CN108157591A - A kind of preparation method for promoting standing grain flower carp meat nonreactive feed - Google Patents
A kind of preparation method for promoting standing grain flower carp meat nonreactive feed Download PDFInfo
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Abstract
The present invention provides a kind of preparation method for promoting standing grain flower carp meat nonreactive feed, the described method comprises the following steps：Papain and bromelain are digested, and 20~50 DEG C of fermentations, 55 DEG C~65 DEG C puffing, granulation, obtain standing grain flower carp nonreactive feed.The present invention is not under the premise of antibiotic is added, pass through controlled enzymatic hydrolysis condition, the small peptide fragment of absorption specific protein of suitable standing grain flower carp is prepared, ensure the speed of growth of standing grain flower carp, efficiently solve the defects of prior art is easily remained and developed immunity to drugs in standing grain flower carp body, brings hidden danger to the food security of the mankind；The rational material matching of the present invention makes that standing grain flower carp meat is solid, fresh fragrant, full color simultaneously so that the standing grain flower carp meat of raising reaches the level of wild standing grain flower carp, effectively improves the economic benefit of standing grain flower carp cultivation.
Description
Technical field
The present invention relates to aquatic animal feed technical field, particularly a kind of preparation for promoting standing grain flower carp meat nonreactive feed
Method.
Background technology
Standing grain flower carp is ominivore-fish, has cold-resistant, high temperature resistant, lower oxygen concentration resistance grows the characteristics of fast.Fish body crow brown,
The characteristics of most apparent compared with common carp is its abdomen purplish, and spire squama, meat is extremely delicate, has main thorn without spinelet,
Build is between carp crucian carp.Growing way speed is faster than crucian, is slightly slower than carp.Current year seed to the end of the year up to 1 jin or so, cultivation side
The same carp of method, crucian.Various breeding way (pond, flowing water, net cage, river and paddy field aquaculture) are adapted to, but with paddy field aquaculture
For certified products.
Standing grain flower carp living accommodation is strong, and yield is not high under original paddy field aquaculture pattern, and cultivation situation can preferably reach
About 50 kgs/acre.Develop pond culture pattern at present, pond culture have pond utilization rate height, small investment, high efficiency spy
Point.Standing grain flower carp has stringent listing specification, general 30~50g/ tails, and the fish price more than this specification will reduce very much.Cause
This is raised from after fingerling dispensing begins to fishing listing for 40 days or so, later every the primary listing of fishing in 7~10 days, until all
Listing.Other than common pond culture pattern, the one of the paddy field aquaculture Hehua fish of hazard prevention Quanzhou County development in recent years
Kind new model, is called field pool coalescence mode.In such a mode, make 10%~20% area in rice field into fish pond, and fish pond with
Rice field is mutually communicated, and then increases breeding density, and yield can be increased to 1.5~2 times of original yield in such a mode.
It needs to feed perfect compound feed in the case where standing grain spends carp pond culture pattern, in some paddy field aquacultures and field pool perforation mould
Also it is required for feeding perfect compound feed under formula as supplement bait.However dedicated for standing grain flower carp cultivation in existing market
Feed type it is rare, general raiser is replaced using common freshwater fish feed, and feed coefficient is higher, productivity effect compared with
It is low.
Prior art discloses some for the feed of standing grain flower carp, such as a kind of cooperations of standing grain flower carp juvenile fish of CN104323031A
A kind of natural feeds of standing grain flower carp of feed, CN104872444A a kind of mixed feed, the CN105166445A of standing grain flower carp.These are raised
Material can spend carp provide growth required nutritional ingredient for standing grain, but these feeds contain that antibiotic is more, influence the meat that standing grain spends carp.
Invention content
The present invention provides a kind of preparation method for promoting standing grain flower carp meat nonreactive feed, in the premise for not adding antibiotic
Under, by controlled enzymatic hydrolysis condition, the small peptide fragment of absorption specific protein of suitable standing grain flower carp is prepared, while pass through fermentation condition
Control nonreactive feed is made, efficiently solve the prior art and easily remain and develop immunity to drugs, to the mankind in standing grain flower carp body
Food security the defects of bringing hidden danger.
