CN108102968A - A kind of wheat planting is with microorganism and preparation method thereof - Google Patents
A kind of wheat planting is with microorganism and preparation method thereof Download PDFInfo
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- CN108102968A CN108102968A CN201810040031.8A CN201810040031A CN108102968A CN 108102968 A CN108102968 A CN 108102968A CN 201810040031 A CN201810040031 A CN 201810040031A CN 108102968 A CN108102968 A CN 108102968A
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- microorganism
- wheat planting
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Forests & Forestry (AREA)
- Ecology (AREA)
- Biotechnology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Environmental Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Pest Control & Pesticides (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to field of agricultural microbial technology, especially a kind of wheat planting can form the poly- Vetsin of the superfine natural material of intensity with microorganism, be the protective film of soil, prevent fertile part and moisture loss；While it is proliferated, the amylase of high activity can be disengaged, the macromolecular substances that hardly possible is decomposed are resolved into available small-molecule substance.The invention also discloses a kind of wheat planting microorganism preparation methods, comprise the following steps：S1, strain is heat-treated 5 20 minutes with after normal saline dilution through 80 DEG C of water-baths；S2, S3 on plating medium, condition of culture are coated on：32 35 DEG C, 150 180r/min of temperature cultivates 26h；S4, the fermentation medium that the culture solution Jing Guo previous step is transferred to fermentation tank；Invention, which can be realized, is prepared on a large scale the microorganism for being beneficial to wheat planting, contributes to wheat planting, can improve wheat yield.
Description
Technical field
The present invention relates to field of agricultural microbial technology more particularly to a kind of wheat planting microorganism and its preparation sides
Method.
Background technology
Wheat is the general designation of wheat system plant, is monocotyledon, is a kind of grass family planted extensively all over the world
Plant, the caryopsis of wheat is one of staple food of the mankind, and the foods such as bread, steamed bun, biscuit, noodles can be made after wearing into flour；Hair
It can be made into beer, alcohol, white wine (such as vodka) or raw matter fuel after ferment.Wheat is rich in starch, protein, fat, mineral
Matter, calcium, iron, thiamine, riboflavin, niacin, vitamin A and vitamin C etc..
With the research of rhizosphere microorganism, it has been found that plant rhizosphere exists and has biological nitrogen fixation, Soluble phosphorus, potassium decomposing and secretion
The bacterial strain of plant hormone.In general, rhizosphere growth-promoting endophytic bacteria refers to that existence in plant roots circle scope, has promotion to plant growth
Or the beneficial bacterium for having antagonism to pathogen is referred to as, the effect and mechanism for promoting plant growth generally may be summarized to be
The following aspects：(1) plant growth-promoting substance is secreted；(2) nutrient environment of plant rhizosphere is improved；(3) to the life of crop disease
Object control action；(4) to degradation of pollutant etc..
However the method for lacking the microorganism for being capable of wheat growth-promoting in the prior art and preparing this kind of microorganism.
The content of the invention
The purpose of the present invention is to solve shortcoming in the prior art, and a kind of micro- life of the wheat planting proposed
Object and preparation method thereof.
To achieve these goals, present invention employs following technical solutions：
A kind of wheat planting is designed with microorganism, the poly- Vetsin of the superfine natural material of intensity can be formed, be
The protective film of soil prevents fertile part and moisture loss；While it is proliferated, the amylase of high activity can be disengaged, hardly possible is decomposed
Macromolecular substances resolve into available small-molecule substance.
Preferably, the substances such as a variety of organic acids, enzyme, physiological activity can be synthesized and other a variety of be easily utilized
Nutrient.
Preferably, it is antibacterial, harmful power of going out is strong：With the advantage of taking up space, inhibit the harmful microorganisms such as harmful bacteria, pathogen
Growth and breeding
Preferably, organic substance, organic sulfur compound, organic nitrogen for generating foul gas etc. can be decomposed, is substantially improved
The environment in place.
Preferably, bacterium, Bacillaceae are belonged to.
Preferably, bacillus is belonged to.
