CN108241075A - A kind of method that single molecule force spectroscopy test is carried out in gaseous environment - Google Patents
A kind of method that single molecule force spectroscopy test is carried out in gaseous environment Download PDFInfo
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- CN108241075A CN108241075A CN201810038474.3A CN201810038474A CN108241075A CN 108241075 A CN108241075 A CN 108241075A CN 201810038474 A CN201810038474 A CN 201810038474A CN 108241075 A CN108241075 A CN 108241075A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01Q—SCANNING-PROBE TECHNIQUES OR APPARATUS; APPLICATIONS OF SCANNING-PROBE TECHNIQUES, e.g. SCANNING PROBE MICROSCOPY [SPM]
- G01Q60/00—Particular types of SPM [Scanning Probe Microscopy] or microscopes; Essential components thereof
- G01Q60/24—AFM [Atomic Force Microscopy] or apparatus therefor, e.g. AFM probes
Abstract
A kind of method that single molecule force spectroscopy test is carried out in gaseous environment, belongs to single molecule force spectroscopy field of measuring technique.Solve the problems, such as how single molecule force spectroscopy detection is carried out in gaseous environment.The test method of the present invention, it is that first the substrate for carrying sample is placed on objective table, it is less than 20% that the AFM probe of atomic force microscope, which is adjusted, with the relative humidity of sample local environment, then it is moved by the flexible drive probe of scanner, power distance Curve is obtained finally by the parameter of laser detector and scanner, so as to obtain mechanical signal of the polymer molecule under gaseous environment.This method can cause the process of melting unwinding and the single-stranded behavior and mechanical response of mechanism and polymer molecule under gaseous environment in the single-stranded power of Study Polymer Melts platelet under gaseous environment, and this method between the tensile speed of polymer individual molecule chain can tens nanometers it is per second be adjusted to micron per minutes up to a hundred, the power precision of the power distance Curve of gained is 5 skin oxen.
Description
Technical field
The invention belongs to single molecule force spectroscopy field of measuring technique, and in particular to one kind carries out unimolecule power in gaseous environment
The method for composing test.
Background technology
Even to this day, polymer semicrystalline material is widely used in human society.Polymer semicrystalline material is by crystalline phase and without fixed
The internet of shape phase composition and form (Phys.Rev.Lett.2003,91,095502).There are a large amount of thickness in crystalline phase to receive
The other platelet of meter level, these platelets have polymer semicrystalline material mechanical property conclusive effect (Macromolecules
1999,32,4390-4403).It is high performance poly- for designing with molecular mechanism to understand molecular behavior of the platelet in stress and deformation
It is particularly significant to close object semicrystalline material.But platelet size is constrained to, the behavior of the individual molecule during stress and deformation is not easy
It is tracked and detects.The appearance of single molecule force spectroscopy technology causes above-mentioned idea to become possible.Single molecule force spectroscopy measuring method is
As the powerful tool for studying single polymer molecular chain behavior and property.The single-stranded mechanical property of Study Polymer Melts molecule,
Mechanical response behavior and intramolecular under stress have weight with intermolecular interaction to design new material from bottom to up
The directive significance (Nature 2010,465,69-73) wanted.
Single molecule force spectroscopy device mainly includes:Optical tweezer, magnetic tweezer and atomic force microscope (AFM) etc.
(Prog.Polym.Sci.2003,28,1271-1295).Optical tweezer is to unimolecule manipulate simultaneously measuring force using light probe
Spectrum；Magnetic tweezer is to unimolecule manipulate simultaneously measuring force spectrum using magnetic ball；Atomic force microscope is even by physical absorption, chemistry
Individual molecule is adhered to by connection or the method for specific binding to be measured on probe, and unimolecule is manipulated simultaneously by probe deformation
Measuring force is composed.The conventional single molecule force spectroscopy of optical tweezer, magnetic tweezer and atomic force microscope is carried out in liquid phase environment.But absolutely
The even most polymer materials of most of polymer semicrystalline material are used in gaseous environment.Polymer molecule exists
Liquid phase is from having different interface energy under gaseous environment.Different interfaces can most likely result in polymer molecule in varying environment
It is lower there are different structures and different mechanical response behaviors (J.Am.Chem.Soc.2010,132,6530-6540).In this way
Design is cannot be directly used in non-liquid ring in the single-stranded property of polymer, mechanical response behavior and the kinetic parameter that liquid phase obtains
The high-performance polymer material used in border.Therefore the application range of single molecule force spectroscopy receives restriction with meaning, have very big
Limitation.How single molecule force spectroscopy detection is carried out in gaseous environment into needing urgently to solve the problems, such as at this stage.
