CN108036988A - A kind of sperm quick dyeing liquid - Google Patents
A kind of sperm quick dyeing liquid Download PDFInfo
- Publication number
- CN108036988A CN108036988A CN201711347564.2A CN201711347564A CN108036988A CN 108036988 A CN108036988 A CN 108036988A CN 201711347564 A CN201711347564 A CN 201711347564A CN 108036988 A CN108036988 A CN 108036988A
- Authority
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- China
- Prior art keywords
- sperm
- quick
- dyeing
- dyeing liquid
- blue
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- Pending
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- 238000004043 dyeing Methods 0.000 title claims abstract description 28
- 239000007788 liquid Substances 0.000 title claims abstract description 17
- OARRHUQTFTUEOS-UHFFFAOYSA-N safranin Chemical compound [Cl-].C=12C=C(N)C(C)=CC2=NC2=CC(C)=C(N)C=C2[N+]=1C1=CC=CC=C1 OARRHUQTFTUEOS-UHFFFAOYSA-N 0.000 claims abstract description 15
- 125000000853 cresyl group Chemical group C1(=CC=C(C=C1)C)* 0.000 claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 7
- NZYCYASKVWSANA-UHFFFAOYSA-M new methylene blue Chemical compound [Cl-].CCNC1=C(C)C=C2N=C(C=C(C(NCC)=C3)C)C3=[S+]C2=C1 NZYCYASKVWSANA-UHFFFAOYSA-M 0.000 claims abstract 3
- 238000000034 method Methods 0.000 abstract description 22
- 238000003745 diagnosis Methods 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 4
- 238000003908 quality control method Methods 0.000 abstract description 4
- 239000003086 colorant Substances 0.000 abstract description 2
- 201000010099 disease Diseases 0.000 abstract description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 2
- 230000001568 sexual effect Effects 0.000 abstract description 2
- 238000010186 staining Methods 0.000 description 9
- 238000004040 coloring Methods 0.000 description 8
- SQHOAFZGYFNDQX-UHFFFAOYSA-N ethyl-[7-(ethylamino)-2,8-dimethylphenothiazin-3-ylidene]azanium;chloride Chemical compound [Cl-].S1C2=CC(=[NH+]CC)C(C)=CC2=NC2=C1C=C(NCC)C(C)=C2 SQHOAFZGYFNDQX-UHFFFAOYSA-N 0.000 description 8
- 210000000582 semen Anatomy 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 230000007812 deficiency Effects 0.000 description 5
- 230000000877 morphologic effect Effects 0.000 description 4
- 238000011121 vaginal smear Methods 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 3
- 238000005034 decoration Methods 0.000 description 3
- 231100000614 poison Toxicity 0.000 description 3
- 230000007096 poisonous effect Effects 0.000 description 3
- 239000007853 buffer solution Substances 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 2
- 231100000004 severe toxicity Toxicity 0.000 description 2
- 230000009897 systematic effect Effects 0.000 description 2
- WULISCVZERSMML-UHFFFAOYSA-O 3,7-dimethyl-10-phenylphenazin-10-ium-2,8-diamine Chemical compound C=12C=C(N)C(C)=CC2=NC2=CC(C)=C(N)C=C2[N+]=1C1=CC=CC=C1 WULISCVZERSMML-UHFFFAOYSA-O 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 241001062009 Indigofera Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000002360 explosive Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 208000017517 male reproductive system disease Diseases 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000011206 morphological examination Methods 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a kind of sperm quick dyeing liquid, include the component of following mass percent：0.01%~50% brilliant cresyl blue, 0.01%~50% safranin O, 0.01%~50% new methylene blue, and excess water.A kind of sperm quick dyeing liquid provided by the present invention, rapid dyeing is carried out to Ejaculated sperm cells using one-step method, the sperm in sperm and various cells can be made to catch different colors in five seconds, make sperm non-clear, so as to quick and precisely be identified, reliable diagnosis basis is provided for clinic, the Accurate Diagnosis and discriminating and accurate treatment to disease in the male sexual system play a significant role, and extremely important effect will be played in the standardization and quality control of sperm dyeing.
Description
Technical field
The present invention relates to a kind of sperm dye liquor product technical field, particularly a kind of sperm quick dyeing liquid.
Background technology
After spermatoblast staining technique refers to sperm in sperm by chemical staining, show various micro- inside sperm
Small structure, easy to observe its form, reflects the morphological feature under physiology and pathological state.Ejaculated sperm cells morphological examination is to facing
Bed male reproductive system disease differentiates there is value of crucial importance with diagnosis, and Ejaculated sperm cells staining technique is the weight of Semen routione project
Want part.
Sperm morphological inspection in Semen routione project, generally use is auspicious-and Ji's Albert'stain Albert method (grasps see national clinical examination
Make code third edition P123~124 page) pap staining (see national clinical examination operational procedure third edition P325~326 page) and Soviet Union
H ＆ E dyeing is (see national clinical examination operational procedure third edition P326~327 page) etc., and there is behaviour for these colouring methods
Make complicated, dyeing time length, many deficiencies such as colour developing is enough unstable and reagent is poisonous.
