CN108244109A - Application of the methyl stearate in Meloidogyne incognita is prevented - Google Patents
Application of the methyl stearate in Meloidogyne incognita is prevented Download PDFInfo
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- CN108244109A CN108244109A CN201711492510.5A CN201711492510A CN108244109A CN 108244109 A CN108244109 A CN 108244109A CN 201711492510 A CN201711492510 A CN 201711492510A CN 108244109 A CN108244109 A CN 108244109A
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- methyl stearate
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Toxicology (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
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- Wood Science & Technology (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to application of the methyl stearate in Meloidogyne incognita is prevented.Present invention firstly discovers that methyl stearate infecting and breed with certain inhibiting effect, and can significantly inhibit the nematode number in root knot, pieces of an egg and the soil of Meloidogyne incognita simultaneously to Meloidogyne incognita.During application, methyl stearate is safe and nontoxic, will not be brought a negative impact after to the growth of crops, and can also play certain facilitation to the growth of crops when amount of application is 0.5~1mmol/kg.dry soil.
Description
Technical field
The present invention relates to the prevention and control fields of Meloidogyne incognita, and more particularly to methyl stearate is in prevention Meloidogyne incognita
In application.
Background technology
Nematode (Nematodes) is a kind of low invertebrate, and type is various, has a very wide distribution.Colonize in plant
It is internal, referred to as plant nematode.Root-knot nematode (Meloidogyne spp.) is the generation of omnivorousness plant nematode
Table, host range is wide, can infect more than 3000 kinds plants, while aggravate soil-borne disease to a certain extent, and such as damping-off is withered
Disease etc. brings serious loss to world agriculture, it is considered to be first of ten big damage to crops production parasitic nematodes.According to incomplete
Statistics, the annual whole world therefore caused by economic loss up to 125,000,000,000 dollars.At present in the world it has been reported that root-knot nematode have
More than 98 plant, and wherein Meloidogyne incognita can cause a series of problems of furnished equipments, and make a large amount of industrial crops underproduction.
Meloidogyne incognita J2 larvas have the ability for infecting plant, migrate to tip of a root elongation zone and are infected.Nematode is bored
After entering host root, induction host's root cell is formed increased, multinuclear, metabolic more vigorous and is held for nematode
The continuous giant cell for providing nutriment, i.e., the root knot visually seen, host's root system is caused, which to be damaged, leads to moisture and nutritional ingredient source
Source is constantly lost, so as to which host be made to grow impaired and yield decline.Meloidogyne incognita is punctured using lancet simultaneously posts
The physical injury that chief cell wall is formed provides invasion channel for other causal organisms, has aggravated crop droop, root rot, has stood
The generation of the soil-borne diseases such as blight.
Banana is the important industrial crops in China, and it is tropical that China's banana is mainly distributed on Guangdong, Guangxi, Hainan and Yunnan etc.
Subtropical area.The trend of steady-state growth is presented in China's banana harvest area and total output in recent years.And as banana field is answered
Kind of index increases, and banana root knot nematode disease generally occurs, and aggravates year by year, and incidence is generally 20% to 30%, serious to reach
More than 60%.Aggrieved root system fibrous root is few, and in dark brown, epidermis rots when serious, seriously affect banana root system to mineral element and
The absorption of moisture eventually leads to the decline of banana production and quality.
At present, based on chemical prevention, a large amount of uses kill line chemical agent in actual production for the prevention of banana root-knot nematode
It is middle to achieve the effect that effectively to prevent root-knot nematode, it is the control method generally used in banana production.But the change used at present
Learning nematode killing agent, there are the problems such as toxicity is big, environmental pollution is serious.With the enhancing of Public environmental attitude and the anti-medicine of nematode
The increase of property, the application of chemical nematicides are increasingly subject to limit.The environmental benefit of biological control is good, to human body, Environmental security,
It is the research hotspot of present non-environmental pollution control root-knot nematode.
Methyl stearate is not only one of important component of biodiesel, and for producing surfactant, lubricant
It with the preparation of other organic chemicals, is widely used in chemical field, is a kind of common fatty acid methyl ester, in various plants
It is identified in root system secretion, but has not yet to see the report that methyl stearate is useful for prevention Meloidogyne incognita.
