CN108210998A - It is a kind of to improve the water-soluble processing method of electrostatic spinning gelatin film and application - Google Patents
It is a kind of to improve the water-soluble processing method of electrostatic spinning gelatin film and application Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/60—Materials for use in artificial skin
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/222—Gelatin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0015—Electro-spinning characterised by the initial state of the material
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- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
- D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
- D04H1/728—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
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- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M11/00—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with inorganic substances or complexes thereof; Such treatment combined with mechanical treatment, e.g. mercerising
- D06M11/07—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with inorganic substances or complexes thereof; Such treatment combined with mechanical treatment, e.g. mercerising with halogens; with halogen acids or salts thereof; with oxides or oxyacids of halogens or salts thereof
- D06M11/30—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with inorganic substances or complexes thereof; Such treatment combined with mechanical treatment, e.g. mercerising with halogens; with halogen acids or salts thereof; with oxides or oxyacids of halogens or salts thereof with oxides of halogens, oxyacids of halogens or their salts, e.g. with perchlorates
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- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/16—Materials or treatment for tissue regeneration for reconstruction of eye parts, e.g. intraocular lens, cornea
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- D06M2101/00—Chemical constitution of the fibres, threads, yarns, fabrics or fibrous goods made from such materials, to be treated
- D06M2101/02—Natural fibres, other than mineral fibres
- D06M2101/10—Animal fibres
- D06M2101/14—Collagen fibres
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Abstract
The present invention discloses a kind of water-soluble processing method of raising electrostatic spinning gelatin film and application, the method are：1）Gelatin solution is placed in syringe, electrostatic spinning gelatin film is collected by method of electrostatic spinning；2）By the electrostatic spinning gelatin film with ClO 2 solution together be placed in sealing container, pass through volatility chlorine dioxide steam treatment gelatin film, treated, and gelatin film makes gelatin film superficial silicon dioxide chlorine volatilize completely by dry, ventilation process, then it is cleaned with deionized water, dry, the Static Spinning gelatin film handled using the above method can effectively be improved its water solubility and have good bioactivity.Processing method of the present invention is easy to operate, chlorine dioxide is cheap, and convenient for utilization and extention, and treated gelatin film is remained without chlorine element, so as to which with good bio-compatibility and application prospect, the requirement of the timbering materials such as organization engineering skin, cornea can be met.
Description
Technical field
The present invention relates to field of tissue engineering technology, be more particularly to a kind of water-soluble processing method of raising gelatin materials and its
Using.
Background technology
Gelatin has been obtained for being widely applied in field of tissue engineering technology, in all timbering materials, Static Spinning gelatin
Film due to good bioactivity, class extracellular matrix, biodegradable occupy consequence.However, due to Static Spinning
The water solubility of silk gelatin film is very poor to greatly limit its practicability as timbering material.Therefore, Static Spinning gelatin how is improved
The water solubility of film has become the hot issue of numerous workers' research.
Existing most of water-soluble method of gelatin film that improves reduces its bioactivity, is unfavorable for as timbering material
It uses.Seek a kind of easy, effectively, the processing method that can be improved the water solubility of gelatin film while retain its bioactivity has
Important application prospect.
Invention content
It is an object of the invention to a kind of water-soluble processing method of raising electrostatic spinning gelatin film and applications, utilize the party
The gelatin film of method processing can effectively improve its water solubility and have good bioactivity.
To achieve these goals, the present invention uses following technical scheme：
A kind of raising water-soluble processing method of electrostatic spinning gelatin film, includes the following steps：
1) gelatin solution of 25-30%w/v is placed in syringe, electrostatic spinning gelatin film is collected by method of electrostatic spinning；
2) 10- in sealing container will be placed in together with the electrostatic spinning gelatin film and the ClO 2 solution of 0.8-1.2g/L
14h, by volatility chlorine dioxide steam treatment gelatin film, treated, and gelatin film is placed in drying box dry 8-12h, then
Ventilation process 18-24h makes gelatin film superficial silicon dioxide chlorine volatilize completely, is then cleaned 2-3 times with deionized water, dry.
The preparation method of institute's gelatine solution is as follows：Gelatin is dissolved in NaCl solution, the magnetic agitation at 50-60 DEG C
18-24h obtains homogeneous gelatin solution.
