CN108002541A - A kind of decolorising agent containing elm mushroom bacteria residue extract - Google Patents
A kind of decolorising agent containing elm mushroom bacteria residue extract Download PDFInfo
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- CN108002541A CN108002541A CN201711167402.0A CN201711167402A CN108002541A CN 108002541 A CN108002541 A CN 108002541A CN 201711167402 A CN201711167402 A CN 201711167402A CN 108002541 A CN108002541 A CN 108002541A
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- residue
- mushroom
- bacteria residue
- decolorising agent
- culture
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Classifications
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D3/00—Calcareous fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/308—Dyes; Colorants; Fluorescent agents
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- Inorganic Chemistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Pest Control & Pesticides (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
- Mushroom Cultivation (AREA)
Abstract
The present invention relates to a kind of decolorising agent containing elm mushroom bacteria residue extract, the decolorising agent is prepared by the following method：1) elm mushroom bacteria residue is extracted with citrate buffer solution, bacteria residue residue is obtained after removing extracting solution；2) flat mushroom strain is inoculated with bacteria residue residue, fermented and cultured is carried out, the mix products of culture medium and mycelia is extracted with citrate buffer solution after culture, obtain decolorising agent.Cultivated present invention discover that oyster mushroom is placed on Pleurotus citrinopileatus bacteria residue, the extract of gained has higher laccase activity and preferable decolorizing effect；And the raw material of decolorising agent of the present invention is elm mushroom bacteria residue, the comprehensive recycling of discarded object is advantageously implemented, reduces production cost.
Description
Technical field
The present invention relates to the recycling field of elm mushroom bacteria residue, it is specifically related to a kind of containing elm mushroom bacteria residue extract
Decolorising agent.
Background technology
In recent years, dyestuff due to it is cheap, to illumination, temperature and detergent equistability is strong and color is various, by
It is widely used in textile and toner manufacturing industry etc. (Asgher et al, 2008).With developing rapidly for textile industry,
The kind and quantity of dyestuff are continuously increased, oneself warp of dyeing becomes water resources consumption person and the pollution of China's natural water maximum
One of source.And it is dye decolored be waste water from dyestuff administer key issue.The method of processing waste water from dyestuff mainly has physical-chemical process
With two class of biological methods.Biological method possesses the features such as continuation degradation capability is strong, environmental-friendly, adaptable, thus by
To extensive concern.Wherein white-rot fungi can a variety of extracellular enzymes such as secretion laccase, lignin peroxidase, manganese peroxidase etc.
Feature, is expected to obtain large-scale application in terms of wastewater treatment.
With the fast development of China's mushroom industry, eat too much bacterium bacteria residue and constantly produce, but the comprehensive profit of bacteria residue
Relatively low with rate, most of bacteria residue is dropped, and is on the one hand caused environmental pollution, miscellaneous bacteria and pathogen is on the other hand grown, to food
Security risk is brought with bacterium scale producing region.Research finds that biological prosthetic effect of the edible mushroom to dyestuff contaminant has from it
Two basic functions having：Biodegradable and biological concentration.With it is biodegradable because itself with it is a variety of can degrade it is organic
The lignin enzyme of pollutant, in addition, special physiological mechanism possessed by edible mushroom allows them to the richness from the environment of growth
Collection trace element.And edible fungi residue contains a large amount of mycelia, therefore, edible fungi residue has in the biological prosthetic field of dye discoloration
There is very big application potential.
The content of the invention
The object of the present invention is to provide a kind of decolorising agent containing elm mushroom bacteria residue extract, the decolorising agent is by such as lower section
Method is prepared：
1) elm mushroom bacteria residue is extracted with citrate buffer solution, bacteria residue residue is obtained after removing extracting solution；
2) flat mushroom strain is inoculated with bacteria residue residue, fermented and cultured is carried out, with citrate buffer solution pair after culture
Culture medium and the mix products of mycelia are extracted, and obtain decolorising agent.
