CN108040869B - Two-line sterile line breeding method suitable for multi-gene polymerization for mechanized mixed sowing seed production - Google Patents
Two-line sterile line breeding method suitable for multi-gene polymerization for mechanized mixed sowing seed production Download PDFInfo
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- CN108040869B CN108040869B CN201711306663.6A CN201711306663A CN108040869B CN 108040869 B CN108040869 B CN 108040869B CN 201711306663 A CN201711306663 A CN 201711306663A CN 108040869 B CN108040869 B CN 108040869B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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Abstract
The invention discloses a breeding method of a two-line sterile line with polygene polymerization suitable for mechanized mixed sowing seed production, which comprises the steps of hybridizing rice parents with recessive brown or hemp husk or black brown specific markers, endogenous genes containing herbicide resistance and dominant semi-dwarf genes, carrying out selfing on gibberellin-insensitive rice excellent two-line sterile lines, testing and matching with a restorer line, and selecting the two-line sterile line with high matching force and the genes and expression characteristics. The invention realizes polygene polymerization of two-line sterile lines, and sprays corresponding herbicide after pollination in the process of mixed sowing and seed production of the two-line sterile lines, thereby killing male parents and ensuring the production of qualified hybrid seeds; meanwhile, because the two-line sterile line is insensitive to gibberellin, the defects of over-high plant height and difficult pollination caused by gibberellin spraying in the seed production process are overcome, and the seed production yield is improved.
Description
Technical Field
The invention relates to the technical field of agriculture, in particular to a two-line sterile line breeding method suitable for multi-gene polymerization for mechanized mixed seeding seed production.
Background
With the development of the photoelectric color selection technology, the male parent and the female parent can be harvested in a mixed manner and sorted after mixed harvesting by breeding a specific female parent (sterile line). At present, color sorting mechanical equipment realizes sorting through husk color difference, high-precision sorting needs to be realized by using male and female parent seeds with large color difference, and meanwhile, under the condition of certain husk color difference, the proportion of the male parent seeds mixed in hybrid seeds is less, the higher the color sorting precision is, and the better the purity is. Patent CN 1454463A discloses a breeding method of hybrid rice parents and varieties with recessive markers, which provides basic materials and technical support for breeding specific sterile lines.
At present, gibberellin is sprayed in hybrid seeding and seed production of hybrid rice, so that the plant height of a female parent is too high or even higher than that of a male parent, and pollination is difficult.
The color difference of the male and female parent chaffs of most hybrid rice varieties is not obvious, and the quality of hybrid seeds can not be ensured to be qualified through photoelectric color selection, so that the application and popularization of the mechanized seed production technology of the hybrid rice are restricted.
Disclosure of Invention
The invention aims to provide a two-line sterile line breeding method suitable for multi-gene polymerization used for mechanized mixed sowing seed production.
The invention is realized by the following technical scheme:
a two-line sterile line breeding method suitable for multi-gene polymerization used for mechanized mixed sowing seed production comprises the following steps:
(1) hybridizing the hybrid rice parent with recessive specific marker with rice containing herbicide-resistant endogenous gene to obtain hybrid F1；
(2) From F, using a combination of conventional breeding and molecular Marker Assisted Selection (MAS)2Selecting a single plant which has a recessive specific marker and contains an herbicide-resistant endogenous gene in indoor molecular detection for selfing, and selecting a single plant which has the recessive specific marker, contains the herbicide-resistant homozygous gene and has stable agronomic characters after continuous selfing;
(3) hybridizing the stable single plant in the step (2) with a good two-line sterile line which carries dominant semi-dwarf gene and is insensitive to gibberellin, continuously selfing, and performing F2Selecting stable single plants with recessive specific markers, carrying herbicide-resistant endogenous genes and dominant semi-dwarf genes from generations, spraying gibberellin during heading, and screening stable and excellent two-line sterile lines which polymerize the genes and are insensitive to the gibberellin and have the recessive specific markers;
(4) and (4) testing and matching by using a restorer line, sampling and testing seeds when the restorer line is mature, and selecting the two-line sterile line which has higher combining ability and the characteristics in the step (3).
Wherein the hybrid rice glume with the recessive specific marker is brown, hemp hull or black brown.
The two-line sterile line with excellent stability bred by the invention introduces herbicide-resistant endogenous genes and dominant semi-dwarf genes into the two-line sterile line with recessive specific markers through molecular marker-assisted selection, and the sterile line is insensitive to gibberellin.
The two-line sterile line bred by the invention has the characteristics of recessive specific markers, carrying herbicide-resistant endogenous genes, dominant semi-dwarf genes, gibberellin insensitivity and the like, and the characteristics are derived from rice mutants, so that the transgenic safety problem does not exist.
The invention has the advantages that:
1) in the breeding process, a molecular marker-assisted selective breeding technology is adopted, so that the breeding efficiency is improved.
2) Grain safety: the selected herbicide-resistant endogenous gene is from a rice mutant, and compared with herbicide-resistant transgenic rice, the herbicide-resistant endogenous gene has no safety problem of the transgenic rice.
3) Quality assurance: due to the introduction of the herbicide-resistant gene, after pollination is completed, herbicide is sprayed to kill male parents effectively, qualified hybrid seeds can be obtained easily after screening by the color sorter, the maintenance frequency of the color sorter is reduced, the color sorting cost is reduced remarkably, and the purity of the seeds is guaranteed.
