CN108118048A - Regulate and control the method and its application of Salmonella growth - Google Patents
Regulate and control the method and its application of Salmonella growth Download PDFInfo
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Abstract
The invention discloses a kind of method and its application of regulation and control Salmonella growth, and this method is that salmonella is carried out photo-irradiation treatment, wherein, in the case of the photo-irradiation treatment inhibits the growth of salmonella, the light quality of the photo-irradiation treatment is selected from blue light and/or yellow light；In the case of the photo-irradiation treatment promotes the growth of salmonella, the light quality of the photo-irradiation treatment is selected from green light and/or feux rouges.The method of the regulation and control Salmonella growth has the advantages that radiationless pollution risk, of low cost and easy to operate, and then can be applied in the occasion for needing Salmonella growth.
Description
Technical field
The present invention relates to the controls of bacterium, and in particular, to a kind of method and its application of regulation and control Salmonella growth.
Background technology
Salmonella (Salmonella) is a kind of common infecting both domestic animals and human pathogen, can not only cause animal typhoid fever, suddenly
Disorderly, the diseases such as human gastrointestinal inflammation, septicemia, the life and health of serious threat people, animal, the food security being induced by it are also resulted in
Event is in first of all food-borne pathogens.Salmonella nutritional requirement is low, food (meat, egg, milk, bread etc.), excrement,
Time-to-live length (5~12 months) in soil, water, and contaminated food is without significant change, easily eaten by mistake by animal and the mankind and
Trigger infectious diseases, animality disease mainly has avian typhoid, white diarrhea, hog cholera etc., human infection mainly trigger gastroenteritis,
The infection types such as parenteric fever, septicemia, flu are poisoned by food.It is shown according to the statistics of World Health Organization's issue in 2015, from
There is the food-borne intestines problem outburst of 5.82 hundred million 22 kinds of differences since 2010, cause more than 35 ten thousand people dead, wherein typhoid fever sramana
Salmonella 5.2 ten thousand, pathogenic escherichia coli 3.7 ten thousand, norovirus 3.5 ten thousand.Therefore, salmonella is food-borne cause
The monitored object of germ emphasis.Therefore, finding effectively inhibition Salmonella growth technology is particularly important.Traditional antibacterial master
To include chemicals method, biological prevention, physical control technology, it is specific as follows：
(Li Yang, Ju Yulin, Wang Xiaobo, Liu Ting Chinese medicines " even yellow " are to resistance to for traditional antibacterial general use chemicals method
Research [J] the Jilin animal and veterinary of medicine salmonella inhibitory action:2007,28(9)：9-11.；Jiang Yunbin, Li Xihong, Fan Xue
Logical, Tian Guangjuan, Li Limei, Pang Lingling are atomized plants essential oil to salmonella typhimurium inhibition and cherry and tomato quality
It is scientific and technological to influence [J] food industry, 2017,09:324-328.), with obvious effects, sterilization is quick, but process is complicated, cost
It is higher.
Biological prevention has good effect to the growth for inhibiting salmonella, but its process is complicated, fund consumption
Fei great, practical application also have certain distance (Yan Qigui, Wang Xin, Guo Wanzhu, Yuan Mengwei, Song Zhenhui, Yin Huaping, Lee's Jing, Cao Hong
Will, tri- bacillus of Chen Bin is to external antagonism research [J] the China Preventive Veterinary Medicine report of Salmonella, and 2007,
29(01):71-74)。
Measure of the body to salmonella immune system is improved, such as combines a variety of enteron aisles disease using manna oligosacchride or beneficial bacterium
Opportunistic pathogen reduces it and the adherency and promotion of enteron aisle is excreted, so as to reduce risk (the bright dragon sweet dews of food origin disease generation
Two Glyco inhabiting salmonellas and research [D] the Chinese Academy of Agricultural Sciences of E. coli adhesion, 2016. Li Qing inhibit salmonella
The screening of lactic acid bacteria and its to Caco-2 cellular defense mechanisms research [D] Agricultural University Of Nanjing, 2015)
Physical control technology is to utilize radiation：(Ma Haili, Han Keguang, Zheng Mingxue, Li Guozhu, bavin osmanthus is precious, Liu Guan for gamma-rays
Chapter .60Co gamma-rays is to radiation effect [J] nuclear agricultural science reports of Salmonella in high immunity egg yolk, 2002,16 (02):119-
121；) (Luo Wei, ultraviolet combination lactic acid bacteria handle application [D] tetra- in Fresh-cut Apples security control for ultraviolet light and coordination technique
River agriculture university, 2015), technical equipment requirement is high or processing cost is high, and with certain radiation pollution risk, is not suitable for
Large-scale commercial Application.