Technical solution is realized under the purpose of the present invention：
Step 1, enzymolysis：Tilapia mossambica processing byproduct is subjected to boiling, papain and bromelain are added after cooling
Enzyme is digested, 42~55 DEG C of hydrolysis temperature, pH value 7.5~9,3~6h of enzymolysis time；Enzymolysis Rofe Fish protein is obtained after filtering
Peptide liquid, by being concentrated and dried, generation enzymolysis Tilapia mossambica protein small peptide powder；
Step 2, fermentation：Following substance is taken according to parts by weight：The Tilapia mossambica protein small peptide powder 25~45 that step 1 obtains
Part, 15~25 parts of dregs of beans, 15~20 parts of wheat, 10~15 parts of rapeseed meal, 5~15 parts of rice bran, 1~5 part of brewer's yeast, cellulase
3~5 parts, 1~3 part of humic acid, add in 4~6 times of weight waters and mix it is quenched, be uniformly mixed be placed in fermenting cellar
It ferments at 20~50 DEG C, fermentation time is 15~20 days；
Step 3, it is puffing：The gains of step 2 after fermentation are dried, be sufficiently mixed it is rear puffing, it is puffing
Temperature is 55 DEG C~65 DEG C, and puffing time is 1.5~3 hours, is 80%~95% by the aridity of bulking meterial；
Step 4, granulation：Material after will be puffing, which is transferred in granulator, is produced into precipitation hard particles feed, and packaging obtains
Obtain standing grain flower carp nonreactive feed.
Preferably, the condition digested in the step 1 be temperature 50 C, pH value 8, enzymolysis time 4.5h；
Preferably, to be concentrated in vacuo, vacuum degree is 0.04MPa~0.08MPa for concentration in the step 1；
Preferably, the mass fraction of each component is in the step 2：30 parts of protein peptide powder, 20 parts of dregs of beans, 18 parts of wheat,
12 parts of rapeseed meal, 10 parts of rice bran, 3 parts of brewer's yeast, 4 parts of cellulase, 2 parts of humic acid；
Preferably, the condition fermented in the step 2 is：It ferments at 30 DEG C, fermentation time is 18 days.
Preferably, condition puffing in the step 3 is：Temperature 60 C, puffing time is 2 hours, by bulking meterial
Aridity is 87%.
Advantages of the present invention：
1. the present invention efficiently solves the prior art and easily remains and develop immunity to drugs, the food to the mankind in standing grain flower carp body
The defects of product safety belt carrys out hidden danger.
2. under the premise of antibiotic is not added, by controlled enzymatic hydrolysis condition, relative molecular mass is prepared in the present invention
The Tilapia mossambica protein small peptide of ranging from 6.5k~9.5k, the specific fragment are particularly suitable for the absorption of standing grain flower carp.
3. material matching of the invention rational makes, standing grain flower carp meat is solid, fresh fragrant, full color so that the standing grain flower carp of raising
Meat reaches the level of wild standing grain flower carp, effectively improves the economic benefit of standing grain flower carp cultivation.
Specific embodiment
With reference to specific embodiment, make further details of elaboration to the present invention；Following embodiment is used
In illustrating the present invention, but be not limited to the scope of the present invention.
Embodiment 1
Step 1, enzymolysis：Tilapia mossambica processing byproduct is subjected to boiling, 1 part of papain and 1 part of spinach are added after cooling
Trailing plants protease is digested, 42 DEG C of hydrolysis temperature, pH value 7.5, enzymolysis time 3h；Enzymolysis Rofe fish protein peptide is obtained after filtering
Liquid, by the vacuum concentration drying that vacuum degree is 0.04MPa, generation enzymolysis Tilapia mossambica protein small peptide powder；
Step 2, fermentation：According to parts by weight take 15 parts of 25 parts of Tilapia mossambica protein small peptide powder, dregs of beans that step 1 obtains,
15 parts of wheat, 10 parts of rapeseed meal, 5 parts of rice bran, 1 part of brewer's yeast, 3 parts of cellulase, 1 part of humic acid add in 4 times of weight waters and go forward side by side
Row mixing is quenched, and being placed in fermenting cellar for being uniformly mixed is fermented at 20 DEG C, and fermentation time is 15 days；
Step 3, it is puffing：The gains of step 2 after fermentation are dried, be sufficiently mixed it is rear puffing, it is puffing
Temperature is 55 DEG C, and puffing time is 1.5 hours, is 80% by the aridity of bulking meterial；
Step 4, granulation：Material after will be puffing, which is transferred in granulator, is produced into precipitation hard particles feed, and packaging obtains
Obtain standing grain flower carp nonreactive feed.
