CN107988101A - A kind of complex microorganism preparations and preparation method thereof - Google Patents
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The present invention relates to a kind of complex microorganism preparations and preparation method thereof, it includes following weight percent composition：Fermentation material 45-55%, fish meal 8-12%, rice bran 15-25%, soybean protein 4-6%, starch 12-18%；Fermentation material includes following components in percentage by weight：Calcium carbonate 35%, bacillus subtilis bacterium powder 1%, probiotics bacterium powder 1%, tapioca starch 5%, soy meal 10%, carbohydrate 3%, yeast 2.4%, composite mineral salt 0.6%, water 42%；Probiotics bacterium powder includes following components：Lactobacillus acidophilus bacterium powder, bifidobacterium longum bacterium powder, Lactobacillus casei bacterium powder.The complex microorganism preparations of the present invention are widely used, can be used for the growth for suppressing pathogenic bacteria, can be used for ammonia nitrogen in degradation water, nitrite, decompose the organic matters such as the excrement in pond, the complex microorganism preparations are formed by natural material fermentation, and no added antibiotic etc is pollution-free to water body.
Description
Technical field
The present invention relates to complex microorganism preparations technical field, and in particular to a kind of complex microorganism preparations and its preparation side
Method.
Background technology
In recent years, China prawn culture fishery was grown rapidly, and intensive, high-density breeding scale expands day by day, and was collected
Middle bait throwing in causes to accumulate substantial amounts of residual bait, excrement and animals and plants corpse in prawn culturing water body, causes water body nitrogen element content tight
Weight is exceeded, eutrophication aggravation so that breeding ecological environment is destroyed, and shrimp disease takes place frequently, and ultimately causes huge economy
Loss.With the highdensity development of aquaculture, daily ration, feeding quantity persistently rises, and denitrifying load continues to increase in nature, causes ammonia
The accumulation of nitrogen and nitrite and blue algae increase, cause harmful bacteria in water body to increase.Research shows, ammonia nitrogen, nitrite,
It is main contributor that aquatic livestock is caused a disease that cyanobacteria, harmful bacteria, which increase, and ammonia nitrogen and content of nitrite increase in water body, cause to support
Object anoxic is grown, cyanobacteria and harmful bacteria increase, and then trigger various diseases.So ammonia nitrogen and nitrous in control breeding water body
Hydrochlorate becomes the emphasis of aquaculture water quality processing, and the difficult point of water treatment at present.Due to bio-denitrification technology have into
This is low, easy to operate, qualified discharge is highly reliable and the advantages that non-secondary pollution, is increasingly becoming trade effluent, sanitary sewage and water
The major way of aquaculture waste processing.Prepare the ammonia nitrogen in a kind of excellent complex microorganism preparations degrading cultivation water and Asia
Nitrate, has great significance for prawn aquaculture.
The content of the invention
Object of the present invention is to provide a kind of complex microorganism preparations, ammonia nitrogen that can be effectively in degrading cultivation water and
Nitrite, improves aquaculture water quality.
The present invention also aims to provide the preparation method of above-mentioned complex microorganism preparations, which can
Lean on, cost is low, it is possible to achieve large-scale production.
First purpose of the present invention is realized by following technical solution：A kind of complex microorganism preparations, its
Include following components in percentage by weight：Fermentation material 45-55%, fish meal 8-12%, rice bran 15-25%, soybean protein 4-6%,
Starch 12-18%.
Preferably, the fermentation material 50%, fish meal 10%, rice bran 20%, soybean protein 5%, starch 15%.
Further, the fermentation material includes following components in percentage by weight：Calcium carbonate 35%, bacillus subtilis bacterium
Powder 1%, probiotics bacterium powder 1%, tapioca starch 5%, soy meal 10%, carbohydrate 3%, yeast 2.4%, composite mineral salt 0.6%, water
42%.
Further, the probiotics bacterium powder includes following components：Lactobacillus acidophilus bacterium powder, bifidobacterium longum bacterium powder, do
Lactobacillus paracasei bacterium powder；Preferably, the lactobacillus acidophilus bacterium powder, bifidobacterium longum bacterium powder, Lactobacillus casei bacterium powder weight hundred
Divide than being 30%：30%：40%.
The major microorganisms flora used in the present invention include bacillus subtilis, lactobacillus acidophilus in probiotics bacterium powder,
Bifidobacterium longum, Lactobacillus casei, these complex microorganism bacterial strain ratios go to purify water using single microorganism strain to be had more preferably
Synergistic effect, the comprehensive functions such as oxidation, ammonification, nitrosation, nitrification, vulcanization, fixed nitrogen can be played, can secrete ectoenzyme element,
Eliminate organic matter, the NH in water body3、H2The harmful substances such as S, reduce chemical oxygen consumption (COC) (COD) and biological oxygen consumption (BOD), indirectly
Increase the dissolved oxygen content in water body, improve Water quality.
