CN108244632A - A kind of multifunctional solid health food and preparation method thereof - Google Patents
A kind of multifunctional solid health food and preparation method thereof Download PDFInfo
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- CN108244632A CN108244632A CN201810126474.9A CN201810126474A CN108244632A CN 108244632 A CN108244632 A CN 108244632A CN 201810126474 A CN201810126474 A CN 201810126474A CN 108244632 A CN108244632 A CN 108244632A
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- health food
- probiotics
- multifunctional solid
- powder
- solid health
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Classifications
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- A—HUMAN NECESSITIES
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- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/20—Agglomerating; Granulating; Tabletting
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/30—Encapsulation of particles, e.g. foodstuff additives
-
- A—HUMAN NECESSITIES
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Abstract
The present invention relates to a kind of multifunctional solid health food, according to mass percent meter by following material composition：Cordyceps militaris powder 30~35%, probiotics spray dried powder 65~70%；The probiotics spray dried powder is that gained zymotic fluid is spray-dried to be made by lactobacillus plantarum seed liquor, Lactobacillus casei seed liquor and lactobacillus acidophilus seed liquor after mixed fermentation.The invention further relates to a kind of preparation methods of multifunctional solid health food.Multifunctional solid health food provided by the invention, can not only strengthen immunity function, moreover it is possible to prevent cardiovascular and cerebrovascular disease and protection liver function, be a kind of multi-functional health food；Simultaneously using solid dosage (powder or capsule), carry consumer and take all easily.
Description
Technical field
The invention belongs to functional health product fields, and in particular to a kind of multifunctional solid health food and its preparation side
Method.
Background technology
In the modern life, with diet, living habit, the variation of contact environment, cardiovascular and cerebrovascular disease crowd quantity is not
Disconnected to increase, only in China, at present, Patients with Cardiovascular/Cerebrovascular Diseases alreadys exceed 2.7 hundred million people.China dies of cardiovascular and cerebrovascular disease every year
Nearly 3,000,000 people, account for China every year total Death causes 51%, and there is gradual rejuvenation.Simultaneously, due to drug resistance
The continuous generation of property microorganism, the health threat caused by pathogenic microorganism infection increasingly increases, because immunity degradation causes to feel
Crowd's quantity of dye or even initiation malignant tumour also increases rapidly, and treats drug used in above-mentioned health problem, and can be right
Liver causes toxic side effect, and correlated crowd is made further to face the threat of hepatic injury.
It may be said that above-mentioned two classes disease has become the great threat for human health, people can be adjusted simultaneously there is an urgent need to a kind of
Immune system, prevention of cardiovascular disease and liver-protective nutraceutical.And health food is although various in style at present, but mostly
Number can only play the role of in a certain respect.
Invention content
The present invention overcomes deficiencies of the prior art, are that providing one kind is provided simultaneously with strengthen immunity function, prevention
Multifunctional solid health food of cardiovascular and cerebrovascular disease and protection liver function and preparation method thereof.
The specific technical solution of the present invention is as follows：
A kind of multifunctional solid health food, according to mass percent meter by following material composition：Cordyceps militaris powder 30~
35%, probiotics spray dried powder 65~70%；The probiotics spray dried powder is by lactobacillus plantarum seed liquor, cheese breast
After mixed fermentation, gained zymotic fluid is spray-dried to be made for bacillus seed liquor and lactobacillus acidophilus seed liquor.
The present invention prepares multifunctional solid health food, the freeze-drying of relatively traditional probiotics using probiotics spray dried powder
Technology due to not taking centrifugal method, retains all supernatants, probiotics spray drying process can more retain probiotics fermention
The active ingredients such as short chain fatty acids, peptide glycan, nucleic acid fragment in liquid.
Connecing when the lactobacillus plantarum seed liquor, Lactobacillus casei seed liquor and lactobacillus acidophilus seed liquor mixed fermentation
Kind amount is by volume 1：1~2：1, the preferred lactobacillus plantarum seed liquor, Lactobacillus casei seed liquor and acidophilus breast bar
Inoculum concentration during bacterium seed liquor three's mixed fermentation is by volume 1：2：1；During three kinds of strain seed liquor mixed fermentation, using institute
The ratio of stating can ensure that three strains all quickly enter exponential phase (i.e. fast breeder phase), reach the bacterium number of final zymotic fluid
To highest.