Invention content
The present invention's is the application for proposing methyl stearate in Meloidogyne incognita is prevented first.
Preferably, specific application form is that before crop-planting, methyl stearate is applied in soil.It is moving
Application can reduce the chance that root-knot nematode infects crop before planting crop, and anti-root-knot nematode effect is more preferable.
Preferably, it in application, is applied in soil after the methyl stearate is prepared into preparation.Methyl stearate is not
Water is dissolved in, can better disperse after being prepared into preparation, preferably play a role in the soil.
Preferably, the methyl stearate is prepared into ethanol solution.It is molten through ethyl alcohol since methyl stearate is not soluble in water
Liquid dissolving after be applied in soil before long-term cropping, can with the mixing of soil evenly, after ethyl alcohol volatilization after again into row crop
Transplanting, can prevent ethyl alcohol from bringing injury to crop.
It is further preferred that the mass concentration of methyl stearate is 0.5mol/L-8.0mol/L in the ethanol solution.
Preferably, the amount of application of the methyl stearate is 0.5~8mmol/kg.dry soil.
Preferably, the amount of application of the methyl stearate is 0.5~1mmol/kg.dry soil.In above-mentioned dosage model
In enclosing, it not only can more effectively inhibit root-knot nematode, can also play certain facilitation to the growth of plant.
Preferably, the amount of application of the methyl stearate is 1.8~2.2mmol/kg.dry soil.In above-mentioned concentration model
In enclosing, the number of pieces of an egg, the number of nematode and root knot number can be efficiently reduced, and above-mentioned additive amount will not be to the life of plant
Length is brought a negative impact, and when the amount of nematode is larger, the additive amount of above-mentioned concentration can be used to effectively inhibit nematode.
It is another object of the present invention to protect the Meloidogyne incognita inhibitor containing methyl stearate.
Preferably, the active ingredient of the inhibitor is methyl stearate or methyl stearate and the group of other active ingredients
Close object.
It is further preferred that the inhibitor is for the ethanol solution containing methyl stearate.
Final object of the present invention is the method for Sustainable use methyl stearate prevention Meloidogyne incognita, including such as
Lower step：
1) methyl stearate is configured to the ethanol solution of a concentration of 0.5mol/L-8.0mol/L；
2) before proportion of crop planting, by the alcohol suspension according to methyl stearate amount of application be 0.5~8mmol/
Kg.dry soil are administered in soil.
Preferably, the amount of application of the methyl stearate is 0.5~1mmol/kg.dry soil or 1.8~2.2mmol/
kg.dry soil。
The present invention has the advantages that：
Present invention firstly discovers that methyl stearate infecting and breeding and make with certain inhibition to Meloidogyne incognita
With, and can significantly inhibit the nematode number in root knot, pieces of an egg and the soil of Meloidogyne incognita simultaneously.
Methyl stearate is safe and nontoxic, will not be brought a negative impact after to the growth of crops, and in amount of application
During for 0.5~1mmol/kg.dry soil can also certain facilitation be played to the growth of crops.
Description of the drawings
Influence of Fig. 1 various concentrations methyl stearate to banana plant height.
Influence of Fig. 2 various concentrations methyl stearate to banana overground part fresh weight.
Influence of Fig. 3 various concentrations methyl stearate to root knot.
Influence of Fig. 4 various concentrations methyl stearate to pieces of an egg
Influence of Fig. 5 various concentrations methyl stearate to soil nematodes.
Specific embodiment
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..
Experimental method used in following embodiments is conventional method unless otherwise specified.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
Embodiment 1
The present embodiment is related to the experiment of methyl stearate anti-system to Meloidogyne incognita.
1st, experiment material：
For studying object banana Williams B6 (Musa AAA Cavendish CV.Williams), tissue-cultured seedling is fragrant from Guangxi
Feng Zhong industry Co., Ltd.
