CN108048528A - It is simple and efficient real-time hereditary information acquisition methods and its application - Google Patents
It is simple and efficient real-time hereditary information acquisition methods and its application Download PDFInfo
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Abstract
The present invention relates to one kind to be simple and efficient real-time hereditary information acquisition methods and its application, and hereditary information acquisition methods include tissue treatment successively, take the appropriate amount of sample of the tissue of agriculture and animal husbandry plant or animal, grind homogenate；Nucleic acid extraction carries out nucleic acid extraction using paramagnetic particle method；Target gene and/or the capture of SNP segments carry out denaturation treatment to the nucleic acid that extraction obtains, and then using specificity, extract chip for the variety development gene for the fidelity of being traced to the source, then capture extraction and enrichment are carried out to target gene and/or SNP segments；Single-molecule sequencing is sequenced the target gene and/or SNP segments of capture with nanometer pore single-molecule sequencing approach, obtains the hereditary information of detection sample；Interpretation of result.Hereditary information acquisition methods provided by the invention, can be simple and efficient the hereditary information for obtaining individual in real time, and whole process is completed with full-automatic " fool " pattern, and genetic test can also be realized so as to fulfill non-professional general practitioners/consumer.
Description
Technical field
The present invention relates to biological technical fields, and in particular to one kind be simple and efficient real-time hereditary information acquisition methods and its
Using.
Background technology
Gene is the DNA fragmentation for including all hereditary information of bion, is had with life, and is remained unchanged all the life, and
And this hereditary information is lain in the nearly all histoorgan of individual.Therefore the hereditary information of individual can be used in mark differentiation
Individual, for identifying certification individual identity information.Parent-offspring is widely used to using the comparison certification individual of gene genetic information
In identification.And in agro-ecology (crops, livestock and poultry and aquatic biological) industry and using agro-ecology product as the food of raw material
In the industrial fields such as industry, it can fruitfully be used to prevent fraud fake traffic using the hereditary information of bion, trace to the source guarantor
Very.
Using gene genetic information realization trace to the source fidelity need obtain trace to the source individual gene information.Gene letter is obtained at present
There are mainly two types of the methods for ceasing data：Genotyping and gene sequencing.Genotyping is mainly more using known mononucleotide
The base sequence design probe of state property (SNP) site flank, and probe is fixed on genetic chip.As the DNA of sample to be tested
Hybridization is found out with the probe sequence Complementary hybridization on genetic chip and by scanning fluorescence signal, can be identified on sample DNA
The genotype of these probe sites (SNP site).And gene sequencing is that base sequence measure is carried out to target dna, and carry out phase
Close analysis.Gene sequencing technology can substantially be divided into for four generations at present.
First generation Sanger chain termination method sequencing technologies, basic principle are when archaeal dna polymerase utilizes four kinds of deoxyribonucleosides
When triphosphoric acid (dNTP) synthesizes the complementary strand of DNA to be measured, chain is used as by using corresponding dideoxyribonucleoside triphosphate (ddNTP)
It terminates reagent competitive substitution deoxynucleotide and generates extension segment a series of of different sizes and an only poor nucleotide, finally lead to
It crosses polyacrylamide gel electrophoresis to distinguish these extension segments, reads the base sequence of DNA molecular.
Second generation large-scale parallel sequencing technology, mainly using " while synthesis while be sequenced " principle, use different colours
4 kinds of different dNTP of fluorescent marker, when archaeal dna polymerase synthesizes the complementary strand of DNA to be measured, often adding a kind of dNTP will release
Different fluorescence is released, according to the fluorescence signal of capture so as to obtaining the sequence information of DNA to be measured.
The real-time sequencing technologies of third generation single molecular fluorescence, using the SMRT technologies of Pacific Bioscience companies of the U.S. as
It represents, is the fluorophor for being marked with different emission spectrum respectively on 4 kinds of dNTP.When archaeal dna polymerase catalytic fluorometry mark
When dNTP is incorporated into synthesis chain, the fluorescence signal entrained by dNTP, which is detected, is converted into corresponding base sequence information.
