CN108102970A - In a kind of removal cadmium pollution soil sour solvent cadmium and can reduction-state cadmium simultaneous foster type microbial function flora and its methods for making and using same - Google Patents
In a kind of removal cadmium pollution soil sour solvent cadmium and can reduction-state cadmium simultaneous foster type microbial function flora and its methods for making and using same Download PDFInfo
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Abstract
The invention discloses it is a kind of remove cadmium pollution soil in sour solvent cadmium and can reduction-state cadmium simultaneous foster type microbial function flora and its methods for making and using same, belong to microbial treatment heavy-metal contaminated soil technical field.The present invention is respectively adopted autotrophic type and heterotroph microorganism be re-dubbed it is a kind of can efficiently remove sour solvent in soil and can reduction-state cadmium simultaneous foster type microbial function flora, not only the Geobacter metallireducens, alicyclic acid bacillus, fixed nitrogen acetobacter, anaerobism slime bacteria and the trichoderma that belong to heterotrophic microorganism, but also Acidithiobacillus thiooxidans, thermophilic thiobacillus and bacillus acidophilus including belonging to autotrophic microbe are included.The problem of present invention not only solves the functional flora of the compounding bad adaptability in contaminated soil is added to, and difficulty colonizes, and ensure that in contaminated soil sour solvent and can reduction-state cadmium have higher removal rate, significantly reduce bioavailability of cadmium and Phyto-availability.The present invention has established certain basis to the popularization and application of microbial treatment cadmium pollution soil.
Description
Technical field
The present invention relates to it is a kind of for remove sour solvent in cadmium pollution soil and can reduction-state cadmium simultaneous foster type microorganism work(
Energy flora and its methods for making and using same belong to microorganism conversion and administer heavy-metal contaminated soil technical field.
Background technology
About 1.8~2.7 hundred million mu of the cultivated area of China's heavy metal pollution at present, be mainly distributed on south China Hunan, Jiangxi,
The provinces and regions such as Hubei, Sichuan, Guangxi, Guangdong, wherein cadmium pollution situation are particularly acute.Cadmium pollution region is concentrated mainly on mining, gold
Belong to smelting enterprise periphery, Irrigation District of Sewage, In The Suburbs of Large And Medium Cities and southern Acid Paddy Soils area etc..According to investigations, the exceeded food of cadmium has been
Through being related to grain, aquatic products, industrial crops, edible mushroom, pluck etc., illustrate to have compared in contaminated areas cadmium pollution situation
Generally.The continuous accumulation of soil cadmium content can reduce the diversity of nitrogen-fixing bacteria in soil, influence crop root to nitrogen fixation
And nitrate reductase activity；The increase of cadmium content can also destroy the chlorophyll in plant, reduce root system to moisture and nutrient
It absorbs.After cadmium element enters human body by food chain, the enzyme of functional protein in vivo with people and specificity reacts and makes it
It loses activity, and accumulation causes slow poisoning in the organs such as human kidney, liver, while also disturbs copper, cobalt, the micro member such as zinc
The eubolism of element in vivo, induces various diseases or even death.
Bioavailability of heavy metals and Phyto-availability are the weights for weighing effect of fertilizer migration and ecology influence
Parameter is wanted, while is played an important role in ecological geochemical is evaluated.Heavy Metals refer to a certain heavy metal element in environment
In with actual form existing for certain lewis' acid.Heavy metal ion enters as a kind of important pollutant in soil, meeting
By a series of reaction, including adsorbing, being complexed, leaching and reduction reaction etc., its actual existence form is made to have with input form
Institute is different.By these behaviors, different component of the heavy metal finally in a different manner with soil combines to form different shape
Heavy metal.By BCR extraction methods, Heavy Metals can be divided into weak acid extractable, can reduction-state, oxidable state and residue
State, wherein weak acid extractable and can reduction-state be easily absorbed and used by plants and human health caused to directly affect.Therefore, drop
The low weak acid extractable of heavy metal-polluted soil and can reduction-state content to reduce heavy metal-polluted soil pollution level, reduce heavy metal pair
The harm of human health and the influence of the ecosystem and population health are significant.
