CN108185176A - With the feedstuff for live pigs for improving immune performance - Google Patents

With the feedstuff for live pigs for improving immune performance Download PDF

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CN108185176A
CN108185176A CN201711237649.5A CN201711237649A CN108185176A CN 108185176 A CN108185176 A CN 108185176A CN 201711237649 A CN201711237649 A CN 201711237649A CN 108185176 A CN108185176 A CN 108185176A
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parts
meretrix
feedstuff
live pigs
fish oil
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孙琦
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Lanxi Unitary Feed Technology Service Co Ltd
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Lanxi Unitary Feed Technology Service Co Ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A23K10/22—Animal feeding-stuffs from material of animal origin from fish
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/105—Aliphatic or alicyclic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/111—Aromatic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/158—Fatty acids; Fats; Products containing oils or fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/174—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/179—Colouring agents, e.g. pigmenting or dyeing agents
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/22—Compounds of alkali metals
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/30—Oligoelements
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

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Abstract

The invention discloses with the feedstuff for live pigs for improving immune performance, the composition and its parts by weight of feedstuff for live pigs are：100 120 parts of fermented bean dregs, 35 parts of Meretrix meretrix Glycopeptide, 23 parts of fish oil nano-emulsion, 12 parts of Chinese medicine preparation, 0.1 0.2 parts of Yolk antibody, 0.2 0.3 parts of sodium butyrate, 1.2 1.5 parts of carrotene, 34 parts of chitosan, 10 20 parts of sodium chloride, 0.5 0.6 parts of multidimensional, 0.6 0.8 parts of more ore deposits, the preparation method of feedstuff for live pigs includes：The preparation of Meretrix meretrix Glycopeptide, prepares fish oil nano-emulsion, the preparation of Chinese medicine preparation, compounding at bean pulp fermentation.It has the beneficial effect that：Feed can be effectively improved the immunocompetence and anti-stress ability of live pig, reduce the harmful intestinal tract bacterial content brought by feed, feed also containing nutriments such as abundant protein, amino acid, microorganism, minerals, can accelerate live pig growth, reduce feeding cost.

Description

With the feedstuff for live pigs for improving immune performance
Technical field
The present invention relates to pannage field, more particularly, to the feedstuff for live pigs for improving immune performance.
Technical background
Pig also known as " coal ", " black flour youth " and " black grandfather ".《The unanimous load of the court and the commonalty》It says, the Tang Dynasty flood people from state pig raising is got rich, and pig is referred to as " coal ".The Tang Dynasty《Cloud celestial being miscellanies》Draw《Peaceful old bun》：" black flour youth calls pig." on the soil of China, early in matriarchy People's commune's period has just started to raise the domestic animals such as pig, dog.The pottery pig that Zhejiang Yuyao He Mudu Neolithic cultures ruins are unearthed, shape It is quite similar with domestic pig body, illustrate had blank to the domestication of pig at that time.Mammalia Artiodactyla Suidae pig category omnivorousness is meat Domestic animal.Domestic pig is tamed by wild boar.Have the characteristics that reproductive capacity is strong, growth is fast, efficiency of feed utilization is high, feeding habits are wide.Pork is thin It is tender delicious, it is full of nutrition, it is one of main meat product of the mankind.Divided by fresh meat is edible outer, is further adapted for being processed into ham, salt down The products such as meat, sausage and dried meat floss.Pigskin, bristles and hog intestine can make the raw material of industry.Pig blood and pig bone can be respectively prepared blood meal and Bone meal is used as feed.The internal organ and body of gland of pig can obtain through refining a variety of Medicines.
The prior art discloses a kind of raising pig and exempts from as authorized the Chinese invention patent that public platform is 102894213 B of CN The mixed feed of epidemic disease power, the inventive method by watermelon peel, carrot dry, add in corn and soybean, dried fish, wheat bran, chitosan oligosaccharide, Sodium chloride, glutamic acid smash it through 40 mesh and sieve to obtain the final product, and processing technology is simple, easy to use, and required nutrition is eaten meeting live pig Outside, be also conducive to enhance the immunity of live pig rich in chitosan oligosaccharide.But in the inventive method soybean, wheat bran it is non-modified or Fermentation is to be fed to live pig, and the anti-nutritional factors in soybean, wheat bran is easily destroyed the microorganism structure of live pig enteron aisle, easily causes life Pig indigestion reduces weightening, and it is sick that severe patient results even in live pig.