Forth generation nanometer pore single-molecule sequencing approach, with Oxford Nanopore Technologies companies of Britain
MinION sequencing technologies are representative.Its basic principle is when DNA molecular or its composition base are passed through from a biological nano hole
It is out-of-date, it can be to being had an impact by the electric current of nano-pore.Different molecules by when to electric current generate influence have can distinguish
Difference, can identify that base (to) puts in order in gene using this species diversity.Nanometer pore single-molecule sequencing approach pair
During DNA sequencing, without DNA is marked, without PCR amplification is carried out, real-time implementation is to the independent of each DNA molecular
Sequencing.
Two methods of above-mentioned gene sequencing and Genotyping in addition to forth generation nanometer pore single-molecule sequencing approach, are obtaining
It obtains individual gene information data and generally requires the complex steps such as elution process, cloning procedure, PCR amplification process, at the same it is many
Technology needs that DNA is marked, and to use expensive fluorometric reagent and CCD camera.Therefore obtained using these methods a
The gene information data of body need the professional technique equipment of complex and expensive and require to be operated by professional technician.However agriculture and animal husbandry
The gene of industry bion, which is traced to the source, requires the acquisition of gene data under daily production, living condition, by layman with
The pattern of " fool " is it is achieved that inexpensive simultaneously, high efficiency, it is therefore desirable to which a kind of easy, real-time, inexpensive method is come
The gene data for obtaining individual is realized.
The content of the invention
For in the prior art the defects of, present invention aims at provide one kind and to be simple and efficient real-time hereditary information to obtain
Method and its application, can simply, efficiently, automation, obtain in real time individual hereditary information, whole process is with full-automation
" fool " pattern complete, so as to fulfill non-professional general practitioners/consumer can also realize genetic test.
To achieve the above object, technical solution provided by the invention is：
Be simple and efficient real-time hereditary information acquisition methods the present invention provides one kind, method include successively tissue treatment,
Nucleic acid extraction, target gene and/or the capture of SNP segments, single-molecule sequencing and interpretation of result step.
Preferably, tissue treatment specifically includes：The appropriate amount of sample of the tissue of agriculture and animal husbandry plant or animal is taken, grinds homogenate.
Preferably, nucleic acid extraction specifically includes：Nucleic acid extraction is carried out using paramagnetic particle method, utilizes the specificity of magnetic bead and nucleic acid
Nucleic acid is obtained with reference to extraction.
Preferably, target gene and/or the capture of SNP segments specifically include：Denaturation treatment is carried out to the nucleic acid that extraction obtains,
Then using specificity, chip is extracted for the variety development gene for the fidelity of being traced to the source, then to target gene and/or SNP pieces
Duan Jinhang captures extraction and enrichment.
Preferably, gene extraction chip is to hybridize matched principle according to the kind and probe of the fidelity of being traced to the source to be set
It counts and is prepared.
Preferably, single-molecule sequencing specifically includes：Using nanometer pore single-molecule sequencing approach, to the target gene of capture
And/or SNP segments are sequenced, and obtain the hereditary information of detection sample.
Preferably, interpretation of result specifically includes：The hereditary information that the hereditary information obtained and gene label are marked
Verification is compared, so as to fulfill fidelity of tracing to the source.
The present invention also protection is simple and efficient real-time hereditary information acquisition methods and is carried out to the product containing hereditary information
Trace the application in source.
Preferably, the product containing hereditary information be selected from agro-ecology, using agro-ecology as the food of raw material and with agricultural
Biology is the one or more in the drug of raw material.
Preferably, one or more of the agro-ecology in crops, livestock and poultry and aquatic biological.
