CN108168958A - A kind of method for collecting Xylem sap - Google Patents
A kind of method for collecting Xylem sap Download PDFInfo
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- CN108168958A CN108168958A CN201711427675.4A CN201711427675A CN108168958A CN 108168958 A CN108168958 A CN 108168958A CN 201711427675 A CN201711427675 A CN 201711427675A CN 108168958 A CN108168958 A CN 108168958A
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- sap
- bleeding
- emgloves
- bleeding sap
- silica gel
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/20—Devices for withdrawing samples in the liquid or fluent state for flowing or falling materials
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
- G01N2001/1445—Overpressure, pressurisation at sampling point
- G01N2001/1463—Injector; Air-lift
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Abstract
The invention discloses a kind of methods for collecting Xylem sap, bleeding sap is mainly collected using a kind of easy bleeding liquor collecting device, specifically intercept one section of transparent silica gel hose, select a secondary emgloves, the middle finger portion of emgloves along middle finger is referred to scissors and is cut at root, the emgloves middle finger portion cut is cut apart from the position of 0.5 1cm of finger tip, then one end of silica gel hose is inserted into the narrow one end of emgloves middle finger portion for cutting finger tip, it is pasted securely with glue again, the other end of silica gel hose, which knots, to be closed；Compared with prior art, the present invention simple to operation, field can quick obtaining bleeding sap volume, weight, there is the advantages of collection efficiency is fast, and organic efficiency is high.
Description
Technical field
The present invention relates to bleeding sap collection technique fields, and in particular to a kind of method for collecting Xylem sap.
Background technology
Root system is the vitals of plant absorption nutrient and moisture, and in plant growth and development process, xylem is root system
The important channel of nutrient and moisture is transported to overground part, when aboveground vegetation part is cut, the liquid flowed out from xylem is just
Referred to as bleeding sap.The amount and active material of Xylem sap can usually be used to refer to root system of plant energy level.So it is
The size of clear and definite root activity, the collection method of bleeding sap are most important.
There are mainly two types of the collection methods of currently used bleeding sap, the first is volumetric method, and bleeding is collected with volumetric method
During liquid, due to meeting obstructions when bleeding sap passes through rubber tube and glass tube, in the plant growth later stage, it is generally difficult to be collected into enough
Bleeding sap, have certain limitation；Second is gravimetric absorption method, and such method needs fill up elastic suitable in glass tube
In drying defatted cotton, and need the absorbent cotton in glass tube and plant shoot notch being in close contact, operationally very
Difficult control, after absorbent cotton absorbs complete bleeding sap, it is also necessary to centrifuge absorbent cotton testing indoor centrifuge, the supernatant of acquisition
On the one hand liquid, as bleeding sap, this method need to coordinate indoor centrifuge that could obtain complete bleeding sap, operating process consumption
Duration is unfavorable for the collection of field bleeding sap, and on the other hand easily there are absorbent cotton in the bleeding sap of collection, pollute.
Other than both the above method, there is such as micro-damage bast to collect the method for bleeding sap and pressurize at present and received with negative pressure
Collection method etc., micro-damage bast collecting method are only applicable to the dicotyledons such as cotton, and for monocotyledons such as picture corns simultaneously
Inapplicable, the scope of application is small；Pressurization and negative pressure collecting method, the equipment on the one hand needed is comparatively laborious, and efficiency is very low, the opposing party
Face, when carrying out quantitative study, the technology requirement of pressurization and negative pressure is higher.
Invention content
The purpose of the present invention is to provide a kind of methods for collecting Xylem sap, and this method is simple to operation, to
Solve in the prior art Xylem sap collect existing for limitation, complexity, it is cumbersome and easy to pollute the problems such as.
