CN108264399A - A kind of preparation method of agricultural amino acid fertilizer - Google Patents
A kind of preparation method of agricultural amino acid fertilizer Download PDFInfo
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- CN108264399A CN108264399A CN201810112582.0A CN201810112582A CN108264399A CN 108264399 A CN108264399 A CN 108264399A CN 201810112582 A CN201810112582 A CN 201810112582A CN 108264399 A CN108264399 A CN 108264399A
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- amino acid
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- agricultural amino
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/13—Brevibacterium
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- General Health & Medical Sciences (AREA)
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- Fertilizers (AREA)
Abstract
The invention discloses a kind of preparation method of agricultural amino acid fertilizer, raw material is made of soy meal and cornstarch；The raw material prepares agricultural amino acid fertilizer after being saccharified pretreated saccharified liquid and strain liquid mixed fermentation.Raw material of the present invention is formed less, simple production process, and the yield of total amino acid is up to more than 37% in prepared zymotic fluid, is efficient agricultural ammino acid liquid fertilizer, and has high-efficiency broad spectrum disease resistance, and solids prepares high-quality organic fertilizer, and raw material is all of without byproduct.
Description
Technical field
Preparation method more particularly to a kind of preparation method of agricultural amino acid fertilizer the present invention relates to a kind of fertilizer.
Background technology
Agricultural amino acid fertilizer is gradually approved due to many advantages, such as fertilizer efficiency is high, utilization rate of fertilizer is high by user.Such as
ZL2004100047377, a kind of agricultural amino acid, disclosed in technical solution, raw material is using plant cake, glutamate production tail
Filtered fluid, animal protein and the commodity of liquid expand bacterium, and plant cake and animal protein are mixed, and add in commodity and expand bacterium 45-65
DEG C fermentation 120-180h, low temperature drying after fermentation is dry that object is uniformly mixed with the filtered fluid of glutamate production tail washings, dry, powder
Broken, screening obtains.
There are many existing production technology, do not enumerate one by one, and the scheme disclosed in these technologies is more there are raw material, and technique is multiple
Miscellaneous, low yield leads to the problem of of high cost, limits the popularization of agricultural amino acid Fertilizer application.
Invention content
In order to solve the problems, such as current techniques, the object of the present invention is to provide a kind of systems of agricultural amino acid fertilizer
Preparation Method.
In order to achieve the above objectives, the technology used in the present invention means are：A kind of preparation method of agricultural amino acid fertilizer,
Raw material is made of soy meal and cornstarch.
Further, the raw material prepares agricultural amino after being saccharified pretreated saccharified liquid and strain liquid mixed fermentation
Sour fertilizer.
Further, the saccharification pretreatment refers to：After soy meal and cornstarch are mixed, carbohydrase, sugar are added in
Change 30~70h of time, 30~35 DEG C of temperature, wherein shared parts by weight are：Soy meal 30~70, corn flour dosage 30~70, sugar
Change enzyme dosage 1~7, deionized water 40~80.
Further, the soy meal and cornstarch cross 80 mesh sieve.
Further, the strain is brevibacterium flavum.
Further, the preparation method of the strain liquid is：Brevibacterium flavum is through test tube, triangular flask, one cubic metre of hair
Fermentation tank three-level expands culture, using the tryptone containing 4%NaCl+soya bean peptone agar+soya bean juice culture medium, fermentation temperature 20~
It 25 DEG C, is all controlled in 60~80h per level-one fermentation time.
Further, the saccharified liquid refers to strain liquid mixed fermentation：Strain liquid, setting temperature are added in into saccharified liquid
20~25 DEG C of degree, ferment 60~80h, wherein volume ratio, saccharified liquid 90~95%, strain liquid 5~10%.
Further, bacterial strain content is 500,000,000/ml in the strain liquid.
Further, the agricultural amino acid fertilizer for preparing refers to：Gained zymotic fluid plate and frame filter press press filtration solid-liquid
Separation, filtrate negative pressure low temperature are concentrated to give liquid portion, and total amino acid is more than 37% in liquid portion, applies fertilizer for rushing, drip irrigation fertilizer,
Top dressing, foliar fertilizer or addition N P and K inorganic fertilizer prepare compound organic and inorganic fertilizer；It is directly obtained after solid portion drying organic
Fertilizer is applied for base fertilizer.
The beneficial effects of the present invention are：Raw material is formed less, simple production process, total amino acid in prepared zymotic fluid
Yield be up to more than 37%, be efficient agricultural ammino acid liquid fertilizer, and with high-efficiency broad spectrum disease resistance, prepared by solids high-quality have
Machine fertilizer, raw material is all of without byproduct.
