CN108129204A - A kind of nitrogen-phosphorus synergist of the hydrolyzate containing earthworm and its preparation method and application - Google Patents
A kind of nitrogen-phosphorus synergist of the hydrolyzate containing earthworm and its preparation method and application Download PDFInfo
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- CN108129204A CN108129204A CN201810069227.XA CN201810069227A CN108129204A CN 108129204 A CN108129204 A CN 108129204A CN 201810069227 A CN201810069227 A CN 201810069227A CN 108129204 A CN108129204 A CN 108129204A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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Abstract
The present invention provides a kind of nitrogen-phosphorus synergist of the hydrolyzate containing earthworm and its preparation method and application, the nitrogen-phosphorus synergist of present invention hydrolyzate containing earthworm, it counts in parts by weight, including 15~20 parts of earthworm hydrolyzate, 4~5 parts of phytase, 15~20 parts of citric acid, 20~50 parts of potassium fulvate, 20~25 parts of poly-aspartic-acid.Liquid dosage form, pulvis or granule can be made in the nitrogen-phosphorus synergist as needed.Nitrogen-phosphorus synergist of the present invention uses to have in agricultural production and improves nitrogenous-phosphatic manure fertilizer efficiency, reduces nitrogenous-phosphatic manure amount of application, reduces the effect of nitrogen phosphorus pollution of area source.Show that using nitrogen-phosphorus synergist of the present invention facilities vegetable yield, the use for reducing nitrogenous-phosphatic manure can be improved by facilities vegetable planting experiment, improve the content of rapid available phosphorus and available nitrogen in soil.Soil quality can also be improved, inhibit soil-borne disease.
Description
Technical field
The invention belongs to agricultural technology fields, and in particular to a kind of nitrogen-phosphorus synergist of hydrolyzate containing earthworm and its preparation side
Method and application.
Background technology
In order to pursue the high yield of the crops such as grain, vegetables, peasant often largely applies the fertilizer such as nitrogen, phosphorus, therefore farmland
Soil Nitrogen, phosphorus input amount are far longer than crop demand.If things go on like this, the nutrient content in soil is caused constantly to be accumulated, crop
It is serious to the reduction of nitrogen P assimilation and utilization rate, nitrogen and phosphorus loss, not only nutrient is caused to waste, return soil, underground water and agricultural ecological
Environment etc. brings serious pollution risk.In order to reduce nitrogen and phosphorus pollution, the efficient utilization of nutrient is realized, except reducing nitrogen from source
Outside phosphorus precipitation, it is often more important that improve nitrogen phosphorus plant recovery of nutrient.The prior art is generally from enhancing nitrogen phosphorus activity, improvement soil
Environment promotes being absorbed and utilized for nitrogen phosphorus nutrient.
Earthworm is the larger invertebrate of the biomass of generally existing in soil.As important in terrestrial ecosystems
Ecological factor, earthworm can make loosing soil, improvement ecological environment of soil, build up fertility, and promote agricultural volume increase, maintain soil life
Irreplaceable role is played in state system function.The main function mechanism of Soil of Earthworms environment include physical perturbation effect,
Chemical substance biocatalysis and its with the comprehensive function of microorganism etc..Outside the perturbation action for removing earthworm, main also earthworm
Biochemical in vermis liquid plays an important role.By in earthworm hydrolyzate that earthworm hydrolyzes to form containing a large amount of beneficial to changing
Good soil, the active material improved the ecological environment, promote plant growth etc., such as content polyphenol, plant hormone, amino acid, can
To inhibit soil-borne disease insect pest, promote plant growth, improvement soil quality etc..
The application in agricultural production at present of earthworm hydrolyzate is embodied in and plant growth, volume increase, disease-resistant, raising is promoted to make
Article matter and improvement soil environment etc..There has been no ground using earthworm hydrolyzate to improve the correlation of nitrogen phosphorus plant recovery of nutrient
Study carefully report.
