CN108083896A - A kind of preparation method of bio-feritlizer - Google Patents
A kind of preparation method of bio-feritlizer Download PDFInfo
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- CN108083896A CN108083896A CN201711315841.1A CN201711315841A CN108083896A CN 108083896 A CN108083896 A CN 108083896A CN 201711315841 A CN201711315841 A CN 201711315841A CN 108083896 A CN108083896 A CN 108083896A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/90—Mixtures of one or more fertilisers with additives not having a specially fertilising activity for affecting the nitrification of ammonium compounds or urea in the soil
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/30—Layered or coated, e.g. dust-preventing coatings
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Abstract
The invention discloses a kind of preparation methods of bio-feritlizer, belong to fertilizer field.The present invention selects fresh cow dung, the raw material of powdered rice hulls, corn flour as fermenting organism fertilizer, it is environment friendly and pollution-free, by into material Vaccination Extraneous Source Microorganism microbial inoculum to improve fermentation efficiency, the phosphate-solubilizing bacteria of addition can dissolve the phosphate such as the tricalcium phosphate of indissoluble, ferric phosphate in soil and release water-soluble phosphorus, it is utilized for plant growth, potassium bacterium（Silicate bacteria）The minerals being made of silicate and aluminosilicate can be decomposed, the elements such as phosphorus, potassium, the silicon directly utilized for plant growth is released, increases soil fertility；Bio-feritlizer matrix and cupric pyrophosphate potassium are embedded in crosslinked sodium alginate, in this, as fertilizer kernel, water-retaining agent sodium alginate polyacrylic acid promotes the water retaining function of soil as fertiliser granulates coated fertilizer.The present invention solves the problem of current bio-fertilizer fertilizer efficiency is low, and water conservation and water holding function are poor, and slow release effect is bad.
Description
Technical field
The invention belongs to fertilizer fields, and in particular to a kind of preparation method of bio-feritlizer.
Background technology
Bio-feritlizer refers to microorganisms with specific functions and mainly with plant and animal residues（Such as feces of livestock and poultry, agricultural crop straw）It is next
The source and one kind being combined through harmless treatment, decomposed organic materials has both microbial manure and the fertilizer of organic fertilizer effect
Material.For a long time largely using chemical fertilizer and pesticide, the gas permeability and sponginess for having resulted in soil substantially reduce in China rural area, soil
The problem of earth caking is serious, the soil environment of crop growth receives very big destruction.If continue long-time service chemical fertilizer, gesture
The sustainable development of agricultural must be seriously affected.Therefore, farm manure, biological organic fertilizer are vigorously promoted the use in agricultural production,
Less with or without chemical fertilizer, it will the strong sustainable development for promoting agricultural.Not only containing a large amount of members necessary to plant in organic fertilizer
Element, trace element, also containing abundant organic nutrient, organic fertilizer is most comprehensive fertilizer.It is organic after organic fertilizer is manured into soil
Mass-energy effectively improves soil physical chemistry situation and biological nature, vegetable soil, enhances the fertilizer supply and preservation ability and buffer capacity of soil
Power is that good soil conditions are created in the growth of crop.After organic fertilizer decomposition, provide energy for soil microbial activity and support
Material promotes microbial activities, accelerates organic matter decomposition, the active material of generation etc. that can promote the growth of crop and improves agricultural product
Quality.Biological organic fertilizer nutrient is complete, can improve soil, improves and uses soil hardening caused by chemical fertilizer.Improve soil
Earth physicochemical character, enhancing soil water-retaining, fertilizer conservation, the ability of fertilizer.Beneficial microbe in biological organic fertilizer enter after soil with
Soil Microorganism forms mutual symbiosis Relationship With Proliferation, inhibits harmful bacteria and grows and be converted into beneficial bacterium, interacts, phase
Mutually promote, play the synergistic effect of group, beneficial bacterium generates substantial amounts of metabolite during growth and breeding, promotes organic matter
Decomposition and inversion, directly or indirectly can provide a variety of nutrition and pungent for crop, promote and regulation and control plant growth.It improves
Soil porosity, penetrating exchangeability and plant survival rate increase beneficial bacterium and edaphon and population.To people, animal, environment peace
Entirely, it is nontoxic, it is a kind of environment-friendly type fertilizer.Current biological organic fertilizer is included with feces of livestock and poultry etc. for primary raw material；With crops
Stalk etc. is primary raw material, although the biological organic fertilizer that these raw materials are prepared has certain effect, but as organic fertilizer feces of livestock and poultry
Easily decomposed when being fermented to be not thorough, slow release effect is bad, and contains the causes such as substantial amounts of lignin and cellulose in stalk
Close property ingredient, by microorganism spontaneous fermentation, since the type and quantity of contained indigenous microorganism are limited, microorganism is difficult to quickly divide
Solution utilizes, and bio-feritlizer water retaining function is again poor at present, and the fertilizer efficiency for resulting in biological organic fertilizer is low so as to cause crops
Low output.Therefore producing a kind of good bio-feritlizer of fertility high effect has the very big market demand.
The content of the invention
（6）The technical problems to be solved by the invention：Low, water conservation and water holding function for bio-fertilizer fertilizer efficiency currently on the market
The problem of difference, slow release effect is bad, provides a kind of preparation method of biological organic fertilizer.
In order to solve the above technical problems, the present invention is using technical solution as described below：
A kind of preparation method of bio-feritlizer, which is characterized in that the preparation method includes the following steps：
（1）Cow dung, powdered rice hulls, corn flour is taken to be uniformly mixed with water, obtains fermentation raw material, adds in plant ash carbon, sawdust and fermentation raw material
It is uniformly mixed, obtains mixture, mixture heap is entered in fermentation tank, add in one grade fermemtation composite bacteria agent and one grade fermemtation nutrition
Liquid obtains mixed fermentation material, will start to ferment after mixed fermentation material turning, every 2d turning and throwing onces, 45 ~ 55 DEG C of fermentations 14 ~
16d, cooling, obtains one grade fermemtation object；
（2）When temperature of having fermented for the first time is down to 35 ~ 40 DEG C, second order fermentation composite bacteria agent, second order fermentation nutrient solution are added in
Continue to ferment, every 1d turning and throwing onces, 40 ~ 45 DEG C of 7 ~ 9d of fermentation obtain second order fermentation object, take out second order fermentation object, dry, obtain biological
Fertilizer matrix；
（3）By dry bio-feritlizer matrix, cupric pyrophosphate potassium, sodium alginate is mixed with water, stirred evenly, and obtains bio-feritlizer base
Body mixed solution object, the mass fraction that bio-feritlizer matrix mixed solution object is added drop-wise to dropwise are 5% CaCl2In solution, into
Bio-feritlizer kernel and sodium alginate-- polyacrylic acid are placed in disk by row crosslinking, bio-feritlizer kernel after must embedding, and are turned
Water-retaining agent powder is uniformly wrapped in particle surface by dynamic disk, forms outer layer coating, the bio-feritlizer particle that coating is completed is 65
It is dry at ~ 70 DEG C, obtain bio-feritlizer.