Embodiment 2
Step 1, enzymolysis：Tilapia mossambica processing byproduct is subjected to boiling, 8 parts of papains and 8 parts of spinach are added after cooling
Trailing plants protease is digested, 55 DEG C of hydrolysis temperature, pH value 9, enzymolysis time 6h；Enzymolysis Rofe fish protein peptide liquid is obtained after filtering,
By the vacuum concentration drying that vacuum degree is 0.08MPa, generation enzymolysis Tilapia mossambica protein small peptide powder；
Step 2, fermentation：According to parts by weight take 25 parts of 45 parts of Tilapia mossambica protein small peptide powder, dregs of beans that step 1 obtains,
20 parts of wheat, 15 parts of rapeseed meal, 15 parts of rice bran, 5 parts of brewer's yeast, 5 parts of cellulase, 3 parts of humic acid add in 6 times of weight waters simultaneously
Mix quenched, what is be uniformly mixed is placed in fermenting cellar and ferments at 50 DEG C, and fermentation time is 20 days；
Step 3, it is puffing：The gains of step 2 after fermentation are dried, be sufficiently mixed it is rear puffing, it is puffing
Temperature is 65 DEG C, and puffing time is 3 hours, is 95% by the aridity of bulking meterial；
Step 4, granulation：Material after will be puffing, which is transferred in granulator, is produced into precipitation hard particles feed, and packaging obtains
Obtain standing grain flower carp nonreactive feed.
Embodiment 3
Step 1, enzymolysis：Tilapia mossambica processing byproduct is subjected to boiling, 3 parts of papains and 3 parts of spinach are added after cooling
Trailing plants protease is digested, 50 DEG C of hydrolysis temperature, pH value 8, enzymolysis time 4.5h；Enzymolysis Rofe fish protein peptide is obtained after filtering
Liquid, by the vacuum concentration drying that vacuum degree is 0.06MPa, generation enzymolysis Tilapia mossambica protein small peptide powder；
Step 2, fermentation：According to parts by weight take 20 parts of 30 parts of Tilapia mossambica protein small peptide powder, dregs of beans that step 1 obtains,
18 parts of wheat, 12 parts of rapeseed meal, 10 parts of rice bran, 3 parts of brewer's yeast, 4 parts of cellulase, 2 parts of humic acid add in 5 times of weight waters simultaneously
Mix quenched, what is be uniformly mixed is placed in fermenting cellar and ferments at 30 DEG C, and fermentation time is 18 days；
Step 3, it is puffing：The gains of step 2 after fermentation are dried, are sufficiently mixed rear puffing, puffing temperature
It is 60 DEG C to spend, and puffing time is 2 hours, is 87% by the aridity of bulking meterial；
Step 4, granulation：Material after will be puffing, which is transferred in granulator, is produced into precipitation hard particles feed, and packaging obtains
Obtain standing grain flower carp nonreactive feed.
Embodiment 4
Step 1, enzymolysis：Tilapia mossambica processing byproduct is subjected to boiling, 4 parts of papains and 5 parts of spinach are added after cooling
Trailing plants protease is digested, 50 DEG C of hydrolysis temperature, pH value 8.5, enzymolysis time 5h；Enzymolysis Rofe fish protein peptide is obtained after filtering
Liquid, by the vacuum concentration drying that vacuum degree is 0.07MPa, generation enzymolysis Tilapia mossambica protein small peptide powder；
Step 2, fermentation：According to parts by weight take 22 parts of 40 parts of Tilapia mossambica protein small peptide powder, dregs of beans that step 1 obtains,
18 parts of wheat, 14 parts of rapeseed meal, 13 parts of rice bran, 4 parts of brewer's yeast, 4 parts of cellulase, 2 parts of humic acid add in 6 times of weight waters simultaneously
Mix quenched, what is be uniformly mixed is placed in fermenting cellar and ferments at 45 DEG C, and fermentation time is 19 days；
Step 3, it is puffing：The gains of step 2 after fermentation are dried, be sufficiently mixed it is rear puffing, it is puffing
Temperature is 62 DEG C, and puffing time is 2.5 hours, is 93% by the aridity of bulking meterial；
Step 4, granulation：Material after will be puffing, which is transferred in granulator, is produced into precipitation hard particles feed, and packaging obtains
Obtain standing grain flower carp nonreactive feed.