The main application of one of simple inheritance information acquisition method set forth in the present invention, is to serve gene label skill
Art produces and sells field and using some farming and animal husbandry products as the food processing and sale of raw material sources for farming and animal husbandry product
The fidelity of tracing to the source in field.In farming and animal husbandry field breeding variety be used for farming and animal husbandry product production when, can to educated breeding into
Row gene sequencing or Genotyping are used to make gene label so as to obtain the feature hereditary information of educated breeding.While this
Feature hereditary information is also used to design in the realization of simple inheritance information acquisition method the capture probe of gene extraction chip.When
Using simple inheritance information acquisition method cooperation Gene-tagging technology serve a certain agriculture and animal husbandry kind trace to the source fidelity when, pass through base
Because of the identification information of label, corresponding gene extraction chip or design probe can be selected to prepare corresponding gene extraction chip.
Then handle kind individual sample tissue and extract nucleic acid, combined capture target dna with gene extraction chip hybridization afterwards.Finally
Results of hybridization is eluted and carries out single-molecule sequencing, shows that detection individual belongs to traced to the source kind if sequencing data is obtained, together
When the individual inheritance information that can be identified with gene label of sequencing data done and further compare analysis.If there is no base sequence
Column signal generation then shows that detection individual is not belonging to traced to the source kind.
Simple inheritance information acquisition method set forth in the present invention, can based on forth generation nanometer pore single-molecule sequencing approach
With simple, efficient, automation, the hereditary information for obtaining individual in real time.Forth generation nanometer pore single-molecule sequencing approach, with Britain
The MinION sequencing technologies of Oxford Nanopore Technologies companies are representative.Its basic principle is to work as DNA molecular
Or its composition base is from a biological nano hole when passing through, it can be to being had an impact by the electric current of nano-pore.Different points
Son by when to electric current generate influence have diacritic difference, can identify in gene base (to) using this species diversity
Put in order.When nanometer pore single-molecule sequencing approach is to DNA sequencing, without DNA is marked, without progress PCR expansions
Increase, independent sequencing of the real-time implementation to each DNA molecular.
The fidelity identification of tracing to the source of agriculture and animal husbandry animals and plants product, can be divided into the identification of kind general character and individual character identifies two kinds.Product
Kind general character identification is the feature hereditary information that the kind detected belonging to individual is different from other kinds.When kind individual amount is more
When can not gather identification genes of individuals information one by one, then the gene information of sampling is often obtained by the way of sampling, is obtained at this time
What is obtained is the general character appraising datum information of kind.Individual character identification is the individual institute's characteristic feature hereditary information of detection, i.e., this
Body is different from the hereditary information data of other individuals of same kind, and most common individual character hereditary information is mononucleotide
Polymorphism (SNP).For SNP information, belong to data possessed by the Different Individual of same kind and differ, therefore SNP numbers
According to the genetic fingerprint profile information that can be used as the Different Individual for identifying same kind.When kind individual amount is less able to one by one
During cdna collection information, the characteristic hereditary information of each individual can be obtained one by one.It is directed to the variety plot for the fidelity of being traced to the source
Not in other kinds characteristic DNA probe (and SNP probes of kind individual) and specific designs gene extraction chip, be used for
The capture extraction of target dna.
Using simple inheritance information acquisition method, the animals and plants sample tissue to be identified is handled first, then
Utilize the nucleic acid extracted the methods of paramagnetic particle method in sample.Afterwards to extracted DNA fragmentation, denaturation is carries after single-stranded with gene
The probe hybridization of coring on piece combines.If containing the characterizing gene of identification of species is wanted (and/or to be identified in sample DNA
Kind individual SNP probe fragments base sequence), then just there is sample dna fragment to be combined with the probe hybridization on gene extraction chip.
