CN108088822A - A kind of chip type light derivatization device of aflatoxin and sulfa drugs - Google Patents
A kind of chip type light derivatization device of aflatoxin and sulfa drugs Download PDFInfo
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- CN108088822A CN108088822A CN201611038988.6A CN201611038988A CN108088822A CN 108088822 A CN108088822 A CN 108088822A CN 201611038988 A CN201611038988 A CN 201611038988A CN 108088822 A CN108088822 A CN 108088822A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/74—Optical detectors
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Abstract
The chip type light derivatization device of a kind of aflatoxin of the present invention and sulfa drugs, excitation light source is used as using UV LED (LED) of the centre wavelength between 280 380nm, using the quartz of high transparency, holvi glass, PDMS or fluorinated ethylene propylene copolymer (FEP) are used as chip substrate, 10~500 microns of width is produced on chip substrate, 10~260 microns of depth, the rectangle or the trapezoidal tank circuit that 5 500 millimeters of length are as derivatization reaction pond, the tank circuit is linear or pectination or sine wave shape arrangement, derivative pool volume is 75 μ L of 5nL, 1/13 to ten a ten thousandths of only traditional derivatization device pond body product.The chip type light derivatization device of the present invention makes the fluorescence intensity of aflatoxin B1 improve 6.5 times, suitable for light derivative and Micro flow injection analysis/small sized flow Injection Analysis after the column of chip capillary cataphoresis, chip microfluidics analysis, capillary liquid chromatography, micro column liquid chromatography, ultrahigh pressure liquid phase chromatography and conventional liquid phase chromatography.
Description
Technical field
Patent of the present invention is related to micro-fluidic chemical analysis, capillary high performance liquid chromatography (μ-HPLC), micro-flowing injection point
The field of optical detection of (μ-FIA) is analysed, is derived more specifically to a kind of for the light of aflatoxin and sulfa drugs
Change device.
Background technology
Light derivatization device is widely used in high performance liquid chromatography (HPLC)-fluoroscopic examination standard measure detection aflatoxin and sulphur
Amine drug, as standard GB/T/T 18979-2003 and GB/T 23212-2008 and Chinese Pharmacopoeia in 2015 spread out light
Biochemical device is added in one chapter of aflatoxin measuring method, for the trace aspergillus flavus in auxiliary measuring medicinal material, medicine materical crude slice and preparation
Toxin.With the development of the times, scientific and technological progress, national standard and European Union's examination criteria are to aflatoxin in all kinds of substances
Limited Doses continuously decrease, and the difficulty for analyzing detection is continuously improved, and Monitoring lower-cut, must already below the level of conventional fluorescent detector
Detection sensitivity must be improved by derivatization or molecular marking technique.The Green Chemistry for developing and being rooted in the hearts of the people in recent ten years
Concept and technology, target are fundamentally to solve the problems, such as that environmental pollution and lab assistant are healthy and safe.Correspondingly, analyzing
Chemical field, it is current development side that development reagent dosage reduces by 90% or even 99% analytical equipment and technology than existing equipment
To.Therefore microfluidic analysis technology is also come into being.
But the pond body product of the analytical instrument of micrometeor requirement detector also must be small, such as chip microfluidics point
Analysis and micro-flowing injection analysis require detection cell volume in 2.5nL~70nL, capillary high performance liquid chromatography requirement detection pond body
For product in 20nL~500nL, micro column liquid chromatography requires detection cell volume in the μ L of 0.5 μ L~2.It is detected from analysis, derivatization
The pond body product of device is the smaller the better；But pond body product is smaller, the amount of light radiation is lower, and the derivatization reaction time is shorter, can cause to spread out
The rate that comes into force reduces.It is to exchange high spread out for chromatographic column separative efficiency and permission chromatography peak stretching is sacrificed in conventional analysis at present
Come into force rate, mating for the fluorescence detector of pond body product only 12 μ L for example with the light derivatization device of derivative pond body product 1mL, derives pond
Volume is 83 times of detector cell volume！But ultrahigh pressure liquid phase chromatography (UPLC) has been arrived, the requirement to dead volume outside column is very high,
The derivatization device and the sum of detector, the two that so big pond body is accumulated cannot be used no more than 10 μ L.At present, pond body product is
Several microlitres and the efficient light derivatization device of derivatization have not been reported.For microfluidic analysis and capillary liquid chromatography,
The pond body product of post column derivatization device can only be≤detection cell volume, otherwise can influence separative efficiency.