Embodiment 5
Step 1, enzymolysis：Tilapia mossambica processing byproduct is subjected to boiling, 5 parts of papains and 6 parts of spinach are added after cooling
Trailing plants protease is digested, 45 DEG C of hydrolysis temperature, pH value 8.7, enzymolysis time 3.5h；Enzymolysis Rofe fish protein peptide is obtained after filtering
Liquid, by the vacuum concentration drying that vacuum degree is 0.05MPa, generation enzymolysis Tilapia mossambica protein small peptide powder；
Step 2, fermentation：According to parts by weight take 18 parts of 30 parts of Tilapia mossambica protein small peptide powder, dregs of beans that step 1 obtains,
18 parts of wheat, 11 parts of rapeseed meal, 7 parts of rice bran, 2 parts of brewer's yeast, 3 parts of cellulase, 1 part of humic acid add in 4 times of weight waters and go forward side by side
Row mixing is quenched, and being placed in fermenting cellar for being uniformly mixed is fermented at 28 DEG C, and fermentation time is 16 days；
Step 3, it is puffing：The gains of step 2 after fermentation are dried, be sufficiently mixed it is rear puffing, it is puffing
Temperature is 58 DEG C, and puffing time is 1.8 hours, is 83% by the aridity of bulking meterial；
Step 4, granulation：Material after will be puffing, which is transferred in granulator, is produced into precipitation hard particles feed, and packaging obtains
Obtain standing grain flower carp nonreactive feed.
Embodiment 6
Step 1, enzymolysis：Tilapia mossambica processing byproduct is subjected to boiling, 7 parts of papains and 7 parts of spinach are added after cooling
Trailing plants protease is digested, 50 DEG C of hydrolysis temperature, pH value 7.8, enzymolysis time 4h；Enzymolysis Rofe fish protein peptide is obtained after filtering
Liquid, by the vacuum concentration drying that vacuum degree is 0.07MPa, generation enzymolysis Tilapia mossambica protein small peptide powder；
Step 2, fermentation：According to parts by weight take 25 parts of 45 parts of Tilapia mossambica protein small peptide powder, dregs of beans that step 1 obtains,
15 parts of wheat, 10 parts of rapeseed meal, 15 parts of rice bran, 5 parts of brewer's yeast, 4 parts of cellulase, 2 parts of humic acid add in 5 times of weight waters simultaneously
Mix quenched, what is be uniformly mixed is placed in fermenting cellar and ferments at 35 DEG C, and fermentation time is 18 days；
Step 3, it is puffing：The gains of step 2 after fermentation are dried, be sufficiently mixed it is rear puffing, it is puffing
Temperature is 62 DEG C, and puffing time is 2 hours, is 95% by the aridity of bulking meterial；
Step 4, granulation：Material after will be puffing, which is transferred in granulator, is produced into precipitation hard particles feed, and packaging obtains
Obtain standing grain flower carp nonreactive feed.
Comparative example 1~4 is used to evaluate influence of the feed to absorption made from papain and bromelain
Comparative example 1：Mass fraction is used to replace papain and pineapple in embodiment 3 for 3 parts of papain
Protease, other steps are the same as embodiment 3.
Concrete operations are as follows：
Step 1, enzymolysis：Tilapia mossambica processing byproduct is subjected to boiling, 3 parts of papain is added after cooling and is carried out
Enzymolysis, 50 DEG C of hydrolysis temperature, pH value 8, enzymolysis time 4.5h；Enzymolysis Rofe fish protein peptide liquid is obtained after filtering, by vacuum degree
For the vacuum concentration drying of 0.06MPa, generation enzymolysis Tilapia mossambica protein small peptide powder；
Step 2, fermentation：According to parts by weight take 20 parts of 30 parts of Tilapia mossambica protein small peptide powder, dregs of beans that step 1 obtains,
18 parts of wheat, 12 parts of rapeseed meal, 10 parts of rice bran, 3 parts of brewer's yeast, 4 parts of cellulase, 2 parts of humic acid add in 5 times of weight waters simultaneously
Mix quenched, what is be uniformly mixed is placed in fermenting cellar and ferments at 30 DEG C, and fermentation time is 18 days；
Step 3, it is puffing：The gains of step 2 after fermentation are dried, are sufficiently mixed rear puffing, puffing temperature
It is 60 DEG C to spend, and puffing time is 2 hours, is 87% by the aridity of bulking meterial；
Step 4, granulation：Material after will be puffing, which is transferred in granulator, is produced into precipitation hard particles feed, and packaging obtains
Obtain standing grain flower carp nonreactive feed.
