CN108195804A - A kind of fluorescent probe - Google Patents
A kind of fluorescent probe Download PDFInfo
- Publication number
- CN108195804A CN108195804A CN201711313316.6A CN201711313316A CN108195804A CN 108195804 A CN108195804 A CN 108195804A CN 201711313316 A CN201711313316 A CN 201711313316A CN 108195804 A CN108195804 A CN 108195804A
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- CN
- China
- Prior art keywords
- dichroscope
- fluorescent probe
- display screen
- stop sleeve
- touch display
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
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- Health & Medical Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention belongs to photodetector technical fields, and in particular to a kind of fluorescent probe.The detector light path part includes excitation light source, diaphragm, optical filter, dichroscope, convex lens, and the fluorescence being excited finally is reflected on CCD, and light intensity signal is converted to electric signal and visual result is shown in touch display screen through processor processing by CCD；Touch display screen allows user to set light intensity alarm threshold value in touch display screen, this instruction is transported to microprocessor execution；When detection window is detected, testing result is visually displayed in touch display screen sample, once result of detection is more than the alarm threshold value of setting, machine can send out alarm.This system has the characteristics that small, at low cost, high sensitivity, visual result and can set alarm threshold value.In addition to the totally enclosed structure of detecting window can contact ultraviolet light to avoid human eye.
Description
Technical field
The invention belongs to photodetector technical fields, and in particular to a kind of fluorescent probe.
Background technology
Fluorescer also known as fluorescent whitening agent are a kind of organic compounds.It is indigo glimmering that it can absorb ultraviolet light generation
Light, complementary color becomes white light each other with original yellow light, improves the whiteness of product in the sunlight.Fluorescer is widely used in spinning
It knits, multiple fields, the relevant national standard such as papermaking and washing also allow manufacturer to add fluorescer within the limits prescribed.Fluorescer
Although a kind of comparatively safe chemical substance, but if being contacted excessively to fluorescer, such as the clothing of people's daily life contact
Object, food contain excessive fluorescer, can have potential threaten to the health of people.Qualified purple light flashlight can be examined on the market
The fluorescer in clothing is measured, but cannot judge whether its content is exceeded.
Invention content
It is an object of the invention to be directed to problems of the prior art to provide a kind of fluorescent probe, the fluorescence detection
Fluorescer can be effectively detected out in device, and can qualitatively judge its content；The detector is it is possible to prevente effectively from human eye contact is ultraviolet
Light, and alarm threshold value can be set.
The technical scheme is that：
A kind of fluorescent probe, including chassis body, chassis body is cuboid, and chassis body includes four cabinet sides
Panel, cabinet top panel and chassis backplane, cabinet top panel lower section set gradually excitation light source, diaphragm and exciter filter, machine
Detection window is offered on bottom plate, corresponds to and places sample, the diaphragm below the position of the detection window on chassis backplane
It is connected between excitation light source by stop sleeve, is provided with dichroscope below exciter filter, dichroscope is set to two
On Look mirror fixing bracket, the support bracket fastened top of dichroscope, lower section are fixed respectively by stop sleeve, and dichroscope is fixed
The lower section of stent is by stop sleeve setting corresponding with the detection window on chassis backplane, below dichroscope fixing bracket
The first convex lens of setting in stop sleeve, the corresponding setting of stop sleeve above and below dichroscope fixing bracket；It is described
The side of dichroscope connected by stop sleeve with slit, set successively from left to right in the stop sleeve of dichroscope side
Transmitting optical filter and the second convex lens are equipped with, cabinet side panel is just corresponding to the position setting circuit board of slit, fixed on circuit board
Photodetector and microprocessor are set.
Specifically, the slit is near the focus 3mm of the second convex lens.
Specifically, photodetector includes ccd data acquisition module.
Specifically, the cabinet side panel of the setting circuit board is case front panel, it is provided on case front panel tactile
Display screen is touched, touch display screen is connect with circuit board.
Specifically, the dichroscope fixing bracket is screwed in chassis body.
Specifically, the position of the diaphragm and exciter filter can mutually transfer up and down.
