CN108065036A - Applications of the bacillus marinus N6-2 in pig feed additive is prepared - Google Patents
Applications of the bacillus marinus N6-2 in pig feed additive is prepared Download PDFInfo
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- CN108065036A CN108065036A CN201711339426.XA CN201711339426A CN108065036A CN 108065036 A CN108065036 A CN 108065036A CN 201711339426 A CN201711339426 A CN 201711339426A CN 108065036 A CN108065036 A CN 108065036A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
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Abstract
The present invention provides a kind of new applications of bacillus marinus N6 2.Application of the first public microorganism of the present invention as pig feed additive, applies it in pannage, can play the role of improving intramuscular fat content in pork, so as to obtain snowflake pork, gained pork is made to have more market value.
Description
Technical field
The present invention relates to field of feed additive technology, are being prepared more particularly, to a kind of bacillus marinus N6-2
Application in pig feed additive.
Background technology
Snowflake meat is a noun for embodying meat quality, because fat deposition is between meat fiber, is formed with fiber of red muscle
Apparent marbling, exactly likes flakes and gains the name, and the meat of the snowflake meat infertile oiliness also not bavin when edible, health is again
Deliciousness, therefore the welcome of consumers in general is obtained, it is considered as the treasure of meat.Many people, which both know about beef, " snowflakes beef ",
But few people know that pork also has " snowflake pork ".This is because under normal conditions only when intramuscular fat content in pork
During more than 8%, apparent snowflake is just formed, only when intramuscular fat content reaches more than 10%, just forms apparent vaporific snowflake,
And intramuscular fat content is between 2~6% in general pork, its marbling unobvious, also can not form apparent snowflake at this time
Shape so " snowflake pork " is more difficult to get, is also just rarely known by the people.Insider adds in additive to regulate and control usually in feed
The growth and development of pig, but do not have the feed addition that can improve meat quality and obtain snowflake pork in currently available technology also
Agent.
The content of the invention
It is an object of the invention to overcome the shortcomings of existing feed addictive, one kind of bacillus marinus N6-2 is provided
Bacillus marinus N6-2 is applied in pig feed additive, to improve meat quality, plays raising pork by new application
The effect of middle intramuscular fat content so as to obtain snowflake pork, makes gained pork have more market value.
It is a further object to provide a kind of pig feed additives for including the bacillus marinus N6-2.
It is also another object of the present invention to provide a kind of pannages for including the bacillus marinus N6-2.
It is also another object of the present invention to provide a kind of methods for improving meat quality.
To realize the purpose of the invention described above, the technical solution adopted by the present invention is：
The present inventor has found bacillus marinus N6-2 being applied in pig feed additive by Animal experiment, can
Improve meat quality, fat is promoted to be accumulated in muscle, play the role of improving intramuscular fat content in pork, obtain snowflake pig
Meat.
Therefore, applications of the bacillus marinus N6-2 in pig feed additive is prepared, should be in protection scope of the present invention
It is interior.
Wherein, the bacillus marinus N6-2, for existing known strain, Classification And Nomenclature is Bacillus sp.N6-
2, from China typical culture collection center, preserving number is CCTCC NO:M2014586, preservation address are Wuhan, China city
Wuchang Luo Jia Shan Wuhan University.
Present invention can apply to general pig kinds, are preferably but not limited to, and the species of the pig is Hainan pig.Preferably, institute
State the hind shank that pork is pig.
Preferably, the pig feed additive is the feed addictive that can improve meat quality.
Specifically, the improvement meat quality refers to intramuscular fat content >=8% for making pork.In addition, more specifically,
The meat quality that improves also refers to the protein content for improving pork.Preferably, the myoglobins in pork, myoglobins are improved
Concentration it is higher, pork is redder, and snowflake pork can more be highlighted by being commonly called as " Najran Red ".
In addition, a kind of pig feed additive and pannage for including the bacillus marinus N6-2, also all in the present invention
Protection domain in.
One boar food is prepared by the following raw material calculated by mass percentage：Bacillus marinus N6-2 0.5%
~ 10%, protein sources raw material 80%~90%, fat source raw material 5~16%, multi-vitamins 2~5%.
The preparation method of the pannage can refer to following steps：
S1. culture medium is prepared, culture is enlarged to bacillus marinus N6-2, then air-dries, crush through solid fermentation, obtain
Obtain bacillus marinus N6-2 microorganism finished products；
S2. by proportioning by bacillus marinus N6-2 microorganisms finished product and protein sources raw material, fat source raw material, multi-vitamins into
Row mixing, sieving is to get the pannage after air-drying.
