CN108308431B - Pig feed additive containing riboflavin fermentation waste liquid and preparation method thereof - Google Patents
Pig feed additive containing riboflavin fermentation waste liquid and preparation method thereof Download PDFInfo
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- CN108308431B CN108308431B CN201810430284.6A CN201810430284A CN108308431B CN 108308431 B CN108308431 B CN 108308431B CN 201810430284 A CN201810430284 A CN 201810430284A CN 108308431 B CN108308431 B CN 108308431B
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention discloses a pig feed additive containing riboflavin fermentation waste liquid, which comprises the following raw materials in parts by weight: 30-60 parts of riboflavin fermentation waste liquid, 30-50 parts of folium artemisiae argyi and 5-60 parts of marigold residues. The riboflavin fermentation waste liquid, the folium artemisiae argyi and the marigold residues in the riboflavin-containing feed additive formula provided by the invention have synergistic effect after compounding, the growth of pigs is promoted, the morbidity is reduced, the oestrus rate and the farrowing rate of sows are improved, the economic benefit of farmers is further improved, and the breeding cost is reduced. The invention also provides a preparation method of the feed additive, which is simple and easy to operate and is convenient for large-scale production.
Description
Technical Field
The invention relates to the field of feed additives, in particular to a pig feed additive containing riboflavin fermentation waste liquid and a preparation method thereof.
Background
At present, riboflavin, also known as vitamin B2, is a water-soluble vitamin necessary for human bodies, and with the development of science and technology, riboflavin has important values in clinical treatment, feed industry, food industry and cosmetic industry. At present, the international production process of riboflavin mainly comprises 4 processes: extraction method, chemical synthesis method, microbial fermentation method and semi-microbial fermentation semi-chemical synthesis method. Among them, the microbial fermentation method has low cost and little pollution, so it is increasingly favored by riboflavin manufacturers in the world.
Along with social development, the requirement of environmental protection on industrial production is higher and higher, and the problem to be solved is that how to treat fermentation waste liquid generated in the process of preparing riboflavin by a microbial fermentation method can meet the requirement of environmental protection emission. The riboflavin fermentation waste liquid contains protein, polysaccharide which is not used up in fermentation, amino acid, inorganic salt and residual strains which are not extracted completely, the fermentation waste liquid has higher treatment cost, and the residues in the waste liquid are wasted. The invention patent of publication No. CN102010104B discloses a method for treating riboflavin fermentation waste liquid, the waste liquid is concentrated by a multi-effect evaporation system, concentrated slurry is dried to obtain a large amount of solid protein, and condensate liquid is biochemically treated by anaerobic UASB + A/O + contact oxidation decoloration and then is discharged after reaching standards. However, the cost of evaporation and concentration of the waste liquid is high, and the solid protein obtained after the riboflavin fermentation waste liquid is concentrated and dried cannot be directly used as animal feed, because the waste liquid contains a large amount of mycotoxin, and the animal health is seriously harmed if the content exceeds the standard.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide the pig feed additive containing the riboflavin waste liquid, which can obviously improve the growth condition of pigs, improve the immunity of the pigs and promote the growth of the pigs.
The invention also aims to provide a preparation method of the pig feed additive containing the riboflavin waste liquid.
One of the purposes of the invention is realized by adopting the following technical scheme:
a pig feed additive containing riboflavin fermentation waste liquid comprises 30-60 parts of riboflavin fermentation waste liquid, 30-50 parts of folium Artemisiae Argyi and 5-60 parts of marigold slag.
Further, the riboflavin waste liquid is waste liquid of riboflavin residue prepared by a fermentation method.
Furthermore, the feed additive also contains 0.1 to 0.3 part of preservative, 0.5 to 1 part of sweetener and 0.5 to 1 part of mildew removing agent.
Further, the feed additive comprises the following raw materials: 50 parts of riboflavin fermentation waste liquid, 45 parts of folium artemisiae argyi, 5 parts of marigold slag, 0.2 part of preservative, 0.5 part of sweetening agent and 0.5 part of mildew removing agent.
