CN108112786A - Application of the cysteine as enteron aisle essential nutrients in animal health maintenance - Google Patents
Application of the cysteine as enteron aisle essential nutrients in animal health maintenance Download PDFInfo
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- CN108112786A CN108112786A CN201711367453.8A CN201711367453A CN108112786A CN 108112786 A CN108112786 A CN 108112786A CN 201711367453 A CN201711367453 A CN 201711367453A CN 108112786 A CN108112786 A CN 108112786A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/60—Feeding-stuffs specially adapted for particular animals for weanlings
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Abstract
The invention discloses application of the cysteine as enteron aisle essential nutrients in animal health maintenance.The present invention provides cysteine or cysteine preparation in the application as enteron aisle essential nutrients in animal health maintenance；The main component of the cysteine preparation is cysteine or the substance that cysteine can be converted into alimentary canal.Of the invention study finds that cysteine/cystine is enteron aisle essential amino acid, and the present invention provides effective nutrition technique measure to safeguard that animal intestinal tract is healthy with normal physiological function；The present invention provides a kind of than methionine supplement animal is utilized to need more effective ration preparation technology to cysteine/cystine in daily ration, scientific basis is provided for pig production efficiently, healthy.
Description
Technical field
The invention belongs to livestock-raisings and feed processing technology field, are related to cysteine and exist as enteron aisle essential nutrients
Application in animal health maintenance.
Background technology
Conventionally methionine is the essential amino acid of humans and animals, and methionine is in vivo except fulfiling own physiological work(
Outside energy, cysteine can also be converted into, and and then cystine is synthesized by dehydrogenation reaction.Cysteine plus cystine is in machine
It can mutually convert in vivo, but methionine cannot be converted into.Cysteine, cystine can using methionine as precursor convert and
Come, therefore also referred to as semi-dispensable amino acid.At present in animal feeding, typically made up by adding methionine in daily ration
The shortage of cysteine.
Animal alimentary canal often by it is various stress (such as weaning stress, reload stress, caused by temperature Change stress), disease
The invasion and attack of pathogenic microorganism and mycotoxin or even the reaction that is inflamed due to infection cause loss of appetite, diarrhea, alimentary canal work(
Energy disorder etc., has animal health and growth huge blow, therefore the fast quick-recovery animal alimentary canal of clinical means how to be coordinated to be good for
Health and normal physiological function have important economic value and animal welfare meaning.Particularly weanling piglet often occurs
Many disease of digestive tracts promote intestinal growth by Nutritional measures, and for improving the growth performance of piglet, it is outstanding to improve production efficiency
To be important.
External cause causes animal diarrhea, inflammation or stress reaction, and dysfunction occurs in alimentary canal, clinically usually requires
It is quick to check daily ration, the feedstuff that removal be toxic or harmful, and the drug for inhibiting microorganism is provided to animal intestinal tract, it gives
Enteron aisle anti-inflammatory supplements energy matter and recovers the remedy measures of ionic equilibrium to treat intestines problem.Research shows cysteine
The synthesis of the sulfur-bearings bioactive substances such as glutathione, taurine is participated in indirectly.But research of the cysteine in pig produces
It is less, and influence of the cysteine to piglet growth performance and intestinal growth does not have been reported that also.
The content of the invention
New method and a kind of new application of cysteine the object of the present invention is to provide a kind of animal intestinal tract health care.
Specifically, following several respects application provided by the invention all belongs to the scope of protection of the present invention：
First, cysteine or cysteine preparation as enteron aisle essential nutrients in animal health maintenance should
With.The main component of the cysteine preparation is cysteine or the object that cysteine can be converted into alimentary canal
Matter.
Wherein, the animal intestinal tract essential nutrients is used to supplement the scarce cysteine of animal intestinal tract institute.
Second, cysteine or cysteine preparation as or application in preparing following any product：Animal intestine
Road health product, auxiliary product, the auxiliary product for animal alimentary canal post-operative recovery for animal alimentary canal diseases treatment.
The main component of the cysteine preparation is cysteine or the substance that cysteine can be converted into alimentary canal.
