CN108300640B - 一种用于核酸自动化提取和检测的微流控芯片 - Google Patents
一种用于核酸自动化提取和检测的微流控芯片 Download PDFInfo
- Publication number
- CN108300640B CN108300640B CN201810045288.2A CN201810045288A CN108300640B CN 108300640 B CN108300640 B CN 108300640B CN 201810045288 A CN201810045288 A CN 201810045288A CN 108300640 B CN108300640 B CN 108300640B
- Authority
- CN
- China
- Prior art keywords
- micro
- cavity
- fluidic chip
- main body
- interface
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 39
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 39
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 39
- 238000001514 detection method Methods 0.000 title claims abstract description 18
- 238000000605 extraction Methods 0.000 title claims abstract description 18
- 239000000758 substrate Substances 0.000 claims abstract description 11
- 238000004140 cleaning Methods 0.000 claims description 84
- 239000007788 liquid Substances 0.000 claims description 75
- 238000003860 storage Methods 0.000 claims description 52
- 239000000243 solution Substances 0.000 claims description 35
- 238000002347 injection Methods 0.000 claims description 34
- 239000007924 injection Substances 0.000 claims description 34
- 238000002156 mixing Methods 0.000 claims description 26
- 239000000203 mixture Substances 0.000 claims description 26
- 239000003480 eluent Substances 0.000 claims description 25
- 238000006243 chemical reaction Methods 0.000 claims description 22
- 239000003960 organic solvent Substances 0.000 claims description 22
- 239000012295 chemical reaction liquid Substances 0.000 claims description 21
- 239000002699 waste material Substances 0.000 claims description 20
- 239000011324 bead Substances 0.000 claims description 13
- 239000000463 material Substances 0.000 claims description 9
- 230000003321 amplification Effects 0.000 claims description 8
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 8
- 238000001821 nucleic acid purification Methods 0.000 claims description 8
- 239000003153 chemical reaction reagent Substances 0.000 claims description 6
- 238000010438 heat treatment Methods 0.000 claims description 4
- -1 polydimethylsiloxane Polymers 0.000 claims description 4
- 239000002861 polymer material Substances 0.000 claims description 4
- 239000011259 mixed solution Substances 0.000 claims description 3
- 239000000126 substance Substances 0.000 claims description 3
- 229920000089 Cyclic olefin copolymer Polymers 0.000 claims description 2
- 239000004713 Cyclic olefin copolymer Substances 0.000 claims description 2
- 239000004743 Polypropylene Substances 0.000 claims description 2
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims description 2
- 239000011203 carbon fibre reinforced carbon Substances 0.000 claims description 2
- 239000004205 dimethyl polysiloxane Substances 0.000 claims description 2
- 239000012530 fluid Substances 0.000 claims description 2
- 239000011521 glass Substances 0.000 claims description 2
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 claims description 2
- 229920003229 poly(methyl methacrylate) Polymers 0.000 claims description 2
- 229920000515 polycarbonate Polymers 0.000 claims description 2
- 239000004417 polycarbonate Substances 0.000 claims description 2
- 239000004926 polymethyl methacrylate Substances 0.000 claims description 2
- 229920001155 polypropylene Polymers 0.000 claims description 2
- 239000010453 quartz Substances 0.000 claims description 2
- 229910052710 silicon Inorganic materials 0.000 claims description 2
- 239000010703 silicon Substances 0.000 claims description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 2
- 229920001169 thermoplastic Polymers 0.000 claims description 2
- 238000012864 cross contamination Methods 0.000 abstract description 2
- 238000003752 polymerase chain reaction Methods 0.000 description 12
- 238000010828 elution Methods 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- 230000009089 cytolysis Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000011541 reaction mixture Substances 0.000 description 3
- 238000012408 PCR amplification Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000001917 fluorescence detection Methods 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000006037 cell lysis Effects 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000002558 medical inspection Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/12—Specific details about manufacturing devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Dispersion Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
Claims (5)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810045288.2A CN108300640B (zh) | 2018-01-17 | 2018-01-17 | 一种用于核酸自动化提取和检测的微流控芯片 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810045288.2A CN108300640B (zh) | 2018-01-17 | 2018-01-17 | 一种用于核酸自动化提取和检测的微流控芯片 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN108300640A CN108300640A (zh) | 2018-07-20 |