Invention content
The present invention in view of the above shortcomings of the prior art, according to the characteristics of earthworm hydrolyzate, provides a kind of containing earthworm hydrolysis
Nitrogen-phosphorus synergist of liquid and its preparation method and application.
For achieving the above object, the technical solution adopted by the present invention is as follows：A kind of nitrogen phosphorus of hydrolyzate containing earthworm increases
Agent is imitated, is counted in parts by weight, including 15~20 parts of earthworm hydrolyzate, 4~5 parts of phytase, 15~20 parts of citric acid, potassium fulvate
20~50 parts, 20~25 parts of poly-aspartic-acid.
The nitrogen-phosphorus synergist is liquid dosage form, pulvis or granule.
Preferably, the nitrogen-phosphorus synergist of the hydrolyzate containing earthworm, is counted in parts by weight, including 18 parts of earthworm hydrolyzate,
5 parts of phytase, 17 parts of citric acid, 40 parts of potassium fulvate, 23 parts of poly-aspartic-acid, 20~30 parts of water.
The present invention also provides the preparation method of the nitrogen-phosphorus synergist of the hydrolyzate containing earthworm, step is：
(1) preparation of earthworm hydrolyzate：Fresh earthworm is smashed to pieces after cleaning and is homogenized, and adds in water and adjusts pH to 6~8,
Complex enzyme is added in, carries out digesting up to earthworm hydrolyzate under the conditions of insulated and stirred.
(2) preparation of nitrogen-phosphorus synergist, by earthworm hydrolyzate, phytase, citric acid, potassium fulvate, poly-aspartic-acid,
Water is stirred nitrogen-phosphorus synergists of the 15~20min to get the hydrolyzate containing earthworm.
The complex enzyme is subtilopeptidase A and bromelain according to 1:2~3 ratios mix.
Specifically, the step (1) takes fresh earthworm 100-200g, with sterile water wash 3~5 times, earthworm that will be clean
It is placed in high-speed tissue mashing machine, 25~30min is smashed to pieces with the rotating speed of 4000~5000r/min, is homogenized, by homogenate and water
In mass ratio 1:2~1:4 are added in beaker, adjust pH to 6~8 with the dilute hydrochloric acid of a concentration of 0.02mol/L, add 300
~500mg complex enzymes, after being stirred 3~5min, will beaker move into heat-collecting magnetic stirring device in, adjust blender temperature to
40~45 DEG C, speed of agitator to 200~230r/min, 4~5h of enzyme digestion reaction, enzymolysis terminates, enzymolysis liquid is put into centrifuge,
20~25min is centrifuged with the rotating speed of 3500~4500r/min, takes upper liquid, i.e. earthworm hydrolyzate, Cord blood is spare.
Nitrogen-phosphorus synergist of the present invention can also be prepared into pulvis, granule.By earthworm hydrolyzate be concentrated and dried into pulvis with
Other components mixing is pressed further by being prepared into particle.
Moreover, nitrogen-phosphorus synergist of the present invention can also be used to prepare multiple-effect compound nitrogen phosphate fertilizer together with nitrogen, phosphorus component.
Nitrogenous-phosphatic manure fertilizer efficiency is improved in agricultural production, reduce the dirt of nitrogen phosphorus face source the present invention also provides the nitrogen-phosphorus synergist
Application in dye.Specifically, application of the nitrogen-phosphorus synergist of the present invention in facilities vegetable, amount of application are：20~30kg/ mus.
The present inventor shows that using nitrogen-phosphorus synergist of the present invention facilities vegetable can be improved by facilities vegetable planting experiment
Yield, the use for reducing nitrogenous-phosphatic manure improve the content of rapid available phosphorus and available nitrogen in soil.