Specifically, the deficiency that auspicious-Ji's Albert'stain Albert method is primarily present is：To its unstable result of staining of sperm, easily by
The influence of the acid-base value of semen sample, slide and buffer solution, dyeing time is longer (15~30 minutes), to perforatorium structure and
Tail structure coloring is not ideal enough；And containing easily burning, poisonous methanol in auspicious-Ji Shi dye liquors, there are security risk.
The deficiency that Papanicolaou's vaginal smear technique is primarily present is：Coloring is preferable, but still is not sufficiently stable, and dyeing course
Very miscellaneous multiple, operation step is poly- more, poly- comprising more than ten of operation step；Dyeing time up to 1 it is small when more than, in reagent containing severe toxicity
Mercury metal, pollutes environment.
The deficiency that Hematoxylin-eosin decoration method is primarily present is：It is a kind of easy Papanicolaou's vaginal smear technique in fact, dyeing effect
Fruit is not so good as Pasteur, but dyeing course is simple compared with Pasteur's method, and operation step is poly- poly- comprising six steps；When dyeing time length nearly 1 is small,
Mercury metal containing severe toxicity in reagent, pollutes environment.
The content of the invention
For overcome the deficiencies in the prior art, the present invention provides a kind of easy, quick, safe and efficient staining of sperm
Liquid.
The technical solution adopted by the present invention to solve the technical problems is：
A kind of sperm quick dyeing liquid, including：Brilliant cresyl blue, safranin O and new methylene blue.
Wherein：
Brilliant cresyl blue, alias is for brilliant cresyl blue, cresyl blue, bright toluene sweet smell indigo plant etc., molecular formula：
C17H21CLN4O,
English name Brilliant cresyl blue, systematic naming method legitimate name：
3-Amino-9-diethylamino-10-methyldiphenazoxonium chloride。
Safranin O, alias is husky of common dye, safranine T, blue light safranine, rhodamine, safranine T, cotton are red, safranine T
Deng molecular formula：C20H19CLN4,
[H2NC6H2(CH3)N:C6H2(NH2)(CH3):N (CL) C6H5],
English name Safranine O, systematic naming method legitimate name：
3,7-Diamino-2,8-dimethyl-5-phenylphenazinium。
New methylene blue, molecular formula：C36H44Cl4N6S2Zn,
English name New methylene blue N.
As the further preferred of above-mentioned technical proposal, include the component of following mass percent：
0.01%~50% brilliant cresyl blue,
0.01%~50% safranin O,
0.01%~50% new methylene blue,
And excess water.
Compared with prior art, the beneficial effects of the invention are as follows：Rapid dyeing is carried out to Ejaculated sperm cells using one-step method,
The sperm in sperm and various cells can be made to catch different colors in five seconds, make sperm non-clear, so as to obtain
Quick and precisely identify, reliable diagnosis basis is provided for clinic, Accurate Diagnosis and discriminating and essence to disease in the male sexual system
Quasi- treatment plays a significant role, and extremely important effect will be played in the standardization and quality control of sperm dyeing.
Specifically, has the following advantages：
1st, it is easy to operate：Smear, Huang Shi is added dropwise, and quickly smart dye liquor, capping slide can micro- sem observation spermatoblasts immediately
Form；
2nd, dye ultrafast：Huang Shi is added dropwise quickly five seconds can microscopy after smart dye liquor；
3rd, coloring is good：Acrosome, head, neck and afterbody coloring after staining of sperm is very clear, is very easy to know
Not；
4th, normalizing operation easy to implement is dyed：Operation step is poly- few, very easy, be easily mastered, and influence factor is few, from
The acid-base value of semen sample, slide and buffer solution influences, and is adapted to formulating standard operational procedure；
5th, quality control：Acrosome, head, neck and afterbody coloring ten after staining of sperm are distinguished, and are very easy in identification,
Improve sperm morphological quality level；
6th, reagent safety：Any poisonous, harmful, inflammable, explosive material is free of in agent formulations, securely and reliably.
Brief description of the drawings
The present invention is further described with reference to the accompanying drawings and examples.
Fig. 1 is the smart dye liquor photo that the specific embodiment of the invention obtains；
Fig. 2 is the smart dye liquor photo that comparative examples of the present invention are obtained according to auspicious-Ji's Albert'stain Albert method；
Fig. 3 is the smart dye liquor photo that comparative examples of the present invention are obtained according to Papanicolaou's vaginal smear technique；
Fig. 4 is the smart dye liquor photo that comparative examples of the present invention are obtained according to Hematoxylin-eosin decoration method.
Embodiment
Below in conjunction with specific embodiments come be further described the present invention technology contents.
Specific embodiment 1
A kind of sperm quick dyeing liquid that the present embodiment is provided, includes the component of following mass percent：
10% brilliant cresyl blue,
50% safranin O,
15% new methylene blue,
And excess water.
Specific embodiment 2
A kind of sperm quick dyeing liquid that the present embodiment is provided, includes the component of following mass percent：
50% brilliant cresyl blue,
20% safranin O,
10% new methylene blue,
And excess water.