But aflatoxin light derivatization device used so far, whether (Fan Haidong, Wang Lingfeng, the Zhao Dong of scientific research
Lucky " aflatoxin B1 in self-control photochemical derivatization reactor-Soybean by High Performance Liquid Chromatography oil ", physical and chemical inspection-change
Credit volume, 2013,49,999-1001) or (http of commercialization://www.josvok.com/product_show.php
Id=57) (http://www.18show.cn/zt410303/Product_10226818.html), derivatization pond is all adopted
It is woven with FEP (fluorinated ethylene propylene copolymer) pipe of high transparency, pond body product is 100~1000 μ L, and light source is all using ripple
A length of 254nm low pressure mercury lamps, when the service life is 2000~3000 small.For chip capillary cataphoresis, chip microfluidics analysis, it is miniature
Flow Injection Analysis, capillary liquid chromatography, micro column liquid chromatography, ultrahigh pressure liquid phase chromatography column after light derive, at present also not
See nanoliter the report of the light derivatization device accumulated to micro updating pond body.
The content of the invention
In view of the above-mentioned problems, in order to improve the deficiencies in the prior art, based on to the further of photochemical derivatization reaction mechanism
Further investigation, the present invention propose that centre wavelength is used by the ultraviolet LED light source of 280~380nm traditional low pressure mercury lamp to be replaced to make
For excitation light source；The light derivatization reaction passage arranged using linear on chip or square-wave-shaped or sine wave shape replaces traditional
The derivatization reaction pipe of netted braiding structure, reaction channel are 10~500 μm of width, 10~260 μm of depth, length 5-500mm
Rectangle or the trapezoidal tank circuit, derivative pond body product is only 5nL-75 μ L；The experiment proved that 7.5 μ L pond bodies product light using the present invention spreads out
Biochemical device can make the fluorescence intensity of aflatoxin G 1 improve 7 times, and the fluorescence intensity of aflatoxin B1 is made to improve 6 times, with
Traditional 1000 μ L pond bodies product light derivatization device similar efficiency.
The technical scheme is that：
The chip type light derivatization device of a kind of aflatoxin and sulfa drugs is derived anti-by ultraviolet LED light source, chip
Answer passage, chip substrate, reflector, LED substrate and groups of fins into, it is characterised in that：
One or two or more the ultraviolet LED light source arranged along straight line is located at the top of chip derivatization reaction passage as light
The excitation light source of derivatization reaction；The ultraviolet LED light source of one or two or more is welded on the lower surface of LED substrate；Ultraviolet LED light
Source is located at the top of derivatization reaction pipe, and lower surface and the plane where chip derivatization reaction passage rectangular region of LED substrate are put down
Row；Straight line residing for one or two or more the ultraviolet LED light source along straight line arrangement and the chip derivatization reaction in rectangular region
Passage is parallel and ultraviolet LED light source residing for straight line plane projection where rectangular region it is long by chip derivatization reaction passage
The center of square region；Certain distance is equipped between ultraviolet LED light source and chip derivatization reaction passage；
Chip derivatization reaction passage is arranged on chip substrate, for rectangle or the trapezoidal tank circuit, linear, square-wave-shaped or just profound
Ripple shaped formation；The material of chip substrate is the quartz of high transparency, (perfluoroethylenepropylene is copolymerized by holvi glass, PDMS or FEP
Object)；
Reflector is the structure that a upper surface is plane, and reflector is located at the lower section of chip derivatization reaction passage, reflector
Upper surface is parallel with plane residing for chip derivatization reaction passage rectangular region, and between reflector and chip derivatization reaction passage
Equipped with certain distance；Cooling fin is close to heat dissipation of the LED substrate upper surface for ultraviolet LED light source.