The content of the invention
The object of the present invention is to provide it is a kind of for remove sour solvent in cadmium pollution soil and can reduction-state cadmium simultaneous foster type
The preparation method of microbial function flora.Not only solve the functional flora of the compounding bad adaptability in contaminated soil is added to,
The problem of difficulty colonizes, and ensure that in contaminated soil sour solvent and can reduction-state cadmium have higher removal rate, significantly reduce
Bioavailability of cadmium and Phyto-availability have established certain basis to the popularization and application of microbial treatment cadmium pollution soil.
The purpose of the present invention is what is be accomplished by the following way.
In a kind of removal cadmium pollution soil sour solvent cadmium and can reduction-state cadmium simultaneous foster type microbial function flora preparation
Method：
The functional flora is by belonging to the Geobacter metallireducens (Geobacter of heterotrophic microorganism
Metallireducens), alicyclic acid bacillus (Alicyclobacillus sp.), fixed nitrogen acetobacter
(Acetobacter diazotrophicus), anaerobism slime bacteria (Anaeromyxobacter sp.), trichoderma
(Streptophyta sp.) and Acidithiobacillus thiooxidans (Acidithiobacillus for belonging to autotrophic microbe
Thiooxidans), thermophilic thiobacillus (Acidithiobacillus caldus), bacillus acidophilus (Acidiphilium
Cryptum) mix.
As a further improvement, the Geobacter metallireducens of heterotrophic microorganism, alicyclic acid bacillus, solid will first be belonged to
Nitrogen acetobacter, anaerobism slime bacteria and trichoderma compound to obtain mixed bacterial a；Belong to the acidophilus sulfur oxide sulphur bar of autotrophic microbe
Bacterium, thermophilic thiobacillus and bacillus acidophilus compound to obtain mixed bacterial b；Then mixed bacterial a and b are compounded to obtain mixed bacterial c,
It is finally further cultured for obtaining functional flora.
As a further improvement, first various microorganisms are individually cultivated, collects microorganism when reaching exponential phase
And count, the mixed bacterial for obtaining two kinds of nutrient types, wherein Geobacter metallireducens, fat are mixed according to micro organism quantity ratio
Naphthenic acid bacillus, fixed nitrogen acetobacter, anaerobism slime bacteria and trichoderma are according to 1~1.2：1~1.2：1~1.2：1~1.2：1~
1.2 ratio is mixed to get mixed bacterial a；Acidithiobacillus thiooxidans, thermophilic thiobacillus and bacillus acidophilus are according to 1~1.2：1
~1.5：1~1.2 ratio is mixed to get mixed bacterial b；By obtained mixed bacterial a and b according to micro organism quantity ratio 1:1 is mixed
It closes.
As a further improvement, the medium component that each microorganism is individually cultivated is：(NH4)2SO4, 0.3g/L；KCl,
0.01g/L；K2HPO4, 0.05g/L；MgSO4·7H2O, 0.05g/L and Ca (NO3)2, 0.001g/L；Individually culture sulphur reduction mud
When native bacillus, alicyclic acid bacillus, fixed nitrogen acetobacter, anaerobism slime bacteria and trichoderma, glucose 0.5 is additionally added in culture medium
~0.7g/L, 0.1~0.3g/L of dusty yeast；Individually when culture Acidithiobacillus thiooxidans, thermophilic thiobacillus and bacillus acidophilus,
0.5~1g/L of sulphur powder is added in culture medium.
As a further improvement, obtained mixed bacterial a and b are tamed to culture in culture medium containing cadmium respectively；Then
Mixed bacterial a and b are mixed to get mixed bacterial c, adaptability culture is finally carried out in culture medium containing cadmium obtains functional flora.
As a further improvement, obtained mixed bacterial a and b in culture medium is tamed are tamed respectively, tamed
Medium component is：(NH4)2SO4, 0.3g/L；KCl, 0.01g/L；K2HPO4, 0.05g/L；MgSO4·7H2O, 0.05g/L and
Ca(NO3)2, 0.001g/L；Cultivate mixed bacterial a when, in the medium add in 0.5~0.7g/L of glucose, dusty yeast 0.1~
0.3g/L；0.5~1g/L of sulphur powder is added in the medium when cultivating mixed bacterial b；In addition, caddy is added in the medium
150~200mol/L；The microorganism concn of passage inoculation is 1.2~2 × 10 every time7A/mL, the concentration of microorganism is extremely after culture
Reach 4 × 10 less8~109A/mL；After mixed bacterial a and b each at least cultivated for 20 generations, mixed according to micro organism quantity equal proportion
Obtain mixed bacterial c.