Preferably, the probiotics spray dried powder is prepared with the following method：
Lactobacillus plantarum, Lactobacillus casei and lactobacillus acidophilus are inoculated in respectively through autoclaved seed culture medium
In, 37 DEG C are cultivated 12~16 hours, and primary seed solution is made；The primary seed solution of three strains is by volume respectively 1: 10 inoculation
Enter through in autoclaved seed culture medium, 37 DEG C are cultivated 12~16 hours, and secondary seed solution is made；Lactobacillus plantarum, acidophilus
The secondary seed solution of lactobacillus is using volume ratio as 1：2000 ratio, the secondary seed solution of Lactobacillus casei are using volume ratio as 1:
Access is obtained prebiotic through in autoclaved fermentation medium, 37 DEG C are cultivated 12~16 hours jointly for 1000~2000 ratio
Fermented liquid；After probiotics fermention liquid obtained is concentrated in vacuo to the 20~25% of original volume, concentrate, concentrate warp are obtained
Spray drying obtains probiotics spray dried powder.
The present invention is conventional by lactobacillus plantarum, Lactobacillus casei and lactobacillus acidophilus slant strains by microbiological manipulations first
It is inoculated in respectively through in autoclaved seed culture medium, being cultivated using amplification and seed liquor, each seed liquor mixed fermentation being made
It is made probiotics fermention liquid afterwards, exactly in order to allow the ingredient that strain can in the process in continuous acclimatizing culture medium, makes finally to send out
Ferment is rapider, thalline and beneficiating ingredient concentration higher in probiotics fermention liquid.
Preferably, microcrystalline cellulose is added in into concentrate, 200~250g microcrystalline celluloses are added in every liter of concentrate,
It is spray-dried to obtain probiotics spray dried powder；The microcrystalline cellulose is added in concentrate, to improve concentrate physics
Property reduces glutinous wall phenomenon of the concentrate in spray-drying process, so as to improve spray-drying efficiency；Adsorb simultaneously protected fermentation
Liquid includes the functional components such as thalline, organic acid, cell wall, reduces spray drying temperature variation damage caused by these ingredients
It loses.
In order to ensure to reach higher thickening efficiency on the basis of functional component, activity, it is preferred that pressure during vacuum concentration
Power is -0.1~-0.08Mpa, and feed temperature is 73~83 DEG C.
Preferably, inlet air temperature is 150~170 DEG C during spray drying, and leaving air temp is 70~90 DEG C；Select the air inlet
Temperature is that concentrate sticks wall in order to prevent, while takes into account spray-drying efficiency and preserve active constituent；Selection it is described it is relatively low go out
Air temperature is the loss in order to reduce active constituent in spray dried powder.
Preferably, the seed culture medium, fermentation medium according to mass percent meter by following material composition：Soybean
Plumule 2.5-3.0%, peanut germ 1.0-1.5%, lotus seeds 3.5-4.0%, jujube 1.0-1.5%, matrimony vine 0.4-0.6%, Fu
Siberian cocklebur 0.4-0.6%, dusty yeast 0.1-0.3%, glucose 0.8-1.2%, soda ash 0.1-0.15%, surplus are water.The culture medium
Based on natural plant composition, wherein soybean germ and peanut germ contain abundant nutriment, and the growth for probiotics carries
While for abundant nutriment, and make to be rich in amino acid and vitamin in final zymotic fluid；Jujube, matrimony vine, Poria cocos are medicine food
Homologous healthy food, has the function of tonifying liver invigorating the spleen, the healthcare function of cooperation probiotics in itself, increases zymotic fluid or even final
The health-care efficacy of finished product.
Cordyceps militaris powder of the present invention may be used existing method and be prepared.
The invention further relates to the preparation methods of the multifunctional solid health food, include the following steps：Cordyceps militaris powder
It is sieved with probiotics spray dried powder mixed material through 80 mesh, capsule is prepared or granule filling prepares powder using capsule is filling.