Meloidogyne incognita (Meloidogyne incognita) (force is raised, Zheng Jingwu, quotient's break force Taxonomy of Meloidogyne and
Identification approach and progress Zhejiang Agriculture journals .2005,17:106-110, the public can obtain from China Agricultural University) pieces of an egg.
Methyl stearate (Methyl hexadecanoate, CAS：112-39-0) buy spontaneous work bioengineering (Shanghai)
Methyl stearate mother liquor is made with absolute ethyl alcohol dissolving in limited company.
2nd, experimental method：
The application of methyl stearate：Methyl stearate is dissolved with ethyl alcohol, its a concentration of 01mol/L is made to be applied to sterile soil
In, the amount of application of methyl stearate is 0.5mmol/kg.dry soil, after soil is dried, loaded in flowerpot (1kg dry ground/
Basin), setting 3 is parallel.
Banana planting：Tissue culture seedlings of bananas is moved to seedlings nursing plate to cultivate on vermiculite 35 days, a Yuan Shi nutrition was poured every 3 days
During which liquid suitably waters according to vermiculite dry and wet degree.
It is transplanted in the sterile soil for applying methyl stearate when banana seedlings length to 4 expansion leaves, transplants banana
It pours a Yuan Shi nutrient solution within every 3 days after seedling, during which pours appropriate amount of deionized water, per 1 banana seedlings of pot transplanting.
Nematode is inoculated with：After banana transplantation of seedlings 15 days, it is making a call to 4 depth in the range of banana basal part of stem 1cm-2cm
The aperture of 2cm or so is squeezed into two age Meloidogyne incognita suspension in aperture with liquid-transfering gun, then with a small amount of sterilizing grave
It firmly aperture and suitably waters, 1000 or so two age Root Knots ± ± nematodes is inoculated with per basin, inoculation nematode receives sample after 35 days.
3rd, experimental result
Measure banana growth indexes (plant height, overground part fresh weight etc.), while measure nematode index (root knot number, pieces of an egg number,
Soil line borer population etc.), further calculate its average value.
Embodiment 2
Compared with Example 1, difference lies in the application of methyl stearate is 1mmol/kg.dry soil.
Embodiment 3
Compared with Example 1, difference lies in the application of methyl stearate is 2mmol/kg.dry soil.
Embodiment 4
Compared with Example 1, difference lies in the application of methyl stearate is 4mmol/kg.dry soil.
Embodiment 5
Compared with Example 1, difference lies in the application of methyl stearate is 8mmol/kg.dry soil.
Comparative example 1
This comparative example is blank control, compared with Example 1, difference lies in, methyl stearate is not applied in the soil,
Directly Meloidogyne incognita is inoculated with after plantation banana seedlings.
Experimental example
The result of Examples 1 to 5 and comparative example 1 such as table 1：
Table 1
As can be seen from the above data, the methyl stearate of various concentration to the elongation of plant height have certain promotion
Effect.But have centainly to the increase of overground part strain fresh weight when the concentration of methyl stearate is more than 4mmol/kg.dry soil
Inhibiting effect.As methyl stearate a concentration of 0.5-1.0mmol/kg.dry soil in the soil, perfume (or spice) can be remarkably promoted
The growth of any of several broadleaf plants overground part, the plant height of banana increase 14.0-21.2% (such as Fig. 1), and overground part fresh weight increases 9.9-11.9%
(such as Fig. 2).
The methyl stearate of various concentration infecting and breeding and play certain inhibiting effect to Meloidogyne incognita.
Compared with blank control (comparative example 1), in the range of experimental concentration, it can substantially reduce Banana Root and fasten root knot number
Amount, that is, significantly suppress Meloidogyne incognita and banana infected, inhibiting rate reaches 36.6-43.6% (such as Fig. 3), inhibition
Significantly.The pieces of an egg number on root system is substantially reduced simultaneously, i.e., methyl stearate significantly inhibits Root Knot line in the concentration range
The breeding of worm, inhibiting rate reach 42.2-67.0% (such as Fig. 4), substantially reduce the formation of pieces of an egg.Nematode digital display work in soil subtracts
It is few, 34.4-50.5% is reached to the inhibiting rate of nematode, inhibition is apparent (such as Fig. 5).
