CN108069570B - Method for removing carbon, nitrogen and phosphorus in aquaculture wastewater - Google Patents

Method for removing carbon, nitrogen and phosphorus in aquaculture wastewater Download PDF

Info

Publication number
CN108069570B
CN108069570B CN201711435650.9A CN201711435650A CN108069570B CN 108069570 B CN108069570 B CN 108069570B CN 201711435650 A CN201711435650 A CN 201711435650A CN 108069570 B CN108069570 B CN 108069570B
Authority
CN
China
Prior art keywords
culture
reaction unit
wastewater
nitrogen
phosphorus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201711435650.9A
Other languages
Chinese (zh)
Other versions
CN108069570A (en
Inventor
钟玉鸣
刘晖
王琴
童英林
柳建良
李焕垲
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guangdong Fengnonghui Technology Development Co.,Ltd.
Original Assignee
Zhongkai University of Agriculture and Engineering
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zhongkai University of Agriculture and Engineering filed Critical Zhongkai University of Agriculture and Engineering
Priority to CN201711435650.9A priority Critical patent/CN108069570B/en
Publication of CN108069570A publication Critical patent/CN108069570A/en
Application granted granted Critical
Publication of CN108069570B publication Critical patent/CN108069570B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/105—Phosphorus compounds
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/10—Inorganic compounds
    • C02F2101/16—Nitrogen compounds, e.g. ammonia
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/30—Organic compounds
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
    • C02F2203/006—Apparatus and plants for the biological treatment of water, waste water or sewage details of construction, e.g. specially adapted seals, modules, connections
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/08—Chemical Oxygen Demand [COD]; Biological Oxygen Demand [BOD]
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/10—Solids, e.g. total solids [TS], total suspended solids [TSS] or volatile solids [VS]
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/16—Total nitrogen (tkN-N)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Botany (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Hydrology & Water Resources (AREA)
  • Mycology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Cell Biology (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a method for removing carbon, nitrogen and phosphorus in aquaculture wastewater, which comprises a central reaction unit, wherein the accompanying flora is added by introducing the aquaculture wastewater; the peripheral reaction unit is used for adding chlorella by introducing culture wastewater, and wastewater flowing out of the central reaction unit is introduced into the peripheral reaction unit; the method for separating and culturing the associated flora comprises the following steps: taking aquaculture wastewater, removing suspended matters and large plankton, performing suction filtration to obtain concentrated solution, filtering, collecting precipitate, transferring to an M0 liquid culture medium for enrichment culture, centrifuging the obtained primary culture, inoculating the obtained precipitate into an M0 solid culture medium for purification and separation, then placing the precipitate into an M0 liquid culture medium for culture, centrifuging after the culture is finished, adding the obtained precipitate into an M1 liquid culture medium for amplification culture, and centrifuging after the culture is finished, wherein the obtained precipitate is an associated flora. The invention takes the treatment of the culture wastewater and the function of strengthening the growth of microalgae into consideration, not only finishes the removal of nitrogen and phosphorus elements in the culture wastewater, but also realizes the functions of recovering microalgae resources and the like.