It is edible for convenience, it is preferred that the capsule filling amount is 0.3 gram, 0.5 gram, 0.8 gram, 1.0 grams.
It is edible for convenience, it is preferred that the powder filling amount is 1 gram, 2 grams, 50 grams.
Lactobacillus casei, lactobacillus acidophilus and lactobacillus plantarum are common strains in conventional Kimchi and Dairy fermentation,
Long-term use demonstrates their safety in practice.They are included in Microecological functional food by the Ministry of Public Health can
In the strain catalogue used.It is current research shows that, lactobacillus acidophilus can significantly reduce the level of Blood Cholesterol.Acidophilus breast
Bacillus, Lactobacillus casei and streptococcus thermophilus are proved can be by enhancing human natural killer cell activity, enteron aisle being stimulated to exempt from
Epidemic disease related target, effectively adjusts human immune system, these immunoregulation effects, plays and inhibits pathogenic microorganisms and prevention swollen
The effect of knurl.
It is found with gas chromatographic analysis lactobacillus acidophilus fermentating metabolism product, fermented supernatant fluid contains a large amount of lactic acid and certain
The butyric acid (or isobutyric acid) of amount, acetic acid, positive fumaric acid, six carbon fatty acids, trace amounts of propionic acid, succinic acid and micro antibacterial are more
Peptide.They have Gram-negative bacteria different degrees of inhibiting effect not only to gram-positive bacteria.Numerous studies show
The organic acid that lactic acid bacteria generates declines enteral pH and Eh, enteral acidic environment is caused, to pathogenic entero becteria such as shigella dysenteriae, sand
Door Salmonella, staphylococcus aureus, Escherichia coli, clostridium difficile, rotavirus have antagonism, assist human immunological competence.
Short chain fatty acids and lactic acid can inhibit the growth of putrefactivebacteria, so as to reduce the toxic amine of these substances generation, indole, Yin
Diindyl, ammonia, scatol, hydrogen sulfide, carcinogen and other poisonous substances mitigate liver detoxification burden significantly.Acetate, propionate and breast
Hydrochlorate may also reduce very low density lipoprotein and cholesterol in blood and play a role to fat, the Metabolism regulation of lactic acid bacteria.
In addition to this, lactic acid bacteria thalline (including dead thalline), fragment (peptide glycan mainly on cell wall) have to causing
The effect that the noxious substance of mutation combines so as to weaken and eliminate the toxic action of these substances, protects liver.Bacterium simultaneously
Body and cell wall fragment can also, stimulate enteron aisle target spot, enhance immunocompetence, adsorb harmful substance, therefore can effectively adjust immune work
With.
Cordycepin in Cordyceps militaris, can antiviral, antibacterial, significantly inhibition tumour growth, interference germ RNA and DNA synthesis.
Riched Cordyceps Militaris contains cordycepic acid, and cordycepic acid can effectively prophylactic treatment cerebral thrombus, cerebral hemorrhage, renal failure, while also have diuresis
Effect.Adenosine in Cordyceps militaris, antiviral, antibacterial, prophylactic treatment cerebral thrombus, cerebral hemorrhage inhibit Platelet congregation prevention thrombus
It is formed.Cordyceps sinensis polysaccharide in Cordyceps militaris is improved immunity, slows down aging, strengthens the body resistance to consolidate the constitution, cardioprotection, liver, the work of anti-convulsion
With.For Cordyceps militaris also rich in selenium, a large amount of scientific practice proves that selenium can significantly inhibit the growth of cancer cell, stimulates immune globulin
White and antibody generation, enhances body immunity.Cordycepin in Cordyceps militaris has antitumor, anti-aging, antibacterial, disease-resistant
Poison, immunological regulation improve metabolism, remove the multiple pharmacological effects such as free radical, can and liver cardiovascular with effective protection.
Multifunctional solid health food provided by the invention, can not only strengthen immunity function, moreover it is possible to prevent heart and brain blood
Pipe disease and protection liver function, are a kind of multi-functional health foods；Simultaneously using solid dosage (powder or capsule), make to disappear
Expense person, which carries, to take all easily.
