CN108226555B

CN108226555B - Rapid sample adding device and method for clinical samples

Info

Publication number: CN108226555B
Application number: CN201810216797.7A
Authority: CN
Inventors: 李涛
Original assignee: Individual
Current assignee: Individual
Priority date: 2018-03-16
Filing date: 2018-03-16
Publication date: 2024-05-03
Anticipated expiration: 2038-03-16
Also published as: CN108226555A

Abstract

The application discloses a device and a method for rapidly adding samples to clinical specimens, which comprise the following steps: the application can realize the simultaneous sample adding of a plurality of holes of a plurality of even more than dozens of test templates, and the sample adding quantity is large enough for the test templates for clinical examination.

Description

Rapid sample adding device and method for clinical samples

Technical Field

The invention relates to the field of medical examination, in particular to a device and a method for rapidly adding samples to clinical specimens.

Background

In the fields of medical clinical detection and the like, the treatment process of clinical specimens is very complicated, and a large number of operations such as pipetting, sample adding and mixing are often carried out, so that the sample adding efficiency of the clinical specimens is very important in the process. FIG. 1 is a clinical specimen test template commonly used in the prior art, which is mostly used for bacteria identification and drug susceptibility identification experiments in the field of medical clinical examination. Fig. 2 is a partial enlarged view of a sample testing hole of the testing template, the sample loading amount of the testing template can only be controlled below 25 mu L, and the sample testing hole is horizontally arranged, so that the gas discharge is not facilitated, and the gas resistance is easily generated at the upper part. In addition, when the sample adding amount is required to be larger, the structure cannot well meet the use requirement, and the use limitation is limited and cannot be further expanded.

For example, in a mycoplasma drug sensitivity identification test, the sample loading amount of a clinical sample is required to be 100 mu L, the sample loading work can only be completed manually, usually a sample loading gun (or a pipette) is used, the samples required to be detected by staff every day are numerous, tens of holes on a test template corresponding to each sample need to be loaded one by one, and the workload is huge.

When mycoplasma detection or drug sensitivity test is carried out, partial human tissues or mucus tend to exist in the liquid to be detected, when sample addition is carried out, uneven tissue and mucus distribution is easy to occur, and the phenomenon of pipeline blockage or 'jumping holes' is caused.

Meanwhile, under the condition that energy cannot be supplied, the detection device becomes a stack of waste, normal work cannot be performed, and finally, detection personnel can only perform sample loading work by adopting an original method, so that the working efficiency is greatly influenced, and the improvement of the applicability of the device is also urgently needed.

Disclosure of Invention

The application mainly solves the technical problem of providing a device and a method for rapidly adding samples to clinical specimens, and by implementing the technical scheme of the application, the simultaneous sample adding to a plurality of holes of a plurality of even more than dozens of test templates can be realized, and the sample adding amount is enough. In order to solve the technical problems, the application adopts the following technical scheme:

A test template, comprising: the surface layer and the bottom layer are respectively attached to two opposite surfaces of the template, and a liquid storage tank, an auxiliary bin, an air passage, a sample testing hole and a liquid flow passage are arranged on the template; the template is communicated with one end of the connecting pipe through a liquid storage tank, and the liquid storage tank is communicated with the sample testing hole through a liquid flow channel; the auxiliary bin is arranged in pairs with the specimen testing holes, the auxiliary bin is communicated with the specimen testing holes through an air passage, the testing holes and the auxiliary bin are of through hole structures, the specimen testing holes are vertically and longitudinally arranged, the center line of the auxiliary bin and the center line of the specimen testing holes form an included angle of 0-90 degrees, one end of the connecting pipe is communicated with the liquid storage tank, and the other end of the connecting pipe is provided with an anti-blocking device.

Further, a bearing is arranged at the center of the bottom surface of the anti-blocking device, a rotating shaft penetrates through the bottom surface of the anti-blocking device through the bearing and is connected with the anti-blocking device, a tower-shaped helical blade is arranged on the rotating shaft in the anti-blocking device, the shape and the size of the tower-shaped helical blade are matched with the inside of the anti-blocking device, and a gap is reserved between the tower-shaped helical blade and the side wall and the bottom surface of the anti-blocking device; the rotating shaft exposed outside the anti-blocking device is provided with a paddle.

Furthermore, the anti-blocking device is in a conical bottle shape, filtering holes are arranged on the side wall and the bottom surface of the anti-blocking device in a penetrating way, the inner diameter of each filtering hole is smaller than the inner diameter of each branch runner, and the filtering holes are distributed on the side surface and the bottom surface of the anti-blocking device.

Further, the liquid flow channel comprises a main flow channel and a branch flow channel, and the main flow channel is communicated with the bottom of the specimen testing hole through the branch flow channel.

Further, a reserved cabin is further arranged on the template, the reserved cabin is arranged between the liquid storage tank and the liquid runner, and the communication position of the liquid runner and the reserved cabin is lower than the communication position of the connecting pipe and the liquid storage tank in the vertical direction.

