CN108018209B - A kind of cell and bacterial strain culture device - Google Patents
A kind of cell and bacterial strain culture device Download PDFInfo
- Publication number
- CN108018209B CN108018209B CN201810130817.9A CN201810130817A CN108018209B CN 108018209 B CN108018209 B CN 108018209B CN 201810130817 A CN201810130817 A CN 201810130817A CN 108018209 B CN108018209 B CN 108018209B
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- Prior art keywords
- accommodating chamber
- pipe fitting
- led
- pipe
- chamber
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of cell and bacterial strain culture devices, including culture apparatus and attemperator, culture apparatus includes matrix A, the lower faces described matrix A are installed with support foot, accommodating chamber A is provided in described matrix A, it is provided with the screwed pipe in described matrix A in the accommodating chamber A appearance walls, the screwed pipe right-side outlet, which leads to be provided with, extends to the right external tube channel, the screwed pipe right end leads to the accommodating chamber A, the tube channel right side, which is led to, is provided with driven valve, the driven valve right side, which is led to, is provided with the first connecting leg, apparatus of the present invention are simple in structure, it is easy to use, this equipment is heated and is kept the temperature using immersion method, steady change amplitude is small, isoperibol can be provided to greatest extent, be conducive to cell and Spawn incubation.
Description
Technical field
The present invention relates to biological fields, and in particular to a kind of cell and bacterial strain culture device.
Background technology
The culture of cell and strain often requires and carries out at a certain temperature that traditional incubator needs directly to utilize
Electrothermal tube fever is to maintain the temperature in incubator, and the consumption energy is big and temperature is difficult to control, and temperature is easy excessively high or too low, temperature
Degree variation is violent, is unfavorable for the culture of cell and strain.
Invention content
The purpose of the present invention is to provide a kind of cell and bacterial strain culture devices, and the above-mentioned of the prior art can be overcome to lack
It falls into.
According to the present invention, a kind of cell and bacterial strain culture device of apparatus of the present invention, including culture apparatus and attemperator,
Culture apparatus includes matrix A, and the lower faces described matrix A are installed with support foot, and accommodating chamber A, the appearance are provided in described matrix A
It receives and is provided with the screwed pipe in described matrix A in chamber A appearance walls, the screwed pipe right-side outlet, which leads to be provided with, to extend to the right
External tube channel, the screwed pipe right end lead to the accommodating chamber A, and the tube channel right side, which is led to, is provided with driven valve,
The driven valve right side, which is led to, is provided with the first connecting leg, and the screwed pipe left side outlet, which leads to be provided with, extends to the left outside
Pipe fitting A, the pipe fitting A, which leads to left side to be provided with the left sides gate valve A, the gate valve A and lead to, is provided with pipe fitting B, described matrix A
Upper surface is installed with the storage case in the accommodating chamber A, and the storage case lower face is installed with and the lower ends the accommodating chamber A
Wall matched backing plate is provided with accommodating chamber B in the storage case, and the accommodating chamber B left end walls are installed with inductor, the appearance
Receive the metal tube for leading in chamber B lower end walls and being provided with and penetrating through the accommodating chamber A lower end walls and outside stretching out, the metal tube upper end
Face is installed with gate valve H, and the metal tube left and right sides is provided with the spring coil chamber in described matrix A, the spring coil chamber outer end wall
Inside be provided with and slide chamber, it is described slide that intracavitary can slide be provided with and slide bar, the bar that slides is installed with and institute towards inner face
The push plate that spring coil chamber or more end wall slides mating connection is stated, spring coil is provided between the push plate and the spring coil chamber outer end wall,
The push plate inward bottom wall is installed with fixing end, and the fixing end is matched with the fixing groove being set in the metal tube, institute
It states metal tube lower face and leads to be provided with the lower faces gate valve D, the gate valve D and lead to and be provided with soft tube, first connecting leg connects
It is connected to the right sides pipe fitting D, the pipe fitting D and leads to and be provided with electronic control valve, the electronic control valve right side, which is led to, to be provided with
Pipe fitting E, the pipe fitting E, which leads to right side, is provided with suction pump, and the suction pump right side, which is led to, is provided with downward extended pipe
It is provided with attemperator below part F, the pipe fitting F, attemperator includes the matrix B being fixedly arranged on ground, is set in described matrix B
It is equipped in accommodating chamber C, the accommodating chamber C and is provided with the suction head led to the pipe fitting F, it is solid in the accommodating chamber C inner bottom walls
Surely it is provided with heat-generating pipe, the heat-generating pipe is electrically connected to a power source.