Specific embodiment
It is further illustrated the present invention below in conjunction with specific embodiment, the present invention does not refer to that part is the prior art.
Embodiment 1
The preparation of Cordyceps militaris powder
(1) prepared by strain：The isolated Cordyceps militaris parent species COB201 from wild Chinese caterpillar fungus, access improvement PDA culture medium
In, 20 DEG C ± 1 DEG C, it is protected from light culture 10 days；It accesses in pedigree seed culture medium and is cultivated again, 20 DEG C ± 1 DEG C, shaking table culture 5 days；Training
The Cordceps militaris bacterium solution supported is put into refrigerator, spare.
Improve PDA culture medium formula (1000ml)：
Potato 200g, sucrose 200g, agar 200g, Jia Shui supply 1000ml.
Medium for original variety (1000ml)：
Potato 250g, sucrose 10g, peptone 1g, carrot 10g, onion 10g, mushroom 20g, egg 5g, alum 0.05g,
Sodium bicarbonate 1g, Jia Shui supply 1000ml.
(2) dispensing is bottled：Culture bottle volume is 265ml, adds in rice 24g in each culture bottle, water 32ml, after mixing on
Lid prepares sterilizing.
(3) it sterilizes：Culture bottle marshalling is put into autoclave, at 122 DEG C, high pressure steam sterilization 30 minutes is cold
But it after, takes out and prepares inoculation.
(4) it is inoculated with：By the cultured original seed liquid of step (1), connect by the original seed liquid that 2.5ml is added in each culture bottle
Kind.
(5) it cultivates：Cordceps militaris culture bottle after inoculation, moves in culturing rack, controls 20 DEG C ± 1 DEG C of cultivation temperature, humidity
Control is 85%, daily illumination 9 hours, intensity of illumination 1500lx-3500lx, daily ventilation 1 hour or more, cultivates 45 days,
It can be picked after fructification maturation.
(6) dry powder processed：Fruiting bodies of cordyceps militaris after picking under the conditions of 70 DEG C is dried 4 hours, is crushed to after taking-up
80 mesh.
The preparation of probiotics spray dried powder
(1) lactobacillus acidophilus, lactobacillus plantarum and Lactobacillus casei slant strains are routinely connect respectively by microbiological manipulations
In the seed culture medium through high pressure sterilization (0.1MPa, 20min), 37 DEG C are cultivated 12 hours kind, and primary seed solution is made；Three bacterium
Kind primary seed solution be inoculated with by volume for 1: 10 into the seed culture medium through high pressure sterilization (0.1MPa, 20min) respectively,
37 DEG C are cultivated 12 hours, and secondary seed solution is made；The secondary seed solution of three strains is using volume ratio as 1：2000 ratio is common
It accesses in the fermentation medium through high pressure sterilization (0.1MPa, 20min), 37 DEG C are cultivated 16 hours, and probiotics fermention liquid is made.
The seed culture medium, fermentation medium are according to mass percent meter by following material composition：
Soybean germ 2.5%, peanut germ 1.0%, lotus seeds 3.5%, jujube 1.5%, matrimony vine 0.4%, Poria cocos 0.6%,
Dusty yeast 0.3%, glucose 0.8%, soda ash 0.1%, surplus are water.
(2) after probiotics fermention liquid obtained being concentrated in vacuo to the 25% of original volume, concentrate is obtained, into concentrate
Microcrystalline cellulose is added in, 200g microcrystalline celluloses are added in every liter of concentrate, it is spray-dried to obtain probiotics spray dried powder；
Pressure during vacuum concentration is -0.1Mpa, and feed temperature is 83 DEG C；Inlet air temperature is 170 DEG C during spray drying, and leaving air temp is
70℃。
A kind of preparation method of multifunctional solid health food, includes the following steps：
Aforementioned 35 parts of the Cordyceps militaris powder being prepared, 65 parts of probiotics spray dried powder is taken to be mixed, mixed material warp
80 mesh are sieved, and then capsule is filling prepares capsule, and the multifunctional solid health food that specification is 0.3 gram/is made.