The step（1）In fresh cow dung, powdered rice hulls, the mass ratio of corn flour and water be 5:1:2:8, fermentation raw material, plant ash
Carbon, the mass ratio of sawdust are 4:1:1, the mass ratio of mixture, one grade fermemtation composite bacteria agent and zymotic fluid is 3:2:5.
The step（1）Middle one grade fermemtation is with the preparation method of composite bacteria agent：By aspergillus oryzae in mass ratio 1:9 are inoculated into
PDA culture medium, 23 ~ 25 DEG C are cultivated 5 ~ 7 days, aspergillus oryzae culture are obtained, by pseudomonad in mass ratio 1:9 are inoculated into monad
30 ~ 35 DEG C of basal medium is cultivated 2 ~ 3 days, pseudomonad culture is obtained, by Escherichia coli in mass ratio 1:9 are inoculated into TSB trainings
It supports in base and cultivates 2 ~ 3 days for 30 ~ 35 DEG C, obtain culture of Escherichia coli, it is 0.9% physiology that three kinds of cultures are used to mass fraction respectively
Brine is diluted to 10-5, aspergillus oryzae, pseudomonad, Escherichia coli seed liquor is made, by aspergillus oryzae seed liquor, pseudomonad seed
Liquid, Escherichia coli seed liquor, wheat bran in mass ratio 2:2:1:3 are added to mixed fermentation in strain fermentation nutrient solution, 40 ~ 45 DEG C,
Ferment 24 ~ 72h, and fermentate is freeze-dried, obtains level-one composite bacteria agent.
The step（1）The formula of middle fermented nutritive liquid is according to the mass fraction, to take 10 ~ 15 parts of wheat bran, peptone 10 ~ 15
Part, 16 ~ 20 parts of millet powder, Na2CO31.2 ~ 2 parts, MgSO4·7H20.1 ~ 0.2 part of O, FeSO4·7H20.01 ~ 0.03 part of O,
2 ~ 6 parts of NaCl, 1000 parts of water, stir evenly, obtain strain fermentation nutrient solution.
The step（2）Middle second order fermentation is with the preparation method of composite bacteria agent：By azotobacter chroococcum in mass ratio 1:9 connect
To YEPD culture mediums, 28 ~ 30 DEG C are cultivated 2 ~ 3 days kind, azotobacter chroococcum culture are obtained, by bacillus megaterium in mass ratio 1:9
It is inoculated into 30 ~ 35 DEG C of LB basal mediums to cultivate 2 ~ 3 days, obtains bacillus megaterium culture, bacillusmusilaginosiengineering is pressed into matter
Measure ratio 1:9, which are inoculated into TSB culture mediums 30 ~ 35 DEG C, cultivates 2 ~ 3 days, bacillusmusilaginosiengineering culture is obtained, by three kinds of cultures
It is respectively 0.9% normal saline dilution to 10 with mass fraction-5, it is made, azotobacter chroococcum, bacillus megaterium, gel-shaped gemma
Bacillus seed liquor presses azotobacter chroococcum seed liquor, bacillus megaterium seed liquor, bacillusmusilaginosiengineering seed liquor, wheat bran
Mass ratio 1:1:1:2 are added to mixed fermentation in strain fermentation nutrient solution, and 40 ~ 45 DEG C, ferment 24 ~ 72h, fermentate is freezed dry
It is dry, obtain two level composite bacteria agent.
The step（2）The formula of middle second order fermentation nutrient solution is：According to the mass fraction, 12 ~ 15 parts of corn flour, cassava are taken
20 ~ 24 parts of powder, 8 ~ 10 parts of beef extract, 13 ~ 17 parts of glucose, Na2CO31.2 ~ 2 parts, MgSO4·7H20.2 ~ 0.5 part of O,
FeSO4·7H20.02 ~ 0.03 part of O, 3 ~ 5 parts of NaCl, 1000 parts of water, stir evenly, obtain second order fermentation nutrient solution.
The step（2）Middle one grade fermemtation object is with the mass ratio of composite bacteria agent and second order fermentation nutrient solution with second order fermentation
3:2:5。
The step（3）Middle cupric pyrophosphate process for synthesis of potassium is：Prepare the copper-bath and 12% that mass fraction is 15%
Jiao Pity acid potassium solutions, are stirred continuously, copper-bath are added drop-wise to potassium pyrophosphate solution dropwise under 23 ~ 25 DEG C of constant temperatures
In, until Cu2+With P2O7 4-Molar ratio reach 1:1, ageing for 24 hours, with distilled water will be aged sediment wash, dry to get.
The step（3）The synthesis of middle sodium alginate-- polyacrylic acid water-retaining agent：By sodium alginate in mass ratio 3:8 is molten
In distilled water, sodium alginate soln is obtained, is placed in the there-necked flask equipped with blender and gas-guide tube, by neutralizer acrylic acid, is triggered
Agent ammonium persulfate and crosslinking agent glutaraldehyde in mass ratio 3:2:5 mixing, obtain mixed solution, mixed solution are added dropwise to alginic acid
It is lasting to stir in sodium solution, it is passed through nitrogen 15 minutes, reactor is placed in 2 ~ 3h of reaction, reaction in 70 ~ 75 DEG C of water-bath terminates
Afterwards, it is dry, crush, cross 100 mesh sieves, collect sieving particle to get.