The invention also discloses a kind of wheat planting microorganism preparation methods, comprise the following steps：
S1, will be heat-treated 5-20 minutes through 80 DEG C of water-baths after strain normal saline dilution；
S2, it is coated on plating medium, the constituent (g/L) of culture medium：Peptone 6-12, sodium chloride 8-
12, beef extract 3-5, pH value 7.0-7.5；
S3, condition of culture：32-35 DEG C, 150-180r/min of temperature cultivates 26h；
S4, the fermentation medium that the culture solution Jing Guo previous step is transferred to fermentation tank in 30-37 DEG C of temperature, stir 150-
It ferments under the conditions of 200r/min；
Fermentation medium components (g/L)：Peptone 18-20, yeast extract 7-12, glucose 18-22, disodium hydrogen phosphate 3-
5, potassium dihydrogen phosphate 3-5, manganese sulfate 0.4-0.6, calcium chloride 0.2-0.5.
Preferably, by 7g peptones, 10g sodium chloride in S2, the mixing of 4g beef extracts adds in 1L distilled water, then at 125 DEG C
High pressure sterilization obtains culture medium.
A kind of wheat planting proposed by the present invention is with microorganism and preparation method thereof, advantageous effect：The present invention can
It realizes the microorganism for being prepared on a large scale and being beneficial to wheat planting, contributes to wheat planting, wheat yield can be improved.
Specific embodiment
The technical solution in the embodiment of the present invention is clearly and completely described below, it is clear that described embodiment
Only part of the embodiment of the present invention, instead of all the embodiments.
Embodiment 1
A kind of wheat planting can form the poly- Vetsin of the superfine natural material of intensity with microorganism, be soil
Protective film, prevent fertile part and moisture loss；While it is proliferated, the amylase of high activity can be disengaged, hardly possible is decomposed big
Molecular substance resolves into available small-molecule substance.
It can synthesize the substances such as a variety of organic acids, enzyme, physiological activity and other a variety of nutrients being easily utilized.
Its is antibacterial, harmful power of going out is strong：With the advantage of taking up space, inhibit the growth of the harmful microbes such as harmful bacteria, pathogen
Breeding
It can decompose organic substance, organic sulfur compound, organic nitrogen for generating foul gas etc., substantially improve the ring in place
Border.
It belongs to bacterium, Bacillaceae.
It belongs to bacillus.
The invention also discloses a kind of wheat planting microorganism preparation methods, comprise the following steps：
S1, will be heat-treated 5 minutes through 80 DEG C of water-baths after strain normal saline dilution；
S2, it is coated on plating medium, the constituent (g/L) of culture medium：Peptone 6, sodium chloride 8, beef
Cream 3, pH value 7.0；
S3, condition of culture：32 DEG C, 150r/min of temperature cultivates 26h；
S4, the fermentation medium that the culture solution Jing Guo previous step is transferred to fermentation tank in 30 DEG C of temperature, stir 150r/min
Under the conditions of ferment；
Fermentation medium components (g/L)：Peptone 18, yeast extract 7, glucose 18, disodium hydrogen phosphate 3, biphosphate
Potassium 3, manganese sulfate 0.4, calcium chloride 0.2.
Embodiment 2
A kind of wheat planting can form the poly- Vetsin of the superfine natural material of intensity with microorganism, be soil
Protective film, prevent fertile part and moisture loss；While it is proliferated, the amylase of high activity can be disengaged, hardly possible is decomposed big
Molecular substance resolves into available small-molecule substance.
It can synthesize the substances such as a variety of organic acids, enzyme, physiological activity and other a variety of nutrients being easily utilized.
Its is antibacterial, harmful power of going out is strong：With the advantage of taking up space, inhibit the growth of the harmful microbes such as harmful bacteria, pathogen
Breeding
It can decompose organic substance, organic sulfur compound, organic nitrogen for generating foul gas etc., substantially improve the ring in place
Border.
It belongs to bacterium, Bacillaceae.
It belongs to bacillus.
The invention also discloses a kind of wheat planting microorganism preparation methods, comprise the following steps：
S1, will be heat-treated 6 minutes through 80 DEG C of water-baths after strain normal saline dilution；
S2, it is coated on plating medium, the constituent (g/L) of culture medium：Peptone 7, sodium chloride 9, beef
Cream 4, pH value 7.1；
S3, condition of culture：33 DEG C, 155r/min of temperature cultivates 26h；
S4, the fermentation medium that the culture solution Jing Guo previous step is transferred to fermentation tank in 32 DEG C of temperature, stir 155r/min
Under the conditions of ferment；
Fermentation medium components (g/L)：Peptone 19, yeast extract 8, glucose 19, disodium hydrogen phosphate 4, biphosphate
Potassium 4, manganese sulfate 0.5, calcium chloride 0.3.