As a concentration of 2mmol/kg.dry soil, the growth of the aerial part of plant is not promoted significantly, still
It is most preferable that it integrates insecticidal effect.
Although above having used general explanation, specific embodiment and experiment, the present invention is made to retouch in detail
It states, but on the basis of the present invention, it can be made some modifications or improvements, this is apparent to those skilled in the art
's.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to claimed
Range.
Claims (10)
1. application of the methyl stearate in Meloidogyne incognita is prevented.
2. application according to claim 1, which is characterized in that before crop-planting, methyl stearate is applied to soil
In.
3. application according to claim 2, which is characterized in that be applied to soil after the methyl stearate is prepared into preparation
In earth.
4. the application according to Claims 2 or 3, which is characterized in that the methyl stearate is prepared into ethanol solution, it is excellent
Choosing, the mass concentration of methyl stearate is 0.5mol/L-8.0mol/L in the ethanol solution.
5. according to claim 2~4 any one of them application, which is characterized in that the methyl stearate applying in the soil
Dosage is 0.5~8mmol/kg.dry soil.
6. application according to claim 5, which is characterized in that the amount of application of the methyl stearate is 0.5~1mmol/
Kg.dry soil or 1.8~2.2mmol/kg.dry soil.
7. a kind of Meloidogyne incognita inhibitor containing methyl stearate.
8. inhibitor according to claim 7, which is characterized in that the active ingredient of the inhibitor for methyl stearate or
Methyl stearate and the composition of other active ingredients.
9. inhibitor according to claim 7 or 8, which is characterized in that the inhibitor is the second containing methyl stearate
Alcoholic solution.
A kind of 10. method using methyl stearate prevention Meloidogyne incognita, which is characterized in that include the following steps：
1) methyl stearate is configured to the ethanol solution of a concentration of 0.5mol/L-8.0mol/L；
2) before proportion of crop planting, by the ethanol solution of methyl hexadecanoate according to the amount of application of methyl hexadecanoate for 0.5~
8mmol/kg.dry soil are administered in soil；Preferably, the amount of application of the methyl stearate is 0.5~1mmol/
Kg.dry soil or 1.8~2.2mmol/kg.dry soil.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711492510.5A CN108244109A (en) | 2017-12-30 | 2017-12-30 | Application of the methyl stearate in Meloidogyne incognita is prevented |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711492510.5A CN108244109A (en) | 2017-12-30 | 2017-12-30 | Application of the methyl stearate in Meloidogyne incognita is prevented |
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| CN108244109A true CN108244109A (en) | 2018-07-06 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711492510.5A Pending CN108244109A (en) | 2017-12-30 | 2017-12-30 | Application of the methyl stearate in Meloidogyne incognita is prevented |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109939095A (en) * | 2019-04-01 | 2019-06-28 | 广州中医药大学 | A kind of application of methyl stearate |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1649578A (en) * | 2002-03-04 | 2005-08-03 | 戴弗根斯公司 | Nematicidal fatty acid and fatty acid ester related compounds |
| CN101090630A (en) * | 2004-11-03 | 2007-12-19 | 纳幕尔杜邦公司 | Formulations containing insect repellent compounds |
-
2017
- 2017-12-30 CN CN201711492510.5A patent/CN108244109A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1649578A (en) * | 2002-03-04 | 2005-08-03 | 戴弗根斯公司 | Nematicidal fatty acid and fatty acid ester related compounds |
| CN101090630A (en) * | 2004-11-03 | 2007-12-19 | 纳幕尔杜邦公司 | Formulations containing insect repellent compounds |
Non-Patent Citations (1)
| Title |
|---|
| MARC STADLER等: "Fatty Acids and Other Compounds with Nematicidal Activity from Cultures of Basidiomycetes", 《PLANTA MEDICA》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109939095A (en) * | 2019-04-01 | 2019-06-28 | 广州中医药大学 | A kind of application of methyl stearate |
| CN109939095B (en) * | 2019-04-01 | 2021-08-27 | 广州中医药大学 | Application of methyl stearate |
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Application publication date: 20180706 |