Further, the preservative is sodium benzoate.
Further, the sweetener is saccharin sodium.
Further, the mold removing agent is aflatoxin decomposition enzyme.
The second purpose of the invention is realized by adopting the following technical scheme:
the preparation method of the pig feed additive containing the riboflavin waste liquid comprises the following steps:
(1) Pulverizing folium Artemisiae Argyi and flos Tagetis Erectae residue, adding into riboflavin fermentation waste liquid, stirring, mixing well, and fermenting;
(3) Drying the mixture fermented in the step (2) until the water content is 35-65%;
(4) And (4) adding the preservative, the mildew removing agent and the sweetening agent into the mixture dried in the step (3) and uniformly stirring to obtain the pig feed additive.
Further, the fermentation temperature in the step (2) is 40-50 ℃, and the fermentation time is 40-48h.
Compared with the prior art, the invention has the beneficial effects that: the riboflavin fermentation waste liquid, the folium artemisiae argyi and the marigold residues in the feed additive formula containing the riboflavin provided by the invention have synergistic effect after compounding, the growth of pigs is promoted, the morbidity is reduced, the oestrus rate and the farrowing rate of sows and the survival rate of piglets are improved, the cost of raw materials is further reduced by adopting the riboflavin fermentation waste liquid and the marigold residues, the economic benefit of farmers is further improved, and the breeding cost is reduced. The invention also provides a preparation method of the feed additive, which is simple and easy to operate and is convenient for large-scale production.
Detailed Description
The present invention is further described below with reference to specific embodiments, and it should be noted that, without conflict, any combination between the embodiments or technical features described below may form a new embodiment.
Example 1
A pig feed additive containing riboflavin fermentation broth is prepared from the following raw materials in parts by weight: 40 parts of riboflavin fermentation waste liquid, 35 parts of folium artemisiae argyi, 25 parts of marigold slag, 0.2 part of sodium benzoate, 0.5 part of saccharin sodium and 0.5 part of aflatoxin decomposition enzyme.
The preparation method of the feed additive comprises the following steps:
(1) Pulverizing folium Artemisiae Argyi and flos Tagetis Erectae residue, adding into riboflavin fermentation waste liquid, stirring, mixing, and fermenting at 40 deg.C for 48h;
(3) Drying the mixture fermented in the step (2) until the water content is 45%;
(4) And (4) adding sodium benzoate, saccharin sodium and aflatoxin decomposition enzyme into the dried mixture obtained in the step (3), and uniformly stirring to obtain the feed additive for pigs.
Example 2
A pig feed additive containing riboflavin fermentation broth is prepared from the following raw materials in parts by weight: 60 parts of riboflavin fermentation waste liquid, 30 parts of folium artemisiae argyi, 10 parts of marigold slag, 0.1 part of sodium benzoate, 0.5 part of saccharin sodium and 0.5 part of aflatoxin decomposition enzyme.
The preparation method of the feed additive comprises the following steps:
(1) Pulverizing folium Artemisiae Argyi and flos Tagetis Erectae residue, adding into riboflavin fermentation waste liquid, stirring, mixing, and fermenting at 45 deg.C for 45 hr;
(3) Drying the mixture fermented in the step (2) until the water content is 60%;
(4) And (4) adding sodium benzoate, sodium saccharin and aflatoxin decomposition enzyme into the mixture dried in the step (3) and uniformly stirring to obtain the feed additive for pigs.
Example 3
A pig feed additive containing riboflavin fermentation broth is prepared from the following raw materials in parts by weight: 30 parts of riboflavin fermentation waste liquid, 45 parts of folium artemisiae argyi, 25 parts of marigold slag, 0.2 part of sodium benzoate, 0.5 part of saccharin sodium and 0.5 part of aflatoxin decomposition enzyme.