Therefore, how a kind of radiationless pollution risk, the method for regulation and control salmonella of low cost, easy to operate are provided
It is current urgent problem to be solved.
The content of the invention
The object of the present invention is to provide a kind of method and its application of regulation and control Salmonella growth, regulation and control salmonella lifes
Long method has the advantages that radiationless pollution risk, of low cost and easy to operate, and then can be applied to and need
In salmonella occasion.
To achieve these goals, the present invention provides a kind of method of regulation and control Salmonella growth, this method is by sand
Door Salmonella carries out photo-irradiation treatment, wherein, in the case of the photo-irradiation treatment inhibits the growth of salmonella, the photo-irradiation treatment
Light quality be selected from blue light and/or yellow light；In the case of the photo-irradiation treatment promotes the growth of salmonella, the photo-irradiation treatment
Light quality be selected from green light and/or feux rouges.
The present invention also provides a kind of application of method of above-mentioned regulation and control salmonella in salmonella occasion is regulated and controled.
In the above-mentioned technical solutions, the present invention by using photo-irradiation treatment mode so that inhibit salmonella growth,
Wherein, when the light quality of the photo-irradiation treatment is blue light and/or yellow light, the photo-irradiation treatment can inhibit the growth of salmonella；
When the light quality of the photo-irradiation treatment is green light and/or feux rouges, the photo-irradiation treatment can promote the growth of salmonella.This method
There is radiationless pollution risk, of low cost and easy to operate completely, so can apply it to needs regulate and control it is husky
In the occasion (such as food preservation) of door Salmonella.
Other features and advantages of the present invention will be described in detail in subsequent specific embodiment part.
Description of the drawings
Attached drawing is for providing a further understanding of the present invention, and a part for constitution instruction, with following tool
Body embodiment is together for explaining the present invention, but be not construed as limiting the invention.In the accompanying drawings：
Fig. 1 is blue group, yellow group, green group, red group, the colony growth situation map of CK group salmonellas.
Specific embodiment
The specific embodiment of the present invention is described in detail below.It is it should be appreciated that described herein specific
Embodiment is merely to illustrate and explain the present invention, and is not intended to limit the invention.
The endpoint of disclosed scope and any value are not limited to the accurate scope or value herein, these scopes or
Value should be understood to comprising the value close to these scopes or value.For numberical range, between the endpoint value of each scope, respectively
It between the endpoint value of a scope and individual point value and can be individually combined with each other between point value and obtain one or more
New numberical range, these numberical ranges should be considered as specific open herein.
The present invention provides a kind of method of regulation and control Salmonella growth, this method is to carry out salmonella at illumination
Reason, wherein, in the case of the photo-irradiation treatment inhibits the growth of salmonella, the light quality of the photo-irradiation treatment is selected from blue light
And/or yellow light；In the case of the photo-irradiation treatment promotes the growth of salmonella, the light quality of the photo-irradiation treatment is selected from green light
And/or feux rouges.
In the above-mentioned methods, the intensity of illumination of blue light can select in wide scope, but in order to make salmonella
Regulation and control have more significant effect, it is preferable that the intensity of illumination of blue light is 750-1550lx；
In the above method, in the case of the light quality of photo-irradiation treatment contains blue light, in order to which the regulation and control for making salmonella have
More significant effect, it is preferable that blue light also meets the following conditions：Radiant illumination is 40-50W/m2, S/P values are 19-21, related
Colour temperature is 90000-110000K, and peak wavelength scope is 430-470nm, and half-wave width is 19-22nm, dominant wavelength 430-
470nm。
In the above-mentioned methods, the intensity of illumination of yellow light can select in wide scope, but in order to make salmonella
Regulation and control have more significant effect, it is preferable that the intensity of illumination of yellow light is 7500-15300lx；
In the above method, in the case of the light quality of photo-irradiation treatment contains yellow light, in order to which the regulation and control for making salmonella have
More significant effect, it is preferable that yellow light also meets the following conditions：Radiant illumination is 30-36W/m2, S/P values are 1-1.2, related
Colour temperature is 3000-4000K, and peak wavelength scope is 530-590nm, and half-wave width is 110-115nm, dominant wavelength 550-590nm.