The centre wavelength of the ultraviolet LED light source is between 280-380nm, 60-140 ° of the angle of divergence；The ultraviolet LED light
Refer to equipped with certain distance leave gap between them between source and chip derivatization reaction passage, make ultraviolet LED light source equably
It is radiated on chip derivatization reaction passage；The distance of ultraviolet LED light source and chip derivatization reaction passage is 0.1-7 millimeters.
Wide 10-500 microns of the chip derivatization reaction passage, 10-260 microns of depth, 5-500 millimeters long, internal volume 5 receive
Rise -75 microlitres；Refer to equipped with certain distance leave gap between them between reflector and chip derivatization reaction passage, for carrying
High reflector efficiency；Distance 0.1-7 millimeters between chip derivatization reaction passage and reflector.
The material of the reflector is polishing stainless steel plate or polishing aluminium sheet, reflector while and heat sinking function.
The LED substrate and the material of cooling fin are the good metal material of heat conduction, such as aluminium or copper, and LED substrate and are dissipated
Backing fits closely, beneficial to heat dissipation.
The ultraviolet LED light source of one or two or more has sustained height after being welded in LED substrate and welding, i.e., ultraviolet
LED light source lower end is in same level.
Compared with prior art, Photochemical derivatization device of the invention has the following advantages that：
1. traditional light derivatization device is using Weaving type derivative reaction pipe, pond body product is between 100~1000 μ L, it is impossible to should
For the post column derivatization in microfluidic analysis and capillary liquid chromatography analysis；And the present invention using fine inner diameter linear or
Pectination or sine wave shape arrangement derivative reaction pipe, reaction tank volume only 5nL-75 μ L, not only pond body product with micro-fluidic point
Analysis system matches, and light derivatization efficiency is greatly improved and reduces cost；
2. traditional light derivatization device uses 254nm mercury lamp ultraviolet sources, and the present invention uses 280-380nm centre wavelengths
LED ultraviolet sources, still obtain very high derivatization efficiency.The LED of 380nm wavelength, service life are arrived for centre wavelength 355
More than when 20000 is small, ultraviolet LED light source total power consumption only 1-3W, either service life, stability, power consumption, volume or even cost refer to
Mark, ultraviolet LED light source are all far superior to traditional low pressure mercury lamp.
3. the derivative pond body product only 5nL-75 μ L of chip type light derivatization device, make chip-scale microfluidic analysis and capillary
The post-column derivation of liquid-phase chromatographic analysis is achieved.It is micro- that the light derivatization device of the present invention is suitable for chip capillary cataphoresis, chip
Flow control analysis, capillary liquid chromatography, micro column liquid chromatography, ultrahigh pressure liquid phase chromatography and conventional liquid phase chromatography column after light derive
With Micro flow injection analysis/small sized flow Injection Analysis, detection sensitivity is improved.
4th, chip Photochemical derivatization device pond body accumulates very small, light and derives efficient, at low cost, compact, effectively improves
The detection sensitivity of detection aflatoxin and sulfa drugs, practicability are very strong.
Description of the drawings
Fig. 1 (a) and Fig. 1 (b) is two views of chip type light derivatization device, wherein：(a) it is vertical direction sectional view,
(b) it is the top view of chip derivatization reaction passage.In figure：1- ultraviolet LED light sources, 2- chip derivatization reaction passages, 3- chip bases
Plate, 4- reflectors, 5-LED substrates and 6- cooling fins.
Fig. 2 is the detection spectrogram of embodiment 1.