Wherein, the product is used to supplement the scarce cysteine of animal intestinal tract institute.
3rd, cysteine or cysteine preparation as or application in preparing following any product：For promoting
Product into animal intestinal growth, the product for promoting growth of animal, the product for safeguarding animal health.The half Guang ammonia
The main component of acid supplement is cysteine or the substance that cysteine can be converted into alimentary canal.
Wherein, the product is used to supplement the scarce cysteine of animal intestinal tract institute.
In addition, the present invention also protects the following two kinds method：
First, it is a kind of supplement animal intestinal tract scarce cysteine method, be by being added in animal feed or daily ration
Cysteine or cysteine preparation are realized；Rather than by adding methionine in animal feed or daily ration come real
It is existing.The method of the present invention compensates for conventional method can not meet double of Guang of enteron aisle by adding methionine in animal feed or daily ration
The demand of propylhomoserin.
Second, a kind of method that vigor for alleviating animal intestinal tract cell caused by cysteine lacks declines, is to pass through
Cysteine or cysteine preparation or glutathione is added in into the cultivating system of the animal intestinal tract cell to realize；
Rather than it is realized by adding in methionine or Sodium Pyruvate into the cultivating system of the animal intestinal tract cell.
In the above described two methods, the main component of the cysteine preparation is cysteine or in alimentary canal
The substance of cysteine can be converted into.
It is above-mentioned it is each application and method in, the substance that cysteine can be converted into alimentary canal for it is any can
The substance of cysteine, such as cysteine salt, cystine or cystine salt can be converted into alimentary canal.
In above-mentioned each application and method, the animal includes but not limited to pig, such as weanling pig.At one of the present invention
In embodiment, the animal intestinal tract cell is specially swine intestinal epithelium cells, more specifically, such as IPEC-J2 cells.
In addition, a kind of feed for being used to raise pig or daily ration and its preparation method is also claimed in the present invention.
Described in raising the feed or daily ration of pig, standard ileal can to digest (Standardized Ileal
Digestible, SID) cysteine content be more than or equal to 0.32%.Wherein, % represents mass percentage.
Further, in described for raising the feed or daily ration of pig, SID methionine contents are 0.36%, SID, half Guang ammonia
Acid content is 0.32%.Wherein, % represents mass percentage.
The present invention demonstrates the required ammonia that cysteine is enteron aisle with swine intestinal epithelium cells IPEC-J2 cell models first
Base acid, cysteine shortage cause epithelial cell vigor to decline, and supplement methionine cannot alleviate cysteine shortage in culture medium
Caused cell viability declines, but above-mentioned effect can be alleviated by supplementing cysteine metabolin glutathione, should the result shows that
Cysteine is the function of promoting intestinal growth to play it by synthesizing glutathion and maintain intestinal epithelial cell vigor, and
And intestinal epithelial cell cannot be converted into cysteine using methionine.Therefore it is believed that when cysteine deficiency in daily ration
When, the required cysteine of animal intestinal tract is that methionine is converted into cysteine by liver, because liver has methionine to turn
Sulfenyl acts on relevant enzyme.Then, we design, have prepared the daily ration comprising different level methionine and cysteine, to feed
Piglet.It was found that it is respectively American science research committee (2012) (National to work as methionine and cysteine levels in daily ration
Research Council, NRC 2012) pig nutritional need recommended amounts when, piglet average daily gain highest, and certain level
Cysteine added with increase feed intake effect.It can be carried as chitling road essential amino acid so as to demonstrate cysteine
High growth of animal performance.The present invention has prompted effect of the cysteine/cystine as enteron aisle essential amino acid, to safeguard animal
Intestinal health and normal physiological function provide effective nutrition technique measure；The present invention provides a kind of than being utilized in daily ration
Methionine supplements animal needs more effective ration preparation technology to cysteine/cystine, for pig production efficiently, healthy
Provide scientific basis.