A rapid sample loading device for clinical specimens with test templates, comprising: the test device comprises a closed cabin, a negative pressure pump, a pressure control valve, a test template, a connecting pipe, a tray and a specimen bottle, wherein the negative pressure pump and the pressure control valve are respectively arranged on the closed cabin and are communicated with the inner part of the closed cabin, two ends of the connecting pipe are respectively connected to the specimen bottle and the test template, test template slots are arranged on the tray at equal intervals side by side, a specimen bottle support is arranged at one end of each test template slot, a specimen bottle device is arranged on the specimen bottle support, the test template is inserted on the test template slots, and the tray device is arranged in the closed cabin.

Further, the airtight cabin includes cabin panel and hatch door, the inside post die cavity and the loading cabin of being provided with of airtight cabin, the hatch door sets up on the loading cabin, installs sealing device on the hatch door, the inside cylindrical cavity of post die cavity forms and extends the cabin, extend the inslot plug-in device and have the plug, the plug inserts the one end that extends the cabin and is provided with the chock plug, and the other end is connected with spherical universal joint, is connected with the lead screw on the spherical universal joint.

Further, the material of the plug is medical rubber, and the plug is in interference fit with the inner wall of the extension cabin.

Further, the deck plate is of a double-layer plate structure, and the bottom plate and the top plate form a main body structure of the deck plate.

The rapid sample adding method for clinical samples by using the rapid sample adding device for clinical samples comprises the following steps:

Step one: respectively filling a plurality of clinical calibration liquids to be tested into specimen bottles, and respectively arranging the specimen bottles in specimen bottle holders;

Step two: inserting the test template into the test template slot;

step three: each test template is connected with the corresponding specimen bottle through two ends of a connecting pipe;

Step four: the tray is integrally inserted and mounted on a bin bottom plate of the closed bin; the bottom edge of the closed cabin is cut with the sealing device and forms a closed cavity with the cabin bottom plate of the closed cabin;

Step five: closing the pressure control valve, opening the negative pressure pump, and sequentially enabling the auxiliary bin on the test template main body and the gas in the sample test hole to pass through the liquid flow channel, the liquid storage tank, the connecting pipe and generate bubbles in the clinical sample to be tested for discharge.

Step six: closing the negative pressure pump, controlling the air pressure in the closed cabin through the pressure control valve, controlling the sample adding quantity of the sample testing holes by combining the size of the auxiliary cabin, realizing the liquid adding operation of batch and large liquid adding quantity, and completing the sample adding after the sample adding quantity is reached in a plurality of testing holes.

Further, the surface layer and the bottom layer are transparent film layers, and the surface layer and the bottom layer are arranged as transparent chambers with the test holes and the auxiliary bin.

Further, the main runner is arranged on the front face of the template.

Further, the branch flow channel is arranged on the back surface of the template.

Further, a through hole is formed at the joint of the main runner and the branch runner.

Further, the angle between the blade and the horizontal is 0 DEG to 5 deg.

Further, the lower end of the rotating shaft of the anti-blocking device is connected with a weight through a flexible connecting piece.

Further, a placement plane with the width not less than half of the width of the closed cabin is arranged between the closed cabin and the placement plane.

Further, the screw rod penetrates through a screw hole formed in the supporting piece to be connected with the handle, the screw rod is in threaded fit with the screw hole in the supporting piece, and the supporting piece is fixedly connected with the closed cabin.

Further, the outer surface of the plug is uniformly surrounded with one or a plurality of rings of sliding blocks, and the sliding blocks are provided with groove bodies in a penetrating way.

Further, the part of the support piece penetrating through the sliding block groove body is of a straight polish rod and other structures, and a layer of elastic damping gasket is arranged in the sliding block groove body.

Further, the screw hole is replaced by an overrunning clutch with internal threads.

Further, the top layer of cabin board includes axle, flabellum, two flabellums, the axle runs through flabellum and two flabellums to fix flabellum and two flabellums on the lateral wall of cabin board, make flabellum and two flabellums form the roof of cabin board, the flabellum sets up between two flabellums, and the quantity of flabellum and two flabellum is greater than 1, the axle is connected with drive mechanism, and drive mechanism drives the axle and carries out 90 rotations, and then flabellum and two flabellums and axle linkage make flabellum and two flabellums switch under horizontality and vertical state.

Further, ultraviolet lamps are arranged in the space between the top surface and the bottom surface of the deck plate, and the number of the ultraviolet lamps is more than or equal to 1.

Further, when the number is more than 1, the ultraviolet lamps are staggered.

Further, the ultraviolet lamp is also staggered with the shaft.

Further, the plate body constituting the sealed cabin is replaced by a cabin panel.

Further, the transmission device is driven by the motor to be linked with the shaft.

Further, the motor can be connected with a trigger switch, when the cabin door is in a closed state, the proximity switch is closed, the motor works to drive the shaft, the fan blades and the double fan blades to rotate by 90 degrees, and the ultraviolet lamp starts to work after the fan blades and the double fan blades are in a vertical state.

Further, the trigger switch is a proximity switch.

The beneficial effects of the invention include:

(1) By implementing the technical scheme of the application, the simultaneous sample addition of a plurality of holes of a plurality of even more than dozens of test templates can be realized, and the sample addition amount is large enough.