The step（3）The mass ratio of middle matrix, cupric pyrophosphate potassium, sodium alginate and water is 5:1:2:9, in bio-feritlizer
Core and sodium alginate-- polyacrylic acid in mass ratio 5:3.
Compared with other methods, advantageous effects are the present invention：
（7）The present invention selects fresh cow dung, the raw material of powdered rice hulls, corn flour as fermenting organism fertilizer, and environmental protection is pollution-free, fermentation
Go out to have both microbial manure and the fertilizer of organic fertilizer effect, improve it is a large amount of using chemical fertilizer and pesticide cause soil gas permeability and
The problem of sponginess substantially reduces, and soil block is serious；
（8）The present invention is by the way that into material, Vaccination Extraneous Source Microorganism microbial inoculum is to improve fermentation efficiency, and by secondary fermentation, addition is solid
Nitrogen bacterium, nitrogen source that can be in stable soil, the phosphate-solubilizing bacteria of addition can dissolve the tricalcium phosphate of indissoluble, ferric phosphate etc. in soil
Phosphate and release water-soluble phosphorus, for plant growth utilize, potassium bacterium（Silicate bacteria）It can decompose by silicate and aluminium
The minerals of silicate composition, release the elements such as phosphorus, potassium, the silicon directly utilized for plant growth, can effective organic fertilizer
Improve the physicochemical property of soil, increase soil fertility；
（9）Bio-feritlizer matrix and cupric pyrophosphate potassium are embedded in crosslinked sodium alginate by the present invention, in this, as in fertilizer
Core, water-retaining agent sodium alginate-- polyacrylic acid are prepared for a kind of slow with water retaining function as fertiliser granulates coated fertilizer
Bio-feritlizer is released, there is extraordinary slow-release function, promotes the water retaining function of soil.
Specific embodiment
Raw material:Using fresh cow dung, powdered rice hulls, corn flour as fermentation raw material, fresh cow dung, powdered rice hulls, corn flour and water are pressed
Mass ratio 5:1:2:8 are uniformly mixed.
Fermentation strain：Aspergillus oryzae, pseudomonad, Escherichia coli, azotobacter chroococcum, bacillus megaterium, gel-shaped bud
Spore bacillus.
The formula of one grade fermemtation nutrient solution：According to the mass fraction, 10 ~ 15 parts of wheat bran, 10 ~ 15 parts of peptone, millet powder are taken
16 ~ 20 parts, Na2CO31.2 ~ 2 parts, MgSO4·7H20.1 ~ 0.2 part of O, FeSO4·7H20.01 ~ 0.03 part of O, NaCl 2 ~ 6
Part, 1000 parts of water, stir evenly.
The preparation of one grade fermemtation composite bacteria agent：By aspergillus oryzae in mass ratio 1:9 are inoculated into PDA culture medium, 23 ~ 25 DEG C
Culture 5 ~ 7 days, obtains aspergillus oryzae culture, by pseudomonad vacation in mass ratio 1:9 are inoculated into 30 ~ 35 DEG C of monad basal medium
Culture 2 ~ 3 days, obtains pseudomonad culture, by Escherichia coli in mass ratio 1:9 are inoculated into 30 ~ 35 DEG C of cultures 2 in TSB culture mediums
~ 3 days, culture of Escherichia coli is obtained, three kinds of cultures are used to mass fraction respectively for 0.9% normal saline dilution to 10-5, it is made
Aspergillus oryzae, pseudomonad, Escherichia coli seed liquor, by aspergillus oryzae seed liquor, pseudomonad seed liquor, Escherichia coli seed liquor,
Wheat bran in mass ratio 2:2:1:3 are added to mixed fermentation in strain fermentation nutrient solution, and 40 ~ 45 DEG C, ferment 24 ~ 72h, by fermentate
Freeze-drying, obtains level-one composite bacteria agent.
The preparation of second order fermentation composite bacteria agent：By azotobacter chroococcum in mass ratio 1:9 are inoculated into YEPD culture mediums, 28 ~
30 DEG C are cultivated 2 ~ 3 days, azotobacter chroococcum culture are obtained, by bacillus megaterium in mass ratio 1:9 are inoculated into LB basal mediums
30 ~ 35 DEG C are cultivated 2 ~ 3 days, bacillus megaterium culture are obtained, by bacillusmusilaginosiengineering in mass ratio 1:9 are inoculated into TSB trainings
It supports in base and cultivates 2 ~ 3 days for 30 ~ 35 DEG C, obtain bacillusmusilaginosiengineering culture, be with mass fraction respectively by three kinds of cultures
0.9% normal saline dilution is to 10-5, it is made, azotobacter chroococcum, bacillus megaterium, bacillusmusilaginosiengineering seed liquor, by circle
Brown nitrogen-fixing bacteria seed liquor, bacillus megaterium seed liquor, bacillusmusilaginosiengineering seed liquor, wheat bran in mass ratio 1:1:1:2 add
Enter the mixed fermentation into strain fermentation nutrient solution, 40 ~ 45 DEG C, ferment 24 ~ 72h, and fermentate is freeze-dried, obtains two level compound bacteria
Agent.
Second order fermentation nutrient solution：According to the mass fraction, 12 ~ 15 parts of corn flour, 20 ~ 24 parts of tapioca starch, beef extract 8 ~ 10 are taken
Part, 13 ~ 17 parts of glucose, Na2CO31.2 ~ 2 parts, MgSO4·7H20.2 ~ 0.5 part of O, FeSO4·7H20.02 ~ 0.03 part of O,
3 ~ 5 parts of NaCl, 1000 parts of water, stir evenly.
Cupric pyrophosphate process for synthesis of potassium：The copper-bath and 12% Jiao Pity acid potassium solutions that mass fraction is 15% are prepared,
It is stirred continuously, copper-bath is added drop-wise to dropwise in potassium pyrophosphate solution under 23 ~ 25 DEG C of constant temperatures, until Cu2+With
P2O7 4-Molar ratio reach 1:1, ageing for 24 hours, with distilled water will be aged sediment wash, dry to get.