Specific embodiment
Embodiment 1
A kind of preparation method of agricultural amino acid fertilizer, raw material are made of soy meal and cornstarch, and the raw material is pre- through being saccharified
Treated saccharified liquid and prepare agricultural amino acid fertilizer after strain liquid mixed fermentation.
The present embodiment raw material composition only needs soy meal and cornstarch, and processing step is simple.
Embodiment 2
A kind of specific presentation of step as embodiment 1, the saccharification pretreatment refer to：Soy meal and cornstarch are mixed
Afterwards, carbohydrase, 30~70h of saccharificatinn period, 30~35 DEG C of temperature, wherein shared parts by weight are are added in：Soy meal 30~70, corn
Powder dosage 30~70, be saccharified enzyme dosage 1~7, deionized water 40~80.
Embodiment 3
Preferably, the soy meal and cornstarch cross 80 mesh sieve to one kind as embodiment 2.
This preferred embodiment can promote the effect and product of saccharification processing.
Embodiment 4
A kind of specific presentation of step as embodiment 1, the strain are brevibacterium flavum.
The preparation method of the strain liquid is：Brevibacterium flavum expands through test tube, triangular flask, one cubic metre of fermentation tank three-level
Culture, using the tryptone containing 4%NaCl+soya bean peptone agar+soya bean juice culture medium, 20~25 DEG C of fermentation temperature is sent out per level-one
The ferment time is all controlled in 60~80h.
The present embodiment discloses the method that strain liquid spreads cultivation in industrialized production, the strain mother liquor prepared after three grade fermemtation
It can meet the needs of production.
Embodiment 5
A kind of specific presentation of step as embodiment 1, the saccharified liquid refer to strain liquid mixed fermentation：Into saccharified liquid
Strain liquid is added in, 20~25 DEG C of set temperature, ferment 60~80h, wherein volume ratio saccharified liquid 90~95%, strain liquid 5~10%.
Bacterial strain content is 500,000,000/ml in the strain liquid.
Bacterial strain content in control saccharified liquid, the volume ratio of strain liquid and strain liquid, product meets technology after ensureing fermentation
Demand.
Embodiment 6
A kind of specific presentation of step as embodiment 1, the agricultural amino acid fertilizer for preparing refer to：Gained zymotic fluid plate
Frame filter press press filtration separation of solid and liquid, filtrate negative pressure low temperature are concentrated to give liquid portion, and total amino acid is more than 37% in liquid portion, uses
It applies fertilizer in punching, drip irrigation fertilizer, top dressing, foliar fertilizer or addition N P and K inorganic fertilizer prepare compound organic and inorganic fertilizer；Solid portion is dried
After directly obtain organic fertilizer, for base fertilizer apply.
It is presented by above-described embodiment, it can be seen that raw material of the present invention forms few, simple production process, prepared hair
The yield of total amino acid is up to more than 37% in zymotic fluid, is efficient agricultural ammino acid liquid fertilizer so that plant is disease-resistant with high-efficiency broad spectrum
Property, solids prepare high-quality organic fertilizer, and raw material is all of without byproduct.
Application embodiment is only intended to illustrate technical characteristic disclosed in the present application, and those skilled in the art pass through simple
Carried out change is replaced, still falls within the range that the application is protected.
Claims (9)
1. a kind of preparation method of agricultural amino acid fertilizer, it is characterised in that：Raw material is made of soy meal and cornstarch.
2. the preparation method of agricultural amino acid fertilizer according to claim 1, it is characterised in that：The raw material is pre- through being saccharified
Treated saccharified liquid and prepare agricultural amino acid fertilizer after strain liquid mixed fermentation.
3. the preparation method of agricultural amino acid fertilizer according to claim 2, it is characterised in that：The saccharification pre-processes
Refer to：After soy meal and cornstarch are mixed, carbohydrase, 30~70h of saccharificatinn period, 30~35 DEG C of temperature, wherein shared are added in
Parts by weight are：Soy meal 30~70, corn flour dosage 30~70, be saccharified enzyme dosage 1~7, deionized water 40~80.
4. the preparation method of agricultural amino acid fertilizer according to claim 3, it is characterised in that：The soy meal and corn
Starch crosses 80 mesh sieve.
5. the preparation method of agricultural amino acid fertilizer according to claim 2, it is characterised in that：The strain is short for yellow
Bacillus.
6. the preparation method of agricultural amino acid fertilizer according to claim 5, it is characterised in that：The preparation of the strain liquid
Method is：Brevibacterium flavum is through test tube, and triangular flask, one cubic metre of fermentation tank three-level expands culture, using the pancreas egg containing 4%NaCl
White peptone+soya bean peptone agar+soya bean juice culture medium, 20~25 DEG C of fermentation temperature are all controlled per level-one fermentation time in 60~80h.