Further technical solution, the screwed pipe are spiral, in accommodating chamber A appearance walls described in the screwed pipe winding, to
Improve the heat transfer efficiency of equipment.
The beneficial effects of the invention are as follows：
Apparatus of the present invention are simple in structure, easy to use, this equipment is heated and kept the temperature using immersion method, steady change width
It spends small, isoperibol can be provided to greatest extent, be conducive to cell and Spawn incubation.
Description of the drawings
It, below will be to embodiment or existing in order to illustrate more clearly of inventive embodiments or technical solution in the prior art
Attached drawing is briefly described needed in technology description, it should be apparent that, the accompanying drawings in the following description is only to invent
Some embodiments for those of ordinary skill in the art without creative efforts, can also basis
These attached drawings obtain other attached drawings.
Fig. 1 is culture apparatus structural schematic diagram in the present invention；
Fig. 2 is attemperator structural schematic diagram in the present invention.
Specific implementation mode
All features disclosed in this specification or disclosed all methods or in the process the step of, in addition to mutually exclusive
Feature and/or step other than, can combine in any way.
This specification（Including any accessory claim, abstract and attached drawing）Disclosed in any feature, except non-specifically chatting
It states, can be replaced by other alternative features that are equivalent or have similar purpose.That is, unless specifically stated, each feature is only
It is an example in a series of equivalent or similar characteristics.
As shown in Figs. 1-2, of the invention a kind of cell and bacterial strain culture device, including culture apparatus and attemperator, training
Foster device includes matrix A101, and the lower faces described matrix A101 are installed with support foot 100, receiving is provided in described matrix A101
Chamber A106, the screwed pipe 105 in described matrix A101 is provided in the accommodating chamber A106 appearance walls, and the screwed pipe 105 is right
Side outlet, which leads to be provided with, extends to the right external tube channel 122, and 105 right end of the screwed pipe is logical with the accommodating chamber A106
Even, 122 right side of the tube channel, which is led to, is provided with driven valve 121, and 121 right side of the driven valve, which is led to, is provided with the first company
Pipe 120,105 left side outlet of the screwed pipe, which leads to be provided with, extends to the left external pipe fitting A102, the pipe fitting A102 left ends
Face, which leads to be provided with the left sides gate valve A103, the gate valve A103 and lead to, is provided with pipe fitting B104, the upper surfaces described matrix A101
The storage case 107 being installed in the accommodating chamber A106,107 lower face of the storage case is installed with and the accommodating chamber
A106 lower end walls matched backing plate 112 is provided with accommodating chamber B110, the accommodating chamber B110 left ends in the storage case 107
Wall is installed with inductor 111, lead in the accommodating chamber B110 lower end walls be provided with the perforation accommodating chamber A106 lower end walls and
Metal tube 128 outside stretching out, 128 upper surface of the metal tube are installed with gate valve H113, the setting of 128 left and right sides of the metal tube
There is the spring coil chamber 126 in described matrix A101, is provided in 126 outer end wall of spring coil chamber and slides chamber 124, it is described to slide
Bar 125 is slided in being provided with for being slided in chamber 124, and the bar 125 that slides is installed with and about the 126 spring coil chamber towards inner face
End wall slides the push plate 123 of mating connection, and spring coil is provided between the push plate 123 and 126 outer end wall of spring coil chamber, described
123 inward bottom wall of push plate is installed with fixing end 127, the fixing end 127 and the fixing groove phase being set in the metal tube 128
Cooperation, 128 lower face of the metal tube, which leads to be provided with the lower faces gate valve D131, the gate valve D131 and lead to, is provided with soft tube
129, first connecting leg 120, which is connected to the right sides pipe fitting D115, the pipe fitting D115 and leads to, is provided with electronic control valve 116,
116 right side of the electronic control valve, which leads to be provided with the right sides pipe fitting E117, the pipe fitting E117 and lead to, is provided with suction pump
118,118 right side of the suction pump, which is led to, is provided with downward extended pipe fitting F119, and guarantor is provided with below the pipe fitting F119
Warm device, attemperator include the matrix B136 being fixedly arranged on ground, and accommodating chamber C137 is provided in described matrix B136, described
It is provided with the suction head 144 led to the pipe fitting F119 in accommodating chamber C137, fixes and sets in the accommodating chamber C137 inner bottom walls
It is equipped with heat-generating pipe 135, the heat-generating pipe 135 is electrically connected to a power source.
Valuably, wherein the screwed pipe 105 is spiral, in accommodating chamber A106 appearance walls described in 105 winding of the screwed pipe,
To improve the heat transfer efficiency of equipment.
