CN108056104A - A kind of willow compound for inhibiting fall webworms growth and its application - Google Patents
A kind of willow compound for inhibiting fall webworms growth and its application Download PDFInfo
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- CN108056104A CN108056104A CN201610968013.7A CN201610968013A CN108056104A CN 108056104 A CN108056104 A CN 108056104A CN 201610968013 A CN201610968013 A CN 201610968013A CN 108056104 A CN108056104 A CN 108056104A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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Abstract
The present invention relates to a kind of willow compound for inhibiting fall webworms growth and its applications, belong to pest-resistant willow assistant breeding and fall webworms Prevention Technique field.The present invention is by more comprehensively analyzing pest-resistant and sense worm material compound difference, with reference to correlation analysis, identify stigmasterol be willow to one of main compound of fall webworms resistance, there is apparent inhibitory action to the development of fall webworms larva.The compound can be used as metabolic marker object, for the assisted Selection of pest-resistant Poplar Breeding；Pesticide composition is can also be used as, is prevented for fall webworms, is of great significance for effective control of willow breeding for pest resistance and fall webworms.Stigmasterol assisted Selection with traditional breeding method is effectively combined, breeding time can be shortened, accelerates breeding speed, improves willow breeding for pest resistance efficiency.
Description
Technical field
The present invention relates to a kind of willow compounds for inhibiting fall webworms growth and development and the compound to prevent in fall webworms
Application in controlling.More particularly to application of the stigmasterol in pest-resistant Poplar Breeding and fall webworms prevention, it is auxiliary to belong to pest-resistant willow
Help breeding and fall webworms Prevention Technique field.
Technical background
Willow (Populus L) be China three Norths shelter-forest, farm field and forest network and construction of turning the four sides green chief species and
The important source material of the industrial cut stocks such as pulpwood, wood-based plate, plays an important role in economic construction.China's Poplar Cultivation area compared with
Extensively, but due to the influence of the factors such as environment, weather, Poplar Plantation cultivation is faced with serious pest problem.
Fall webworms are a kind of important international quarantine pest insects, with feeding habits are miscellaneous, breeding amount is big, adaptable, propagation
The features such as approach is wide.Many provinces and cities such as Hebei, Tianjin, Shandong have been reached, have been given since China Dandong is found for the first time from 1979
China's production of forestry and ecological safety cause heavy losses and potential threat.(the national U.S. is white within 2004 by Zhao Tie treasure et al.
Non-economic loss assessment [J] the Problems of Forestry Economics in moth epidemic-stricken area, 2006,26 (4)：Caused by 321-326) assessing fall webworms
National non-economic loss total amount adds up to 140.35 hundred million yuan.Due to the commerial growing of willow, the white moth of current American has become danger
One of important leafeating insect of evil China willow.
In the common preventive measures of fall webworms, chemical prevention is quick, efficient, but environmental pollution is larger, no
Beneficial to sustainable development.Biological control environmental pollution is small, effectively Protect natural enemies, performance persistently can control calamity effect, but desinsection
Effect is slower, using the rapid purpose for forcing down insect population cannot be fully achieved under high insect density.Physical control is simple, practical,
But have the shortcomings that time-consuming and laborious, efficiency is low, it is thorough to be not easy.
Compound is the terminal executor of plant anti-insect reaction, and even more important effect is played during pest-resistant.It plants
Insect is even killed in the growth and development that object Insecticide can inhibit insect, so as to reduce harm of the insect to host.It utilizes
Plant Pesticidal compound itself is prevented, and is expected to realize to target pest insecticide efficiency height, and to non-target insects and environment
Influence smaller purpose.
At present, it has been reported that the willow Insecticide for leafeating insect mainly have, to gypsymoth (Lymantria
Dispar L.) the inhibited chlorogenic acid of growth and development (chlorogenic acid), to chrysomelid performance resistance effect
Condesed tannins (condensed tannins) and to tent caterpillar (Malacosoma disstria) feeding have inhibit
Tremulacin (tremulacin) of effect etc..
But there is presently no the reports of the willow Insecticide for fall webworms.Due to the physiology of different insects
And feeding characteristic has differences, it may be different to its effective plant anti-insect compound.In addition, the Insecticide kind of plant
Class is various, and structure is totally different, it is necessary to carry out correlative study, to excavate more willow Insecticides.