Invention content
The purpose of the present invention is to solve the single molecule force spectroscopy measuring methods in the prior art based on atomic force microscope
In deficiency, provide it is a kind of carried out in gaseous environment single molecule force spectroscopy test method.
The purpose of the present invention is what is be achieved through the following technical solutions：
The method that single molecule force spectroscopy test is carried out in gaseous environment, step are as follows：
Step 1: the substrate for carrying sample is placed on the objective table of atomic force microscope；
Step 2: it is less than 20% to adjust the AFM probe of atomic force microscope with the relative humidity of sample local environment；
Step 3: adjusting atomic force microscope is in power spectral model, the scanner of atomic force microscope is controlled to extend, driven
AFM probe approaches substrate with the constant rate of speed of 20nm/s~100 μm/s, and after the needle point of AFM probe touches substrate, AFM is visited
The needle point of needle is stopped with the permanent lower pressure of 1nN~10nN in substrate, and in holding process, needle point and the sample of AFM probe connect
It touches and forms bridged linkage, scanner is controlled to shrink later, drive AFM probe separate with the constant rate of speed of 20nm/s~100 μm/s
Substrate applies a constant force away from substrate on AFM probe, and scanner records AFM probe in the real-time of the direction of motion
Location parameter, the deformation quantity of laser detector detection AFM probe simultaneously provide real-time force value parameter；
The coefficient of elasticity of the AFM probe is more than 0.2N/m；
Step 4: power-distance Curve is obtained by the parameter of laser detector and scanner, so as to obtain polymer molecule
Mechanical signal under gaseous environment.
Preferably, it before step 3, further includes, adjusts atomic force microscope and be in imaging pattern, determine that sample institute is in place
It puts.
Preferably, step 5 is further included, whether the mechanical signal judged is unimolecule mechanical signal, if so,
Stop test, if it is not, repeating step 1~step 4.
Preferably, the atomic force microscope includes probe, quartz supports platform, AFM probe, glue cover and objective table；
Probe is arranged on the top of objective table；Quartz supports platform is fixed on the bottom of the scanner of probe；AFM probe passes through spring
Folder is fixed on the bottom of quartz supports platform, is moved by the flexible drive AFM probe of scanner perpendicular to the direction of objective table；
Glue cover is infundibulate, and both ends are equipped with opening, and osculum end cap is in the outer wall lower part of quartz supports platform, contact position sealing, big opening end
It is contacted with substrates seal, glue cover, quartz supports platform and substrate form the cavity of a closure；
The sample is placed in the cavity of closure, by the duct in quartz supports platform be passed through into cavity discharge gas with
Reach relative humidity requirement.
Preferably, the material of the glue cover is fluorubber or silica gel cushion rubber.
Preferably, the maximum moving distance of the scanner is 6 μm.
Preferably, the gas is nitrogen.
Preferably, the sample is that the Polymer Single Crystal obtained by weak solution from crystal seed method, weak solution spin coating form
Polymer film or dispersion polymer chain.
Preferably, in step 3, the residence time is 0.1s~3s.
Preferably, the needle point material of the AFM probe be silicon nitride, needle surface it is unmodified or be modified with amino/
Hydroxyl group.
Compared with prior art, beneficial effects of the present invention are：
1st, the method provided by the invention that single molecule force spectroscopy test is carried out in gaseous environment can be directly in gaseous environment
The behavior of lower Study Polymer Melts individual molecule chain and mechanism.
2nd, the apparatus structure that the method provided by the invention that single molecule force spectroscopy test is carried out in gaseous environment uses is simple,
It is easy to operate.
