CN108040538A - A kind of germination of Alfalfa seed and radicle growth measuring device - Google Patents
A kind of germination of Alfalfa seed and radicle growth measuring device Download PDFInfo
- Publication number
- CN108040538A CN108040538A CN201810026277.XA CN201810026277A CN108040538A CN 108040538 A CN108040538 A CN 108040538A CN 201810026277 A CN201810026277 A CN 201810026277A CN 108040538 A CN108040538 A CN 108040538A
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- seed
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- bowl
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
- A01C1/025—Testing seeds for determining their viability or germination capacity
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01B—MEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
- G01B5/00—Measuring arrangements characterised by the use of mechanical techniques
- G01B5/02—Measuring arrangements characterised by the use of mechanical techniques for measuring length, width or thickness
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- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a kind of germination of Alfalfa seed and radicle growth measuring devices, device body both sides, which are symmetrically installed, is useful for the body saddle that carrying seed sprouts bed, seed is sprouted is provided with several holes on the bottom surface of bed, and water storage spongy layer is equipped with by upper bottom plate in its bottom surface, seed sprouts the bottom of the bottom surface of bed and hole corresponding position thereon sets 4 capillary attraction feet, and capillary attraction foot gos deep into the inner cavity of case body；Seed is sprouted is provided with several placing of seed bowls on bed, several placing of seed bowls are the identical cone type bowl of multiple shapes, with the combination of rectangular close-packed arrays in one plane, embedded to be arranged in described hole, each cone type bowl is up and down opening, and funnel-form is presented；The lower end of each placing of seed bowl is both provided with the radicle measuring appliance body of test process seed.The present invention being capable of accurate evaluation germination quality.
Description
Technical field
The present invention relates to vegetable seeds physiological test technical fields, and in particular to a kind of germination of Alfalfa seed and radicle growth
Measuring device.
Background technology
Percentage of seedgermination refers to account for the percentage for inspection seed number in defined condition and the normal seedling number grown up in the time
Rate.《Crop seeds inspection procedure》Middle regulation, measuring percentage of seedgermination will be measured using germination test in laboratory conditions
Germination percentage.The equipment of germination test includes germination appliance and germinating bed.Germinator has germinating box, growing floor and germination vessel.
Germination vessel have germination box (ware) and germination disk.Germinating bed has paper bed and sand bed.When carrying out germination test, it will be arranged on germinating bed
Then germinating bed is placed on the bottom of germination box (ware) or the disk that germinates by tested seed, germination box is placed in temperature-controlled, illumination
Germinating box or growing floor in, cultivate in set point of temperature, illumination and in the time, investigation germination box (ware) interior germination seedling
Rate calculates germination percentage.
During germination test, the supply of moisture is very crucial to germination, and moisture is very little, seed can not sprout or
Delay is sprouted, and causes germination percentage relatively low；Excess moisture can then cause oxygen supply deficiency, cause rotten kind and pathogen breeding, together
Sample reduces percentage of seedgermination, and germination percentage result is caused not to be inconsistent with actual germination.And existing germination box (ware) does not have self-contr ol
Germinating bed (paper bed, sand bed and soil beds), the bottom of germination box (ware) is placed in during use by the function of moisture, and seed is arranged in hair
On bud bed.Moisture in germinating bed need to be added according to the experience of experimenter, especially with the paper bed chitting piece of poor water retention property,
To ensure the normal germination of seed, it is necessary to keep the skin wet daily in germination process.Since paper bed adsorptivity is poor, seed program request is given
Many difficulties are brought, and pelleted seed easily rolls on paper bed, and the kind bed being pre-placed is easily moved, and causes to count tired
It is difficult.Also, it slightly slips up in the process, the accuracy of percentage of seedgermination measure will be influenced.It is examined currently used for germination
The germinating bed species tested is more, but there are structure is more complicated, making is time-consuming, occupied space is larger, a sample survey
It is less or cannot be reused, use cost is higher etc..Therefore, in accordance with country《Crop seeds inspection procedure》
Principle under, transformation germination vessel reach automatic control germination moisture, ensure that moisture supply maintains the state suitable for germination
Under, the accuracy measured for percentage of seedgermination is just particularly important, this has very heavy for accurate evaluation seed quality
The practical significance wanted.