Invention content
The purpose of the present invention is to provide with improve immune performance feedstuff for live pigs, feedstuff for live pigs nutrition comprehensive and abundant, There is luring function and enhancing live pig immune performance simultaneously, live pig lipid structure can be effectively improved, enhance live pig Constitution.
Used microorganism and its bought enterprise are in the present invention：Bacillus subtilis, the Cangzhou City macro animal of benefit protect Jian Pin Co., Ltds.
The problem of present invention in background technology for mentioning, the technical solution taken is：With the life for improving immune performance Pannage, the composition and its parts by weight of feedstuff for live pigs are：100-120 parts of fermented bean dregs, 3-5 parts of Meretrix meretrix Glycopeptide, fish oil nano-emulsion 2-3 parts, 1-2 parts of Chinese medicine preparation, 0.1-0.2 parts of Yolk antibody, 0.2-0.3 parts of sodium butyrate, 1.2-1.5 parts of carrotene, sulfa powder 0.1-0.2 parts, it is 3-4 parts of chitosan, 10-20 parts of sodium chloride, 0.5-0.6 parts of multidimensional, ore deposit 0.6-0.8 parts more；Feed contains abundant The nutriments such as protein, amino acid, microorganism, minerals, live pig can be effectively promoted and ingested, accelerate live pig growth, Feedstuff-meat ratio is reduced, reduces feeding cost, feed can also be effectively improved the immunocompetence and anti-stress ability of live pig, reduce by raising The harmful intestinal tract bacterial content that material strip comes, while the lipid structure of live pig can be improved, it is a kind of efficient raising immune performance Feedstuff for live pigs.
Feedstuff for live pigs with raising immune performance, including：The preparation of Meretrix meretrix Glycopeptide, prepares fish oil nanometer at bean pulp fermentation Breast, the preparation of Chinese medicine preparation, compounding, specifically include following steps：
The preparation of Meretrix meretrix Glycopeptide：Clam is taken to obtain clam dry powder after spitting sand, decladding, freeze-drying, crushing, by solid-liquid ratio 1:8-10 is molten It in distilled water and is homogenized, is centrifuged 25-30 minutes in 1-4 DEG C, 1000-2000r/min, collect supernatant, then surpass through doughnut Filter column ultrafiltration, ultrafiltration column molecular cut off are 8 × 104, small-molecular-weight ultrafiltrate is collected in ultrafiltration, in -20 ~ -40 DEG C of preservations；Super The chloroform of 1/5-2/5 volumes and the n-butanol of 1/25-1/20 volumes are added in filtrate, is extracted 3-4 times；Extract liquor is dialysed, from Obtain Meretrix meretrix Glycopeptide crude product after the heart, vacuum drying, centrifuging and taking supernatant after crude product is water-soluble, then with 0.1mol/L, 0.2mol/L, 0.4mol/L, 0.6mol/L, 0.8mol/L, 1.0mol/L sodium chloride solution gradient elution, obtain Meretrix meretrix Glycopeptide；Gained Meretrix meretrix Glycopeptide Purity be more than 96%, sugar and protein content each 54% and 42%, Meretrix meretrix Glycopeptide have excellent antitumor activity, antibacterial anti- Bacterium activity can effectively enhance the immunocompetence of live pig, enhance live pig constitution and vigor；
Bean pulp fermentation：45-48 parts of dregs of beans, 35-38 portions of corn flour, 10-12 parts of glucose, 10-12 parts of wheat bran powder are taken, according to Material-water ratio 1:2-3 adds in water, the bacillus subtilis seed liquor into 10-13% is inoculated with after stirring evenly, while add in withered grass gemma The tributyl 2-acetylcitrate of bacillus seed liquor weight 0.3-0.5 ‰ ferments 6-8 hours at a temperature of 36-37 DEG C, low after fermentation Temperature dries to obtain fermented bean dregs corn flour；Tributyl 2-acetylcitrate can generate the tricarboxylic acid cycle of bacillus subtilis beneficial Effect enhances its tricarboxylic acid cycle operational efficiency, and then improves the energy conversion efficiency of microorganism, improves bacillus subtilis Enzymatic activity greatly improves fermentation efficiency, reduces time cost；Through everfermentation, urase, trypsase in dregs of beans, wheat bran inhibit The content of the anti-nutritional factors such as the factor, aflatoxin substantially reduces, and the high molecular weight protein in raw material is fermented and is hydrolyzed to The nutriments such as micromolecule polypeptide, amino acid are suitble to being absorbed and utilized for live pig；