In the above-mentioned methods, the intensity of illumination of green light can select in wide scope, but in order to make salmonella
Regulation and control have more significant effect, it is preferable that the intensity of illumination of green light is 3000-6680lx；
In the above method, in the case of the light quality of photo-irradiation treatment contains green light, in order to which the regulation and control for making salmonella have
More significant effect, it is preferable that green light also meets the following conditions：Radiant illumination is 10-15W/m2, S/P values are 2-2.5, related
Colour temperature is 6000-6500K, and peak wavelength scope is 490-560nm, and half-wave width is 60-70nm, dominant wavelength 540-550nm.
In the above-mentioned methods, the intensity of illumination of feux rouges can select in wide scope, but in order to make salmonella
Regulation and control have more significant effect, it is preferable that the intensity of illumination of feux rouges is 900-1930lx；
In the above method, in the case of the light quality of photo-irradiation treatment contains feux rouges, in order to which the regulation and control for making salmonella have
More significant effect, it is preferable that feux rouges also meets the following conditions：Radiant illumination is 15-20W/m2, S/P values be 0.1-0.2, phase
Pass colour temperature is 950-1050K, and peak wavelength scope is 600-650nm, and half-wave width is 90-100nm, dominant wavelength 600-650nm.
In the above method, the time of photo-irradiation treatment can select in wide scope, but in order to make the tune of salmonella
Control has more significant effect, it is preferable that light application time is not less than 2h.
In order to cost-effective and improve work efficiency, it is highly preferred that light application time is 24-60h.
In the above method, the temperature of photo-irradiation treatment can select in wide scope, but in order to make the tune of salmonella
Control has more significant effect, it is preferable that the temperature of photo-irradiation treatment is 5-40 DEG C.
In the above-mentioned methods, the light presentation mode of photo-irradiation treatment has various, but from the economic viewpoint, it is preferable that
The light of photo-irradiation treatment is provided by LED light source.
In the present invention, the acquisition modes of salmonella can also select in wide scope, but in order to further
Improve the acquisition efficiency of salmonella, it is preferable that before illumination, this method further includes：First by the salmonella of 3-5 DEG C of preservation
Inoculation is cultivated 1-2 days in LB fluid nutrient mediums, then expands the bacterium solution dilution spread after culture to LB solid mediums
In cultivated.
The present invention also provides a kind of methods of above-mentioned regulation and control salmonella to need salmonella occasion (as eaten
Product preserve) in application.
The present invention will be described in detail by way of examples below.
Preparation example 1
The configuration of LB fluid nutrient mediums：10g tryptones, 5g yeast extracts, 10g NaCl；By Liquid Culture basigamy
Side weighs above-mentioned substance, adds deionized water 900ml, shakes to solute after being completely dissolved, with the NaOH solution tune pH of 5mol/L extremely
7.0；Then it is settled to 1L with deionized water；Then the steam sterilizing 20min under 121 DEG C of high pressures；Finally take 100mL Liquid Cultures
Base, it is spare.
Preparation example 2
The configuration of LB solid mediums：10g tryptones, 5g yeast extracts, 10g NaCl, 15g agar powders；By solid
Body culture medium prescription weighs above-mentioned substance, adds deionized water 900ml, is heated to after agar is completely dissolved, uses on the heaters
5mol/L NaOH solution tune pH is settled to 1L to 7.0, then with deionized water；Then steam sterilizing 20min under 121 DEG C of high pressures, when
It when temperature drops to about 55 DEG C, dispenses while hot, general 15ml falls 1 tablet；After culture medium is poured into culture dish, lid is opened,
It is ultraviolet lower according to 10-15 minutes；Finally preserve, with sealing adhesive edge, and be inverted be put in 4 DEG C of preservations, it is spare.
