CN108185429A - A kind of method using extra-high tension unit extraction fresh ginseng and its purposes in healthy food - Google Patents
A kind of method using extra-high tension unit extraction fresh ginseng and its purposes in healthy food Download PDFInfo
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- CN108185429A CN108185429A CN201710359877.3A CN201710359877A CN108185429A CN 108185429 A CN108185429 A CN 108185429A CN 201710359877 A CN201710359877 A CN 201710359877A CN 108185429 A CN108185429 A CN 108185429A
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- 238000000034 method Methods 0.000 title claims abstract description 15
- 238000000605 extraction Methods 0.000 title claims abstract description 13
- 235000001497 healthy food Nutrition 0.000 title description 2
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/36—Caryophyllaceae (Pink family), e.g. babysbreath or soapwort
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Alternative & Traditional Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Medical Informatics (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention provides a kind of methods using superhigh pressure technique extraction fresh ginseng, in brief, select life in high-quality 45 years that there is the fresh ginseng of complete exocuticle, add 0.5 1 times of water, people participates in water merging polybag and then seals, polybag is put into superelevation ballasting, 400 megapascal pressure, extraction 5 minutes, takes extracting solution to filter, obtains fresh ginseng extracting solution.Specimen material is not required to crush in extraction process, keeps whole branch fresh ginseng prototype, and exocuticle is intact, so as to utilize the property of this natural filter membrane of ginseng crust, only relatively small Middle Molecular Substance prevents passing through for part macromolecular compound, ensure that the clear and bright of fresh ginseng stoste by crust.Fresh ginseng oral liquid or drink can be prepared using fresh ginseng extracting solution, has and is substantially reduced diabetic mice fasting blood-glucose, hence it is evident that improves diabetes B Mouse oral sugar tolerance, while does not increase blood insulin levels.It can be as drug, food or the health products for preparing prevention diabetes B.
Description
Technical field
The present invention relates to ultra high pressure extraction technologies to prepare fresh ginseng and fresh ginseng method for preparing beverage.
Background technology
Ginseng is China's tradition rare medicinal herbs, has and reinforces vital energy, and veins takes off admittedly reinforces the spleen to benefit the lung, and promotes the production of body fluid to quench thirst, benefit of calming the nerves
The effect of intelligence.By domestic and foreign scholars, constantly research and discovery finds that the chief active in ginseng is that ginsenoside, ginseng are more
Sugar, in addition with chemical compositions such as protein, polypeptide, amino acid, trace elements.These substances play it for ginseng and improve body
The pharmacological activity such as immunity, enhancing memory, antianxiety, anticancer, hypoglycemic provide the foundation [1,2].Ginseng is from ancient times just useful
In the record for the treatment of " quenching one's thirst ", although quenching one's thirst not exclusively is equal to diabetes, modern pharmacology experiment has been proved in ginseng
The substances such as ginsenoside, panaxan, polypeptide all have the function of to anti-diabetic and its complication [3-5].
Insulin resistance（Insulin Resistance, IR）It is that academia fell over each other one of hot spot inquired into recent years,
With the continuous heating of research, the research field in relation to insulin resistance gradually expands to angiocardiology from endocrine subject
Among multiple cross disciplines such as section, nephrosis subject and neuropathy subject.It is reported that relatively have for treatment insulin resistance at present
The drug of effect is alkane diones.Such as Pioglitazone, it can be by improving the pancreas of the muscle of diabetes B patient, fat, liver
Insulin resistance, so as to reach the function [6] of protection beta Cell of islet.But these Western medicine all have the treatment of insulin resistance
Certain side effect [7].Therefore, one kind is found naturally, the drug of low side effect has great importance.Sheth etc. [8] is pointed out
Abscisic acid (ABA) plays the role of insulin resistance rat to adjust improvement.Recent research indicate that[9]Oral 0.5-1 μ g/kg
ABA show and reduce rat and blood sugar for human body and insulinemia.Therefore, the hypoglycemic effect of internal low dosage ABA does not depend on
Increase in insulin releasing, low dosage ABA intakes, which can be used as, to be helped to improve diabetic's insulin resistance or improve sugar
Tolerance.People β islet cells and rat myoblasts are stimulated to increase the intake of insulin releasing and sugar with ABA respectively in vitro,
Minimum ABA actions concentration is 1.0 and 0.1nM respectively, this is presented as myocyte has higher sensibility to ABA.
