CN108051462A - A kind of Micro-CT scanning Multi-example preparation facilities and application method - Google Patents
A kind of Micro-CT scanning Multi-example preparation facilities and application method Download PDFInfo
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- CN108051462A CN108051462A CN201711281160.8A CN201711281160A CN108051462A CN 108051462 A CN108051462 A CN 108051462A CN 201711281160 A CN201711281160 A CN 201711281160A CN 108051462 A CN108051462 A CN 108051462A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N23/00—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00
- G01N23/02—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by transmitting the radiation through the material
- G01N23/04—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by transmitting the radiation through the material and forming images of the material
- G01N23/046—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by transmitting the radiation through the material and forming images of the material using tomography, e.g. computed tomography [CT]
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
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- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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Abstract
The present invention, which provides a kind of Micro-CT scanning Multi-example preparation facilities and method, the more preparation facilities of Micro-CT scanning sample, to be included:Stent, sample support bar, coaxial protector, paraffin, compression bar.The sample combination body position of the multiple sample compositions of sample support bar correction is sleeved on using coaxial protector, ensure that sample does not exceed the extended line scope of sample support bar, ensures that rotating diameter during all samples test is no more than sample support shank diameter.Viscous paraffin is used as jointing material, can easily and fast be cemented multiple sample cohesives to together, then with sample support bar, more can easily be filtered this out in subsequent image processing, and is completed, sample is easy to remove, separate, and is not damaged.Equipment is simple, easy to operate, time saving and energy saving.
Description
Technical field
The present invention relates to a kind of sample preparation methods, and in particular to a kind of Micro-CT scanning Multi-example preparation facilities and user
Method.
Background technology
Micro-CT scanning(Micro-Computed Tomography), X-ray microfault photography is also referred to as, refers to space point
Resolution can reach the CT of lum or so.The main composition of Micro-CT scanning includes X radiographic sources, specimen rotating holder and high-resolution and visits
Survey three parts of device.X-ray source and detector are fixed, and sample rotates between X radiographic sources and detector, are produced by X radiographic sources
Raw continuous taper X beams penetrate sample to be tested, are imaged on X-ray detector.By rate at an angle to sample
Product are rotated, and obtain x-ray perspective view of the sample in different angle.By corresponding computer software, by each angle
Projected image be reconstructed, be reduced into the analyzable 3D rendering in computer.In order to reach higher resolution ratio, when rotating
Step-length it is smaller, the time for exposure is longer, while in order to improve clarity and accuracy, each angle gathers the progress of multiple images
Image co-registration and correction, so the parameter testing of Micro-CT scanning and image acquisition time are all very long.In daily microscopic CT scanning point
During analysis, if only making a sample every time, scan efficiency can be made very low, frequently switch on ray and sample door, to the damage of instrument
Consumption is also bigger.So in order to save the time, raising testing efficiency often will be to several samples similar in X-ray absorption coefficient
It is put on a sample stage and is carried out at the same time test.Due to the limited area of the CCD or other detectors that are used for Image Acquisition, in order to
Limited(CCD)It observes within sweep of the eye as far as possible big(It is more)Sample volume(Quantity), it is necessary to as far as possible ensure sample to
The axle center set of instrument turntable.Since instrument turntable is relatively large in diameter, sample needs to pass through the support concentric with turntable
Bar connects turntable.Therefore, sample must try one's best the center line set to sample support bar, and rotating diameter is straight no more than supporting rod
Footpath.Due to current Micro-CT scanning negligible amounts, sample preparation device is few, and conventional sample preparation means easily make sample beyond support shank diameter model
It encloses, success rate is not high, it is impossible to meet the Multi-example rapid prototyping requirement of Micro-CT scanning.
The content of the invention
It is an object of the invention to overcome the shortage of prior art, a kind of Micro-CT scanning Multi-example preparation facilities and method are provided,
Rotary sample diameter is made to be no more than sample support shank diameter.
Detailed description of the invention
The present invention provides a kind of Micro-CT scanning Multi-example preparation facilities, which includes:Stent(6), sample support bar(1), coaxially
Protector(2), paraffin(3), compression bar(5).
The stent(6)For with level table, height adjuster(7)Four firm leg supports, have energy among table top
Enough through sample support bar(1)Hole, table top size will ensure coaxial protector of setting aside concerns completely.
The sample support bar(1)For cylindrical type, one end is connected with instrument turntable, and one end holds sample.Hold sample
One end is equipped with groove(8), length and diameter is according to instrument needs, long 100-200mm, a diameter of 1-10mm.Further, it is described
Bottom portion of groove for taper, conical section depth is 1-5mm, and upper end is cylinder, and cylindrical part depth is 1-5mm.