Embodiment 1
1) Salmonella strains that 4 DEG C of refrigerators preserve are taken out to be inoculated in LB fluid nutrient mediums, expands culture for 24 hours.
2) 1 milliliter of bacterium solution after cultivating will be expanded in second step, is added in 9 milliliters of sterile water, dilutes 10 times, then take
1 milliliter of bacterium solution diluted is added in 9 milliliters of sterile waters, is diluted to 10 6 powers times in this way, then is taken and diluted 6
0.1 milliliter of secondary bacterium solution, dilution spread are cultivated into prepared LB solid mediums.
3) culture is inverted with the blue light source irradiation of blue LED light, the method for plate culture count counts clump count.By blue LED light
Blue light be adjusted to intensity of illumination 1504lx (radiant illumination 46.6W/m2, S/P values are 20.9, correlated colour temperature 100000K, peak
Value wavelength is 444nm, and half-wave width is 21nm, dominant wavelength 450nm), in 37 DEG C of irradiations for 24 hours, it is denoted as blue group.
Embodiment 2
It is carried out according to the method for embodiment 1, except that：It is fallen in step 3) with the yellow light sources irradiation of yellow LED light
Culture is put, the method for plate culture count counts clump count.Yellow LED light is adjusted to intensity of illumination 15281lx (radiant illuminations with remote controler
For 35.7W/m2, S/P values are 1.1, and correlated colour temperature 3648K, peak wavelength 551nm, half-wave width are 113nm, and dominant wavelength is
571nm), 37 DEG C of cultures for 24 hours, are denoted as yellow group.
Embodiment 3
It is carried out according to the method for embodiment 1, except that：It is fallen in step 3) with the green-light source irradiation of green LED light
Culture is put, the method for plate culture count counts clump count.Green LED light is adjusted to intensity of illumination 6676lx (radiant illuminations with remote controler
For 14W/m2, S/P values are 2.2, and correlated colour temperature 6304K, peak wavelength 524nm, half-wave width are 65nm, and dominant wavelength is
545nm), 37 DEG C of cultures for 24 hours, are denoted as green group.
Embodiment 4
It is carried out according to the method for embodiment 1, except that：It is fallen in step 3) with the red-light source irradiation of red LED light
Culture is put, the method for plate culture count counts clump count.Red LED light is adjusted to intensity of illumination 1921lx (radiant illuminations with remote controler
For 17W/m2, S/P values are 0.17, and correlated colour temperature 1001K, peak wavelength 646nm, half-wave width are 95nm, and dominant wavelength is
617nm), 37 DEG C of cultures for 24 hours, are denoted as red group.
Embodiment 5
It is carried out according to the method for embodiment 1, except that：It is strong that the blue light of blue LED light is adjusted to illumination in step 3)
Spend 750lx (radiant illumination 40W/m2, S/P values are 19, correlated colour temperature 90000K, peak wavelength 430nm, and half-wave width is
19nm, dominant wavelength 434nm), irradiate 60h in 40 DEG C.
Embodiment 6
It is carried out according to the method for embodiment 1, except that：It is strong that the blue light of blue LED light is adjusted to illumination in step 3)
Spend 1550lx (radiant illumination 50W/m2, S/P values are 21, correlated colour temperature 110000K, peak wavelength 465nm, and half-wave is wide
For 22nm, dominant wavelength 470nm), irradiate 2h in 5 DEG C.
Embodiment 7
It is carried out according to the method for embodiment 1, except that：It is fallen in step 3) with the yellow light sources irradiation of yellow LED light
Culture is put, the method for plate culture count counts clump count.Yellow LED light is adjusted to intensity of illumination 7500lx (radiant illuminations with remote controler
For 30W/m2, S/P values are 1, and correlated colour temperature 3000K, peak wavelength 530nm, half-wave width are 110nm, and dominant wavelength is
550nm) 40 DEG C of culture 60h.