Description of the drawings
Fig. 1 is that cysteine lacks the effect added with methionine to pig jejunal epithelium cell viability.As a result represent with right
According to the relative level group as reference.As a result represented by mean+/-standard error, n=8.a-b：Different class means have difference
Shoulder mark is considered as significant difference (P<0.05).Processing one：Lack cysteine plus cystine in culture medium；Processing two：In processing one
Middle addition 0.2mmol/L methionine；Processing three：0.4mmol/L methionine is added in processing one；Processing four：In processing one
Add 0.8mmol/L methionine；Processing five：1mmol/L methionine is added in processing one.
Fig. 2 is that cysteine lacks the effect added with associated metabolites to pig jejunal epithelium cell viability.As a result table
Show the relative level using control group as reference.As a result represented by mean+/-standard error, n=8.a-b：Different class mean tools
There are different shoulder marks to be considered as significant difference (P<0.05).Processing one：Lack cysteine plus cystine in culture medium；Processing two：
0.2mmol/L glutathione is added in processing one；Processing three：0.2mmol/L Sodium Pyruvates are added in processing one；Processing four：
0.3mmol/L Sodium Pyruvates are added in processing one；Processing five：0.4mmol/L Sodium Pyruvates are added in processing one.
Specific embodiment
Experimental method used in following embodiments is conventional method unless otherwise specified.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
% in following embodiments is mass percentage unless otherwise specified.
DMEM-F12 culture mediums, antibiotic, trypsase and PBS, hyclone (Gibco companies, the U.S.).
Tissue Culture Plate (Corning companies, the U.S.).
DMEM culture mediums (US biological companies, the U.S.) without nonessential amino acid.
Cell counting Kit (CCK-8, Japanese colleague).
Amino acid, glutathione, Sodium Pyruvate (Sigma, the U.S.).
Pig jejunal epithelium cell IPEC-J2 (DSMZ companies, Germany).
Corn, dehulled soybean meal, expanded soybean, wheat bran, fish meal, whey powder, calcium bicarbonate, mountain flour, salt, piglet is in advance
Batch mixing：Purchased from Beijing Tongli Xingke Agricultural Technology Co., Ltd..
Feeding amino acid：Purchased from Beijing Jia Kang sources development in science and technology Co., Ltd.
CO2gas incubator (MCO-175)：Sanyo companies, Japan.
Multi-function microplate reader (SpectraMax M3)：Molecular Devices companies, the U.S..
Embodiment 1, cysteine lack the vigor for reducing pig jejunal epithelium cell IPEC-J2
First, the culture of IPEC-J2 cells
Inoculation 5,000~10,000IPEC-J2 cells add in 100 μ L DMEM-F12 in 96 orifice plates, per hole and cultivate completely
Base (the DMEM-F12 culture mediums of Gibco, add 10% hyclone and 1% antibiotic), in 37 DEG C of 5%CO2Incubator
Liquid is changed in middle culture every other day, sets blank control.
2nd, IPEC-J2 cell culture tests
When cell growth to 70% fusion, following processing is carried out：(1) cysteine plus cystine is lacked in culture medium
For 24 hours, (2) are lacking half for (only lacking cysteine plus cystine compared to described above " DMEM-F12 complete mediums ") processing
The processing of 0.2,0.4,0.8,1mmol/L methionine is added in cystine and cystine culture medium for 24 hours, (3) are lacking cysteine
With addition 0.2mmol/L glutathione in cystine culture medium or the processing of 0.2,0.3,0.4mmol/L Sodium Pyruvates for 24 hours.Every group
8 repetitions are designed, 10 μ L CCK-8 reagents are added in per hole, in 37 DEG C of 5%CO23h is reacted in incubator, is surveyed under microplate reader 450nm
Determine absorbance, which is proportionate with cell viability.