After washing removes sample DNA, the DNA fragmentation combined with probe hybridization gene extracted on chip elutes, subsequently into nanometer
It is sequenced in the single-molecule sequencing device of hole, and its heredity in gene identities information database indicated with sample gene label
Verification is compared in information.If without the characterizing gene for want identification of species, (and the kind to be identified is individual in sample DNA
SNP probe fragments base sequence), then hybridize without the probe on sample dna fragment and gene extraction chip and combine.Washing removes
After sample DNA, there will not be the DNA fragmentation combined with probe hybridization to elute into nano-pore list on gene extraction chip
Molecule sequencing device, therefore will not have data-signal generation.
Technical solution provided by the invention has following advantageous effect：(1) simple inheritance information set forth in the present invention
The method of acquisition be based on forth generation nanometer pore single-molecule sequencing approach, can simply, efficiently, automation, obtain in real time it is a
The hereditary information of body；(2) method provided by the invention avoids the PCR amplification of DNA, fluorescence mark needed for conventional gene detection
Complex steps and expensive reagent and corresponding professional technique equipment and the professional technicians such as note；Using being explained according to the present invention
The detection device for the Technology design manufacture stated, it is only necessary to which operating personnel will detect the sample port of sample used device, own afterwards
Detecting step can be completed with full-automatic " fool " pattern, so as to fulfill non-professional general practitioners/consumption
Person can also realize genetic test.
The additional aspect and advantage of the present invention will be set forth in part in the description, and will partly become from the following description
It obtains substantially or is recognized by the practice of the present invention.
Description of the drawings
Fig. 1 is the schematic diagram for being simple and efficient real-time hereditary information acquisition methods in the embodiment of the present invention；
Fig. 2 in the embodiment of the present invention using be simple and efficient real-time hereditary information acquisition methods and coordinate gene label come
Realize the method schematic diagram of the fidelity of tracing to the source of group A.
Specific embodiment
Below in conjunction with the attached drawing in the embodiment of the present invention, the technical solution in the embodiment of the present invention is carried out clear, complete
Site preparation describes.The following examples are only intended to illustrate the technical solution of the present invention more clearly, therefore is intended only as example, without
It can be limited the scope of the invention with this.Experimental method in following embodiments is conventional side unless otherwise specified
Method.Test material used, is to be commercially available from routine biochemistry reagent shop unless otherwise specified in following embodiments.
Quantitative test in following embodiment, is respectively provided with three repeated experiments, and data are the average value or average value of three repeated experiments
± standard deviation.
As shown in Figure 1, the present invention provide one kind be simple and efficient real-time hereditary information acquisition methods, successively including tissue at
Reason, nucleic acid extraction, target gene and/or the capture of SNP segments, single-molecule sequencing and interpretation of result step, it is specific as follows.
Tissue treatment：The appropriate amount of sample of the tissue of agriculture and animal husbandry plant or animal is taken, quickly grinds homogenate.
Nucleic acid extraction：Nucleic acid extraction is carried out using paramagnetic particle method, is obtained using the specific binding extraction of magnetic bead and nucleic acid high
The nucleic acid of purity, high concentration.
Target gene and/or the capture of SNP segments：Denaturation treatment is carried out to the nucleic acid that extraction obtains, then utilizes specificity,
Matched principle is hybridized according to the kind for the fidelity of being traced to the source and probe and carries out gene extraction chip design and preparation, then using base
Because extraction chip carries out target gene and/or SNP segments capture extraction and enrichment.
Single-molecule sequencing：Using nanometer pore single-molecule sequencing approach, target gene and/or SNP segments to capture carry out
Sequencing obtains the hereditary information of detection sample.
Interpretation of result：Verification is compared with the hereditary information that gene label is marked in the hereditary information obtained, from
And realize fidelity of tracing to the source.
It is simple and efficient real-time hereditary information acquisition methods to provided by the invention with reference to specific embodiment and makees into one
Walk explanation.
Embodiment one
The present embodiment provides one kind to be simple and efficient real-time hereditary information acquisition methods, includes the following steps.
Tissue treatment：The appropriate amount of sample of kind A1, A2, A3, A4, A5, A6, B1 is taken respectively, quickly grinds homogenate.