Comparative example 2：Mass fraction is used to replace papain and pineapple in embodiment 3 for 3 parts of bromelain
Protease, other steps are the same as embodiment 3.
Concrete operations are as follows：
Step 1, enzymolysis：Tilapia mossambica processing byproduct is subjected to boiling, 3 parts of bromelain is added after cooling and is carried out
Enzymolysis, 50 DEG C of hydrolysis temperature, pH value 8, enzymolysis time 4.5h；Enzymolysis Rofe fish protein peptide liquid is obtained after filtering, by vacuum degree
For the vacuum concentration drying of 0.06MPa, generation enzymolysis Tilapia mossambica protein small peptide powder；
Step 2, fermentation：According to parts by weight take 20 parts of 30 parts of Tilapia mossambica protein small peptide powder, dregs of beans that step 1 obtains,
18 parts of wheat, 12 parts of rapeseed meal, 10 parts of rice bran, 3 parts of brewer's yeast, 4 parts of cellulase, 2 parts of humic acid add in 5 times of weight waters simultaneously
Mix quenched, what is be uniformly mixed is placed in fermenting cellar and ferments at 30 DEG C, and fermentation time is 18 days；
Step 3, it is puffing：The gains of step 2 after fermentation are dried, are sufficiently mixed rear puffing, puffing temperature
It is 60 DEG C to spend, and puffing time is 2 hours, is 87% by the aridity of bulking meterial；
Step 4, granulation：Material after will be puffing, which is transferred in granulator, is produced into precipitation hard particles feed, and packaging obtains
Obtain standing grain flower carp nonreactive feed.
Comparative example 3：Mass fraction is used to replace papain and pineapple egg in embodiment 3 for 3 parts of trypsase
White enzyme, other steps are the same as embodiment 3.
Concrete operations are as follows：
Step 1, enzymolysis：Tilapia mossambica processing byproduct is subjected to boiling, 3 parts of trypsase is added after cooling and carries out enzyme
Solution, 50 DEG C of hydrolysis temperature, pH value 8, enzymolysis time 4.5h；Enzymolysis Rofe fish protein peptide liquid is obtained after filtering, is by vacuum degree
The vacuum concentration drying of 0.06MPa, generation enzymolysis Tilapia mossambica protein small peptide powder；
Step 2, fermentation：According to parts by weight take 20 parts of 30 parts of Tilapia mossambica protein small peptide powder, dregs of beans that step 1 obtains,
18 parts of wheat, 12 parts of rapeseed meal, 10 parts of rice bran, 3 parts of brewer's yeast, 4 parts of cellulase, 2 parts of humic acid add in 5 times of weight waters simultaneously
Mix quenched, what is be uniformly mixed is placed in fermenting cellar and ferments at 30 DEG C, and fermentation time is 18 days；
Step 3, it is puffing：The gains of step 2 after fermentation are dried, are sufficiently mixed rear puffing, puffing temperature
It is 60 DEG C to spend, and puffing time is 2 hours, is 87% by the aridity of bulking meterial；
Step 4, granulation：Material after will be puffing, which is transferred in granulator, is produced into precipitation hard particles feed, and packaging obtains
Obtain standing grain flower carp nonreactive feed.
Comparative example 4：Mass fraction is used to replace papain and pineapple in embodiment 3 for 3 parts of alkali protease
Protease, other steps are the same as embodiment 3.