The gelatin is animal bone gelatin or animal skin gelatin.
The molecular weight of the gelatin is 20-180KD.
The electrostatic spinning process, electrostatic spinning voltage are 14-18KV, and syringe needle is 13-17cm away from receiver distance, is promoted
The propulsion flow rate of pump is 0.28-0.32ml/h.
Collected electrostatic spinning gelatin film thickness is 0.45-0.55mm, and spinning is 150-200 nanometers a diameter of.
The preparation method of the ClO 2 solution is as follows：By K2S2O2It adds in deionized water solution, stirs to completely molten
Xie Hou adds NaClO2And be uniformly mixing to obtain ClO 2 solution, be subsequently placed in 4 DEG C of environment seal it is spare.Into one
Step, the K2S2O2And NaClO2Mass ratio be 1:1.
Gelatin is dissolved in sodium chloride solution, sodium chloride can improve solution and lead by the present invention first using above technical scheme
Electrically, conducive to the uniform nanoparticles gelatin film of diameter is subsequently prepared, the bright of class extracellular matrix is then prepared by method of electrostatic spinning
Gelatin copolymer solution under high pressure effect is stretched the film of formation of nanostructured, then passes through chlorine dioxide treatment electrostatic by glued membrane
Spinning gelatin film, ClO 2 solution have volatile performance, electrostatic spinning gelatin film are avoided to be in direct contact aqueous solution of chlorine dioxide
And moment dissolves, and can effectively improve its water solubility and be remained without chlorine element.
The present invention remarkable advantage be：(1) electrostatic spinning gelatin film has the nanostructured of class extracellular matrix, passes through two
Chlorine monoxid steam treatment electrostatic spinning gelatin film, method is simple, and operability is strong, can effectively improve its water solubility.(2) after handling
Gelatin film without chlorine element remain, have good bioactivity, can effectively degrade in vivo and can improve cell adherence, increase
It grows, the timbering materials such as skin, cornea can be used as to be applied to tissue repair.
Description of the drawings
Fig. 1 is SEM morphology analysis：A, the untreated electrostatic spinning gelatin films of B, C, D chlorine dioxide steam treatment 12h's
Electrostatic spinning gelatin film；
Fig. 2 is FTIR infrared analysis：The untreated Static Spinning gelatin films of A, the electrostatic of B chlorine dioxide steam treatments 12h
Spin gelatin film；
Fig. 3 is Mechanical Property Analysis：The electrostatic spinning gelatin film of A chlorine dioxide steam treatments 12h, B are untreated quiet
Electrospun gelatin film；
Fig. 4 is analyzed for swelling behavior：Gels be untreated electrostatic spinning gelatin film, crosslinkinggels bis-
Chlorine monoxid treated electrostatic spinning gelatin film；
Fig. 5 is MTT analyzing film bio-toxicities：The untreated electrostatic spinning gelatin films of A, B chlorine dioxide steam treatments 12h
Static Spinning gelatin film；
Fig. 6 is proliferated SEM figures for cell adherence on chlorine dioxide treatment caudacoria：A1 (=50 μm), A2 (=10 μm), A3 (=5
μm) it is one day；(=5 μm) of B1 (=50 μm), B2 (=10 μm), B3 is three days；C1 (=50 μm), C2 (=10 μm), C3 (=5 μ
M) it is 5 days；(=5 μm) of D1 (=50 μm), D2 (=10 μm), D3 is 10 days.
Specific embodiment
Several specific examples for the present invention below further describe the present invention, but invention is not limited only to this.