Embodiment 2
The preparation of Cordyceps militaris powder
(1) prepared by strain：The isolated Cordyceps militaris parent species COB201 from wild Chinese caterpillar fungus, access improvement PDA culture medium
In, 20 DEG C ± 1 DEG C, it is protected from light culture 10 days；It accesses in pedigree seed culture medium and is cultivated again, 20 DEG C ± 1 DEG C, shaking table culture 5 days；Training
The Cordceps militaris bacterium solution supported is put into refrigerator, spare.
Improve PDA culture medium formula (1000ml)：
Potato 200g, sucrose 200g, agar 200g, Jia Shui supply 1000ml.
Medium for original variety (1000ml)：
Potato 250g, sucrose 10g, peptone 1g, carrot 10g, onion 10g, mushroom 20g, egg 5g, alum 0.05g,
Sodium bicarbonate 1g, Jia Shui supply 1000ml.
(2) dispensing is bottled：Culture bottle volume is 265ml, adds in rice 24g in each culture bottle, water 32ml, after mixing on
Lid prepares sterilizing.
(3) it sterilizes：Culture bottle marshalling is put into autoclave, at 122 DEG C, high pressure steam sterilization 30 minutes is cold
But it after, takes out and prepares inoculation.
(4) it is inoculated with：By the cultured original seed liquid of step (1), connect by the original seed liquid that 2.5ml is added in each culture bottle
Kind.
(5) it cultivates：Cordceps militaris culture bottle after inoculation, moves in culturing rack, controls 20 DEG C ± 1 DEG C of cultivation temperature, humidity
Control is 85%, daily illumination 9 hours, intensity of illumination 1500lx-3500lx, daily ventilation 1 hour or more, cultivates 45 days,
It can be picked after fructification maturation.
(6) dry powder processed：Fruiting bodies of cordyceps militaris after picking under the conditions of 70 DEG C is dried 4 hours, is crushed to after taking-up
80 mesh.
The preparation of probiotics spray dried powder
(1) lactobacillus acidophilus, lactobacillus plantarum and Lactobacillus casei slant strains are routinely connect respectively by microbiological manipulations
In the seed culture medium through high pressure sterilization (0.1MPa, 20min), 37 DEG C are cultivated 14 hours kind, and primary seed solution is made；Three bacterium
Kind primary seed solution be inoculated with by volume for 1: 10 into the seed culture medium through high pressure sterilization (0.1MPa, 20min) respectively,
37 DEG C are cultivated 16 hours, and secondary seed solution is made；Lactobacillus plantarum, lactobacillus acidophilus secondary seed solution using volume ratio as 1：
2000 ratio, the secondary seed solution of Lactobacillus casei are using volume ratio as 1:1000 ratio is accessed through high pressure sterilization jointly
In the fermentation medium of (0.1MPa, 20min), 37 DEG C are cultivated 14 hours, and probiotics fermention liquid is made.
The seed culture medium, fermentation medium are according to mass percent meter by following material composition：
Soybean germ 3.0%, peanut germ 1.5%, lotus seeds 4.0%, jujube 1.0%, matrimony vine 0.6%, Poria cocos 0.4%,
Dusty yeast 0.1%, glucose 1.0%, soda ash 0.15%, surplus are water.
(2) after probiotics fermention liquid obtained being concentrated in vacuo to the 20% of original volume, concentrate is obtained, into concentrate
Microcrystalline cellulose is added in, 250g microcrystalline celluloses are added in every liter of concentrate, it is spray-dried to obtain probiotics spray dried powder；
Pressure during vacuum concentration is -0.08Mpa, and feed temperature is 73 DEG C；Inlet air temperature is 150 DEG C during spray drying, leaving air temp
It is 90 DEG C.
A kind of preparation method of multifunctional solid health food, includes the following steps：
Aforementioned 30 parts of the Cordyceps militaris powder being prepared, 70 parts of probiotics spray dried powder is taken to be mixed, mixed material warp
80 mesh are sieved, and then granule filling prepares powder, and multifunctional solid health food of the specification for 1 gram/packet is made.