3rd, the method for single molecule force spectroscopy test provided by the invention that carried out in gaseous environment is to polymer individual molecule chain
Tensile speed can from tens nanometers it is per second regulated and controled to micron per minutes up to a hundred, most fast rate of extension compared with liquid phase power compose
Improve an order of magnitude.
4th, the power essence of power-distance Curve of the method provided by the invention that single molecule force spectroscopy test is carried out in gaseous environment
Spend for 5 skin oxen, hence it is evident that higher than 20 skin oxen of liquid phase power spectrum precision, be conducive to be promoted sensitivity that single molecule force spectroscopy measures with it is accurate
Property.
5th, the method provided by the invention that single molecule force spectroscopy test is carried out in gaseous environment can regulate and control Experimental Area
Microenvironment provides various test condition.
Description of the drawings
Fig. 1 is the schematic diagram that single molecule force spectroscopy test is carried out under gaseous environment of the present invention；
Fig. 2 is the AFM scan image of the PEO monocrystalline of the embodiment of the present invention 1；
Fig. 3 be the embodiment of the present invention 1 AFM probe in liquid phase and gaseous environment with the rate of 2 μm/s far from obtained by substrate
The typical PEO power arrived causes power-distance Curve of unwinding；
Fig. 4 for the embodiment of the present invention 1 AFM probe with the rate of 0.02 μm/s and the rate of 100 μm/s far from substrate institute
Obtained typical PEO power causes power-distance Curve of unwinding；
Fig. 5 be the embodiment of the present invention 1 AFM probe in liquid phase and gaseous environment with different rates far from substrate when obtained by
The power precision of power-distance Curve；
Fig. 6 be the embodiment of the present invention 1 AFM probe in gaseous environment with different rates far from the power obtained by substrate-away from
From curve；
Fig. 7 be the embodiment of the present invention 1 AFM probe in liquid phase environment with different rates far from the power obtained by substrate-away from
From curve；
Fig. 8 is exemplary force-distance Curve that the AFM probe of embodiment 2 pulls out single PS chains from PS ultrathin membranes；
Fig. 9 is the pass of the adherency distance and AFM probe coefficient of elasticity in AFM probe resulting force-distance Curve of embodiment 2
System's figure；
Figure 10 be embodiment 2 adhesion strength of the AFM probe under different microenvironments in resulting force-distance Curve with it is relatively wet
The relational graph of degree；
In figure, 1, probe, 11, scanner, 12, laser detector, 13, prism, 14, laser light source, 2, quartz supports
Platform, 3, AFM probe, 4, glue cover, 5, objective table, 6, sample, 7, substrate.
Specific embodiment
In order to further appreciate that the present invention, the preferred embodiments of the invention are retouched With reference to embodiment
It states, but it is to be understood that these descriptions are only for the feature and advantage that further illustrate the present invention rather than to patent of the present invention
It is required that limitation.In present embodiment, X, Y, Z-direction are vertical two-by-two, using horizontal direction as X-direction, using vertical direction as Z side
To, using perpendicular to paper direction as Y-direction.
As shown in Figure 1, the method that single molecule force spectroscopy test is carried out in gaseous environment of the present invention, step are as follows：
Step 1: the substrate for carrying sample 6 is placed on the objective table 5 of atomic force microscope；
Step 2: the relative humidity for adjusting AFM probe 3 and 6 local environment of sample of atomic force microscope be 20% hereinafter,
It is preferred that less than 10%；
Step 3: adjusting atomic force microscope is in power spectral model, the scanner 11 of atomic force microscope is controlled to extend, band
The needle point of dynamic AFM probe 3 approaches substrate 7 with the constant rate of speed of 20nm/s~100 μm/s, when the needle point of AFM probe 3 touches base
Behind bottom 7, the needle point of AFM probe 3 stops 0.1s~3s with the permanent lower pressure of 1nN~10nN in substrate 7, in holding process,
The needle point of AFM probe 3 contacts to form bridged linkage with sample 6, later control scanner 11 shrink, drive AFM probe 3 with
The constant rate of speed of 20nm/s~100 μm/s apply far from substrate 7 or on AFM probe the constant force of 10pN~1nN away from
Substrate 7 during this, carries out unimolecule stretching, and scanner 11 records real time position parameter of the AFM probe 3 in the direction of motion, swashs
Photodetector 12 detects the deformation quantity of AFM probe 3 and provides real-time force value parameter；
Wherein, the coefficient of elasticity of AFM probe 3 is more than 0.2N/m, generally use 0.2N/m~1.0N/m；
Step 4: power-distance Curve is obtained by the parameter of laser detector 12 and scanner 11, so as to obtain polymer
Mechanical signal of the molecule under gaseous environment.