Second object of the present invention is achieved through the following technical solutions, the preparation of above-mentioned complex microorganism preparations
Method, comprises the following steps：
Step S1, the preparation of fermentation material：Fermentation material component is stirred mixing according to the amount of percentage by weight, mixing is equal
After even, put to fermentation cask fermentation, fermenter is placed in fermenting house during fermentation；
Step S2, the preparation of mixed fermentation material：By the fermentation material fermented, fish meal, rice bran, soybean protein and starch by weight
The amount of amount percentage is sufficiently mixed；
Step S3, the preparation of semi-finished product：Mixed fermentation material is dispensed, subsequently into baking room, semi-finished product moisture contains
Measuring up to standard can go out baking room；
Step S4, semi-finished product attrition grinding is to powder：By the semi-finished product after drying, put into pulverizer and be ground,
Until becoming powder；
Step S5, semi-finished product bacteria containing amount detects：By the semi-finished product powder after grinding, the quality inspection of quality inspection portion is sent, detects bacteria containing amount
Whether standard is met；
Step S6, finished product packing is put in storage：Qualified finished product is packed, mounted box, vanning, be stored in shady and cool, ventilation, dry
Storehouse in.
Further, when fermentation time is 24 small in the step S1, fermenting house temperature maintains 30~40 DEG C.
Further, it is 3~5% that semi-finished product moisture is up to standard in the step S3, preferably, being checked per half an hour
Semi-finished product moisture, until up to standard.
Further, in the step S3 baking room temperature control at 45~50 DEG C.
Further, in the step S4 powder particle diameter≤0.4mm.
Further, bacteria containing amount criterion of acceptability is effective bacterial content >=1.0 × 10 of every gram of product in the step S58。
Compared with prior art, the present invention with following technique effect：
(1) complex microorganism preparations of the invention are widely used, can be used for the growth for suppressing pathogenic bacteria, degraded moss and
Filamentous algae, removes cyanobacteria, stabilizing water quality and algae phase；
(2) complex microorganism preparations of the invention can be used for ammonia nitrogen in degradation water, nitrite, decompose the excrement in pond
Just organic matter being waited, the probiotics contained by it can form biological protection film in aquatic livestock body surface, it is ensured that aquatic health is grown,
Aquatic health can be significantly improved by feeding fermented feed, prevent introduced disease；
(3) complex microorganism preparations of the present invention are formed by natural material fermentation, no added antibiotic etc, to water
Body is pollution-free.
Embodiment
Below in conjunction with specific embodiment, carry out the technical solution that the present invention will be described in detail.Raw material in embodiment is city
Product is sold, wherein, Guangdong Province microorganism can be selected in bacillus subtilis bacterium powder, lactobacillus acidophilus bacterium powder, bifidobacterium longum bacterium powder
The product that research institute provides.
Embodiment one
A kind of complex microorganism preparations, it includes following components in percentage by weight：Fermentation material 50%, fish meal 10%, rice
Chaff 20%, soybean protein 5%, starch 15%；Fermentation material includes following components in percentage by weight：Calcium carbonate 35%, withered grass bud
Spore bacillus bacterium powder 1%, probiotics bacterium powder 1%, tapioca starch 5%, soy meal 10%, carbohydrate 3%, yeast 2.4%, composite mineral salt
0.6%th, water 42%；Probiotics bacterium powder includes following components in percentage by weight：Lactobacillus acidophilus bacterium powder 30%, bifidobacterium longum
Bacterium powder 30%, Lactobacillus casei bacterium powder 40%.
Complex microorganism preparations in the present embodiment, are made by following steps：
The preparation of step 1, fermentation material：Fermentation material component is stirred mixing according to the amount of percentage by weight, mixing is equal
After even, put to fermentation cask fermentation 24 it is small when, be positioned over during fermentation in fermenting house, fermenting house temperature maintains 30~40 DEG C；
The preparation of step 2, mixed fermentation material：By the fermentation material fermented according to fermentation material 50%, fish meal 10%, rice bran
20%th, soybean protein 5%, the amount of percentage by weight of starch 10% are sufficiently mixed；
The preparation of step 3, semi-finished product：Mixed fermentation material is dispensed by 2 kilograms/disk, subsequently into baking room, half into
Product moisture goes out baking room after reaching 3~5%.During drying, baking room temperature control is checked at 45~50 DEG C, per half an hour
Semi-finished product moisture, until up to standard；
Step 4, semi-finished product attrition grinding to powder：By the semi-finished product after drying, put into pulverizer and be ground, powder
Last particle diameter≤0.4mm, aperture is controlled with punching net；
Step 5, the detection of semi-finished product bacteria containing amount：Semi-finished product after grinding, send quality inspection portion to carry out quality inspection, and criterion of acceptability is：Often
Gram effective bacterial content >=1.0 × 10 of product8；
Step 6, finished product packing storage：Pouch-packaged is carried out by 100g/ bags, then it is box-packed by 1kg (10 bag)/box progress, most
Joint sealing packaging is carried out by 12 boxes/case afterwards, is stored in shady and cool, ventilation, dry storehouse.