Embodiment 3
The preparation of Cordyceps militaris powder
(1) prepared by strain：The isolated Cordyceps militaris parent species COB201 from wild Chinese caterpillar fungus, access improvement PDA culture medium
In, 20 DEG C ± 1 DEG C, it is protected from light culture 10 days；It accesses in pedigree seed culture medium and is cultivated again, 20 DEG C ± 1 DEG C, shaking table culture 5 days；Training
The Cordceps militaris bacterium solution supported is put into refrigerator, spare.
Improve PDA culture medium formula (1000ml)：
Potato 200g, sucrose 200g, agar 200g, Jia Shui supply 1000ml.
Medium for original variety (1000ml)：
Potato 250g, sucrose 10g, peptone 1g, carrot 10g, onion 10g, mushroom 20g, egg 5g, alum 0.05g,
Sodium bicarbonate 1g, Jia Shui supply 1000ml.
(2) dispensing is bottled：Culture bottle volume is 265ml, adds in rice 24g in each culture bottle, water 32ml, after mixing on
Lid prepares sterilizing.
(3) it sterilizes：Culture bottle marshalling is put into autoclave, at 122 DEG C, high pressure steam sterilization 30 minutes is cold
But it after, takes out and prepares inoculation.
(4) it is inoculated with：By the cultured original seed liquid of step (1), connect by the original seed liquid that 2.5ml is added in each culture bottle
Kind.
(5) it cultivates：Cordceps militaris culture bottle after inoculation, moves in culturing rack, controls 20 DEG C ± 1 DEG C of cultivation temperature, humidity
Control is 85%, daily illumination 9 hours, intensity of illumination 1500lx-3500lx, daily ventilation 1 hour or more, cultivates 45 days,
It can be picked after fructification maturation.
(6) dry powder processed：Fruiting bodies of cordyceps militaris after picking under the conditions of 70 DEG C is dried 4 hours, is crushed to after taking-up
80 mesh.
The preparation of probiotics spray dried powder
(1) lactobacillus acidophilus, lactobacillus plantarum and Lactobacillus casei slant strains are routinely connect respectively by microbiological manipulations
In the seed culture medium through high pressure sterilization (0.1MPa, 20min), 37 DEG C are cultivated 16 hours kind, and primary seed solution is made；Three bacterium
Kind primary seed solution be inoculated with by volume for 1: 10 into the seed culture medium through high pressure sterilization (0.1MPa, 20min) respectively,
37 DEG C are cultivated 14 hours, and secondary seed solution is made；Lactobacillus plantarum, lactobacillus acidophilus secondary seed solution using volume ratio as 1：
2000 ratio, the secondary seed solution of Lactobacillus casei are using volume ratio as 1:1000~2000 ratio jointly through high pressure go out by access
In the fermentation medium of bacterium (0.1MPa, 20min), 37 DEG C are cultivated 12 hours, and probiotics fermention liquid is made.
The seed culture medium, fermentation medium are according to mass percent meter by following material composition：
Soybean germ 2.5%, peanut germ 1.3%, lotus seeds 3.8%, jujube 1.3%, matrimony vine 0.5%, Poria cocos 0.5%,
Dusty yeast 0.2%, glucose 1.0%, soda ash 0.1%, surplus are water.
(2) after probiotics fermention liquid obtained being concentrated in vacuo to the 22% of original volume, concentrate is obtained, into concentrate
Microcrystalline cellulose is added in, 230g microcrystalline celluloses are added in every liter of concentrate, it is spray-dried to obtain probiotics spray dried powder；
Pressure during vacuum concentration is -0.09Mpa, and feed temperature is 80 DEG C；Inlet air temperature is 160 DEG C during spray drying, leaving air temp
It is 80 DEG C.
A kind of preparation method of multifunctional solid health food, includes the following steps：
Aforementioned 35 parts of the Cordyceps militaris powder being prepared, 65 parts of probiotics spray dried powder is taken to be mixed, mixed material warp
80 mesh are sieved, and then capsule is filling prepares capsule, and the multifunctional solid health food that specification is 0.8 gram/is made.
Functional study data, " sample " as described below are carried out using multifunctional solid health food made from embodiment 1
For multifunctional solid health food made from embodiment 1.