A kind of raising water-soluble processing method of electrostatic spinning gelatin film, includes the following steps：
1) gelatin is dissolved in NaCl solution, the magnetic agitation 18-24h at 50-60 DEG C obtains the homogeneous of 25-30%w/v
Gelatin solution；Wherein, the gelatin is animal bone gelatin or animal skin gelatin, and the molecular weight of the gelatin is 20-180KD；
Then the gelatin solution of 25-30%w/v is placed in syringe, electrostatic spinning gelatin is collected by method of electrostatic spinning
Film；Electrostatic spinning process, electrostatic spinning voltage are 14-18KV, and syringe needle is 13-17cm away from receiver distance, promotes the propulsion of pump
Flow rate is 0.28-0.32ml/h；Collected electrostatic spinning gelatin film thickness is 0.45-0.55mm, a diameter of 150-200 of spinning
Nanometer；
2) by K2S2O2It adds in deionized water solution, stirs to after being completely dissolved, add the NaClO of equivalent2, stirring
Uniformly obtain 0.8-1.2g/L ClO 2 solutions；
By above-mentioned electrostatic spinning gelatin film with ClO 2 solution together be placed in 10-14h in sealing container, pass through volatilization
Property chlorine dioxide steam treatment gelatin film, treated gelatin film is placed in drying box dry 8-12h, then ventilation process 18-
Gelatin film superficial silicon dioxide chlorine is made to volatilize completely for 24 hours, is then cleaned 2-3 times with deionized water, it is dry.
Embodiment 1
A kind of raising water-soluble processing method of electrostatic spinning gelatin film
1) animal bone gelatin is dissolved in NaCl solution, magnetic agitation for 24 hours, obtains the homogeneous bright of 25%w/v at 50 DEG C
Sol solution；Then gelatin solution is placed in syringe, electrostatic spinning gelatin film is collected by method of electrostatic spinning；Electrostatic spinning mistake
Journey, electrostatic spinning voltage are 14KV, and syringe needle is 13cm away from receiver distance, and the propulsion flow rate for promoting pump is 0.28ml/h；
2) by K2S2O2It adds in deionized water solution, stirs to after being completely dissolved, add the NaClO of equivalent2, stirring
Uniformly obtain 0.8g/L ClO 2 solutions；
By above-mentioned electrostatic spinning gelatin film with ClO 2 solution together be placed in 10h in sealing container, pass through volatility
Chlorine dioxide steam treatment gelatin film, treated, and gelatin film is placed in drying box dry 8h, and then ventilation process makes gelatin for 24 hours
Film surface chlorine dioxide volatilizees completely, is then cleaned 3 times with deionized water, dry.
Embodiment 2
A kind of raising water-soluble processing method of electrostatic spinning gelatin film
1) animal skin gelatin is dissolved in NaCl solution, the magnetic agitation 18h at 60 DEG C obtains the homogeneous bright of 30%w/v
Sol solution；Then gelatin solution is placed in syringe, electrostatic spinning gelatin film is collected by method of electrostatic spinning；Electrostatic spinning mistake
Journey, electrostatic spinning voltage are 18KV, and syringe needle is 17cm away from receiver distance, and the propulsion flow rate for promoting pump is 0.32ml/h；
2) by K2S2O2It adds in deionized water solution, stirs to after being completely dissolved, add the NaClO of equivalent2, stirring
Uniformly obtain 1.2g/L ClO 2 solutions；
By above-mentioned electrostatic spinning gelatin film with ClO 2 solution together be placed in 14h in sealing container, pass through volatility
Chlorine dioxide steam treatment gelatin film, treated, and gelatin film is placed in drying box dry 12h, and then ventilation process 18h makes bright
Film surface chlorine dioxide volatilizees completely, is then cleaned 3 times with deionized water, dry.
Embodiment 3
A kind of raising water-soluble processing method of electrostatic spinning gelatin film
1) animal skin gelatin is dissolved in NaCl solution, magnetic agitation for 24 hours, obtains the homogeneous bright of 28%w/v at 55 DEG C
Sol solution；Then gelatin solution is placed in syringe, electrostatic spinning gelatin film is collected by method of electrostatic spinning；Electrostatic spinning mistake
Journey, electrostatic spinning voltage are 16KV, and syringe needle is 15cm away from receiver distance, and the propulsion flow rate for promoting pump is 0.30ml/h；
2) by K2S2O2It adds in deionized water solution, stirs to after being completely dissolved, add the NaClO of equivalent2, stirring
Uniformly obtain 0.1/L ClO 2 solutions；
By above-mentioned electrostatic spinning gelatin film with ClO 2 solution together be placed in 12h in sealing container, pass through volatility
Chlorine dioxide steam treatment gelatin film, treated, and gelatin film is placed in drying box dry 10h, and then ventilation process makes bright for 24 hours
Film surface chlorine dioxide volatilizees completely, is then cleaned 3 times with deionized water, dry.