Preferably, the pannage is prepared by the following raw material calculated by mass percentage：Bacillus marinus N6-
2 6%, protein sources raw material 82%, fat source raw material 10%, multi-vitamins 2%.
Preferably, the protein sources raw material is made of fish meal, black soldier flies larva powder, PURE WHEY.
Preferably, the fish meal：Black soldier flies larva powder：The mass ratio of PURE WHEY is 1：1~2：1~5.
It is highly preferred that the fish meal：Black soldier flies larva powder：The mass ratio of PURE WHEY is 1：2：4.
The multi-vitamins into be grouped by weight generally：Vitamin B10.025wt%, riboflavin 0.1wt%,
Vitamin B6Hydrochloride 0.075wt%, niacin 0.25wt%, inositol 39.9wt%, calcium pantothenate 0.5wt%, biotin 0.25wt%, leaf
Sour 0.025wt%, vitamin K30.5wt%, vitamin B12 0.25wt%, vitamine A acetate 0.25wt%, vitamin D3
0.625wt%, vitamin E acetate 7.25wt%, media fibers element 50wt%.
The fat source raw material is animal fat, is preferably lard such as butter, lard.
In addition, the present invention also provides a kind of methods for improving meat quality, pig is fed using pannage of the present invention.
Specifically, the method is：The pig at least 180 days of the week old of four week old ~ five is fed using the pannage.
Compared with prior art, the beneficial effects of the invention are as follows：
The present invention provides a kind of new applications of the bacillus marinus N6-2.The first public microorganism of the present invention
It as the application of pig feed additive, applies it in pannage, the work for improving intramuscular fat content in pork can be played
With, so as to obtain snowflake pork, make gained pork have more market value.
Specific embodiment
The present invention is further illustrated With reference to embodiment.
The present invention is further described by specific examples below, it should be understood that specific implementation described herein
Example is only used for explaining the present invention, is not intended to limit the present invention, and can carry out various change in the range of right of the present invention restriction
Become.Test method used in following embodiments is conventional method unless otherwise specified；Used material, reagent etc.,
Unless otherwise specified, the reagent and material commercially to obtain.
The bacillus marinus N6-2 used described in the present embodiment for existing known bacterium, derives from Chinese Typical Representative
Culture collection, preserving number are CCTCC NO:M2014586, preservation address are big for Wuhan, China city Wuchang Luo Jia Shan Wuhan
It learns.
Embodiment 1
One boar food is prepared by the following raw material calculated by mass percentage：Bacillus marinus N6-2 0.5%, egg
White source raw material 86%, fat source raw material 10%, multi-vitamins 3.5%.
Wherein, fish meal in protein sources raw material：Black soldier flies larva powder：The mass ratio of PURE WHEY is 1：2：4.Fat source is former
Expect for lard.
Multi-vitamins are by weight into being grouped into：Vitamin B10.025wt%, riboflavin 0.1wt%, vitamin B6
Hydrochloride 0.075wt%, niacin 0.25wt%, inositol 39.9wt%, calcium pantothenate 0.5wt%, biotin 0.25wt%, folic acid
0.025wt%, vitamin K30.5wt%, vitamin B12 0.25wt%, vitamine A acetate 0.25wt%, vitamin D3
0.625wt%, vitamin E acetate 7.25wt%, media fibers element 50wt%.
The pannage is prepared by following steps：
S1. culture medium is prepared, culture is enlarged to bacillus marinus N6-2, then air-dries, crush through solid fermentation, obtain
Obtain bacillus marinus N6-2 microorganism finished products；
S2. proportioning is pressed by bacillus marinus N6-2 microorganisms finished product and protein sources raw material, fat source raw material, multi-vitamins
Mixed, air-dry after sieving to get.
Embodiment 2
One boar food is prepared by the following raw material calculated by mass percentage：Bacillus marinus N6-2 6%, albumen
Source raw material 82%, fat source raw material 10%, multi-vitamins 2%.
Wherein, fish meal in protein sources raw material：Black soldier flies larva powder：The mass ratio of PURE WHEY is 1：2：4.Fat source is former
Expect for lard.Multi-vitamins it is same as Example 1 into being grouped into.
Preparation method is same as Example 1.
