CN108029451A - A kind of lucid ganoderma stock culture culture medium and preparation method thereof - Google Patents
A kind of lucid ganoderma stock culture culture medium and preparation method thereof Download PDFInfo
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- CN108029451A CN108029451A CN201711236476.5A CN201711236476A CN108029451A CN 108029451 A CN108029451 A CN 108029451A CN 201711236476 A CN201711236476 A CN 201711236476A CN 108029451 A CN108029451 A CN 108029451A
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- culture medium
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- sorghum rice
- lucid ganoderma
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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Abstract
The present invention relates to fungi culture medium preparation field, particularly discloses a kind of lucid ganoderma stock culture culture medium and preparation method thereof.The lucid ganoderma stock culture culture medium, it is characterized in that, it is made of following raw material：Sorghum rice cooking water 1000mL, 20 40g of glucose, 2 4g of peptone, 2 4g of potassium dihydrogen phosphate, 2 4g of magnesium sulfate, 15 25g of agar.The present invention is prepared simply, and the lucid ganoderma stock culture speed of growth that culture medium spreads cultivation is fast, and growing way is vigorous, and switching contamination rate is low, and the quality that the same terms lower later stage obtains ganoderma lucidum product is better than the ganoderma lucidum that common mother culture media spreads cultivation.
Description
（One）Technical field
The present invention relates to fungi culture medium preparation field, more particularly to a kind of lucid ganoderma stock culture culture medium and preparation method thereof.
（Two）Background technology
Ganoderma lucidum（Ganoderma lucidum）It is a kind of food, the fungi of medicine dual-purpose, contains ganoderma lucidum polysaccharide, triterpene, ganoderic acid, more
The several functions component such as peptide, amino acid, in China's medicinal history of existing more than 2000 years, has and consolidates righting, antitumor, beneficial
Many effects, the market demands such as liver, raising immunity of organisms are huge.The development of ornamental value ganoderma lucidum in recent years, further promotees
The development of industry is manually cultivated into ganoderma lucidum.
Under normal conditions, ganoderma lucidum it is extensive manually culture need to pass through Mother culture, Primary spawn, cultigen culture and
Go out several processes such as sesame culture.In the spreading cultivation of ganoderma lucidum, it is to obtain the guarantee of high yield to carry out switching using strong mycelium.Its
In, the mycelial speed of growth of lucid ganoderma stock culture and growth potential have a significant impact the production efficiency and later product quality of ganoderma lucidum.
And culture medium is to influence one of ganoderma lucidum mycelium growth conditions most important factor, therefore select a kind of efficient lucid ganoderma stock culture culture
Base, has larger realistic meaning.
（Three）The content of the invention
The spirit simple, production cost is low, culture efficiency is high is prepared in order to compensate for the shortcomings of the prior art, the present invention provides a kind of
Sesame mother culture media and preparation method thereof.
The present invention is achieved through the following technical solutions：
A kind of lucid ganoderma stock culture culture medium, it is characterized in that, it is made of following raw material：Sorghum rice cooking water 1000mL, glucose 20-
40g, peptone 2-4g, potassium dihydrogen phosphate 2-4g, magnesium sulfate 2-4g, agar 15-25g.
The optimum ratio of each raw material is：
Sorghum rice cooking water 1000mL, glucose 30g, peptone 3g, potassium dihydrogen phosphate 3g, magnesium sulfate 3g, agar 20g.
The preparation method of above-mentioned lucid ganoderma stock culture culture medium, includes the following steps：
（1）Sorghum rice adds water and quick lime to soak, and is transferred to after rinsing afterwards in steamer, adds water boiling, then filtered, flushing to obtain
Sorghum rice cooking water；
（2）Sorghum rice cooking water 1000mL is taken, adds glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate and agar, stirring, add
Heat, moisturizing is to 1000mL after agar dissolves, and adjusts pH value；
（3）Prepared culture medium is sub-packed in test tube, autoclave sterilizing is put into after brown paper wrapping, inclined-plane is put, after cooling
Remember mother culture media test tube slant.
Its more excellent technical solution is：
Step（1）In, sorghum rice 500g, which is added water to, does not cross surface 2cm, adds quick lime, 24h is soaked after mixing, afterwards with originally
Water cleans sorghum rice, is transferred in steamer, adds water to and does not cross surface, and slow boiling boiling 15-25min is full to grain of rice expansion, but not
Rupture, boiling terminate, and the strainer filtering of grain of rice size is less than with eyelet, then with warm water washing and filter, and merging filtrate, obtains height
Fine strain of millet rice cooking water 1000mL.