Therefore, study has the willow compound killed or develop inhibitory action for fall webworms, for fall webworms
Effective control and the cultivation of willow pest-resistant cultivar be of great significance.
The content of the invention
An object of the present invention is to provide a kind of willowization for fall webworms with poisoning or development inhibitory action
Object is closed, effectively to control the generation of fall webworms.
The purpose of foregoing invention of the present invention, reaches by the following technical programs：
A kind of application of compound for inhibiting fall webworms growth and development in the product for preventing fall webworms is prepared,
The compound is stigmasterol.
Preferably, the stigmasterol is as chemical pesticide main ingredient, for the chemical prevention of fall webworms.
There is the willowization killed or develop inhibitory action for fall webworms it is a further object of the present invention to provide a kind of
Object is closed, uses it for the pest-resistant assistant breeding of willow, breeding time can be shortened, accelerates breeding speed, improves willow breeding for pest resistance
Efficiency.
The purpose of foregoing invention of the present invention, reaches by the following technical programs：
A kind of application of compound for inhibiting fall webworms growth and development in the pest-resistant assistant breeding of willow, the compound
For stigmasterol.
The present invention inhibition fall webworms growth and development compound through the following steps that identification：
(1) insect resistace measures
Fall webworms insect resistace measure is carried out to Poplar Varieties first, filters out pest-resistant Poplar Varieties and sense worm willow product
Kind；
(2) metabonomic analysis
The material of pest-resistant Poplar Varieties and sense worm Poplar Varieties is ground under freezing conditions, extracts total metabolism respectively
Object performs the derivatization after dry, and carries out GC-TOF/MS measure to compound；
(3) identification of difference compound
Compared by deconvolution, peak area extraction, mass spectrum and etc., it obtains in pest-resistant Poplar Varieties and sense worm Poplar Varieties
The composition and relative amount of compound are analyzed by one-dimensional and multidimensional statistics, are screened pest-resistant willow and are felt the difference between worm willow
Iso compounds, and compound relative amount and larvae development are gone through into phase progress correlation analysis, it identifies and fall webworms resistance phase
The high compound of closing property is the stigmasterol in poplar leaf.
After the step (3), it can also increase following steps：
(4) verification of difference compound
Using GC/MS and external standard method, to pest-resistant No. 36 poplars of Poplar Varieties and No. 50 poplars, 2025 He of sense worm Poplar Varieties middle forest
The content of stigmasterol in populus blade is measured, and demonstrates metabonomic analysis as a result, stigmasterol in i.e. pest-resistant material
Content apparently higher than sense worm material, content and the larvae development of stigmasterol go through the phase and are proportionate.
Poplar Varieties described in the step (1) are 14, and the pest-resistant cultivar filtered out is No. 36 poplar (Populus
Deltoides cv. ' 2KEN8 ') and No. 50 poplars (P.deltoides cv. ' 55/56 '), the sense worm kind filtered out is middle forest
2025 (P.deltoides cv. ' Zhonglin 2025 ') and populus (P.deltoides cv. ' Nankang ').
It is that (GC is Agilent6890 gas phases to GC-TOF/MS to compound determination method therefor described in the step (2)
Chromatography, series connection LECO companies Pegasus III mass spectrums).
Advantageous effect
The present invention is by more comprehensively analyzing pest-resistant and sense worm material compound difference, with reference to correlation analysis, identification
It is willow to one of main compound of fall webworms resistance to go out stigmasterol.Man-made feeds addition experiment finds that stigmasterol is to U.S.
The development of the white moth larvae of state has apparent inhibitory action really.The compound can be used as metabolic marker object, for pest-resistant poplar
Assisted Selection can also be used as pesticide composition, be prevented for fall webworms, for the effective of willow breeding for pest resistance and fall webworms
Control is of great significance.
Stigmasterol assisted Selection with traditional breeding method is effectively combined, breeding time can be shortened, accelerates breeding speed,
Improve willow breeding for pest resistance efficiency.
Below by the drawings and specific embodiments, the invention will be further described, but is not meant to protect the present invention
Protect the limitation of scope.