The synthesis of sodium alginate-- polyacrylic acid water-retaining agent：By sodium alginate in mass ratio 3:8 are dissolved in distilled water, obtain extra large
Solution of sodium alginate is placed in the there-necked flask equipped with blender and gas-guide tube, by neutralizer acrylic acid, initiator ammonium persulfate and friendship
Join agent glutaraldehyde in mass ratio 3:2:5 mixing, obtain mixed solution, mixed solution are added dropwise in sodium alginate soln, continue
Stirring, is passed through nitrogen 15 minutes, and reactor is placed in 2 ~ 3h of reaction in 70 ~ 75 DEG C of water-bath, after reaction, dry, crushing,
Cross 100 mesh sieves, collect sieving particle to get.
A kind of preparation method of bio-feritlizer of the present invention, includes the following steps：
1. take cow dung, powdered rice hulls, corn flour and water in mass ratio 5:1:2:8 be uniformly mixed, obtain fermentation raw material, by fermentation raw material,
Plant ash carbon, sawdust press in mass ratio 4:1:1 is uniformly mixed, and obtains mixture, mixture heap is entered in fermentation tank, adds in one grade fermemtation
With composite bacteria agent and zymotic fluid, the mass ratio of mixture, one grade fermemtation composite bacteria agent and zymotic fluid is 3:2:5, material turning
After start to ferment, every 2d turning and throwing onces, 45 ~ 55 DEG C of 14 ~ 16d of fermentation after cooling, obtain one grade fermemtation object.
2. after first time, which ferments, completes cooling, during 35 ~ 40 DEG C of temperature, second order fermentation composite bacteria agent is added in after supervention
Ferment, the mass ratio of one grade fermemtation object, second order fermentation composite bacteria agent and second order fermentation nutrient solution is 3:2:5, every 1d turning and throwing onces,
40 ~ 45 DEG C of 7 ~ 9d of fermentation, obtain second order fermentation object, take out second order fermentation object, dry, obtain bio-feritlizer matrix.
3. by dry bio-feritlizer matrix, cupric pyrophosphate potassium, sodium alginate and water in mass ratio 5:1:2:9 mixing, are stirred
It mixes uniformly, obtains bio-feritlizer matrix mixed solution, the mass fraction that bio-feritlizer matrix mixed solution is added drop-wise to dropwise is 5%
CaCl2It in solution, is crosslinked, forms the particle that grain size is about 4mm, bio-feritlizer kernel after must embedding, by bio-feritlizer
Kernel and sodium alginate-- polyacrylic acid in mass ratio 5:3 ratio is placed in disk, rotating circular disc, and water-retaining agent powder is equal
It is even to be wrapped in particle surface, outer layer coating is formed, the bio-feritlizer particle that coating is completed is dry at 65 ~ 70 DEG C, obtains bio-fertilizer
Material.
Example 1
Raw material:Using fresh cow dung, powdered rice hulls, corn flour as fermentation raw material, fresh cow dung, powdered rice hulls, corn flour and water are pressed into quality
Than 5:1:2:8 are uniformly mixed.
Fermentation strain：Aspergillus oryzae, pseudomonad, Escherichia coli, azotobacter chroococcum, bacillus megaterium, gel-shaped bud
Spore bacillus.
The formula of one grade fermemtation nutrient solution：It counts in parts by weight, takes 10 parts of wheat bran, 10 parts of peptone, 16 parts of millet powder,
Na2CO31.2 parts, MgSO4·7H20.1 part of O, FeSO4·7H20.01 part of O, 2 parts of NaCl, 1000 parts of water, stir evenly.
The preparation of one grade fermemtation composite bacteria agent：By aspergillus oryzae in mass ratio 1:9 are inoculated into PDA culture medium, 23 DEG C of cultures
5 days, aspergillus oryzae culture is obtained, by pseudomonad vacation in mass ratio 1:9, which are inoculated into 30 DEG C of monad basal medium, cultivates 2 days,
Pseudomonad culture is obtained, by Escherichia coli in mass ratio 1:9, which are inoculated into TSB culture mediums 30 DEG C, cultivates 2 days, obtains Escherichia coli
Three kinds of cultures are used mass fraction as 0.9% normal saline dilution to 10 by culture respectively-5, be made aspergillus oryzae, pseudomonad,
Escherichia coli seed liquor, by aspergillus oryzae seed liquor, pseudomonad seed liquor, Escherichia coli seed liquor, wheat bran in mass ratio 2:2:
1:3 are added to mixed fermentation in strain fermentation nutrient solution, and 40 DEG C, fermentation for 24 hours, fermentate is freeze-dried, obtains level-one compound bacteria
Agent.
The preparation of second order fermentation composite bacteria agent：By azotobacter chroococcum in mass ratio 1:9 are inoculated into YEPD culture mediums, and 28
DEG C culture 2 days, azotobacter chroococcum culture is obtained, by bacillus megaterium in mass ratio 1:9 are inoculated into 30 DEG C of LB basal mediums
Culture 2 days, obtains bacillus megaterium culture, by bacillusmusilaginosiengineering in mass ratio 1:9 are inoculated into TSB culture mediums 30 DEG C
Culture 2 days, obtain bacillusmusilaginosiengineering culture, by three kinds of cultures respectively with mass fraction for 0.9% normal saline dilution extremely
10-5, it is made, azotobacter chroococcum, bacillus megaterium, bacillusmusilaginosiengineering seed liquor, by azotobacter chroococcum seed liquor, huge
Bacillus seed liquor, bacillusmusilaginosiengineering seed liquor, wheat bran in mass ratio 1:1:1:2 are added in strain fermentation nutrient solution
Mixed fermentation, 40 DEG C, fermentation for 24 hours, fermentate is freeze-dried, obtains two level composite bacteria agent.
Second order fermentation nutrient solution：According to the mass fraction, 12 parts of corn flour, 20 parts of tapioca starch, 8 parts of beef extract, glucose are taken
13 parts, Na2CO31.2 parts, MgSO4·7H20.2 part of O, FeSO4·7H20.02 part of O, 3 parts of NaCl, 1000 parts of water, stirring are equal
It is even.
Cupric pyrophosphate process for synthesis of potassium：The copper-bath and 12% Jiao Pity acid potassium solutions that mass fraction is 15% are prepared,
It is stirred continuously, copper-bath is added drop-wise to dropwise in potassium pyrophosphate solution under 23 DEG C of constant temperatures, until Cu2+With P2O7 4-'s
Molar ratio reaches 1:1, ageing for 24 hours, with distilled water will be aged sediment wash, dry to get.