Step（1）In, after the sorghum rice filtering after boiling, rinsed and filtered, two with 50-60 DEG C of warm water 200-500mL
Secondary gained filtrate merges.Advanced age rice is full to expanding using slow boiling boiling during steamer, must not rupture sorghum rice.
Step（3）In, prepared culture medium is sub-packed in test tube while hot, and liquid amount is no more than test tube（20×200 mm）
The 1/3 of height, when packing, prevent that culture medium adheres to test tube mouth, silica gel plug sealing, brown paper wrapping after packing, in 121 DEG C
The lower sterilizing 20min or 30min that sterilizes at 105 DEG C, puts inclined-plane, to obtain the final product.
Wherein, the term in the present invention is explained as follows：
Parent species：Ganoderma lucidum incubation test tube strains, also known as first order seed；
Original seed：Ganoderma lucidum incubation spreads cultivation strain, is generally cultivated in vial, also known as secondary seed；
Culture medium：The nutrient matrix of ganoderma lucidum growth.
The present invention is prepared simply, and the lucid ganoderma stock culture speed of growth that culture medium spreads cultivation is fast, and growing way is vigorous, and switching contamination rate is low；
In addition, the sorghum rice boiling that parent species use reduces the row of production cost and pollutant also for the discarded object of pedigree seed culture medium
Put, the more significant efficiency and quality that improve ganoderma lucidum production.
（Four）Embodiment
The mother culture media that inventor is prepared using the present invention respectively carries out Mount Taishan purple sesame, Han Zhi, deer horn sesame and Wild ganoderma
Mother culture, certain embodiments are as follows：
Embodiment 1：
A kind of lucid ganoderma stock culture culture medium, including：Sorghum rice cooking water 1000mL, glucose 30g, peptone 3g, potassium dihydrogen phosphate
3g, magnesium sulfate 3g, agar 20g.
Culture medium preparation process：
The immersion of sorghum rice：Sorghum rice 500g, which is added water to, does not cross surface about 2cm, adds quick lime 5g, soaks 24h after mixing.
The boiling of sorghum rice：Sorghum rice is cleaned, drainage, be transferred in steamer, added water to and do not cross surface, slow boiling boiling
20min is full to sorghum rice expansion, and the sorghum grain of rice must not rupture.
The acquisition of sorghum rice filtrate：Boiling terminates, and the strainer filtering of grain of rice size is less than with eyelet, and with warm water 400mL
Sorghum rice is rinsed, gained filtrate merges.
The mixing of culture medium：Sorghum rice cooking liquor 1000mL is taken, adds glucose 30g, peptone 3g, potassium dihydrogen phosphate
3g, magnesium sulfate 3g, agar 20g, stirring, heating, moisturizing is to 1000mL after agar dissolves.
The packing of culture medium：Culture medium is sub-packed in test tube while hot（20×200 mm）In, liquid amount is no more than test tube height
1/3, when packing, prevents that culture medium adheres to test tube mouth, and packing is finished, sealed with silica gel plug, brown paper wrapping.
The sterilizing of culture medium and inclined-plane make：The test tube of wrapping is placed at 121 DEG C the 20min that sterilizes, puts inclined-plane, inclined-plane length
Degree control is in 9 ± 0.2cm, up to parent species test tube slant.
Mount Taishan purple sesame is accessed, mycelia can be covered with 12 days, compare control sample（Potato culture, that is, PDA culture medium）In advance 2
My god, mycelia is pure white, consolidation.
By this inclined-plane parent species（5×5 mm）Transfer into cotton seed hulls pedigree seed culture medium（Cotton seed hulls 88%, rice bran 10%, sucrose 1%,
Land plaster（CaSO4·2H2O）1%, moisture 58%）Afterwards, mycelia covers with Time transfer receiver in the same old way（PDA inclined-planes parent species switching cottonseed
Shell pedigree seed culture medium）The average time of mycelia shortens 2 days, and contamination rate declines 90%.
Embodiment 2：
A kind of lucid ganoderma stock culture culture medium, including：Sorghum rice cooking water 1000mL, glucose 30g, peptone 3g, potassium dihydrogen phosphate
3g, magnesium sulfate 3g, agar 20g.
Culture medium preparation process：
The immersion of sorghum rice：Sorghum rice 500g, which is added water to, does not cross surface about 2cm, adds quick lime 5g, soaks 24h after mixing.
The boiling of sorghum rice：Sorghum rice is cleaned, drainage, be transferred in steamer, added water to and do not cross surface, slow boiling boiling
20min is full to grain of rice expansion, and the sorghum grain of rice must not rupture.
The acquisition of sorghum rice filtrate：Boiling terminates, and the strainer filtering of grain of rice size is less than with eyelet, and with warm water 200mL
Sorghum rice is rinsed, gained filtrate merges.