4) High efficiency: the invention introduces dominant semi-dwarf gene and has excellent gibberellin insensitive two-line sterile line, and gibberellin is sprayed in the mixed sowing seed production process of the two-line sterile line, so that the male parent is high, the female parent is low, pollination is easier, the seed production yield is increased, and the seed production efficiency is improved.
5) Mechanization in the whole process: the invention provides a method for breeding a two-line sterile line by multiple gene polymerization suitable for mechanized mixed sowing seed production, and provides technical guarantee for realizing whole-process mechanization of hybrid rice seed production.
Drawings
FIG. 1 is a schematic diagram of a two-line sterile line breeding method suitable for multi-gene polymerization for mechanized mixed sowing seed production;
FIG. 2 is a graph comparing brown-labeled hybrid rice seeds with normal-colored hybrid rice seeds.
Detailed Description
The present invention is described in further detail below with reference to fig. 1.
Example 1
In the following examples, the gene has brown marker 'Xinan S' and carries dominant semi-dwarfSD-d(t)And the 'all 151S' with excellent gibberellin insensitive is a self-breeding excellent two-line sterile line of Anhuoquan Yinjiao Seiko GmbH company; herbicide resistant materials the conventional material 'jieya rice 001' provided by xingwang biotechnology limited; the herbicide is mainly an imidazolinone herbicide, and the herbicide used in the embodiment is imazapic produced by basf european corporation, commonly known as "Bailingong".
The invention provides a method for breeding a sterile line by polygene polymerization suitable for mechanized mixed sowing seed production, which comprises the following steps:
(1) hybrid rice parent 'Xinan S' based on recessive brown marker and herbicide-resistant geneALSThe rice 'Jie tian rice 001' is hybridized to obtain a hybrid seed F1；
(2) From F, using a combination of conventional breeding and molecular Marker Assisted Selection (MAS)2Selection of genes with brown markers and herbicide resistance in indoor molecular detectionALSSelfing the single plants, and selecting the single plants which have brown marks, contain herbicide-resistant homozygous genes and have stable agronomic characters;
(3) the stable single plant and the dominant semi-dwarf gene in the step (2)SD-d(t)And crossing gibberellin insensitive excellent two-line sterile line 'full 151S', selfing continuously, and obtaining the sterile line from F2Selection of genes carrying herbicide resistance genes with brown markers from beginning of generationALSContaining dominant semi-dwarf geneSD-d(t)Stabilizing the individual plant, and extractingSpraying gibberellin during ear heading, and screening a stable and excellent sterile line which is insensitive to gibberellin and has the genes;
(4) testing and matching by using the restorer line as a male parent, screening the male parent and the female parent which have inconsistent reactions to gibberellin, namely male parent sensitivity and female parent insensitivity, sampling and testing seeds at maturity, and inspecting the combining ability condition of the sterile line; after pollination, spraying herbicide 'Bai Riong Tong' to ensure that the male parent dies and the female parent does not die, namely the male parent does not resist the herbicide and the female parent resists the herbicide;
(5) selecting a two-line sterile line which has high combining ability and meets the characteristics of the female parent in the step (4).
Claims (3)
1. A two-line sterile line breeding method suitable for multi-gene polymerization used for mechanized mixed sowing seed production is characterized by comprising the following steps:
(1) hybridizing the hybrid rice parent with recessive specific marker with rice containing herbicide-resistant endogenous gene to obtain hybrid F1；
(2) From F, a method combining conventional breeding and molecular marker-assisted selection is adopted2Selecting a single plant which has a recessive specific marker and contains an herbicide-resistant endogenous gene in indoor molecular detection for selfing, and selecting a single plant which has the recessive specific marker, contains the herbicide-resistant homozygous gene and has stable agronomic characters after continuous selfing;
(3) hybridizing the stable single plant in the step (2) with a good two-line sterile line which carries dominant semi-dwarf gene and is insensitive to gibberellin, continuously selfing, and performing F2Selecting stable single plants with recessive specific markers, carrying herbicide-resistant endogenous genes and dominant semi-dwarf genes from generations, spraying gibberellin during heading, and screening stable and excellent two-line sterile lines which polymerize the genes and are insensitive to the gibberellin and have the recessive specific markers;
(4) and (4) testing and matching by using a restorer line, sampling and testing seeds when the restorer line is mature, and selecting the two-line sterile line which has higher combining ability and the characteristics in the step (3).
2. The method for breeding the two-line sterile line of the polygene polymerization suitable for the mechanized mixed sowing seed production according to claim 1, wherein the bred two-line sterile line has polymerized herbicide-resistant endogenous gene and dominant semi-dwarf gene and has recessive specific marker.
3. The method for breeding the two-line sterile line with multi-gene polymerization suitable for mechanized mixed sowing seed production according to claim 1, wherein the bred two-line sterile line has herbicide-resistant property and is characterized by gibberellin bluntness, and glumes of the two-line sterile line have brown or black brown or hemp husk specific property.
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Inventor after: Huang Yanling Inventor after: Zhang Conghe Inventor after: Shen Guangle Inventor after: Fang Yu Inventor after: Wang Hui Inventor after: Chen Jinjie Inventor after: Yan Zhi Inventor after: Wang Lin Inventor after: Zhou Guixiang Inventor before: Huang Yanling Inventor before: Zhang Conghe Inventor before: Shen Guangqin Inventor before: Fang Yu Inventor before: Wang Hui Inventor before: Chen Jinjie Inventor before: Yan Zhi Inventor before: Wang Lin Inventor before: Zhou Guixiang |
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