7. the preparation method of agricultural amino acid fertilizer according to claim 2, it is characterised in that：The saccharified liquid and strain
Liquid mixed fermentation refers to：Strain liquid is added in into saccharified liquid, 20~25 DEG C of set temperature, ferment 60~80h, wherein volume ratio,
Saccharified liquid 90~95%, strain liquid 5~10%.
8. the preparation method of agricultural amino acid fertilizer according to claim 7, it is characterised in that：Bacterial strain in the strain liquid
Content is 500,000,000/ml.
9. the preparation method of agricultural amino acid fertilizer according to claim 2, it is characterised in that：It is described to prepare agricultural amino
Sour fertilizer refers to：Gained zymotic fluid plate and frame filter press press filtration separation of solid and liquid, filtrate negative pressure low temperature are concentrated to give liquid portion, liquid
Total amino acid is more than 37% in part, applies fertilizer for rushing, drip irrigation fertilizer, top dressing, and foliar fertilizer or the inorganic fertilizer preparation of addition N P and K have
Machine inorganic composite mixed fertilizer；Organic fertilizer is directly obtained after solid portion drying, is applied for base fertilizer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810112582.0A CN108264399A (en) | 2018-02-05 | 2018-02-05 | A kind of preparation method of agricultural amino acid fertilizer |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810112582.0A CN108264399A (en) | 2018-02-05 | 2018-02-05 | A kind of preparation method of agricultural amino acid fertilizer |
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| Publication Number | Publication Date |
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| CN108264399A true CN108264399A (en) | 2018-07-10 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201810112582.0A Pending CN108264399A (en) | 2018-02-05 | 2018-02-05 | A kind of preparation method of agricultural amino acid fertilizer |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108840779A (en) * | 2018-08-10 | 2018-11-20 | 芜湖响民生态农业有限公司 | A kind of vegetable cultivation organic and inorganic base fertilizer and preparation method thereof |
| CN108863583A (en) * | 2018-08-10 | 2018-11-23 | 芜湖响民生态农业有限公司 | A kind of Rice Cropping organic and inorganic base fertilizer and preparation method thereof |
| CN108863582A (en) * | 2018-08-10 | 2018-11-23 | 芜湖响民生态农业有限公司 | A kind of winter vegetable plantation organic and inorganic base fertilizer and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104561162A (en) * | 2013-10-23 | 2015-04-29 | 中粮营养健康研究院有限公司 | Method for preparing lysine by fermentation |
| CN104726510A (en) * | 2013-12-24 | 2015-06-24 | 中粮生物化学（安徽）股份有限公司 | Method for preparing lysine through fermenting |
| CN105154358A (en) * | 2015-08-19 | 2015-12-16 | 华南理工大学 | Bacillus and method for simultaneous saccharification and fermentation production of L-lactic acid with same |
| CN106148443A (en) * | 2015-04-02 | 2016-11-23 | 中粮生物化学（安徽）股份有限公司 | A kind of method producing lysine |
| CN106396879A (en) * | 2016-08-27 | 2017-02-15 | 安徽省司尔特肥业股份有限公司 | Soil improvement type selenium-rich special slow release fertilizer for soybeans, and preparation method thereof |
-
2018
- 2018-02-05 CN CN201810112582.0A patent/CN108264399A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104561162A (en) * | 2013-10-23 | 2015-04-29 | 中粮营养健康研究院有限公司 | Method for preparing lysine by fermentation |
| CN104726510A (en) * | 2013-12-24 | 2015-06-24 | 中粮生物化学（安徽）股份有限公司 | Method for preparing lysine through fermenting |
| CN106148443A (en) * | 2015-04-02 | 2016-11-23 | 中粮生物化学（安徽）股份有限公司 | A kind of method producing lysine |
| CN105154358A (en) * | 2015-08-19 | 2015-12-16 | 华南理工大学 | Bacillus and method for simultaneous saccharification and fermentation production of L-lactic acid with same |
| CN106396879A (en) * | 2016-08-27 | 2017-02-15 | 安徽省司尔特肥业股份有限公司 | Soil improvement type selenium-rich special slow release fertilizer for soybeans, and preparation method thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108840779A (en) * | 2018-08-10 | 2018-11-20 | 芜湖响民生态农业有限公司 | A kind of vegetable cultivation organic and inorganic base fertilizer and preparation method thereof |
| CN108863583A (en) * | 2018-08-10 | 2018-11-23 | 芜湖响民生态农业有限公司 | A kind of Rice Cropping organic and inorganic base fertilizer and preparation method thereof |
| CN108863582A (en) * | 2018-08-10 | 2018-11-23 | 芜湖响民生态农业有限公司 | A kind of winter vegetable plantation organic and inorganic base fertilizer and preparation method thereof |
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Application publication date: 20180710 |
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| RJ01 | Rejection of invention patent application after publication |