3rd, result of the test
As depicted in figs. 1 and 2, the vigor that cysteine significantly reduces swine intestinal epithelium cells is lacked, in cysteine
The methionine of various concentration is added in the case of shortage cannot alleviate under IPEC-J2 cell viabilities caused by cysteine lacks
Drop, but effect caused by cysteine lacks can be made up by adding glutathione.Illustrate that cysteine is thin for pig gut epithelium
The essential amino acid of born of the same parents, it is impossible to supplement its physiological function by supplementing methionine.And when supplement cysteine metabolin paddy
When the sweet peptide of Guang and Sodium Pyruvate, it can only alleviate swine intestinal epithelium cells caused by cysteine lacks when supplementing glutathione
Vigor declines.Therefore, cysteine is the essential amino acid of intestinal epithelial cell, and cysteine grows intestinal cell
Effect is mainly realized by synthesizing glutathion.
Embodiment 2, daily ration cysteine levels influence the growth of weanling pig
The weanling pig that 96 original body mass are 11 ± 0.28kg is selected in experiment, is randomly divided into 4 groups, every group 24.Four
Processing is respectively：(1) control group, daily ration SID methionine are that 0.48%, SID cysteines are 0.21%；(2) processing group one arrives
Three, daily ration SID methionine are 0.36% (2012 recommended amounts of NRC), and SID cysteines are respectively 0.21%, 0.32% (NRC
2012 recommended amounts *) and 0.43%.Ration Compound keeps all essential amino acid standards in daily ration to return with reference to NRC (2012)
The balance of intestinal digestion rate content, metabolic energy and electrolyte.Experimental animal free choice feeding and drinking-water, test period are 28 days.Experiment
For diet group into being shown in Table 1, trophic level is shown in Table 2.
Note：* recommended in 2012 standards of NRC " requirement of SID methionine " and " SID methionine and cysteine it is total
Requirement ", but without recommending " amount of SID cysteines ", by the total of the SID methionine recommended of NRC 2012 and cysteine
Amount is 0.32% after subtracting the amount of the SID methionine of the recommendations of NRC 2012, so " 0.32% is referred to as NRC in the present embodiment
The amount of the 2012 SID cysteines recommended ".
Table 1 tests daily ration composition (feeding basis)
Note：1Every kilogram of complete diet pellet provides 10000IU vitamin As, 1500IU vitamine D3s, 30IU vitamin Es, 2.5mg dimensions
Raw element K3,1.5mg vitamin B1s, 10mg vitamin B2s, 10mg vitamin B6s, 0.05mg vitamin B12s, 1mg folic acid, 0.5mg
Biotin, 30mg niacin, 20mg pantothenic acid, 20mg copper, 100mg iron, 110mg zinc, 40mg manganese, 0.3mg selenium, 0.54mg iodine, 100mg
Sweetener, 500mg flavouring agents, 100mg phytases.
2 diets of different nutrition level of table
Note：1STTD, the full enteron aisle digestibility of standard.
Such as table 3, the average daily gain of processing two (SID cysteines are 0.32%) and end are highest again, illustrate egg ammonia
Effect is most preferable when acid and cysteine meet respective requirement respectively；And the cysteine of certain level is adopted added with increase
The effect of appetite；Feed consumption weightening ratio does not have significant changes between each group.Illustrate that conventional method adds egg ammonia in animal diets
Acid can not meet the needs of animal is to cysteine.
3 ileum standard of table can digest influence of the cysteine levels to Growth Performance of Weaning Piglets
Note：1Processing refer to Multiple range test as a result, linear and secondary in a few days grain standard ileal can digest cysteine levels
Effect；Shoulder mark of going together is different to represent significant difference (P ＜ 0.05), n=6.
The result of integrated embodiment 1 and embodiment 2, it is seen that：Cysteine is the essential amino acid of swine intestinal epithelium cells,
When cysteine plus cystine lacks, intestinal epithelial cell vigor declines.Supplement methionine cannot alleviate cysteine and Guang
The decline of cytoactive caused by propylhomoserin lacks, but above-mentioned effect can be alleviated by supplementing cysteine metabolin glutathione.
Compared with traditional mixed feed methionine makes up the shortage of cysteine, methionine and cysteine are respectively that NRC 2012 recommends
During amount, the average daily gain of weanling pig and whole weight are optimal, illustrate cysteine as trophism essential amino acid energy
Enough improve the growth performance of weanling pig.