Description of the drawings
Fig. 1 is that the man-made feeds of addition stigmasterol go through fall webworms larvae development the influence of phase.
Specific embodiment
Embodiment 1
The identification method of compound associated with the anti-fall webworms character of willow, its step are as follows：
(1) insect resistace measures
By Poplar Varieties (the see the table below 1) plantation cultivated extensively in 14 productions to same nursery by the way of cuttage
In, carry out fall webworms insect resistace measure when growing to 2 years raw saplings.Poplar leaf is placed in ice chest, takes back insect culture
Between (25 ± 1 DEG C；14 it is small when illumination), be packed into insect blake bottle in (bottom footpath=12cm；Height 15cm).It will be at the beginning of 2 age of fall webworms
Larva is put into the blake bottle equipped with poplar leaf, and every bottle puts 30.Fresh blade was changed once every 3 days, feeds fall webworms
To pupating.Period, each instar larvae weight of statistics fall webworms, larvae development go through phase, percentage of pupation and the death rate.Each kind is set
3 repetitions.Statistical result showed, fall webworms larval mortality is not significantly different between surveyed kind, but larvae development
Index, percentage of pupation and pupa weight significant difference (see the table below 2).Judge Poplar Varieties to the worm according to the development index of fall webworms
Resistance level.Therefrom select more anti-fall webworms No. 36 poplars (Populus deltoides cv. ' 2KEN8 ') and No. 50 poplars
(P.deltoides cv. ' 55/56 ') relatively feels the (P.deltoides cv. ' Zhonglin of middle forest 2025 of fall webworms
2025 ') and populus (P.deltoides cv. ' Nankang ').
Table 1 is used for the Poplar Varieties of insect resistace test
| Variety name | Latin name | Source |
| No. 36 poplars | Populus deltoides cv.‘2KEN8’ | Forestry Institue, Chinese Inst.of Forestry Sciences preserves |
| No. 50 poplars | P.deltoides cv.‘55/56’ | Forestry Institue, Chinese Inst.of Forestry Sciences preserves |
| Nan Yang | P.deltoides cv.‘Nanyang’ | Forestry Institue, Chinese Inst.of Forestry Sciences cultivates |
| Middle forest 2025 | P.deltoides cv.‘Zhonglin 2025’ | Forestry Institue, Chinese Inst.of Forestry Sciences cultivates |
| Red red poplar | P.deltoides cv.‘Danhong’ | Forestry Institue, Chinese Inst.of Forestry Sciences cultivates |
| Quan Hongyang | P.deltoides cv.‘Quanhong’ | Forestry scientific research institute of Henan Province cultivates |
| Populus | P.deltoides cv. ' Nankang 3 ' | Forestry Institue, Chinese Inst.of Forestry Sciences cultivates |
| Populus nigra | Populus nigra L. | Forestry Institue, Chinese Inst.of Forestry Sciences preserves |
| 107 poplars | P.×euramericana cv.‘Neva’ | Forestry Institue, Chinese Inst.of Forestry Sciences preserves |
| 108 poplars | P.×euramericana cv.‘Guariento’ | Forestry Institue, Chinese Inst.of Forestry Sciences preserves |
| 2012 poplar of European-American Poplar | P.×euramericana cv.‘Por’ | Forestry Institue, Chinese Inst.of Forestry Sciences preserves |
| 109 poplars | P.deltoides×P.alba cv.‘Mincio’ | Forestry Institue, Chinese Inst.of Forestry Sciences preserves |
| Gloomy extra large No. 1 | P.deltoides×P.cathayana cv.‘Senhai 1’ | Forestry Institue, Chinese Inst.of Forestry Sciences cultivates |