Prepare fish oil nano-emulsion：Fish oil and whey protein isolate are weighed respectively, dissolve breast with the phosphate buffer of pH7.0-7.2 Clear protein isolate prepares the whey protein isolate solution of 25-28g/L, after adding in 95-100g/L fish oil while stirring, in 4-10 At a temperature of DEG C thick lotion is obtained in 20000-25000r/min speed conditions for emulsified 100-120 seconds through high-speed shearing emulsion machine, it will Thick lotion recycle homogenization under 80-120MPa pressure and obtains fish oil nano-emulsion 4-5 times；Fish oil can be carried significantly through nano-emulsion embedding High fish oil oxidation stability, HIGH PRESSURE TREATMENT are conducive to improve the stability of fish oil nano-emulsion, and fish oil nano-emulsion has live pig strong Strong attractant effect, while the unrighted acid in fish oil can be effectively improved the lipid structure of live pig, enhance the body of live pig Matter reduces feedstuff-meat ratio；
The preparation of Chinese medicine preparation：Weigh 10-12 parts of radix scutellariaes, 3-4 portions of Chinese galls, 3-4 parts of Enteromorphas, 10-15 portions of cloves, 2-5 parts of jade Bamboo, 5-8 part Radix Astragali, 10-12 parts of Herba Epimedii smash it through 120-160 mesh sieve, traditional Chinese medicine powder are taken to add water to cook 60-90 minutes, natural Take supernatant concentration to ointment up to Chinese medicine preparation after cooling；Chinese medicine preparation safety, low toxicity, low-residual, are not likely to produce drug resistance, Tonifying spleen, stomach invigorating, tonifying Qi, solid table, adjust metabolism, enhance spleen and stomach function, can reduce stress reaction, the incidence of live pig, improve Immunocompetence and survival rate, while Chinese medicine preparation also has stronger luring function, live pig can be promoted the more to ingest, enhancing is raised Support efficiency；
Compounding：Weigh 100-120 parts of fermented bean dregs, 3-5 parts of Meretrix meretrix Glycopeptides, 2-3 parts of fish oil nano-emulsions, 1-2 parts of Chinese medicine preparations, 0.1-0.2 parts of Yolk antibodies, 0.2-0.3 parts of sodium butyrates, 1.2-1.5 parts of carrotene, 0.1-0.2 parts of sulfa powders, 3-4 parts of shells gather Sugar, 10-20 parts of sodium chloride, 0.5-0.6 parts of multidimensional, 0.6-0.8 parts of more ore deposits, crush after mixing, cross 30-40 mesh and sieve to have It is improved the feedstuff for live pigs of immune performance；Feed can be effectively improved the immunocompetence and anti-stress ability of live pig, reduce by raising The harmful intestinal tract bacterial content that material strip comes, while the lipid structure of live pig can be improved, feed is also containing abundant protein, amino The nutriments such as acid, microorganism, minerals, while live pig immune performance is improved can effectively facilitate live pig ingests, and accelerates Live pig grows up, and reduces feedstuff-meat ratio, reduces feeding cost.
Preferably, multidimensional used is VA or VB1Or VB2Or VB6Or VB12Or VC or VD or VE or VK or calcium pantothenate or At least five kinds of mixture of niacin or folic acid or biotin or inositol or microcrystalline cellulose；Multidimensional can improve the antibacterial of body Disease resistance improves immunocompetence, stress ability.
Preferably, more ore deposits used are MgSO4·7H2O and CuSO4·5H2O and FeSO4·H2O and ZnSO4·H2O with MnSO4·H2O and CoCl2·H2O and Ca (IO3)2With Na2SeO3Mixture；More ore deposits are supplemented for body necessary to growth and development Middle trace element.