Nucleic acid extraction：Paramagnetic particle method is respectively adopted and carries out nucleic acid extraction, is obtained using the specific binding extraction of magnetic bead and nucleic acid
Obtain high-purity, the nucleic acid of high concentration.
Target gene and the capture of SNP segments：Denaturation treatment is carried out to the nucleic acid that extraction obtains respectively, then using special
Property, matched principle is hybridized according to the kind A and probe of the fidelity of being traced to the source, gene extraction chip design is carried out and prepares, then
Chip is extracted using gene, capture extraction and enrichment are carried out to target gene and SNP segments.
Specifically, kind A contains Q genes, and gene order is SEQ ID NO in sequence table:It (is only shown here shown in 1
One DNA is single-stranded).
SEQ ID NO:1：ACAGGGGTGTTATGATGGAGTTTTAGC.
Using Q gene orders, specific capture oligonucleotide probe (such as SEQ ID NO are designed:Shown in 2)：
SEQ ID NO:2：TGTCCCCACAATACTACCTCAAAATCGA.
And for the individual A1 of kind A, the SNP information of A2 ..., A6 can design following sequence of 14- oligonucleotides and visit
Pin molecule：
SEQ ID NO:3：ACACAAGGTGTCCC；
SEQ ID NO:4：ATACGGTATACATA.
The following sequence table 11 of mentality of designing (SEQ ID NO:And (the SEQ ID NO of sequence table 12 5):6).
Single-molecule sequencing：Using nanometer pore single-molecule sequencing approach, target gene and SNP segments to capture are surveyed
Sequence obtains the hereditary information of detection sample.
Interpretation of result：Verification is compared with the hereditary information that gene label is marked in the hereditary information obtained, from
And realize fidelity of tracing to the source.
Analytical standard：All individuals belonged in the group of kind A all have Q genes；And belong to the individual B1 of kind B then
Q genes are not contained.If test sample contains Q genes, it can hybridize with designed probe molecule base complementrity and combine, so as to
It is captured to be enriched on chip；It is eluted to again afterwards in nanometer pore single-molecule sequencing and obtains sequence information, show that sample individual belongs to
In kind A.If sample to be tested does not contain Q genes, then can not be incorporated in designed probe molecule on genetic chip, so as to
It is then washed away, will not be entered in the nanometer pore single-molecule sequencing of next step, therefore obtained result is exactly no alkali
Motif column signal generates, and shows that sample individual is not belonging to kind A.
And for the individual A1 of kind A, A2 ..., A6, sample DNA can extract the SNP probe molecules on chip with gene
Base complementrity hybridization combines, and is enriched in so as to captured on extraction chip；It is eluted in nanometer pore single-molecule sequencing and obtains again afterwards
Sequence information is obtained, specific sample individual can be differentiated by the SNP information in sequencing sequence.
Analysis result：In the individual A1 of kind A, Q genes (as shown in the underscore in sequence) between A2 ..., A6, according to
General character gene identities information data of the feature Q gene sequence informations as all the individual A1, A2 ..., A6 of kind A in figure.
In other base data outside underlined sequences, there are single nucleotide polymorphism (SNP), with italicized bases code table in sequence table
Show.These SNP data messages then can be as the genetic fingerprint profile information of the Different Individual of the same kind of specific marker, area
The Different Individual A1, A2 ..., A6 of graded kind A.Wherein, the gene order of kind A, A1 such as SEQ ID NO:Shown in 7, the base of A2
Because of sequence such as SEQ ID NO:Shown in 8, the gene order such as SEQ ID NO of A3:Shown in 9, the gene order such as SEQ ID of A4
NO:Shown in 10, the gene order such as SEQ ID NO of A5:Shown in 11, the gene order such as SEQ ID NO of A6:Shown in 12.
And for kind B, the gene order such as SEQ ID NO of B1:Shown in 13, there is no Q genes, therefore A product are not belonging to
Kind.