The coaxial protector(2)It for openable and closable thick-walled tube, is made of two semi-circular tubes, apposition position has positioning to fill
It puts, round tube inner wall is smooth, and it is identical that internal diameter and supporting rod hold sample one end diameter, height 30-100mm, wall thickness 5-10mm, material
For polytetrafluoroethylene (PTFE).
The paraffin(3)It, can be by sample and sample support bar with certain viscosity and plasticity(1)It is fixed(It is viscous)
Together, it will not spring back sample after external force revocation.
The compression bar(5)For a cylinder, diameter is slightly less than coaxial protector(2)Internal diameter, can be in coaxial protector
(2)It inside moves freely, material is polytetrafluoroethylene (PTFE).
Using apparatus above, the present invention provides a kind of Micro-CT scanning Multi-example preparation method, is as follows:
1. differentiating Micro-CT scanning test needs according to certain, each sample is processed into suitable size, the comparable rectangular-shape of height
(For unmanageable sample or other shapes).The several sample both ends for needing to test are stained with appropriate paraffin, then
They are densely concentrated together, an assembly will be bonded as by the paraffin at both ends, make the sample being brought together as far as possible
Product assembly(4)Diameter is minimum.
2. to ensure that high-resolution and larger effective range of observation, selection can accommodate institute's sample assembly(4)Before
Put as far as possible thin supporting rod.By sample support bar(1)It is put into stent vertically(6)In hole among table top, appropriate paraffin is taken(3)
Insert sample support bar(1)Top groove(8)It is interior, the 1/2 of groove is filled up, compacting.Then it is sample sets are fit(4)It is perpendicular
Directly it is put into groove(8)On interior paraffin, appropriate paraffin is continuously added, fills up groove, sample sets are fit(4)Bury groove in bottom
(8)In it is fixed.
3. by coaxial protector(2)It is separately placed on the stent table top of sample support bar both sides, is adjusted by support height
Device(7)Adjusting bracket(6)Highly, make sample sets fit(4)Top is less than coaxial protector(2)Top.
4. by coaxial protector(2)Be closed, be tightly sleeved on sample support bar, correcting sample assembly, make its without departing from
Sample support shank diameter scope.Then compression bar is used(5)Gently downward top pressure sample sets are fit(4), make sample sets fit(4)Position
It is more firm.
5. by coaxial protector(2)It is careful outwards to separate, expose sample support bar(2)It is fit with sample sets(4), use toothpick
It is etc. suitable instrument that sample sets are fit(4)The paraffin of bottom(3)To sample(4)Upper accumulation, sample sets are fit(4)It is securely solid
Surely sample support bar is arrived(1)On, it is noted that paraffin(1)It cannot exceed outside sample support bar.
6. again by coaxial protector(2)Close sample support bar(1)On, if previous step operation causes sample combination
Body(4)It beyond sample support shank diameter scope, is recalled to, coaxial protector is removed after standing 3-5 minutes(2), with toothpick etc.
Suitable instrument is fit by sample sets(4)The paraffin of bottom(3)Compacting.
7. step 6 is repeated, until removing coaxial protector(2)Afterwards, the combination of sample sets zoarium bottom and paraffin is unchanged,
Sample preparation finishes.
Advantageous effect
The present invention is sleeved on the sample combination body position of the multiple sample compositions of sample support bar correction using coaxial protector, ensure that
Sample does not exceed the extended line scope of sample support bar, ensures that rotating diameter during all samples test is no more than sample support
Shank diameter.Equipment is simple, easy to operate, time saving and energy saving.Made using this materials of low density being made of C, H element of viscous paraffin
For jointing material, multiple sample cohesives can be cemented to together, then with sample support bar easily and fast, it more can be in subsequent figure
It as easily being filtered this out in processing, and is completed, sample is easy to remove, separate, and is not damaged.
Description of the drawings
The Micro-CT scanning Multi-example preparation facilities vertical section structure schematic diagram of Fig. 1 present invention;
Fig. 2 is the vertical section structure schematic diagram of sample support bar top groove in the present invention.
Wherein, 1- sample supports bar, the coaxial protectors of 2-, 3- paraffin, 4- sample sets are fit, 5- compression bars, 6- stents, 7- branch
Frame height adjuster, 8- sample support bar grooves.
Specific embodiment
With reference to embodiment and Figure of description, the present invention is further elaborated, but institute's protection domain of the present invention is not limited to
This.