Compared with prior art, the advantage of the invention is that：
1）In the fermentation step of dregs of beans and corn flour, tributyl 2-acetylcitrate can be to the tricarboxylic acid cycle of bacillus subtilis Beneficial effect is generated, enhances its tricarboxylic acid cycle operational efficiency, and then improve the energy conversion efficiency of microorganism, improves withered grass bud The enzymatic activity of spore bacillus greatly improves fermentation efficiency, reduces time cost；
2）Through everfermentation, the anti-nutritional factors such as urase, trypsin ihhibitor, aflatoxin in dregs of beans, wheat bran contain Amount substantially reduces, and the high molecular weight protein in raw material is fermented and is hydrolyzed to the nutriments such as micromolecule polypeptide, amino acid, is suitble to Live pig is absorbed and utilized；
3）Feed can be effectively improved the immunocompetence and anti-stress ability of live pig, reduce and contained by the harmful intestinal tract bacteria that feed is brought Amount, while the lipid structure of live pig can be improved, feed is also containing battalion such as abundant protein, amino acid, microorganism, minerals Substance is supported, can effectively facilitate live pig while live pig immune performance is improved ingests, and accelerates live pig growth, reduces feedstuff-meat ratio, Reduce feeding cost.
Specific embodiment
The present invention program is described further below by embodiment：
Embodiment 1：
With the feedstuff for live pigs for improving immune performance, the composition and its parts by weight of feedstuff for live pigs are：100 parts of fermented bean dregs, clam 3 parts of glycopeptide, 2 parts of fish oil nano-emulsion, 1 part of Chinese medicine preparation, 0.1 part of Yolk antibody, 0.2 part of sodium butyrate, 1.2 parts of carrotene, sulfanilamide (SN) 0.1 part of powder, 3 parts of chitosan, 10 parts of sodium chloride, 0.5 part of multidimensional, 0.6 part of more ore deposits；Feed contain abundant protein, amino acid, The nutriments such as microorganism, minerals can be effectively promoted live pig and ingest, and accelerate live pig growth, reduce feedstuff-meat ratio, reduce and raise This is formed, feed can also be effectively improved the immunocompetence and anti-stress ability of live pig, reduce the harmful intestinal tract brought by feed Bacterial content, while the lipid structure of live pig can be improved, it is a kind of efficient feedstuff for live pigs for improving immune performance.
Embodiment 2：
With the feedstuff for live pigs for improving immune performance, include the following steps：
The preparation of Meretrix meretrix Glycopeptide：Clam is taken to obtain clam dry powder after spitting sand, decladding, freeze-drying, crushing, by solid-liquid ratio 1:8 are dissolved in It in distilled water and is homogenized, is centrifuged 25 minutes in 1 DEG C, 1000r/min, collect supernatant, then through Hollow Fiber Ultrafiltration column ultrafiltration, ultrafiltration Column molecular cut off is 8 × 104, small-molecular-weight ultrafiltrate is collected in ultrafiltration, in -20 DEG C of preservations；1/5 volume is added in ultrafiltrate Chloroform and 1/25 volume n-butanol, extract 3 times；Extract liquor is dialysed, is centrifuged, be dried in vacuo after to obtain Meretrix meretrix Glycopeptide thick Product, centrifuging and taking supernatant after crude product is water-soluble, then with 0.1mol/L, 0.2mol/L, 0.4mol/L, 0.6mol/L, 0.8mol/L, 1.0mol/L sodium chloride solution gradient elutions, obtain Meretrix meretrix Glycopeptide；The purity of gained Meretrix meretrix Glycopeptide is more than 96%, and sugar and protein contain Each 54% and 42% is measured, Meretrix meretrix Glycopeptide has excellent antitumor activity, bacteriostasis antibiosis activity, can effectively enhance exempting from for live pig Epidemic disease ability enhances live pig constitution and vigor；
Bean pulp fermentation：45 parts of dregs of beans, 35 portions of corn flour, 10 parts of glucose, 10 parts of wheat bran powder are taken, according to material-water ratio 1:2 add Enter water, the bacillus subtilis seed liquor into 10% is inoculated with after stirring evenly, while add in bacillus subtilis seed liquor weight 0.3 ‰ tributyl 2-acetylcitrate ferments 6 hours at a temperature of 36 DEG C, and low temperature drying obtains fermented bean dregs corn flour after fermentation； Tributyl 2-acetylcitrate can generate beneficial effect to the tricarboxylic acid cycle of bacillus subtilis, enhance its tricarboxylic acid cycle fortune Line efficiency, and then the energy conversion efficiency of microorganism is improved, the enzymatic activity of bacillus subtilis is improved, greatly improves fermentation effect Rate reduces time cost；Through everfermentation, the anti-battalion such as urase, trypsin ihhibitor, aflatoxin in dregs of beans, wheat bran The content for supporting the factor substantially reduces, and the high molecular weight protein in raw material is fermented and is hydrolyzed to the battalion such as micromolecule polypeptide, amino acid Substance is supported, is suitble to being absorbed and utilized for live pig；
