CN108085237A - The PCR pipe lid and application method of a kind of freeze-drying - Google Patents
The PCR pipe lid and application method of a kind of freeze-drying Download PDFInfo
- Publication number
- CN108085237A CN108085237A CN201711165299.6A CN201711165299A CN108085237A CN 108085237 A CN108085237 A CN 108085237A CN 201711165299 A CN201711165299 A CN 201711165299A CN 108085237 A CN108085237 A CN 108085237A
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- CN
- China
- Prior art keywords
- pcr
- freeze
- cover
- drying
- test tube
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5021—Test tubes specially adapted for centrifugation purposes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/041—Connecting closures to device or container
- B01L2300/042—Caps; Plugs
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention discloses the PCR pipe lids and application method of a kind of freeze-drying.The test tube cap includes capping and cover, is made of transparent plastic, is an entirety, can form a kind of multiple-connecting-pipe lid.The cover is extended type, is provided with seal land, supports rib and symmetrical two windows.In freezing dry process, pipe is placed on nozzle, and lid is extremely supported at rib, exposes the first half of window, and moisture is evaporated from the window of exposing.After the completion of drying, freeze drier laminate presses down, and pipe lid is pressed into pipe, pipe is covered tightly.Seal land ensures the sealing of test tube.The present invention is suitable for 96 orifice plate of PCR pipe, 8 unions of PCR or PCR of capping 0.1mL or 0.2mL, avoids the detection reagent after freeze-drying and contacts and make moist with air, is conducive to the preservation of reagent.
Description
Technical field
Test tube cap and application method more particularly to one kind the present invention relates to a kind of freeze-drying is cold for detection reagent
Freeze desiccant PCR pipe lid and application method.
Background technology
Quantitative fluorescent PCR is the major technique of clinical molecular detection, than traditional ELISA the methods of is sensitiveer, it is more special,
It is quantitative it is more accurate, window phase is shorter, more rapidly.But the typically liquid reagent that this method uses at present, it is necessary to using cold chain
Transport and storage, packaging, logistics, storage cost are high, can also expand because of cold chain transportation failure, liquid relief error, multigelation, gas dissolubility
Increase the loss that the reasons such as product pollution cause up to 25%, and using when take out to thaw from refrigerator and need waiting in 30-40 minute,
Operating process is also sufficiently complex, is easy to cause error.Therefore the technology use cost is too high, limits its application, at present only
In-vitro diagnosis about 10% is accounted for, is mainly used in Grade III Class A hospital.
Therefore, during fluorescence quantitative PCR detection reagent is promoted, one kind is advantageous alternative is that be made under room temperature
Stable drying type reagent.Due to the main component in reagent, such as Taq enzyme, dNTP, primer, probe, freezing must be maintained at
Under state, the drying of this kind of reagent must be using the method for vacuum freeze drying.In this process, reagent freezes by precooling
Cheng Bing, in freeze drier, moisture directly distil from solid-state be absorbed in condenser to achieve the purpose that for gaseous state it is dry.
Dried reagent generally reaches high aridity（Moisture<3%）, it is necessary to it avoids and air（Relative humidity 60-100%）
Contact influences the shelf-life in order to avoid the moisture absorption is melted.
It is a kind of advantageous alternative is that liquid reagent is divided in the test tube that a kind of user can directly use on the machine（Such as PCR
Pipe or 96 orifice plates）In, then be freeze-dried.After drying, dry inert gas such as nitrogen is full of in drying instrument, is beaten
Vacuum breaker, then the hydraulic pressure mechanism using drying instrument and laminate moving up and down, test tube is covered tightly, then opens drying box.This
Sample reagent is the independent packaging of single part, and what can be brought to avoid foregoing liquid relief error, multigelation, Aerosol Pollution etc. is latent
It is being lost.But currently without the test tube cap being freeze-dried suitable for PCR pipe, 8 unions of PCR or 96 orifice plates of PCR.
The content of the invention
The present invention provides a kind of test tube cap and its application method, the test tube cap are used directly in 0.2 mL or 0.1 mL
96 orifice plate of PCR pipe, 8 unions of PCR or PCR in be detected the freeze-drying of reagent, convenient for directly in freeze drier
Capping is completed, is conducive to the preservation of dried reagent.