Specific embodiment 3
A kind of sperm quick dyeing liquid that the present embodiment is provided, includes the component of following mass percent：
20% brilliant cresyl blue,
10% safranin O,
50% new methylene blue,
And excess water.
Specific embodiment 4
A kind of sperm quick dyeing liquid that the present embodiment is provided, includes the component of following mass percent：
0.01% brilliant cresyl blue,
0.01% safranin O,
0.01% new methylene blue,
And excess water.
Semen sample smear is dried (or not drying), it is quick that a kind of sperm made from 2 drop specific embodiments 1~3 is added dropwise
Dye liquor, capping slide flatten, and the photo obtained under the microscope is as shown in Figure 1.
By semen sample smear, without drying, directly a kind of sperm made from the drop of dropwise addition 2 specific embodiments 1~3 quickly contaminates
Liquid, capping slide flatten, and the photo obtained under the microscope is as shown in Figure 1.
Semen sample smear is dried, a kind of sperm quick dyeing liquid made from 10 drop specific embodiments 4, capping slide is added dropwise
Flatten, the photo obtained under the microscope is as shown in Figure 1.
Comparative examples 1
Smart dye liquor is prepared according to auspicious-Ji's Albert'stain Albert method, the photo obtained under the microscope is as shown in Figure 2.
Comparative examples 2
Smart dye liquor is prepared according to Papanicolaou's vaginal smear technique, the photo obtained under the microscope is as shown in Figure 3.
Comparative examples 3
Smart dye liquor is prepared according to Hematoxylin-eosin decoration method, the photo obtained under the microscope is as shown in Figure 4.
According to above-described embodiment：
A kind of sperm quick dyeing liquid provided by the present invention, when operation, only need a step, that is, be added dropwise Huang Shi quickly smart dye liquor in
It can complete to dye in sperm body mark, in five seconds, distinguish the acrosome after staining of sperm, head, neck and afterbody coloring ten
It is clear visible；Have the advantages that easy to operate, dyeing is quick, reagent safety, coloring are good.
A kind of sperm quick dyeing liquid provided by the present invention, the clinical practice through 500 many cases, achieves extraordinary effect
Fruit；Especially suitable for normalizing operation, also solve the problems, such as the big of sperm morphological quality control.
Above the preferable of the present invention is implemented to be illustrated, certainly, the present invention can also use and above-mentioned implementation
The different form of mode, the equivalent conversion that those skilled in the art are made on the premise of without prejudice to spirit of the invention
Or corresponding change, it should all belong in protection scope of the present invention.
Claims (2)
- A kind of 1. sperm quick dyeing liquid, it is characterised in that：Including brilliant cresyl blue, safranin O and new methylene blue.
- 2. a kind of sperm quick dyeing liquid according to claim 1, it is characterised in that include the group of following mass percent Point：0.01%~50% brilliant cresyl blue,0.01%~50% safranin O,0.01%~50% new methylene blue,And excess water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711347564.2A CN108036988A (en) | 2017-12-15 | 2017-12-15 | A kind of sperm quick dyeing liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711347564.2A CN108036988A (en) | 2017-12-15 | 2017-12-15 | A kind of sperm quick dyeing liquid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108036988A true CN108036988A (en) | 2018-05-15 |
Family
ID=62102712
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711347564.2A Pending CN108036988A (en) | 2017-12-15 | 2017-12-15 | A kind of sperm quick dyeing liquid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108036988A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4185085A (en) * | 1976-11-09 | 1980-01-22 | Boehringer Mannheim Gmbh | Differential diagnostic sperm examination |
| CN1584545A (en) * | 2004-06-16 | 2005-02-23 | 深圳华康生物医学工程有限公司 | Sperm morphology rapid dyeing reagent and method for rapid dyeing sperm |
| CN105102960A (en) * | 2013-03-15 | 2015-11-25 | 艾瑞思国际股份有限公司 | Method and composition for staining and processing a urine sample |
| CN105440725A (en) * | 2008-01-04 | 2016-03-30 | 深圳迈瑞生物医疗电子股份有限公司 | Asymmetric cyanine fluorescent dye, composition and application in biological sample dyeing |
-
2017
- 2017-12-15 CN CN201711347564.2A patent/CN108036988A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4185085A (en) * | 1976-11-09 | 1980-01-22 | Boehringer Mannheim Gmbh | Differential diagnostic sperm examination |
| CN1584545A (en) * | 2004-06-16 | 2005-02-23 | 深圳华康生物医学工程有限公司 | Sperm morphology rapid dyeing reagent and method for rapid dyeing sperm |
| CN105440725A (en) * | 2008-01-04 | 2016-03-30 | 深圳迈瑞生物医疗电子股份有限公司 | Asymmetric cyanine fluorescent dye, composition and application in biological sample dyeing |
| CN105102960A (en) * | 2013-03-15 | 2015-11-25 | 艾瑞思国际股份有限公司 | Method and composition for staining and processing a urine sample |
Non-Patent Citations (1)
| Title |
|---|
| 项晓琼等: "一种染琼脂糖凝胶中DNA的新方法", 《军事医学科学院院刊》 * |
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| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180515 |
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| RJ01 | Rejection of invention patent application after publication |