Embodiment 3
A kind of wheat planting can form the poly- Vetsin of the superfine natural material of intensity with microorganism, be soil
Protective film, prevent fertile part and moisture loss；While it is proliferated, the amylase of high activity can be disengaged, hardly possible is decomposed big
Molecular substance resolves into available small-molecule substance.
It can synthesize the substances such as a variety of organic acids, enzyme, physiological activity and other a variety of nutrients being easily utilized.
Its is antibacterial, harmful power of going out is strong：With the advantage of taking up space, inhibit the growth of the harmful microbes such as harmful bacteria, pathogen
Breeding
It can decompose organic substance, organic sulfur compound, organic nitrogen for generating foul gas etc., substantially improve the ring in place
Border.
It belongs to bacterium, Bacillaceae.
It belongs to bacillus.
The invention also discloses a kind of wheat planting microorganism preparation methods, comprise the following steps：
S1, will be heat-treated 10 minutes through 80 DEG C of water-baths after strain normal saline dilution；
S2, it is coated on plating medium, the constituent (g/L) of culture medium：Peptone 10, sodium chloride 10, ox
Meat extract 4, pH value 7.3；
S3, condition of culture：34 DEG C, 170r/min of temperature cultivates 26h；
S4, the fermentation medium that the culture solution Jing Guo previous step is transferred to fermentation tank in 35 DEG C of temperature, stir 160r/min
Under the conditions of ferment；
Fermentation medium components (g/L)：Peptone 19, yeast extract 10, glucose 20, disodium hydrogen phosphate 4, biphosphate
Potassium 4, manganese sulfate 0.5, calcium chloride 0.4.
Embodiment 4
A kind of wheat planting can form the poly- Vetsin of the superfine natural material of intensity with microorganism, be soil
Protective film, prevent fertile part and moisture loss；While it is proliferated, the amylase of high activity can be disengaged, hardly possible is decomposed big
Molecular substance resolves into available small-molecule substance.
It can synthesize the substances such as a variety of organic acids, enzyme, physiological activity and other a variety of nutrients being easily utilized.
Its is antibacterial, harmful power of going out is strong：With the advantage of taking up space, inhibit the growth of the harmful microbes such as harmful bacteria, pathogen
Breeding
It can decompose organic substance, organic sulfur compound, organic nitrogen for generating foul gas etc., substantially improve the ring in place
Border.
It belongs to bacterium, Bacillaceae.
It belongs to bacillus.
The invention also discloses a kind of wheat planting microorganism preparation methods, comprise the following steps：
S1, will be heat-treated 20 minutes through 80 DEG C of water-baths after strain normal saline dilution；
S2, it is coated on plating medium, the constituent (g/L) of culture medium：Peptone 12, sodium chloride 12, ox
Meat extract 5, pH value 7.5；
S3, condition of culture：35 DEG C, 180r/min of temperature cultivates 26h；
S4, the fermentation medium that the culture solution Jing Guo previous step is transferred to fermentation tank in 37 DEG C of temperature, stir 200r/min
Under the conditions of ferment；
Fermentation medium components (g/L)：Peptone 120, yeast extract 12, glucose 22, disodium hydrogen phosphate 5, di(2-ethylhexyl)phosphate
Hydrogen potassium 5, manganese sulfate 0.6, calcium chloride 0.5.
7g peptones, 10g sodium chloride, the mixing of 4g beef extracts are added in 1L distilled water, then gone out in 125 DEG C of high pressures in S2
Bacterium obtains culture medium.
The foregoing is only a preferred embodiment of the present invention, but protection scope of the present invention be not limited thereto,
Any one skilled in the art in the technical scope disclosed by the present invention, technique according to the invention scheme and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (8)
1. a kind of wheat planting is with microorganism, which is characterized in that it can form the poly- bran amine of the superfine natural material of intensity
Acid is the protective film of soil, prevents fertile part and moisture loss；While it is proliferated, the amylase of high activity can be disengaged, by difficulty
The macromolecular substances of decomposition resolve into available small-molecule substance.
2. a kind of wheat planting according to claim 1 is with microorganism, which is characterized in that it can synthesize a variety of organic
The substances such as acid, enzyme, physiological activity and other a variety of nutrients being easily utilized.
3. a kind of wheat planting according to claim 1 is with microorganism, which is characterized in that its is antibacterial, harmful power of going out is strong：Have
Take up space advantage, inhibits the harmful microbes growth and breedings such as harmful bacteria, pathogen.