In the initial state, the gate valve A, electronic control valve, driven valve, gate valve D and gate valve H are in close present device
Closed state, the suction pump are in stop working state, to be conducive to the maintenance and installation of equipment.
When equipment is run, the gate valve A, electronic control valve 116 and driven valve are opened, and the heat-generating pipe 135 is powered, right
Water in the accommodating chamber C137 is heated, and water is sucked through pipe fitting F119, suction pump 118, pipe by the suction head 144
Part E117, pipe fitting D115, first connecting leg 120, the tube channel 122 enter the accommodating chamber A106 and are full of the screwed pipe
105, heat is transmitted to by the accommodating chamber A in the accommodating chamber B, and the accommodating chamber B is heated and kept the temperature, the induction
The temperature that device perceives in the accommodating chamber B is controlled to facilitate, to carry out the training of cell and strain for the accommodating chamber B
Support the environment that constant temperature is provided；
When the water for needing to be discharged in the accommodating chamber A106, the gate valve A103 is opened, the accommodating chamber A106 and described
Water in screwed pipe 105 is discharged from pipe fitting A；
Carry out cell and when Spawn incubation when directly filling liquid in accommodating chamber B110, culture terminate to open gate valve H113 and
Liquid is discharged in the gate valve D131.
The beneficial effects of the invention are as follows：
Apparatus of the present invention are simple in structure, easy to use, this equipment is heated and kept the temperature using immersion method, steady change width
It spends small, isoperibol can be provided to greatest extent, be conducive to cell and Spawn incubation.
The above, the specific implementation mode only invented, but invent protection domain be not limited thereto, it is any without
The change or replacement that creative work is expected are crossed, should all be covered within the protection domain of invention.Therefore, the protection domain of invention
It should be determined by the scope of protection defined in the claims.
Claims (2)
1. a kind of cell and bacterial strain culture device, including culture apparatus and attemperator, culture apparatus includes matrix A, the base
The lower faces body A are installed with support foot, and accommodating chamber A is provided in described matrix A, is provided with and is located in the accommodating chamber A appearance walls
Screwed pipe in described matrix A, the screwed pipe right-side outlet, which leads to be provided with, extends to the right external tube channel, and the screwed pipe is right
End is led to the accommodating chamber A, and the tube channel right side, which is led to, is provided with driven valve, and setting is led in the driven valve right side
There is the first connecting leg, the screwed pipe left side outlet, which is led to, is provided with that extend the external left sides pipe fitting A, the pipe fitting A to the left logical
It is connected with to be equipped with the left sides gate valve A, the gate valve A and lead to and is provided with pipe fitting B, the upper surfaces described matrix A are installed with positioned at the appearance
The storage case received in chamber A, the storage case lower face are installed with and the matched backing plate of accommodating chamber A lower end walls, the appearance
It receives and is provided with accommodating chamber B in case, the accommodating chamber B left end walls are installed with inductor, lead to setting in the accommodating chamber B lower end walls
There is the metal tube penetrated through outside the accommodating chamber A lower end walls and stretching, the metal tube upper surface is installed with gate valve H, the gold
Belong to pipe left and right sides and be provided with the spring coil chamber in described matrix A, is provided in the spring coil chamber outer end wall and slides chamber, the cunning
Bar is slided in being provided with for being slided in an actor's rendering of an operatic tune, it is described slide bar and be installed with to slide with end wall above and below the spring coil chamber towards inner face match
The push plate for closing connection, is provided with spring coil, the push plate inward bottom wall is installed between the push plate and the spring coil chamber outer end wall
Fixing end, the fixing end are matched with the fixing groove being set in the metal tube, and setting is led in the metal tube lower face
There are the lower faces gate valve D, the gate valve D to lead to and be provided with soft tube, first connecting leg is connected to pipe fitting D, the pipe fitting D right ends
Face, which is led to, is provided with electronic control valve, and the electronic control valve right side leads to that be provided with the right sides pipe fitting E, the pipe fitting E logical
It is connected with and is equipped with suction pump, the suction pump right side, which is led to, is provided with downward extended pipe fitting F, is provided with below the pipe fitting F
Attemperator, attemperator include the matrix B being fixedly arranged on ground, and accommodating chamber C, the accommodating chamber C are provided in described matrix B
It is inside provided with the suction head led to the pipe fitting F, heat-generating pipe, the fever are fixedly installed in the accommodating chamber C inner bottom walls
Pipe is electrically connected to a power source.