Earthworm hydrolyzate of the present invention：Contain polyphenol, plant hormone, amino acid and the unknown various active object of other compositions
Matter can not only inhibit soil-borne disease insect pest, promote plant growth, improve soil quality, it is often more important that can also improve fertilizer
Utilization rate.Polyphenols：Stable metallo-chelate is formed with complexing of metal ion, the natural reparation to contaminated soil environment
With positive effect, being absorbed and utilized for trace element can be effectively facilitated, at the same adverse environmental factor residing for plant is played protection and
Adjustment effect improves the ability that plant adapts to external environment.The effect of polyphenols can significantly improve stress resistance of plant, subtract
Few pest and disease damage so as to reach raising crop yield, promotes the effect that largely absorbs of the crop to nitrogen phosphorus nutrient.Plant hormone：Earthworm
The plant hormone auxin (IAA) that contains in earthworm hydrolyzate, gibberellin (GA3) etc., generation development, plant to crop adventitious root
Cauline leaf elongation has good facilitation effect, promotes plant growth, improves crop and nitrogen phosphorus is absorbed and utilized.Amino acid：Earthworm sheet
Body protein content is very high, accounts for the 56%~66% of dry weight, the present invention carries out the earthworm water of enzymolysis acquisition by using complex enzyme
It is even more containing abundant amino acid and a small amount of small peptide to solve in liquid, and wherein amino acid accounts for 85%, and small peptide and small protein account for
14%~15%.Amino acid is promoting plant growth, and effect is remarkably promoted in raising crop yield, enhancing nitrogen phosphorus nutrient.
Antibacterial substance：There are a variety of antimicrobial components in earthworm body, such as antibacterial peptide material, can not only provide for plant and quickly and effectively support
Point, moreover it is possible to inhibit the growth of germ, play a significant role in terms of soil-borne disease insect pest is prevented, cover crop from virus infraction,
Ensure that crop normally produces, improve crop yield, promote a large amount of absorptions to nitrogen phosphorus nutrient.
Detailed description are as follows for the characteristics of nitrogen-phosphorus synergist each component of the present invention and mutual synergistic effect：
Phytase：Organic phosphate is an important supply source of phosphorus element needed for plant growth, and it is organic to be often divided into activity
Phosphorus, medium activity organophosphor, middle stability organophosphor and high stability organophosphor.Wherein activity and medium activity organophosphor are Yi Beizhi
The component that object absorbs.Source of phytase can promote soil stability organophosphor to be converted to active organic phosphorus or even to Phos
Conversion, so as to improve the validity of Organic phosphate element, as phosphorus synergist, phytase can effectively adjust phosphorus element activity.
Citric acid：On the one hand citric acid in the present invention promotes the release of soil phosphorus, improve containing for soil available phosphorus
Amount；On the other hand, citric acid and phosphate fertilizer reciprocation can significantly improve topsoil available phosphorus content, suitable citric acid and
Phosphate fertilizer proportioning can not only obtain high yield, but also can reduce Phosphorus fertilizer usage.
Potassium fulvate：Its action principle mainly includes the following aspects：(1) improve soil, potassium fulvate has with other
Machine colloid forms colloid substance in the soil, and soil particle cementation is got up, and increases water-stable granular structure；What potassium fulvate contained
A large amount of active groups are more, increase baseexchangecapacity, and absorption and the large number of deleterious cations of detention reduce soil salt
Concentration and acid-base value, so as to improve the ecological environment of crop.(2) fertilizer efficiency is improved, passes through the acting activating many minerals of exchange adsorption
Element improves fertilizer efficiency.The effect of nitrogenous fertilizer synergy is shown：Nitrogen volatilization loss is reduced, it is very aobvious to the synergistic effect of urea
It writes, can extend the fertilizer efficiency of urea, promote the absorption of nitrogen, improve utilization rate of nitrogen fertilizer.The synergistic effect of phosphate fertilizer is shown：Subtract
Few soil plays the role of phosphorus decomposing to quick-acting phosphorus fixations.It is available phosphorus to make insoluble phosphate-solubilizing in soil, promotes crops pair
The absorption of phosphorus.(3) promote crop growth, contain in potassium fulvate there are many functional group, as physiological activator, to crop
Growth and development and body physiological metabolism have stimulation, the active group of fulvic acid is (including carboxyl, phenolic hydroxyl group, quinonyl, hydroxyl
And certain groups containing P, O, N, S) be typically all electronq donor, it is easy to many electron acceptors (polyvalent metal from
Son, organic group or ion) such as the substance composition complex in earthworm hydrolyzate, referred to as complex compound or chelate.So as to
Be conducive to prevent the volatilization of nitrogen, be lost in, prevent P, K etc. by soil fixing, improve their validity and long-term effect or even Huang
Rotten acid is closely integrated into chemical bond with these nutrients, is absorbed and is utilized together by plant, so the utilization rate of nutrient can be improved.
