprises the following steps：
（1）Starch from sweet potato is configured to 5（w/v）The starch fluid of %, it is 6.5 to adjust starch fluid pH；
（2）Branching enzyme is added into starch fluid, hydrolysis is vibrated in water-bath, then enzyme deactivation activity, cooling obtains enzymolysis liquid；
（3）It is 5.2 to adjust enzymolysis liquid pH, and beta amylase is added, and hydrolysis is shaken in water-bath, then enzyme deactivation activity, and cooling obtains
Enzymolysis liquid；
（4）Malto-oligosaccharide is added into enzymolysis liquid, adjusts pH to 5.0 after mixing, adds glycosyltransferase, set
Enzymolysis is vibrated in water-bath, then enzyme deactivation activity, sample liquid is obtained after cooling；
（5）Absolute ethyl alcohol is added into sample liquid, centrifuges, gained precipitation is freeze-dried to obtain product.
The present invention more excellent technical solution be：
Step（1）In, weigh 10g starch from sweet potato, the 0.02M sodium acetate buffers that addition pH is 6.5 to farinaceous size volume
For 200mL.
Step（2）In, the branching enzyme of 308U/g is added into starch fluid, is placed in 60 DEG C of water-baths and vibrates 20-24h, then
The NaOH solution enzyme deactivation activity of 2.5mL 1mol/L is added.
Step（3）In, the beta amylase of 10u/g is added into enzymolysis liquid, is placed in 37 DEG C of water-baths and vibrates 4-6h, then adds
Enter the NaOH solution enzyme deactivation activity of 2.5mL 1mol/L.
Step（4）In, the malto-oligosaccharide for accounting for its weight ratio 5% is added into enzymolysis liquid, 9088U/ is added after mixing
The glycosyltransferase of g is placed in oscillation enzymolysis 10-12h in 55 DEG C of water-baths, the NaOH solution of 2.5mL 1mol/L is then added
Enzyme deactivation activity；Wherein, the malto-oligosaccharide by weight proportion be 20% maltotetraose, 30% maltopentaose, 40% malt
Six sugared and 10% seven sugar mixing of malt form.
Step（5）In, the absolute ethyl alcohol of its 3 times of volumes is added into sample liquid, is centrifuged under conditions of 3000r/min
10min is precipitated.
Present invention process is simple, short preparation period, need not add special installation, and production cost is low, is suitable for industrial metaplasia
Production, and do not need any additive, safety non-pollution during producing.
（Four）Description of the drawings
The present invention will be further described below with reference to the drawings.
Fig. 1 is the SEM electron microscopes of former starch from sweet potato；
Fig. 2 is the SEM electron microscopes of converted starch grain products of the present invention；
Fig. 3 is that former starch from sweet potato black cross under petrographic microscope observes image；
Fig. 4 is that converted starch particle of the present invention black cross under petrographic microscope observes image.
（Five）Specific implementation mode
Embodiment：A kind of preparation method of superelevation branch converted starch particle
Specifically include following steps：
（1）The preparation of starch from sweet potato liquid：Accurately weighing 10g starch from sweet potato samples is made into 5%（w/v）Starch fluid adjusts starch solution
PH is 6.5；
（2）Branching enzyme digests：Branching enzyme (308U/g) is placed in 60 DEG C of water-bath oscillations for 24 hours, is then added 2.5mL 1mol/L's
NaOH solution makes enzyme-deactivating, cooling；
（3）Beta amylase hydrolyzes：It is 5.2 to adjust pH value of solution, beta amylase enzyme additive amount 10U/g, enzymolysis time 6h, reaction temperature
37 DEG C, the NaOH solution of 2.5mL 1mol/L, which is added, makes enzyme-deactivating, cooling；
（4）The addition of malto-oligosaccharide：Weigh 5g malto-oligosaccharide mixtures, including 20% maltotetraose, 30% maltopentaose,
40% maltose, 10% malt, seven sugar, are added in enzymolysis liquid and are uniformly mixed；
（5）Glycosyltransferase digests：It is 5.0 to adjust pH value of solution, and glycosyltransferase additive amount 9088U/g is placed in 55
DEG C water-bath oscillation enzymolysis 12h, the NaOH solution of 2.5mL 1mol/L, which is then added, makes enzyme-deactivating；
（6）It waits for that sample liquid is cooled to room temperature, the absolute ethyl alcohol of 3 times of volumes is added, the centrifugation of 10min is carried out under 3000 r/min,
Gained pellet frozen drying to obtain superelevation branch converted starch particle.
