CN108165477A - A kind of Quick sterilizing device of microbial inoculant tool - Google Patents
A kind of Quick sterilizing device of microbial inoculant tool Download PDFInfo
- Publication number
- CN108165477A CN108165477A CN201810188994.2A CN201810188994A CN108165477A CN 108165477 A CN108165477 A CN 108165477A CN 201810188994 A CN201810188994 A CN 201810188994A CN 108165477 A CN108165477 A CN 108165477A
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- Prior art keywords
- tool
- sterilizing device
- microbial inoculant
- electrode
- energy
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Apparatus For Disinfection Or Sterilisation (AREA)
Abstract
The present invention discloses a kind of Quick sterilizing device of microbial inoculant tool, passes through transfer needle(Ring)Connecting rod contact bactericidal unit top electrode, electrode under wire contact bactericidal unit realizes transfer needle(Ring)It is formed into a loop with the storage capacitor of bactericidal unit, wire is under the action of high current and self-resistance, rapid fever, achieve the purpose that sterilizing, structure design of the present invention is simple, it is cost-effective, it can realize the automatic inflatable Electricity Functional of bactericidal unit, inoculating tool is with bactericidal unit without connection, sterilizing function is realized by contact, high efficiency and safety during the guarantee use of high degree, simultaneously, the lower electrode of bactericidal unit contact inoculation part can realize self-cleaning function, further improve the sterilization effect of bactericidal unit.
Description
Technical field
The present invention relates to microorganisms to connect instrument field, is filled more particularly, to a kind of quick sterilization of microbial inoculant tool
It puts.
Background technology
Common inoculating tool has transfer needle and oese, and transfer needle is commonly used in the puncture of fungi fungus block picking or bacterium
Inoculation, oese is usually used in the plate streaking of bacterium solution or bacterium colony, in Experiment on Microbiology operating process, it is often necessary to more than using
Alcolhol burner flame sterilization mode is commonly used in two kinds of inoculating tools, the sterilizing of inoculating tool, and alcolhol burner open fire temperature is by air stream
Dynamic influence and it is uneven, make disinfection not perfect, and alcolhol burner is easily overturned in operation, alcohol outflow causes danger, therefore,
How to provide a kind of safe, quick, effective and inoculating tool sterilizing methods easy to use becomes current urgent problem.
Notification number is that the patent of invention of 100354008 C of CN discloses a kind of electricity-burned type transfer needle disinfection apparatus, bottom
Control circuit is installed in seat, the heat-generating pipe of bracket end installation is connected by the control circuit in the conducting wire and pedestal in stent,
Metal bar end set heating wire, the control circuit connection in the metal contact piece and pedestal that set on base panel, by laboratory
With glassware as carrying out disinfection on heat-generating pipe, transfer needle contacts heating power with metal contact piece, realizes the disinfection of transfer needle.
But g and h circuits cause fulguration function that can not realize there are design defect in the circuit diagram 4 of the invention, and the invention is also deposited
Of high cost, need high-power transformer circuit complicated, and the diode of fulguration current loop setting is deposited in current state
The problems such as in very big useless power consumption so as to easily damage.
Invention content
The technical problem to be solved by the present invention is to:It overcomes the deficiencies of the prior art and provide a kind of using inoculation needle ring itself
Resistance, using farad grade capacitance as energy-storage travelling wave tube, makes heater moment burn red reach by high current fever as heater
The Quick sterilizing device of quick sterilization purpose microbial inoculant tool.
The technical solution adopted by the present invention to solve the technical problems is:
A kind of Quick sterilizing device of microbial inoculant tool, including transfer needle(Ring), support base, supporting rod, top electrode, lower electricity
Pole, energy-storage travelling wave tube, external power supply and control circuit, transfer needle are followed successively by insulated handle, metal tie rods and metal from top to bottom
Silk, support base and supporting rod are insulating materials, and lower electrode is arranged on support base upper surface, supporting rod connection top electrode and support
Seat, upper/lower electrode connect energy-storage travelling wave tube and control circuit by conducting wire, and extraneous power supply is connect with control circuit provides electric energy.Sterilizing
Before, extraneous power supply charges to energy-storage travelling wave tube by control circuit, and charging is automatically stopped after charging complete;During sterilizing, operation
Inoculating tool conductive portions contact upper/lower electrode makes inoculating tool be formed into a loop with energy-storage travelling wave tube and control circuit, energy-storage travelling wave tube
Electric discharge realizes fast heating using inoculating tool self-resistance, achievees the purpose that sterilizing;After the completion of sterilizing, inoculating tool is detached from
Upper/lower electrode, circuit disconnect, and energy-storage travelling wave tube discharge off is continued to charge to energy-storage travelling wave tube by control circuit, carry out next
Secondary sterilizing prepares.