The preparation method of the feed additive comprises the following steps:
(1) Pulverizing folium Artemisiae Argyi and flos Tagetis Erectae residue, adding into riboflavin fermentation waste liquid, stirring, mixing well, fermenting at 50 deg.C for 40 hr;
(3) Drying the mixture fermented in the step (2) until the water content is 35%;
(4) And (4) adding sodium benzoate, sodium saccharin and aflatoxin decomposition enzyme into the mixture dried in the step (3) and uniformly stirring to obtain the feed additive for pigs.
Comparative example 1
Comparative example 1 provides a pig feed additive, which is different from example 1 in that mugwort leaf and marigold slag are omitted, the amount of riboflavin fermentation waste liquid is adjusted to 100 parts, and the rest is the same as example 1.
Comparative example 2
Comparative example 2 provides a pig feed additive, which is different from example 1 in that riboflavin fermentation waste liquid is omitted, and the amount of mugwort leaves and marigold slag is adjusted to 100 parts, and the rest is the same as example 1.
Comparative example 3
Comparative example 3 provides a pig feed additive which is different from example 1 in that the riboflavin fermentation waste liquid is replaced with vitamin B2, and the rest is the same as example 1.
Comparative test of effects
The experimental method comprises the following steps: the method comprises the steps of selecting Du's growing three-element hybrid piglets, wherein the daily age of the piglets is less than or equal to 28 days, the weight of the piglets is less than or equal to 7.5kg for 520 heads, dividing the piglets into three groups, taking 130 heads respectively, adopting free feeding, adopting porridge feed in the first three days and dry feed in the last 7 days, respectively adding the feed additives of the embodiment 1 and the comparative examples 1-3 into the feed, wherein the dosage of the feed additives is 2 percent of the feed, and the test days are 10 days. In the experiment, except for the treatment difference specified by the experiment design, other feeding management conditions and technical measures are consistent in quality, the diarrhea condition of piglets and the mortality rate of the piglets are observed, and the daily gain is measured.
TABLE 1
It can be seen from table 1 that the average daily gain of the test group fed with the feed additive containing example 1 was higher than that of the test group fed with the feed additives containing comparative examples 1 to 3, and that the diarrhea rate and the mortality rate were lower than those of the comparative examples 1 to 3. The feed additive provided by the invention is proved to obviously promote the food intake of piglets, reduce the diarrhea rate and promote the growth by using the Chinese medicinal components of the Chinese mugwort leaves and the marigold residues in combination with the riboflavin fermentation waste liquid, when one component is omitted or replaced, the effect is still inferior to that of the combination of the three components under the condition of keeping the same dosage, and the Chinese mugwort leaves, the marigold residues and the riboflavin fermentation waste liquid have obvious gain effect by the compatibility and the synergistic cooperation.
The pigs with uniform health degree and uniform weight uniformity at the nursery transition stage are selected as experimental specimens and grouped into four groups, wherein each group has 100 pigs, and 3% of the feed additive of the embodiment 1 and the comparative examples 1 to 3 is added into the daily ration of each group. And then collecting the sick pig corresponding to the pathogenic microorganism, taking viscera and mucosa, crushing, taking supernate, and infecting experiment specimens through food and air. In order to avoid excessive economic losses, after the incubation period, 1 pig had developed in any of the three groups, and the four groups were treated immediately with the same antibiotic formulation, with the results shown in table 2.
TABLE 2
As can be seen from Table 2, compared with the feed additives provided by feeding comparative examples 1 and 3, the feed additive provided by the invention can effectively improve the body resistance and reduce the infection of the swinery to bacterial diseases, even after the infection, the disease course is short, the symptoms are light, the treatment effect is good, the influence on the growth speed of the swinery is small, the stiff pigs and the pigs falling behind due to diseases can not occur, and the economic benefit of the whole swinery is not influenced.
The day age of the growing-up binary sow is more than or equal to 220 days, the weight of the growing-up binary sow is more than or equal to 130kg, the growing-up binary sow is divided into 240 groups, each group comprises 60 sows, the sow adopts free feeding, 4 percent of the feed additive in the embodiment 1 and the comparative examples 1 to 3 is respectively added into the feed, the adult boar is used for trying for one hour every day, the experimental days are 10 days, the feed is unified, the management is unified, and the results are shown in the table 3.