CN108300640B true CN108300640B (zh) | 2021-11-26 |
Family
ID=62865750
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201810045288.2A Active CN108300640B (zh) | 2018-01-17 | 2018-01-17 | 一种用于核酸自动化提取和检测的微流控芯片 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN108300640B (zh) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109985680A (zh) * | 2019-04-24 | 2019-07-09 | 浙江警察学院 | 适用于手持式spr检测仪的模块化多通道样品检测芯片组件 |
CN111073811A (zh) * | 2019-12-24 | 2020-04-28 | 深圳市华迈生物医疗科技有限公司 | 一种用于实时荧光核酸扩增检测的微流控芯片及检测方法 |
CN111804280A (zh) * | 2020-06-11 | 2020-10-23 | 中国科学院合肥物质科学研究院 | 一种制备磁性纳米氨基复合物的方法、以及核酸提取方法 |
CN113025476A (zh) * | 2020-06-17 | 2021-06-25 | 山东大学 | 一种双层微流控芯片、检测新型冠状病毒的试剂盒及方法 |
CN112175820B (zh) * | 2020-10-10 | 2022-09-02 | 上海世艾生物科技有限公司 | 一种核酸提取和pcr一体化装置及其工作方法 |
CN113832027A (zh) * | 2021-11-01 | 2021-12-24 | 上海邦先医疗科技有限公司 | 一种用于pcr检测的立式微流控芯片及方法 |
CN114062632B (zh) * | 2021-11-17 | 2023-12-29 | 扬州大学 | 一种基于纳米颗粒酶联增敏的镉离子微流控检测方法 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104232469A (zh) * | 2014-09-11 | 2014-12-24 | 北京化工大学 | 基于磁珠的样品处理及核酸自动提取*** |
CN105296349A (zh) * | 2015-11-20 | 2016-02-03 | 青岛意诚融智生物仪器有限公司 | 一种用于dna快速检测的微流控芯片、检测***和装置 |
CN105349401A (zh) * | 2015-10-14 | 2016-02-24 | 安徽易康达光电科技有限公司 | 一种多功能的集成化微流控核酸分析芯片及制备和分析方法 |
CN107541452A (zh) * | 2017-09-20 | 2018-01-05 | 清华大学 | 一种微流控芯片及芯片组件 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20050118647A1 (en) * | 2001-12-31 | 2005-06-02 | Yung-Chiang Chung | Microfluidic mixer apparatus and microfluidic reactor apparatus for microfluidic processing |
US20150361419A1 (en) * | 2013-01-21 | 2015-12-17 | Nanobiosys Inc. | Microfluidic chip for extracting nucleic acids, device for extracting nucleic acids comprising same, and method for extracting nucleic acids using same |
-
2018
- 2018-01-17 CN CN201810045288.2A patent/CN108300640B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104232469A (zh) * | 2014-09-11 | 2014-12-24 | 北京化工大学 | 基于磁珠的样品处理及核酸自动提取*** |
CN105349401A (zh) * | 2015-10-14 | 2016-02-24 | 安徽易康达光电科技有限公司 | 一种多功能的集成化微流控核酸分析芯片及制备和分析方法 |
CN105296349A (zh) * | 2015-11-20 | 2016-02-03 | 青岛意诚融智生物仪器有限公司 | 一种用于dna快速检测的微流控芯片、检测***和装置 |
CN107541452A (zh) * | 2017-09-20 | 2018-01-05 | 清华大学 | 一种微流控芯片及芯片组件 |
Non-Patent Citations (4)
Title |
---|
A microfluidic chip integrating DNA extraction and real-time PCR for the detection of bacteria in saliva;Emily A. Oblath et al.;《Lab Chip》;20130111;第13卷;第1325-1332页 * |
Microfluidic chip for stacking, separation and extraction of multiple DNA fragments;Ruige Wu et al.;《Journal of Chromatography A》;20160203;第1437卷;第219-225页 * |
全国普通高等院校生物医学工程规划教材 生物医学工程技术;顾月清 等;《全国普通高等院校生物医学工程规划教材 生物医学工程技术》;中国医药科技出版社;20170630;第217-219页 * |
新编法医物证检验技术;童大跃 等;《新编法医物证检验技术》;中国医药科技出版社;20131130;第196-197页 * |
Also Published As
Publication number | Publication date |
---|---|
CN108300640A (zh) | 2018-07-20 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN108300640B (zh) | 一种用于核酸自动化提取和检测的微流控芯片 | |
CN105170206B (zh) | 一种多指标检测的微流控芯片 | |
CN101990516B (zh) | 多用试样准备***及其在集成分析***中的使用 | |
US8343443B2 (en) | Fluid processing and transfer using inter-connected multi-chamber device | |
CN111760601B (zh) | 一种集成液路切换阀的微流控芯片及核酸检测方法 | |
CN112195099B (zh) | 一种用于核酸检测的微流控芯片 | |
CN104762193A (zh) | 核酸自动提取微流控装置 | |
US10246675B2 (en) | Biochemical cartridge, and biochemical cartridge and cartridge holder set | |
KR20120051709A (ko) | 미세유체 장치 및 이의 용도 | |
WO2022174471A1 (zh) | 一种全集成病原体核酸检测微流控芯片 | |
US11478795B2 (en) | Microfluidic device and method for analyzing nucleic acids | |
CN219792985U (zh) | 检测核酸的芯片装置和仪器 | |
CN115678771B (zh) | 一种多通道分子诊断的微流控芯片 | |
CN114182000B (zh) | 一种基于crispr技术的一体化核酸检测芯片及方法 | |
CN103484353A (zh) | 基于滤纸的生物大分子提取装置 | |
CN111389474B (zh) | 一种用于样本分散的微流控芯片及其制备方法与应用 | |
CN110616142B (zh) | 一种用于便携设备的核酸检测芯片及其使用方法 | |
CN113717827B (zh) | 一种全集成核酸检测微流控芯片及使用方法 | |
CN115400806A (zh) | 一体化核酸提取微流控芯片盒和核酸提取及检测方法 | |
CN117384750B (zh) | 一种全集成数字化核酸分析卡盒 | |
CN218890576U (zh) | 一体化核酸提取微流控芯片盒 | |
CN216550429U (zh) | 一种核酸检测芯片 | |
CN114768894B (zh) | 一种检测芯片及检测方法 | |
CN113773951A (zh) | 一种核酸检测芯片 | |
Han et al. | Integrated interface technology for microfluidic systems |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20231225 Address after: 230031 Shushan Lake Road, Shushan District, Hefei, Anhui 350 Patentee after: HEFEI INSTITUTES OF PHYSICAL SCIENCE, CHINESE ACADEMY OF SCIENCES Patentee after: Anhui Zhongke Yikangda Biotechnology Co.,Ltd. Address before: 230031 No. 350 Lake Road, Hefei, Anhui, Shushan Patentee before: HEFEI INSTITUTES OF PHYSICAL SCIENCE, CHINESE ACADEMY OF SCIENCES Patentee before: HEFEI ZHONGKE YIKANGDA BIOMEDICAL Co.,Ltd. |
|
TR01 | Transfer of patent right |