(2) The invention obviously reduces the labor intensity of operators, improves the working efficiency by more than tens of times, and greatly saves the treatment time of clinical specimens.

(3) The method saves manual operation and solves the problems of 'jumping holes', channel blockage and the like caused by uneven distribution of human tissues, body fluid and other components in the sample liquid to be detected.

(4) When the sample is added, the speed that the sample liquid enters the detection plate is high, so that the sample liquid is easy to mix with the air remained in the detection plate to generate bubbles, and finally, the detection hole cannot be filled with enough sample liquid or the sample liquid is blocked by the bubbles, so that the detection effect is influenced.

(5) When energy cannot be supplied, the device with the emergency manual sample adding function is provided, so that the applicability is higher, and emergency use can be performed in response to emergency situations.

(6) Can realize self-cleaning after the application of sample device carries out application of sample work, can reduce the artifical loss of clean link like this, make the device in time clear up, reduced the error rate of monitoring.

Drawings

The accompanying drawings are included to provide a further understanding of the disclosure, and are incorporated in and constitute a part of this specification, illustrate the disclosure and together with the description serve to explain, but do not limit the disclosure.

FIG. 1 is a schematic diagram of one embodiment of a test template provided by the present application;

FIG. 2 is a schematic perspective view of the rapid sample loading device for clinical specimens provided by the application;

FIG. 3 is a schematic view of a front and back side of one embodiment of a test template according to the present application;

FIG. 4 is a schematic structural view of another embodiment of the rapid sample loading device for clinical specimens provided by the application;

FIG. 5 is a schematic side cross-sectional view of a test template provided by the present application;

FIG. 6 is a schematic structural view of a rapid sample loading device for clinical specimens according to another embodiment of the present application;

FIG. 7 is a schematic view of the structure of a test template with anti-blocking device in example 2 provided by the present application;

FIG. 8 is a schematic structural view of an anti-blocking device in embodiment 2 provided by the present application;

FIG. 9 is a schematic view showing the structure of a capsule in embodiment 3 according to the present application;

FIG. 10 is a schematic view showing the structure of a deck panel in embodiment 4 according to the present application;

FIG. 11 is a view showing the use of the deck panel in the loading state according to example 4 provided by the present application;

fig. 12 is a view showing a use state of the deck panel in a sterilized state in embodiment 4 according to the present application.

Reference numerals illustrate:

1. Testing a template; 2. a specimen bottle; 3. a connecting pipe; 31. an anti-blocking device; 311. a filter hole; 312. a tower-shaped helical blade; 313. a rotating shaft; 314. a paddle; 4. a closed cabin; 41. a column cavity; 42. a sample adding cabin; 421. a cabin door; 43. an extension cabin; 431. a support; 432. a screw hole; 44. a plug; 441. a plug head; 442. a ball joint; 443. connecting a screw rod; 444. a handle; 445. a slide block; 45. a deck panel; 451. a shaft; 452. a fan blade; 453. double fan blades; 5. a liquid storage tank; 6. an auxiliary bin; 7. an airway; 8. a sample test hole; 9. a liquid flow channel; 10. a main flow passage; 11. a branch flow passage; 14. a tray; 15. a negative pressure pump; 16. a pressure control valve; 17. a clinical specimen to be tested; 18. a through hole; 20. a surface layer; 21. a bottom layer; 22. a template; 23. a specimen bottle holder; 24. testing the template slot; 25. sealing means, 26. Reserved compartment.

Detailed Description

The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings so that the advantages and features of the present invention can be more easily understood by those skilled in the art, thereby making clear and defining the scope of the present invention.

Example 1

As shown in fig. 1, 2,4 and 5, a rapid sample loading device for clinical samples comprises: the test device comprises a closed cabin 4, a negative pressure pump 15, a pressure control valve 16, a test template 1, a connecting pipe 3, a tray 14 and a specimen bottle 2, wherein the negative pressure pump 15 and the pressure control valve 16 are respectively arranged on the closed cabin 4 and are communicated with the interior of the closed cabin 4, two ends of the connecting pipe 3 are respectively connected to the specimen bottle 2 and the test template 1, the tray 14 is provided with test template slots 24 in parallel at equal intervals, one end of the test template slot 24 is provided with a specimen bottle support 23, the specimen bottle 2 is arranged on the specimen bottle support 23, the test template 1 is inserted on the test template slots 24, the tray 14 is arranged in the closed cabin 4, the test template 1 comprises a surface layer 20, a bottom layer 21 and a template 22, the surface layer 20 and the bottom layer 21 are respectively attached to two opposite surfaces of the template 22, and a liquid storage tank 5, an auxiliary bin 6, an air flue 7, a test hole 8 and a liquid flow channel 9 are arranged on the template; one end of the connecting pipe 3 is communicated with the liquid storage tank 5, and the liquid storage tank is communicated with the sample testing hole 8 through the liquid flow channel 9; the auxiliary bin 6 and the specimen testing holes 8 are arranged in pairs, the auxiliary bin 6 is communicated with the specimen testing holes 8 through the air passages 7, the testing holes 8 and the auxiliary bin 6 are of through hole structures, the surface layer 20 and the bottom layer 21 are transparent film layers, and the surface layer 20 and the bottom layer 21 are arranged into transparent cavities with the testing holes 8 and the auxiliary bin 6. The two surfaces of the test template 1 are covered with transparent films, and a transparent cavity is formed by the transparent films, the test holes 8 and the auxiliary bin 6. The specimen testing hole 8 is vertically and longitudinally arranged, and the center line of the auxiliary bin 6 and the center line of the specimen testing hole 8 form an included angle of 0-90 degrees.