Concrete operations are as follows：
Step 1, enzymolysis：Tilapia mossambica processing byproduct is subjected to boiling, 3 parts of papain is added after cooling and is carried out
Enzymolysis, 50 DEG C of hydrolysis temperature, pH value 8, enzymolysis time 4.5h；Enzymolysis Rofe fish protein peptide liquid is obtained after filtering, by vacuum degree
For the vacuum concentration drying of 0.06MPa, generation enzymolysis Tilapia mossambica protein small peptide powder；
Step 2, fermentation：According to parts by weight take 20 parts of 30 parts of Tilapia mossambica protein small peptide powder, dregs of beans that step 1 obtains,
18 parts of wheat, 12 parts of rapeseed meal, 10 parts of rice bran, 3 parts of brewer's yeast, 4 parts of cellulase, 2 parts of humic acid add in 5 times of weight waters simultaneously
Mix quenched, what is be uniformly mixed is placed in fermenting cellar and ferments at 30 DEG C, and fermentation time is 18 days；
Step 3, it is puffing：The gains of step 2 after fermentation are dried, are sufficiently mixed rear puffing, puffing temperature
It is 60 DEG C to spend, and puffing time is 2 hours, is 87% by the aridity of bulking meterial；
Step 4, granulation：Material after will be puffing, which is transferred in granulator, is produced into precipitation hard particles feed, and packaging obtains
Obtain standing grain flower carp nonreactive feed.
Example 1~6 and 1~4 gained feed of comparative example carry out the administering transgenic of standing grain flower carp, and concrete operations are as follows：
After culture experiment, fish body starvation is weighed for 24 hours, is taken out 6 tail fishes at random in every repeating groups and is made amino acid and pigment
Analysis etc..With Hitachi's L-8900 amino-acid analyzers amino acid (acid-hydrolysis method) is measured according to GB/T 5009.124-2003
The measure of astaxanthin：
Ridge more than every tail fish both sides side line takes the meat piece of 2 0.4g or so to measure from pars muscularis point, by mass body
Product is than adding in chloroform-ethanol (1：1) real 10 times of dilution agent is mixed, shreds homogenate to suspended liquid, the centrifuge tube for being transferred to 7mL is put
8000r/min centrifugation 10min are taken out in 50 DEG C of water-baths, after 45min, supernatant is taken to measure absorbance under 487nm wavelength
Value.Now adopt existing survey.Concrete outcome is shown in Table 1.
1 full fish of table and nutritive composition in muscle
As seen from the above table, the standing grain flower carp of forage feed of the present invention, flesh of fish consolidation, meat fiber are compared with the standing grain that normal diet is fed
Flower carp is thin, and astaxanthin concentration is apparently higher than the standing grain flower carp of normal diet nursing, and the level of carp, while flavor ammonia are spent close to wild standing grain
Base acid (asparagine, glutamic acid, alanine, glycine) content is also apparently higher than normal diet nursing group, is spent close to wild standing grain
The content of carp flavor amino acid illustrates that feed of the present invention significantly improves the quality of standing grain flower carp meat.
Relatively for the wild standing grain flower carp of natural environment, the crude protein of the same race of raising standing grain flower carp is less, fragrance is light, flesh of fish face
Color is poor, palatability is not good enough.Wild standing grain flower carp is orange red yellowish pink dependent on carotenoid pigment deposition in feed, and cultivates standing grain flower
Carp is general lack of trace element and carotenoid.Flavor component majority in animal organism is C3H5- S (O)-group, standing grain flower carp
Mixed bait in relatively lack this ingredient, therefore the delicate flavour with sweet and sour flavor cultivated is thin.Present invention accomplishes consumer demands, are not having
Under the premise of antibiotic, while increase the fragrance of the flesh of fish.
Carp is spent as experimental subjects using postlarva phase standing grain, the culture-cycle is 6 weeks.Experiment carries out in identical waters, every group of fry 50
Tail is raised in 0.5m3Water body in, feed daily 2 times, feed daily rate be fish body weight 3%-5%.It is set three times per embodiment
It repeats, experiment terminates, detection growth performance, feed conversion degree, as a result such as table 2：
2 growth performance of table, efficiency of feed utilization index
It is apparent that from table 2：Compared with comparative example, the present invention is by being prepared containing relative molecular mass range
The feed of Tilapia mossambica protein small peptide for 6.5k~9.5k is in standing grain flower carp body weight gains, specific growth rate, protein efficiency ratio three
The significant effect of a aspect, is superior to comparative example, improves standing grain flower carp feed absorptivity.