Claims (10)
1. the application of cysteine or cysteine preparation as enteron aisle essential nutrients in animal health maintenance；
The main component of the cysteine preparation is cysteine or the object that cysteine can be converted into alimentary canal
Matter.
2. application according to claim 1, it is characterised in that：The animal intestinal tract essential nutrients is used to supplement animal intestine
The scarce cysteine of road institute.
3. cysteine or cysteine preparation as or application in preparing following any product：Animal intestinal tract health care is produced
Product, auxiliary product, the auxiliary product for animal alimentary canal post-operative recovery for animal alimentary canal diseases treatment；
The main component of the cysteine preparation is cysteine or the object that cysteine can be converted into alimentary canal
Matter.
4. cysteine or cysteine preparation as or application in preparing following any product：For promoting animal intestine
Product, the product for promoting growth of animal, the product for safeguarding animal health of road development；
The main component of the cysteine preparation is cysteine or the object that cysteine can be converted into alimentary canal
Matter.
5. the application according to claim 3 or 4, it is characterised in that：The product lacks half Guang for supplementing animal intestinal tract
Propylhomoserin.
6. it is a kind of supplement animal intestinal tract scarce cysteine method, be by adding cysteine in animal feed or daily ration
Or cysteine preparation is realized；
The main component of the cysteine preparation is cysteine or the object that cysteine can be converted into alimentary canal
Matter.
7. a kind of method that vigor for alleviating animal intestinal tract cell caused by cysteine lacks declines is by being moved to described
Cysteine or cysteine preparation or glutathione is added in the cultivating system of object intestinal cell to realize；
The main component of the cysteine preparation is cysteine or the object that cysteine can be converted into alimentary canal
Matter.
8. according to any application or method in claim 1-7, it is characterised in that：The animal is pig.
9. according to any application or method in claim 1-8, it is characterised in that：The animal intestinal tract cell is chitling
Tract epithelial cell；
Specifically, the swine intestinal epithelium cells are swine intestinal epithelium cells IPEC-J2.
10. it is a kind of for raise the feed of pig or the preparation method of daily ration or by this method be formulated for raising pig
Feed or daily ration, it is characterised in that：Described in raising the feed or daily ration of pig, SID cysteine contents to be more than or equal to
0.32%；Wherein % represents mass percentage.
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| CN201711367453.8A CN108112786A (en) | 2017-12-18 | 2017-12-18 | Application of the cysteine as enteron aisle essential nutrients in animal health maintenance |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103478499A (en) * | 2013-10-12 | 2014-01-01 | 四川特驱投资集团有限公司 | Anti-diarrhea complete formula feed for piglets |
| CN104351562A (en) * | 2014-11-25 | 2015-02-18 | 武汉轻工大学 | Growth promoting agent for promoting growth of weaned pig and application |
| CN104398498A (en) * | 2014-12-04 | 2015-03-11 | 武汉轻工大学 | Application of N-acetyl-L-cysteine in preparing drug for treating or preventing porcine virus infection |
| CN106387317A (en) * | 2016-08-31 | 2017-02-15 | 上海绿博生物科技发展有限公司 | Microorganism feed additive capable of protecting pig livers and preparation method of microorganism feed additive |
-
2017
- 2017-12-18 CN CN201711367453.8A patent/CN108112786A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103478499A (en) * | 2013-10-12 | 2014-01-01 | 四川特驱投资集团有限公司 | Anti-diarrhea complete formula feed for piglets |
| CN104351562A (en) * | 2014-11-25 | 2015-02-18 | 武汉轻工大学 | Growth promoting agent for promoting growth of weaned pig and application |
| CN104398498A (en) * | 2014-12-04 | 2015-03-11 | 武汉轻工大学 | Application of N-acetyl-L-cysteine in preparing drug for treating or preventing porcine virus infection |
| CN106387317A (en) * | 2016-08-31 | 2017-02-15 | 上海绿博生物科技发展有限公司 | Microorganism feed additive capable of protecting pig livers and preparation method of microorganism feed additive |
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