Embodiment two
A kind of complex microorganism preparations, it includes following components in percentage by weight：Fermentation material 45%, fish meal 12%, rice
Chaff 25%, soybean protein 4%, starch 14%；Fermentation material includes following components in percentage by weight：Calcium carbonate 35%, withered grass bud
Spore bacillus bacterium powder 1%, probiotics bacterium powder 1%, tapioca starch 5%, soy meal 10%, carbohydrate 3%, yeast 2.4%, composite mineral salt
0.6%th, water 42%；Probiotics bacterium powder includes following components in percentage by weight：Lactobacillus acidophilus bacterium powder 30%, bifidobacterium longum
Bacterium powder 30%, Lactobacillus casei bacterium powder 40%.
It is identical with embodiment one that complex microorganism preparations step in the present embodiment is made.
Embodiment three
A kind of complex microorganism preparations, it includes following components in percentage by weight：Fermentation material 55%, fish meal 8%, rice bran
15%th, soybean protein 6%, starch 16%；Fermentation material includes following components in percentage by weight：Calcium carbonate 35%, withered grass gemma
Bacillus bacterium powder 1%, probiotics bacterium powder 1%, tapioca starch 5%, soy meal 10%, carbohydrate 3%, yeast 2.4%, composite mineral salt
0.6%th, water 42%；Probiotics bacterium powder includes following components in percentage by weight：Lactobacillus acidophilus bacterium powder 30%, bifidobacterium longum
Bacterium powder 30%, Lactobacillus casei bacterium powder 40%.
The step of complex microorganism preparations in the present embodiment are made is identical with embodiment one.
Experiment one
Experiment selection is cultivated using outdoor earth pond, sets 2 test tanks, 1 control pond.This 3 ponds are in area, water
Depth, aquaculture model, stocking rate, corollary equipment, feeding and management condition etc. all same.Pond area is 2 mu, the depth of water
1.5m, 2.2kW impeller automatic aerators 1 are matched somebody with somebody per pond, and the thick seedling of Penaeus Vannmei mark is launched in pond, and prawn initially averagely grow by body
1.51cm。
First carry out temporarily supporting 7 days, then start to launch the complex microorganism preparations of embodiment one, the use of microbial agent
Situation refers to table 1：
1 test tank condition of table
| Test No. 1 pond | Test No. 2 ponds | Test No. 3 ponds | |
| Area (mu) | 2 | 2 | 2 |
| The depth of water (rice) | 1.5 | 1.5 | 1.5 |
| Preparation dosage (g/ mus) | 1000 | 1000 | 0 |
Test period 10 days by a definite date.Complex microorganism preparations are applied 1 time daily.This experiment mainly monitoring water body in ammonia nitrogen,
The change feelings of the items such as quantity of vibrio parahaemolytious, vibrio alginolyticus water quality index in nitrite, chemical oxygen consumption (COC) (COD), water
Condition, ammonia nitrogen, nitrite, the reference of chemical oxygen consumption (COC) (COD) water quality index assay method in water body《Marine monitoring specification》(BG
1737814-1998).Vibrio parahaemolytious, the quantitative index of vibrio alginolyticus go out each experiment using TCBS Screening of Media meters in water
Vibrio parahaemolytious, the quantity of vibrio alginolyticus in group water body.
The detection sampling time is set to the morning 9 daily：00, by special messenger underwater 50cm water layers water sample, physics and chemistry are gathered with hydrophore
In experimental determination after factored sampling.
By the measuring of 10 days, the variation effect of ammonia nitrogen was shown in Table 2 in each group water.
The change of ammonia nitrogen in 2 each group water of table
From table 2 it can be seen that No. 1 pond and No. 2 pond ammonia nitrogens decrease by 100%, No. 3 pond ammonia nitrogen concentrations of control group but persistently increase
Add, illustrate that complex microorganism preparations can effectively degrade ammonia nitrogen concentration in the leg Shrimp Litopenaeus vannamei Culture Raceway-type Ponds pool.