4. a kind of wheat planting according to claim 1 is with microorganism, which is characterized in that it can decompose generation effluvium
Organic substance, organic sulfur compound, organic nitrogen of body etc. substantially improve the environment in place.
5. a kind of wheat planting according to claim 1 is with microorganism, which is characterized in that it belongs to bacterium, bacillus
Section.
6. a kind of wheat planting according to claim 1 is with microorganism, which is characterized in that it belongs to bacillus.
7. for cultivating a kind of wheat planting microorganism preparation method described in claim 1, which is characterized in that including with
Lower step：
S1, will be heat-treated 5-20 minutes through 80 DEG C of water-baths after strain normal saline dilution；
S2, it is coated on plating medium, the constituent (g/L) of culture medium：Peptone 6-12, sodium chloride 8-12, ox
Meat extract 3-5, pH value 7.0-7.5；
S3, condition of culture：32-35 DEG C, 150-180r/min of temperature cultivates 26h；
S4, the fermentation medium that the culture solution Jing Guo previous step is transferred to fermentation tank in 30-37 DEG C of temperature, stir 150-200r/
It ferments under the conditions of min；
Fermentation medium components (g/L)：Peptone 18-20, yeast extract 7-12, glucose 18-22, disodium hydrogen phosphate 3-5, phosphorus
Acid dihydride potassium 3-5, manganese sulfate 0.4-0.6, calcium chloride 0.2-0.5.
8. a kind of wheat planting microorganism preparation method according to claim 1, which is characterized in that by 7g eggs in S2
White peptone, 10g sodium chloride, the mixing of 4g beef extracts add in 1L distilled water, then in 125 DEG C of high pressure sterilizations, obtain culture medium.
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| CN201810040031.8A CN108102968A (en) | 2018-01-16 | 2018-01-16 | A kind of wheat planting is with microorganism and preparation method thereof |
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Citations (6)
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| CN101948769A (en) * | 2010-08-20 | 2011-01-19 | 南京农业大学 | Bacteria YXY-C1 for preparing gamma-polyglutamic acid by solid fermentation and products thereof |
| CN105420169A (en) * | 2015-12-31 | 2016-03-23 | 中国科学院成都生物研究所 | Gamma-polyglutamic acid producing strain and method for producing gamma-polyglutamic acid organic fertilizer |
| CN105602863A (en) * | 2015-12-14 | 2016-05-25 | 南京工业大学 | Bacillus subtilis strain for high yield of lipopeptide antibiotic and poly-gamma-glutamic acid |
| JP2017093321A (en) * | 2015-11-19 | 2017-06-01 | 花王株式会社 | Bacillus subtilis mutants and uses thereof |
| KR20170090878A (en) * | 2016-01-29 | 2017-08-08 | 영남대학교 산학협력단 | Novel Bacillus sp. FBL-2 Strain or Method for Preparing of Poly(γ-glutamic acid) |
| CN107118990A (en) * | 2017-05-22 | 2017-09-01 | 中国科学院成都生物研究所 | Its production γ PGA of high yield γ PGA bacterial strains and application method |
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2018
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Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101948769A (en) * | 2010-08-20 | 2011-01-19 | 南京农业大学 | Bacteria YXY-C1 for preparing gamma-polyglutamic acid by solid fermentation and products thereof |
| JP2017093321A (en) * | 2015-11-19 | 2017-06-01 | 花王株式会社 | Bacillus subtilis mutants and uses thereof |
| CN105602863A (en) * | 2015-12-14 | 2016-05-25 | 南京工业大学 | Bacillus subtilis strain for high yield of lipopeptide antibiotic and poly-gamma-glutamic acid |
| CN105420169A (en) * | 2015-12-31 | 2016-03-23 | 中国科学院成都生物研究所 | Gamma-polyglutamic acid producing strain and method for producing gamma-polyglutamic acid organic fertilizer |
| KR20170090878A (en) * | 2016-01-29 | 2017-08-08 | 영남대학교 산학협력단 | Novel Bacillus sp. FBL-2 Strain or Method for Preparing of Poly(γ-glutamic acid) |
| CN107118990A (en) * | 2017-05-22 | 2017-09-01 | 中国科学院成都生物研究所 | Its production γ PGA of high yield γ PGA bacterial strains and application method |
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| 何若天等: "《农用益生菌生产与应用手册》", 29 February 2016, 金盾出版社 * |
| 揭益寿等: "《中国农业发展模式创新与农业现代化（第二版）》", 31 January 2013, 中国矿业大学出版社 * |
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