Description

Method for removing carbon, nitrogen and phosphorus in aquaculture wastewater
Technical Field
The invention belongs to the field of environment-friendly devices, and particularly relates to a method for removing carbon, nitrogen and phosphorus in aquaculture wastewater.
Background
At present, the water environment pollution problem in China is increasingly serious, and nitrogen, phosphorus, organic matters and novel toxic pollutants are in endless. The requirement of the country on total nitrogen emission control of coastal land level and above cities in 'ten water entries' is met, so that the requirement on water treatment is improved by a higher pattern. The livestock breeding wastewater has the characteristics of high COD, strong biodegradability and high nitrogen and phosphorus content. Because nitrogen and phosphorus pollution of the breeding wastewater is serious, a large amount of breeding wastewater is discharged into a local water system to cause serious influence, about 1 hundred million tons of poultry excrement are generated in China every year, more than 500 million tons of livestock breeding wastewater are discharged in Guangzhou city every day, and the ecological balance of the water body is seriously damaged.
Generally, the removal of nitrogen by microorganisms is an economical means, especially in the fields of aquaculture and waterfowl farming. The denitrification process comprises an ammonia oxidation process, a nitrification process, a denitrification process and an anaerobic ammonia oxidation continuous process, and how to stably and organically combine several microorganisms is also a difficulty in removing nitrogen.
Disclosure of Invention
The invention aims to provide a method for removing carbon, nitrogen and phosphorus in aquaculture wastewater according to the defects in the prior art.
In order to achieve the purpose, the invention provides a method for removing carbon, nitrogen and phosphorus in aquaculture wastewater, which comprises the following steps:
a central reaction unit with the volume of 100-200L is used for pretreatment of the aquaculture wastewater by introducing the aquaculture wastewater and adding associated flora;
the peripheral reaction unit is 800-1000L in volume and is used for further removing water pollutants;
the central reaction unit is nested in the peripheral reaction units and is provided with a first water inlet and a first water outlet; the peripheral reaction unit is provided with a second water inlet and a second water outlet, the first water outlet is connected with the second water inlet through a pipeline, and a sedimentation device and a valve are arranged on the pipeline;
the volume of the precipitation device is about 5L, and the precipitation device is used for precipitating the aquaculture wastewater before introducing the aquaculture wastewater into the peripheral reaction unit;
the main function of the sedimentation device is to control the suspended matters of the effluent and provide treated influent water for the peripheral reaction device. The sedimentation tank volume, the typical residence time, is 2-4 hours. After precipitation, the feed water ss entering the peripheral reaction unit is not higher than 200 mg/L.
The circulating water pump has the functions that: and according to the retention time of the peripheral reaction unit, periodically extracting the inlet water from the supernatant of the sedimentation tank to enter the peripheral reaction unit.
The method for separating and culturing the associated flora comprises the following steps:
taking aquaculture wastewater, removing suspended matters and large plankton, performing suction filtration to obtain concentrated solution, filtering, collecting precipitate, transferring to an M0 liquid culture medium for enrichment culture, centrifuging the obtained primary culture, inoculating the obtained precipitate into an M0 solid culture medium for purification and separation, then placing the precipitate into an M0 liquid culture medium for culture, centrifuging after the culture is finished, adding the obtained precipitate into an M1 liquid culture medium for amplification culture, and centrifuging after the culture is finished, wherein the obtained precipitate is an associated flora;
wherein each liter of the M0 liquid culture medium comprises the following components: NaNO3 1500mg，K2HPO4 0.04mg，MgSO4·7H2O 75mg，CaCl2·2H2O36 mg, citric acid 6mg, EDTA 1mg, Na2CO3 20mg；
The M0 solid culture medium comprises the following components per liter: NaNO3 1500mg，K2HPO4 0.04mg，MgSO47H2O 75mg，CaCl2 2H2O36 mg, citric acid 6mg, EDTA 1mg, Na2CO320mg, 2 wt% of agar powder;
the M1 liquid culture medium comprises the following components per liter: CH (CH)3COONa 3.68g，Na2HPO4 28.73mg，NH4Cl 57.27mg，MgSO4 131.82mg，K2SO4 26.74mg，CaCl2·2H2O17.2 mg, pH 7.0.
As a preferable scheme of the method for removing carbon, nitrogen and phosphorus from the aquaculture wastewater, in the central reaction unit, the volume ratio of the aquaculture wastewater to the associated flora is 100: 1.