The germination index and vitality index of vegetable seeds are two important parameters for reflecting seed quality.And germination index and
Vitality index has highly important relation with the seedling length in the regular period.Therefore, vegetable seeds germination index and
Chitting piece must be taken out in the experiment of vitality index index determining from culture dish by grain, then measured and germinateed with vernier caliper
The radicle length of seed afterwards, it is not only time-consuming to take up one's energy, and make seed germination interrupt or even sometimes due to measurement etc. it is improper
Operation is likely to result in the tissue damage of chitting piece, is unfavorable for the development of follow-up test.
Clover is a kind of excellent leguminous forage, has important value in Animal husbandry production, because its seed is small, firmly
The features such as real rate is high, in practice how its germination of Accurate Determining and Seedling Growth Characteristics, there is an urgent need for a kind of seeds to sprout device, uses
It is of great practical significance in accurate evaluation seed quality.
The content of the invention
To solve the above problems, the present invention provides a kind of germination of Alfalfa seed and radicle growth measuring devices.
To achieve the above object, the technical solution taken of the present invention is：
A kind of germination of Alfalfa seed and radicle growth measuring device, including the matching case cover of device body
Bed is sprouted with seed, described device body both sides, which are symmetrically installed, is useful for the body saddle that carrying seed sprouts bed, the seed
It sprouts and is provided with several holes on the bottom surface of bed, and water storage spongy layer is equipped with by upper bottom plate in its bottom surface, described kind
Son sprouts the bottom of the bottom surface of bed and hole corresponding position thereon sets 4 capillary attraction feet, and the capillary attraction foot is deep
In the inner cavity for entering case body；To prevent nursery root growth from disk knot phenomenon and the growth convenient for early stage seedling, institute occur
It states and several placing of seed bowls is provided on seed sprouting bed, several placing of seed bowls are the identical cone type alms bowl of multiple shapes
Body, with the combination of rectangular close-packed arrays in one plane, embedded to be arranged in described hole, each cone type bowl is equal up and down
For opening, and funnel-form is presented；The lower end of each placing of seed bowl is both provided with the radicle measuring appliance sheet of test process seed
Body, the radicle measuring appliance body is a measuring appliance body in cylindrical shape being made by light transmissive material, described
Measuring appliance body it is open-ended, tubulose root elongated channel is equipped in the phase, and is set on the inner surface of the tubulose root elongated channel
It is equipped with length metering scale.
Preferably, the cube structure for the one end open that the case cover is formed for roof and covered body wall,
Roof is provided with venthole, for keeping the good aeration of mini measurement mechanism inner cavity mesostroma.
Preferably, the cube structure for the one end open that the case body bottom wall and side wall are formed, upper end one
Side is equipped with inlet, and lower end opposite side is equipped with leakage fluid dram, and the leakage fluid dram is provided with switching control pilot.
Preferably, the inner cavity of the placing of seed bowl is hemispherical or funnel-form.
Preferably, described hole is choana, and placing of seed bowl, which embeds, to be disposed therein.
Preferably, the placing of seed bowl funnel-form lower end sphere diameter is less than or equal to the grain size of test process seed
2/3。
Preferably, the tubulose root elongated channel inner cavity caliber upper end neck location is less than or equal to placing of seed bowl
Funnel-form lower end sphere diameter.
Preferably, the tubulose root elongated channel inner cavity caliber gives birth to radicle after being greater than or equal to germination to be measured
The 4/3 of root footpath.
The invention has the advantages that：
1st, the present invention takes out from culture dish the seed by grain germination without using vernier caliper that measure radicle long
Degree need to only read the radicle length reading for being stretched in each grain seed at the top of root elongated channel under the length metering scale of mark
Number.The index determinings heavy workload such as germination index and vitality index, often survey 1 sample under germination index just need measure 25 ×
The radicle length of 5 seeds, and the prior art needs 1 people measurement, 1 personal record, then 1 sample get off germination index and work
The index determinings such as power index just at least need 120min, and 1 personal hand-held record is only needed after mini measurement mechanism using the present invention
This, record when reading radicle length, can be easily accomplished within 30min, and then realize it is time saving and energy saving, and favorably
It is identified in seedling, improves test accuracy.