In the present invention, it is poly- that sample 6 is that the Polymer Single Crystal obtained by weak solution from crystal seed method, weak solution spin coating form
Compound film or the polymer chain of dispersion；Substrate 5 is silicon base, silanization silicon base, hydroxylating silicon base, slide or mica
Piece；The fixed form of sample 6 and substrate is physical absorption, chemical crosslinking or specificity interact；Scanner 11 is piezoelectricity
Ceramic scanner；The needle point material of AFM probe 3 is silicon nitride, and needle surface is unmodified or is modified with amino/hydroxyl group.
It,, can be by adjusting atomic force before step 3 when the area that sample 6 loads on substrate 5 is smaller in the present invention
Microscope is in imaging pattern, to determine 6 position of sample.
In the present invention, to ensure that testing result is accurate unimolecule mechanical signal, step 5 is further included, judgement obtains power
Learn whether signal is unimolecule mechanical signal, if so, stopping test, if it is not, repeating step 1~step 4.Judgement side
Method is the prior art that those skilled in the art grasp, and the present invention provides several foundations, but not limited to this：
Firstth, it intuitively goes to judge from the clean level of probability and force curve for obtaining signal, if obtaining the general of signal
Rate is relatively low, signal is simpler, it is possible to prove the monomolecular stretching of the stretch behavior；
Secondth, molecule is not broken, to the operation that the molecule is back and forth stretched, the reciprocal generation of the molecule stretches and pine
It relaxes, observation stretches either whether relaxation curve can overlap and stretch or whether the corresponding force value of relaxation curve is consistent, such as
Fruit stretches can overlap with relaxation curve and force value is consistent, it is possible to prove the monomolecular stretching of the stretch behavior；
Third, normalized by being tested to end mark molecule stretching, obtain the tensile elongation of molecule on force curve
There is statistical distribution, just as spring, the coefficient of elasticity of the spring of material different length of the same race is the same, therefore to same
After (i.e. under a certain force value, tensile elongation normalization) is normalized in a kind of stretching of molecule, the stretching of same property
Force curve will coincide together, it is possible to tell the monomolecular stretching of stretch behavior；
4th, fitting parameter is fitted stress strain curve by freely-jointed chain model or worm chain model, obtains every
The fitting parameter (library grace length, segment coefficient of elasticity) of stress strain curve is all more close can to prove single point of the stretch behavior
The stretching of son；
5th, the statistical distribution of power according to the mechanical property of stretching molecule and force curve feature, carries out corresponding mechanics system
Meter, such as to platform force curve statistical platform force value, to unimodal or multimodal force curve statistics summit end force value etc..If obtained power
Distribution value is relatively narrow, then the stretch behavior is then monomolecular stretching.