Specific embodiment
The chip type light derivatization device of a kind of aflatoxin and sulfa drugs is derived by ultraviolet LED light source 1, chip
Reaction channel 2, chip substrate 3, reflector 4, LED substrate 5 and cooling fin 6 form, it is characterised in that：
One or two or more the ultraviolet LED light source 1 arranged along straight line is located at the top conduct of chip derivatization reaction passage 2
The excitation light source of light derivatization reaction；The ultraviolet LED light source 1 of one or two or more is welded on the lower surface of LED substrate 5；It is ultraviolet
LED light source 1 is located at the top of derivatization reaction pipe 2, lower surface and the 2 rectangular region institute of chip derivatization reaction passage of LED substrate 5
It is parallel in plane；Straight line residing for one or two or more the ultraviolet LED light source 1 along straight line arrangement and the chip in rectangular region
Derivatization reaction passage 2 is parallel and ultraviolet LED light source 1 residing for straight line plane projection where rectangular region derived by chip
The center of 2 rectangular region of reaction channel；Certain distance is equipped between ultraviolet LED light source 1 and chip derivatization reaction passage 2；
Chip derivatization reaction passage 2 is arranged on chip substrate 3, is rectangle or the trapezoidal tank circuit, linear, square-wave-shaped or just
Profound ripple shaped formation；The material of chip substrate 3 is quartz, holvi glass, PDMS or the FEP (perfluoroethylenepropylenes of high transparency
Copolymer)；
Reflector 4 is the structure that a upper surface is plane, and reflector 4 is located at the lower section of chip derivatization reaction passage 2, reflective
4 upper surface of plate is parallel with plane residing for 2 rectangular region of chip derivatization reaction passage, and reflector 4 leads to chip derivatization reaction
Certain distance is equipped between road 2；Cooling fin 6 is close to heat dissipation of 5 upper surface of LED substrate for ultraviolet LED light source.
The centre wavelength of ultraviolet LED light source 1 is between 280-380nm, 60-140 ° of the angle of divergence；Ultraviolet LED light source 1 and core
Refer to equipped with certain distance leave gap between them between piece derivatization reaction passage 2, ultraviolet LED light source 1 is made equably to irradiate
On chip derivatization reaction passage 2；The distance of ultraviolet LED light source 1 and chip derivatization reaction passage 2 is 0.1-7 millimeters.
Wide 10-500 microns of chip derivatization reaction passage 2,10-260 microns of depth, 5-500 millimeters long, 5 nanoliters of internal volume-
75 microlitres；Refer to equipped with certain distance leave gap between them between reflector 4 and chip derivatization reaction passage 2, for carrying
High reflector efficiency；0.1-7 millimeters of distance between chip derivatization reaction passage 2 and reflector 4.
The material of reflector 4 is polishing stainless steel plate or polishing aluminium sheet, reflector while and heat sinking function.
LED substrate 5 and the material of cooling fin 6 are the good metal material of heat conduction, such as aluminium or copper, and LED substrate 5 and heat dissipation
Piece 6 fits closely, beneficial to heat dissipation.
The ultraviolet LED light source 1 of one or two or more has sustained height after being welded in LED substrate 5 and welding, i.e., purple
Outer 1 lower end of LED light source is in same level.
Embodiment 1
Light derivatization device after a kind of ultra performance liquid chromatography (UPLC) column, excitation light source are 2 high-power centre wavelengths
365nm ultraviolet LEDs, 120 ° of the angle of divergence, rated current 500mA；The distance 3mm of ultraviolet LED light source and chip derivatization reaction passage；
Chip substrate material is the quartz glass of high transparency, which is provided with the chip derivatization reaction passage of pectination arrangement, and passage is wide by 150
μm, the rectangle tank circuit of 200 μm of depth, length 250mm, derivative pond body accumulates 7.5 μ L；Chip derivatization reaction passage and reflector distance
3mm；Reflector is stainless steel polishing plate；LED substrate and the material of cooling fin are metallic aluminium.By the chip type light derivatization device with
Its performance is evaluated in Waters e2475FLD types fluorescence detector (2 μ L pond bodies product) connection.Liquid phase chromatogram condition is：Chromatographic column：
C18 columns, 100mm × 2.1mm × 1.7 μm；Mobile phase A methanol：Mobile phase B pure water=45:55；Flow rate of mobile phase：0.3mL/
min；Sample introduction：10 μ L 0.6ppb aflatoxin mix mark.