2nd, the present invention experiment during do not have to contact seed each tissue site, also would not as the prior art,
Seed to be measured from culture dish is taken out, is effectively prevented during seed base-root length is measured, due to measurement etc. no
The proper tissue damage for causing chitting piece, is unfavorable for the development of follow-up test.And hair will be made using this mini measurement mechanism
Bud experiment be provided with can continuity, and be conducive to carry out follow-up every research work.
3rd, the present invention is expansible is widely used in the fields such as houseplant seed is sprouted and seedling is cultivated.It is simple in structure, can
Add water workload to save, efficiently using liquid manure resource, ensure good microenvironment of seedling root growth etc..
Description of the drawings
Fig. 1 is a kind of " germination of Alfalfa seed and radicle length measuring device " of embodiment of the present invention main structure diagram.
Fig. 2 is a kind of " germination of Alfalfa seed and radicle length measuring device " of embodiment of the present invention overlooking the structure diagram.
Fig. 3 is a kind of " germination of Alfalfa seed and radicle length measuring device " of embodiment of the present invention schematic cross-sectional view.
In figure：1 case cover；2 seeds sprout bed；3 case bodies；31 body saddles；Bottom plate on 4；5
Water storage spongy layer；6 placing of seed bowls；7 radicle measuring appliance bodies；8 capillary attraction feet；9 inlets；10 leakage fluid drams；11 open
Close control valve；12 ventholes；71 length metering scales；72 elongated channels；81st, 82,83 and 84 capillary attraction foot.
Specific embodiment
In order to which objects and advantages of the present invention are more clearly understood, the present invention is carried out with reference to embodiments further
It is described in detail.It should be appreciated that specific embodiment described herein is not intended to limit the present invention only to explain the present invention.
As shown in Figure 1-Figure 3, an embodiment of the present invention provides a kind of germination of Alfalfa seed and radicle growth measuring device,
It is characterized in that, sprouts bed 2,3 both sides of described device body including the matching case cover 1 of device body 3 and seed
It is symmetrically installed and is useful for the body saddle 31 that carrying seed sprouts bed 2, the seed is sprouted is provided with several holes on the bottom surface of bed 2
Hole, and water storage spongy layer 5 is equipped with by upper bottom plate 4 in its bottom surface, the seed sprout the bottom surface of bed 2 bottom and its
On hole corresponding position 4 capillary attraction feet 8 are set, the capillary attraction foot 8 gos deep into the inner cavity of case body 3
In；To prevent nursery root growth from disk knot phenomenon and the growth convenient for early stage seedling occur, the seed is sprouted to be provided on bed 2
Several placing of seed bowls 6, several placing of seed bowls 6 are the identical cone type bowl of multiple shapes, with rectangular close row
Row combination is in one plane, embedded to be arranged in described hole, and each cone type bowl is up and down opening, and funnel is presented
Shape；The lower end of each placing of seed bowl 6 is both provided with the radicle measuring appliance body 7 of test process seed, and the radicle is surveyed
Measuring device body 7 be a measuring appliance body in cylindrical shape being made by light transmissive material, the end of the measuring appliance body
Portion's opening, it is interior to be equipped with tubulose root elongated channel 72, and it is provided with length gauge on the inner surface of the tubulose root elongated channel 72
Measure scale 71.