Cultivated it is a discovery of the invention that oyster mushroom is placed on the remaining bacteria residue after elm mushroom is extracted with citrate buffer solution,
Finally there is good decolorizing effect with the mixture after citrate buffer solution extraction culture, the extracting solution of gained again so that elm
Yellow mushroom bacteria residue has obtained further efficiently using.
Preferably, the elm mushroom bacteria residue is elm mushroom culture 22~25 days, harvests the culture medium residue after mushroom；Elm
The culture medium of yellow mushroom forms, by weight, including 56~60 parts of bluish dogbane bar, 18~22 parts of weed tree sawdust, 18~22 parts of wheat bran, stone
0.5~1.5 part of cream, 0.5~1.5 part of lime.
Common culture medium is in actual production, by weight, including 58 parts of bluish dogbane bar, 20 parts of weed tree sawdust, 20 parts of wheat bran,
1 part of gypsum, 1 part of lime.
Preferably, in the step 1), use pH to be extracted for 4.8 citrate buffer solution to elm mushroom bacteria residue, carry
The mass volume ratio 1 of elm mushroom bacteria residue and citrate buffer solution during taking:3~6.
Preferably, in the step 1), the concrete operations of extraction are, by the mixture of elm mushroom bacteria residue and citric acid 26
Under conditions of~30 DEG C, 3~5 hours are shaken in 180~200rpm shaking tables, gained lower part is precipitated as bacteria residue residue after centrifugation
Thing.
Preferably, need to pre-process the bacteria residue residue before flat mushroom strain is inoculated with the step 2), it is described
The operation of pretreatment is to add distilled water thereto after the bacteria residue residue is dried, and is 50~65% to its water content.
Preferably, the condition of the oyster mushroom fermentation culture is 18~24d of quiescent culture under conditions of 27~30 DEG C.
It is further preferred that the condition of the oyster mushroom fermentation culture is 20~22d of quiescent culture under conditions of 27~30 DEG C.
Oyster mushroom can produce different metabolites in the different times of growth, and the metabolite enzyme activity when cultivating 18~24d is stronger, table
Reveal more preferable decoloring ability, effect is more preferable when cultivating 20~22d.
Preferably, the inoculum concentration of oyster mushroom is 10%-15% in the solid medium.Above-mentioned percentage is calculated as quality
The flat mushroom strain after 10~15mL activation is added in volume ratio, the i.e. culture medium per 100g.
Preferably, the activation method of the flat mushroom strain is that stored refrigerated flat mushroom strain is pressed inoculum concentration 10%-15%
It is inoculated into the basal medium of 100mL, under conditions of 27~29 DEG C, with the rotating speed activation culture 5d of 140~160rpm；Institute
The composition for stating basal medium is：Glucose 20g/L, yeast extract 5g/L, KH2PO4 1g/L、MgS040.5g/L, vitamin
B10.010g/L。
Preferably, extraction operation is in the step 2), by culture medium and the mix products of mycelia and lemon that pH is 4.8
Acid buffer presses mass volume ratio 1:7~9 ratio is mixed, and 120~150rpm shakes under 18~22 DEG C of temperature conditionss
3~5h of extraction is swung, is centrifuged after filtering, supernatant is decolorising agent.
Preferably, the present processes include the following steps：
1) elm mushroom bacteria residue and pH are delayed by mass volume ratio 1 for 4.8 citric acid:3~5 mixing, then 26~30
Under conditions of DEG C, 3~5 hours are shaken in 180~200rpm shaking tables, elm mushroom bacteria residue is extracted, after centrifugation under gained
Portion is precipitated as bacteria residue residue；
The elm mushroom bacteria residue is elm mushroom culture 22~25 days, harvests the culture medium residue after mushroom；The elm is yellow
Mushroom is cultivated on following culture medium, by weight, including 56~60 parts of bluish dogbane bar, 18~22 parts of weed tree sawdust, wheat bran 18~
22 parts, 0.5~1.5 part of gypsum, 0.5~1.5 part of lime；
2) distilled water is added thereto after the bacteria residue residue is dried, and is 50~65% to its water content, wherein
It is inoculated with flat mushroom strain, 20~22d of quiescent culture under conditions of 27~30 DEG C, by the mixing of culture medium and mycelia after culture
The citrate buffer solution of product and pH4.8 press mass volume ratio 1:7~9 ratio is mixed, in 18~22 DEG C of temperature strip
3~5h of mechanical shaking extraction under part, is centrifuged after filtering, and supernatant is decolorising agent.