The mixing of culture medium：Sorghum rice cooking liquor 1000mL is taken, adds glucose 20g, peptone 3g, potassium dihydrogen phosphate
3g, magnesium sulfate 3g, agar 30g, stirring, heating, moisturizing is to 1000mL after agar dissolves.
The packing of culture medium：Culture medium is sub-packed in test tube while hot（20×200mm）In, liquid amount is no more than test tube height
1/3, when packing, prevents that culture medium adheres to test tube mouth, and packing is finished, sealed with silica gel plug, brown paper wrapping.
The sterilizing of culture medium and inclined-plane make：The test tube of wrapping is placed at 105 DEG C the 30min that sterilizes, puts inclined-plane, inclined-plane length
Degree control is in 9 ± 0.2cm, up to parent species test tube slant.
Han Zhi is accessed, mycelia can be covered with 10 days, compare control sample（Potato culture）4 days in advance, mycelia was pure white, tight
It is real.
By this inclined-plane parent species（5×5 mm）Transfer into cotton seed hulls pedigree seed culture medium（Cotton seed hulls 88%, rice bran 10%, sucrose 1%,
Land plaster（CaSO4·2H2O）1%, moisture 58%）Afterwards, mycelia covers with Time transfer receiver in the same old way（PDA inclined-planes parent species switching cottonseed
Shell pedigree seed culture medium）The average time of mycelia shortens 4 days, and contamination rate declines 99%.
Embodiment 3：
A kind of lucid ganoderma stock culture culture medium, including：Sorghum rice cooking water 1000mL, glucose 30g, peptone 3g, potassium dihydrogen phosphate
3g, magnesium sulfate 3g, agar 20g.
Culture medium preparation process：
The immersion of sorghum rice：Sorghum rice 500g, which is added water to, does not cross surface about 2cm, adds quick lime 5g, soaks 24h after mixing.
The boiling of sorghum rice：Sorghum rice is cleaned, drainage, be transferred in steamer, added water to and do not cross surface, slow boiling boiling
20min, the sorghum grain of rice must not rupture.
The acquisition of sorghum rice filtrate：Boiling terminates, and the strainer filtering of grain of rice size is less than with eyelet, and with warm water 300mL
Sorghum rice is rinsed, gained filtrate merges.
The mixing of culture medium：Sorghum rice cooking liquor 1000mL is taken, adds glucose 30g, peptone 3g, potassium dihydrogen phosphate
3g, magnesium sulfate 3g, agar 20g, stirring, heating, moisturizing is to 1000mL after agar dissolves.
The packing of culture medium：Culture medium is sub-packed in test tube while hot（20×200mm）In, liquid amount is no more than test tube height
1/3, when packing, prevents that culture medium adheres to test tube mouth, and packing is finished, sealed with silica gel plug, brown paper wrapping.
The sterilizing of culture medium and inclined-plane make：The test tube of wrapping is placed at 121 DEG C the 20min that sterilizes, puts inclined-plane, inclined-plane length
Degree control is in 9 ± 0.2cm, up to parent species test tube slant.
Mount Taishan Wild ganoderma is accessed, mycelia can be covered with 11 days, compare control sample（Potato culture）3 days in advance, mycelia
Pure white, consolidation.
By this inclined-plane parent species（5×5mm）Transfer into cotton seed hulls pedigree seed culture medium（Cotton seed hulls 88%, rice bran 10%, sucrose 1%,
Land plaster（CaSO4·2H2O）1%, moisture 58%）Afterwards, mycelia covers with Time transfer receiver in the same old way（PDA inclined-planes parent species switching cottonseed
Shell pedigree seed culture medium）The average time of mycelia shortens 3 days, and contamination rate declines 80%.
Described above is not limitation of the present invention, and the invention is not restricted to examples detailed above.In the essential scope of the present invention,
The replacement of made simple change, remodeling or equal proportion, should also belong to protection scope of the present invention.
Claims (6)
1. a kind of lucid ganoderma stock culture culture medium, it is characterized in that, it is made of following raw material：Sorghum rice cooking water 1000mL, glucose 20-
40g, peptone 2-4g, potassium dihydrogen phosphate 2-4g, magnesium sulfate 2-4g, agar 15-25g.
2. lucid ganoderma stock culture culture medium according to claim 1, it is characterized in that, it is made of following raw material：Sorghum rice cooking water
1000mL, glucose 30g, peptone 3g, potassium dihydrogen phosphate 3g, magnesium sulfate 3g, agar 20g.