The liquid runner 9 includes sprue 10 and branch runner 11, and sprue 10 communicates in sample test hole 8 bottom through branch runner 11, and sprue 10 sets up in template 22's front, and branch runner 11 sets up at template 22 back, and sprue 10 and each branch runner 11 junction is provided with through-hole 18, through setting up through-hole 18, has increased the right angle, has just also increased the retardant barrier of "backward flow" to the problem of backward flow has been solved.

In addition, more specimen testing wells 8 can be expanded as needed.

In another embodiment of the invention, as shown in fig. 6, the capsule 4 may be designed as a cuboid, and a door provided with a sealing device is arranged on the side of the capsule.

As shown in fig. 4, in a further embodiment of the present invention, the enclosure 4 is of a cylindrical design (benefit), the tray 14 is of a circular design, the edge of the bottom plate of the enclosure is provided with a sealing device 25, and the bottom edge of the enclosure 4 is engaged with the sealing device 25 and forms a closed chamber with the bottom plate of the enclosure.

The application method of the rapid sample adding device for the clinical samples is as follows:

The clinical specimen 17 to be tested is arranged in a specimen bottle 2, a test template 1 is inserted into a test template slot 24, the specimen bottles 2 are respectively arranged in a specimen bottle support 23, one end of a connecting pipe 3 is inserted into the specimen bottle 2, the other end of the connecting pipe is connected to the side wall of the test template 1 and communicated with a liquid storage tank 5 on the test template 1, the surface layer and the bottom layer of the test template 1 are plastic films, and a tray 14 is integrally inserted into a bin bottom plate of a closed cabin; the airtight cabin 4 and the cabin bottom plate of the airtight cabin form an airtight cavity through an airtight device 25, when the pressure of the airtight cabin 4 is reduced under the condition of human intervention (a pressure control valve 16 is closed, a negative pressure pump 15 is started), gas in an auxiliary cabin 6 and a specimen testing hole 8 on the testing template 1 sequentially passes through a liquid flow channel 9, a liquid storage tank 5, a connecting pipe 3 and bubbles are generated in a tested clinical specimen 17 to be discharged; when the closed cabin 4 recovers normal pressure under human intervention (the negative pressure pump 15 is closed, the pressure control valve 16 is opened), the clinical specimen 17 to be tested flows into each specimen testing hole 8 sequentially through the connecting pipe 3, the liquid storage tank 5 and the liquid flow channel 9 under the pressure effect, the amount of the sample added in each specimen testing hole 8 can be controlled by controlling the external pressure and combining the manufacturing size of the auxiliary cabin 6, after the sample addition is finished, the plastic pipe is cut off and sealed, and the testing template can perform the next detection work.

Based on the working principle of the technical scheme, a plurality of tested samples are added to a plurality of test hole sites of a plurality of corresponding test templates at one time through the change of the environmental pressure by human intervention, an auxiliary bin is arranged beside the sample test hole site, the middle of the auxiliary bin is connected with an air passage, and the size of the sample test hole 8 of the test template is set according to the requirement; the monitoring of mycoplasma requires a large amount of specimen liquid, the liquid amount is 100 mu L or more, and each sample adding of the specimen testing hole 8 is in a full state, so that the rapid generation of the specimen is facilitated; the filling amount of the sample in the sample testing hole 8 can be realized by adjusting the pressure control valve 16 to control the negative pressure in the closed cabin, and finally the filling amount of the testing template 1 is in an adjustable state; the size of the auxiliary bin 6 and the power of the negative pressure pump 15 can be determined according to the needs, so long as the full filling of the specimen testing hole can be ensured each time of sample adding; examples: sample is added to a sample test hole 8 with the volume of 100 mu L, if the volume of an auxiliary bin is set to 62 mu L, negative pressure reaches-0.054 to-0.056 MPa, the external pressure of a test board is controllable at any time, the sample adding amount of a test hole site is controlled by controlling the external pressure, and the sample adding amount can be 100 mu L or more under the action of the auxiliary bin.

According to the invention, the auxiliary bin is arranged on the test template, and the clinical specimen is rapidly loaded at the plurality of hole sites of the test template through the artificial intervention of the change of the environmental pressure and the size of the auxiliary bin, so that the loading quantity is enough to meet the use requirement, and the problems of high labor intensity and low working efficiency of staff are well solved.