Embodiment 4
A kind of raising water-soluble processing method of electrostatic spinning gelatin film
1) 2.8 grams of gelatin are weighed to be dissolved in the NaCl aqueous solutions of 10ml, stirred at 50 DEG C for 24 hours, obtain 28% gelatin it is molten
Liquid.The gelatin solution of gained is placed in 10ml syringes, syringe is put on electrostatic spinning machine, sets spinning parameter, and voltage is adjusted
To 16KV, pump fltting speed is promoted to be set as 0.3ml/h, syringe needle is 15cm away from receiver distance, and about 0.5mm is collected in collector
The gelatin spinning film of thickness；
2) 0.5 gram of K is weighed respectively2S2O2And NaClO2It pours into the beaker for filling 1L deionized water solutions, glass bar stirring
To being completely dissolved, aqueous solution of chlorine dioxide is obtained；Aqueous solution of chlorine dioxide and gelatin spinning film are placed in closed container simultaneously
12h obtains the electrostatic spinning gelatin film after chlorine dioxide steam treatment.
Fig. 1 illustrates the scanning electron microscope (SEM) photograph of the forward and backward electrostatic spinning gelatin film of chlorine dioxide steam treatment in embodiment 4,
It is clear that from figure and successfully prepares 100 nanometers of electrostatic spinning gelatin film, and chlorine dioxide steam treatment caudacoria becomes
Closely.
Fig. 2 illustrates the infared spectrum (FTIR) of the electrostatic spinning gelatin film after chlorine dioxide steam treatment in the present invention,
Gelatin characteristic infrared peak (Figure 1A) is clear that from figure, and gelatin film is in 1102cm after chlorine dioxide treatment‐1(C‐O‐C)
Locate characteristic peak to disappear.
Embodiment 5
The measure of the mechanical performance of electrostatic spinning gelatin film：Gelatin film prepared by embodiment 4, is cut into 5*10mm respectively2
Size, test mechanical performance.
As a result show that the gelatin film mechanical strength after chlorine dioxide treatment increases to 22.42MPa by the 9.30MPa of former film
(Fig. 3), this just illustrates that the gelatin film after chlorine dioxide treatment shows good mechanical performance.
Embodiment 6
The water-soluble measure of electrostatic spinning gelatin film：Clip quality is the former film of 0.05g and the chlorine dioxide of 0.05g respectively
Treated gelatin film, is placed in the PBS solution of PH=7.4, shaken in 37 DEG C of incubator 10min, 20min, 30min,
40min、50min、60min.Then the quality of each time interval film is weighed.By swelling ratioIt carries out molten
Swollen rate calculates, and the water solubility of film is assessed by swelling ratio.
Wherein：Ws is wet film weight, and Wd is dry film weight.
The result shows that the gelatin membrane swelling ratio after chlorine dioxide treatment is finally reached 180% (Fig. 4), and former film is
850%, treated that gelatin film water solubility is greatly improved for this explanation.Show that the film after chlorine dioxide treatment has possessed
Good water solubility has very big value for the practical application of gelatin film.
Embodiment 7
The Cytotoxicity evaluation of the electrostatic spinning gelatin film of chlorine dioxide treatment：Mescenchymal stem cell is used for assessing titanium dioxide
The cytotoxicity of chlorine treated gelatin film.About 105A mescenchymal stem cell is seeded in respectively is placed with 2*2*1mm3At chlorine dioxide
In 24 culture plates of the gelatin film after reason and in 24 orifice plate of blank, add in containing bovine serum albumin(BSA) and dual anti-height sugar culture
Base is incubated 1,3,5,7 day respectively.MTT is added in after each time interval and is incubated four hours, then adds in DMSO concussions
OD values are surveyed at 15min, 570nm respectively.
The results are shown in Figure 5, the OD values that the gelatin film experimental group by chlorine dioxide treatment is surveyed, at 1,3 day slightly below
Control group, caused by the chlorine dioxide of this may be that treated gelatin film remained on surface.And in the 5th, 7 day its OD value with two
The volatilization of chlorine monoxid is higher than blank group, this shows that the gelatin film after chlorine dioxide treatment almost without cytotoxicity, remains original
The bioactivity of film, the gelatin film handled by this method have good bioactivity.