Although above having used general explanation, specific embodiment and experiment, the present invention is made to retouch in detail
It states, but on the basis of the present invention, it can be made some modifications or improvements, this is apparent to those skilled in the art
's.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to claimed
Range.
Claims (6)
1. a kind of preparation method for promoting standing grain flower carp meat nonreactive feed, which is characterized in that include the following steps：
Step 1, enzymolysis：Tilapia mossambica processing byproduct is subjected to boiling, added after cooling papain and bromelain into
Row enzymolysis, 42~55 DEG C of hydrolysis temperature, pH value 7.5~9,3~6h of enzymolysis time；Enzymolysis Rofe fish protein peptide is obtained after filtering
Liquid, by being concentrated and dried, generation enzymolysis Tilapia mossambica protein small peptide powder；
Step 2, fermentation：Following substance is taken according to parts by weight：25~45 parts of Tilapia mossambica protein small peptide powder that step 1 obtains,
15~25 parts of dregs of beans, 15~20 parts of wheat, 10~15 parts of rapeseed meal, 5~15 parts of rice bran, 1~5 part of brewer's yeast, cellulase 3~
5 parts, 1~3 part of humic acid, add in 4~6 times of weight waters and mix it is quenched, be uniformly mixed be placed in fermenting cellar 20
It ferments at~50 DEG C, fermentation time is 15~20 days；
Step 3, it is puffing：The gains of step 2 after fermentation are dried, are sufficiently mixed rear puffing, puffing temperature
It it is 55 DEG C~65 DEG C, puffing time is 1.5~3 hours, is 80%~95% by the aridity of bulking meterial；
Step 4, granulation：Material after will be puffing, which is transferred in granulator, is produced into precipitation hard particles feed, and packaging obtains standing grain
Flower carp nonreactive feed.
A kind of 2. preparation method for promoting standing grain flower carp meat nonreactive feed as described in claim 1, which is characterized in that the step
The condition digested in rapid one be temperature 50 C, pH value 8, enzymolysis time 4.5h.
A kind of 3. preparation method for promoting standing grain flower carp meat nonreactive feed as described in claim 1, which is characterized in that the step
To be concentrated in vacuo, vacuum degree is 0.04MPa~0.08MPa for concentration in rapid one.
A kind of 4. preparation method for promoting standing grain flower carp meat nonreactive feed as described in claim 1, which is characterized in that the step
The mass fraction of each component is in rapid two：30 parts of protein peptide powder, 20 parts of dregs of beans, 18 parts of wheat, 12 parts of rapeseed meal, 10 parts of rice bran, beer
3 parts of brewer yeast, 4 parts of cellulase, 2 parts of humic acid.
A kind of 5. preparation method for promoting standing grain flower carp meat nonreactive feed as described in claim 1, which is characterized in that the step
The condition fermented in rapid two is：It ferments at 30 DEG C, fermentation time is 18 days.
A kind of 6. preparation method for promoting standing grain flower carp meat nonreactive feed as described in claim 1, which is characterized in that the step
Puffing condition is in rapid three：Temperature 60 C, puffing time are 2 hours, are 87% by the aridity of bulking meterial.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110353089A (en) * | 2019-08-20 | 2019-10-22 | 昆明通威饲料有限公司 | Meat quality of java tilapia changes special compound feed and preparation method |
| CN110839757A (en) * | 2019-12-04 | 2020-02-28 | 北京市水产技术推广站 | Goldfish fry starter feed and preparation method thereof |
| CN113100356A (en) * | 2021-05-26 | 2021-07-13 | 广西大学 | Feed formula for young procypris merus |
-
2018
- 2018-03-12 CN CN201810201258.6A patent/CN108157591A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110353089A (en) * | 2019-08-20 | 2019-10-22 | 昆明通威饲料有限公司 | Meat quality of java tilapia changes special compound feed and preparation method |
| CN110839757A (en) * | 2019-12-04 | 2020-02-28 | 北京市水产技术推广站 | Goldfish fry starter feed and preparation method thereof |
| CN113100356A (en) * | 2021-05-26 | 2021-07-13 | 广西大学 | Feed formula for young procypris merus |
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