Abscisic acid is a Plant Hormone, it is prevalent in mammal and plant, various humans and animals tissues
ABA is discharged under physiology or incentive condition with cell, and immunocyte, cardiovascular organization and cell, pancreatic cell and stem cell
With important regulating and controlling effect, it is considered to be the extensive general signal factor of biological world can treat many diseases, for example,
It is anti-inflammatory, cancer, diabetes etc..Content is little in most plants, and there are abundant abscisic acids in this patent announcement fresh ginseng.It takes off
Show that the fresh ginseng extract chief active factor that the art of this patent obtains is abscisic acid, having reduces diabetes B blood glucose, improves
Sugar tolerance, while reduce insulin level in blood.A kind of method using extra-high tension unit extraction effective components in plants
（ZL201510231951.4）Patent is pointed out, powdered plant raw material are fitted into container, adds in water or the leaching of 5%-60% alcohol
Bubble, solid-liquid ratio 1:1-1:3, it is sufficiently stirred, impregnates 4-8 hours, make the abundant water swelling of plant cell, use for reference this patent basis
On, author does not shred fresh ginseng holding archetype state, keeps whole Zhi Rencan exocuticles intact, and using ginseng crust, this is natural
The property of film makes many macromolecular substances, such as protein, polysaccharide etc. are less passes through, this property ensure that extracting solution is clear and bright
The making of oral beverage is more conducive to, so as to save the processes such as a large amount of heating, concentration, filtering, precipitation, reaches energy saving and subtracts
Of low pollution belongs to environmentally friendly processing technology；Solid-liquid ratio is selected to be less than 1, can be increased to greatest extent active in extracting solution
The concentration of factor abscisic acid, and since ultra high pressure extraction process is carried out in room temperature or under may remain in lower temperature,
So as to keep the peculiar various active materials of fresh ginseng institute to greatest extent.
Invention content
The invention discloses a kind of preparation methods of fresh ginseng extract.
Method is as follows：A kind of preparation method of fresh ginseng extract, method are as follows：It is fresh to select high-quality fresh 4-5 lifes
Ginseng, every weight is in 20-40g, it is desirable that disease-free scar, without rust, pollution-free, non agricultural chemical residuum keeps whole Zhi Rencan exocuticles
It is intact, it cleans, adds 0.5-1 times of water, people is participated in water merging polybag, polybag is put into superelevation ballasting by sealing, is pressurizeed
400 megapascal keep pressure time 5 minutes, take out, and discharge solution in bag, and entire extraction process is in room temperature or lower
At a temperature of carry out, add 0.5-1 times of water, repeat the above process, merge No. 2 extracting solutions, filtering, obtain fresh ginseng extracting solution.
The invention discloses abscisic acid is mainly contained with fresh ginseng extract, also ginsenoside, free amino acid etc. is living
Property substance.
Contain abscisic acid in fresh ginseng, water extraction fresh ginseng per kilogram reaches as high as 15mg abscisic acids.
It is resistance in reduction blood glucose, improvement glucose by primary activity ingredient of abscisic acid the invention discloses fresh ginseng extract
Application in terms of amount.The fresh ginseng extract contains abscisic acid, and having prevents insulin resistance, antidiabetic effect, Ke Yiying
For the exploitation of diabetes mellitus prevention and treatment drug, the exploitation of the food and health products of related field can also be applied to.
Can be hypoglycemic while do not increase blood insulin levels the invention discloses the fresh ginseng extract containing abscisic acid, it can drop
The blood glucose of low diabetic and raising Glucose tolerance test, and high blood insulin disease will not be caused, prevention glycosuria can be become
The natural product of disease and auxiliary treatment diabetes.
One or more of functionality with health effect common at present can also be added in pharmaceutical composition of the present invention
Food, so as to play the role of the food compositions of maintenance or the enhancing present invention.