Flat mushroom strain used in the present invention is oyster cap fungus (bio-67015), is inquired about by Chinese microorganism strain
Net purchase can buy.
The buffer solution of pH4.8 used in the present invention is prepared into after being mixed by the solution of citric acid and trisodium citrate
Arrive.
The method of the present invention has the advantages that：
1) cultivated present invention discover that oyster mushroom is placed on Pleurotus citrinopileatus bacteria residue, the extract of gained has higher laccase
Active and preferable decolorizing effect；
3) raw material of decolorising agent of the present invention is elm mushroom bacteria residue, is advantageously implemented the comprehensive recycling of discarded object, drop
Low production cost.
3) operating method of the present invention is simple, makes raw material is easy to get, and cost is low, is mass produced suitable for batch production.
Embodiment
Following embodiments are used to illustrate the present invention, but are not limited to the scope of the present invention.
Flat mushroom strain involved in embodiment is oyster cap fungus (bio-67015), and net is inquired about by Chinese microorganism strain
It is commercially available.
Involved flat bacterium activates to obtain by the following method in embodiment, and stored refrigerated flat mushroom strain is pressed inoculum concentration
10%-15% is inoculated into the basal medium of 100mL, under conditions of 28 DEG C, with the rotating speed activation culture 5d of 150rpm, is obtained
Nutrient solution after activation.The composition of the basal medium is：Glucose 20g/L, yeast extract 5g/L, KH2PO4 1g/L,
MgS040.5g/L, vitamin B10.010g/L。
Embodiment 1
The present embodiment is related to a kind of preparation method of decolorising agent, includes the following steps：
1) elm mushroom bacteria residue and pH are delayed by mass volume ratio 1 for 4.8 citric acid:4 mixing, then in 28 DEG C of condition
Under, 4 hours are shaken in 190rpm shaking tables, elm mushroom bacteria residue is extracted, gained lower part is precipitated as bacteria residue residue after centrifugation
Thing；
The elm mushroom bacteria residue is elm mushroom culture 22~25 days, harvests the culture medium residue after mushroom；The elm is yellow
Mushroom is cultivated on following culture medium, by weight, including 58 parts of bluish dogbane bar, 20 parts of weed tree sawdust, 20 parts of wheat bran, 1 part of gypsum,
1 part of lime；
2) distilled water is added thereto after the bacteria residue residue is dried, and is 50~65% to its water content, wherein
Be inoculated with flat mushroom strain, the quiescent culture 21d under conditions of 28 DEG C, after culture by culture medium and the mix products of mycelia with
The citrate buffer solution of pH4.8 presses mass volume ratio 1:8 ratio is mixed, and 140rpm vibrates under 20 DEG C of temperature conditionss
4h is extracted, 5000r/min centrifuges 5min after filtering, takes supernatant, is decolorising agent.
Embodiment 2
Compared with Example 1, its difference lies in by the oyster mushroom fermentation culture 19d in the step 2).
Comparative example 1
Compared with Example 1, its difference lies in cultivated oyster mushroom without using elm mushroom bacteria residue, commonly used using oyster mushroom
Culture medium PDA culture medium potato (peeling) 200g, glucose 20g, distilled water 1000ml, natural pH is under the same conditions
Oyster mushroom is cultivated, then the product after culture is extracted using identical method.