Further, inoculating tool is transfer needle or oese, during sterilizing, transfer needle(Ring)Wire contact under electrode,
Metal tie rods part contacts top electrode.
Further, the energy-storage travelling wave tube is large capacity farad grade capacitance.
Further, microswitch is connected at the energy-storage travelling wave tube, realizes the manual boost charge function of energy-storage travelling wave tube.
Further, it is closing for embedded circular arc type that one end, which is made, by the stainless steel tube or copper pipe of diameter 3-6mm in the top electrode
Structure is closed, for top electrode with supporting the movable connection, top electrode position is adjustable.
Further, the lower electrode is the pellet resistance of 3*0.1mm, and length is in 50-60mm.
Further, it is described to power on extremely with connecting change-over switch or switching relay among energy-storage travelling wave tube, in lower electrode
Cleaning is needed to make, by circuit conversion, makes to be formed into a loop between capacitance and lower electrode, realizes the lower spontaneous thermal cleaning of electrode;Into
During row disinfecting action, change-over switch adjustment makes capacitance be formed into a loop with top electrode, lower electrode and inoculating tool, realizes inoculation work
The heat sterilization of tool.
Further, the control circuit is 555 time base circuits.
Further, the wire is made of platinum, nichrome or Aludirome, overall length be 30 ~ 60mm, diameter
For 0.5 ~ 1mm.
Further, the wire and metal tie rods are movable connection, wire for a long time using being lost,
It is movable connection with metal tie rods, can replaces at any time.
Further, the external power supply is 12V batteries, 12V Switching Power Supplies or the 12V DC electricity after transformer transformation
Source.
Beneficial effects of the present invention:The bactericidal unit of the present invention realizes that instantaneous large-current is sent out using the resistance of transfer needle itself
Heat, which is burnt, reaches instantaneously sterilising function;555 time base circuits can realize storage capacitor automatic charge control function;Its farad of grade storage
Energy capacitance can reduce the requirement to power supply while larger operating current is obtained;Lower electrode utilizes low voltage electric iron
Core, with reference to change-over switch, realizes the current limliting and automatically cleaning of lower electrode as charging current limiter resistance.Structure design of the present invention is simple,
It is cost-effective, it can realize the automatic inflatable Electricity Functional of bactericidal unit, inoculating tool, without connection, passes through contact with bactericidal unit
Realize sterilizing function, high efficiency and safety during the guarantee use of high degree, meanwhile, bactericidal unit contact inoculation portion
The lower electrode divided can realize self-cleaning function, further improve the sterilization effect of bactericidal unit.
Description of the drawings
A kind of structure diagram of the Quick sterilizing device of Fig. 1-microbial inoculant tool;
Transfer needle structure diagram in Fig. 2-Fig. 1;
The vertical view of top electrode in Fig. 3-Fig. 1;
Control circuit schematic diagram in Fig. 4-Fig. 1.
In figure:1-transfer needle, 11-insulated handle, 12-metal tie rods, 13-wire, 2-support base, 3-
Strut, 4-top electrode, 5-lower electrode, 6-storage capacitor, 7-external power supply, 8-control circuit.
Specific embodiment
The invention will be further described with reference to the accompanying drawings and embodiments.