Poly-aspartic-acid：Poly-aspartic-acid is negatively charged, and the poly-aspartic-acid of macromolecular can have negative ions
Certain suction-operated, it is especially preferable to the adsorption effect of nitrogen potassium.It can be combined, promoted with a variety of nutrient elements as fertilizer synergist
Nutrient is absorbed and utilized into crops, is plant nutrient sorbefacient.
The synergistic effect of each component is also embodied in the various substances of earthworm hydrolyzate and addition to promoting in inventive formulation
There is effective, significant coordination with the synthesis effect in terms of nitrogen phosphorus plant recovery of nutrient.Substance in earthworm hydrolyzate, including amino acid,
Antibacterial class substance, plant hormone etc. can improve crop immunocompetence, reduce pest and disease damage, and can promote plant growth, be crop
Growth provides nutrient, and the substance do not recognized plays positive facilitation.The phytase of addition, lemon
The substances such as acid, poly-aspartic-acid have the function of that obligate promotion nitrogen phosphorus is active, between the various substances of earthworm hydrolyzate and addition
In the presence of the mutual effect for cooperateing with, promoting, while the disease resistance for improving crop and the growth of crop can be promoted, promoted pair
The absorption of nitrogen P elements, it is therefore, more notable to the synergistic effect of nitrogen phosphorus.
The present invention has the advantages that compared with prior art：
(1) it improves N,P use efficiency, reduce nitrogen and phosphorous fertilizer use.It can be by nitrogen fertilizer use using nitrogen-phosphorus synergist of the present invention
Rate improves 25%~35%, and phosphate fertilizer utilization efficiency improves 20%~30%.It is average can save per acre nitrogen and phosphorous fertilizer using 35kg with
On.
(2) crop yield is improved.After practical planting experiment shows to apply nitrogen-phosphorus synergist of the present invention, reducing per acre
Under the premise of nitrogen and phosphorous fertilizer uses 35kg, crop yield can also be improved, averagely increases production more than 13%.
(3) improve soil quality.Enzyme Activities in Soils, polyphenol oxidase can be significantly improved using nitrogen-phosphorus synergist of the present invention
Enzyme and activity of acid phosphatase improve 6.78%~32.4%, and trace element effectively manganese content improves 23.0%, soil pH
Value is improved without significant changes, soil activation enhancing, available nutrient content.
(4) inhibit soil-borne disease.Graw mold of tomato incidence is made to reduce by 8% using nitrogen-phosphorus synergist of the present invention, root knot line
Insect infection diseased plant rate reduces, and controls 1%~3%.
Description of the drawings
Fig. 1 is that test example 1 is compared using nitrogen-phosphorus synergist of the present invention and the cucumber yield of conventional method.A, b are represented in figure
Difference reaches the level of signifiance (p between the different disposal of identical index<0.05).
Fig. 2 for test example 1 using nitrogen-phosphorus synergist of the present invention and conventional method in the cucumber best fruiting period, soil available nitrogen,
The content balance of available phosphorus.
Fig. 3 is that test example 2 is compared using nitrogen-phosphorus synergist of the present invention and the tomato yield of conventional method.
Fig. 4 for test example 2 using nitrogen-phosphorus synergist of the present invention and conventional method in the tomato best fruiting period, soil available nitrogen,
The content balance of available phosphorus.
Fig. 5 is that test example 2 is compared using nitrogen-phosphorus synergist of the present invention and the tomato incidence of conventional method.
Specific embodiment
With reference to specific embodiments and the drawings, technical scheme of the present invention and technique effect are done further specifically
It is bright.But protection scope of the present invention is not limited to following embodiments.