Comparative example 2
Compared with Example 1, its difference lies in, without step 1) to elm mushroom bacteria residue extract after remove extracting solution behaviour
Make, directly oyster mushroom is cultivated using elm mushroom bacteria residue.Distilled water is added in bacteria residue, is 50~65% to its water content,
Flat mushroom strain is wherein inoculated with, the quiescent culture 21d under conditions of 28 DEG C, by culture medium and the mix products of mycelia after culture
Mass volume ratio 1 is pressed with the citrate buffer solution of pH4.8:8 ratio is mixed, and 140rpm shakes under 20 DEG C of temperature conditionss
Extraction 4h is swung, 5000r/min centrifuges 5min after filtering, takes supernatant.
Comparative example 3
Compared with Example 1, its difference lies in be 14d to the cultivated days of oyster mushroom in step 2).
Comparative example 4
Compared with Example 1, its difference lies in be 28d to the cultivated days of oyster mushroom in step 2).
Experimental example
The correlated performance of embodiment 1~4 and comparative example 1~4 is tested.
The measure of enzymatic activity：
Experimental method：Laccase activity assay method：Accurate malonic acid-sodium malonate the buffer solution for pipetting 100mmol/L,
Each 10ml of ABTS solution of 0.6mmol/L, mixing, shakes up, 30 DEG C of water-bath 5min；Mixed liquor 200ul is taken in ELISA Plate hole,
Return to zero at 420nm；The accurate decolorising agent 10ul added in embodiment and comparative example, immediate record light absorption value, records every 1min
Once, totally 3 times, it is averaged, is converted to absorbance change (OD per minute420Change).
Indigo decolorization experiment：Take the indigo solution of 3 parts of 10ml 100mg/L, add 2ml2.5g/L HBT solution and
The decolorising agent of 20ml embodiments and comparative example, is placed in 30 DEG C, in 70r/min shaking baths, a light absorption value is surveyed when 2 is small.
Congo red decolorization experiment：Take the Congo red solution of 3 parts of 10ml 100mg/L, and 20ml embodiments and comparative example
Decolorising agent, is placed in 30 DEG C, in 70r/min shaking baths, a light absorption value is surveyed when 2 is small.
Acquired results such as table 1：
Although above the present invention is made to retouch in detail with general explanation, embodiment and experiment
State, but on the basis of the present invention, it can be made some modifications or improvements, this is apparent to those skilled in the art
's.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to claimed
Scope.
Claims (9)
1. a kind of decolorising agent containing elm mushroom bacteria residue extract, it is characterised in that the decolorising agent is prepared into by the following method
Arrive：
1) elm mushroom bacteria residue is extracted with citrate buffer solution, bacteria residue residue is obtained after removing extracting solution；
2) flat mushroom strain is inoculated with the bacteria residue residue, fermented and cultured is carried out, with citrate buffer solution pair after culture
Culture medium and the mix products of mycelia are extracted, and obtain decolorising agent.
2. decolorising agent according to claim 1, it is characterised in that the elm mushroom bacteria residue is elm mushroom culture 22~25
My god, harvest the culture medium residue after mushroom；The culture medium of elm mushroom forms, by weight, including 56~60 parts of bluish dogbane bar,
18~22 parts of weed tree sawdust, 18~22 parts of wheat bran, 0.5~1.5 part of gypsum, 0.5~1.5 part of lime.
3. decolorising agent according to claim 1, it is characterised in that in the step 1), use pH to delay for 4.8 citric acid
Fliud flushing extracts elm mushroom bacteria residue, the mass volume ratio 1 of elm mushroom bacteria residue and citrate buffer solution in extraction process:3~
6。
4. the decolorising agent according to claim 1 or 3, it is characterised in that in the step 1), the concrete operations of extraction are,
Shaken by the mixture of elm mushroom bacteria residue and citric acid under conditions of 26~30 DEG C, in 180~200rpm shaking tables 3~5 small
When, gained lower part is precipitated as bacteria residue residue after centrifugation.
5. the decolorising agent according to claim 1 or 4, it is characterised in that needed in the step 2) before flat mushroom strain is inoculated with
The bacteria residue residue is pre-processed, the operation of the pretreatment is to be added thereto after the bacteria residue residue is dried
Distilled water, is 50~65% to its water content.