As shown in drawings, a kind of Quick sterilizing device of microbial inoculant tool, including transfer needle 1 and bactericidal unit, connects
Kind needle 1 includes insulated handle 11, metal tie rods 12 and wire 13, and three parts are sequentially connected from top to bottom, and wire 13 is
Nichrome is made, overall length 50mm, a diameter of 1mm.Bactericidal unit includes support base 2, supporting rod 3, top electrode 4, lower electricity
Pole 5, storage capacitor 6, battery 7 and control circuit 8, support base 2 and supporting rod 3 are insulating materials, and lower electrode 5 is arranged on support base
2 upper surfaces, supporting rod 3 connect top electrode 4 and support base 2, top electrode 4 and 3 movable connection of supporting rod, are located at supporting rod 3
On height can determine that upper/lower electrode 5 is connected by conducting wire according to the height of used wire 13 and metal tie rods 12
Storage capacitor 6 and control circuit 8, during sterilizing, the lower electrode 5 of needle ring part contact of transfer needle 1 or oese, metal bar part connects
Touch top electrode 4.
Lower electrode is the pellet resistance of 3*0.1mm, and the length of 60mm in its punching two ends and passes through screw connection manner respectively
It is fixed on support base 2 and distinguishes extraction electrode wiring in bearing.As T1, T0 contact conductors, connection T1 and T0 ends are gone out
The closing structure that one end is embedded circular arc type is made of the stainless steel tube or copper pipe of diameter 3-6mm for the top electrode of bacterium device.
The anode of storage capacitor 6C1 is electrically connected with T1 terminals, and T1 terminals are connected to one of change-over switch S1 simultaneously
On normally opened contact, the common of change-over switch S1 connects GND ends.The cathode of storage capacitor 6C1 connects the normally closed of change-over switch S1 and touches
Point, under charged state, the cathode of storage capacitor 6C1 is connected to GND ends to carry out charging energy-storing by change-over switch.Anti-interference capacitor
C2 mono- terminates+12V positive terminals, a termination GND negative pole ends.
The anode of battery 7 connects the+12V terminals of bactericidal unit, and 4 feet and 8 feet of 555 time base circuits also connect+12V terminals, field
The D poles of effect pipe VT connect+12V terminals, and the source S of field-effect tube VT meets one end T0 of R1, other end the connecting terminal T1, T1 of R1
As 5 electrical terminal of lower electrode of bactericidal unit, while 6 feet of 555 time base circuits are connect, 2 feet of 555 time base circuits connect electricity
One end of R3 is hindered, the other end of R3 is connected on T1 terminals to transmit T1 terminal level change states.The 2 of 555 time base circuits
Another pin that foot is further connected with microswitch a S2, S2 connects GND ends.Press microswitch S2, the 2 of 555 time base circuits
Foot with input low level, can make 3 feet of 555 time base circuits export high level.It connects and is full of in 7 foot of discharge end of 555 time base circuits
The cathode of indicator light light emitting diode D1, the anode of D1 connect a pin of current-limiting resistance R4, and another pin of R4 is connected to+
On 12V terminals, when storage capacitor 6 is fully charged(About 8V voltages)When, it shines full of indicator light light emitting diode D1, represents energy storage
6 charging complete of capacitance.
The operation principle and using method of the present invention:+ the 12V of control circuit 8 connects the anode of battery 7, and GND connects battery 7
Cathode, 555 time base circuits are as 6 charging control circuit 8 of storage capacitor.
After connecting battery 7,2 feet of 555 time base circuits are because of the presence of storage capacitor 6C1, and input low level, at this point,
The output high level driving high current field-effect tube VT conductings of its 3 foot, the internal triode C poles open circuit of 7 feet output ,+12V voltages pass through
Field-effect tube VT charges to capacitor C1 through R1, and C1 terminal voltages is made to increase.When C1 terminal voltages rise to about 8V, charge
Into, at this point, 6 feet of time base circuit obtain high level input, 555 time base circuit, 3 foot output low level, field-effect tube VT is disconnected,
And it no longer charges to C1.Meanwhile 555 time base circuit 7 feet inside triode saturation conduction, C poles are equivalent to short-circuit shape over the ground
State shines full of indicator light D1, represents that storage capacitor 6 fills with charge, instruction operating personnel can carry out inoculating tool
Disinfecting action.
The lower electrode 5 of bactericidal unit connects port T1 at the anode of storage capacitor 6C1, and top electrode 4 connects the port at GND ends
T2, during sterilizing, operating personnel contact the wire 13 of transfer needle 1 with lower electrode 5,12 metal part of connecting rod of transfer needle 1
It is contacted with the top electrode 4 of bactericidal unit, electric current is formed between top electrode 4, transfer needle 1, lower electrode 5 and storage capacitor 6C1 and is returned
Road, storage capacitor 6C1 electric discharges, the wire 13 of transfer needle 1(Resistance wire)In be able to by larger electric current it is rapid heating up,
So as to which calcination wire 13 achievees the purpose that sterilizing.