Prepare fish oil nano-emulsion：Fish oil and whey protein isolate are weighed respectively, with the phosphate buffer dissolving whey point of pH7.0 From albumen, the whey protein isolate solution of 25g/L is prepared, after adding in 95g/L fish oil while stirring, through at a high speed at a temperature of 4 DEG C Emulsification pretreatment machine obtains thick lotion in emulsified 100 seconds in 20000r/min speed conditions, and thick lotion is recycled under 80MPa pressure Matter handles 4 times to obtain fish oil nano-emulsion；Fish oil is remarkably improved fish oil oxidation stability through nano-emulsion embedding, and HIGH PRESSURE TREATMENT is conducive to The stability of fish oil nano-emulsion is improved, fish oil nano-emulsion has live pig strong attractant effect, while the unsaturation in fish oil Aliphatic acid can be effectively improved the lipid structure of live pig, enhance the constitution of live pig, reduce feedstuff-meat ratio；
The preparation of Chinese medicine preparation：Weigh 10 parts of radix scutellariaes, 4 portions of Chinese galls, 3 parts of Enteromorphas, 10 portions of cloves, 2 portions of radix polygonati officinalis, 5 parts of Radix Astragalis, 10 Part Herba Epimedii smashes it through 120 mesh sieve, traditional Chinese medicine powder is taken to add water to cook 60 minutes, supernatant concentration is taken after natural cooling to ointment Up to Chinese medicine preparation；Chinese medicine preparation safety, low toxicity, low-residual, are not likely to produce drug resistance, and tonifying spleen, stomach invigorating, tonifying Qi, solid table are adjusted Metabolism enhances spleen and stomach function, can reduce stress reaction, the incidence of live pig, improves immunocompetence and survival rate, while Chinese medicine Preparation also has stronger luring function, live pig can be promoted the more to ingest, and enhances feeding efficiency；
Compounding：110 parts of fermented bean dregs, 4 parts of Meretrix meretrix Glycopeptides, 2 parts of fish oil nano-emulsions, 1 part of Chinese medicine preparation, 0.15 part of yolk is weighed to resist Body, 0.2 part of sodium butyrate, 1.4 parts of carrotene, 0.15 part of sulfa powder, 3 parts of chitosans, 15 parts of sodium chloride, 0.1 part of VB12, 0.1 part VC, 0.1 part of VD, 0.1 part of VE, 0.1 part of VK, ore deposit more than 0.6 part, crush after mixing, cross 30 mesh and sieve that there is raising to be immunized The feedstuff for live pigs of performance；Feed can be effectively improved the immunocompetence and anti-stress ability of live pig, reduce the intestines brought by feed Road is harmful to bacterial content, while can improve the lipid structure of live pig, feed also containing abundant protein, amino acid, microorganism, The nutriments such as minerals, while live pig immune performance is improved can effectively facilitate live pig ingests, and accelerates live pig growth, drop Low feedstuff-meat ratio reduces feeding cost.
Embodiment 3：
With the feedstuff for live pigs for improving immune performance, the composition and its parts by weight of feedstuff for live pigs are：120 parts of fermented bean dregs, clam 5 parts of glycopeptide, 3 parts of fish oil nano-emulsion, 2 parts of Chinese medicine preparation, 0.2 part of Yolk antibody, 0.3 part of sodium butyrate, 1.5 parts of carrotene, sulfanilamide (SN) 0.2 part of powder, 4 parts of chitosan, 20 parts of sodium chloride, 0.6 part of multidimensional, 0.8 part of more ore deposits；Feed contain abundant protein, amino acid, The nutriments such as microorganism, minerals can be effectively promoted live pig and ingest, and accelerate live pig growth, reduce feedstuff-meat ratio, reduce and raise This is formed, feed can also be effectively improved the immunocompetence and anti-stress ability of live pig, reduce the harmful intestinal tract brought by feed Bacterial content, while the lipid structure of live pig can be improved, it is a kind of efficient feedstuff for live pigs for improving immune performance.