| Gloomy extra large No. 2 | P.deltoides×P.cathayana cv.‘Senhai 2’ | Forestry Institue, Chinese Inst.of Forestry Sciences cultivates |
The fall webworms larvae development indicator-specific statistics of 2 feeding difference Poplar Varieties of table
Note：It is entirely different with shoulder mark lowercase in column data, represent significant difference (P ＜ 0.05)
(2) metabonomic analysis
By No. 36 poplars (Populus deltoides cv. ' 2KEN8 ') of more anti-fall webworms and No. 50 poplars
(P.deltoides cv. ' 55/56 ') relatively feels the (P.deltoides cv. ' Zhonglin of middle forest 2025 of fall webworms
2025 ') and after the blade 100mg of populus (P.deltoides cv. ' Nankang ') grinds in liquid nitrogen, add in 2mL and shift to an earlier date
It is stored in -20 DEG C of 1 (methanol of extraction mixed solution：Chloroform：Water=2.5：1：1(v：v：V) in), and the nonadecane of 10 μ L is added in
The ribitol (0.2mg/mL) of sour (2mg/mL) and 50 μ L are placed respectively as lipid phase and methanol/water phase internal standard on shaking table
220rpm is sufficiently mixed 30min, and then 11000g/min centrifuges 10min, and transfer supernatant obtains the into clean 10ml centrifuge tubes
Supernatant；2 (the methanol of solution that 1mL is stored in -20 DEG C is added in precipitation：Chloroform=1：1(v：V)), place on shaking table
220rpm is sufficiently mixed 30min, 11000g/min centrifugations 10min；Collect second of supernatant, and the supernatant collected with first time
Liquid merges, and adds in the deionized water after 500 μ L sterilizings, 5000g/min centrifugations 3min；Then, upper strata methanol/water is mixed respectively
Liquid and lower floor's chloroform phase solution are transferred in clean centrifuge tube；Methanol/water phase solution is dried with vacuum freeze drier, chloroform
Phase solution is dried up with nitrogen；Dried sample put -80 DEG C it is spare or directly perform the derivatization；Add in 50 μ L pyridinium dissolutions
Methoxy semicarbazide hydrochloride (20mg/ml), seal, be vortexed, of short duration centrifugation；30 DEG C of warm bath 90min；Add in 80 μ L silylating reagents
N- methyl-N- (trimethyl silane)-trifluoroacetamide (N-methyl-N- (trimethylsilyl)-
Trifluoroacetamide, MSTFA), 37 DEG C of warm bath 30min；After sample after derivatization is placed at room temperature for 2h, GC- is carried out
TOF/MS is detected；GC is Agilent6890 gas-chromatographies, LECO companies Pegasus III mass spectrums of connecting；Gas chromatographic column is
DB-5ms (J＆W Scientific, Folsom, CA, USA), carrier gas are helium, flow velocity 1.5ml/min；Chloroform mutually use regardless of
Stream mode, starting column temperature are 80 DEG C, maintain 4min, are then raised to 330 DEG C with 5 DEG C/min speed, keep 5min；Methanol/water phase
Temperature program is to originate 80 DEG C of column temperature, maintains 4min, with 4 DEG C/min speed to 260 DEG C, keeps 1min, then with 20 DEG C/min speed
It spends to 320 DEG C, keeps 3min.Using 1:10 split ratios；250 DEG C of injector temperature, 200 DEG C of ion source temperature, spectrogram delay are adopted
Collect 5min, matter lotus m/z detection ranges 70~600, scan 20 spectrograms each second, detect 1700 volts of voltage, EI electron bombardment energy
Measure 70eV.