After the completion of sterilizing, transfer needle 1 leaves top electrode 4 and lower electrode 5, and the electric loop of formation interrupts, in wire 13
Current vanishes, heating stop.Meanwhile the moment being detached from transfer needle 1 and top electrode 4 and lower electrode 5, the 2 of 555 time base circuits
Foot inputs a low level pulse, and storage capacitor 6C1 carries out boost charge immediately, prepares for sterilizing use next time.
When the lower electrode 5 of bactericidal unit needs automatically cleaning because of pollution, change-over switch S1 is got into automatically cleaning shelves temporarily,
At this point, the normally-closed contact of change-over switch S1 disconnects, storage capacitor 6C1 cathode are hanging, meanwhile, T1 terminals pass through change-over switch S1's
Normally opened contact connects common and realizes electrical connection with GND ends, and 2 feet of 555 time base circuits are connected by resistance R3 and T1 terminals
It connects, 2 feet of 555 time base circuits obtain low level input, and field-effect tube VT is connected in 3 feet output high level, and heater R1 obtains electric
The lower electrode 5 of fever heating, makes lower 5 temperature of electrode reach about 300 DEG C of high temperature and reach lower 5 automatically cleaning purpose of electrode.
Claims (10)
1. a kind of Quick sterilizing device of microbial inoculant tool, it is characterised in that:Including transfer needle(Ring), support base, support
Bar, top electrode, lower electrode, energy-storage travelling wave tube, external power supply and control circuit, transfer needle are followed successively by insulated handle, gold from top to bottom
Belong to connecting rod and wire, support base and supporting rod are insulating materials, and lower electrode is arranged on support base upper surface, supporting rod connection
Top electrode and support base, upper/lower electrode connects energy-storage travelling wave tube by conducting wire and control circuit, extraneous power supply are connect with control circuit
Electric energy is provided;During sterilizing, the transfer needle(Ring)Wire contact under electrode, metal tie rods part contact top electrode, storage
Energy component discharges, after sterilizing, transfer needle(Ring)It is detached from upper/lower electrode, energy-storage travelling wave tube charging.
2. a kind of Quick sterilizing device of microbial inoculant tool as described in claim 1, it is characterised in that:The energy storage member
Part is large capacity farad grade capacitance.
3. a kind of Quick sterilizing device of microbial inoculant tool as described in claim 1, it is characterised in that:The energy storage member
Microswitch is connected at part.
4. a kind of Quick sterilizing device of microbial inoculant tool as described in claim 1, it is characterised in that:The top electrode
The closing structure that one end is embedded circular arc type is made by the stainless steel tube or copper pipe of diameter 3-6mm, top electrode can live with supporting rod
Dynamic connection, top electrode position is adjustable.
5. a kind of Quick sterilizing device of microbial inoculant tool as described in claim 1, it is characterised in that:The lower electrode
For the pellet resistance of 3*0.1mm, length is in 50-60mm.
6. a kind of Quick sterilizing device of microbial inoculant tool as claimed in claim 1 or 2, it is characterised in that:On described
Connection change-over switch or switching relay among electrode tip and energy-storage travelling wave tube.
7. a kind of Quick sterilizing device of microbial inoculant tool as described in claim 1, it is characterised in that:The control electricity
Road is 555 time base circuits.
8. a kind of Quick sterilizing device of microbial inoculant tool as described in claim 1, it is characterised in that:The wire
It is made of platinum, nichrome or Aludirome, overall length is 30 ~ 60mm, a diameter of 0.5 ~ 1mm.
9. a kind of Quick sterilizing device of microbial inoculant tool as described in claim 1 or 8, it is characterised in that:The gold
It is movable connection to belong to silk with metal tie rods.
10. a kind of Quick sterilizing device of microbial inoculant tool as described in claim 1, it is characterised in that:It is described external
Power supply is 12V batteries, 12V Switching Power Supplies or the 12V DC power supply after transformer transformation.