The cube structure for the one end open that the case cover 1 is formed for roof and covered body wall, roof are set
Venthole 12 is equipped with, for keeping the good aeration of mini measurement mechanism inner cavity mesostroma.3 bottom wall of case body
And the cube structure of the one end open of side wall composition, upper end one side are equipped with inlet 9, lower end opposite side is equipped with leakage fluid dram
10, the leakage fluid dram 10 is provided with switching control pilot 11.The inner cavity of the placing of seed bowl 6 is hemispherical or funnel-form.Institute
Hole is stated as choana, placing of seed bowl 6 is embedded to be located at wherein, so on the one hand may insure alfalfa seed energy
It is enough to make somebody a mere figurehead the normal germinating growth in the placing of seed bowl 6 during seed sprouts bed 2, the water storage spongy layer 5 described in another aspect
The moisture in case body 3 and treatment fluid are absorbed into seed by capillary attraction foot 8 to sprout in bed 2, and then continue,
It slowly enters in placing of seed bowl 6, realizes from mini measurement mechanism inner cavity mesostroma lower part moist automatic watering and supplement
Treatment fluid solves the tedious work of timing adding water fertilising, can also efficiently utilize moisture and treatment fluid, reduces moisture and processing
Liquid scatters and disappears, and excessively keeps the skin wet even if sprouting 2 top of bed from seed, since seed sprouts bed 2 and 3 phase of case body
It is logical, seed will not be caused to sprout 2 moisture of bed excess, and matrix aeration is influenced, reach automatic control germination moisture, ensure that moisture supplies
Always it should ensure that the good growth microenvironment in seedling root in the state of suitable for germination.The placing of seed alms bowl
6 funnel-form lower end sphere diameter of body is less than or equal to the 2/3 of the grain size of test process seed.72 inner cavity of tubulose root elongated channel
Caliber upper end neck location is less than or equal to 6 funnel-form lower end sphere diameter of placing of seed bowl.The tubulose root elongated channel 72
Inner cavity caliber gives birth to the 4/3 of radicle root footpath after being greater than or equal to germination to be measured.
Embodiment
With leguminous seeds alfalfa (Medicago sativa L.) germination index and vitality index in following embodiment
It is illustrated exemplified by measure.Alfalfa leguminous seeds germination index and vitality index are measured with the seedling length in the regular period
Micro device, the long 70mm of radicle measuring appliance body above have the placing of seed bowl that sphere diameter is 1.5-2mm, tubulose root elongated channel
Inner cavity caliber upper end neck location sphere diameter for 1.5-2mm, the inner cavity caliber of tubulose root elongated channel is 3-3.5mm.During experiment,
Each component of measuring device influenced first on a kind of germination of Alfalfa seed and radicle growth assembles, by distilled water or treatment fluid
It is input by inlet in case chamber body, case body is made to obtain the distilled water or treatment fluid of 30ml；Secondly
Case cover is opened, checks the aeration status of venthole, and using distilled water or treatment fluid to placing of seed bowl and storage
Water spongy layer is soaked, and selected full uniform alfalfa seed, and 20min, distillation are sterilized using liquor natrii hypochloritis
Water rinses 3 times；Again with 70% alcohol disinfecting 30s, distilled water flushing 3 times beaten easily with tweezers and places it in the placing of seed
In bowl；It is again placed in (23 ± 2) DEG C, light application time is germination and growth under 16D/8N.Each 25 seeds of processing set 5
A repetition；Finally germinate during every for 24 hours observe different disposal under the conditions of alfalfa seed sprouting situation, statistics seed sprout
Number is sent out, using radicle and seed is isometric, radicel length reaches seed half as the basis for estimation of germination.And pass through registration and show
Show and count its radicle length, calculate the growths such as germination percentage, germinating energy, average germinating time (MGT), germination index and vitality index
Index.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, without departing from the principle of the present invention, several improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (8)
1. a kind of germination of Alfalfa seed and radicle growth measuring device, which is characterized in that including the matching appearance of device body
Device device cover and seed sprout bed, and described device body both sides, which are symmetrically installed, is useful for the body support that carrying seed sprouts bed
Platform, the seed, which is sprouted, is provided with several holes on the bottom surface of bed, and is equipped with water storage sea by upper bottom plate in its bottom surface
Continuous layer, the seed sprouts the bottom of the bottom surface of bed and hole corresponding position thereon sets 4 capillary attraction feet, the capillary
Pipe attracts foot to go deep into the inner cavity of case body；The seed is sprouted is provided with several placing of seed bowls on bed, several
Placing of seed bowl is the identical cone type bowl of multiple shapes, with the combination of rectangular close-packed arrays in one plane, is embedded
It is arranged in described hole, each cone type bowl is up and down opening, and funnel-form is presented；Under each placing of seed bowl
End is both provided with the radicle measuring appliance body of test process seed, and the radicle measuring appliance body is made for one by light transmissive material
The measuring appliance body in cylindrical shape formed, the measuring appliance body it is open-ended, it is logical that the elongation of tubulose root is equipped in the phase
Road, and it is provided with length metering scale on the inner surface of the tubulose root elongated channel.