TABLE 3
| Group of | Example 1 | Comparative example 1 | Comparative example 2 | Comparative example 3 |
| Number of oestrus | 56 | 40 | 42 | 47 |
| The oestrus rate% | 93％ | 67％ | 70％ | 78％ |
Table 3 shows that the addition of the feed additive of the present application to the feed is helpful for the oestrus of sows, the effect of the feed additive provided in comparative examples 1 and 3 is inferior to that of the feed additive provided in the present invention after the traditional Chinese medicine components are replaced, the oestrus rate of sows added with the feed additive of example 1 is as high as 93%, the oestrus rate of sows added with the feed additive of comparative example 1 is only 67%, 70% and 78%, and is far lower than that of example 1, and the feed additive provided in the present invention is used for remarkably improving the oestrus rate of sows by compounding the riboflavin fermentation waste liquid, the artemisia leaf and the marigold residues.
The growing binary 2-5-farrowing sows are selected, 320 pregnant sows at the age of 25-114 days after mating are divided into four groups of 80 sows, a total of 320 positioning fences are fed by adopting the positioning fences, 2 percent of feed additives of the embodiment 1 and the comparative examples 1-3 are respectively added into the feed, sufficient drinking water is supplied, limited feeding is adopted to feed two meals at regular time and fixed quantity every day according to the body condition of the sows, the total experiment lasts for 90 days, the constipation condition of the sows, the tear spot condition of the sows, the birth weight of piglets and the litter size of the piglets are counted and shown in the table 4.
TABLE 4
As can be seen from Table 4, the group fed with the feed additive provided in example 1 is obviously higher than the comparative examples 1 and 3 in improving the constipation rate and the tear spot rate of sows, and the number of healthy piglets and the average litter size of healthy piglets are also higher than the comparative examples 1 and 3, which shows that the growth condition of sows can be obviously improved, the litter size can be increased, and the economic benefit of farmers can be increased by adding the feed additive provided by the application into the feed of sows and compounding the riboflavin fermentation waste liquid, the folium artemisiae argyi and the marigold residues.
The test results show that the immunity of the pigs can be obviously improved by using the matched riboflavin fermentation waste liquid, folium artemisiae argyi and marigold residues, the disease resistance is increased, the growth condition of the sows can be obviously improved, the oestrus rate and the farrowing rate of the sows are improved, and the comparison with the comparative example 1, the comparative example 2 and the comparative example 3 shows that the effect of the compound preparation is inferior to that of the example 1 after the traditional Chinese medicine components are omitted and the riboflavin fermentation waste liquid is independently added or only the traditional Chinese medicine components are omitted and the riboflavin fermentation waste liquid is replaced by vitamin B2, so that the compound preparation has the synergistic effect of the riboflavin fermentation waste liquid, the folium artemisiae argyi and the marigold residues in the formula, and the compound preparation has the advantages of mutual gain, promotes the oestrus growth of the sows, reduces the morbidity, improves the farrowing rate, further improves the economic benefit of farmers and reduces the breeding cost.
The above embodiments are only preferred embodiments of the present invention, and the protection scope of the present invention is not limited thereby, and any insubstantial changes and substitutions made by those skilled in the art based on the present invention are within the protection scope of the present invention.
Claims (5)
1. The pig feed additive containing the riboflavin fermentation waste liquid is characterized by being prepared from the following raw materials: 30-60 parts of riboflavin fermentation waste liquid, 30-50 parts of folium artemisiae argyi, 5-60 parts of marigold slag, 0.1-0.3 part of preservative, 0.5-1 part of sweetening agent and 0.5-1 part of mildew removing agent;
the riboflavin fermentation waste liquid is waste liquid remained in the process of preparing riboflavin by a fermentation method;
the preparation method of the pig feed additive comprises the following steps:
(1) Pulverizing folium Artemisiae Argyi and flos Tagetis Erectae residue, adding into riboflavin fermentation waste liquid, stirring, mixing, and fermenting at 40-50 deg.C for 40-48 hr;
(2) Drying the mixture fermented in the step (1) until the water content is 35-65%;
(3) And (3) adding the preservative, the mildew removing agent and the sweetening agent into the mixture dried in the step (2) and uniformly stirring to obtain the pig feed additive.