As a further improvement, the succeeding generations of mixed bacterial a and b carry out in the glass beaker of 2~3L, agitating paddle
Rotating speed is 400-500rpm, and cultivation temperature is 25~30 DEG C, and initial pH value is 3.0~4.5.
As a further improvement, the adaptability incubation of the mixed bacterial c is as follows：Minimal medium ingredient
For：(NH4)2SO4, 0.3g/L；KCl, 0.01g/L；K2HPO4, 0.05g/L；MgSO4·7H2O, 0.05g/L and Ca (NO3)2,
0.001g/L；Culture medium is additionally added 0.5~0.7g/L of glucose, 0.1~0.3g/L of dusty yeast, 0.5~1g/L of sulphur powder, caddy
150~200mol/L.
As a further improvement, adaptability culture is carried out to mixed bacterial c, succeeding generations are in the glass beaker of 2~3L
Middle progress, rotating speed of agitator are 400~500rpm, and cultivation temperature is 25~30 DEG C, and initial pH value is 3.0~4.5, is often commissioned to train foster
Time is 3~5 days, and when bacterium solution pH value reaches less than 2.0, microbial bacteria volume density reaches 4 × 108~109During a/mL, passed
It is commissioned to train foster, the microorganism concn of passage inoculation is 1.2~2 × 10 every time7A/mL.Mixed bacterial c is obtained after at least cultivating for 20 generations
And foster type microbial function flora.
It is a further object of the present invention to provide the above method be prepared can remove in cadmium pollution soil sour solvent cadmium and
Can reduction-state cadmium simultaneous foster type microbial function flora.
Third object of the present invention be to provide can remove sour solvent cadmium in cadmium pollution soil and can the simultaneous of reduction-state cadmium support
The application process of type microbial function flora：The microbial function flora is added to reaction in cadmium pollution soil and removes cadmium.
It is specific as follows：
100~200mL minimal mediums are added in 250~500mL conical flasks, medium component is：(NH4)2SO4,
0.3g/L；KCl, 0.01g/L；K2HPO4, 0.05g/L；MgSO4·7H2O, 0.05g/L；Ca(NO3)2, 0.001g/L；Glucose
0.5~0.7g/L；0.1~0.3g/L of dusty yeast；0.5~1g/L of sulphur powder；The grain size that 40~60wt% is added in this system is small
In the cadmium pollution soil that 20 mesh cadmium contents are 21mg/kg；Microbial function flora is added in conical flask, what is be initially added is micro-
Biological concentration is 1.2~2 × 107A/mL, rotating speed are 170~180rpm, and temperature is 25~30 DEG C, initial pH value for 3.0~
4.5, the reaction time is 3~5 days.
The present invention is directed to the microorganism conversion mechanism of Cadium In Polluted Soil form, is answered using the microorganism of Different Nutrition type
A kind of functional flora that can efficiently administer cadmium pollution soil is made into, the functional flora for not only solving compounding is being added to Polluted Soil
The problem of bad adaptability in earth, difficulty colonizes, and ensure that in contaminated soil sour solvent and can reduction-state cadmium have higher removal
Rate significantly reduces bioavailability of cadmium and Phyto-availability.
Description of the drawings
Fig. 1 be different mixed bacterials to sour solvent in soil and sour solvent and can reduction-state cadmium removal rate influence.
Specific embodiment
The present invention is further elaborated by the following examples, but not as a limitation of the invention.
There are the noxious material (heavy metal and other organic pollutions) of high concentration and a variety of nutrition classes in cadmium pollution soil
The indigenous microbes of type, will reduce bioavailability of cadmium and Phyto-availability in soil, and single bacterial strain is difficult heavy metal-polluted
Dye soil remediation field reaches industrial applications, a kind of adaptable therefore, it is necessary to build, and trophic structure is rationally and can will not
Solvent cadmium Efficient Conversion is soluble state cadmium and the microbial function flora of removal.