By the measuring of 10 days, the variation effect of each group water nitrite was shown in Table 3.
The change of 3 each group water nitrite of table
From table 3 it can be seen that No. 1 pond and No. 2 pond nitrite decrease by 100%, No. 3 pond nitrite concentrations of control group
But continue to increase, illustrate that complex microorganism preparations can effectively degrade leg Shrimp Litopenaeus vannamei Culture Raceway-type Ponds pool nitrite concentration.
By the measuring of 10 days, the variation effect of chemical oxygen consumption (COC) (COD) was shown in Table 4 in each group water.
Chemical oxygen consumption (COC) (COD in 4 each group water of tablecr)) change (420nm)
From table 4, it can be seen that No. 1 pond and No. 2 pond chemical oxygen consumption (COC) (COD) concentration decrease by 63%, No. 3 ponds of control group
Learn oxygen demand (COD) concentration but to continue to increase, illustrate that complex microorganism preparations can effectively degrade in the leg Shrimp Litopenaeus vannamei Culture Raceway-type Ponds pool
Chemical oxygen consumption (COC) (COD) concentration.
By the measuring of 10 days, the variation effect of vibrios was shown in Table 5 in each group water.
The change of vibrios in 5 each group water of table
As can be seen from Table 5, amount of vibrio has decline in No. 1 pond and No. 2 waters, vibrios number in No. 3 waters of control group
Amount but increased, and illustrate that complex microorganism preparations can suppress amount of vibrio in the water of the degraded leg Shrimp Litopenaeus vannamei Culture Raceway-type Ponds pool.
Experiment two
Experiment selection is cultivated using indoor cement pit, sets 2 test tanks, 1 control pond.This 3 cement pits area,
The depth of water, aquaculture model, stocking rate, corollary equipment, feeding and management condition etc. all same.Cement pit is 16m2Area, pond
The pool launch the thick seedling of Penaeus Vannmei mark, prawn initially be averaged the long 1.65cm of body.
First carry out temporarily supporting 7 days, then start to launch the complex microorganism preparations of embodiment one, the use of microbial agent
Situation refers to table 6：
6 test tank condition of table
| Test No. 1 pond | Test No. 2 ponds | Test No. 3 ponds | |
| Area (m2) | 16 | 16 | 16 |
| The depth of water (rice) | 1.2 | 1.2 | 1.2 |
| Preparation dosage (g) | 50 | 50 | 0 |
Test period 10 days by a definite date.Complex microorganism preparations are applied 1 time daily.This experiment mainly monitoring water body in ammonia nitrogen,
The situation of change of the items water quality index such as nitrite, chemical oxygen consumption (COC) (COD).It is measured immediately after water sample collection in worksite.
Assay method refers to《Marine monitoring specification》(BG 1737814-1998).Because cement pit uses industrial circulating water cultivating, water body
Middle amount of vibrio is few, therefore amount of vibrio does not measure in water body.
The detection sampling time is set to the morning 9 daily：00, by special messenger underwater 50cm water layers water sample, physics and chemistry are gathered with hydrophore
In experimental determination after factored sampling.
By the measuring of 10 days, the variation effect of ammonia nitrogen was shown in Table 7 in each group water.
The change of ammonia nitrogen in 7 each group water of table
As can be seen from Table 7, No. 1 pond and No. 2 pond ammonia nitrogen concentration increases are less, and less than 3 times, No. 3 pond ammonia nitrogens of control group are dense
Degree increase is very fast, reaches 8 times or so, illustrates that the complex microorganism preparations ammonia nitrogen in the leg Shrimp Litopenaeus vannamei Culture Raceway-type Ponds pool that can effectively degrade is dense
Degree.
By the measuring of 10 days, the variation effect of each group water nitrite was shown in Table 8.
The change of 8 each group water nitrite of table
As can be seen from Table 8, No. 1 pond and No. 2 pond nitrite first increase and then reduce, No. 3 pond nitrite of control group
Concentration but continues to increase, finally more than 3 times bigger than test group, illustrates that the complex microorganism preparations Penaeus Vannmei that can effectively degrade is supported
Grow pond nitrite concentration.
By the measuring of 10 days, the variation effect of chemical oxygen consumption (COC) (COD) was shown in Table 9 in each group water.
Chemical oxygen consumption (COC) (COD in 9 each group water of tablecr)) change (420nm)
As can be seen from Table 9, No. 1 pond and No. 2 pond chemical oxygen consumption (COC) (COD) concentration continue to decline, No. 3 pond chemistry of control group
Oxygen demand (COD) concentration but continues to increase, and illustrates complex microorganism preparations effectively to degrade in the leg Shrimp Litopenaeus vannamei Culture Raceway-type Ponds pool and changes
Learn oxygen demand (COD) concentration.