To achieve the above object, the technical scheme is that：
A kind of method for collecting Xylem sap, described method includes following steps：
(1) bleeding liquor collecting device is prepared：The transparent silica gel hose of one section of long 30-35cm is intercepted, selects a secondary latex hand
Set, the middle finger portion of emgloves is referred to cut at root with scissors along middle finger, the emgloves middle finger portion distance that will be cut
The position of finger tip 0.5-1cm is cut, then one end of transparent silica gel hose is inserted into the emgloves middle finger portion for cutting finger tip
Narrow one end, intubating length 2-3cm, then pasted securely with 502 glue, the other end of transparent silica gel hose, which knots, to be sealed
It closes；
(2) centrifuge tube weight is weighed：Centrifuge tube is taken, the weight of centrifuge tube is weighed with 1 percent balances, and records centrifugation
The weight W1 of pipe；
(3) bleeding liquor collecting device is installed：Uniformly representative plant is chosen, at 2-3cm from the ground, is cut with knife
Plant shoot is removed, section is rinsed well with deionized water, and blotted moisture with absorbent cotton after 1-2min, then existed rapidly
Install the bleeding liquor collecting device prepared at section, specific installation method be by emgloves middle finger portion it is wide one
End cap is fixed at plant section, and with rubber band pitch of the laps on plant, and the one end for the closing that knots is placed on the ground；
(4) bleeding sap is collected：By the collection of 2-24 hours, determine that the bleeding sap in collection device no longer increases, note
The syringe needle of emitter is inserted into the part that bleeding sap is assembled in transparent silica gel hose, the bleeding sap in transparent silica gel hose is sucked out, so
The syringe needle of syringe is inserted into the emgloves middle finger portion at plant section again afterwards, by wound remaining in emgloves
Flow liquid is sucked out, and according to the scale on syringe, obtains the volume of bleeding sap, is then transferred to the bleeding sap in syringe
In the centrifuge tube weighed；Rubber band is removed, removes collection device, that is, completes the collection process of entire bleeding sap；
(5) bleeding sap weight is obtained：In field, the centrifuge tube equipped with bleeding sap is weighed with 1 percent balances, is recorded
For W2, W2-W1 is the weight of bleeding sap；
(6) bleeding sap stores：Bleeding sap after weighing in field is put into ice chest rapidly, and -20 are put into after transporting laboratory back
It is stored in DEG C refrigerator, in case the measure of physiological activity index.
Preferably, the internal diameter of the transparent silica gel hose is 10mm, outer diameter 15mm.
Wherein described centrifuge tube and syringe can select suitable range according to the amount of different plant bleeding saps, for jade
10ml centrifuge tubes and 10ml syringes are generally selected in the collection of rice bleeding sap.
Further, selected knife be also can according to it is required collect bleeding sap plant xylem tough and tensile situation
Depending on, for corn, wallpaper knife can be selected.
Further, for convenience's sake, the emgloves can be with selection criteria money milky L code disposable emulsions
Gloves.
It preferably, can be at knotting with the padded 1-2cm of absorbent cotton, to increase transparent silica gel in actual mechanical process
Hose pastes the contact area of bleeding sap in one end and emgloves of emgloves, so as to preferably promote bleeding sap outflow.
The method of the present invention has the following advantages that：
Compared with prior art, the present invention simple to operation, field can quick obtaining bleeding sap volume, again
Amount is breached in volumetric method due to meeting obstructions when bleeding sap passes through rubber tube and glass tube, it is difficult to be collected into enough bleeding saps
Bottleneck, avoid gravimetric absorption method and pressurization and negative pressure collecting method need to carry out the cumbersome of bleeding sap recycling indoors, have
The advantages of collection efficiency is fast, and organic efficiency is high.
Description of the drawings
Fig. 1 is bleeding liquor collecting device structure diagram；
In figure, 1- transparent silica gels hose, 2- emgloves middle finger portion, 3- plant, 4- rubber bands, 5- absorbent cotton.
Specific embodiment
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..