Materialization and the detection of digestion characteristics are carried out to the converted starch particle that the present embodiment obtains, after testing it is found that with original
Starch is compared, and converted starch grain dissolution degree of the invention is significantly increased to 47.65% from 9.86%, and viscosity increases
43.24%；Gelatinization peak temperature is reduced to 56.94 DEG C from 68.98 DEG C；Gelatinized corn starch is stored one week at 4 DEG C, Degree of Ageing from
58.94% is reduced significantly to 36.57%；Slowly digestible starch and resistance starch content are dramatically increased from 24.65% and 8.68% respectively
To 48.34% and 26.87%.
The converted starch particle that the present embodiment is obtained carries out application test：
（1）In Flour product, delaying aging improves mouthfeel, stability of viscidity, elasticity of Flour product etc. for addition；
（2）Applied to cosmetic industry, the additive of foundation cream can be used as；
（3）It is excellent in food processing, processing technology can be facilitated, provided for food as a kind of polyfunctional food additive
Texture, improve thickening, suspension, water conservation and the stabilizing power of starch；
（4）It is used for numerous food as functional components, digestion rate is significantly reduced, to reduce blood glucose burst size.
Claims (8)
1. a kind of environment-friendly preparation method thereof of superelevation branch converted starch particle, it is characterised in that：Using starch from sweet potato as receptor, malt
Oligosaccharide is donor, carries out enzyme hydrolysis successively to starch from sweet potato using branching enzyme, beta amylase and glycosyltransferase and turns
Glucosides acts on, and obtains the superelevation branch converted starch particle containing higher branch's short chain proportional numbers.
2. the environment-friendly preparation method thereof of superelevation branch converted starch particle according to claim 1, it is characterized in that, it specifically includes
Following steps：（1）Starch from sweet potato is configured to 5（w/v）The starch fluid of %, it is 6.5 to adjust starch fluid pH；（2）Add into starch fluid
Enter branching enzyme, hydrolysis is vibrated in water-bath, then enzyme deactivation activity, cooling obtains enzymolysis liquid；（3）It is 5.2 to adjust enzymolysis liquid pH, is added
Enter beta amylase, hydrolysis is shaken in water-bath, then enzyme deactivation activity, cooling obtains enzymolysis liquid；（4）Malt is added into enzymolysis liquid
Oligosaccharide adjusts pH to 5.0, adds glycosyltransferase after mixing, is placed in water-bath and vibrates enzymolysis, then enzyme deactivation
Activity obtains sample liquid after cooling；（5）Absolute ethyl alcohol is added into sample liquid, centrifuges, gained precipitation is freeze-dried to obtain product.
3. the environment-friendly preparation method thereof of superelevation branch converted starch particle according to claim 2, it is characterised in that：Step
（1）In, 10g starch from sweet potato is weighed, it is 200mL that 0.02M sodium acetate buffers to the farinaceous size volume that pH is 6.5, which is added,.
4. the environment-friendly preparation method thereof of superelevation branch converted starch particle according to claim 2, it is characterised in that：Step
（2）In, the branching enzyme of 308U/g is added into starch fluid, is placed in 60 DEG C of water-baths and vibrates 20-24h, 2.5mL is then added
The NaOH solution enzyme deactivation activity of 1mol/L.
5. the environment-friendly preparation method thereof of superelevation branch converted starch particle according to claim 2, it is characterised in that：Step
（3）In, the beta amylase of 10u/g is added into enzymolysis liquid, is placed in 37 DEG C of water-baths and vibrates 4-6h, 2.5mL is then added
The NaOH solution enzyme deactivation activity of 1mol/L.