The present invention filters out first carries out pure culture to the multiple-microorganism that Cd fractionation has transformation function, further according to micro- life
The nutrient type of object constructs two kinds of functional floras of heterotrophism and autotrophy；Wherein, when building both floras, by same nutrition class
The bacterial strain of type carries out various combination and is mixed into several composite floras, reduces the antagonism between strain, plays their collaboration
Effect, and flora is tamed in culture medium containing cadmium.Then by several heterotrophism of structure and autotrophic microbe flora into
The reparative experiment of row cadmium pollution soil, picks out the best heterotrophism of repairing effect and autotrophy flora carries out equal proportion compounding.Wherein
Structure multiple heterotrophism and autotrophic microbe flora and removal soil in sour solvent and can reduction-state cadmium effect it is as follows：
1. heterotrophic microorganism functional flora：
Flora A：The ratios such as Geobacter metallireducens, alicyclic acid bacillus, fixed nitrogen acetobacter, anaerobism slime bacteria, trichoderma
Example mixing, after taming for 20 generations, repairing test show sour solvent in soil and can the removal rate of reduction-state cadmium be respectively 15.72% He
6.18%.
Flora B：The ratios such as fold candida bacterium, alicyclic acid bacillus, fixed nitrogen acetobacter, anaerobism slime bacteria, trichoderma
Example mixing, after taming for 20 generations, repairing test show sour solvent in soil and can the removal rate of reduction-state cadmium be respectively 13.23% He
5.11%.
Flora C：Geobacter metallireducens, pseudomonas aeruginosa, fixed nitrogen acetobacter, anaerobism slime bacteria, trichoderma equal proportion
Mixing, after taming for 20 generations, repairing test show sour solvent in soil and can the removal rate of reduction-state cadmium be respectively 6.34% He
7.69%.
Flora D：Geobacter metallireducens, alicyclic acid bacillus, sticky red rhodotorula, anaerobism slime bacteria, trichoderma equal proportion
Mixing, after taming for 20 generations, repairing test show sour solvent in soil and can the removal rate of reduction-state cadmium be respectively 11.94% He
6.16%.
Flora E：Geobacter metallireducens, alicyclic acid bacillus, fixed nitrogen acetobacter, Penicillium citrinum, trichoderma equal proportion are mixed
Close, after taming for 20 generations, repairing test show sour solvent in soil and can the removal rate of reduction-state cadmium be respectively 14.34% He
4.55%.2. autotrophic microbe functional flora：
Flora A：The sour bacterium equal proportion of Acidithiobacillus ferrooxidans strain GF, thermophilic thiobacillus, thermophilic acidophilus irony bacterium and ten thousand is mixed
Close, after taming for 20 generations, repairing test show sour solvent in soil and can the removal rate of reduction-state cadmium be respectively 14.27% He
4.12%.
Flora B：Acidithiobacillus thiooxidans, thermophilic thiobacillus, the mixing of bacillus acidophilus's equal proportion, after taming for 20 generations, are repaired
Experiments have shown that in soil sour solvent and can the removal rate of reduction-state cadmium be respectively 22.28% and 22.53%.
Flora C：Acidithiobacillus thiooxidans, thermophilic thiobacillus, the mixing of ferrous oxide hook end spirillum equal proportion, domestication 20
Dai Hou, repairing test show sour solvent in soil and can the removal rate of reduction-state cadmium be respectively 10.60% and 13.16%.
Heterotrophism flora A and autotrophy flora B is chosen as flora a and b according to result above, and is existed again to the two floras
Adaptability culture is carried out in culture medium containing cadmium, finally obtain can efficiently remove sour solvent in soil and and can reduction-state cadmium it is simultaneous
The type of supporting microbial function flora.
Cadmium pollution soil sample used in the present embodiment is taken from 4 kilometers of xiangtan, hunan province county Xiang River entrance, this area's Cadmium in Soil
It pollutes caused by the random discharge of industrial wastewater, the pollution time is long, and pollution level is serious, and difficulty of governance is big.After measured, in soil sample
Cadmium content is 21mg/kg, other heavy metal ion contents are less than national Specification GB 15618-1995《Soil environment quality
Standard》.