The synthesis of sodium alginate-- polyacrylic acid water-retaining agent：By sodium alginate in mass ratio 3:8 are dissolved in distilled water, obtain extra large
Solution of sodium alginate is placed in the there-necked flask equipped with blender and gas-guide tube, by neutralizer acrylic acid, initiator ammonium persulfate and friendship
Join agent glutaraldehyde in mass ratio 3:2:5 mixing, obtain mixed solution, mixed solution are added dropwise in sodium alginate soln, continue
Stirring, is passed through nitrogen 15 minutes, reactor is placed in 70 DEG C of water-bath and reacts 2h, and after reaction, dry, crushing crosses 100
Mesh sieve, collect sieving particle to get.
A kind of preparation method of bio-feritlizer of the present invention, includes the following steps：
4. take cow dung, powdered rice hulls, corn flour and water in mass ratio 5:1:2:8 be uniformly mixed, obtain fermentation raw material, by fermentation raw material,
Plant ash carbon, sawdust press in mass ratio 4:1:1 is uniformly mixed, and obtains mixture, mixture heap is entered in fermentation tank, adds in one grade fermemtation
With composite bacteria agent and zymotic fluid, the mass ratio of mixture, one grade fermemtation composite bacteria agent and zymotic fluid is 3:2:5, material turning
After start to ferment, every 2d turning and throwing onces, 45 DEG C of fermentation 14d after cooling, obtain one grade fermemtation object.
5. after first time, which ferments, completes cooling, during 35 DEG C of temperature, add in second order fermentation composite bacteria agent and continue to ferment, one
The mass ratio of grade fermentate, second order fermentation composite bacteria agent and second order fermentation nutrient solution is 3:2:5, every 1d turning and throwing onces, 40 DEG C
Ferment 7d, obtains second order fermentation object, takes out second order fermentation object, dry, obtains bio-feritlizer matrix.
6. by dry bio-feritlizer matrix, cupric pyrophosphate potassium, sodium alginate and water in mass ratio 5:1:2:9 mixing, are stirred
It mixes uniformly, obtains bio-feritlizer matrix mixed solution, the mass fraction that bio-feritlizer matrix mixed solution is added drop-wise to dropwise is 5%
CaCl2It in solution, is crosslinked, forms the particle that grain size is about 4mm, bio-feritlizer kernel after must embedding, by bio-feritlizer
Kernel and sodium alginate-- polyacrylic acid in mass ratio 5:3 ratio is placed in disk, rotating circular disc, and water-retaining agent powder is equal
It is even to be wrapped in particle surface, outer layer coating is formed, the bio-feritlizer particle that coating is completed is dry at 65 DEG C, obtains bio-feritlizer.
Example 2
Raw material:Using fresh cow dung, powdered rice hulls, corn flour as fermentation raw material, fresh cow dung, powdered rice hulls, corn flour and water are pressed into quality
Than 5:1:2:8 are uniformly mixed.
Fermentation strain：Aspergillus oryzae, pseudomonad, Escherichia coli, azotobacter chroococcum, bacillus megaterium, gel-shaped bud
Spore bacillus.
The formula of one grade fermemtation nutrient solution：According to the mass fraction, 15 parts of wheat bran is taken, 15 parts of peptone, 20 parts of millet powder,
Na2CO32 parts, MgSO4·7H20.2 part of O, FeSO4·7H20.03 part of O, 6 parts of NaCl, 1000 parts of water, stir evenly.
The preparation of one grade fermemtation composite bacteria agent：By aspergillus oryzae in mass ratio 1:9 are inoculated into PDA culture medium, 25 DEG C of cultures
7 days, aspergillus oryzae culture is obtained, by pseudomonad vacation in mass ratio 1:9, which are inoculated into 35 DEG C of monad basal medium, cultivates 3 days,
Pseudomonad culture is obtained, by Escherichia coli in mass ratio 1:9, which are inoculated into TSB culture mediums 35 DEG C, cultivates 3 days, obtains Escherichia coli
Three kinds of cultures are used mass fraction as 0.9% normal saline dilution to 10 by culture respectively-5, be made aspergillus oryzae, pseudomonad,
Escherichia coli seed liquor, by aspergillus oryzae seed liquor, pseudomonad seed liquor, Escherichia coli seed liquor, wheat bran in mass ratio 2:2:
1:3 are added to mixed fermentation in strain fermentation nutrient solution, and 45 DEG C, ferment 72h, and fermentate is freeze-dried, obtains level-one compound bacteria
Agent.
The preparation of second order fermentation composite bacteria agent：By azotobacter chroococcum in mass ratio 1:9 are inoculated into YEPD culture mediums, and 30
DEG C culture 3 days, azotobacter chroococcum culture is obtained, by bacillus megaterium in mass ratio 1:9 are inoculated into 35 DEG C of LB basal mediums
Culture 3 days, obtains bacillus megaterium culture, by bacillusmusilaginosiengineering in mass ratio 1:9 are inoculated into TSB culture mediums 35 DEG C
Culture 3 days, obtain bacillusmusilaginosiengineering culture, by three kinds of cultures respectively with mass fraction for 0.9% normal saline dilution extremely
10-5, it is made, azotobacter chroococcum, bacillus megaterium, bacillusmusilaginosiengineering seed liquor, by azotobacter chroococcum seed liquor, huge
Bacillus seed liquor, bacillusmusilaginosiengineering seed liquor, wheat bran in mass ratio 1:1:1:2 are added in strain fermentation nutrient solution
Mixed fermentation, 45 DEG C, ferment 72h, and fermentate is freeze-dried, obtains two level composite bacteria agent.
Second order fermentation nutrient solution：According to the mass fraction, 15 parts of corn flour, 24 parts of tapioca starch, 10 parts of beef extract, glucose are taken
17 parts, Na2CO32 parts, MgSO4·7H20.5 part of O, FeSO4·7H20.03 part of O, 5 parts of NaCl, 1000 parts of water, stirring are equal
It is even.