Description of the drawings
Attached drawing 1：The HPLC-MS spectrograms of abscisic acid reference substance
Attached drawing 2：Fresh ginseng water extract HPLC-MS spectrograms
Attached drawing 3：The high-efficient liquid phase chromatogram of abscisic acid reference substance
Attached drawing 4：The high-efficient liquid phase chromatogram of fresh ginseng water extract abscisic acid
Attached drawing 5：Influence of the ginseng extract to diabetes B model mice Glucose tolerance test (OGTT)
Note：Blank control group（A）, model group（B）, melbine group（C）, the basic, normal, high dosage group of ginseng extract（D、E、F）
With blank group, #P＜ 0.05, ##P＜ 0.01；With model group, *P＜ 0.05, * *P1 is comes off in ＜ 0.01, attached drawing 3 and attached drawing 4
Acid.
Bibliography
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from Panax ginseng on type 2 diabetic rats induced by high-fat diet and
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[5] Ma Ping, Hu Rong, Wang little Ying wait progress [J] Hubei University of Chinese Medicine of Chinese medicines ginseng treatment diabetes to learn
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[9]Magnone M, Ameri P, Salis A, et al. Microgram amounts of abscisic acid
in fruit extracts improve glucose tolerance and reduce insulinemia in rats
and in humans[J]. Faseb Journal Official Publication of the Federation of
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Specific embodiment
Embodiment 1
The preparation of fresh ginseng extract：Select high-quality fresh 5 years fresh ginsengs, 100g, it is desirable that disease-free scar, without rust, it is pollution-free,
Non agricultural chemical residuum keeps fresh ginseng exocuticle complete, cleans, add water 90ml, and people is participated in water merging polybag, is sealed, will
Polybag is put into superelevation ballasting, and 400 megapascal of pressurizeing keep pressure time 5 minutes, and release is taken out, and discharges solution in bag,
90ml water is added, people is participated in water merging polybag, polybag is put into superelevation ballasting by sealing, 400 megapascal of pressurizeing,
Keep pressure time 5 minutes, release is taken out, and discharges solution in bag, is then taken out ginseng and is impregnated 5h with 90 ml water again, merges 3
Secondary extracting solution, filtering, obtains filtrate about 260ml.The fresh ginsengs of 100g propose 1.5mg abscisic acids, 15 μ g/g, by proposition liquid after testing
Product calculates：Contain abscisic acid 1.5mg, 5.8 μ g/ml in 260ml solution；The fresh ginsengs of 100g propose general ginsenoside 80mg after testing,
0.8mg/g is calculated by proposing that liquid accumulates：Contain general ginsenoside 80mg, 0.3mg/ml in 260ml solution；100g is fresh after testing
Ginseng proposes free amino acid 2002 mg, 20.02mg/g, is calculated by proposing that liquid accumulates：Contain free amino acid in 260ml solution
2002mg,7.7mg/ml。
Embodiment 2
The preparation of fresh ginseng extract：Select high-quality fresh 5 years fresh ginsengs, 100g, it is desirable that disease-free scar, without rust, it is pollution-free,
Non agricultural chemical residuum is cleaned, and is especially careful not to destroy ginseng crust, is added water 70ml, and people is participated in water merging polybag, close
Envelope, polybag is put into superelevation ballasting, and 400 megapascal of pressurizeing keep pressure time 5 minutes, and release is taken out, and is discharged in bag
Solution adds 70ml water, people is participated in water merging polybag, polybag is put into superelevation ballasting by sealing, pressurization 400
Megapascal, keeps pressure time 5 minutes, and release is taken out, and discharges solution in bag, then takes out ginseng and impregnates 5h with 70 ml water again,
Merge No. 3 extracting solutions, filter, filtering obtains filtrate 200ml.The fresh ginseng proposition 1.35mg abscisic acids of 100g after testing, 13.5 μ g/g,
It is calculated by proposing that liquid accumulates：Contain abscisic acid 1.35mg, 6.75 μ g/ml in 200ml solution；The fresh ginsengs of 100g propose ginseng after testing
Total saposins 60mg, 0.6mg/g are calculated by proposing that liquid accumulates：Contain general ginsenoside 60mg, 0.3mg/ml in 200ml solution；Through
It detects the fresh ginsengs of 100g and proposes free amino acid 1820mg, 18.2mg/g, calculated by proposing that liquid accumulates：In 200ml solution simultaneously also
Contain free amino acid 1820mg, 9.1mg/ml.