The microorganism that the present invention prepares functional flora is purchased from each culture presevation storehouse, respectively belongs to heterotrophic microorganism
Geobacter metallireducens (Geobacter metallireducens ATCC 53774), alicyclic acid bacillus
(Alicyclobacillus sp.CGMCC No.7038), fixed nitrogen acetobacter (Acetobacter diazotrophicus
CGMCC No.5455), anaerobism slime bacteria (Anaeromyxobacter sp.ATCC BAA258), trichoderma (Streptophyta
Sp.CGMCC No.8725) and belong to the Acidithiobacillus thiooxidans (Acidithiobacillus of autotrophic microbe
Thiooxidans ATCC19377), it is thermophilic thiobacillus (Acidithiobacillus caldus CCTCC AB207044), thermophilic
Acidfast bacilli (Acidiphilium cryptum CCTCC M208056) mixes.
The medium component that each microorganism is individually cultivated is：(NH4)2SO4, 0.3g/L；KCl, 0.01g/L；K2HPO4,
0.05g/L；MgSO4·7H2O, 0.05g/L and Ca (NO3)2, 0.001g/L；Individually culture Geobacter metallireducens, alicyclic acid bud
When spore bacillus, fixed nitrogen acetobacter, anaerobism slime bacteria and trichoderma, glucose 0.7g/L, dusty yeast 0.3g/L are added in culture medium；
Individually when culture Acidithiobacillus thiooxidans, thermophilic thiobacillus and bacillus acidophilus, sulphur powder 1g/L is added in the medium.It is various micro-
The cultivation temperature of biology is 30 DEG C, and constant incubator rotating speed is 500rpm, initial pH value 4.5.
When various microorganisms reach exponential phase, collect microorganism and count, mixed according to micro organism quantity ratio
Obtain the mixed bacterial of two kinds of nutrient types, wherein Geobacter metallireducens, alicyclic acid bacillus, fixed nitrogen acetobacter, detest
Oxygen slime bacteria and trichoderma are according to 1：1：1：1：1 ratio is mixed to get mixed bacterial a；Acidithiobacillus thiooxidans, the warm sulphur bar of happiness
Bacterium and bacillus acidophilus are according to 1：1：1.2 ratio is mixed to get mixed bacterial b.
Obtained mixed bacterial a and b are tamed in culture medium is tamed respectively, domestication medium component is：(NH4)2SO4, 0.3g/L；KCl, 0.01g/L；K2HPO4, 0.05g/L；MgSO4·7H2O, 0.05g/L and Ca (NO3)2, 0.001g/L；Training
When supporting mixed bacterial a, glucose 0.7g/L, dusty yeast 0.3g/L are added in the medium；In culture medium when cultivating mixed bacterial b
Middle addition sulphur powder 1g/L；In addition, caddy 200mol/L is added in the medium；The microorganism concn of passage inoculation is every time
2×107A/mL, the concentration of microorganism is at least up to 4 × 10 after culture9A/mL；Mixed bacterial a and b each at least cultivated for 20 generations
Afterwards, it is mixed to get mixed bacterial c according to micro organism quantity equal proportion.
The adaptability incubation that obtained mixed bacterial c is carried out carries out in the glass beaker of 2L, minimal medium into
It is divided into：(NH4)2SO4, 0.3g/L；KCl, 0.01g/L；K2HPO4, 0.05g/L；MgSO4·7H2O, 0.05g/L and Ca (NO3)2,
0.001g/L；Culture medium is additionally added glucose 0.7g/L, dusty yeast 0.3g/L, sulphur powder 1g/L, caddy 200mol/L.Agitating paddle
Rotating speed is 500rpm, initial pH value 4.5, is often 5 days for incubation time, when bacterium solution pH value reaches less than 2.0, microbial cells
Density reaches 4 × 109During a/mL, secondary culture is carried out 25 times, the microorganism concn of passage inoculation is 2 × 10 every time7A/mL.