Embodiment 8
It is carried out according to the method for embodiment 1, except that：It is fallen in step 3) with the yellow light sources irradiation of yellow LED light
Culture is put, the method for plate culture count counts clump count.Yellow LED light is adjusted to intensity of illumination 15300lx (radiant illuminations with remote controler
For 36W/m2, S/P values are 1.2, and correlated colour temperature 4000K, peak wavelength 589nm, half-wave width are 115nm, and dominant wavelength is
590nm), 5 DEG C of culture 2h.
Embodiment 9
It is carried out according to the method for embodiment 1, except that：It is fallen in step 3) with the green-light source irradiation of green LED light
Culture is put, the method for plate culture count counts clump count.Green LED light is adjusted to intensity of illumination 3000lx (radiant illuminations with remote controler
For 10W/m2, S/P values are 2, and correlated colour temperature 6000K, peak wavelength 490nm, half-wave width are 60nm, and dominant wavelength is
540nm), 40 DEG C of culture 60h.
Embodiment 10
It is carried out according to the method for embodiment 1, except that：It is fallen in step 3) with the green-light source irradiation of green LED light
Culture is put, the method for plate culture count counts clump count.Green LED light is adjusted to intensity of illumination 6680lx (radiant illuminations with remote controler
For 15W/m2, S/P values are 2.5, and correlated colour temperature 6500K, peak wavelength 555nm, half-wave width are 70nm, and dominant wavelength is
550nm), 5 DEG C of culture 2h.
Embodiment 11
It is carried out according to the method for embodiment 1, except that：It is fallen in step 3) with the red-light source irradiation of red LED light
Culture is put, the method for plate culture count counts clump count.Red LED light is adjusted to intensity of illumination 900lx with remote controler, and (radiant illumination is
15W/m2, S/P values are 0.1, and correlated colour temperature 950K, peak wavelength 600nm, half-wave width are 90nm, dominant wavelength 600nm),
40 DEG C of culture 60h.
Embodiment 12
It is carried out according to the method for embodiment 1, except that：It is fallen in step 3) with the red-light source irradiation of red LED light
Culture is put, the method for plate culture count counts clump count.Red LED light is adjusted to intensity of illumination 1930lx (radiant illuminations with remote controler
For 20W/m2, S/P values are 0.2, and correlated colour temperature 1050K, peak wavelength 650nm, half-wave width are 100nm, and dominant wavelength is
650nm), 5 DEG C of culture 2h.
Comparative example 1
The procedure of Example 1 was followed except that in step 3)：It is inverted and cultivated with natural light irradiation, tablet
Colony counting method counts clump count.37 DEG C of cultures for 24 hours, are denoted as CK groups.
Interpretation of result
1) above-mentioned blue group, yellow group, green group, red group, the salmonella colony growth the result is shown in Figure 1s of CK groups (first row in figure
It is respectively yellow group, red group, CK groups from left to right, second row is respectively green group, blue group from left to right)；As shown in Figure 1, compare
The colony growth of group (CK groups) is good, and area of colony is big.Green group, red group, yellow group, blue group compared with CK groups, the growth feelings of bacterium colony
Condition is different, and wherein green light, having to salmonella for feux rouges group promote growth, and yellow light, blue light group have salmonella
Apparent rejection ability, wherein blue light group bacteriostasis are better than yellow light.
2) influence of the illumination to salmonella colony growth
After cultural method culture, every group of clump count is determined with colony counting method, is analyzed with SPSS13.0 softwares notable
Sex differernce the results are shown in Table 1.
Table 1
| Blue group | Yellow group | Green group | Red group | CK groups | |
| Clump count (107CFU/mL) | 0 | 39±15 | 177±32 | 167±26 | 116±27 |
It is in dramatically different that the bacterium colony of processing group and control group, which is sprouted, wherein blue group<Yellow group<CK groups<Red group of green group of ≈, warp
SPSS is analyzed, and processing group and control group are there are pole significant difference, wherein blue group is about the 1/100 of CK groups, Huang organizes about CK groups
It is 1/4, about the 1.3 of green group, red group CK group.Except green light between each experimental group, there was no significant difference for feux rouges outside, other are each
It is pole significant difference between group.