Had by the visible complex microorganism preparations of the invention of upper experiment to ammonia nitrogen, nitrite in degradation water, and decomposition
Machine thing has significant effect, and can suppress germ, such as vibrios, increases the dissolved oxygen content in water body, improves Water quality.
Above-described embodiment be only the present invention section Example, but can not as the limitation to technical solution of the present invention,
Any improvement and modification based on the basis of present inventive concept, should all fall within the scope of protection of the invention, specifically protect
Scope is subject to what claims were recorded.
Claims (10)
1. a kind of complex microorganism preparations, it is characterised in that include following components in percentage by weight：Fermentation material 45-55%, fish
Powder 8-12%, rice bran 15-25%, soybean protein 4-6%, starch 12-18%.
2. complex microorganism preparations according to claim 1, it is characterised in that the fermentation material includes following weight percent
The component of ratio：Calcium carbonate 35%, bacillus subtilis bacterium powder 1%, probiotics bacterium powder 1%, tapioca starch 5%, soy meal 10%, sugar
Class 3%, yeast 2.4%, composite mineral salt 0.6%, water 42%.
3. complex microorganism preparations according to claim 1 or 2, it is characterised in that the probiotics bacterium powder includes following
Component：Lactobacillus acidophilus bacterium powder, bifidobacterium longum bacterium powder, Lactobacillus casei bacterium powder.
4. complex microorganism preparations according to claim 3, it is characterised in that the lactobacillus acidophilus bacterium powder, long bifid
Bacillus bacterium powder, Lactobacillus casei bacterium powder percentage by weight are 30%：30%：40%.
5. the preparation method of claim 1-4 any one of them complex microorganism preparations, it is characterised in that including following step
Suddenly：
Step S1, the preparation of fermentation material：Fermentation material component is stirred mixing according to the amount of percentage by weight, is uniformly mixed
Afterwards, put to fermentation cask fermentation, fermenter is placed in fermenting house during fermentation；
Step S2, the preparation of mixed fermentation material：By the fermentation material fermented, fish meal, rice bran, soybean protein and starch by weight hundred
The amount of ratio is divided to be sufficiently mixed；
Step S3, the preparation of semi-finished product：Mixed fermentation material is dispensed, subsequently into baking room, semi-finished product moisture reaches
Mark can go out baking room；
Step S4, semi-finished product attrition grinding is to powder：By the semi-finished product after drying, put into pulverizer and be ground, until
Become powder；
Step S5, semi-finished product bacteria containing amount detects：By the semi-finished product powder after grinding, the quality inspection of quality inspection portion is sent, whether is detection bacteria containing amount
Meet standard；
Step S6, finished product packing is put in storage：Qualified finished product is packed, mounted box, vanning, be stored in shady and cool, ventilation, dry storehouse
Fang Li.
6. the preparation method of complex microorganism preparations according to claim 5, it is characterised in that ferment in the step S1
When time is 24 small, fermenting house temperature maintains 30~40 DEG C.
7. the preparation method of complex microorganism preparations according to claim 6, it is characterised in that in the step S3 half into
Product moisture up to standard is 3~5%.
8. the preparation method of the complex microorganism preparations according to claim 6 or 7, it is characterised in that in the step S3
Baking room temperature control is at 45~50 DEG C.
9. the preparation method of complex microorganism preparations according to claim 8, it is characterised in that powder in the step S4
Particle diameter≤0.4mm.
10. according to the preparation method of 5,6,7,9 any one of them complex microorganism preparations of claim, it is characterised in that institute
It is effective bacterial content >=1.0 × 10 of every gram of product to state bacteria containing amount criterion of acceptability in step S58。
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020151157A1 (en) * | 2019-01-24 | 2020-07-30 | 东南大学 | Biological agent for degrading algae, preparation method therefor and use thereof |
| CN114084964A (en) * | 2020-08-24 | 2022-02-25 | 中国石油化工股份有限公司 | Aromatic organic compound wastewater biological treatment accelerant and preparation method and application thereof |
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| CN114084964A (en) * | 2020-08-24 | 2022-02-25 | 中国石油化工股份有限公司 | Aromatic organic compound wastewater biological treatment accelerant and preparation method and application thereof |
| CN114084964B (en) * | 2020-08-24 | 2023-02-24 | 中国石油化工股份有限公司 | Aromatic organic compound wastewater biological treatment accelerant and preparation method and application thereof |
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