As a preferable scheme of the method for removing carbon, nitrogen and phosphorus in the aquaculture wastewater, the enrichment culture is carried out for 2-3 days under the conditions of illumination all day and 25 ℃, and the shaking is carried out for 2-3 times every day.
As a preferable scheme of the method for removing carbon, nitrogen and phosphorus in the aquaculture wastewater, the enlarged culture is carried out for 3-4 days under the conditions of non-illumination and 25 ℃.
The operation thought of the method for removing carbon, nitrogen and phosphorus in the aquaculture wastewater is that the central reaction unit and the peripheral reaction units respectively and independently operate, and after the central reaction unit and the peripheral reaction units reach a certain standard (when microalgae are cultured to a certain scale), the two reaction units are communicated with the precipitation device to intermittently operate, and the specific operation method comprises the following steps:
introducing the culture wastewater and associated flora into a central reaction unit through a first water inlet, starting independent circulating operation, firstly setting hydraulic retention time to be 4-7 days, setting the hydraulic retention time to be 2-3 days when COD removal rate reaches more than 50% and total nitrogen and total phosphorus removal rates respectively reach more than 20%, and completely starting the central reaction unit when the COD removal rate reaches more than 50% and the total nitrogen and total phosphorus removal rates respectively reach more than 50%;
introducing culture wastewater into the peripheral reaction units, adding chlorella, placing and culturing for 4-6 days, completely starting the peripheral reaction units when the removal rate of COD is more than 50% and the removal rates of total nitrogen and total phosphorus are respectively more than 30%, and starting the two reaction units and the precipitation device to be communicated and intermittently operated;
the aquaculture wastewater treated by the central reaction unit flows into the precipitation device through the first water outlet, then the water in the central reaction unit stops entering the precipitation device, the aquaculture wastewater entering the precipitation device is precipitated for 2-4 h (the aquaculture wastewater cannot enter the peripheral reaction unit before the precipitation is finished), then the aquaculture wastewater is pumped into the peripheral reaction unit through the circulating water pump for treatment, and the treated water is discharged through the second water outlet; when the water in the precipitation device is completely discharged into the peripheral reaction units, the water in the central reaction unit enters the precipitation device again for precipitation, and the operation is repeated.
Furthermore, in the peripheral reaction unit, the volume ratio of the culture wastewater to the chlorella is 500-1000: 1.
Compared with the prior art, the invention has the following beneficial effects:
the method provided by the invention has the functions of treating the culture wastewater and strengthening the growth of microalgae by using associated flora, can remove nitrogen and phosphorus elements in the culture wastewater, and can realize functions of microalgae resource recovery and the like.
Drawings
FIG. 1 is a schematic cross-sectional view of the apparatus for removing carbon, nitrogen and phosphorus from aquaculture wastewater according to the present invention.
Fig. 2 is a schematic top view of the apparatus shown in fig. 1.
FIG. 3 is a graph showing the reaction efficiency when the associated microorganisms and microalgae act together, compared with that when they act alone.
Figure 4 is a 7 day removal rate result for the inventive device.
Description of reference numerals:
a 10-center reaction unit; 12-a first water inlet; 14-a first water outlet; 20-a precipitation device; 30-a circulating water pump; 40-a peripheral reaction unit; 42-a second water inlet; 44-second water outlet.
Detailed Description
In order to make the objects, technical solutions and advantageous technical effects of the present invention clearer, the present invention is further described in detail with reference to the following embodiments. It should be understood that the embodiments described in this specification are only for the purpose of illustrating the invention and are not to be construed as limiting the invention, and the parameters, proportions and the like of the embodiments may be suitably selected without materially affecting the results.
Example 1
The method for removing carbon, nitrogen and phosphorus in the aquaculture wastewater (figures 1 and 2) comprises the following steps:
the central reaction unit 10 is used for pretreating the aquaculture wastewater by introducing the aquaculture wastewater and adding the associated flora;