100mL minimal mediums are added in 250mL conical flasks, ingredient is：(NH4)2SO4, 0.3g/L；KCl, 0.01g/
L；K2HPO4, 0.05g/L；MgSO4·7H2O, 0.05g/L；Ca(NO3)2, 0.001g/L；Glucose 0.7g/L；Dusty yeast 0.3g/
L；Sulphur powder 1g/L；In addition, the grain size that 50wt% is added in this system is less than the cadmium pollution soil that 20 mesh cadmium contents are 21mg/kg
Earth；By the mixed bacterial a after 20 generation of secondary culture and mixed bacterial b, the mixed bacterial group c (after domestication of non-secondary culture
Compound group a+b) and the obtained functional flora of adaptability culture of the present invention (having carried out domestication and adaptability culture) add respectively
Enter into conical flask, be 2 × 10 in the microorganism concn being initially added7A/mL, constant incubator rotating speed be 175rpm, temperature
For 30 DEG C, initial pH value is compared experiment under the conditions of being 4.5, and the reaction time is 5 days.
As shown in Figure 1, sour solvent cadmium and oxidable state cadmium removal rate of the functional flora disclosed by the invention to contaminated soil
Reach 87.17% and 63.20%, and utilize mixed bacterial c (only mixed bacterial a+b), the passage for not carrying out adaptability culture
The cadmium sour solvent of mixed bacterial a and mixed bacterial b pollution administration soil after domestication culture and oxidable state cadmium removal rate difference
Only 38.05% and 43.41%, 15.72% and 6.18%, 22.28% and 22.53%.
Claims (10)
1. it is a kind of remove cadmium pollution soil in sour solvent cadmium and can reduction-state cadmium simultaneous foster type microbial function flora preparation side
Method, which is characterized in that the functional flora is by belonging to the Geobacter metallireducens (Geobacter of heterotrophic microorganism
Metallireducens), alicyclic acid bacillus (Alicyclobacillus sp.), fixed nitrogen acetobacter
(Acetobacter diazotrophicus), anaerobism slime bacteria (Anaeromyxobacter sp.), trichoderma
(Streptophyta sp.) and Acidithiobacillus thiooxidans (Acidithiobacillus for belonging to autotrophic microbe
Thiooxidans), thermophilic thiobacillus (Acidithiobacillus caldus), bacillus acidophilus (Acidiphilium
Cryptum) mix.
2. it is according to claim 1 it is a kind of remove cadmium pollution soil in sour solvent cadmium and can reduction-state cadmium the simultaneous foster micro- life of type
The preparation method of object functional flora, which is characterized in that will first belong to Geobacter metallireducens, the alicyclic acid gemma of heterotrophic microorganism
Bacillus, fixed nitrogen acetobacter, anaerobism slime bacteria and trichoderma compound to obtain mixed bacterial a；Belong to the acidophilus oxidation of autotrophic microbe
Sulphur Thiobacillus, thermophilic thiobacillus and bacillus acidophilus compound to obtain mixed bacterial b；Then mixed bacterial a and b are compounded and is mixed
Flora c is finally further cultured for obtaining functional flora.
3. it is according to claim 2 it is a kind of remove cadmium pollution soil in sour solvent cadmium and can reduction-state cadmium the simultaneous foster micro- life of type
The preparation method of object functional flora, which is characterized in that first various microorganisms are individually cultivated, are collected when reaching exponential phase
Microorganism simultaneously counts, and the mixed bacterial for obtaining two kinds of nutrient types, wherein sulphur reduction soil are mixed according to micro organism quantity ratio
Bacillus, alicyclic acid bacillus, fixed nitrogen acetobacter, anaerobism slime bacteria and trichoderma are according to 1~1.2：1~1.2：1~1.2：1~
1.2：1~1.2 ratio is mixed to get mixed bacterial a；Acidithiobacillus thiooxidans, thermophilic thiobacillus and bacillus acidophilus are according to 1
~1.2：1~1.5：1~1.2 ratio is mixed to get mixed bacterial b；By obtained mixed bacterial a and b according to micro organism quantity
Than 1:1 mixing.
4. it is according to claim 3 it is a kind of remove cadmium pollution soil in sour solvent cadmium and can reduction-state cadmium the simultaneous foster micro- life of type
The preparation method of object functional flora, which is characterized in that the medium component that each microorganism is individually cultivated is：(NH4)2SO4, 0.3g/
L；KCl, 0.01g/L；K2HPO4, 0.05g/L；MgSO4·7H2O, 0.05g/L and Ca (NO3)2, 0.001g/L；Individually culture sulphur
When reducing soil bacillus, alicyclic acid bacillus, fixed nitrogen acetobacter, anaerobism slime bacteria and trichoderma, Portugal is additionally added in culture medium
Grape 0.5~0.7g/L of sugar, 0.1~0.3g/L of dusty yeast；Individually culture Acidithiobacillus thiooxidans, thermophilic thiobacillus and acidophilus bar
During bacterium, 0.5~1g/L of sulphur powder is added in the medium.