Embodiment 1
A kind of method for collecting corn Xylem sap, described method includes following steps：
(1) bleeding liquor collecting device (as shown in Figure 1) is prepared：The transparent silica gel hose 1 of one section of long 30cm is intercepted, it is described
The internal diameter of bright silica gel hose 1 is 10mm, outer diameter 15mm, a secondary standard money milky L code disposable emulsion gloves is selected, with cutting
Knife refers to emgloves middle finger portion 2 along middle finger to be cut at root, by the emgloves middle finger portion cut apart from finger tip 0.5cm
Position cut, then one end of transparent silica gel hose 1 is inserted into the narrow one end of emgloves middle finger portion for cutting finger tip, inserted
Enter length for 2cm, then pasted securely with 502 glue, the other end of transparent silica gel hose 1, which knots, to be closed；
(2) centrifuge tube weight is weighed：10ml centrifuge tubes are taken, the weight of centrifuge tube is weighed with 1 percent balances, and are recorded
The weight W1 of centrifuge tube；
(3) bleeding liquor collecting device is installed：Uniformly representative plant 3 is chosen, at 2cm from the ground, uses wallpaper
Knife cuts plant shoot, is rinsed well section with deionized water, and blotted moisture with absorbent cotton after 1min, then rapidly
The bleeding liquor collecting device prepared is installed at section, specific installation method is that emgloves middle finger portion is wide
One end is sleeved at plant section, and is fixed on plant with 4 pitch of the laps of rubber band, and the one end for the closing that knots is placed on the ground；
In actual mechanical process, with 5 padded 1cm of absorbent cotton or so at knotting, to promote the outflow of bleeding sap；
(4) bleeding sap is collected：By the collection of 2 hours, determine that the bleeding sap in collection device no longer increases, 10ml is noted
The syringe needle of emitter is inserted into the part that bleeding sap is assembled in transparent silica gel hose, the bleeding sap in transparent silica gel hose is sucked out, so
The syringe needle of syringe is inserted into the emgloves middle finger portion at plant section again afterwards, by wound remaining in emgloves
Flow liquid is sucked out, and according to the scale on syringe, obtains the volume of bleeding sap, is then transferred to the bleeding sap in syringe
In the centrifuge tube weighed, rubber band is removed, removes collection device, that is, completes the collection process of entire bleeding sap；
(5) bleeding sap weight is obtained：In field, the centrifuge tube equipped with bleeding sap is weighed with 1 percent balances, is recorded
For W2, W2-W1 is the weight of bleeding sap；
(6) bleeding sap stores：Bleeding sap after weighing in field is put into ice chest rapidly, and -20 are put into after transporting laboratory back
It is stored in DEG C refrigerator, in case the measure of physiological activity index.
Embodiment 2
A kind of method for collecting corn Xylem sap, described method includes following steps：
(1) bleeding liquor collecting device (as shown in Figure 1) is prepared：The transparent silica gel hose 1 of one section of long 35cm is intercepted, it is described
The internal diameter of bright silica gel hose 1 is 10mm, outer diameter 15mm, a secondary standard money milky L code disposable emulsion gloves is selected, with cutting
Knife refers to emgloves middle finger portion 2 along middle finger to be cut at root, is then inserted into one end of transparent silica gel hose and is cut finger tip
The narrow one end of emgloves middle finger portion, intubating length 3cm, then pasted securely with 502 glue, transparent silica gel hose
The other end knot closing；
(2) centrifuge tube weight is weighed：10ml centrifuge tubes are taken, the weight of centrifuge tube is weighed with 1 percent balances, and are recorded
The weight W1 of centrifuge tube；
(3) bleeding liquor collecting device is installed：Uniformly representative plant 3 is chosen, at 3cm from the ground, uses wallpaper
Knife cuts plant shoot, is rinsed well section with deionized water, and blotted moisture with absorbent cotton after 2min, then rapidly
The bleeding liquor collecting device prepared is installed at section, specific installation method is that emgloves middle finger portion is wide
One end is sleeved at plant section, and is fixed on plant with 4 pitch of the laps of rubber band, and the one end for the closing that knots is placed on the ground；
In actual mechanical process, with 5 padded 2cm of absorbent cotton or so at knotting, to promote the outflow of bleeding sap；
(4) bleeding sap is collected：By the collection of 3 hours, determine that the bleeding sap in collection device no longer increases, 10ml is noted
The syringe needle of emitter is inserted into the part that bleeding sap is assembled in transparent silica gel hose, the bleeding sap in transparent silica gel hose is sucked out, so
The syringe needle of syringe is inserted into the emgloves middle finger portion at plant section again afterwards, by wound remaining in emgloves
Flow liquid is sucked out, and according to the scale on syringe, obtains the volume of bleeding sap, is then transferred to the bleeding sap in syringe
In the centrifuge tube weighed；Rubber band is removed, removes collection device, that is, completes the collection process of entire bleeding sap；
(5) bleeding sap weight is obtained：In field, the centrifuge tube equipped with bleeding sap is weighed with 1 percent balances, is recorded
For W2, W2-W1 is the weight of bleeding sap；
(6) bleeding sap stores：Bleeding sap after weighing in field is put into ice chest rapidly, and -20 are put into after transporting laboratory back
It is stored in DEG C refrigerator, in case the measure of physiological activity index.