Specifically, the microprocessor model is STMS2F103.
The beneficial effects of the invention are as follows：Fluorescent probe the present invention is based on ccd array is with small, at low cost, smart
High advantage is spent, while there is man-machine interface, light intensity alarm threshold value can be set.Ultraviolet source is integrated in cabinet inside, except spy
It is totally-enclosed outside survey window, it can contact ultraviolet light to avoid human eye in this way.
Description of the drawings
Fig. 1 is the assembling configuration schematic diagram of fluorescent probe of the present invention；
Fig. 2 is the principle schematic of fluorescent probe of the present invention.
1 excitation light source, 2 diaphragms, 3 exciter filters, 4 dichroscopes, 5 first convex lenses, 6 samples, 7 transmitting optical filters, 8
Second convex lens, 9 slits, 10CCD data acquisition modules, 11 circuit boards, 12 touch display screens, 13 cabinet top panels, 14 supports
Sleeve, 15 screws, 16 dichroscope fixing brackets, 17 chassis backplanes, 18 detection windows, 19 case front panels, 20 cabinet sides
Plate, 21 chassis bodies, 111 microprocessors.
Specific embodiment
It is as shown in Figure 1 a kind of structure diagram of fluorescent probe, including chassis body 21, chassis body 21 is rectangular
The bodily form, chassis body 21 include four cabinet side panels 20, cabinet top panel 13 and chassis backplane 17, below cabinet top panel according to
Secondary setting excitation light source 1, diaphragm 2 and exciter filter 3, for obtaining thin excitation beam, exciter filter 3 is used for diaphragm 2
The position of veiling glare in filtering exciting light, the diaphragm 2 and exciter filter 3 can mutually transfer up and down.It is opened on chassis backplane 17
Equipped with detection window 18, sample 6,2 He of diaphragm are placed below the position of the detection window 18 on corresponding chassis backplane 17
It is connected between excitation light source 1 by stop sleeve 14,3 lower section of exciter filter is provided with dichroscope 4, and dichroscope 4 is used for
Through exciting light, reflected fluorescent light, can also reflected excitation light, through fluorescence.Dichroscope 4 is set to dichroscope fixing bracket
On 16, the dichroscope fixing bracket 16 is fixed on by screw 15 in chassis body 21.Dichroscope fixing bracket 16
Top, lower section fixed respectively by stop sleeve 14, the stop sleeve 14 on dichroscope 4 and each position is in 45 degree respectively
Angle, the lower section of dichroscope fixing bracket 16 are set by the way that stop sleeve 14 is corresponding with the detection window 18 on chassis backplane 17
It puts.
The first convex lens 5 of setting in the stop sleeve of 16 lower section of dichroscope fixing bracket, the first convex lens 5 are used to assemble
Excitation beam and fluorescent light beam.14 corresponding setting of stop sleeve above and below dichroscope fixing bracket 16；Described
The side of dichroscope 4 is connected by stop sleeve 14 with slit 9, in the stop sleeve 14 of 4 side of dichroscope from left to right
Transmitting 7 and second convex lens 8 of optical filter is disposed with, transmitting optical filter 7 is used to filter the exciting light in fluorescence, and described is narrow
Seam 9 is near the focus 3mm of the second convex lens 8.The position setting circuit board 11 of the just corresponding slit 9 of cabinet side panel 20, circuit
Photodetector and microprocessor 111 are fixedly installed on plate 11, the microprocessor model is STMS2F103.Photodetection
Device includes ccd data acquisition module 10.The cabinet side panel 20 of described setting circuit board 11 is case front panel 19, before cabinet
Touch display screen 12 is provided on panel, touch display screen 12 is connect with circuit board 11.
The ccd data acquisition module 10 for the fluorescence optical signals received to be converted to electric signal, is output to micro- place
Manage device 111.Microprocessor 111 is used to handle the electric signal that the transmission of ccd data acquisition module 10 comes, and is converted into and the sample
The parameter of the corresponding fluorescent intensity of product is shown in touch display screen 12, while receives the instruction that touch display screen 12 transmits；It touches
Display screen 12 is touched to be used for showing testing result and input alarm threshold value.