2. a kind of cell as described in claim 1 and bacterial strain culture device, it is characterised in that：The screwed pipe is spiral, described
In accommodating chamber A appearance walls described in screwed pipe winding.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810130817.9A CN108018209B (en) | 2018-02-08 | 2018-02-08 | A kind of cell and bacterial strain culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810130817.9A CN108018209B (en) | 2018-02-08 | 2018-02-08 | A kind of cell and bacterial strain culture device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108018209A CN108018209A (en) | 2018-05-11 |
| CN108018209B true CN108018209B (en) | 2018-09-28 |
Family
ID=62075346
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810130817.9A Expired - Fee Related CN108018209B (en) | 2018-02-08 | 2018-02-08 | A kind of cell and bacterial strain culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108018209B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117801954A (en) * | 2024-01-08 | 2024-04-02 | 山东诺心生物科技有限公司 | Bionic culture device for embryonic stem cells |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3980131A (en) * | 1975-05-08 | 1976-09-14 | New Brunswick Scientific Co., Inc. | Sterilizer for culture media and laboratory ware |
| CN201729836U (en) * | 2010-07-14 | 2011-02-02 | 中国人民解放军第四军医大学 | Cell culture dish convenient for perfusion and heat insulation |
| CN201746538U (en) * | 2010-08-02 | 2011-02-16 | 格特生物制药（天津）有限公司 | Compound-bacteria mixed cultivating device |
| CN203065486U (en) * | 2013-01-22 | 2013-07-17 | 桂林电子科技大学 | Device for rapid enrichment-proliferation and purification-cultivation for anaerobic ammonium oxidation bacteria |
| CN204482428U (en) * | 2015-02-17 | 2015-07-22 | 湖南和平生物科技有限公司 | A kind of strain cultivation tank |
| CN206279212U (en) * | 2016-12-01 | 2017-06-27 | 广州美岸生物科技有限公司 | A kind of cell culture apparatus |
| CN206308374U (en) * | 2016-12-30 | 2017-07-07 | 宁波键一生物科技有限公司 | A kind of fully-automatic intelligent cell culture apparatus |
| CN206736259U (en) * | 2017-05-03 | 2017-12-12 | 天津鸿泽天创生物环保科技发展有限公司 | A kind of automatic manufacturing device for being adapted to a large amount of probiotics fermentions |
| CN206927896U (en) * | 2017-06-07 | 2018-01-26 | 塔里木大学 | A kind of special Prevention of Infectious Diseases thermostatic type strain culturing device of animal doctor |
-
2018
- 2018-02-08 CN CN201810130817.9A patent/CN108018209B/en not_active Expired - Fee Related
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3980131A (en) * | 1975-05-08 | 1976-09-14 | New Brunswick Scientific Co., Inc. | Sterilizer for culture media and laboratory ware |
| CN201729836U (en) * | 2010-07-14 | 2011-02-02 | 中国人民解放军第四军医大学 | Cell culture dish convenient for perfusion and heat insulation |
| CN201746538U (en) * | 2010-08-02 | 2011-02-16 | 格特生物制药（天津）有限公司 | Compound-bacteria mixed cultivating device |
| CN203065486U (en) * | 2013-01-22 | 2013-07-17 | 桂林电子科技大学 | Device for rapid enrichment-proliferation and purification-cultivation for anaerobic ammonium oxidation bacteria |
| CN204482428U (en) * | 2015-02-17 | 2015-07-22 | 湖南和平生物科技有限公司 | A kind of strain cultivation tank |
| CN206279212U (en) * | 2016-12-01 | 2017-06-27 | 广州美岸生物科技有限公司 | A kind of cell culture apparatus |
| CN206308374U (en) * | 2016-12-30 | 2017-07-07 | 宁波键一生物科技有限公司 | A kind of fully-automatic intelligent cell culture apparatus |
| CN206736259U (en) * | 2017-05-03 | 2017-12-12 | 天津鸿泽天创生物环保科技发展有限公司 | A kind of automatic manufacturing device for being adapted to a large amount of probiotics fermentions |
| CN206927896U (en) * | 2017-06-07 | 2018-01-26 | 塔里木大学 | A kind of special Prevention of Infectious Diseases thermostatic type strain culturing device of animal doctor |
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| Publication number | Publication date |
|---|---|
| CN108018209A (en) | 2018-05-11 |
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| TA01 | Transfer of patent application right |
Effective date of registration: 20180816 Address after: 200120 C, 888, west two road, Nanhui new town, Pudong New Area, Shanghai Applicant after: IMPROVING LIFE BIOTECHNOLOGY (SHANGHAI) CO., LTD. Address before: 312399 Shaoxing, Shangyu, Zhejiang Shangyu District, the new village village street, Buddha Hall piece No. 3 Applicant before: Shangyu Shaoxing Zhongtai medical science and Technology Co., Ltd. |
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