the peripheral reaction unit 40 is used for introducing the wastewater flowing out of the central reaction unit 10 into the peripheral reaction unit 40 for further removing water pollutants;
the central reaction unit 10 is nested in the peripheral reaction unit 40, and the central reaction unit 10 is provided with a first water inlet 12 and a first water outlet 14; the peripheral reaction unit 40 is provided with a second water inlet 42 and a second water outlet 44, the first water outlet 12 is connected with the second water inlet 42 through a pipeline, and the pipeline is provided with a precipitation device 20 and a valve;
the precipitation device 20 performs precipitation before the culture wastewater is introduced into the peripheral reaction unit 40;
the method for separating and culturing the associated flora comprises the following steps:
taking aquaculture wastewater, removing suspended matters and large plankton, performing suction filtration to obtain concentrated solution, filtering, collecting precipitate, transferring to an M0 liquid culture medium for enrichment culture, centrifuging the obtained primary culture, inoculating the obtained precipitate into an M0 solid culture medium for purification and separation, then placing the precipitate into an M0 liquid culture medium for culture, centrifuging after the culture is finished, adding the obtained precipitate into an M1 liquid culture medium for amplification culture, and centrifuging after the culture is finished, wherein the obtained precipitate is an associated flora;
wherein, each liter of M0 liquid culture medium comprises the following components: NaNO3 1500mg，K2HPO4 0.04mg，MgSO4·7H2O 75mg，CaCl2·2H2O36 mg, citric acid 6mg, EDTA 1mg, Na2CO3 20mg；
The M0 solid culture medium comprises the following components per liter: NaNO3 1500mg，K2HPO4 0.04mg，MgSO4 7H2O 75mg，CaCl2 2H2O36 mg, citric acid 6mg, EDTA 1mg, Na2CO320mg, 2 wt% of agar powder;
the M1 liquid culture medium comprises the following components per liter: CH (CH)3COONa 3.68g，Na2HPO4 28.73mg，NH4Cl 57.27mg，MgSO4 131.82mg，K2SO4 26.74mg，CaCl2·2H2O17.2 mg, pH 7.0.
The volume of the central reaction unit 10 is about 100-200L, and the volume of the central reaction unit is introduced into the volume of the medium diluted by M1 to be 1: 1 (by volume ratio) of the culture wastewater to the volume of the wastewater 1: 100 adding the associated flora A, and starting to adopt the circulation operation without accessing the peripheral reaction unit 40. The flow rate of the central reaction unit 10 is set according to the hydraulic retention time control at 4-7 days. Measuring COD, total nitrogen and total phosphorus every week, when the removal rate of COD is more than 50%, respectively reaching more than 20%, shortening hydraulic retention time to 2-3 days, measuring COD, total nitrogen and total phosphorus every 2-3 days, when the removal rate of COD is more than 50%, respectively reaching more than 20%, changing into introducing raw water of aquaculture wastewater and continuously feeding water, setting hydraulic retention time to 2-3 days, measuring COD, total nitrogen and total phosphorus every 2-3 days, respectively reaching more than 50%, and starting the central reaction unit 10. The central reaction unit 10 has the functions of pretreatment of the aquaculture wastewater and reinforcement of the peripheral reaction units.
The volume of the peripheral reaction unit 40 is 800-;
the aquaculture wastewater treated by the central reaction unit 10 flows into the precipitation device 20(5L) through the first water outlet 12 to be precipitated for 2-4 h, then passes through the second water inlet 42 by the circulating water pump 30 and enters the peripheral reaction unit 40 to be treated, and the treated water is discharged through the second water outlet 44.
Example 2
This example employs a method of screening microalgae from wastewater.
Taking 15-20L of wastewater from a waterfowl culture wastewater factory, removing suspended matters by conventional isolation, aerating for 30min, and precipitating for 30 min; then, collecting the precipitate on a filter membrane through suction filtration or centrifugation; washing the precipitate with sterile water for 1-2 times, transferring to M0 liquid culture medium, performing enrichment culture under the conditions of illumination throughout the day and 25 deg.C for 2-3 days, and shaking for 2-3 times daily to obtain primary culture. Inoculating the primary culture in M0 liquid culture medium, culturing for 7 days under the conditions of illumination in the whole day, 25 ℃ and 150rpm, measuring the removal efficiency of nitrate, total nitrogen and total phosphorus every week, and replacing the liquid culture medium M0 after the removal efficiency of nitrate, total nitrogen and total phosphorus respectively reaches 50%, 40% and 40%, until the removal efficiency of nitrate, total nitrogen and total phosphorus respectively reaches 80%, 50% and 60%, and obtaining the screened chlorella culture.