2. The swine feed additive containing riboflavin fermentation waste liquid according to claim 1, wherein the swine feed additive comprises the following raw materials: 50 parts of riboflavin fermentation waste liquid, 45 parts of folium artemisiae argyi, 5 parts of marigold slag, 0.2 part of preservative, 0.5 part of sweetening agent and 0.5 part of mildew removing agent.
3. The swine feed additive containing riboflavin fermentation waste according to claim 1, wherein the preservative is sodium benzoate.
4. The swine feed additive containing riboflavin fermentation waste liquid according to claim 1, wherein the sweetener is saccharin sodium.
5. The swine feed additive containing riboflavin fermentation waste liquor according to claim 1, wherein said mold removing agent is aflatoxin decomposition enzyme.
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| CN112385745A (en) * | 2020-11-12 | 2021-02-23 | 孟祥宾 | Pig feed additive containing antibacterial peptide riboflavin fermentation broth traditional Chinese medicine |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4165250A (en) * | 1975-08-29 | 1979-08-21 | Merck & Co., Inc. | Riboflavin purification |
| CN1305731A (en) * | 2001-02-05 | 2001-08-01 | 广西大学 | Feed for fowls and livestock and its preparing process |
| CN103815144A (en) * | 2014-03-17 | 2014-05-28 | 双胞胎（集团）股份有限公司 | Mycotoxin removal feed additive for pigs |
| CN104431560A (en) * | 2014-12-15 | 2015-03-25 | 福建傲农生物科技集团有限公司 | Pig feed additive with efficacies of protecting intestinal tracts and improving food calling function |
| CN104489392A (en) * | 2014-12-19 | 2015-04-08 | 天津万达嘉禾饲料有限公司 | Selenium-rich feed for pigs and preparation method thereof |
| CN105230982A (en) * | 2015-09-30 | 2016-01-13 | 河南科技大学 | Fermented feed for animals, and preparation method thereof |
| CN106036018A (en) * | 2016-06-24 | 2016-10-26 | 界首市起点家庭农场 | Pig feed with disease prevention function |
| CN107616352A (en) * | 2017-11-06 | 2018-01-23 | 安庆市立华牧业有限公司 | Improve mixed feed of chicken immunity and preparation method thereof |
-
2018
- 2018-05-08 CN CN201810430284.6A patent/CN108308431B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4165250A (en) * | 1975-08-29 | 1979-08-21 | Merck & Co., Inc. | Riboflavin purification |
| CN1305731A (en) * | 2001-02-05 | 2001-08-01 | 广西大学 | Feed for fowls and livestock and its preparing process |
| CN103815144A (en) * | 2014-03-17 | 2014-05-28 | 双胞胎（集团）股份有限公司 | Mycotoxin removal feed additive for pigs |
| CN104431560A (en) * | 2014-12-15 | 2015-03-25 | 福建傲农生物科技集团有限公司 | Pig feed additive with efficacies of protecting intestinal tracts and improving food calling function |
| CN104489392A (en) * | 2014-12-19 | 2015-04-08 | 天津万达嘉禾饲料有限公司 | Selenium-rich feed for pigs and preparation method thereof |
| CN105230982A (en) * | 2015-09-30 | 2016-01-13 | 河南科技大学 | Fermented feed for animals, and preparation method thereof |
| CN106036018A (en) * | 2016-06-24 | 2016-10-26 | 界首市起点家庭农场 | Pig feed with disease prevention function |
| CN107616352A (en) * | 2017-11-06 | 2018-01-23 | 安庆市立华牧业有限公司 | Improve mixed feed of chicken immunity and preparation method thereof |
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