Example 2

As shown in fig. 7 and 8, the scheme in this embodiment can be used in combination with embodiment 1, and the difference between the scheme in embodiment 1 and the scheme in embodiment 1 is that: the test template 1 is also provided with a reserved cabin 26, the reserved cabin 26 is arranged between the liquid storage tank 5 and the liquid flow channel 9, the volume of the reserved cabin 26 is larger than that of the liquid storage tank 5, and the communication position of the liquid flow channel 9 and the reserved cabin 26 is lower than the communication position of the connecting pipe 3 and the liquid storage tank 5 in the vertical direction.

The arrangement is to prevent that the sample liquid flows into the test template 1 at the moment of sample addition, and then is mixed with the residual air to generate foam due to the fast flow of the sample liquid, if the foam flows into the sample test hole 8 along with the sample liquid, the hole jumping phenomenon is easy to generate, if the hole jumping occurs, the subsequent detection steps are adversely affected, the detection result is distorted, and the diagnosis result of a doctor is further affected.

The reserving cabin 26 is arranged between the liquid storage tank 5 and the liquid flow channel 9, so that the foam mixed by the sample liquid and air is reserved in the reserving cabin 26 before the sample liquid flows into the test template 1, and the foam is suspended above the liquid level in the reserving cabin 26 because the foam is lighter than the sample liquid, and meanwhile, the liquid flow channel 9 is arranged at a position lower than the position where the connecting pipe 3 is communicated with the liquid storage tank 5, so that the foam is further prevented from entering the liquid flow channel 9, the occurrence of the jumping hole condition is reduced or even avoided, and the guarantee is provided for the accurate detection work.

When the reserved cabin 26 is used on the test board together with the test hole with the auxiliary cabin in embodiment 1, the hole jumping situation caused by the air bubbles is substantially improved, the reserved cabin 26 reduces the probability of the air bubbles entering the test board, the air bubbles can remain in the auxiliary cabin even if entering the test board, and meanwhile, the center line of the auxiliary cabin 6 forms an included angle of 0-90 degrees with the center line of the specimen test hole 8, so that the sample liquid with the air bubbles is difficult to flow back into the specimen test hole 8 in a short time after entering the auxiliary cabin, and the inventor does not find out the hole jumping phenomenon caused by the air bubbles through the combined use of the three technical characteristics.

Example 3

Meanwhile, in the process of sampling the mycoplasma sample solution, the problem of blockage of the sampling device is often caused, because the mycoplasma sample solution often comprises some human tissues or body fluids, once the tissues or body fluids are not sufficiently crushed or deposited in the sample bottle 2, blockage is easy to occur, so that sampling is failed, in order to solve the problem, the anti-blocking device 31 is additionally arranged at one end of the connecting pipe 3 extending into the sample bottle 2, the anti-blocking device 31 is in a conical bottle shape, the filtering holes 311 are arranged on the side wall and the bottom surface of the anti-blocking device 31 in a penetrating way, the inner diameter of the filtering holes 311 is smaller than the inner diameter of the branch passages 11, and the arrangement mode of the filtering holes 311 should be spread on the side surface and the bottom surface of the anti-blocking device 31.

Further, a bearing is arranged at the central position of the bottom surface of the anti-blocking device 31 and penetrates through the bottom surface of the anti-blocking device 31, a rotating shaft 313 is connected with the anti-blocking device 31 through the bearing, the rotating shaft 313 penetrates through the bottom surface of the anti-blocking device 31, a tower-shaped helical blade 312 is arranged on the rotating shaft 313 arranged at the inner section of the anti-blocking device 31, the size of the tower-shaped helical blade 312 is matched with the inside of the anti-blocking device 31, and a gap is reserved between the tower-shaped helical blade 312 and the side wall and the bottom surface of the anti-blocking device 31; the rotating shaft 313 exposed outside the anti-blocking device 31 is provided with a paddle 314, and the included angle between the paddle 314 and the horizontal is 0 degrees to 5 degrees.

The angle between the paddle 314 and the horizontal can be adjusted according to the clinical specimen 17 to be measured, if the clinical specimen 17 to be measured has more tissue or body fluid components and is easy to precipitate and agglomerate, the angle should be adjusted to 5 degrees, if the precipitation is not obvious, the angle of the paddle 314 can be adjusted to 0 degree to ensure the sample adding speed.

In the process of sample loading, the anti-blocking device 31 is arranged at one end of the connecting pipe 3 extending into the sample bottle 2 and is immersed into the clinical sample 17 to be tested, in the stage of negative pressure exhaust, the gas in the test template 1 is discharged through the connecting pipe 3 and then through the anti-blocking device 31, at the moment, due to the circulation of the gas, the tower-shaped helical blades 312 arranged in the anti-blocking device 31 rotate along with the rotation of the connecting pipe, and the further blades 314 also arranged on the rotating shaft 313 are linked with the tower-shaped helical blades 312, so that the anti-blocking device 31 can tilt, swing and the like, and the anti-blocking device 31 has the advantages of not only preventing blocking during sample loading, but also preventing bubbles from being hung on the anti-blocking device 31 in the stage of exhaust, facilitating gas discharge, and being stirred at the moment due to the rotation of the blades 314.