Embodiment 3
The preparation of fresh ginseng extract：Select high-quality fresh 5 years fresh ginsengs, 100g, it is desirable that disease-free scar, without rust, it is pollution-free,
Non agricultural chemical residuum is cleaned, and is especially careful not to destroy ginseng crust, is added water 90ml, and people is participated in water merging polybag, close
Envelope, polybag is put into superelevation ballasting, and 400 megapascal of pressurizeing keep pressure time 5 minutes, and solution in bag is taken out in release,
Filtering, obtains filtrate about 90ml, water is added to make into 100ml.The fresh ginsengs of 100g propose 0.625mg abscisic acids, 6.25 μ g/g, by fixed after testing
Hold liquid product to calculate：Containing abscisic acid 0.625mg, 6.25 μ g/ml in 100ml solution, the fresh ginseng proposition ginsengs of 100g are total after testing
Saponin(e 41mg, 0.41mg/g are accumulated by constant volume liquid and calculated：Contain general ginsenoside 41mg, 0.41mg/ml in 100ml solution；Through
It detects the fresh ginsengs of 100g and proposes free amino acid 1200mg, 15.00mg/g, accumulate and calculate by constant volume liquid：Contain in 100ml solution
Free amino acid 1200mg, 12mg/ml；Arginine glucoside after testing（AF）Contain 0.3mg per ml solution.
Embodiment 4
The preparation of fresh ginseng extract：Select high-quality fresh 5 years fresh ginsengs, 100g, it is desirable that disease-free scar, without rust, it is pollution-free,
Non agricultural chemical residuum is cleaned, and is especially careful not to destroy ginseng crust, is added water 60ml, and people is participated in water merging polybag, close
Envelope, polybag is put into superelevation ballasting, and 400 megapascal of pressurizeing keep pressure time 5 minutes, and solution in bag is taken out in release,
Filtering, obtains filtrate 60ml.The fresh ginsengs of 100g propose 0.525mg abscisic acids after testing, and 5.25 μ g/g are calculated by proposing that liquid accumulates：
Contain abscisic acid 0.525mg, 8.41 μ g/ml in 60ml solution；The fresh ginsengs of 100g propose general ginsenoside 30mg, 0.3mg/ after testing
G is calculated by proposing that liquid accumulates：Contain general ginsenoside 30mg, 0.5mg/ml in 60ml solution；The fresh ginsengs of 100g propose trip after testing
Isolated amino acid 900mg, 9.0mg/g；It is calculated by proposing that liquid accumulates：Contain free amino acid 900mg, 15.0mg/ in 60ml solution
ml。
5 fresh ginseng plant beverage of embodiment
1 gained fresh ginseng extracting solution 200ml of Example, adding in 800ml pure water makes into 1000ml, and it is fragrant to add appropriate plant
Smart and appropriate preservative, filtered, embedding, sterilizing, every 50ml, every contains 58 μ g abscisic acids, stores 2 under room temperature
Year, it finds no precipitation and generates, drink remains as clear room temperature liquid.
6 fresh ginseng amino-acid nutrition liquid of embodiment
3 gained fresh ginseng extracting solution 100ml of Example, adding in 400ml pure water makes into 500ml, adds 0.2g STEVIA REBAUDIANAs
Glycosides, appropriate amount of essence per in 100ml nutrient solutions, contain amino acid 300mg, ginsenoside 20mg, 125 μ g of abscisic acid, arginine list
Glucosides（AF）, 6mg.Filtering, embedding sterilize, capping.Every 10ml is stored 2 years under room temperature, is found no precipitation and is generated,
Drink remains as clear room temperature liquid.