Embodiment 1
(1) earthworm hydrolyzate is prepared.Fresh earthworm 200g is taken, with sterile water wash 4 times, clean earthworm is placed at a high speed
In tissue mashing machine, 25min is smashed to pieces with the rotating speed of 4000r/min, is homogenized, by homogenate and water in mass ratio 1:3 are added to
In beaker, pH to 7 is adjusted with the dilute hydrochloric acid of a concentration of 0.02mol/L, adds 500mg complex enzyme (subtilopeptidase As：Spinach
Trailing plants protease=1:2) after, being stirred 4min, beaker is moved into heat-collecting magnetic stirring device, adjusts blender temperature to 42
DEG C, speed of agitator to 230r/min, enzyme digestion reaction 4.5h, enzymolysis terminates, enzymolysis liquid is put into centrifuge, with 4000r/min
Rotating speed centrifugation 20min, take upper liquid, i.e. earthworm hydrolyzate, Cord blood is spare.
(2) preparation of nitrogen-phosphorus synergist.By 18 parts of earthworm hydrolyzate, 5 parts of phytase, 17 parts of citric acid, potassium fulvate 40
Part, 23 parts of poly-aspartic-acid, 25 parts of water, are stirred nitrogen-phosphorus synergists of the 15~20min to get the hydrolyzate containing earthworm.
Test example 1：2016-2017 carries out nitrogen-phosphorus synergist application effect experiment in Shouguang City of Shandong Province facilities vegetable.
Green cucumber is carried out on for examination soil, is as follows：
Before plantation, to carrying out physicochemical property detection, soil pH value 7.3 for examination soil, quick-acting nitrogen contents are 138mg/kg,
Available phosphorus content is 233mg/kg, quick-acting potassium content 568mg/kg；1.5 mu of area, field planting on October 20th, 2016,2016 12
The moon uproots plants after their edible portions have been harvested on the 28th.Base manure applies 12~15 sides of Chicken dung/mu, 80kg/ mus of nitre sulfenyl fertilizer；Manuring late, with the change in growth period
Change, the type of top dressing changes therewith.The flushing and spreading fertilizer 1 time of fine day or 1 week hot day 1 time or 3~4 days；Rainy days, 10~15 Tianchong was applied
Fertilizer 1 time.Picking cucumber is recorded every time, summarizes the total output of cucumber, and (nutrient demand amount is most in the best fruiting period of cucumber
When big) acquisition pedotheque, detection Soil Available nitrogen, available phosphorus content.
Test group uses nitrogen-phosphorus synergist prepared by embodiment 1.Nitrogen-phosphorus synergist suggested use and dosage：During with each top dressing
Addition uses, and the cumulative volume of entire breeding time nitrogen-phosphorus synergist is 22.5kg/ mus, and accordingly reduce nitrogen phosphorus top dressing dosage.
Control group is applied fertilizer using conventional method.
Test group and control group after one batch of cucumber are planted, is as a result summarized as follows：
1) using the control group of the more conventional method of the test group of nitrogen-phosphorus synergist, cucumber yield improves 15.6% and (is averaged
Data) (as shown in Figure 1)；
2) using the control group of the more conventional method of the test group of nitrogen-phosphorus synergist, quick-acting nitrogen contents in soil have been respectively increased
35%th, available phosphorus content 20% (as shown in Figure 2)；
3) reduce nitrogen and phosphorous fertilizer dosage compared with the control group using conventional method using the test group of nitrogen-phosphorus synergist and add up to
36kg。
Embodiment 2
(1) earthworm hydrolyzate is prepared.Fresh earthworm 150g is taken, with sterile water wash 5 times, clean earthworm is placed at a high speed
In tissue mashing machine, 27min is smashed to pieces with the rotating speed of 4500r/min, is homogenized, by homogenate and water in mass ratio 1:2 are added to
In beaker, pH to 7.5 is adjusted with the dilute hydrochloric acid of a concentration of 0.02mol/L, adds 400mg complex enzyme (subtilopeptidase As：
Bromelain=1:3) after, being stirred 5min, will beaker move into heat-collecting magnetic stirring device in, adjust blender temperature to
40 DEG C, speed of agitator to 220r/min, enzyme digestion reaction 5h, enzymolysis terminates, enzymolysis liquid is put into centrifuge, with 4200r/min
Rotating speed centrifugation 25min, take upper liquid, i.e. earthworm hydrolyzate, Cord blood is spare.