The embodiment of the present invention provides a kind of Micro-CT scanning Multi-example preparation facilities, which includes:Stent(6), sample support
Bar(1), coaxial protector(2), paraffin(3), compression bar(5).
The stent(6)For with level table, height adjuster(7)Four firm leg supports, have energy among table top
Enough through sample support bar(1)Hole, table top size will ensure coaxial protector of setting aside concerns completely.
The sample support bar(1)For cylindrical type, with can be connected with Micro-CT scanning turntable, one end holds sample for one end, contains
Setting-out product one end is equipped with groove(8), long 120mm, a diameter of 8mm.Further, the bottom portion of groove be taper, conical section
Depth is 2mm, and upper end is cylinder, and cylindrical part depth is 2mm.
The coaxial protector(2)It for openable and closable thick-walled tube, is made of two semi-circular tubes, apposition position has positioning to fill
It puts, round tube inner wall is smooth, internal diameter 8mm, height 50mm, wall thickness 10mm, and material is polytetrafluoroethylene (PTFE).
The paraffin(3)With certain viscosity and plasticity, sample can be sticked together, then with sample support bar
(1)It is fixed(It is viscous)Together, it will not spring back sample after external force revocation.
The compression bar(5)For a cylinder, diameter 7mm can be in coaxial protector(2)It inside moves freely, material is poly-
Tetrafluoroethene.
Using apparatus above, the present invention provides a kind of Micro-CT scanning Multi-example preparation method, is as follows:
1. removing from office Micro-CT scanning test needs according to different process ox-hide, 4 leather samples are processed into the length of 3mm*3mm*8mm sizes
Cube shape.After 4 sample both ends are stained with appropriate paraffin, it is densely concentrated together, will be bonded as by the paraffin at both ends
One assembly makes the sample sets being brought together fit as far as possible(4)Diameter is minimum.
2. to ensure high-resolution and larger effective range of observation, the sample strut of above-mentioned 8mm diameters is selected.By sample
Product supporting rod(1)It is put into stent vertically(6)In hole among table top, appropriate paraffin is taken(3)Insert sample support bar(1)Top
Groove(8)It is interior, the 1/2 of groove is filled up, compacting.Then it is sample sets are fit(4)It is put into groove vertically(8)Interior paraffin
On, appropriate paraffin is continuously added, fills up groove, sample sets are fit(4)Bury groove in bottom(8)In it is fixed.
3. by coaxial protector(2)It is separately placed on the stent table top of sample support bar both sides, is adjusted by support height
Device(7)Adjusting bracket(6)Highly, make sample sets fit(4)Top is less than coaxial protector(2)Top.
4. by coaxial protector(2)Be closed, be tightly sleeved on sample support bar, correcting sample assembly, make its without departing from
Sample support shank diameter scope.Then compression bar is used(5)Gently downward top pressure sample sets are fit(4), make sample sets fit(4)Position
It is more firm.
5. by coaxial protector(2)It is careful outwards to separate, expose sample support bar(2)It is fit with sample sets(4), use toothpick
It is etc. suitable instrument that sample sets are fit(4)The paraffin of bottom(3)To sample(4)Upper accumulation, sample sets are fit(4)It is securely solid
Surely sample support bar is arrived(1)On, it is noted that paraffin(1)It cannot exceed outside sample support bar.
6. again by coaxial protector(2)Close sample support bar(1)On, if previous step operation causes sample combination
Body(4)It beyond sample support shank diameter scope, is recalled to, coaxial protector is removed after standing 3-5 minutes(2), with toothpick etc.
Suitable instrument is fit by sample sets(4)The paraffin of bottom(3)Compacting.
7. step 6 is repeated, until removing coaxial protector(2)Afterwards, the combination of sample sets zoarium bottom and paraffin is unchanged,
Sample preparation finishes.
Above example is merely to illustrate the present invention and is used rather than limitation of the present invention, person skilled in the relevant technique,
In the case of not departing from the spirit and scope of the present invention, various conversion or modification, therefore all equivalent technologies can also be made
Scheme should also belong to scope of the invention, should be limited by each claim.
Claims (7)
- A kind of 1. Micro-CT scanning sample Multi-example preparation facilities, which is characterized in that the Micro-CT scanning sample Multi-example preparation facilities bag It includes:Stent(6), sample support bar(1), coaxial protector(2), paraffin(3), compression bar(5).
- A kind of 2. Micro-CT scanning sample Multi-example preparation facilities according to claim 1, it is characterised in that the stent(6)For With level table, height adjuster(7)Four firm leg supports, sample support bar can be passed through by having among table top(1)'s Hole, table top size will ensure coaxial protector of setting aside concerns completely.