7 HPLC-MS of embodiment determines abscisic acid in sample
The come off preparation standard solution precision of acid solution of standard weighs abscisic acid standard items 10.00mg, is dissolved with 80% methanol
Constant volume is in 100ml volumetric flasks, 0.22 μm of filtering with microporous membrane, you can the abscisic acid pair that mass concentration is 0.10mg/mL is made
According to product solution.It is identical that the fresh ginseng extract 5ml that distinct methods obtain in sample solution preparation Example is put into specification
In 250mL conical flasks, 80% methanol of 100mL is added in, is extracted in 4 DEG C of refrigerators, every time four hours, extraction three times, merges three times
Extracting solution after extracting solution is carried out vacuum rotary steam, is adjusted its PH=2.8 ~ 3.0, is extracted with ethyl acetate, and extraction three times, merges
Extracting solution three times carries out vacuum rotary steam again, with 80% methanol constant volume 25mL after being evaporated, with 0.22 μm of filtering with microporous membrane to get
Test solution.
Mass Spectrometry Conditions ESI ion sources, anion mode determination；Guard column：Phenomenex (4.0 × 3.0mm, 5 μm)；
Chromatographic column：ODS-MG C18 liquid-phase chromatographic columns (4.6 × 150mm, 5 μm)；Mobile phase：Methanol-water (55：45), flow velocity：0.8
mL/min；Column temperature：45°；Ion source temperature：500℃；Atomization gas：50 psi；Assist gas：50 psi；Gas curtain gas：30 psi；Point
Stream ratio：1（Into mass spectrum）：3（Shunting）.；Sample size：5μL.
Determine that abscisic acid molecular weight is consistent with standard items for 263.1 in sample by HPLC-MS（Attached drawing 1 and attached drawing 2）.
ABA content assay method in 8 fresh ginseng extract of embodiment
The preparation standard solution precision of 1 solution weighs abscisic acid standard 10.00mg, with 80% methanol dissolving constant volume in
In 100ml volumetric flasks, 0.22 μm of filtering with microporous membrane, you can it is molten that the abscisic acid reference substance that mass concentration is 0.10mg/mL is made
Liquid.
It is identical that the fresh ginseng extract 1g that distinct methods obtain in test solution preparation Example is each placed in specification
250mL conical flasks in, add in 80% methanol of 100mL, extracted in 4 DEG C of refrigerators, every time four hours, extraction three times, merges three
Secondary extracting solution after extracting solution is carried out vacuum rotary steam, is adjusted its PH=2.8 ~ 3.0, is extracted with ethyl acetate, and extraction three times, is closed
And extracting solution three times, vacuum rotary steam is carried out again, with 80% methanol constant volume 25mL after being evaporated, with 0.22 μm of filtering with microporous membrane, i.e.,
Obtain test solution.
Chromatographic condition chromatographic column：Wakopak C18 (250mm × 4.6mm, 5 μm）；Column temperature：45℃；Mobile phase：First
‰ phosphoric acid water of alcohol -2（V（Methanol）：V（Water）=55：45）；Flow velocity：0.8mL/min；Examine wavelength：260nm；Sample size：20μL.
Chromatogram is shown in attached drawing 3 and attached drawing 4.
Influence of 9 ginseng extract of embodiment to blood glucose in diabetic mice, sugar tolerance, serum insulin
Drug prepares positive drug solns：250mg melbine is taken to be dissolved in 1 ‰ carboxymethylcellulose sodium solutions, is configured to
25mg·mL-1Solution.
In 3 Ginseng extract 1ml of sample solution difference Example, 2 ml, 4 ml difference constant volumes to 10ml volumetric flasks,
Up to 0.625,1.25,2.50 μ gmL-1Ginseng extract（Contain 0.625,1.25,2.50 μ g abscisic acids respectively per ml）.
Animal model and grouping
ICR male mices 120 after adaptability is raised 7 days, randomly select 10 as blank control group, feed chow diet, other
It 110, feeds high lipid food 30 days, was deprived of food but not water in the 30th day night, randomly selects 30 eye sockets within the 31st day and take blood, centrifuging and taking
Serum surveys TC, TG, higher than normal value, such as table 1.100mg/kgSTZ citrate buffer solutions are injected intraperitoneally（0.1M PH=4.4）, after
Continuous to feed high lipid food 10 days, the 10th day night was deprived of food but not water, and takes a blood sample in the 11st day tail portion and measures blood glucose value, and survey fasting blood-glucose >=
11.1mmol/L is modeling success, is selected to 80 mouse in mould, is randomly divided into 8 groups, respectively model group, melbine group
（250 mgkg of melbine-1）, the high, medium and low dosage group of ginseng extract（25.00、12.50、6.25μg·kg-1It comes off
Acid）, every group 10, blank control group and the daily gavage physiological saline of model group, other administration groups distinguish gavage relative medicine,
0.1mL/10g dosage is administered, and is administered 40 days altogether, records mouse bioavailability and weight daily.