After the exhaust, the clinical specimen 17 to be tested enters the test template 1 under the action of air pressure, at this moment, the tower-shaped helical blade 312 in the anti-blocking device 31 starts to rotate under the action of air pressure, meanwhile, the rotating shaft 313 and the blade 314 are driven to rotate together, at this moment, the rotating action of the blade 314 can produce stirring effect on the clinical specimen 17 to be tested, under the reaction force of the clinical specimen 17 to the anti-blocking device 31, the anti-blocking device 31 can produce actions such as more severe inclination and swing than the exhaust stage, the clinical specimen 17 to be tested can be stirred through the actions, and meanwhile, tissues or body fluid attached to the anti-blocking device 31 can have more opportunities to enter the test template 1.

When the anti-blocking device 31 of the above type is adopted in actual measurement, the device weight is 6-8g (the weight of the connecting pipe 3 is not contained), the anti-blocking device 31 performs circular motion in the sample adding process, the circular motion forms a circular section, and when the position of the anti-blocking device 31 when the sample adding action is not started is taken as the initial position, the central axis of the anti-blocking device 31 at the initial position penetrates through the circle center of the circular section. For the first detection, the sample concentration is fixed, the texture is uniform, the sample is screened to facilitate the observation of the motion track of the anti-blocking device 31, the particle size of solid matters in the sample is less than 3/4 of the aperture of the filtering hole 311, the sample adding process lasts for 2-3 seconds, and the anti-blocking device 31 moves according to the motion mode in the process.

When the anti-blocking device 31 moves circumferentially along the cup wall below the liquid surface of the sample, wall collision sounds intermittently occur in the movement process, and the preliminary judgment is that the centrifugal force of the anti-blocking device 31 during movement is intermittently changed due to errors in the precision of injection molding and assembly. During the sample loading process, the sample is stirred together with the connecting pipe 3 by the anti-blocking device 31, but the sample does not splash, sprinkle and the like.

In the second actual measurement, other conditions are not changed, and a weight can be connected to the lower end of the rotating shaft 313 of the anti-blocking device 31, namely the liquid inlet end, through a flexible connecting piece, and meanwhile, the sample adopted in the experiment is not sieved, so that the solid material particle size is uneven. The rotation radius of the anti-blocking device 31 in the sample adding process is smaller than that in the previous experiment, and meanwhile, the anti-blocking device 31 is in the condition of the smaller rotation radius, and the sample amount in the test board reaches the standard after the sample adding process is finished. The weight of the test plate after the first and the second actual measurement is 115% and 113% of that of the traditional test plate (automatic sample addition), the jump porosity of the two test plates is 0%, and the amount of the sample in each test hole meets the standard required by the test.

In addition to the above two groups of actual measurements, the inventors conducted experiments of other groups, each of which had a porosity of 0%.

Example 4

As shown in fig. 9, the scheme in this embodiment may be used in combination with embodiment 1 or embodiment 2 or embodiment 3, and the difference between the scheme in reference to embodiment 1 or embodiment 2 or embodiment 3 and the scheme in embodiment 1, embodiment 2 or embodiment 3 is that: the capsule 4 includes: the sample loading cabin 42, the extension cabin 43 and the cabin door 421, wherein the cabin door 421 is arranged on one surface of the sample loading cabin 42, and the cabin door 421 is provided with a sealing device, so that the condition that the cabin door 421 is not tightly sealed when pressure change occurs in the closed cabin 4 is prevented, and the outside of the closed cabin 4 can be in various shapes for convenient use, but it should be noted that at least a placing plane which is not less than half the width of the closed cabin 4 is arranged between the closed cabin 4 and the placing plane, so that the closed cabin 4 can be placed stably in either a standby state or a using state.

The inside of the closed cabin 4 is of a cavity structure, a rectangular cavity is selected for illustration, a part of the rectangular cavity of the closed cabin 4 is a sample adding cabin 42, column cavities 41 are arranged in the other rectangular cavities, the appearance of the column cavities 41 is in interference fit with the rectangular cavities, an extension cabin 43 is formed by the cylindrical cavity in the column cavities 41, and the sample adding cabin 42 is of a rectangular space for conveniently placing the detection device.

The plug 44 is inserted into the extension cabin 43, one end of the plug 44 inserted into the extension cabin 43 is provided with a plug 441, the other end of the plug 44 is connected with a ball joint 442, the ball joint 442 is connected with a screw rod 443, the plug 44 is made of materials with smaller friction coefficient, such as hard plastics, toughened glass, metal and the like, the plug 441 is made of medical rubber, the plug 441 is in interference fit with the extension cabin 43, the screw rod 443 penetrates through a screw hole 432 arranged on the support member 431 to be connected with the handle 444, the screw rod 443 is in threaded fit with the screw hole 432 on the support member 431, and the support member 431 is fixedly connected with the closed cabin 4.

In use, a user holds the handle 444 to rotate, the handle 444 drives the screw 443 to rotate, the screw 443 drives the ball joint 442 to move relatively with the support 431, and the ball joint 442 drives the plunger 44 and the plug 441 in sequence to move along the extension cabin 43.