Priority Applications (1)
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CN201810188994.2A CN108165477B (en) | 2018-03-08 | 2018-03-08 | Quick sterilization device of microorganism inoculation instrument |
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CN201810188994.2A CN108165477B (en) | 2018-03-08 | 2018-03-08 | Quick sterilization device of microorganism inoculation instrument |
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CN108165477B CN108165477B (en) | 2023-09-26 |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113366948A (en) * | 2021-05-06 | 2021-09-10 | 张改英 | Device and method for sterilizing weed seeds in farmland at instantaneous high temperature |
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CN2168589Y (en) * | 1993-10-08 | 1994-06-15 | 济南绿宝电子生物技术公司 | Disinfecting and preserving equipment |
CN1833728A (en) * | 2006-04-25 | 2006-09-20 | 贾会生 | Fulgerizing sterilizer of oese (needle) |
JP2008113930A (en) * | 2006-11-07 | 2008-05-22 | Airtech Japan Ltd | Electric loop sterilizer |
CN205031579U (en) * | 2015-09-29 | 2016-02-17 | 河南正清源生物科技有限公司 | Intelligence transfering loop / inoculating needle steriliser |
CN105703659A (en) * | 2016-04-07 | 2016-06-22 | 厦门大学 | High-frequency and high-voltage pulse generator for sterilizing |
CN206273828U (en) * | 2016-07-29 | 2017-06-23 | 北京成大天和生物科技有限公司 | High-temperature electric heating disinfection is inoculated with loop device |
CN107988059A (en) * | 2018-01-22 | 2018-05-04 | 李长寿 | It is a kind of to breed inoculation instrument from the culture of disinfection type microbial bacterial |
CN208104412U (en) * | 2018-03-08 | 2018-11-16 | 湖南水口山有色金属集团有限公司 | A kind of Quick sterilizing device of microbial inoculant tool |
KR20190017472A (en) * | 2017-08-11 | 2019-02-20 | 가톨릭대학교 산학협력단 | self sterilizing inoculation loop |
CN211814449U (en) * | 2020-03-09 | 2020-10-30 | 李志会 | High-efficiency energy-saving electronic inoculation device |
-
2018
- 2018-03-08 CN CN201810188994.2A patent/CN108165477B/en active Active
Patent Citations (10)
Publication number | Priority date | Publication date | Assignee | Title |
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CN2168589Y (en) * | 1993-10-08 | 1994-06-15 | 济南绿宝电子生物技术公司 | Disinfecting and preserving equipment |
CN1833728A (en) * | 2006-04-25 | 2006-09-20 | 贾会生 | Fulgerizing sterilizer of oese (needle) |
JP2008113930A (en) * | 2006-11-07 | 2008-05-22 | Airtech Japan Ltd | Electric loop sterilizer |
CN205031579U (en) * | 2015-09-29 | 2016-02-17 | 河南正清源生物科技有限公司 | Intelligence transfering loop / inoculating needle steriliser |
CN105703659A (en) * | 2016-04-07 | 2016-06-22 | 厦门大学 | High-frequency and high-voltage pulse generator for sterilizing |
CN206273828U (en) * | 2016-07-29 | 2017-06-23 | 北京成大天和生物科技有限公司 | High-temperature electric heating disinfection is inoculated with loop device |
KR20190017472A (en) * | 2017-08-11 | 2019-02-20 | 가톨릭대학교 산학협력단 | self sterilizing inoculation loop |
CN107988059A (en) * | 2018-01-22 | 2018-05-04 | 李长寿 | It is a kind of to breed inoculation instrument from the culture of disinfection type microbial bacterial |
CN208104412U (en) * | 2018-03-08 | 2018-11-16 | 湖南水口山有色金属集团有限公司 | A kind of Quick sterilizing device of microbial inoculant tool |
CN211814449U (en) * | 2020-03-09 | 2020-10-30 | 李志会 | High-efficiency energy-saving electronic inoculation device |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113366948A (en) * | 2021-05-06 | 2021-09-10 | 张改英 | Device and method for sterilizing weed seeds in farmland at instantaneous high temperature |
CN113366948B (en) * | 2021-05-06 | 2022-06-17 | 张改英 | Device and method for sterilizing weed seeds in farmland at instantaneous high temperature |
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