(3) screening of difference compound
The pre-treatments such as peak smoothing denoising, baseline correction, peak alignment are carried out to mass spectrometric data using ChromaTOF softwares, and
Mass spectrometric data is extracted after deconvolution；Information in Mass Spectra is submitted to National Institute of Standards and Technology Zhi Pu Ku ﹙
National Institute of Standards and Technology, NIST) metabolin database, pass through Information in Mass Spectra
Compare, identify metabolin, resisted/felt the metabolome of kind into and its relative amount；Using SIMCA-P+ softwares to mass spectrum
Data carry out Model checking analysis, go out the pest-resistant difference compound between sense worm kind based on PLS-DA model discriminations；It utilizes
17.0 software statistics of SPSS are analyzed, in the horizontal further verification difference compound of one-dimensional statistics；By difference metabolin and insect resistace
Shape larvae development goes through phase and larval weight and carries out Pearson correlation analyses, identify stigmasterol be with pest-resistant characters correlation compared with
One of high compound；
(4) verification of difference compound
Absolute quantitation is carried out to stigmasterol content in pest-resistant and sense worm kind blade using external standard method；Weigh the beans steroid of purchase
Alcohol standard items 1.2mg is added in 10mL volumetric flasks, is shaken up and be settled to graduation mark, mother liquor is made；Take appropriate mother liquor, add ethyl alcohol by
Grade is diluted to 1/10,1/2,1,5,10 times of mother liquid concentration, is configured to the gradient solution of stigmasterol；Utilize Agilent gas chromatography mass spectrometry
Instrument (GC-MS, 6890N-5973N) carries out Specification Curve of Increasing and sample size measures；Gas chromatographic column is DB-5ms (J＆W
Scientific, Folsom, CA, USA), carrier gas is helium, flow velocity 1.5mL/min；Using 1:10 shunt modes originate column temperature
80 DEG C, 4min is maintained, with 4 DEG C/min speed to 260 DEG C, keeps 1min, then with 20 DEG C/min speed to 320 DEG C, is kept
3min.；250 DEG C of injector temperature, 200 DEG C of ion source temperature, spectrogram delay acquisition 5min, matter lotus m/z detection ranges 70~
600, each second scans 20 spectrograms, detects 1500 volts of voltage, EI electron bombardment energy 70eV；With the peak area (A) of stigmasterol
Corresponding concentration (C) carries out linear regression analysis；The result shows that standard curve is in investigation quality concentration range (0.0012mg/mL
~1.2mg/mL) in a linear relationship, related coefficient 0.9992；Using this method to beans steroid in pest-resistant and sense worm kind blade
Alcohol content is determined；Respectively with 0.5 times, 1 times and 1.5 times of the survey content progress rate of recovery calculating, the rate of recovery exists
Between 80.4% to 94.9%.Under the same conditions, 6 parallel testings are carried out to same sample, it is as a result as shown in table 3 below, 36
Number poplar (Populus deltoides cv. ' 2KEN8 '), No. 50 poplars (P.deltoides cv. ' 55/56 '), middle forest 2025
(P.deltoides cv. ' Zhonglin 2025 '), stigmasterol is flat in populus (P.deltoides cv. ' Nankang ')
Equal content is respectively 0.35mg/g, 0.25mg/g, 0.07mg/g and 0.03mg/g.The content of the substance is bright in pest-resistant Poplar Varieties
Aobvious changes of contents trend is consistent with metabolism group higher than sense worm Poplar Varieties, and going through the phase with larvae development is proportionate.
Stigmasterol content and fall webworms Resistant index correlation analysis in 3 poplar leaf of table
Note：* is significantly correlated in 0.01 level
Embodiment 2
Application of the stigmasterol compound in the product for preventing fall webworms is prepared
Using ethyl alcohol as solvent, prepare stigmasterol solution, concentration be respectively 0.1 times of No. 36 poplar blade contents of pest-resistant cultivar and
1 times, i.e. 35 μ g/ml and 350 μ g/ml.Fall webworms man-made feeds are prepared, and are poured into foster worm cup.It is molten to draw 200 μ L stigmasterol
Liquid is spread evenly across man-made feeds surface, is subsequently placed in ventilating kitchen clean to ethyl alcohol volatilization.Each insect raising cup is placed about
Then 100 newly hatched larvaes are raised (25 ± 1 DEG C in insect is raised；14 it is small when illumination).Treat insect growth to 3 ages
Just, the insect head number raised in cup is reduced to 30 at random, it is to be grown to being distributed into 5 every bottle after 4 ages.One was changed every 2 days
The secondary man-made feeds added with stigmasterol, feeding fall webworms are to pupating.Each concentration sets 4 repetitions.The period statistics U.S. is white
Moth larvae development duration.The results are shown in Figure 1, is that the stigmasterol of the feeding present invention adds man-made feeds to fall webworms larva hair
Educate the influence for the phase of going through.Wherein, solvent control is the ethyl alcohol without stigmasterol, and processing 1 is that stigmasterol addition concentration is 35 μ g/ml；
Processing 2 is that stigmasterol addition concentration is 350 μ g/ml.The larvae development of control and various concentration processing goes through the phase from Fig. 1, can be with
See, the larvae development of feeding processing 2 (stigmasterol that addition concentration is 350 μ g/ml) and feeding solvent control goes through the difference of phase
Reach the pole level of signifiance (* * represent P ＜ 0.01).