Diluting the screened culture, finding chlorella cells with a low magnification magnifier, sucking out, centrifuging at 5000 rpm, collecting precipitate, inoculating the precipitate to M0 solid culture medium, streaking, purifying, and separating to obtain wastewater chlorella for 2-3 times. Putting the obtained microalgae (chlorella waste water) into 500ml of M0 liquid culture medium, performing illumination all day long, culturing for 3-5 days at 25 ℃, taking out 5ml of liquid, centrifuging at 5000 r/min, adding M1 liquid culture medium, culturing for 3 days again at 25 ℃ under non-illumination conditions, centrifuging at 5000 r/min, taking out the precipitate, replacing M1 liquid culture medium, repeating for 2 times, and finally centrifuging to obtain the precipitate as an associated flora, wherein the associated flora is used for adding into a central reaction unit.
Starting and operating the central reaction unit:
the central reaction unit has the functions of pretreating waterfowl wastewater and strengthening the peripheral reaction units. The pollutant concentration of the aquaculture wastewater is respectively as follows: 40-48mg/L of total nitrogen, 31-33mg/L of ammonia nitrogen, 36-40mg/L of total phosphorus, 800-900mg/L of COD, 6-7 of pH and less than 200mg/L of sewage solid suspended matters.
The volume of the central reaction unit is about 150-200L, and the volume of the central reaction unit is introduced into a reactor diluted by a culture medium M1 to be 1: 1 (by volume ratio) of the waterfowl wastewater to the volume of the wastewater 1: 100 adding the associated flora, starting to adopt internal circulation operation, and not accessing the peripheral reaction unit. The flow rate of the central reaction unit is controlled to be set at 4-7 days according to the hydraulic retention time. Measuring COD, total nitrogen and total phosphorus every week, when the removal rate of COD is more than 50%, respectively reaching more than 20%, shortening hydraulic retention time to 2 days, measuring COD, total nitrogen and total phosphorus every 2-3 days, when the removal rate of COD is more than 50%, respectively reaching more than 20%, changing into introducing raw water of aquaculture wastewater and continuously feeding water, setting hydraulic retention time to 2-3 days, measuring COD, total nitrogen and total phosphorus every 2-3 days, respectively reaching more than 50% when the removal rate of COD is more than 50%, respectively reaching more than 50%, and starting the reaction unit. The reactor suspension concentration (SS) should be maintained at 1000-2000 mg/L. After the effluent passes through the precipitation device, the effluent ss is controlled to be 80mg/L-100mg/L and is transferred into a peripheral reaction unit through a circulating water pump.
Starting and operating the peripheral reaction unit:
the volume of the peripheral reaction unit is 800L, the effluent from the precipitation device is firstly received during operation, the full volume is about more than 95%, the water inlet is stopped after the full volume is reached, and the chlorella is added into the peripheral reaction unit (the inoculation volume ratio is that the wastewater: the chlorella seeds are 500: 1-1000: 1). After 1-2 weeks of pre-culture, ss is maintained at 1200-2000 mg/L, COD, total nitrogen and total phosphorus are measured every week, when the removal rate of COD is up to more than 50%, the removal rates of total nitrogen and total phosphorus are respectively up to more than 30%, water is continuously fed from the sedimentation tank instead, the retention time during operation is set to 7 days, then COD, total nitrogen and total phosphorus are measured every week, when the removal rate of COD is up to more than 50%, the removal rates of total nitrogen and total phosphorus are respectively up to more than 30%, the starting of the peripheral reaction unit is completed, water is continuously fed from the central reaction unit, and the retention time is set to 4-6 days.
The peripheral reaction unit may harvest chlorella whenever ss exceeds 2000 mg/L. A certain amount of microalgae associated bacteria is mixed in the wastewater, and the microalgae associated bacteria and the chlorella are used together for removing water pollutants to form a synergistic effect.
The experiment shows that: when in use, a control group of waste water chlorella and associated flora is arranged at the same time, and the degradation efficiency of the waste water chlorella promoted by the associated flora is reflected (figure 3). It can be seen that the removal efficiency of the combination of the associated flora and the chlorella as the wastewater is the highest, wherein the removal efficiency of the total nitrogen and the ammonia nitrogen is improved by 3-5% compared with the removal efficiency of the chlorella without the associated flora, and the removal efficiency of the COD is improved by more than 20%. The chlorella as the waste water can obtain better culture waste water removing capacity under the coordination of the associated flora.