In order to further verify effectiveness of the invention, inventor has also carried out following experiment, specific as follows：
First, the method for the present invention is compared with gravimetric absorption method
In Maize at Seedling Stage, being chosen in field has uniform representative 12 plants of plant, and 6 plants of corns are collected using gravimetric absorption method
Bleeding sap at 2cm from the ground, cuts plant shoot with wallpaper knife, is rinsed well section with deionized water after 1-2min,
And blotted moisture with absorbent cotton, glass tube is put on immediately, absorbent cotton must be made to be in close contact with overground part notch, and glass tube will
One end is asked to close, one end open fills up elastic moderate drying defatted cotton, and weigh on balance (W1) in advance in pipe, passes through
The collection of 2 hours is crossed, removes glass tube, cleans pollutant and moisture in glass pipe outer wall, weigh (W2) on balance, twice
Weight only poor (W1-W2) is the weight of bleeding sap.Then the glass tube equipped with absorbent cotton is taken back into laboratory and carries out bleeding sap
Recycling, absorbent cotton is put into the centrifuge tube of 10ml, centrifuge tube is put into centrifuge, 10000r/min is set, is periodically 3 points
Clock, after centrifugation, it is bleeding sap to take supernatant.The method of remaining 6 plants of corns embodiment of the present invention 1 collects bleeding
Liquid.Then compare the bleeding sap rate of recovery of the two, be specifically shown in Table 1.
1 gravimetric absorption method of table and the method for the present invention bleeding sap rate of recovery contrast table
As it can be seen from table 1 using the rate of recovery of the method for the present invention corn bleeding sap be apparently higher than with gravimetric absorption method from
The obtained bleeding sap rate of recovery after the heart, and when plant bleeding sap is less, the rate of recovery of gravimetric absorption method substantially reduces, and
The method of the present invention, which needs not move through indoor centrifuge, can directly recycle bleeding sap, not only reduce operating procedure, and
And significantly improve the rate of recovery of bleeding sap.
2nd, the method for the present invention is compared with volumetric method
After corn silking 40 days after 20 days and spinning, chosen in field has uniform representative 12 plants, 6 plants of plant respectively
Bleeding sap is collected using volumetric method, at 3cm from the ground, plant shoot is cut with wallpaper knife, deionized water is used after 1-2min
Section is rinsed well, and is blotted moisture with absorbent cotton, the short rubber tube that diameter is suitble to is put on rapidly, water is filled in advance in pipe
Point, it is connected on the glass tube of exact scale, collects 3 hours, according to the difference of liquid level in glass tube, bleeding sap can be obtained
Volume.The method of remaining 6 plants of corns embodiment of the present invention 2 collects bleeding sap, then compares bleeding sap collecting amount.
2 volumetric method of table collects bleeding liquid measure contrast table with the method for the present invention
From table 2 it can be seen that 40 days after still spinning within 20 days either after corn silking, the wound that the method for the present invention is collected
Flow liquid amount is all apparently higher than the bleeding liquid measure collected with volumetric method, and 40 days especially after spinning, corn growth enters fast
The stage of fast aging has been difficult to regather bleeding sap with volumetric method, and the method for the present invention can also continue to collect it is a certain amount of
Bleeding sap, for judging the root activity state of this period plant.
Although above having used general explanation and specific embodiment, the present invention is described in detail, at this
On the basis of invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Therefore,
These modifications or improvements without departing from theon the basis of the spirit of the present invention belong to the scope of protection of present invention.