Embodiment 3
One boar food is prepared by the following raw material calculated by mass percentage：Bacillus marinus N6-2 10%, albumen
Source raw material 80%, fat source raw material 8%, multi-vitamins 2%.
Wherein, fish meal in protein sources raw material：Black soldier flies larva powder：The mass ratio of PURE WHEY is 1：2：4.Fat source is former
Expect for lard.Multi-vitamins it is same as Example 1 into being grouped into.
Preparation method is same as Example 1.
Embodiment 4
One boar food is prepared by the following raw material calculated by mass percentage：Bacillus marinus N6-2 6%, albumen
Source raw material 82%, fat source raw material 10%, multi-vitamins 2%.
Wherein, fish meal in protein sources raw material：Black soldier flies larva powder：The mass ratio of PURE WHEY is 1：1：1.Fat source is former
Expect for lard.Multi-vitamins it is same as Example 1 into being grouped into.
Preparation method is same as Example 1.
Embodiment 5
One boar food is prepared by the following raw material calculated by mass percentage：Bacillus marinus N6-2 6%, albumen
Source raw material 82%, fat source raw material 10%, multi-vitamins 2%.
Wherein, fish meal in protein sources raw material：Black soldier flies larva powder：The mass ratio of PURE WHEY is 12：5.Fat source is former
Expect for lard.Multi-vitamins it is same as Example 1 into being grouped into.
Preparation method is same as Example 1.
Embodiment 6
One boar food is prepared by the following raw material calculated by mass percentage：Bacillus marinus N6-2 6%, albumen
Source raw material 82%, fat source raw material 10%, multi-vitamins 2%.
Wherein, fish meal in protein sources raw material：Black soldier flies larva powder：The mass ratio of PURE WHEY is 1：2：1.Fat source is former
Expect for lard.Multi-vitamins it is same as Example 1 into being grouped into.
Preparation method is same as Example 1.
Comparative example 1
One boar food is prepared by the following raw material calculated by mass percentage：Bacillus marinus N6-2 6%, albumen
Source raw material 82%, fat source raw material 10%, multi-vitamins 2%.
Wherein, protein sources raw material only has fish meal and PURE WHEY, fish meal：The mass ratio of PURE WHEY is 1： 4.Fat
Fat source raw material is lard.Multi-vitamins it is same as Example 1 into being grouped into.
Preparation method is same as Example 1.
Comparative example 2
One boar food is prepared by the following raw material calculated by mass percentage：Bacillus marinus N6-2 6%, albumen
Source raw material 82%, fat source raw material 10%, multi-vitamins 2%.
Wherein, protein sources raw material be dregs of beans and PURE WHEY, dregs of beans：The mass ratio of PURE WHEY is 1： 4.Fat
Source raw material is lard.Multi-vitamins it is same as Example 1 into being grouped into.
Preparation method is same as Example 1.
Comparative example 3
One boar food is prepared by the following raw material calculated by mass percentage：Bacillus marinus N6-2 6%, albumen
Source raw material 65%, fat source raw material 27%, multi-vitamins 2%.
Wherein, fish meal in protein sources raw material：Black soldier flies larva powder：The mass ratio of PURE WHEY is 1：2：4.Fat source is former
Expect for lard.Multi-vitamins it is same as Example 1 into being grouped into.
Preparation method is same as Example 1.
Testing inspection
In order to study the effect of additive for microbe feedstuff of the present invention, selection pig kind is Hainan pig as subjects, test bar
Part is：The pig of 100 five week old is chosen, is randomly divided into 10 groups, every group is 10 pigs.Wherein 9 groups are respectively adopted above-described embodiment
With the pannage being prepared described in comparative example, remaining one group is blank control example（Using not adding additive for microbe feedstuff
Pannage）, it is normal to feed 180 days.
Afterwards, the hind shank of pig is taken（Same area）, observe its character field headquarters of going forward side by side and support composition detection, result such as table 1
Shown in table 2：
1 appearance character of table
2 testing result of table
Obviously, the above embodiment of the present invention is only intended to clearly illustrate example of the present invention, and is not to this hair
The restriction of bright embodiment.For those of ordinary skill in the art, can also do on the basis of the above description
Go out other various forms of variations or variation.There is no necessity and possibility to exhaust all the enbodiments.It is all in the present invention
Spirit and principle within all any modification, equivalent and improvement made etc., should be included in the guarantor of the claims in the present invention
Within the scope of shield.
Claims (9)
1. applications of the bacillus marinus N6-2 in pig feed additive is prepared.