(2) preparation of nitrogen-phosphorus synergist, by 20 parts of earthworm hydrolyzate, 5 parts of phytase, 20 parts of citric acid, potassium fulvate 50
Part, 25 parts of poly-aspartic-acid, 25 parts of water, are stirred nitrogen-phosphorus synergists of the 15~20min to get the hydrolyzate containing earthworm.
Test example 2：
Nitrogen-phosphorus synergist application effect experiment is carried out in Shandong Province orchid Ling County facilities vegetable within 2015.
Before plantation, to carrying out physicochemical property detection, soil pH value 7.3 for examination soil, quick-acting nitrogen contents are 121mg/kg,
Available phosphorus content is 189mg/kg, and quick-acting potassium content 368mg/kg, area is 5 mu, base manure fertilising (N：P：K=50:40:40kg/
Mu) and farm manure (4000 kgs/acre).
Test group uses nitrogen-phosphorus synergist prepared by embodiment 2, and the nitrogen, phosphorus synergist (20kg/ mus) are together with farm manure one
Play application.
Control group uses conventional method.
In early March, 2015 is colonized tomato；It uproots plants after their edible portions have been harvested at the beginning of 7 months 2015, the tomato meter of harvest produces 4500~5200kg/ mus,
And available nitrogen, available phosphorus content in soil are calculated in the best fruiting period, result is summarized as follows after tomato planting：
1) using the control group of the more conventional method of the test group of nitrogen-phosphorus synergist, tomato yield improves 13.0% (such as Fig. 3 institutes
Show)；
2) using the control group of the more conventional method of the test group of nitrogen-phosphorus synergist, quick-acting nitrogen contents in soil have been respectively increased
40%th, available phosphorus content 30% (as shown in Figure 4)；
3) using the test group of nitrogen-phosphorus synergist compared with the control group using conventional method, graw mold of tomato incidence reduces
8% (shown in Fig. 5), nematode infections diseased plant rate are reduced to 1%~3% from 5%.
Embodiment 3
(1) earthworm hydrolyzate is prepared.Fresh earthworm 100g is taken, with sterile water wash 3 times, clean earthworm is placed at a high speed
In tissue mashing machine, 28min is smashed to pieces with the rotating speed of 4200r/min, is homogenized, by homogenate and water in mass ratio 1:2 are added to
In beaker, pH to 7.5 is adjusted with the dilute hydrochloric acid of a concentration of 0.02mol/L, adds 300mg complex enzyme (subtilopeptidase As：
Bromelain=1:3) after, being stirred 3min, will beaker move into heat-collecting magnetic stirring device in, adjust blender temperature to
40 DEG C, speed of agitator to 200r/min, enzyme digestion reaction 4h, enzymolysis terminates, enzymolysis liquid is put into centrifuge, with 3800r/min
Rotating speed centrifugation 20min, take upper liquid, i.e. earthworm hydrolyzate.Earthworm hydrolyzate vacuum concentration is dried to pulvis.
(2) preparation of nitrogen-phosphorus synergist.By 18 parts of earthworm hydrolyzate pulvis, 5 parts of phytase, 17 parts of citric acid, fulvic acid
40 parts of potassium, 23 parts of poly-aspartic-acid, are thoroughly mixed, granule are conventionally made.
Claims (10)
1. a kind of nitrogen-phosphorus synergist of hydrolyzate containing earthworm, is counted in parts by weight, including 15~20 parts of earthworm hydrolyzate, phytase
4~5 parts, 15~20 parts of citric acid, 20~50 parts of potassium fulvate, 20~25 parts of poly-aspartic-acid.