Claims (7)
- A kind of 1. method for collecting Xylem sap, which is characterized in that described method includes following steps：(1) bleeding liquor collecting device is prepared：The transparent silica gel hose of one section of long 30-35cm is intercepted, a secondary emgloves is selected, uses Scissors refers to the middle finger portion of emgloves along middle finger to be cut at root, by the emgloves middle finger portion cut apart from finger tip The position of 0.5-1cm is cut, and then emgloves middle finger portion that the insertion of one end of transparent silica gel hose is cut to finger tip is narrow One end, intubating length 2-3cm, then pasted securely with 502 glue, the other end of transparent silica gel hose, which knots, to be closed；(2) centrifuge tube weight is weighed：Centrifuge tube is taken, the weight of centrifuge tube is weighed with 1 percent balances, and records centrifuge tube Weight W1；(3) bleeding liquor collecting device is installed：Uniformly representative plant is chosen, at 2-3cm from the ground, plant is cut with knife Object overground part is rinsed well section with deionized water, and blotted moisture with absorbent cotton after 1-2min, then rapidly in section Place installs the bleeding liquor collecting device prepared, and specific installation method is by the wide end cap of emgloves middle finger portion It is fixed on plant at plant section, and with rubber band pitch of the laps, the one end for the closing that knots is placed on the ground；(4) bleeding sap is collected：By the collection of 2-24 hours, determine that the bleeding sap in collection device no longer increases, syringe Syringe needle be inserted into transparent silica gel hose in bleeding sap assemble part, by transparent silica gel hose bleeding sap be sucked out, Ran Houzai The syringe needle of syringe is inserted into the emgloves middle finger portion at plant section, by bleeding sap remaining in emgloves It is sucked out, according to the scale on syringe, obtains the volume of bleeding sap, be then transferred to the bleeding sap in syringe weighed Centrifuge tube in；(5) bleeding sap weight is obtained：In field, the centrifuge tube equipped with bleeding sap is weighed with 1 percent balances, is recorded as W2, W2-W1 is the weight of bleeding sap；(6) bleeding sap stores：Bleeding sap after weighing in field is put into ice chest rapidly, and -20 DEG C of ice are put into after transporting laboratory back It is stored in case, in case the measure of physiological activity index.
- 2. the method according to claim 1 for collecting Xylem sap, which is characterized in that the transparent silica gel hose Internal diameter is 10mm, outer diameter 1Smm.
- 3. the method according to claim 1 for collecting Xylem sap, which is characterized in that the centrifuge tube volume is 10ml, the syringe volume are 10ml.
- 4. the method according to claim 1 for collecting Xylem sap, which is characterized in that the knife is wallpaper knife.
- 5. the method according to claim 1 for collecting Xylem sap, which is characterized in that the emgloves is primary Property emgloves.
- 6. the method according to claim 5 for collecting Xylem sap, which is characterized in that the disposable emulsion gloves Using standard money milky L codes.
- 7. the method according to claim 1 for collecting Xylem sap, which is characterized in that with degreasing cotton pad at knotting High 1-2cm, to promote the outflow of bleeding sap.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711427675.4A CN108168958A (en) | 2017-12-26 | 2017-12-26 | A kind of method for collecting Xylem sap |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711427675.4A CN108168958A (en) | 2017-12-26 | 2017-12-26 | A kind of method for collecting Xylem sap |
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| CN108168958A true CN108168958A (en) | 2018-06-15 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109328975A (en) * | 2018-08-30 | 2019-02-15 | 江苏龙山湖生物科技有限公司 | A kind of minimally invasive acquisition device of fig living body bleeding sap |
| CN109646991A (en) * | 2019-01-15 | 2019-04-19 | 山西药科职业学院 | A kind of medicinal plant sap extraction device |
| CN109738589A (en) * | 2019-02-13 | 2019-05-10 | 南京禾稼春生物科技有限公司 | A kind of method of rapidly extracting plant xylem sap |
| CN110895213A (en) * | 2019-12-11 | 2020-03-20 | 天津农学院 | Sampling device for field rice bleeding sap |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109328975A (en) * | 2018-08-30 | 2019-02-15 | 江苏龙山湖生物科技有限公司 | A kind of minimally invasive acquisition device of fig living body bleeding sap |
| CN109646991A (en) * | 2019-01-15 | 2019-04-19 | 山西药科职业学院 | A kind of medicinal plant sap extraction device |
| CN109646991B (en) * | 2019-01-15 | 2021-08-03 | 山西药科职业学院 | Medicinal plant juice extractor |
| CN109738589A (en) * | 2019-02-13 | 2019-05-10 | 南京禾稼春生物科技有限公司 | A kind of method of rapidly extracting plant xylem sap |
| CN109738589B (en) * | 2019-02-13 | 2021-12-17 | 南京禾稼春生物科技有限公司 | Method for rapidly extracting xylem sap of plant |
| CN110895213A (en) * | 2019-12-11 | 2020-03-20 | 天津农学院 | Sampling device for field rice bleeding sap |
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Application publication date: 20180615 |