FIG. 4 shows that the removal efficiency of the actual process integrated unit is somewhat improved over that of the simple mix, and that the proportioning operation also maintains a high removal efficiency, especially after a certain period of operation, while the simple proportioning has a somewhat reduced rate. (simple mixing means that the associated flora is added in proportion during the operation of the reactor, but not through the proportioning operation of the reactor). The treatment efficiency of the integrated device culture and the simple culture is greatly different. When the CODcr concentration of inlet water is 800mg/L, ammonia nitrogen is 31mg/L, total nitrogen is 46mg/L, and total phosphorus is 36mg/L, the CODcr concentration of outlet water is 64mg/L, ammonia nitrogen is 3.1mg/L, total nitrogen is 5.1mg/L, and total phosphorus is 3.6mg/L after 7 days of the integrated device, showing that the integrated device has higher culture removal efficiency.
TABLE 1 reaction apparatus removal efficiency
Figure GDA0002723976160000081
Example 3
Chlorella pyrenoidosa was purchased and named Chlorella pyrenoidosa as Latin.
Taking 15-20L of wastewater from a waterfowl culture wastewater factory, removing suspended matters by conventional isolation, aerating for 30min, and precipitating for 30 min; adding purchased chlorella into the mixture according to the volume ratio of 1: 100. performing enrichment culture under the conditions of illumination all day and 25 ℃ for 2-3 days, and shaking for 2-3 times every day to obtain a primary culture. Inoculating the primary culture in M0 liquid culture medium, culturing for 7 days under the conditions of illumination at 25 ℃ and 150rpm throughout the day, measuring the removal efficiency of nitrate, total nitrogen and total phosphorus every week, and replacing the liquid culture medium M0 after the removal efficiency of nitrate, total nitrogen and total phosphorus respectively reaches 50%, 40% and 40%, until the removal efficiency of nitrate, total nitrogen and total phosphorus respectively reaches 80%, 50% and 60%, thereby obtaining the expanded culture.
The volume of the central reaction unit is about 200L, and the volume of the central reaction unit is added into a reactor diluted by a culture medium M1 to be 1: 1 (by volume ratio) of the waterfowl wastewater to the volume of the wastewater 1: 100 adding the associated flora, starting to adopt the circulating operation, and not accessing the peripheral reaction unit. The flow rate of the central reaction unit is controlled to be set at 7 days according to the hydraulic retention time. Measuring COD, total nitrogen and total phosphorus every week, when the removal rate of COD is more than 40%, the removal rate of total nitrogen and total phosphorus respectively reaches more than 20-25%, shortening hydraulic retention time to 3 days, measuring COD, total nitrogen and total phosphorus every 2 days, when the removal rate of COD reaches more than 50%, the removal rate of total nitrogen and total phosphorus respectively reaches more than 20%, changing to introducing waterfowl wastewater raw water, changing to continuous water feeding (from a water inlet to a water outlet 1), setting the hydraulic retention time to 2-3 days, measuring COD, total nitrogen and total phosphorus every 2-3 days, when the removal rate of COD reaches more than 50%, the removal rate of total nitrogen and total phosphorus respectively reaches more than 50%, and starting a central reaction unit. The central reaction unit is used for pretreatment of waterfowl wastewater and strengthening the function of the peripheral reaction unit. Before treatment, the pollutant concentrations of the aquaculture wastewater are respectively as follows: 40-48mg/L of total nitrogen, 31-33mg/L of ammonia nitrogen, 36-40mg/L of total phosphorus, 800-900mg/L of COD and 6-7 of pH value, and controlling the suspended solid in the effluent to be 200 mg/L.
Other reference example 1 was run.
The results of the runs are shown in table 2. When the CODcr concentration of inlet water is 800mg/L, ammonia nitrogen is 28mg/L, total nitrogen is 38mg/L and total phosphorus is 30mg/L, the CODcr concentration of outlet water is 64mg/L, ammonia nitrogen is 2.5mg/L, total nitrogen is 7.6mg/L and total phosphorus is 5.7mg/L after 7 days of the integrated device, and the purchased chlorella can also be suitable for the device.
Table 2 example 3 reaction apparatus removal efficiency
Figure GDA0002723976160000091
Appropriate changes and modifications to the embodiments described above will become apparent to those skilled in the art from the disclosure and teachings of the foregoing description. Therefore, the present invention is not limited to the specific embodiments disclosed and described above, and some modifications and variations of the present invention should fall within the scope of the claims of the present invention. Furthermore, although specific terms are employed herein, they are used in a generic and descriptive sense only and not for purposes of limitation.