3. the preparation method of lucid ganoderma stock culture culture medium according to claim 1, it is characterized in that, include the following steps：（1）It is high
Fine strain of millet rice plus water and quick lime immersion, are transferred in steamer after rinsing afterwards, add water boiling, then filtered, flushing to obtain sorghum rice steaming
It is to boil water；（2）Sorghum rice cooking water 1000mL is taken, adds glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate and agar, stir,
Heating, moisturizing is to 1000mL after agar dissolves, and adjusts pH value；（3）Prepared culture medium is sub-packed in test tube, ox-hide
Paper bag is put into autoclave sterilizing after pricking, put inclined-plane, mother culture media test tube slant is remembered after cooling.
4. the preparation method of lucid ganoderma stock culture culture medium according to claim 1, it is characterised in that：Step（1）In, sorghum rice
500g, which is added water to, does not cross surface 2cm, adds quick lime, 24h is soaked after mixing, afterwards sorghum rice is cleaned with tap water, is transferred to
In steamer, adding water to and do not cross surface, slow boiling boiling 15-25min, boiling terminates, and the strainer filtering of grain of rice size is less than with eyelet,
With warm water washing and filter, merging filtrate again, obtain sorghum rice cooking water 1000mL.
5. the preparation method of lucid ganoderma stock culture culture medium according to claim 1, it is characterised in that：Step（1）In, after boiling
Sorghum rice filtering after, is rinsed and is filtered with 50-60 DEG C of warm water 200-500mL, twice gained filtrate merging.
6. the preparation method of lucid ganoderma stock culture culture medium according to claim 1, it is characterised in that：Step（3）In, prepare
Culture medium be sub-packed in while hot in test tube, liquid amount is no more than the 1/3 of test tube height, and when packing prevents that culture medium adheres to test tube
Mouthful, silica gel plug sealing, brown paper wrapping after packing, sterilize the 20min or 30min that sterilizes at 105 DEG C at 121 DEG C, pendulum
Inclined-plane, to obtain the final product.
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101971763A (en) * | 2010-03-22 | 2011-02-16 | 陈顺志 | Industrial energy-saving and low-carbon method for cultivating lucid ganoderma |
| CN102835254A (en) * | 2012-09-26 | 2012-12-26 | 烟台龙宇灵芝生物开发有限公司 | Ganoderma lucidum mycelia powder production process |
| CN103314788A (en) * | 2013-07-16 | 2013-09-25 | 龚涛 | Practical seafood mushroom culture medium and manufacturing method thereof |
| CN104082038A (en) * | 2014-07-10 | 2014-10-08 | 乳山市华隆生物科技有限公司 | Method for preparing high-purity lucid ganoderma hyphostroma powder through liquid submerged fermentation |
| CN104285820A (en) * | 2014-10-13 | 2015-01-21 | 海南森瑞谱生命科学药业股份有限公司 | Selenium-rich cordyceps militaris strain and application thereof |
| CN104956914A (en) * | 2015-06-18 | 2015-10-07 | 唐伟 | Breeding method for natural ganoderma lucidum |
| CN106747924A (en) * | 2016-11-22 | 2017-05-31 | 常州思宇知识产权运营有限公司 | A kind of method that sorghum rice prepares culture medium of edible fungus |
| CN107164236A (en) * | 2017-05-24 | 2017-09-15 | 贵州黔芝灵绿色农业科技发展有限公司 | A kind of preparation method of lucid ganoderma stock culture |
-
2017
- 2017-11-30 CN CN201711236476.5A patent/CN108029451A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101971763A (en) * | 2010-03-22 | 2011-02-16 | 陈顺志 | Industrial energy-saving and low-carbon method for cultivating lucid ganoderma |
| CN102835254A (en) * | 2012-09-26 | 2012-12-26 | 烟台龙宇灵芝生物开发有限公司 | Ganoderma lucidum mycelia powder production process |
| CN103314788A (en) * | 2013-07-16 | 2013-09-25 | 龚涛 | Practical seafood mushroom culture medium and manufacturing method thereof |
| CN104082038A (en) * | 2014-07-10 | 2014-10-08 | 乳山市华隆生物科技有限公司 | Method for preparing high-purity lucid ganoderma hyphostroma powder through liquid submerged fermentation |
| CN104285820A (en) * | 2014-10-13 | 2015-01-21 | 海南森瑞谱生命科学药业股份有限公司 | Selenium-rich cordyceps militaris strain and application thereof |
| CN104956914A (en) * | 2015-06-18 | 2015-10-07 | 唐伟 | Breeding method for natural ganoderma lucidum |
| CN106747924A (en) * | 2016-11-22 | 2017-05-31 | 常州思宇知识产权运营有限公司 | A kind of method that sorghum rice prepares culture medium of edible fungus |
| CN107164236A (en) * | 2017-05-24 | 2017-09-15 | 贵州黔芝灵绿色农业科技发展有限公司 | A kind of preparation method of lucid ganoderma stock culture |
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