Cupric pyrophosphate process for synthesis of potassium：The copper-bath and 12% Jiao Pity acid potassium solutions that mass fraction is 15% are prepared,
It is stirred continuously, copper-bath is added drop-wise to dropwise in potassium pyrophosphate solution under 25 DEG C of constant temperatures, until Cu2+With P2O7 4-'s
Molar ratio reaches 1:1, ageing for 24 hours, with distilled water will be aged sediment wash, dry to get.
The synthesis of sodium alginate-- polyacrylic acid water-retaining agent：By sodium alginate in mass ratio 3:8 are dissolved in distilled water, obtain extra large
Solution of sodium alginate is placed in the there-necked flask equipped with blender and gas-guide tube, by neutralizer acrylic acid, initiator ammonium persulfate and friendship
Join agent glutaraldehyde in mass ratio 3:2:5 mixing, obtain mixed solution, mixed solution are added dropwise in sodium alginate soln, continue
Stirring, is passed through nitrogen 15 minutes, reactor is placed in 75 DEG C of water-bath and reacts 3h, and after reaction, dry, crushing crosses 100
Mesh sieve, collect sieving particle to get.
A kind of preparation method of bio-feritlizer of the present invention, includes the following steps：
7. take cow dung, powdered rice hulls, corn flour and water in mass ratio 5:1:2:8 be uniformly mixed, obtain fermentation raw material, by fermentation raw material,
Plant ash carbon, sawdust press in mass ratio 4:1:1 is uniformly mixed, and obtains mixture, mixture heap is entered in fermentation tank, adds in one grade fermemtation
With composite bacteria agent and zymotic fluid, the mass ratio of mixture, one grade fermemtation composite bacteria agent and zymotic fluid is 3:2:5, material turning
After start to ferment, every 2d turning and throwing onces, 55 DEG C of fermentation 16d after cooling, obtain one grade fermemtation object.
8. after first time, which ferments, completes cooling, during 40 DEG C of temperature, add in second order fermentation composite bacteria agent and continue to ferment, one
The mass ratio of grade fermentate, second order fermentation composite bacteria agent and second order fermentation nutrient solution is 3:2:5, every 1d turning and throwing onces, 45 DEG C
Ferment 9d, obtains second order fermentation object, takes out second order fermentation object, dry, obtains bio-feritlizer matrix.
9. by dry bio-feritlizer matrix, cupric pyrophosphate potassium, sodium alginate and water in mass ratio 5:1:2:9 mixing, are stirred
It mixes uniformly, obtains bio-feritlizer matrix mixed solution, the mass fraction that bio-feritlizer matrix mixed solution is added drop-wise to dropwise is 5%
CaCl2It in solution, is crosslinked, forms the particle that grain size is about 4mm, bio-feritlizer kernel after must embedding, by bio-feritlizer
Kernel and sodium alginate-- polyacrylic acid in mass ratio 5:3 ratio is placed in disk, rotating circular disc, and water-retaining agent powder is equal
It is even to be wrapped in particle surface, outer layer coating is formed, the bio-feritlizer particle that coating is completed is dry at 70 DEG C, obtains bio-feritlizer.
Example 3
Raw material:Using fresh cow dung, powdered rice hulls, corn flour as fermentation raw material, fresh cow dung, powdered rice hulls, corn flour and water are pressed into quality
Than 5:1:2:8 are uniformly mixed.
Fermentation strain：Aspergillus oryzae, pseudomonad, Escherichia coli, azotobacter chroococcum, bacillus megaterium, gel-shaped bud
Spore bacillus.
The formula of one grade fermemtation nutrient solution：According to the mass fraction, 12.5 parts of wheat bran, 12.5 parts of peptone, millet powder 18 are taken
Part, Na2CO31.6 parts, MgSO4·7H20.15 part of O, FeSO4·7H20.02 part of O, 4 parts of NaCl, 1000 parts of water, stirring are equal
It is even.
The preparation of one grade fermemtation composite bacteria agent：By aspergillus oryzae in mass ratio 1:9 are inoculated into PDA culture medium, 24 DEG C of cultures
6 days, aspergillus oryzae culture is obtained, by pseudomonad vacation in mass ratio 1:9 are inoculated into the 33 DEG C of cultures 2.5 of monad basal medium
My god, pseudomonad culture is obtained, by Escherichia coli in mass ratio 1:9, which are inoculated into TSB culture mediums 33 DEG C, cultivates 2.5 days, obtains greatly
Three kinds of cultures are used mass fraction as 0.9% normal saline dilution to 10 by enterobacteria culture respectively-5, aspergillus oryzae, vacation is made
Monad, Escherichia coli seed liquor, by aspergillus oryzae seed liquor, pseudomonad seed liquor, Escherichia coli seed liquor, wheat bran is by quality
Than 2:2:1:3 are added to mixed fermentation in strain fermentation nutrient solution, and 43 DEG C, ferment 48h, and fermentate is freeze-dried, obtains level-one
Composite bacteria agent.
The preparation of second order fermentation composite bacteria agent：By azotobacter chroococcum in mass ratio 1:9 are inoculated into YEPD culture mediums, and 29
DEG C culture 2.5 days, azotobacter chroococcum culture is obtained, by bacillus megaterium in mass ratio 1:9 are inoculated into LB basal mediums 33
DEG C culture 2.5 days, bacillus megaterium culture is obtained, by bacillusmusilaginosiengineering in mass ratio 1:9 are inoculated into TSB culture mediums
33 DEG C are cultivated 2.5 days, obtain bacillusmusilaginosiengineering culture, and it is 0.9% physiological saline that three kinds of cultures are used to mass fraction respectively
It is diluted to 10-5, it is made, azotobacter chroococcum, bacillus megaterium, bacillusmusilaginosiengineering seed liquor, by azotobacter chroococcum seed
Liquid, bacillus megaterium seed liquor, bacillusmusilaginosiengineering seed liquor, wheat bran in mass ratio 1:1:1:2 are added to strain fermentation
Mixed fermentation in nutrient solution, 43 DEG C, ferment 48h, and fermentate is freeze-dried, obtains two level composite bacteria agent.
Second order fermentation nutrient solution：According to the mass fraction, 13.5 parts of corn flour, 22 parts of tapioca starch, 9 parts of beef extract, grape are taken
15 parts of sugar, Na2CO31.6 parts, MgSO4·7H20.35 part of O, FeSO4·7H20.02 part of O, 4 parts of NaCl, 1000 parts of water, are stirred
It mixes uniformly.