B1:GGAGAAGAACACGTTGTGCATCAACCATTCAGGAAGGAGAATTAAAATCCTCTGGT G。
Embodiment two
The present embodiment provides a kind of real-time hereditary information acquisition methods, cooperation are simple and efficient using set forth in the present invention
Gene-tagging technology realizes the fidelity of tracing to the source of Yangcheng Lake steamed crab.
Due to the ChangJiang River subgroup of Yangcheng Lake steamed crab category Eriocheir sinensis, identify whether a crab individual is sun
Clear lake steamed crab is it needs to be determined that whether it belongs to Eriocheir sinensis.And because of " the Opp17 bases of DNA sequence dna specific to Eriocheir sinensis
Cause " is not present in other crab species.
Tissue treatment：The sample tissue of crab individual to be measured is taken, quickly grinds homogenate.
Nucleic acid extraction：Nucleic acid extraction is carried out using paramagnetic particle method, is obtained using the specific binding extraction of magnetic bead and nucleic acid high
The nucleic acid of purity, high concentration.
Target dna captures：Denaturation treatment is carried out to the nucleic acid that extraction obtains, then using specificity, for being traced to the source
The kind of fidelity using Opp17 gene sequence datas as the peculiar standard gene data of Eriocheir sinensis, designs Opp17 genes
Capture probe and prepare gene extraction chip, then chip is extracted using gene, capture extraction and enrichment is carried out to target gene.
Single-molecule sequencing：Results of hybridization is eluted, using nanometer pore single-molecule sequencing approach, to the target gene of capture into
Row sequencing obtains the hereditary information of detection sample.
Interpretation of result：Verification is compared with the hereditary information that gene label is marked in the hereditary information obtained, from
And realize fidelity of tracing to the source.Sequencing data is obtained then to show to contain Opp17 genes in the crab individual of detection, therefore, it is determined that detected
Crab individual belongs to Eriocheir sinensis, is Yangcheng Lake steamed crab；The individual something lost that sequencing data can be identified with gene label simultaneously
Communication breath, which do, further compares analysis.
Need the term explained as follows.Gene：It is the DNA fragmentation for carrying all hereditary information of bion, is control life
Object individual may be different from the basic genetic unit of the characters such as form, physiology, biochemistry and the behavior of other biological individual.
Genetic chip (also known as DNA chip, biochip)：Refer to anchor at the high-density DNA microarray on carrier.Specifically
Ground say be exactly using the target gene of known array or oligonucleotide fragment as nucleic acid probe in an orderly manner, high-density array glass,
On the carriers such as silicon, it is known as genetic chip.Genetic chip is now mainly used for genetic test purpose, by the sample DNA molecule of mark with
Nucleic acid probe on chip is hybridized and obtains sample molecule by detecting the hybridization signal intensities of each probe molecule
Quantity and sequence information.Used by the gene extraction chip illustrated in the present invention sample-probe hybridization matching principle with
The principle for the gene detecting chip routinely applied is identical but is used for the capture of gene enrichment purpose.
Single nucleotide polymorphism (SNP)：It refers to by single nucleotide acid-A, T, DNA sequence dna caused by the change of C or G
Change, cause the diversity of DNA sequence between species including humans.
It should be noted that unless otherwise indicated, technical term or scientific terminology used in this application should be this hair
The ordinary meaning that bright one of ordinary skill in the art are understood.Unless specifically stated otherwise, otherwise illustrate in these embodiments
Component and opposite step, numerical expression and the numerical value of step are not limit the scope of the invention.It is illustrated and described herein
In all examples, unless otherwise prescribed, any occurrence should be construed as merely illustrative, not as limitation, because
This, other examples of exemplary embodiment can have different values.
In the description of the present invention, it is to be understood that term " first ", " second " are only used for description purpose, and cannot
It is interpreted as indicating or implies relative importance or imply the quantity of the technical characteristic indicated by indicating.Define as a result, " the
One ", one or more this feature can be expressed or be implicitly included to the feature of " second ".In the description of the present invention,
" multiple " are meant that two or more, unless otherwise specifically defined.