The technical solution adopted by the present invention to solve the technical problems is：The test tube cap of the present invention includes capping（1）And add
Long cover（2）.Capping and cover are an entirety, are made of transparent plastics.Preferably, the test tube cap be suitable for directly into
Row PCR reacts.Further, the transparency of capping is suitable for directly carrying out Fluorescence PCR.Seal land is set in cover
（3）, support rib（4）And two symmetrical windows（5）, when pipe is placed on PCR pipe nozzle, pipe lid, which rests on, to be supported on rib,
Expose the first half of window.The window evaporation that moisture in drying process middle pipe can expose from cover, after the completion of dry, utilizes
The hydraulic pressure mechanism of freeze drier and laminate moving up and down, test tube is covered tightly, and seal land ensures the leakproofness of test tube, avoids
Reagent makes moist.
Further, test tube cap of the present invention could be provided as what is linked together by multiple identical test tube caps
Complex, i.e. multiple-connecting-pipe lid.Particularly, the 8 union lids that 8 pipe lids are connected as one be could be provided as.
The invention has the advantages that in freezing dry process, the moisture of detection reagent can be steamed from the window of pipe lid
Hair, the capping of test tube can be completed directly in freeze drier, dry reagent is avoided to be connect with air after the completion of freeze-drying
It touches and makes moist.
Description of the drawings
The invention will be further described with reference to the accompanying drawings and examples.
Fig. 1 is the structure diagram of the present invention.1 is capping in figure, and 2 be cover, and 3 be seal land, and 4 be to support rib, and 5 be window
Mouthful.
Fig. 2 is test tube cap state in freezing dry process.
Fig. 3 is test tube cap state after the completion of freeze-drying.
Fig. 4 is the 8 union lids of the present invention.
Fig. 5 is seal land and the permanent section structure for supporting rib.1 is that seal land/support rib 2 is radius in figure, and 3 be cover.
Specific embodiment
In Fig. 1, capping（1）And cover（2）For an entirety, it is made of transparent plastic.Capping is smooth flat, is applicable in
In directly progress PCR reactions.Further, the transparency of capping is suitable for directly carrying out Fluorescence PCR.Cover is lengthening
Type is provided with seal land（3）With support rib（4）.Rib stay pipe lid in freeze-drying is supported to make window（5）It opens wide, water is provided
Divide the passage of evaporation, seal land ensures the sealing of test tube when Guan Gai is covered tightly.
On the PCR pipe nozzle equipped with reagent, the present invention is put, lid exposes the first half of window, Ran Houfang to rib is supported
Enter in freeze drier, in freezing dry process, moisture is evaporated from open window, dry using freezing after the completion of freeze-drying
Shifting function under the dry liftable laminate of instrument, covers tightly test tube in freeze drier, so as to fulfill the purpose of the present invention.
Can using identical or different material, different size of pipe lid be made, to adapt in the same proportion in the present invention
The container of different size and species, such as the PCR pipe of 0.1mL, the PCR pipe of 0.2mL, the centrifuge tube of 1.5mL, the centrifugation of 15mL
Pipe, centrifuge tube or cillin bottle of 50mL etc..
Those skilled in the art's simply change and replacement, all within protection scope of the present invention.
Claims (10)
1. the PCR pipe lid and application method of a kind of freeze-drying, it is characterised in that：The test tube cap includes capping and lid
Body is made of transparent plastic；The capping and cover are an entirety, and multiple-connecting-pipe lid can be combined into different number；It uses
The test tube cap is detected the freeze-drying of reagent, and capping is completed in freeze drier, avoids dry reagent and sky
Gas is contacted and made moist.
2. a kind of PCR pipe lid of freeze-drying according to claim 1, it is characterised in that：The capping is smooth
Plane, suitable for directly carrying out PCR reactions；Further, it is anti-to be suitable for directly progress fluorescent PCR for the transparency of the capping
It should.
3. a kind of PCR pipe lid of freeze-drying according to claim 1, it is characterised in that：The cover is circle,
Diameter is consistent with PCR test tube internal diameters, is usually 6.0mm；Longitudinal direction is extended type, and length 7.0-9.0mm is preferably 8.5mm.
4. extended type cover according to claim 3, it is characterised in that：Seal land is provided in the cover, is supported
Rib and two symmetrical windows；The seal land is loop configuration, and cross section is semicircle type, radius 0.4-0.6mm, preferably
For 0.5mm；It is preferably 3mm apart from capping 2.5-3.5mm, is in close proximity to the top edge of window.
5. cover according to claim 3, it is characterised in that：The support rib is semicircular configuration, starting and termination
In the edge of window, cross section is semicircle type, and radius is consistent with seal land, is preferably 3.0mm apart from seal land 2.5-3.5mm.
6. cover according to claim 3, it is characterised in that：Two symmetrical windows, length 5.0-
6.0mm is preferably 5.5mm, and width is the 1/5-1/3 of cover diameter, is preferably the 1/4 of cover diameter；Bottom is hatch frame.