6. the environment-friendly preparation method thereof of superelevation branch converted starch particle according to claim 2, it is characterised in that：Step
（4）In, the malto-oligosaccharide for accounting for its weight ratio 5% is added into enzymolysis liquid, the glucoside of 9088U/g is added after mixing
Transferase is placed in oscillation enzymolysis 10-12h in 55 DEG C of water-baths, and the NaOH solution enzyme deactivation activity of 2.5mL 1mol/L is then added.
7. the environment-friendly preparation method thereof of superelevation branch converted starch particle according to claim 2, it is characterised in that：Step
（5）In, the absolute ethyl alcohol of its 3 times of volumes is added into sample liquid, 10min is centrifuged under conditions of 3000r/min, is precipitated.
8. the environment-friendly preparation method thereof of the superelevation branch converted starch particle according to claim 2 or 6, it is characterised in that：Institute
It is 20% maltotetraose, 30% maltopentaose, 40% maltose and 10% malt that malto-oligosaccharide, which is stated, by weight proportion
Seven sugar mixing compositions.
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| CN201810048745.3A CN108315374B (en) | 2018-01-18 | 2018-01-18 | Preparation method of high-branch modified starch granules |
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| CN201810048745.3A CN108315374B (en) | 2018-01-18 | 2018-01-18 | Preparation method of high-branch modified starch granules |
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| CN108315374B CN108315374B (en) | 2021-04-27 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109988798A (en) * | 2019-04-16 | 2019-07-09 | 齐鲁工业大学 | A kind of environment-friendly preparation method thereof of rice resistant starch |
| CN110438183A (en) * | 2019-07-25 | 2019-11-12 | 哈尔滨商业大学 | Combined-enzyme method modified starch prepares the application of the method and low DE value maltodextrin of low DE value maltodextrin in food |
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| EP1369432A2 (en) * | 2002-06-06 | 2003-12-10 | Roquette Frˬres | Soluble highly branched glucose polymers and process for their preparation |
| CN101595226A (en) * | 2007-02-01 | 2009-12-02 | Cj第一制糖株式会社 | The enzymatic highly branched amylose starch and the preparation method of amylopectin cluster |
| CN101715911A (en) * | 2009-12-10 | 2010-06-02 | 上海市农业科学院 | Method for preparing digestion starch containing slow digestion starch by enzymolysis |
| CN104293865A (en) * | 2014-10-27 | 2015-01-21 | 江南大学 | Synthetic method multi-branch starch |
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| WO2000018893A1 (en) * | 1998-09-25 | 2000-04-06 | Roquette Freres | Method for preparing a mixture of starch branching enzymes extracted from algae |
| EP1369432A2 (en) * | 2002-06-06 | 2003-12-10 | Roquette Frˬres | Soluble highly branched glucose polymers and process for their preparation |
| CN1468867A (en) * | 2002-06-06 | 2004-01-21 | �ֵ����� | Soluble high branched glucose polymer and its producing process |
| CN101595226A (en) * | 2007-02-01 | 2009-12-02 | Cj第一制糖株式会社 | The enzymatic highly branched amylose starch and the preparation method of amylopectin cluster |
| CN101715911A (en) * | 2009-12-10 | 2010-06-02 | 上海市农业科学院 | Method for preparing digestion starch containing slow digestion starch by enzymolysis |
| CN104293865A (en) * | 2014-10-27 | 2015-01-21 | 江南大学 | Synthetic method multi-branch starch |
Non-Patent Citations (14)
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109988798A (en) * | 2019-04-16 | 2019-07-09 | 齐鲁工业大学 | A kind of environment-friendly preparation method thereof of rice resistant starch |
| CN110438183A (en) * | 2019-07-25 | 2019-11-12 | 哈尔滨商业大学 | Combined-enzyme method modified starch prepares the application of the method and low DE value maltodextrin of low DE value maltodextrin in food |
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