Claims (6)

1. A method for removing carbon, nitrogen and phosphorus in aquaculture wastewater is characterized by comprising the following steps:
a central reaction unit with the volume of 100-200L is used for pretreatment of the aquaculture wastewater by introducing the aquaculture wastewater and adding associated flora;
the peripheral reaction unit is 800-1000L in volume and is used for further removing water pollutants;
the central reaction unit is nested in the peripheral reaction units and is provided with a first water inlet and a first water outlet; the peripheral reaction unit is provided with a second water inlet and a second water outlet, the first water outlet is connected with the second water inlet through a pipeline, and a sedimentation device and a valve are arranged on the pipeline;
the volume of the precipitation device is 5L, and the precipitation device is used for precipitating the culture wastewater before introducing the culture wastewater into the peripheral reaction unit, introducing the precipitated culture wastewater into the peripheral reaction unit, and adding chlorella into the peripheral reaction unit;
the method for separating and culturing the associated flora comprises the following steps:
taking aquaculture wastewater, removing suspended matters and large plankton, performing suction filtration to obtain concentrated solution, filtering, collecting precipitate, transferring to an M0 liquid culture medium for enrichment culture, centrifuging the obtained primary culture, inoculating the obtained precipitate into an M0 solid culture medium for purification and separation, then placing the precipitate into an M0 liquid culture medium for culture, centrifuging after the culture is finished, adding the obtained precipitate into an M1 liquid culture medium for amplification culture, and centrifuging after the culture is finished, wherein the obtained precipitate is an associated flora;
wherein each liter of the M0 liquid culture medium comprises the following components: NaNO3 1500 mg，K2HPO40.04mg，MgSO4·7H2O 75mg，CaCl2·2H2O36 mg, citric acid 6mg, EDTA 1mg, Na2CO3 20 mg；
The M0 solid culture medium comprises the following components per liter: NaNO3 1500 mg，K2HPO4 0.04mg，MgSO47H2O 75mg，CaCl2 2H2O36 mg, citric acid 6mg, EDTA 1mg, Na2CO320mg, 2 wt% of agar powder;
the M1 liquid culture medium comprises the following components per liter: CH (CH)3COONa 3.68g，Na2HPO4 28.73mg，NH4Cl 57.27mg，MgSO4 131.82 mg，K2SO4 26.74 mg，CaCl2·2H2O17.2 mg, pH 7.0.
2. The method for removing carbon, nitrogen and phosphorus in aquaculture wastewater according to claim 1, wherein the volume ratio of the aquaculture wastewater to the associated flora in the central reaction unit is 100: 1.
3. The method for removing carbon, nitrogen and phosphorus in aquaculture wastewater according to claim 1, wherein the enrichment culture is carried out under the conditions of illumination in the whole day and 25 ℃ for 2-3 days, and the shaking is carried out 2-3 times per day.
4. The method for removing carbon, nitrogen and phosphorus in aquaculture wastewater according to claim 1, wherein the enlarged culture is carried out for 3-4 days under the non-illumination and 25 ℃.
5. The method for removing carbon, nitrogen and phosphorus in aquaculture wastewater according to claim 1,
introducing the culture wastewater and associated flora into a central reaction unit through a first water inlet, starting independent circulating operation, firstly setting hydraulic retention time to be 4-7 days, setting the hydraulic retention time to be 2-3 days when COD removal rate reaches more than 50% and total nitrogen and total phosphorus removal rates respectively reach more than 20%, and completely starting the central reaction unit when the COD removal rate reaches more than 50% and the total nitrogen and total phosphorus removal rates respectively reach more than 50%;
introducing culture wastewater into the peripheral reaction unit, adding chlorella, placing and culturing for 4-6 days, and completely starting the peripheral reaction unit when the removal rate of COD is more than 50% and the removal rates of total nitrogen and total phosphorus are respectively more than 30%;
the aquaculture wastewater treated by the central reaction unit flows into the precipitation device through the first water outlet to precipitate for 2-4 hours, then passes through the second water inlet by the circulating water pump, enters the peripheral reaction unit to be treated, and the treated water is discharged through the second water outlet.
6. The method for removing carbon, nitrogen and phosphorus in aquaculture wastewater according to claim 5, wherein the volume ratio of the aquaculture wastewater to the chlorella in the peripheral reaction unit is 500-1000: 1.
CN201711435650.9A 2017-12-26 2017-12-26 Method for removing carbon, nitrogen and phosphorus in aquaculture wastewater Active CN108069570B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201711435650.9A CN108069570B (en) 2017-12-26 2017-12-26 Method for removing carbon, nitrogen and phosphorus in aquaculture wastewater