6. decolorising agent according to claim 1 or 5, it is characterised in that the condition of the oyster mushroom fermentation culture be 27~
18~24d of quiescent culture under conditions of 30 DEG C, it is preferred that 20~22d of culture.
7. decolorising agent according to claim 6, it is characterised in that the inoculum concentration of flat mushroom strain is in the solid medium
10%-15%；
And/or the activation method of the flat mushroom strain is to be inoculated into stored refrigerated flat mushroom strain by inoculum concentration 10%-15%
In the basal medium of 100mL, under conditions of 27~29 DEG C, with the rotating speed activation culture 5d of 140~160rpm；The basis
The composition of culture medium is：Glucose 20g/L, yeast extract 5g/L, KH2PO4 1g/L、MgS040.5g/L, vitamin
B10.010g/L。
8. the decolorising agent according to claim 1 or 6, it is characterised in that extraction operation is in the step 2), by culture medium
With the mix products of mycelia mass volume ratio 1 is pressed for 4.8 citrate buffer solution with pH:7~9 ratio is mixed, 18
120~150rpm mechanical shaking extractions, 3~5h under~22 DEG C of temperature conditionss, is centrifuged after filtering, and supernatant is decolorising agent.
9. decolorising agent according to claim 1, it is characterised in that be prepared by the following method：
1) elm mushroom bacteria residue and pH are delayed by mass volume ratio 1 for 4.8 citric acid:3~5 mixing, then at 26~30 DEG C
Under the conditions of, 3~5 hours are shaken in 180~200rpm shaking tables, elm mushroom bacteria residue is extracted, gained lower part sinks after centrifugation
Form sediment for bacteria residue residue；
The elm mushroom bacteria residue is elm mushroom culture 22~25 days, harvests the culture medium residue after mushroom；The elm mushroom exists
Cultivated on following culture medium, by weight, including 56~60 parts of bluish dogbane bar, 18~22 parts of weed tree sawdust, 18~22 parts of wheat bran,
0.5~1.5 part of gypsum, 0.5~1.5 part of lime；
2) distilled water is added thereto after the bacteria residue residue is dried, and is 50~65% to its water content, is inoculated with wherein
Flat mushroom strain, 20~22d of quiescent culture under conditions of 27~30 DEG C, by culture medium and the mix products of mycelia after culture
Mass volume ratio 1 is pressed with the citrate buffer solution of pH4.8:7~9 ratio is mixed, under 18~22 DEG C of temperature conditionss
3~5h of mechanical shaking extraction, is centrifuged after filtering, and supernatant is decolorising agent.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711167402.0A CN108002541B (en) | 2017-11-21 | 2017-11-21 | A decolorizing agent containing Pleurotus Citrinopileatus Sing fungus residue extract |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711167402.0A CN108002541B (en) | 2017-11-21 | 2017-11-21 | A decolorizing agent containing Pleurotus Citrinopileatus Sing fungus residue extract |
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| CN108002541B CN108002541B (en) | 2020-06-23 |
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| CN107267473A (en) * | 2017-06-29 | 2017-10-20 | 商丘师范学院 | The method that laccase is extracted from pleurotus eryngii bacteria residue |
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2017
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|---|---|---|---|---|
| JP2006281042A (en) * | 2005-03-31 | 2006-10-19 | Suminoe Textile Co Ltd | Continuous porous molded body immobilizing microbe and method for discoloring dye using this |
| CN104445636A (en) * | 2014-11-11 | 2015-03-25 | 福建农林大学 | Method for treating polluted wastewater by virtue of pleurotus eryngii cultivation material |
| CN104495971A (en) * | 2014-11-27 | 2015-04-08 | 福建农林大学 | Method for processing aniline-blue-containing pollution wastewater by using cultivation material of harvested pleurotus eryngii |
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| Title |
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| 杨清香 等: "平菇漆酶的性质和应用研究", 《环境科学与技术》 * |
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