Functional study data：
The zoopery that the multifunctional solid health food of the present invention is cooked through ocean university, according to " function of health food
The criterion of assessment process and the method for inspection ", it was demonstrated that multifunctional solid health food of the invention has to chemically liver
Damage has auxiliary protection function and regulating blood lipid action.Specific experiment and judgment method are as follows：
(1) assistant protection function of chemically liver operation is tested
It is daily 3g/60kg according to human body recommended dose.This experiment sets basic, normal, high three dosage groups：0.25、0.5、
1.5g/kg is equivalent to 5,10,30 times of human body recommended dose, separately sets liver injury model group and Normal group (is given and distilled
Water).
Experimental animal：Cleaning grade Kun Ming mice, male, 18-22 grams
Experimental method and result：
Each dosage group gives sample, and control group and model group drink distilled water, continuous 28 days.28th day each dosage group and mould
Type group CCl4Contamination, dosage 24mg/kg are injected intraperitoneally (i.p).29th day, 5 groups of animals plucked eyeball and take blood, and centrifugation takes blood
Clearly.Measure following biochemical indicator：Serum glutamic pyruvic transminase (GPT), glutamic-oxalacetic transaminease (GOT), white/globulin (A/G)；And
Liver is taken to carry out histopathologic examination.Experimental result Spss software statistics.
1. weight
Influence of 1 sample of table to the weight of animals
As seen from the above table, each dosage group of sample compared with the control group, difference that there are no significant.
2. biochemistry detection result
2 biochemistry detection result of table
*P<0.05 and CCl4Model group compares, Analysis of variance
As seen from the above table, the glutamic-pyruvic transaminase of Normal group, glutamic-oxalacetic transaminease content are significantly lower than CCl4Model group is deposited
In significant difference, illustrate that model is set up.Glutamic-oxalacetic transaminease, the glutamic-pyruvic transaminase of sample high dose group are substantially reduced, with
CCl4 model groups compare, and there are significant differences.
3. pathologic finding：
3 Normal group pathologic finding of table integrates
Table 4CCl4 model groups pathologic finding integrates
5 low dose group pathologic finding of table integrates
6 middle dose group pathologic finding of table integrates
7 high dose group pathologic finding of table integrates
8 pathologic finding total mark of table
*P<0.05 and CCl4Model group compares (Analysis of variance)
By gross examination and microscopy result as it can be seen that the pathology integration of Normal group exists significantly lower than CCl4 model groups
Significant difference illustrates that model is set up.Sample high dose group and CCl4Model control group is compared, and pathology integration is decreased obviously, and is existed
Significant difference.
Conclusion：Animal orally administration sample multifunctional solid health food has after 28 days to hepatic injury caused by chemicals
Protective effect.
(2) regulating blood lipid action is tested
According to the daily 3g/60kg of human body recommended dose, this experiment sets basic, normal, high three dosage group, respectively 0.25,0.5,
1.5g/kg is equivalent to 5,10,30 times of human body recommended dose, and prevention hyperlipemia model control group gives distilled water.
Experimental animal：SD rats, male, 170-200 grams of weight
High nutrition feed high in fat：Add cholesterol 1g, cholate 0.5g, lard 10g, yolk powder 10g in per 100g basal feeds
Experimental method and result：
Rat 40 is taken, after being fed with 5 days with normal diet, weighs, takes tail blood, surveys serum triglyceride, cholesterol, highly dense
The basic value of lipoprotein is spent, rat is then randomly divided into four groups according to basic blood lipid level, every group 10, respectively sample
Basic, normal, high three dosage group and prevention hyperlipemia model control group, according to dosage give sample, while each group is fed with feeding high in fat
Material after continuously feeding 28 days, is weighed, and big rathole blood is taken to survey every blood lipids index.
Influence of 9 sample of table to the weight of animals
As seen from the above table, each dosage group animal weightening of sample is compared with model control group, without significant difference.