Feedstuff for live pigs with raising immune performance, including：The preparation of Meretrix meretrix Glycopeptide, prepares fish oil nanometer at bean pulp fermentation Breast, the preparation of Chinese medicine preparation, compounding, specifically include following steps：
The preparation of Meretrix meretrix Glycopeptide：Clam is taken to obtain clam dry powder after spitting sand, decladding, freeze-drying, crushing, by solid-liquid ratio 1:10 are dissolved in It in distilled water and is homogenized, is centrifuged 30 minutes in 4 DEG C, 2000r/min, collect supernatant, then through Hollow Fiber Ultrafiltration column ultrafiltration, ultrafiltration Column molecular cut off is 8 × 104, small-molecular-weight ultrafiltrate is collected in ultrafiltration, in -40 DEG C of preservations；2/5 volume is added in ultrafiltrate Chloroform and 1/20 volume n-butanol, extract 3-4 times；Extract liquor is dialysed, is centrifuged, be dried in vacuo after to obtain Meretrix meretrix Glycopeptide thick Product, centrifuging and taking supernatant after crude product is water-soluble, then with 0.1mol/L, 0.2mol/L, 0.4mol/L, 0.6mol/L, 0.8mol/L, 1.0mol/L sodium chloride solution gradient elutions, obtain Meretrix meretrix Glycopeptide；The purity of gained Meretrix meretrix Glycopeptide is more than 96%, and sugar and protein contain Each 54% and 42% is measured, Meretrix meretrix Glycopeptide has excellent antitumor activity, bacteriostasis antibiosis activity, can effectively enhance exempting from for live pig Epidemic disease ability enhances live pig constitution and vigor；
Bean pulp fermentation：48 parts of dregs of beans, 38 portions of corn flour, 12 parts of glucose, 12 parts of wheat bran powder are taken, according to material-water ratio 1:3 add Enter water, the bacillus subtilis seed liquor into 13% is inoculated with after stirring evenly, while add in bacillus subtilis seed liquor weight 0.5 ‰ tributyl 2-acetylcitrate ferments 8 hours at a temperature of 37 DEG C, and low temperature drying obtains fermented bean dregs corn flour after fermentation； Tributyl 2-acetylcitrate can generate beneficial effect to the tricarboxylic acid cycle of bacillus subtilis, enhance its tricarboxylic acid cycle fortune Line efficiency, and then the energy conversion efficiency of microorganism is improved, the enzymatic activity of bacillus subtilis is improved, greatly improves fermentation effect Rate reduces time cost；Through everfermentation, the anti-battalion such as urase, trypsin ihhibitor, aflatoxin in dregs of beans, wheat bran The content for supporting the factor substantially reduces, and the high molecular weight protein in raw material is fermented and is hydrolyzed to the battalion such as micromolecule polypeptide, amino acid Substance is supported, is suitble to being absorbed and utilized for live pig；
Prepare fish oil nano-emulsion：Fish oil and whey protein isolate are weighed respectively, with the phosphate buffer dissolving whey point of pH7.2 From albumen, the whey protein isolate solution of 28g/L is prepared, after adding in 100g/L fish oil while stirring, through height at a temperature of 10 DEG C Fast emulsification pretreatment machine obtains thick lotion in emulsified 120 seconds in 25000r/min speed conditions, and thick lotion is followed under 120MPa pressure Ring homogenization 5 times fish oil nano-emulsion；Fish oil is remarkably improved fish oil oxidation stability through nano-emulsion embedding, and HIGH PRESSURE TREATMENT has Conducive to the stability for improving fish oil nano-emulsion, fish oil nano-emulsion has strong attractant effect, while in fish oil not to live pig Saturated fatty acid can be effectively improved the lipid structure of live pig, enhance the constitution of live pig, reduce feedstuff-meat ratio；