5. it is according to claim 2 it is a kind of remove cadmium pollution soil in sour solvent cadmium and can reduction-state cadmium the simultaneous foster micro- life of type
The preparation method of object functional flora, which is characterized in that obtained mixed bacterial a and b are tamed to training in culture medium containing cadmium respectively
It supports；Then mixed bacterial a and b are mixed to get mixed bacterial c, adaptability culture is finally carried out in culture medium containing cadmium obtains work(
It can flora.
6. it is according to claim 5 it is a kind of remove cadmium pollution soil in sour solvent cadmium and can reduction-state cadmium the simultaneous foster micro- life of type
The preparation method of object functional flora, which is characterized in that tame and docile obtained mixed bacterial a and b in culture medium is tamed respectively
Change, domestication medium component is：(NH4)2SO4, 0.3g/L；KCl, 0.01g/L；K2HPO4, 0.05g/L；MgSO4·7H2O,
0.05g/L and Ca (NO3)2, 0.001g/L；When cultivating mixed bacterial a, 0.5~0.7g/L of glucose, ferment are added in the medium
0.1~0.3g/L of female powder；0.5~1g/L of sulphur powder is added in the medium when cultivating mixed bacterial b；In addition, in the medium
Add in 150~200mol/L of caddy；The microorganism concn of passage inoculation is 1.2~2 × 10 every time7A/mL, micro- life after culture
The concentration of object is at least up to 4 × 108~109A/mL；After mixed bacterial a and b each at least cultivated for 20 generations, according to micro organism quantity
Equal proportion is mixed to get mixed bacterial c；
The adaptability incubation of the mixed bacterial c is as follows：Minimal medium ingredient is：(NH4)2SO4, 0.3g/L；KCl,
0.01g/L；K2HPO4, 0.05g/L；MgSO4·7H2O, 0.05g/L and Ca (NO3)2, 0.001g/L；Culture medium is additionally added grape
Sugar 0.5~0.7g/L, 0.1~0.3g/L of dusty yeast, 0.5~1g/L of sulphur powder, 150~200mol/L of caddy；When being often commissioned to train foster
Between for 3~5 days, when bacterium solution pH value reaches less than 2.0, microbial bacteria volume density reaches 4 × 108~109During a/mL, passed on
Culture, the microorganism concn of passage inoculation is 1.2~2 × 10 every time7A/mL.Mixed bacterial c at least cultivate 20 generations obtain and support
Type microbial function flora.
7. in a kind of removal cadmium pollution soil according to right wants 5 sour solvent cadmium and can reduction-state cadmium simultaneous foster type microorganism
The preparation method of functional flora, which is characterized in that the succeeding generations of mixed bacterial a and b carry out in the glass beaker of 2~3L,
Rotating speed of agitator is 400-500rpm, and cultivation temperature is 25~30 DEG C, and initial pH value is 3.0~4.5；
Adaptability culture is carried out to mixed bacterial c, succeeding generations carry out in the glass beaker of 2~3L, rotating speed of agitator 400
~500rpm, cultivation temperature are 25~30 DEG C, and initial pH value is 3.0~4.5.
8. it is a kind of remove sour solvent cadmium in cadmium pollution soil and can reduction-state cadmium simultaneous foster type microbial function flora, feature exists
In being the microbial function flora being prepared by claim 1~7 any one of them method.
9. described in claim 8 removal cadmium pollution soil in sour solvent and can reduction-state cadmium simultaneous foster type microbial function flora
Application process, which is characterized in that the microbial function flora is added in cadmium pollution soil reaction except cadmium.