In the present invention, realize that the mode that the relative humidity of AFM probe 3 and 6 local environment of sample is less than 20% can lead to
It overregulates laboratory overall humidity environment to realize, is that simple modifications are carried out to existing atomic force microscope preferably, is AFM
Probe 3 provides closed space with sample 6, which can also adjust other microenvironments in addition to it can adjust humidity；
The atomic force microscope includes probe 1, quartz supports platform 2, AFM probe 3, glue cover 4 and objective table 5；Wherein, it sweeps
First 1 top for being arranged on objective table 5 is retouched, probe 1 is the prior art, by scanner 11, laser detector 12, prism 13 and is swashed
Radiant 14 forms, and scanner 11 is used to AFM probe 3 be driven to move, while record AFM probe 3 in the direction of motion (Z-direction)
Real time position parameter；Laser detector 12 detects the deformation quantity of AFM probe 3 so as to provide real-time force value parameter, laser light source
The laser of 14 transmittings reflects successively through AFM probe 3 and prism 13, into laser detector 12；Quartz supports platform 2 is fixed on scanning
The bottom of first 1 scanner 11, quartz supports platform 2 are the prior art, are internally provided with duct；AFM probe 3 is the prior art, is led to
The bottom that spring clip is fixed on quartz supports platform 2 is crossed, by the flexible drive AFM probe 3 of scanner 11 perpendicular to objective table 5
Direction (Z-direction) it is mobile；Glue cover 4 is infundibulate, and both ends are equipped with opening, and osculum end cap is under the outer wall of quartz supports platform 2
Portion, contact position sealing, big opening end and substrate 7 are in sealing contact, and glue cover 4, quartz supports platform 2 and substrate 7 form the chamber of a closure
Body,
Sample 6 is placed in the cavity of closure, and discharge gas is passed through into cavity by the duct in quartz supports platform 2 to reach
To relative humidity requirement, gas is generally nitrogen；The material of glue cover 4 is fluorubber or silica gel cushion rubber；To avoid glue cover 4 and substrate
7 separation, the maximum moving distance of scanner 11 is within 6 μm.
With reference to embodiments and attached drawing further illustrates the present invention.Detection device employed in embodiment is above-mentioned letter
Single improved equipment.
The power of 1 polyoxyethylene monocrystalline of embodiment causes melting unwinding research
Sample 6 is polyoxyethylene (PEO) monocrystalline mutually obtained in solution from crystal seed method, and substrate 7 is silicon amide substrate, sample
Product 6 are adsorbed by physical action in substrate 7；The coefficient of elasticity of AFM probe 3 is 0.2N/m；The material of glue cover 4 is fluorubber；
Gas is passed through as nitrogen；Relative humidity is 11%.
Test process is：The substrate 7 for carrying sample 6 is arranged on objective table 5, passes through the imaging mould of atomic force microscope
Formula determines 6 position of sample, as shown in Fig. 2, then changing atomic force microscope into power spectral model, AFM probe 3 is with 20nm/s
The rate of~100 μm/s approaches substrate 7, and after being contacted with substrate 7,0.1s is stopped with the lower pressure of 1nN so that AFM probe 3 with
PEO molecule contacts form bridged linkage, and scanner 11 is shunk later so that AFM probe 3 is with the rate of 20nm/s~100 μm/s
Far from substrate 7, PEO molecular motions are driven so that the unwinding from crystal of PEO molecules.Pass through laser detector 12 and scanner 11
Parameter obtain power-distance Curve, so as to obtain the process and mechanism that the single-stranded power of polyoxyethylene monocrystalline causes melting unwinding.
Fig. 3 be the embodiment of the present invention 1 AFM probe in liquid phase and gaseous environment with the rate of 2 μm/s far from obtained by substrate
Exemplary force-the distance Curve arrived；Fig. 4 is the AFM probe of the embodiment of the present invention 1 with the rate of 0.02 μm/s and the speed of 100 μm/s
Rate causes power-distance Curve of unwinding far from the obtained exemplary force of substrate；Fig. 5 is the AFM probe of the embodiment of the present invention 1 in liquid phase
With gaseous environment with different rates far from substrate when resulting force-distance Curve power precision；Fig. 6 and Fig. 7 be respectively in gas phase and
In liquid phase environment, power-distance Curve that AFM probe is obtained with different rates far from substrate 7.In Fig. 3~7, rate is approached
It is identical with far from rate.From Fig. 3~7 as can be seen that the test method of the present invention can carry out unimolecule power in gaseous environment
Spectrum experiment, and to the tensile speed of polymer individual molecule chain can from tens nanometers it is per second to micron per minutes up to a hundred into
Row regulation and control, most fast rate of extension improve an order of magnitude compared with liquid phase；The power of the power-distance Curve essence that gas phase single molecule power spectrum obtains
It spends for 5 skin oxen, power precision is better than liquid phase resulting force-distance Curve with accuracy；And different microenvironments is required to unwinding
Force value and mechanism all have an impact；The present invention can regulate and control Experimental Area using the prior art by carrying out simple transformation to device
Microenvironment, various test condition is provided, it is easy to operate.