2. the nitrogen-phosphorus synergist of the hydrolyzate containing earthworm as described in claim 1, it is characterized in that, the nitrogen-phosphorus synergist is liquid agent
Type, pulvis or granule.
3. the nitrogen-phosphorus synergist of the hydrolyzate containing earthworm as claimed in claim 1 or 2, it is characterized in that, it counts in parts by weight, including earthworm
18 parts of earthworm hydrolyzate, 5 parts of phytase, 17 parts of citric acid, 40 parts of potassium fulvate, 23 parts of poly-aspartic-acid, 20~30 parts of water.
4. a kind of preparation method of the nitrogen-phosphorus synergist of hydrolyzate containing earthworm, it is characterized in that, step is as follows：
(1) preparation of earthworm hydrolyzate：Fresh earthworm is smashed to pieces after cleaning and is homogenized, and adds in water and adjusts pH to 6~8, adds in
Complex enzyme carries out under the conditions of insulated and stirred digesting up to earthworm hydrolyzate；
(2) preparation of nitrogen-phosphorus synergist, by earthworm hydrolyzate, phytase, citric acid, potassium fulvate, poly-aspartic-acid and/or
Water is stirred nitrogen-phosphorus synergists of the 15~20min to get the hydrolyzate containing earthworm.
5. the preparation method of the nitrogen-phosphorus synergist of the hydrolyzate containing earthworm as claimed in claim 4, it is characterized in that, the complex enzyme
It is subtilopeptidase A and bromelain according to 1:2~3 ratios mix.
6. the preparation method of the nitrogen-phosphorus synergist of the hydrolyzate containing earthworm as described in claim 4 or 5, it is characterized in that, the step
Suddenly (1) takes fresh earthworm 100-200g, and with sterile water wash 3~5 times, clean earthworm is placed in high-speed tissue mashing machine,
25~30min is smashed to pieces with the rotating speed of 4000~5000r/min, is homogenized, by homogenate and water in mass ratio 1:2~1:4 add in
Into beaker, pH to 6~8 is adjusted with the dilute hydrochloric acid of a concentration of 0.02mol/L, adds 300~500mg complex enzymes, stirring is mixed
After closing 3~5min, beaker is moved into heat-collecting magnetic stirring device, adjusts blender temperature to 40~45 DEG C, speed of agitator is extremely
200~230r/min, 4~5h of enzyme digestion reaction, enzymolysis terminates, enzymolysis liquid is put into centrifuge, with 3500~4500r/min's
Rotating speed centrifuges 20~25min, takes upper liquid, i.e. earthworm hydrolyzate.
7. the preparation method of the nitrogen-phosphorus synergist of the hydrolyzate containing earthworm as described in claim 4-6 is any, it is characterized in that, step
(1) the earthworm hydrolyzate prepared, which is concentrated and dried, is prepared into pulvis into pulvis and other components mixing or is pressed further by being prepared into
Particle.
8. the nitrogen-phosphorus synergist of the hydrolyzate as described in any one of claims 1-3 containing earthworm improves nitrogen phosphorus fertilizer in agricultural production
Application in effect, reduction nitrogen phosphorus pollution of area source.
9. application as claimed in claim 8, it is characterized in that, the nitrogen-phosphorus synergist of the hydrolyzate containing earthworm is in facilities vegetable
In application.
10. the application described in claim 9, it is characterized in that, synergist amount of application in facilities vegetable is 20~30kg/
Mu.
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| CN109232093A (en) * | 2018-11-07 | 2019-01-18 | 天津天丰泽田生物科技有限公司 | A kind of high efficiency nutritious type Water soluble fertilizer |
| CN109293444A (en) * | 2018-12-05 | 2019-02-01 | 史丹利农业集团股份有限公司 | A kind of phosphorus element activator of calacareous soil and the preparation method and application thereof |
| CN110078560A (en) * | 2019-05-19 | 2019-08-02 | 邓灿辉 | A kind of preparation method of nitrogen-phosphorus synergist |
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