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201711435650.9A CN108069570B (en) 2017-12-26 2017-12-26 Method for removing carbon, nitrogen and phosphorus in aquaculture wastewater

Publications (2)

Publication Number Publication Date
CN108069570A CN108069570A (en) 2018-05-25
CN108069570B true CN108069570B (en) 2020-12-08

Family

ID=62155800

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201711435650.9A Active CN108069570B (en) 2017-12-26 2017-12-26 Method for removing carbon, nitrogen and phosphorus in aquaculture wastewater

Country Status (1)

Country Link
CN (1) CN108069570B (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109081447B (en) * 2018-08-24 2021-06-22 仲恺农业工程学院 Method for removing nitrogen and phosphorus in culture wastewater by combining chlorella, acinetobacter and pseudomonas
CN109110927B (en) * 2018-08-24 2021-06-22 仲恺农业工程学院 Method for removing nitrogen and phosphorus in culture wastewater by combining chlorella and acinetobacter

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001170671A (en) * 1999-12-15 2001-06-26 Research Institute Of Innovative Technology For The Earth Biological treatment method and device for waste water
CN104004645A (en) * 2014-05-29 2014-08-27 江苏大学 Two-phase system and method for organic wastewater hydrolytic acidification coupled microalgae culture
CN105018336A (en) * 2015-06-30 2015-11-04 浙江清华长三角研究院 Apparatus for culturing microalgae by using waste water
CN105152466A (en) * 2015-08-07 2015-12-16 仲恺农业工程学院 Method for treating waste water of aquatic bird cultivation through utilizing microalgae
CN105621789A (en) * 2015-12-21 2016-06-01 浙江清华长三角研究院 Microalgal culture based biogas liquid treatment device and method

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001170671A (en) * 1999-12-15 2001-06-26 Research Institute Of Innovative Technology For The Earth Biological treatment method and device for waste water
CN104004645A (en) * 2014-05-29 2014-08-27 江苏大学 Two-phase system and method for organic wastewater hydrolytic acidification coupled microalgae culture
CN105018336A (en) * 2015-06-30 2015-11-04 浙江清华长三角研究院 Apparatus for culturing microalgae by using waste water
CN105152466A (en) * 2015-08-07 2015-12-16 仲恺农业工程学院 Method for treating waste water of aquatic bird cultivation through utilizing microalgae
CN105621789A (en) * 2015-12-21 2016-06-01 浙江清华长三角研究院 Microalgal culture based biogas liquid treatment device and method

Also Published As

Publication number Publication date
CN108069570A (en) 2018-05-25

Similar Documents

Publication Publication Date Title
CN102336498B (en) Nitrogen-phosphorus wastewater treating method by sequencing batch reactor coupled photobioreactor
CN102976566A (en) System and method for treating high-concentration landfill leachate in aerobic composting site
CN103626352A (en) Advanced treatment and recycling process and apparatus for sewage reaching standard
CN103787547A (en) Treatment method for aged landfill leachate
CN106430845A (en) Kitchen garbage wastewater treatment apparatus
CN103408201B (en) The treatment process of trade effluent during silicon wafer mortar reclaims
CN110902962A (en) Wastewater system for treating melamine adhesive film paper
WO2020015458A1 (en) Treatment method for waste water from livestock and poultry
CN112591951A (en) Catalytic electrolysis purification system and purification method for municipal sewage
CN101781056B (en) Treatment method of waste papermaking water
CN103058392A (en) Method and device removing nitrates from water body by single-stage autotrophic denitrification
CN108069570B (en) Method for removing carbon, nitrogen and phosphorus in aquaculture wastewater
CN209989143U (en) Denitrifying phosphorus removal coupling vibration oxygen deficiency MBR device
CN109081447B (en) Method for removing nitrogen and phosphorus in culture wastewater by combining chlorella, acinetobacter and pseudomonas
CN114291942A (en) Catalytic electrolysis municipal sewage purification system and purification method
CN106587349B (en) Treatment device for high-ammonia-nitrogen high-organic-matter corn deep processing wastewater
CN210711180U (en) Cephalosporin antibiotic drug production wastewater treatment system and biochemical treatment system
CN111995182A (en) Resourceful treatment system and method for using potato starch wastewater as carbon source for sewage plant supply
CN207792814U (en) A kind of denitrification dephosphorization system
CN111153551A (en) Municipal sewage treatment device and treatment process
CN216737990U (en) Deep treatment system capable of achieving III-class water standard of surface water
CN206985970U (en) It is a kind of to improve biochemical and deeply treating wastewater system using Powdered Activated Carbon
CN202529948U (en) Treatment and recycling system for coking phenol-cyanogen wastewater
CN105198153A (en) Method suitable for urban sewage treatment
CN109110927B (en) Method for removing nitrogen and phosphorus in culture wastewater by combining chlorella and acinetobacter

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant
TR01 Transfer of patent right
TR01 Transfer of patent right

Effective date of registration: 20221118

Address after: 511500 028, Zone B, College Student Innovation and Entrepreneurship Incubation Base, Qingyuan Campus, Guangdong Science and Trade Vocational College, No. 36, Zhongsu Road, Dongcheng Street, Qingyuan District, Guangdong Province

Patentee after: Guangdong Fengnonghui Technology Development Co.,Ltd.

Address before: No. 501, Zhongkai Road, Haizhu District, Guangzhou, Guangdong 510225

Patentee before: ZHONGKAI University OF AGRICULTURE AND ENGINEER