Embodiment 8
The external biological Bioactivity evaluations of the electrostatic spinning gelatin film of chlorine dioxide treatment：About 105A mesenchyma is done carefully
Born of the same parents are seeded in are placed with 2*2*1mm respectively3In 24 culture plates of the gelatin film after chlorine dioxide treatment, addition contains bovine serum albumin
White and dual anti-high glucose medium is incubated 1,3,5,10 day respectively.Then take out the gelatin film of co-cultivation, successively with 70%,
80%th, 90%, 100% ethanol dehydration 2 minutes, dewatered gelatin film fixes 4h with 5% formalin.Scanning electron microscope
(SEM) adherency growing state of the observation cell on gelatin film.
Fig. 6 illustrates adherency upgrowth situation of the cell on chlorine dioxide gelatin film.There is a small amount of cell adherence within first day
In gelatin film surface, third day starts to increase, and with the variation of time, cell was largely adhered to gelatin film surface at the 5th day,
Cell is largely moved to inside gelatin film when reaching the tenth day, forms one layer of cells layer, and entire period inner cell
It is all grown on gelatin film mellow and full in good shape.This shows that the gelatin film of chlorine dioxide treatment has good biocompatibility, is
A kind of good timbering material, has broad application prospects.
Claims (9)
1. a kind of raising water-soluble processing method of electrostatic spinning gelatin film, it is characterised in that：It includes the following steps：
1）The gelatin solution of 25-30% w/v is placed in syringe, electrostatic spinning gelatin film is collected by method of electrostatic spinning；
2）10-14h in sealing container will be placed in together with the electrostatic spinning gelatin film and the ClO 2 solution of 0.8-1.2g/L,
By volatility chlorine dioxide steam treatment gelatin film, by treated, gelatin film is dried, and then ventilation process 18-24 h make bright
Film surface chlorine dioxide volatilizees completely, then washes with water, dry.
2. a kind of raising water-soluble processing method of electrostatic spinning gelatin film according to claim 1, it is characterised in that：
The preparation method of institute's gelatine solution is as follows：Gelatin is dissolved in NaCl solution, 18-24 h are stirred at 50-60 DEG C, are obtained bright
Sol solution.
3. a kind of raising water-soluble processing method of electrostatic spinning gelatin film according to claim 2, it is characterised in that：Institute
Glue is stated clearly as animal bone gelatin or animal skin gelatin.
4. a kind of raising water-soluble processing method of electrostatic spinning gelatin film according to claim 2, it is characterised in that：
The molecular weight of the gelatin is 20-180 KD.
5. a kind of raising water-soluble processing method of electrostatic spinning gelatin film according to claim 1, it is characterised in that：
The electrostatic spinning process, electrostatic spinning voltage are 14-18 KV, and syringe needle is 13-17 cm away from receiver distance, promotes pushing away for pump
It is 0.28-0.32 ml/h into flow rate.
6. a kind of raising water-soluble processing method of electrostatic spinning gelatin film according to claim 1, it is characterised in that：
Collected electrostatic spinning gelatin film thickness is 0.45-0.55 mm, and spinning is 150-200 nanometers a diameter of.
7. a kind of raising water-soluble processing method of electrostatic spinning gelatin film according to claim 1, it is characterised in that：Institute
The preparation method for stating ClO 2 solution is as follows：By K2S2O2It adds in deionized water solution, stirs to after being completely dissolved, then add
Enter NaClO2And be uniformly mixing to obtain ClO 2 solution, be subsequently placed in 4 DEG C of environment seal it is spare.
8. a kind of raising water-soluble processing method of electrostatic spinning gelatin film according to claim 6, it is characterised in that：Institute
State K2S2O2And NaClO2Mass ratio be 1:1.
9. the electrostatic spinning gelatin film obtained according to any processing methods of claim 1-8 answering in tissue engineering bracket material
With.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114188659A (en) * | 2021-12-07 | 2022-03-15 | 浙江工业大学 | Preparation and application of gelatin fiber diaphragm for lithium metal battery |
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