Finally it should be noted that：The above embodiments are only used to illustrate the technical solution of the present invention., rather than its limitations；To the greatest extent
Pipe is described in detail the present invention with reference to foregoing embodiments, it will be understood by those of ordinary skill in the art that：Its according to
Can so modify to the technical solution recorded in foregoing embodiments either to which part or all technical characteristic into
Row equivalent substitution；And these modifications or replacement, the essence of appropriate technical solution is not made to depart from various embodiments of the present invention technology
The scope of scheme should all cover among protection scope of the present invention.
SEQUENCE LISTING
<110>Luan Tu, Chi Hai
<120>It is simple and efficient real-time hereditary information acquisition methods and its application
<130> 3
<160> 13
<170> PatentIn version 3.3
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acaggggtgt tatgatggag ttttagct 28
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acacacaagg tgtccccaca atactacctc aaaatcgatg atacggtata cataaaa 57
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tgtgtgttcc acaggggtgt tatgatggag ttttagctac tatgccatat gtatttt 57
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tctgtgttcc acaggggtgt tatgatggag ttttagctat tatgccatat gtatatt 57
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tctgtgttcc acaggggtgt tatgatggag ttttagctat tatgccatat gtatttt 57
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ggagaagaac acgttgtgca tcaaccattc aggaaggaga attaaaatcc tctggtg 57
Claims (10)
1. one kind is simple and efficient real-time hereditary information acquisition methods, it is characterised in that：
The method includes tissue treatment, nucleic acid extraction, target gene and/or the capture of SNP segments, single-molecule sequencing and knot successively
The step of fruit is analyzed.
2. according to claim 1 be simple and efficient real-time hereditary information acquisition methods, which is characterized in that at the tissue
Reason specifically includes：
The appropriate amount of sample of the tissue of agriculture and animal husbandry plant or animal is taken, grinds homogenate.
3. according to claim 1 be simple and efficient real-time hereditary information acquisition methods, which is characterized in that the nucleic acid carries
It takes and specifically includes：
Nucleic acid extraction is carried out using paramagnetic particle method, nucleic acid is obtained using the specific binding extraction of magnetic bead and nucleic acid.
4. according to claim 1 be simple and efficient real-time hereditary information acquisition methods, which is characterized in that the target base
Cause and/or the capture of SNP segments specifically include：
Denaturation treatment is carried out to the nucleic acid that extraction obtains, then using specificity, for the variety development base for the fidelity of being traced to the source
Because of extraction chip, then capture extraction and enrichment are carried out to target gene and/or SNP segments.
5. according to claim 4 be simple and efficient real-time hereditary information acquisition methods, it is characterised in that：
The gene extraction chip is designed and makes according to the matched principle of the kind and probe of the fidelity of being traced to the source hybridization
It is standby to form.
6. according to claim 1 be simple and efficient real-time hereditary information acquisition methods, which is characterized in that the unimolecule
Sequencing specifically includes：
Using nanometer pore single-molecule sequencing approach, target gene and/or SNP segments to capture are sequenced, and obtain detection sample
The hereditary information of product.
7. according to claim 1 be simple and efficient real-time hereditary information acquisition methods, which is characterized in that the result point
Analysis specifically includes：
Verification is compared with the hereditary information that gene label is marked in the hereditary information obtained, is protected so as to fulfill tracing to the source
Very.
8. claim 1-7 any one of them is simple and efficient real-time hereditary information acquisition methods to containing hereditary information
Product carries out the application in retrospect source.
9. application according to claim 8, it is characterised in that：
The product containing hereditary information is selected from agro-ecology, using agro-ecology as the food of raw material and using agro-ecology as original
One or more in the drug of material.
10. application according to claim 9, it is characterised in that：
One or more of the agro-ecology in crops, livestock and poultry and aquatic biological.
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