Experimental result：
Fig. 2 is detection spectrogram：(1) direct injected without derivative after chromatographic isolation, is detected, to Huang with Waters e2475FLD
Aspertoxin B1 Monitoring lower-cuts are 0.14ppb；(2) use chip type light derivatization device of the present invention, be connected on column outlet and
Between detector entrance, 6.5 times are improved to aflatoxin B1 detection sensitivity, detection is limited to 0.02ppb.
Embodiment 2
Chip type light derivatization device as described in Example 1, by itself and the combination of Waters e2475FLD moneys fluorescence detector
Analysis 4 kinds of sulfa drugs of detection.Excitation wavelength 230nm, launch wavelength 400nm.
Experimental result：Sample introduction 5ppb sulphadiazines (SDZ), Waters e2475FLD are not detected when not coupling derivatization device；
After connection chip type light derivatization device of the present invention, 5ppb sulphadiazines (SDZ) can detect, detection signal-to-noise ratio 15.
Design philosophy according to the embodiment of the present invention modifies to the specific embodiment and application range of embodiment pair
It is obvious for those skilled in the art.The present invention is not intended to be limited to embodiment illustrated herein, and
It is to fit to the most wide scope consistent with General Principle disclosed herein and features of novelty.It is all to think according to present invention design
Want the change of any no creative work made all within protection scope of the present invention.
Claims (6)
1. the chip type light derivatization device of a kind of aflatoxin and sulfa drugs is derived by ultraviolet LED light source (1), chip
Reaction channel (2), chip substrate (3), reflector (4), LED substrate (5) and cooling fin (6) composition, it is characterised in that：
One or two or more the ultraviolet LED light source (1) arranged along straight line is located at the top conduct of chip derivatization reaction passage (2)
The excitation light source of light derivatization reaction；The ultraviolet LED light source (1) of one or two or more is welded on the lower surface of LED substrate (5)；
Ultraviolet LED light source (1) is located at the top of derivatization reaction pipe (2), lower surface and the chip derivatization reaction passage (2) of LED substrate (5)
Plane where rectangular region is parallel；One or two or more along straight line arrange ultraviolet LED light source (1) residing for straight line with it is rectangular
Chip derivatization reaction passage (2) in shape region is parallel and ultraviolet LED light source (1) residing for straight line it is flat where rectangular region
Face is projected through the center of chip derivatization reaction passage (2) rectangular region；Ultraviolet LED light source (1) leads to chip derivatization reaction
Certain distance is equipped between road (2)；
Chip derivatization reaction passage (2) is arranged on chip substrate (3), for rectangle or the trapezoidal tank circuit (groove), linear, side
The arrangement of wavy or sine wave shape；The material of chip substrate (3) is the quartz of high transparency, holvi glass, PDMS or FEP are (complete
Fluoroethylene-propylene copolymer)；
Reflector (4) is the structure that a upper surface is plane, and reflector (4) is located at the lower section of chip derivatization reaction passage (2), instead
Tabula rasa (4) upper surface is parallel with plane residing for chip derivatization reaction passage (2) rectangular region, and reflector (4) spreads out with chip
Certain distance is equipped between raw reaction channel (2)；Cooling fin (6) is close to LED substrate (5) upper surface for ultraviolet LED light source
Heat dissipation.
2. chip type light derivatization device according to claim 1, it is characterised in that：The center of the ultraviolet LED light source (1)
Wavelength is between 280-380nm, 60-140 ° of the angle of divergence；Between the ultraviolet LED light source (1) and chip derivatization reaction passage (2)
Refer to leave gap between them equipped with certain distance, ultraviolet LED light source (1) is made equably to be radiated at chip derivatization reaction passage
(2) on；The distance of ultraviolet LED light source (1) and chip derivatization reaction passage (2) is 0.1-7 millimeters.