The stigmasterol (stigmasterol that addition concentration is 350 μ g/ml) of 1 times of concentration of addition can significantly extend larvae development and go through
Phase.Compared with solvent control, in the case where 1 times of concentration is added, larvae development goes through phase extension 5.2 days.
Embodiment 3
Application of the stigmasterol in pest-resistant willow assistant breeding
It is the validity and accuracy of guarantee test in willow pest-resistant cultivar Breeding Process, insect resistace test is typically based on 1
Year or more trees carry out, and to be observed in test process at least one insect from generation to generation, the cycle is long, time-consuming and laborious.Compound in plant
Content determine that this research is verified by metabonomic analysis, correlation analysis and insect resistace, is found in poplar leaf by genotype
The content of stigmasterol is proportionate with fall webworms resistance, therefore, can predict willow using stigmasterol as metabolic marker object
To the resistance level of fall webworms.
Using stigmasterol carry out the pest-resistant assisted Selection of willow implementing procedure be：Selection and breeding are detected by chromatography or mass spectrograph
The content of stigmasterol in the blade of body, compares the height of the content of material in Different Individual, and then speculates it to fall webworms
Relevant antagonism is horizontal；Willow breeding for pest resistance assisted Selection is carried out using stigmasterol as metabolic marker object, Seedling selection can be allowed,
Save human and material resources and the financial resources that cultivating process is wasted；And the implementation of Seedling selection, more groups can also allow for receive
Among the object for entering to study selection, more extensive and intensity bigger selection is realized；In addition, the insect resistace that individual carries out is evaluated logical
Often with there is destructiveness, individual later period growth and development is influenced；And assisted Selection is carried out using stigmasterol, a small amount of blade is only needed, individual
It can also continue to grow, so that breeder simultaneously carries out the breeding population selection of other characters；Therefore, it is stigmasterol is auxiliary
Selection is helped effectively to be combined with traditional breeding method, breeding time can be shortened, accelerates breeding speed, improves willow breeding for pest resistance effect
Rate.
The present invention is by more comprehensively analyzing pest-resistant and sense worm material compound difference, with reference to correlation analysis, identification
It is willow to one of main compound of fall webworms resistance to go out stigmasterol.Man-made feeds addition experiment finds that stigmasterol is to U.S.
The development of the white moth larvae of state has apparent inhibitory action really.The compound can be used as metabolic marker object, for pest-resistant poplar
Assisted Selection can also be used as pesticide composition, be prevented for fall webworms, for the effective of willow breeding for pest resistance and fall webworms
Control is of great significance.
Claims (4)
1. application of the stigmasterol in the product for preparing prevention fall webworms.
2. application of the stigmasterol according to claim 1 in the product for preparing prevention fall webworms, it is characterised in that：Institute
Stigmasterol is stated as chemical pesticide main ingredient, for the chemical prevention of fall webworms.
3. application of the stigmasterol in pest-resistant willow assistant breeding.
4. application of the stigmasterol according to claim 3 in pest-resistant willow assistant breeding, it is characterised in that：The beans steroid
Alcohol is used as the metabolic marker object in pest-resistant willow assistant breeding.
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| CN113068697A (en) * | 2021-04-07 | 2021-07-06 | 中国林业科学研究院森林生态环境与保护研究所 | Application of sinapine aldehyde glucoside in preparation of narrow-leaved Croton wax product and insect-resistant breeding |
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| 侯莹莹 等: "RP-HPLC同时测定杨树花和杨树叶中3个植物甾醇含量", 《药物分析杂志》 * |
| 程立超 等: "杨树树皮挥发物的超临界萃取及对青杨脊虎天牛行为的影响", 《林业科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113068697A (en) * | 2021-04-07 | 2021-07-06 | 中国林业科学研究院森林生态环境与保护研究所 | Application of sinapine aldehyde glucoside in preparation of narrow-leaved Croton wax product and insect-resistant breeding |
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| CN108056104B (en) | 2020-10-09 |
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