7. a kind of PCR pipe lid of freeze-drying according to claim 1, it is characterised in that：The capping and cover
For an entirety, multiple-connecting-pipe lid can be combined into different number；Particularly, 8 union lids are combined by 8.
8. the PCR pipe lid and application method of a kind of freeze-drying according to claim 1, it is characterised in that：Described
Test tube cap is made of clear material, and can directly be carried out PCR or Fluorescence PCR, be sealed when being freeze-dried suitable for detection reagent
Cover 96 orifice plate of PCR pipe, 8 unions of PCR or PCR of 0.1mL or 0.2mL.
9. the PCR pipe lid and application method of a kind of freeze-drying according to claim 1, it is characterised in that：Described
Can using identical or different material, different size of pipe lid be made, to adapt to different size in the same proportion in test tube cap
With the PCR pipe of the container of species, such as 0.1mL, the PCR pipe of 0.2mL, the centrifuge tube of 1.5mL, the centrifuge tube of 15mL, 50mL's
Centrifuge tube or cillin bottle etc..
10. the PCR pipe lid and application method of a kind of freeze-drying according to claim 1, it is characterised in that：Described
Test tube cap is made of transparent plastic, it includes capping and cover, is an entirety, multiple can be combined into multiple-connecting-pipe lid；It is described
Cover for extended type, seal land is set, supports rib and two symmetrical windows；Before freeze-drying, pipe lid is placed on test tube
Mouthful, at lid to support rib, expose window；In freezing dry process, moisture is discharged from the window of exposing；After drying, freezing
Drying instrument laminate presses capping down, and cover is made to be covered tightly test tube, seal land ensures the sealing of test tube, keeps away completely into test tube
Exempt from dry reagent and air contact and make moist, be conducive to the preservation of reagent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711165299.6A CN108085237A (en) | 2017-11-21 | 2017-11-21 | The PCR pipe lid and application method of a kind of freeze-drying |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711165299.6A CN108085237A (en) | 2017-11-21 | 2017-11-21 | The PCR pipe lid and application method of a kind of freeze-drying |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108085237A true CN108085237A (en) | 2018-05-29 |
Family
ID=62172867
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711165299.6A Pending CN108085237A (en) | 2017-11-21 | 2017-11-21 | The PCR pipe lid and application method of a kind of freeze-drying |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108085237A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111974476A (en) * | 2020-09-11 | 2020-11-24 | 上海捷诺生物科技有限公司 | Freeze drying is with connecting test tube lid that stable leakproofness is good |
| WO2023072090A1 (en) * | 2021-10-25 | 2023-05-04 | 上海透景生命科技股份有限公司 | Polymerase chain reaction tube assembly |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140086881A1 (en) * | 2012-01-31 | 2014-03-27 | Kieu Hoang | Good healthy cells found in proteins, their applications, and process of making a medium to harvest the cells |
| CN203569075U (en) * | 2013-09-26 | 2014-04-30 | 钟灿秋 | PCR (polymerase chain reaction) plate cover |
| CN205741020U (en) * | 2016-05-10 | 2016-11-30 | 厦门致善生物科技股份有限公司 | A kind of hood-opening device being applicable to PCR multiple-connecting-pipe |
| CN205770866U (en) * | 2016-05-10 | 2016-12-07 | 厦门致善生物科技股份有限公司 | A kind of multi-joint lid of dry reagent and multi-joint sealing structure |
-
2017
- 2017-11-21 CN CN201711165299.6A patent/CN108085237A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140086881A1 (en) * | 2012-01-31 | 2014-03-27 | Kieu Hoang | Good healthy cells found in proteins, their applications, and process of making a medium to harvest the cells |
| CN203569075U (en) * | 2013-09-26 | 2014-04-30 | 钟灿秋 | PCR (polymerase chain reaction) plate cover |
| CN205741020U (en) * | 2016-05-10 | 2016-11-30 | 厦门致善生物科技股份有限公司 | A kind of hood-opening device being applicable to PCR multiple-connecting-pipe |
| CN205770866U (en) * | 2016-05-10 | 2016-12-07 | 厦门致善生物科技股份有限公司 | A kind of multi-joint lid of dry reagent and multi-joint sealing structure |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111974476A (en) * | 2020-09-11 | 2020-11-24 | 上海捷诺生物科技有限公司 | Freeze drying is with connecting test tube lid that stable leakproofness is good |
| WO2023072090A1 (en) * | 2021-10-25 | 2023-05-04 | 上海透景生命科技股份有限公司 | Polymerase chain reaction tube assembly |
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