3. chip type light derivatization device according to claim 1, it is characterised in that：The chip derivatization reaction passage (2)
Wide 10-500 microns, 10-260 microns of depth, 5-500 millimeters long, 5 nanoliters -75 microlitres of internal volume；Reflector (4) spreads out with chip
Refer to leave gap between them equipped with certain distance between raw reaction channel (2), for improving reflector efficiency；Chip derives anti-
Answer 0.1-7 millimeters of distance between passage (2) and reflector (4).
4. chip type light derivatization device according to claim 1, it is characterised in that：The material of the reflector (4) is throwing
Light stainless steel plate or polishing aluminium sheet, reflector while and heat sinking function.
5. chip type light derivatization device according to claim 1, it is characterised in that：The LED substrate (5) and cooling fin
(6) material is the good metal material of heat conduction, and such as aluminium or copper, and LED substrate (5) and cooling fin (6) fit closely, beneficial to scattered
Heat.
6. chip type light derivatization device according to claim 1, it is characterised in that：The ultraviolet LED of one or two or more
Light source (1) has sustained height after being welded in LED substrate (5) and welding, i.e. ultraviolet LED light source (1) lower end is in same water
In plane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611038988.6A CN108088822B (en) | 2016-11-21 | 2016-11-21 | Chip type light derivatization device for aflatoxin and sulfanilamide drugs |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611038988.6A CN108088822B (en) | 2016-11-21 | 2016-11-21 | Chip type light derivatization device for aflatoxin and sulfanilamide drugs |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108088822A true CN108088822A (en) | 2018-05-29 |
| CN108088822B CN108088822B (en) | 2020-05-26 |
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ID=62170161
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611038988.6A Active CN108088822B (en) | 2016-11-21 | 2016-11-21 | Chip type light derivatization device for aflatoxin and sulfanilamide drugs |
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| CN (1) | CN108088822B (en) |
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| CN108931511A (en) * | 2018-06-04 | 2018-12-04 | 常州市武进区半导体照明应用技术研究院 | The fluorescence detection device and method of ultraviolet LED |
| CN109358128A (en) * | 2018-12-03 | 2019-02-19 | 南京大学 | A kind of organic nitrogen-organic carbon tandem online test method and device |
| CN109406705A (en) * | 2018-11-29 | 2019-03-01 | 南京大学 | A kind of liquid chromatogram combination type organic carbon detector and its application method |
| CN109991346A (en) * | 2019-04-18 | 2019-07-09 | 南京大学 | A kind of micro-fluidic Ultraviolet Oxidation device for organic nitrogen analysis |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108931511A (en) * | 2018-06-04 | 2018-12-04 | 常州市武进区半导体照明应用技术研究院 | The fluorescence detection device and method of ultraviolet LED |
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| CN109406705A (en) * | 2018-11-29 | 2019-03-01 | 南京大学 | A kind of liquid chromatogram combination type organic carbon detector and its application method |
| CN109406705B (en) * | 2018-11-29 | 2024-04-09 | 南京大学 | Liquid chromatography combined organic carbon detector and application method thereof |
| CN109358128A (en) * | 2018-12-03 | 2019-02-19 | 南京大学 | A kind of organic nitrogen-organic carbon tandem online test method and device |
| CN109358128B (en) * | 2018-12-03 | 2024-04-09 | 南京大学 | Organic nitrogen-organic carbon serial on-line detection method and device |
| CN109991346A (en) * | 2019-04-18 | 2019-07-09 | 南京大学 | A kind of micro-fluidic Ultraviolet Oxidation device for organic nitrogen analysis |
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| Publication number | Publication date |
|---|---|
| CN108088822B (en) | 2020-05-26 |
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