Influence of 10 sample of table to animal cholesterol
Influence of 11 sample of table to animal triglycerides
Influence of 12 sample of table to animal high-density lipoprotein
By table 10, table 11, table 12 as it can be seen that the last sample high dose group of experiment compared with model control group, cholesterol and glycerine
Three esters are decreased obviously, significant difference.
Influence of 13 sample of table to blood fat heave amplitude
By table 13 as it can be seen that the last sample high dose group cholesterol of experiment and triglycerides decline respectively compared with model control group
15.8% and 18.0%, high-density lipoprotein rises 0.03mol/L (1.2mg/dL).
Conclusion：After sample (multifunctional solid health food) is continuously fed 28 days, there is regulating blood lipid action to rat.
Claims (10)
1. a kind of multifunctional solid health food, which is characterized in that according to mass percent meter by following material composition：Cordyceps militaris
Powder 30~35%, probiotics spray dried powder 65~70%；
The probiotics spray dried powder is by lactobacillus plantarum seed liquor, Lactobacillus casei seed liquor and lactobacillus acidophilus seed
After mixed fermentation, gained zymotic fluid is spray-dried to be made liquid.
2. multifunctional solid health food according to claim 1, which is characterized in that the lactobacillus plantarum seed liquor,
Inoculum concentration when Lactobacillus casei seed liquor and lactobacillus acidophilus seed liquor mixed fermentation is by volume 1：1~2：1.
3. multifunctional solid health food according to claim 1 or 2, which is characterized in that the probiotics spray drying
Powder is prepared with the following method：
Lactobacillus plantarum, Lactobacillus casei and lactobacillus acidophilus are inoculated in respectively through in autoclaved seed culture medium, 37
DEG C culture 12~16 hours, be made primary seed solution；The primary seed solutions of three strains respectively by volume for 1: 10 be inoculated with into through
In autoclaved seed culture medium, 37 DEG C are cultivated 12~16 hours, and secondary seed solution is made；Lactobacillus plantarum, acidophilus breast bar
The secondary seed solution of bacterium is using volume ratio as 1：2000 ratio, the secondary seed solution of Lactobacillus casei are using volume ratio as 1:1000
Through in autoclaved fermentation medium, 37 DEG C are cultivated 12~16 hours, probiotics hair is made in access to~2000 ratio jointly
Zymotic fluid；
After probiotics fermention liquid obtained is concentrated in vacuo to the 20~25% of original volume, concentrate is obtained, concentrate is through spraying
It is dried to obtain probiotics spray dried powder.
4. multifunctional solid health food according to claim 3, which is characterized in that microcrystalline cellulose is added in into concentrate
Element adds in 200~250g microcrystalline celluloses in every liter of concentrate, spray-dried to obtain probiotics spray dried powder.
5. multifunctional solid health food according to claim 4, which is characterized in that pressure during vacuum concentration is -0.1
~-0.08Mpa, feed temperature are 73~83 DEG C.
6. multifunctional solid health food according to claim 5, which is characterized in that inlet air temperature is during spray drying
150~170 DEG C, leaving air temp is 70~90 DEG C.
7. multifunctional solid health food according to claim 3, which is characterized in that the seed culture medium, fermentation training
Base is supported according to mass percent meter by following material composition：
Soybean germ 2.5-3.0%, peanut germ 1.0-1.5%, lotus seeds 3.5-4.0%, jujube 1.0-1.5%, matrimony vine 0.4-
0.6%, Poria cocos 0.4-0.6%, dusty yeast 0.1-0.3%, glucose 0.8-1.2%, soda ash 0.1-0.15%, surplus are water.
8. the preparation method of the multifunctional solid health food described in any of the above-described claim, which is characterized in that including as follows
Step：
Cordyceps militaris powder and probiotics spray dried powder mixed material are sieved through 80 mesh, and capsule or particle filling are prepared using capsule is filling
Dress prepares powder.
9. the preparation method of multifunctional solid health food according to claim 8, which is characterized in that the capsule is filling
Measure is 0.3 gram, 0.5 gram, 0.8 gram, 1.0 grams.
10. the preparation method of multifunctional solid health food multifunctional solid health food according to claim 8, special
Sign is that the powder filling amount is 1 gram, 2 grams, 50 grams.
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