Cupric pyrophosphate process for synthesis of potassium：The copper-bath and 12% Jiao Pity acid potassium solutions that mass fraction is 15% are prepared,
It is stirred continuously, copper-bath is added drop-wise to dropwise in potassium pyrophosphate solution under 24 DEG C of constant temperatures, until Cu2+With P2O7 4-'s
Molar ratio reaches 1:1, ageing for 24 hours, with distilled water will be aged sediment wash, dry to get.
The synthesis of sodium alginate-- polyacrylic acid water-retaining agent：By sodium alginate in mass ratio 3:8 are dissolved in distilled water, obtain extra large
Solution of sodium alginate is placed in the there-necked flask equipped with blender and gas-guide tube, by neutralizer acrylic acid, initiator ammonium persulfate and friendship
Join agent glutaraldehyde in mass ratio 3:2:5 mixing, obtain mixed solution, mixed solution are added dropwise in sodium alginate soln, continue
Stirring, is passed through nitrogen 15 minutes, reactor is placed in 73 DEG C of water-bath and reacts 2.5h, after reaction, dry, crushing, mistake
100 mesh sieves, collect sieving particle to get.
A kind of preparation method of bio-feritlizer of the present invention, includes the following steps：
10. take cow dung, powdered rice hulls, corn flour and water in mass ratio 5:1:2:8 be uniformly mixed, obtain fermentation raw material, by fermentation raw material,
Plant ash carbon, sawdust press in mass ratio 4:1:1 is uniformly mixed, and obtains mixture, mixture heap is entered in fermentation tank, adds in one grade fermemtation
With composite bacteria agent and zymotic fluid, the mass ratio of mixture, one grade fermemtation composite bacteria agent and zymotic fluid is 3:2:5, material turning
After start to ferment, every 2d turning and throwing onces, 50 DEG C of fermentation 15d after cooling, obtain one grade fermemtation object.
11. after first time, which ferments, completes cooling, during 37 DEG C of temperature, add in second order fermentation composite bacteria agent and continue to ferment,
The mass ratio of one grade fermemtation object, second order fermentation composite bacteria agent and second order fermentation nutrient solution is 3:2:5, every 1d turning and throwing onces, 43
DEG C fermentation 8d, obtains second order fermentation object, takes out second order fermentation object, dry, obtains bio-feritlizer matrix.
12. by dry bio-feritlizer matrix, cupric pyrophosphate potassium, sodium alginate and water in mass ratio 5:1:2:9 mixing, are stirred
It mixes uniformly, obtains bio-feritlizer matrix mixed solution, the mass fraction that bio-feritlizer matrix mixed solution is added drop-wise to dropwise is 5%
CaCl2It in solution, is crosslinked, forms the particle that grain size is about 4mm, bio-feritlizer kernel after must embedding, by bio-feritlizer
Kernel and sodium alginate-- polyacrylic acid in mass ratio 5:3 ratio is placed in disk, rotating circular disc, and water-retaining agent powder is equal
It is even to be wrapped in particle surface, outer layer coating is formed, the bio-feritlizer particle that coating is completed is dry at 67 DEG C, obtains bio-feritlizer.
13. reference examples：The biological organic fertilizer of company of Jinan City production.
14. the biological organic fertilizer of biological organic fertilizer obtained by examples detailed above and reference examples is detected, detection reference
NY884-2012.Yield test is carried out in certain experimental plot, experimental field soil property is sandy loam, and middle fertility, preceding crop is fresh kidney beans.
Test variety：Slow cabbage heart.Respectively base manure, top dressing are done using the biological organic fertilizer of the present invention and the biological organic fertilizer of reference examples.It presses
According to normal implantation methods, when harvest, tests its yield, and test result is shown in Table 1.
Table 1：
| Test event | Example 1 | Example 2 | Example 3 | Reference examples |
| Living bacteria count（CFU）Hundred million/gram | 1.6 | 1.5 | 1.8 | 0.3~0.7 |
| Organic matter % | 52 | 55 | 56 | 41~48 |
| Moisture % | 27 | 26 | 27 | 21 |
| Total nutrient % | 9.1 | 8.8 | 9.6 | 4~4.3 |
| Yield kg | 32.1 | 33.6 | 34.8 | 23.1~25.4 |
Summary, the fertility of bio-feritlizer of the invention is high, and the yield of crops can be more improved after use, is worthy to be popularized.
Claims (10)
1. a kind of preparation method of bio-feritlizer, which is characterized in that the preparation method includes the following steps：
（1）Cow dung, powdered rice hulls, corn flour is taken to be uniformly mixed with water, obtains fermentation raw material, adds in plant ash carbon, sawdust and fermentation raw material
It is uniformly mixed, obtains mixture, mixture heap is entered in fermentation tank, add in one grade fermemtation composite bacteria agent and one grade fermemtation nutrition
Liquid obtains mixed fermentation material, will start to ferment after mixed fermentation material turning, every 2d turning and throwing onces, 45 ~ 55 DEG C of fermentations 14 ~
16d, cooling, obtains one grade fermemtation object；
（2）When temperature of having fermented for the first time is down to 35 ~ 40 DEG C, adds in second order fermentation and close Jun Ji ﹑ second order fermentation nutrient solutions with Fu
Continue to ferment, every 1d turning and throwing onces, 40 ~ 45 DEG C of 7 ~ 9d of fermentation obtain second order fermentation object, take out second order fermentation object, dry, obtain biological
Fertilizer matrix；
（3）By dry bio-feritlizer matrix, cupric pyrophosphate potassium, sodium alginate is mixed with water, stirred evenly, and obtains bio-feritlizer base
Body mixed solution, the mass fraction that bio-feritlizer matrix mixed solution is added drop-wise to dropwise are 5% CaCl2In solution, handed over
Bio-feritlizer kernel and sodium alginate-- polyacrylic acid are placed in disk by connection, bio-feritlizer kernel after must embedding, and rotate circle
Water-retaining agent powder is uniformly wrapped in particle surface by disk, forms outer layer coating, the bio-feritlizer particle that coating is completed is 65 ~ 70
It is dry at DEG C, obtain bio-feritlizer.