2. a kind of germination of Alfalfa seed as described in claim 1 and radicle growth measuring device, which is characterized in that the container
The cube structure for the one end open that device cover is formed for roof and covered body wall, roof are provided with venthole.
3. a kind of germination of Alfalfa seed as described in claim 1 and radicle growth measuring device, which is characterized in that the container
The cube structure for the one end open that device body bottom wall and side wall are formed, upper end one side are equipped with inlet, lower end opposite side
Equipped with a leakage fluid dram, the leakage fluid dram is provided with switching control pilot.
4. a kind of germination of Alfalfa seed as described in claim 1 and radicle growth measuring device, which is characterized in that the seed
The inner cavity for positioning bowl is hemispherical or funnel-form.
5. a kind of germination of Alfalfa seed as described in claim 1 and radicle growth measuring device, which is characterized in that described hole
For choana, placing of seed bowl, which embeds, to be disposed therein.
6. a kind of germination of Alfalfa seed as described in claim 1 and radicle growth measuring device, which is characterized in that the kind
Son positioning bowl funnel-form lower end sphere diameter is less than or equal to the 2/3 of the grain size of test process seed.
7. a kind of germination of Alfalfa seed as described in claim 1 and radicle growth measuring device, which is characterized in that the pipe
Shape root elongated channel inner cavity caliber upper end neck location is less than or equal to placing of seed bowl funnel-form lower end sphere diameter.
8. a kind of germination of Alfalfa seed as described in claim 1 and radicle growth measuring device, which is characterized in that the pipe
Shape root elongated channel inner cavity caliber gives birth to the 4/3 of radicle root footpath after being greater than or equal to germination to be measured.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810026277.XA CN108040538A (en) | 2018-01-07 | 2018-01-07 | A kind of germination of Alfalfa seed and radicle growth measuring device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810026277.XA CN108040538A (en) | 2018-01-07 | 2018-01-07 | A kind of germination of Alfalfa seed and radicle growth measuring device |
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| Publication Number | Publication Date |
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| CN108040538A true CN108040538A (en) | 2018-05-18 |
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ID=62126382
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| CN201810026277.XA Pending CN108040538A (en) | 2018-01-07 | 2018-01-07 | A kind of germination of Alfalfa seed and radicle growth measuring device |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109258441A (en) * | 2018-10-24 | 2019-01-25 | 杭州师范大学 | Plant forms observe hydroponic device and application method |
| CN109373853A (en) * | 2018-10-24 | 2019-02-22 | 杭州师范大学 | Water culture and root measurement device and application method |
| CN109430028A (en) * | 2018-10-24 | 2019-03-08 | 杭州师范大学 | Multi-functional submerged plant form observation incubator and application method |
| CN109792942A (en) * | 2018-10-24 | 2019-05-24 | 杭州师范大学 | Water plant culture and rhizome measuring platform and operating method |
| CN110352726A (en) * | 2019-08-23 | 2019-10-22 | 重庆市农业科学院 | Seed is vertically taken root device |
| CN111528667A (en) * | 2020-05-08 | 2020-08-14 | 郭春燕 | Cooling cup for summer |
| CN113390313A (en) * | 2021-06-10 | 2021-09-14 | 东风(武汉)实业有限公司 | Novel battery box module detection device |
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| CN109430028A (en) * | 2018-10-24 | 2019-03-08 | 杭州师范大学 | Multi-functional submerged plant form observation incubator and application method |
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| CN109792942B (en) * | 2018-10-24 | 2024-04-26 | 杭州师范大学 | Aquatic plant cultivation and rhizome observation table and operation method |
| CN109430028B (en) * | 2018-10-24 | 2024-05-14 | 杭州师范大学 | Multifunctional submerged plant morphological observation incubator and use method thereof |
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| CN111528667A (en) * | 2020-05-08 | 2020-08-14 | 郭春燕 | Cooling cup for summer |
| CN113390313A (en) * | 2021-06-10 | 2021-09-14 | 东风(武汉)实业有限公司 | Novel battery box module detection device |
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