The preparation of Chinese medicine preparation：Weigh 12 parts of radix scutellariaes, 4 portions of Chinese galls, 4 parts of Enteromorphas, 15 portions of cloves, 5 portions of radix polygonati officinalis, 8 parts of Radix Astragalis, 12 Part Herba Epimedii smashes it through 160 mesh sieve, traditional Chinese medicine powder is taken to add water to cook 90 minutes, supernatant concentration is taken after natural cooling to ointment Up to Chinese medicine preparation；Chinese medicine preparation safety, low toxicity, low-residual, are not likely to produce drug resistance, and tonifying spleen, stomach invigorating, tonifying Qi, solid table are adjusted Metabolism enhances spleen and stomach function, can reduce stress reaction, the incidence of live pig, improves immunocompetence and survival rate, while Chinese medicine Preparation also has stronger luring function, live pig can be promoted the more to ingest, and enhances feeding efficiency；
Compounding：120 parts of fermented bean dregs, 5 parts of Meretrix meretrix Glycopeptides, 3 parts of fish oil nano-emulsions, 2 parts of Chinese medicine preparations, 0.2 part of yolk is weighed to resist Body, 0.3 part of sodium butyrate, 1.2-1.5 parts of carrotene, 0.2 part of sulfa powder, 4 parts of chitosans, 20 parts of sodium chloride, 0.1 part of calcium pantothenate, 0.1 part of niacin, 0.1 part of folic acid, 0.1 part of biotin, 0.1 part of inositol, 0.1 part of microcrystalline cellulose, ore deposit more than 0.8 part are uniformly mixed After crush, cross 40 mesh sieve up to have improve immune performance feedstuff for live pigs；Feed can be effectively improved the immunocompetence of live pig With anti-stress ability, the harmful intestinal tract bacterial content brought by feed is reduced, while the lipid structure of live pig can be improved, feed is also It, can be with while live pig immune performance is improved containing nutriments such as abundant protein, amino acid, microorganism, minerals It effectively facilitates live pig to ingest, accelerates live pig growth, reduce feedstuff-meat ratio, reduce feeding cost.
More ore deposits are MgSO4·7H2O and CuSO4·5H2O and FeSO4·H2O and ZnSO4·H2O and MnSO4·H2O with CoCl2·H2O and Ca (IO3)2With Na2SeO3Mixture；Middle trace element necessary to more ore deposits supplement growth and development for body.
Routine operation in operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme of the present invention is described in detail in embodiment described above, it should be understood that the above is only For specific embodiments of the present invention, it is not intended to restrict the invention, all any modifications made in the spirit of the present invention, Supplement or similar fashion replacement etc., should all be included in the protection scope of the present invention.

Claims (9)

1. with the feedstuff for live pigs for improving immune performance, it is characterised in that：The composition and its parts by weight of the feedstuff for live pigs be：Hair 100-120 parts of ferment dregs of beans, 3-5 parts of Meretrix meretrix Glycopeptide, 2-3 parts of fish oil nano-emulsion, 1-2 parts of Chinese medicine preparation, Yolk antibody 0.1-0.2 Part, 0.2-0.3 parts of sodium butyrate, 1.2-1.5 parts of carrotene, 0.1-0.2 parts of sulfa powder, 3-4 parts of chitosan, sodium chloride 10-20 It is part, 0.5-0.6 parts of multidimensional, ore deposit 0.6-0.8 parts more.
2. according to claim 1 have the feedstuff for live pigs for improving immune performance, it is characterised in that：It is described that there is raising to exempt from The preparation method of the feedstuff for live pigs of epidemic disease performance includes：The preparation of Meretrix meretrix Glycopeptide, prepares fish oil nano-emulsion, Chinese medicine system at bean pulp fermentation The preparation of agent, compounding, the bean pulp fermentation step are：Take 45-48 parts of dregs of beans, 35-38 portions of corn flour, 10-12 parts of glucose, 10-12 parts of wheat bran powder, according to material-water ratio 1:2-3 adds in water, and the bacillus subtilis into 10-13% is inoculated with after stirring evenly Seed liquor adds in the tributyl 2-acetylcitrate of bacillus subtilis seed liquor weight 0.3-0.5 ‰, and low temperature drying obtains after fermentation Fermented bean dregs corn flour.