Embodiment 5-12 is detected in the same manner, wherein, embodiment 5-6 and the testing result of indigo plant group are basic
Unanimously, the testing result of embodiment 7-8 and yellow group is basically identical, and embodiment 9-10 and green group of testing result are basically identical, reality
The testing result that a 11-12 is applied with red group is basically identical.
The preferred embodiment of the present invention has been described above in detail, still, during present invention is not limited to the embodiments described above
Detail, within the scope of the technical concept of the present invention, a variety of simple variants can be carried out to technical scheme, this
A little simple variants all belong to the scope of protection of the present invention.
It is further to note that the specific technical features described in the above specific embodiments, in not lance
In the case of shield, can be combined by any suitable means, in order to avoid unnecessary repetition, the present invention to it is various can
The combination of energy no longer separately illustrates.
In addition, various embodiments of the present invention can be combined randomly, as long as it is without prejudice to originally
The thought of invention, it should also be regarded as the disclosure of the present invention.
Claims (10)
- A kind of 1. method of regulation and control Salmonella growth, which is characterized in that the method is that salmonella is carried out photo-irradiation treatment；Wherein, in the case of the photo-irradiation treatment inhibits the growth of salmonella, the light quality of the photo-irradiation treatment is selected from blue light And/or yellow light；In the case of the photo-irradiation treatment promotes the growth of salmonella, the light quality of the photo-irradiation treatment is selected from green light And/or feux rouges.
- 2. according to the method described in claim 1, wherein, the intensity of illumination of the blue light is 750-1550lx；Preferably, the blue light also meets the following conditions：Radiant illumination is 40-50W/m2, S/P values are 19-21, and correlated colour temperature is 90000-110000K, peak wavelength scope are 430-470nm, and half-wave width is 19-22nm, dominant wavelength 430-470nm.
- 3. according to the method described in claim 1, wherein, the intensity of illumination of the yellow light is 7500-15300lx；Preferably, the yellow light also meets the following conditions：Radiant illumination is 30-36W/m2, S/P values are 1-1.2, and correlated colour temperature is 3000-4000K, peak wavelength scope are 530-590nm, and half-wave width is 110-115nm, dominant wavelength 550-590nm.
- 4. according to the method described in claim 1, wherein, the intensity of illumination of the green light is 3000-6680lx；Preferably, the green light also meets the following conditions：Radiant illumination is 10-15W/m2, S/P values are 2-2.5, and correlated colour temperature is 6000-6500K, peak wavelength scope are 490-560nm, and half-wave width is 60-70nm, dominant wavelength 540-550nm.
- 5. according to the method described in claim 1, wherein, the intensity of illumination of the feux rouges is 900-1930lx；Preferably, the feux rouges also meets the following conditions：Radiant illumination is 15-20W/m2, S/P values be 0.1-0.2, correlated colour temperature For 950-1050K, peak wavelength scope is 600-650nm, and half-wave width is 90-100nm, dominant wavelength 600-650nm.
- 6. according to the method described in any one in claim 1-5, wherein, the time of the photo-irradiation treatment is not less than 2h.
- 7. according to the method described in any one in claim 1-5, wherein, the temperature of the photo-irradiation treatment is 5-40 DEG C.
- 8. according to the method described in any one in claim 1-5, wherein, the light of the photo-irradiation treatment is carried by LED light source For.
- 9. according to any one in claim 1-5, wherein, before the illumination, the method further includes：First will The Salmonella strains of 3-5 DEG C of preservation, which are inoculated in LB fluid nutrient mediums, to be cultivated 1-2 days, then expands the bacterium solution dilution after culture It is applied in LB solid mediums and is cultivated.
- 10. a kind of method of the regulation and control salmonella in 1-8 such as claim as described in any one is in regulation and control salmonella occasion In application.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111067007A (en) * | 2019-12-26 | 2020-04-28 | 上海海洋大学 | Method for killing salmonella through photodynamic |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111763603A (en) * | 2019-04-02 | 2020-10-13 | 周卓煇 | Use of light source for promoting fermentation and device for promoting fermentation |
| CN111067007A (en) * | 2019-12-26 | 2020-04-28 | 上海海洋大学 | Method for killing salmonella through photodynamic |
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