In order to prevent the force generated by the user turning the handle 444 from affecting the unstable matching position of the screw rod and the support member 431, it is also necessary to uniformly encircle the outer surface of the plug 44 with one or more circles of sliding blocks 445, and the sliding blocks 445 are provided with through grooves, so that the support member 431 can pass through the grooves and perform relative movement such as rail matching in the grooves, thus ensuring that the device will not affect the smooth operation of the device due to the radial force applied by the user, and the grooves on the sliding blocks 445 can help the plug 44 to perform smooth movement along the direction of the support member 431 and the extension cabin 43.

The part of the supporting member 431 penetrating through the groove body of the sliding block 445 can be of a straight polish rod and other structures, and meanwhile, a layer of elastic damping gasket can be added in the groove body of the sliding block 445, so that better anti-collision and stable movement effects can be achieved.

Further, the screw hole 432 can be replaced by an overrunning clutch with internal threads, so that the phenomenon that the device is reversed due to the fact that a user cannot grip the handle 444 or cannot grip the handle 444 when rotating the handle 444 is prevented, accidents happen, after the overrunning clutch is used, the device cannot be actively reversed, and the device can be reversed after external force is applied by the user, so that the use safety of the user is guaranteed, and meanwhile the device is more convenient.

The embodiment can be applied to the situation that no external energy source is supplied, a user opens the cabin door, and then the test template 1 is placed in the sample loading cabin together with other devices to close the cabin door;

Rotating the handle 444 to move the plug 441 in the extension compartment 43, causing the plug 441 to move outwardly from within the extension compartment 43; at this time, the sealed cabin 4 is used as a sealed cabin body, and the plug 441 and the extension cabin 43 are in interference fit, so that gas cannot enter the sealed cabin 4, under the condition that the gas quality is unchanged, the volume inside the sealed cabin 4 is enlarged, so that the air pressure inside the sealed cabin 4 is reduced from an initial state, at this time, the air pressure value inside the sealed cabin 4 is smaller than the air pressure value inside the test template 1, the gas inside the test template 1 flows from high pressure to low pressure, namely flows out of the test template 1, the handle 444 is reversed after the gas flows out, the volume inside the sealed cabin 4 is reduced, the air pressure is increased, and at this time, the air pressure after the rising can press the to-be-tested liquid into the test template 1, so as to finish the sample loading work.

Example 5

As shown in fig. 10, 11 and 12, the technical solution provided in this embodiment can refer to and refer to embodiment 1 or embodiment 2 or embodiment 3 or embodiment 4, unlike the solutions in embodiments 1, 2, 3 and 4 in that, in order to solve the problem that cleaning work is difficult to perform in the sealed cabin 4, and at the same time, cleaning personnel are exposed to contaminated environment when performing the cleaning work, the cabin body panel of the sealed cabin 4 is set to the cabin panel 45 as shown in fig. 10, 11 and 12, the cabin panel 45 is a double-layer plate structure, and the bottom plate and the top plate constitute the main body structure of the cabin panel 45.

The roof of cabin board 45 comprises axle 451, flabellum 452, double flabellum 453, and axle 451 runs through flabellum 452 and double flabellum 453 to fix flabellum 452 and double flabellum 453 on the lateral wall of cabin board 45, make flabellum 452 and double flabellum 453 constitute the roof of cabin board 45, flabellum 452 set up between two double flabellum 453, and the quantity of flabellum 452 and double flabellum 453 is greater than 1, and wherein axle 451 is connected with drive mechanism, and drive mechanism drives axle 451 and carries out 90 rotations, and then flabellum 452 and double flabellum 453 and axle 451 linkage make flabellum 452 and double flabellum 453 switch under horizontal state and vertical state.

Ultraviolet lamps are arranged in the space between the top plate and the bottom plate of the cabin panel 45, the number of the ultraviolet lamps can be multiple, the ultraviolet lamps are preferably arranged in a staggered mode, and further the ultraviolet lamps can be arranged in a staggered mode with the shaft 451.

The deck plate 45 may replace all the plate structures of the capsule 4.

The transmission device is driven by the motor to be linked with the shaft 451, and the motor needs to be a low-power and low-torque motor, so that the motor can be linked with the shaft 451, the fan blades 452 and the double fan blades 453.

The motor can be further connected with a trigger switch, such as a proximity switch, when the cabin door 421 is in a closed state, the proximity switch is closed, the motor works to drive the shaft 451, the fan blade 452 and the double fan blade 453 to rotate 90 degrees, and after the fan blade 452 and the double fan blade 453 are in a vertical state, the ultraviolet lamp starts to work, and at the moment, the ultraviolet lamp can perform disinfection work in the sealed cabin 4.

When test template 1 and other sample application devices are placed in sealed cabin 4, these devices can be pressed on the top plate of cabin panel 45, because the motor adopts a low-power and low-torque motor, fan 452 or double fan 453 can not rotate under the action of gravity, at this time, as long as one fan 452 or double fan 453 is blocked and can not rotate, the transmission device can not transmit, other fan 452 or double fan 453 can not rotate, the ultraviolet lamp can not work, and the phenomenon that sample liquid is overturned due to the fact that sealed cabin 4 is under the condition of the sample application device to be applied is avoided, and at this time, sealed cabin 4 can normally finish sample application work.