The principle schematic of fluorescent probe of the present invention is illustrated in figure 2, the ultraviolet light beam that excitation light source 1 is emitted is through diaphragm
2, into a tiny light beam, the miscellaneous peak in ultraviolet light in addition to excitation peak, the exciting light that filtration treatment is crossed are filtered off by exciter filter 3
It is focused at sample 6 through dichroscope 4, the first convex lens 5, dichroscope 4 transmits exciting light, reflected fluorescent light；Dichroscope 4
Light path to sample 6 is exciting light and fluorescence common optical pathways；The ultraviolet excitation that the fluorescer having in sample 6 is focused, spoke
Penetrate fluorescence.Fluorescence is reflected after penetrating the first convex lens 5 by dichroscope 4, and reflected light is focused at ccd data through the second convex lens 8
On acquisition module 10, slit 9 is added in wherein between 10 and second convex lens 8 of ccd data acquisition module, fluorescence is made to be irradiated into threadiness
On CCD linear array, the second convex lens 8 plays the role of assembling fluorescence, and transmitting optical filter 7 filters out the exciting light in fluorescence,
It avoids interfering.
10 optical signals received of ccd data acquisition module are transformed into electric signal transmission to microprocessor 111, microprocessor
Result is shown in touch display screen 12 after the processing of device 111.Alarm threshold value, while alarm threshold value are inputted from touch display screen 12
This instruction is delivered to microprocessor 111, and when light intensity reaches the threshold value of setting, fluorescent probe can alarm, and reach detection
The purpose of fluorescence.
Finally it should be noted that:The above embodiments are merely illustrative of the technical scheme of the present invention and are not intended to be limiting thereof；To the greatest extent
The present invention is described in detail with reference to preferred embodiments for pipe, those of ordinary skills in the art should understand that：Still
It can modify to the specific embodiment of the present invention or equivalent replacement is carried out to some technical characteristics；Without departing from this hair
The spirit of bright technical solution should all cover in the claimed technical solution range of the present invention.
Claims (7)
1. a kind of fluorescent probe, including chassis body (21), chassis body (21) is cuboid, and chassis body (21) includes
Four cabinet side panels (20), cabinet top panel (13) and chassis backplane (17), cabinet top panel lower section set gradually exciting light
Source (1), diaphragm (2) and exciter filter (3) offer detection window (18), corresponding chassis backplane on chassis backplane (17)
(17) sample (6) is placed below the position of the detection window (18) on, which is characterized in that
It is connected by stop sleeve (14) between the diaphragm (2) and excitation light source (1), is set below exciter filter (3)
There is dichroscope (4), dichroscope (4) is set on dichroscope fixing bracket (16), dichroscope fixing bracket (16)
Top, lower section are fixed by stop sleeve (14) respectively, and the lower section of dichroscope fixing bracket (16) passes through stop sleeve (14)
Setting corresponding with the detection window (18) on chassis backplane (17), in the stop sleeve below dichroscope fixing bracket (16)
First convex lens (5) is set, stop sleeve (14) corresponding setting above and below dichroscope fixing bracket (16)；
The side of the dichroscope (4) is connected by stop sleeve (14) with slit (9), the branch of dichroscope (4) side
Transmitting optical filter (7) and the second convex lens (8), cabinet side panel (20) face are disposed in support set cylinder (14) from left to right
It answers the position of slit (9) that circuit board (11) is set, photodetector and microprocessor (111) is fixedly installed on circuit board (11).
2. fluorescent probe according to claim 1, which is characterized in that the slit (9) is in the second convex lens (8)
Near focus 3mm.
3. fluorescent probe according to claim 1, which is characterized in that photodetector includes ccd data acquisition module
(10)。
4. fluorescent probe according to claim 1, which is characterized in that the cabinet side of the setting circuit board (11)
Plate is case front panel (19), and touch display screen (12), touch display screen (12) and circuit are provided on case front panel (19)
Plate (11) connects.