3. according to claim 2 have the feedstuff for live pigs for improving immune performance, it is characterised in that：The bean pulp fermentation step Fermentation temperature in rapid is 36-37 DEG C, and fermentation time is 6-8 hours.
4. according to claim 2 have the feedstuff for live pigs for improving immune performance, it is characterised in that：The Meretrix meretrix Glycopeptide Preparation process is：Clam is taken to obtain clam dry powder after spitting sand, decladding, freeze-drying, crushing, by solid-liquid ratio 1:8-10 is dissolved in distilled water In and be homogenized, centrifuged 25-30 minute in 1-4 DEG C, 1000-2000r/min, collection supernatant, then through Hollow Fiber Ultrafiltration column ultrafiltration, Ultrafiltration column molecular cut off is 8 × 104, small-molecular-weight ultrafiltrate is collected in ultrafiltration, in -20 ~ -40 DEG C of preservations；Add in ultrafiltrate Enter the chloroform of 1/5-2/5 volumes and the n-butanol of 1/25-1/20 volumes, extract 3-4 times；Extract liquor is dialysed, is centrifuged, vacuum is done Obtain Meretrix meretrix Glycopeptide crude product after dry, centrifuging and taking supernatant after crude product is water-soluble, then with 0.1mol/L, 0.2mol/L, 0.4mol/L, 0.6mol/L, 0.8mol/L, 1.0mol/L sodium chloride solution gradient elution, obtain Meretrix meretrix Glycopeptide.
5. according to claim 4 have the feedstuff for live pigs for improving immune performance, it is characterised in that：The Meretrix meretrix Glycopeptide The additive amount of chloroform is the 1/5-2/5 of ultrafiltrate volume in preparation process, and the additive amount of n-butanol is the 1/25- of ultrafiltrate volume 1/20。
6. according to claim 2 have the feedstuff for live pigs for improving immune performance, it is characterised in that：The fish oil for preparing is received Rice milk step is：Fish oil and whey protein isolate are weighed respectively, and egg is detached with the phosphate buffer dissolving whey of pH7.0-7.2 In vain, the whey protein isolate solution of 25-28g/L is prepared, 95-100g/L fish oil is added in while stirring, through high-speed shearing emulsion machine Emulsify to obtain thick lotion, thick lotion is recycled under 80-120MPa pressure homogenization 4-5 times fish oil nano-emulsion.
7. according to claim 6 have the feedstuff for live pigs for improving immune performance, it is characterised in that：The fish oil for preparing is received Emulsifying temperature in rice milk step is 4-10 DEG C, and the rotating speed of high-speed shearing emulsion machine is 20000-25000r/min, emulsification times It is 100-120 seconds.
8. according to claim 2 have the feedstuff for live pigs for improving immune performance, it is characterised in that：The Chinese medicine preparation It prepares：Weigh 10-12 parts of radix scutellariaes, 3-4 portions of Chinese galls, 3-4 parts of Enteromorphas, 10-15 portions of cloves, 2-5 portions of radix polygonati officinalis, 5-8 parts of Radix Astragalis, 10-12 parts of Herba Epimedii smash it through 120-160 mesh sieve, traditional Chinese medicine powder are taken to add water to cook 60-90 minutes, supernatant is taken after natural cooling Liquid is concentrated into ointment up to Chinese medicine preparation.
9. according to claim 2 have the feedstuff for live pigs for improving immune performance, it is characterised in that：The compounding step For：Weigh 100-120 parts of fermented bean dregs, 3-5 parts of Meretrix meretrix Glycopeptides, 2-3 parts of fish oil nano-emulsions, 1-2 parts of Chinese medicine preparations, 0.1-0.2 Part Yolk antibody, 0.2-0.3 parts of sodium butyrates, 1.2-1.5 parts of carrotene, 0.1-0.2 parts of sulfa powders, 3-4 parts of chitosans, 10-20 Part sodium chloride, 0.5-0.6 parts of multidimensional, 0.6-0.8 parts of more ore deposits, crush after mixing, cross 30-40 mesh and sieve that there is raising to exempt from The feedstuff for live pigs of epidemic disease performance.
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