2. application according to claim 1, which is characterized in that the pig feed additive is that can improve meat quality
Feed addictive.
3. application according to claim 2, which is characterized in that the improvement meat quality refers to the intramuscular fat for making pork
Fat content >=8%.
4. a kind of pig feed additive, which is characterized in that include the bacillus marinus N6-2.
A 5. boar food, which is characterized in that be prepared by the following raw material calculated by mass percentage：Ocean gemma bar
Bacterium N6-2 0.5% ~ 10%, protein sources raw material 80%~90%, fat source raw material 5~16%, multi-vitamins 2~5%.
6. pannage according to claim 5, which is characterized in that prepared by the following raw material calculated by mass percentage
It obtains：Bacillus marinus N6-2 6%, protein sources raw material 82%, fat source raw material 10%, multi-vitamins 2%.
7. pannage according to claim 5, which is characterized in that the protein sources raw material by fish meal, black soldier flies larva powder,
PURE WHEY forms.
8. pannage according to claim 7, which is characterized in that the fish meal：Black soldier flies larva powder：PURE WHEY
Mass ratio is 1：1~2：1~5.
A kind of 9. method for improving meat quality, which is characterized in that fed using claim 5 ~ 8 any one of them pannage
Pig.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711339426.XA CN108065036B (en) | 2017-12-14 | 2017-12-14 | Application of marine bacillus N6-2 in preparation of pig feed additive |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711339426.XA CN108065036B (en) | 2017-12-14 | 2017-12-14 | Application of marine bacillus N6-2 in preparation of pig feed additive |
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| CN108065036A true CN108065036A (en) | 2018-05-25 |
| CN108065036B CN108065036B (en) | 2021-08-31 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103734543A (en) * | 2014-01-15 | 2014-04-23 | 双胞胎（集团）股份有限公司 | Biological feed additive capable of improving meat quality and promoting growth for fattening pigs |
| CN106011113A (en) * | 2016-04-28 | 2016-10-12 | 中国水产科学研究院黄海水产研究所 | Beta-glucanase generated by Bacillus marinus and preparation method thereof |
| CN106615792A (en) * | 2016-12-14 | 2017-05-10 | 安佑生物科技集团股份有限公司 | High digestibility shelled black soldier fly insect powder-containing piglet care feed |
| CN106719422A (en) * | 2016-12-31 | 2017-05-31 | 枞阳县恒祥生态农业有限公司 | A kind of chicken house large area feeding chicken in largely scale method |
-
2017
- 2017-12-14 CN CN201711339426.XA patent/CN108065036B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103734543A (en) * | 2014-01-15 | 2014-04-23 | 双胞胎（集团）股份有限公司 | Biological feed additive capable of improving meat quality and promoting growth for fattening pigs |
| CN106011113A (en) * | 2016-04-28 | 2016-10-12 | 中国水产科学研究院黄海水产研究所 | Beta-glucanase generated by Bacillus marinus and preparation method thereof |
| CN106615792A (en) * | 2016-12-14 | 2017-05-10 | 安佑生物科技集团股份有限公司 | High digestibility shelled black soldier fly insect powder-containing piglet care feed |
| CN106719422A (en) * | 2016-12-31 | 2017-05-31 | 枞阳县恒祥生态农业有限公司 | A kind of chicken house large area feeding chicken in largely scale method |
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| CN108065036B (en) | 2021-08-31 |
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| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Ma Xingshou Inventor after: Guo Shengcheng Inventor before: Ma Xingshou Inventor before: Guo Shengcheng Inventor before: Chen Zepeng |
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Effective date of registration: 20210415 Address after: 570000 room 505, Building 29, General Hospital of state farms, 48 baishuitang Road, Longhua District, Haikou City, Hainan Province Applicant after: Guo Shengcheng Address before: 510627 Room 802, No.19 Hai'an Road, Tianhe District, Guangzhou City, Guangdong Province Applicant before: GUANGDONG LEJIANG BIOTECHNOLOGY Co.,Ltd. |
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Effective date of registration: 20230417 Address after: 414000 North side of Zhenghong Road, Tuishanzui Community, Quyuan Management District, Yingtian Town, Yueyang City, Hunan Province Patentee after: Hunan Zhenghong Lide Feed Co.,Ltd. Address before: 570000 room 505, Building 29, General Hospital of state farms, 48 baishuitang Road, Longhua District, Haikou City, Hainan Province Patentee before: Guo Shengcheng |