The research of single-stranded movement in 2 polystyrene condensed state of embodiment
Sample 6 is to prepare polystyrene (PS) ultrathin membrane in hydroxylating silicon base by the method for spin coating；AFM probe 3
Coefficient of elasticity is 0.02N/m~0.7N/m；The material of glue cover 4 is fluorubber；Gas is passed through as nitrogen；Relative humidity for 11%~
40%.
Test process is：It first and then by the substrate 7 for carrying sample 6 is placed on objective table 5, atomic force microscope is tuned into power
Spectral model is extended by scanner 11 and AFM probe 3 is driven to move so that and AFM probe 3 approaches substrate 7 with the rate of 2 μm/s, with
After substrate 7 contacts, 3s is stopped with the lower pressure of 10nN so that AFM probe 3 forms bridged linkage with PS molecule contacts, sweeps later
It retouches device 11 to shrink so that AFM probe 3 far from substrate 7, drives PS molecular motions with the rate of 2 μm/s so that PS molecules are from film
Middle unwinding.Power-distance Curve is obtained by the parameter of laser detector and scanner 11, so as to obtain Single Chain Polystyrene in nothing
The process of entire chain movement and the resistance being subject in the film of setting.
Fig. 8 is exemplary force-distance Curve that the AFM probe of the embodiment of the present invention 2 pulls out single PS chains from PS ultrathin membranes；
Fig. 9 is the relational graph of the adherency distance and AFM probe coefficient of elasticity in AFM probe resulting force-distance Curve of embodiment 2；Figure
10 be the relationship of adhesion strength and relative humidity of the AFM probe of embodiment 2 under different microenvironments in resulting force-distance Curve
Figure.Fig. 8 curve obtaineds be with the AFM probe that coefficient of elasticity is 0.2N/m when relative humidity is 20% obtained by；Fig. 9 is opposite
The experiment that humidity carries out when being 11%；Figure 10 is the experiment that the AFM probe for being 0.2N/m with coefficient of elasticity carries out.It can be with from Fig. 8
Find out, test method of the invention can carry out single molecule force spectroscopy experiment in gaseous environment；From fig. 9, it can be seen that with bullet
The increase of property coefficient, adherency distance reduce, and are conducive to the acquisition of experimental signal, when the coefficient of elasticity of AFM probe increases to
During 200pN/nm, adherency distance drops to 50nm hereinafter, having been influenced on experimental signal acquisition smaller；From fig. 10 it can be seen that
As humidity declines, adhesion strength declines, and when relative humidity is less than 20%, adhesion strength reaches 30nN hereinafter, being conducive to experiment letter
Number acquisition；From Fig. 8~10 it can be seen that the present invention can be regulated and controled using the prior art by carrying out simple transformation to device
The microenvironment of Experimental Area provides various test condition, easy to operate.
Liquid phase environment detection in attached drawing as a comparison, is that nitrogen is replaced with hexadecane as unitary variant, other are not
Become.
Obviously, the above embodiments are merely examples for clarifying the description, and is not intended to limit the embodiments.It is right
For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of variation or
It changes.There is no necessity and possibility to exhaust all the enbodiments.And the obvious variation thus extended out or
Among changing still in the protection domain of the invention.
Claims (10)
1. the method for single molecule force spectroscopy test is carried out in gaseous environment, which is characterized in that step is as follows：
Step 1: the substrate for carrying sample (6) is placed on the objective table (5) of atomic force microscope；
Step 2: it is less than 20% to adjust the AFM probe (3) of atomic force microscope with the relative humidity of sample (6) local environment；
Step 3: adjusting atomic force microscope is in power spectral model, the scanner (11) of atomic force microscope is controlled to extend, driven
AFM probe (3) approaches substrate (7) with the constant rate of speed of 20nm/s~100 μm/s, when the needle point of AFM probe (3) touches substrate
(7) after, the needle point of AFM probe (3) is stopped with the permanent lower pressure of 1nN~10nN in substrate (7), and in holding process, AFM is visited
The needle point of needle (3) contacts to form bridged linkage with sample (6), later control scanner (11) shrink, drive AFM probe (3) with
The constant rate of speed of 20nm/s~100 μm/s applies a constant force away from substrate far from substrate (7) or to AFM probe
(7), scanner (11) record AFM probe (3) is in the real time position parameter of the direction of motion, laser detector (12) detection AFM spies
The deformation quantity of needle (3) simultaneously provides real-time force value parameter；
The coefficient of elasticity of the AFM probe (3) is more than 0.2N/m；
Step 4: power-distance Curve is obtained by the parameter of laser detector (12) and scanner (11), so as to obtain polymer
Mechanical signal of the molecule under gaseous environment.