10. it is according to claim 9 removal cadmium pollution soil in sour solvent and can reduction-state cadmium simultaneous foster type microorganism work(
The application process of energy flora：
100~200mL minimal mediums are added in 250~500mL conical flasks, medium component is：(NH4)2SO4, 0.3g/
L；KCl, 0.01g/L；K2HPO4, 0.05g/L；MgSO4·7H2O, 0.05g/L；Ca(NO3)2, 0.001g/L；Glucose 0.5~
0.7g/L；0.1~0.3g/L of dusty yeast；0.5~1g/L of sulphur powder；The grain size that 40~60wt% is added in this system is less than 20 mesh
Cadmium content is the cadmium pollution soil of 21mg/kg；Microbial function flora is added in conical flask, the microorganism being initially added is dense
It spends for 1.2~2 × 107/mL, rotating speed is 170~180rpm, and temperature is 25~30 DEG C, and initial pH value is 3.0~4.5, reaction
Time is 3~5 days.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110724649A (en) * | 2019-10-09 | 2020-01-24 | 四川大学 | Efficient bioleaching composite bacterial system and application thereof in sludge dechromization process |
| CN110832966A (en) * | 2019-11-25 | 2020-02-25 | 辽宁大学 | Method for improving soil fertility by using compound microbial agent |
| CN114410550A (en) * | 2022-03-15 | 2022-04-29 | 中南大学 | Microbial functional flora for increasing flavonoid glycoside content in ginkgo leaves and preparation and application thereof |
| CN114958822A (en) * | 2022-06-09 | 2022-08-30 | 南京工业大学 | Method for constructing biological adhesion immobilized mycoderm by using sticky bacteria and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106984646A (en) * | 2017-04-17 | 2017-07-28 | 湖南省农业生物技术研究中心 | A kind of cadmium pollution soil for adapting to high solid-liquid ratio system administers the application of composite bacteria agent |
| CN107020295A (en) * | 2017-06-14 | 2017-08-08 | 中南大学 | A kind of cadmium pollution arable land in-situ rapid renovation method based on microorganism conversion |
| CN107099298A (en) * | 2017-06-14 | 2017-08-29 | 湖南省农业生物技术研究中心 | A kind of energy converted in-situ administers the application process of the microbial bacterial agent in cadmium pollution arable land |
| CN107189957A (en) * | 2017-04-17 | 2017-09-22 | 中南大学 | A kind of cadmium pollution soil for adapting to high solid-liquid ratio system administers composite bacteria agent and preparation method thereof |
-
2018
- 2018-01-23 CN CN201810065127.XA patent/CN108102970B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106984646A (en) * | 2017-04-17 | 2017-07-28 | 湖南省农业生物技术研究中心 | A kind of cadmium pollution soil for adapting to high solid-liquid ratio system administers the application of composite bacteria agent |
| CN107189957A (en) * | 2017-04-17 | 2017-09-22 | 中南大学 | A kind of cadmium pollution soil for adapting to high solid-liquid ratio system administers composite bacteria agent and preparation method thereof |
| CN107020295A (en) * | 2017-06-14 | 2017-08-08 | 中南大学 | A kind of cadmium pollution arable land in-situ rapid renovation method based on microorganism conversion |
| CN107099298A (en) * | 2017-06-14 | 2017-08-29 | 湖南省农业生物技术研究中心 | A kind of energy converted in-situ administers the application process of the microbial bacterial agent in cadmium pollution arable land |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110724649A (en) * | 2019-10-09 | 2020-01-24 | 四川大学 | Efficient bioleaching composite bacterial system and application thereof in sludge dechromization process |
| CN110724649B (en) * | 2019-10-09 | 2021-07-09 | 四川大学 | Efficient bioleaching composite bacterial system and application thereof in sludge dechromization process |
| CN110832966A (en) * | 2019-11-25 | 2020-02-25 | 辽宁大学 | Method for improving soil fertility by using compound microbial agent |
| CN114410550A (en) * | 2022-03-15 | 2022-04-29 | 中南大学 | Microbial functional flora for increasing flavonoid glycoside content in ginkgo leaves and preparation and application thereof |
| CN114958822A (en) * | 2022-06-09 | 2022-08-30 | 南京工业大学 | Method for constructing biological adhesion immobilized mycoderm by using sticky bacteria and application thereof |
| CN114958822B (en) * | 2022-06-09 | 2023-10-27 | 南京工业大学 | Method for constructing bioadhesive immobilized bacterial film by using myxobacteria and application of bioadhesive immobilized bacterial film |
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