- A kind of 3. Micro-CT scanning sample Multi-example preparation facilities according to claim 1, it is characterised in that the sample support Bar(1)For cylindrical type, one end is connected with instrument turntable, and one end holds sample, holds sample one end equipped with groove(8), length Degree and diameter are according to instrument needs, long 100-200mm, a diameter of 1-10mm;Further, the bottom portion of groove is taper, is bored Shape partial depth is 1-5mm, and upper end is cylinder, and cylindrical part depth is 1-5mm.
- A kind of 4. Micro-CT scanning sample Multi-example preparation facilities according to claim 1, it is characterised in that the coaxial protection Device(2)For openable and closable thick-walled tube, be made of two semi-circular tubes, apposition position has positioner, and round tube inner wall is smooth, internal diameter with Supporting rod holds that sample one end diameter is identical, and height 30-100mm, wall thickness 5-10mm, material is polytetrafluoroethylene (PTFE).
- A kind of 5. Micro-CT scanning sample Multi-example preparation facilities according to claim 1, it is characterised in that the paraffin(3)Tool There are certain viscosity and plasticity, it can be by sample and sample support bar(1)It is fixed(It is viscous)Together, will not make after external force revocation Sample is sprung back.
- A kind of 6. Micro-CT scanning sample Multi-example preparation facilities according to claim 1, it is characterised in that the compression bar(5)For One cylinder, diameter are slightly less than coaxial protector(2)Internal diameter, can be in coaxial protector(2)It inside moves freely, material is poly- Tetrafluoroethene.
- 7. a kind of Micro-CT scanning Multi-example preparation method, which is characterized in that using the Micro-CT scanning any one of claim 1-6 Multi-example preparation facilities carries out sample preparation, and the Micro-CT scanning Multi-example preparation method comprises the following steps:Step 1. differentiates Micro-CT scanning test needs according to certain, and it is comparable rectangular that each sample is processed into suitable size, height Body shape(For unmanageable sample or other shapes), it would be desirable to several sample both ends of test are stained with appropriate paraffin, Then they are densely concentrated together, an assembly will be bonded as by the paraffin at both ends, make to be brought together as far as possible Sample sets it is fit(4)Diameter is minimum;Step 2. can accommodate institute's sample assembly for guarantee high-resolution and larger effective range of observation, selection(4)Before Put as far as possible thin supporting rod;By sample support bar(1)It is put into stent vertically(6)In hole among table top, appropriate paraffin is taken(3) Insert sample support bar(1)Top groove(8)It is interior, the 1/2 of groove is filled up, compacting;Then it is sample sets are fit(4)It is perpendicular Directly it is put into groove(8)On interior paraffin, appropriate paraffin is continuously added, fills up groove, sample sets are fit(4)Bury groove in bottom (8)In it is fixed;Step 3. is by coaxial protector(2)It is separately placed on the stent table top of sample support bar both sides, is adjusted by support height Device(7)Adjusting bracket(6)Highly, make sample sets fit(4)Top is less than coaxial protector(2)Top;Step 4. is by coaxial protector(2)Be closed, be tightly sleeved on sample support bar, correcting sample assembly, make its without departing from Sample support shank diameter scope;Then compression bar is used(5)Gently downward top pressure sample sets are fit(4), make sample sets fit(4)Position It is more firm;Step 5. is by coaxial protector(2)It is careful outwards to separate, expose sample support bar(2)It is fit with sample sets(4), use tooth The suitable instrument such as label is fit by sample sets(4)The paraffin of bottom(3)To sample(4)Upper accumulation, sample sets are fit(4)Securely Fixed to sample support bar(1)On, it is noted that paraffin(1)It cannot exceed outside sample support bar;Step 6. is again by coaxial protector(2)Close sample support bar(1)On, if previous step operation causes sample sets It is fit(4)It beyond sample support shank diameter scope, is recalled to, coaxial protector is removed after standing 3-5 minutes(2), use toothpick It is etc. suitable instrument that sample sets are fit(4)The paraffin of bottom(3)Compacting;Step 7. repeats step 6, until removing coaxial protector(2)Afterwards, the combination of sample sets zoarium bottom and paraffin is without change Change, sample preparation finishes.
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CN109253906A (en) * | 2018-11-05 | 2019-01-22 | 东北石油大学 | A kind of small core sample bonding preparation facilities and its bonding preparation method |
CN111929094A (en) * | 2019-05-13 | 2020-11-13 | 中国石油化工股份有限公司 | Sampling device and sampling method for nano CT test sample |
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