2. the preparation method of bio-feritlizer according to claim 1, which is characterized in that the step（1）In fresh cow dung, rice
The mass ratio of shell powder, corn flour and water is 5:1:2:8, fermentation raw material, plant ash carbon, the mass ratio of sawdust are 4:1:1, mixture,
The mass ratio of one grade fermemtation composite bacteria agent and zymotic fluid is 3:2:5.
3. the preparation method of bio-feritlizer according to claim 1, which is characterized in that the step（1）Middle one grade fermemtation
It is with the preparation method of composite bacteria agent：By aspergillus oryzae in mass ratio 1:9 are inoculated into PDA culture medium, and 23 ~ 25 DEG C are cultivated 5 ~ 7 days,
Aspergillus oryzae culture is obtained, by pseudomonad in mass ratio 1:9, which are inoculated into 30 ~ 35 DEG C of monad basal medium, cultivates 2 ~ 3 days, obtains
Pseudomonad culture, by Escherichia coli in mass ratio 1:9, which are inoculated into TSB culture mediums 30 ~ 35 DEG C, cultivates 2 ~ 3 days, obtains large intestine
Three kinds of cultures are used mass fraction as 0.9% normal saline dilution to 10 by alphacterium culture respectively-5, aspergillus oryzae, false list is made
Born of the same parents bacterium, Escherichia coli seed liquor, by aspergillus oryzae seed liquor, pseudomonad seed liquor, Escherichia coli seed liquor, wheat bran is in mass ratio
2:2:1:3 are added to mixed fermentation in strain fermentation nutrient solution, and 40 ~ 45 DEG C, ferment 24 ~ 72h, and fermentate is freeze-dried, is obtained
Level-one composite bacteria agent.
4. the preparation method of bio-feritlizer according to claim 1, which is characterized in that the step（1）In it is Nutrious fermented
The formula of liquid is according to the mass fraction, to take 10 ~ 15 parts of wheat bran, 10 ~ 15 parts of peptone, 16 ~ 20 parts of millet powder, Na2CO3 1.2~2
Part, MgSO4·7H20.1 ~ 0.2 part of O, FeSO4·7H20.01 ~ 0.03 part of O, 2 ~ 6 parts of NaCl, 1000 parts of water, stir evenly,
Obtain strain fermentation nutrient solution.
5. the preparation method of bio-feritlizer according to claim 1, which is characterized in that the step（2）Middle second order fermentation
It is with the preparation method of composite bacteria agent：By azotobacter chroococcum in mass ratio 1:9 are inoculated into YEPD culture mediums, 28 ~ 30 DEG C of cultures 2 ~ 3
My god, azotobacter chroococcum culture is obtained, by bacillus megaterium in mass ratio 1:9 are inoculated into 30 ~ 35 DEG C of cultures 2 of LB basal mediums
~ 3 days, bacillus megaterium culture is obtained, by bacillusmusilaginosiengineering in mass ratio 1:9 are inoculated into TSB culture mediums 30 ~ 35 DEG C
Culture 2 ~ 3 days, obtains bacillusmusilaginosiengineering culture, and it is 0.9% normal saline dilution that three kinds of cultures are used to mass fraction respectively
To 10-5, it is made, azotobacter chroococcum, bacillus megaterium, bacillusmusilaginosiengineering seed liquor, by azotobacter chroococcum seed liquor, huge
Bacterium anthracoides seed liquor, bacillusmusilaginosiengineering seed liquor, wheat bran in mass ratio 1:1:1:2 are added to strain fermentation nutrient solution
Middle mixed fermentation, 40 ~ 45 DEG C, ferment 24 ~ 72h, and fermentate is freeze-dried, obtains two level composite bacteria agent.
6. the preparation method of bio-feritlizer according to claim 1, which is characterized in that the step（2）In
The formula of second order fermentation nutrient solution is：According to the mass fraction, 12 ~ 15 parts of corn flour, 20 ~ 24 parts of tapioca starch, beef extract 8 are taken
~ 10 parts, 13 ~ 17 parts of glucose, Na2CO31.2 ~ 2 parts, MgSO4·7H20.2 ~ 0.5 part of O, FeSO4·7H2O 0.02~0.03
Part, 3 ~ 5 parts of NaCl, 1000 parts of water, stir evenly, obtain second order fermentation nutrient solution.
7. the preparation method of bio-feritlizer according to claim 1, which is characterized in that the step（2）Middle one grade fermemtation
The mass ratio of object and second order fermentation composite bacteria agent and second order fermentation nutrient solution is 3:2:5.
8. the preparation method of bio-feritlizer according to claim 1, which is characterized in that the step（3）Middle cupric pyrophosphate
Process for synthesis of potassium is：The copper-bath and 12% Jiao Pity acid potassium solutions that mass fraction is 15% are prepared, is stirred continuously, 23 ~ 25
Copper-bath is added drop-wise to dropwise in potassium pyrophosphate solution under DEG C constant temperature, until Cu2+With P2O7 4-Molar ratio reach
1:1, ageing for 24 hours, with distilled water will be aged sediment wash, dry to get.
9. the preparation method of bio-feritlizer according to claim 1, which is characterized in that the step（3）Middle sodium alginate-
The synthesis of-polyacrylic acid water-retaining agent：By sodium alginate in mass ratio 3:8 are dissolved in distilled water, obtain sodium alginate soln, are placed in dress
In the there-necked flask for having blender and gas-guide tube, neutralizer acrylic acid, initiator ammonium persulfate and crosslinking agent glutaraldehyde are pressed into quality
Than 3:2:5 mixing, obtain mixed solution, mixed solution are added dropwise in sodium alginate soln, lasting to stir, and are passed through nitrogen 15 and divide
Reactor is placed in 2 ~ 3h of reaction in 70 ~ 75 DEG C of water-bath by clock, and after reaction, 100 mesh sieves are crossed in dry, crushing, are collected
Sieve particle to get.
10. the preparation method of bio-feritlizer according to claim 1, which is characterized in that the step（3）Middle matrix, it is burnt
The mass ratio of cupric phosphate potassium, sodium alginate and water is 5:1:2:9, bio-feritlizer kernel presses matter with sodium alginate-- polyacrylic acid
Measure ratio 5:3.
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