It is necessary to say here that the means for achieving sterilization are not limited to ultraviolet lamps, but can also be electric heating means or other means with mycoplasma killing function.

It should be noted here that the term "closed" in the name of the capsule 4 means that the capsule 4 is in a closed state during use.

The foregoing description is only illustrative of the present invention and is not intended to limit the scope of the invention, and all equivalent structures or equivalent processes or direct or indirect application in other related technical fields are included in the scope of the present invention.

Claims

1. A rapid sample adding device for clinical specimens, which is characterized in that: the test template (1) comprises a surface layer (20), a bottom layer (21) and a template (22), wherein the surface layer (20) and the bottom layer (21) are respectively attached to two opposite surfaces of the template (22), a reserved cabin (26), a liquid storage groove (5), an auxiliary cabin (6), an air channel (7), a specimen testing hole (8) and a liquid flow channel (9) are arranged on the template (22), the template (22) is communicated with one end of a connecting pipe (3) through the liquid storage groove (5), the liquid storage groove (5) is communicated with the specimen testing hole (8) through the liquid flow channel (9), the liquid flow channel (9) comprises a main flow channel (10) and a branch flow channel (11), the main flow channel (10) is communicated with the bottom of the specimen testing hole (8) through the branch flow channel (11), the reserved cabin (26) is arranged between the liquid storage groove (5) and the liquid flow channel (9), the communicating position of the liquid flow channel (9) and the reserved cabin (26) is lower than the communicating position of the liquid storage groove (3) and the liquid flow channel (5) in the vertical direction, the auxiliary cabin (6) is communicated with the specimen testing hole (8) through the auxiliary testing hole (8) and the two sets of the specimen testing holes (8), sample test hole (8) and auxiliary bin (6) are the through-hole structure, sample test hole (8) are vertical setting, the central line of auxiliary bin (6) is 0 ~ 90 contained angles with sample test hole central line, connecting pipe (3) one end and reservoir (5) intercommunication, and the other end is provided with anti-blocking device (31), anti-blocking device (31) are the erlenmeyer flask form, link up on the lateral wall and the bottom surface of anti-blocking device (31) and be provided with filtration pore (311), filtration pore (311) internal diameter is less than the internal diameter of branch road (11), filtration pore (311) are spread over on the side and the bottom surface of anti-blocking device (31), the bottom surface central point position of anti-blocking device (31) is provided with the bearing, and pivot (313) are run through the bottom surface of anti-blocking device (31) and are connected with anti-blocking device (31), set up be provided with tower form screw blade (312) on pivot (313) of anti-blocking device (31) inside section, the size of tower form screw blade (312) and the inside adaptation of device (31), and tower form screw blade (314) have on the lateral wall (314) and anti-blocking device (314) are the horizontal clearance that is between anti-blocking device (31) and anti-blocking device (314) and the top surface (314).

2. The rapid sample addition device for clinical samples according to claim 1, wherein: it still includes capsule (4), negative pressure pump (15), pressure control valve (16), test template (1), connecting pipe (3), tray (14) and sample bottle (2), negative pressure pump (15) and pressure control valve (16) are installed respectively on capsule (4) to with capsule (4) inside intercommunication, the both ends of connecting pipe (3) are connected respectively on sample bottle (2) and test template (1), equidistant side by side is provided with test template slot (24) on tray (14), is provided with sample bottle support (23) in test template slot's one end, and sample bottle (2) device is on sample bottle support (23), and test template (1) cartridge is on test template slot (24), and tray (14) device is in capsule (4).

3. The rapid sample addition device for clinical samples according to claim 2, wherein: the sealed cabin (4) comprises a cabin panel (45) and a cabin door (421), a column-shaped cavity (41) and a sample adding cabin (42) are arranged in the sealed cabin (4), the cabin door (421) is arranged on the sample adding cabin (42), a sealing device is arranged on the cabin door (421), a cylindrical cavity in the column-shaped cavity (41) forms an extension cabin (43), a plug (44) is arranged in the extension cabin (43), one end of the plug (44) inserted into the extension cabin (43) is provided with a plug head (441), the other end of the plug (44) is connected with a spherical universal joint (442), a screw rod (443) is connected to the spherical universal joint (442), and one circle or a plurality of circles of sliding blocks (445) are uniformly arranged on the outer surface of the plug (44).

4. The rapid clinical specimen loading device according to claim 3, wherein: the plug (441) is made of medical rubber, and the plug (441) is in interference fit with the inner wall of the extension cabin (43).

5. The rapid clinical specimen loading device according to claim 3, wherein: the deck plate (45) is of a double-layer plate structure, and the bottom plate and the top plate form a main body structure of the deck plate (45).

6. A rapid sample adding method for clinical samples is characterized in that: the method for performing rapid sample addition using the rapid sample addition device for clinical specimens according to claim 2, comprising the steps of:

Step two: inserting the test template into the test template slot;

Step five: closing a pressure control valve, opening a negative pressure pump, and sequentially enabling an auxiliary bin on the main body of the test template and gas in a sample test hole to pass through a liquid flow channel, a liquid storage tank, a connecting pipe and generate bubbles in a clinical sample to be tested to be discharged;