2. the method according to claim 1 that single molecule force spectroscopy test is carried out in gaseous environment, which is characterized in that step
It before three, further includes, adjusts atomic force microscope and be in imaging pattern, determine sample (6) position.
3. the method according to claim 1 that single molecule force spectroscopy test is carried out in gaseous environment, which is characterized in that also wrap
Step 5 is included, whether the mechanical signal judged is unimolecule mechanical signal, if so, stopping test, if it is not, repeating
Step 1~step 4.
4. the method according to claim 1 that single molecule force spectroscopy test is carried out in gaseous environment, which is characterized in that described
Atomic force microscope includes probe (1), quartz supports platform (2), AFM probe (3), glue cover (4) and objective table (5)；Probe
(1) it is arranged on the top of objective table (5)；Quartz supports platform (2) is fixed on the bottom of the scanner (11) of probe (1)；AFM is visited
Needle (3) is fixed on the bottom of quartz supports platform (2) by spring clip, is existed by the flexible drive AFM probe (3) of scanner (11)
It is moved perpendicular to the direction of objective table (5)；Glue cover (4) is infundibulate, and both ends are equipped with opening, and osculum end cap is in quartz supports platform
(2) outer wall lower part, contact position sealing, big opening end and substrate (7) are in sealing contact, glue cover (4), quartz supports platform (2) and substrate
(7) cavity of a closure is formed；
The sample (6) is placed in the cavity of closure, and discharge gas is passed through into cavity by the duct in quartz supports platform (2)
To reach relative humidity requirement.
5. the method according to claim 4 that single molecule force spectroscopy test is carried out in gaseous environment, which is characterized in that described
The material of glue cover (4) is fluorubber or silica gel cushion rubber.
6. the method according to claim 1 that single molecule force spectroscopy test is carried out in gaseous environment, which is characterized in that described
The maximum moving distance of scanner (11) is 6 μm.
7. the method according to claim 4 that single molecule force spectroscopy test is carried out in gaseous environment, which is characterized in that described
Gas is nitrogen.
8. the method that single molecule force spectroscopy test is carried out in gaseous environment according to claim 1~7 any one, special
Sign is, the sample (6) is the polymer that the Polymer Single Crystal obtained by weak solution from crystal seed method, weak solution spin coating form
Film or the polymer chain of dispersion.
9. the method that single molecule force spectroscopy test is carried out in gaseous environment according to claim 1~7 any one, special
Sign is, in step 3, the residence time is 0.1s~3s.
10. the method that single molecule force spectroscopy test is carried out in gaseous environment according to claim 1~7 any one,
It is characterized in that, the needle point material of the AFM probe (3) is silicon nitride, and needle surface is unmodified or is modified with amino/hydroxyl base
Group.
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| CN109444476A (en) * | 2018-10-15 | 2019-03-08 | 上海交通大学 | A kind of preparation method of atomic force microscope sub-micron probe |
| CN109489545A (en) * | 2018-11-13 | 2019-03-19 | 中国科学院上海应用物理研究所 | A kind of protein analytical methods based on nanometer mechanics |
| CN109557341A (en) * | 2018-11-22 | 2019-04-02 | 中国科学院上海应用物理研究所 | A kind of accurate high-throughput single molecule force spectroscopy method |
| CN111721504A (en) * | 2020-06-17 | 2020-09-29 | 中国科学院物理研究所 | Method for measuring and calculating height conversion coefficient of magnetic tweezers under low force and application |
| CN112946321A (en) * | 2021-01-30 | 2021-06-11 | 南京理工大学 | Method for quantifying monomolecular force between ionic liquid and solid interface |
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