Statistical method
Using SPSS17.0 statistics softwares, experimental data uses variance analysis with means standard deviation comparison among groups, using LSD
Method is compared two-by-two.P ＜ 0.05 represent that difference has statistical significance.
As a result
Influence to diabetes B model mice fasting blood-glucose and serum insulin
Mouse fasting blood sugar is significantly raised after modeling success, with the significant difference of blank control group（P ＜ 0.01）, administration controls
After treatment, experimental mice fasting blood sugar is on a declining curve.After continued administration four weeks, compared with model group, melbine group,
Ginseng extract high dose group（25.00μg·kg-1Abscisic acid）With ginseng extract middle dose group（12.50μg·kg-1）It comes off
Acid, fasting blood sugar significantly reduce（P ＜ 0.01）, other administration group mouse fasting blood sugars also decrease, but without apparent poor
It is different, it is not statistically significant.It the results are shown in Table 1.Measurement result of the table 1 for INS in mice serum, the INS and model group of each administration group
Mouse is compared, and has pole significant difference（P<0.01）, it is shown in Table 1.
Influence of 1 ginseng extracts of table to blood glucose in diabetic mice, serum insulin
| Group | 2 weeks | 4 weeks | INS(mIU/L) |
| Blank group | 4.57±0.61 | 5.4±0.86 | 16.79±1.01 |
| Model group | 12.44±8.23## | 16.73±3.88## | 25.23±4.17## |
| Melbine group | 9.41±5.08 | 11.87±7.41 | 19.14±1.52** |
| Ginseng extract（It is high） | 8.77±5.08 | 10.64±8.13* | 20.81±3.26** |
| Ginseng extract（In） | 7.53±4.27* | 11.33±5.63* | 20.49±2.48** |
| Ginseng extract（It is low） | 8.52±2.05 | 10.53±6.62* | 18.83±3.21** |
Compared with blank group## P<0.01；Compared with model group* P<0.05,** P<0.01
Influence to diabetes B mouse glucose tolerance amount
As shown in Figure 5, ginseng extract high dose group is significantly lower than model group with middle dose group Area under the curve of blood glucose AUC,
And with pole significant difference（P＜ 0.05）.
Claims (5)
1. a kind of preparation method of fresh ginseng extract, method are as follows：It selects high-quality fresh 4-5 and gives birth to fresh ginseng, weight exists
20-40g, it is desirable that disease-free scar, without rust, pollution-free, non agricultural chemical residuum keeps whole Zhi Rencan exocuticles intact, cleans, add water
0.5-1 times, people is participated in water merging polybag, polybag is put into superelevation ballasting by sealing, and 400 megapascal of pressurizeing are kept
Pressure time 5 minutes takes out, and discharges solution in bag, and entire extraction process is carried out under room temperature or lower temperature, then
0.5-1 times of water is added in, is repeated the above process, merges No. 2 extracting solutions, filtering obtains fresh ginseng extracting solution.
2. preparation method according to claim 1, which is characterized in that fresh ginseng can also be fresh American Ginseng, fresh Radix Notoginseng, it is fresh too
The rhizome plants such as son ginseng.
3. fresh ginseng according to claim 2, fresh American Ginseng, it is characterised in that be the intact fresh ginseng of the whole branch exocuticles of 1-6.
4. fresh ginseng extracting solution according to claim 1, it is characterised in that contain abscisic acid, free amine group in extracting solution
The small-molecule substances such as acid, polypeptide, arginine derivative, ginsenoside, vitamin.
5. fresh ginseng extracting solution according to claim 4, it is characterised in that can be applied to food and the health care of related field
The exploitation of product.
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Application publication date: 20180622 |