5. fluorescent probe according to claim 1, which is characterized in that the dichroscope fixing bracket (16) passes through
Screw (15) is fixed in chassis body (21).
6. fluorescent probe according to claim 1, which is characterized in that the position of the diaphragm (2) and exciter filter (3)
Put mutually to transfer up and down.
7. fluorescent probe according to claim 1, which is characterized in that described microprocessor (111) model is
STMS2F103。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711313316.6A CN108195804A (en) | 2017-12-12 | 2017-12-12 | A kind of fluorescent probe |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711313316.6A CN108195804A (en) | 2017-12-12 | 2017-12-12 | A kind of fluorescent probe |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108195804A true CN108195804A (en) | 2018-06-22 |
Family
ID=62574111
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711313316.6A Pending CN108195804A (en) | 2017-12-12 | 2017-12-12 | A kind of fluorescent probe |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108195804A (en) |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5583342A (en) * | 1993-06-03 | 1996-12-10 | Hamamatsu Photonics K.K. | Laser scanning optical system and laser scanning optical apparatus |
| US20040246573A1 (en) * | 2003-05-21 | 2004-12-09 | Olympus Corporation | Total reflection fluorescent microscope |
| US20070132994A1 (en) * | 2005-12-02 | 2007-06-14 | Nanophoton Corp. | Optical microscope and spectrum measuring method |
| US20110127406A1 (en) * | 2007-09-03 | 2011-06-02 | Nikon Corporation | Auto focus apparatus and microscope |
| CN202563160U (en) * | 2012-04-11 | 2012-11-28 | 上海理工大学 | Imaging system which realizes multipath frequency division multiplexing fluorescence confocal microscopy by coaxial optical paths |
| CN102841083A (en) * | 2012-06-11 | 2012-12-26 | 北京大学 | Method and system of laser scanning phase-microscope imaging |
| US20140291547A1 (en) * | 2013-03-27 | 2014-10-02 | Logos Biosystems, Inc. | Fluorescence imaging device |
| CN104990902A (en) * | 2015-06-24 | 2015-10-21 | 石家庄经济学院 | Plant chlorophyll fluorescence detection device based on LED |
| CN106290267A (en) * | 2015-05-18 | 2017-01-04 | 北京怡成生物电子技术股份有限公司 | Fluorescence detection device |
| CN207850924U (en) * | 2017-12-12 | 2018-09-11 | 濮阳光电产业技术研究院 | A kind of fluorescent probe |
-
2017
- 2017-12-12 CN CN201711313316.6A patent/CN108195804A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5583342A (en) * | 1993-06-03 | 1996-12-10 | Hamamatsu Photonics K.K. | Laser scanning optical system and laser scanning optical apparatus |
| US20040246573A1 (en) * | 2003-05-21 | 2004-12-09 | Olympus Corporation | Total reflection fluorescent microscope |
| US20070132994A1 (en) * | 2005-12-02 | 2007-06-14 | Nanophoton Corp. | Optical microscope and spectrum measuring method |
| US20110127406A1 (en) * | 2007-09-03 | 2011-06-02 | Nikon Corporation | Auto focus apparatus and microscope |
| CN202563160U (en) * | 2012-04-11 | 2012-11-28 | 上海理工大学 | Imaging system which realizes multipath frequency division multiplexing fluorescence confocal microscopy by coaxial optical paths |
| CN102841083A (en) * | 2012-06-11 | 2012-12-26 | 北京大学 | Method and system of laser scanning phase-microscope imaging |
| US20140291547A1 (en) * | 2013-03-27 | 2014-10-02 | Logos Biosystems, Inc. | Fluorescence imaging device |
| CN106290267A (en) * | 2015-05-18 | 2017-01-04 | 北京怡成生物电子技术股份有限公司 | Fluorescence detection device |
| CN104990902A (en) * | 2015-06-24 | 2015-10-21 | 石家庄经济学院 | Plant chlorophyll fluorescence detection device based on LED |
| CN207850924U (en) * | 2017-12-12 | 2018-09-11 | 濮阳光电产业技术研究院 | A kind of fluorescent probe |
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