CN107982340A - A kind of refined substance of Cortex Phellodendri and its preparation method and application - Google Patents
A kind of refined substance of Cortex Phellodendri and its preparation method and application Download PDFInfo
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- CN107982340A CN107982340A CN201711387959.5A CN201711387959A CN107982340A CN 107982340 A CN107982340 A CN 107982340A CN 201711387959 A CN201711387959 A CN 201711387959A CN 107982340 A CN107982340 A CN 107982340A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
- A61K36/756—Phellodendron, e.g. corktree
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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Abstract
The present invention relates to the technical field of Chinese medical extract, more particularly to a kind of purification of tcm thing and preparation method and application.The refined substance carries through water using Cortex Phellodendri as raw material, UF membrane, ion-exchange chromatography, is concentrated and dried to obtain Cortex Phellodendri refined substance power-product.Cortex Phellodendri refined substance dry powder uniform color prepared by the present invention, solubility is good, purity is high, active constituent content is high and the effect of adjuvant therapy rheumatoid arthritis is preferable；Have the characteristics that preparation process is easy and effective in production process, be not required poisonous and hazardous organic solvent, cost low and discharged without " three wastes ".The product prepared using the method for the present invention can be widely applied in the industries such as medicine, health care, and have boundless market prospects, can produce huge economic benefit and good social benefit.
Description
Technical field
The present invention relates to a kind of preparation technique of traditional Chinese medicine, more particularly to a kind of extraction technology of preparing of effective component of chinese medicine and its
Using, and in particular to it is a kind of using UF membrane, ion-exchange chromatography and the method for being concentrated and dried technology preparation Cortex Phellodendri refined substance.
Background technology
Rheumatoid arthritis is also known as rheumatoid, is a kind of autoimmune disease, its pathogenic factor may with endocrine,
Nutrition, geography, occupation, the difference of psychology and social environment, bacterium and virus infection and inherent cause etc. have relation, with slow
Property, symmetry, more synovial joints be scorching and joint outside lesion be main clinical manifestation, belong to autoimmune inflammatory disease.
The many persons of rheumatoid arthritis are " not dead cancer ", there is no specific short, common antirheumatic drug at present
And hormone preparation curative effect owes preferable, first, it is slow to work, just mitigate pain after a few hours, unobvious of subsiding a swelling；Second, prolonged application
There is side effect to sclerotin, alimentary canal, angiocarpy, bleeding etc., must be careful anti-.The theory of the traditional Chinese medical science is different from doctor trained in Western medicine, it is believed that quasi-wind gateway is
Caused by the wet product of qi deficiency to blood stasis, suitable strengthening vital QI to eliminate pathogenic factors is controlled.The drug effect therapy target of Chinese traditional treatment method is clear and definite, and toxic side effect is less,
Patient to be limited using medicine for oral administration provides new therapeutic choice.
Chinese medicine has long medicinal history, is the treasure of Chinese traditional culture.Chinese medicine is a complex system, its
Clinical efficacy explanation wherein includes mass efficient material, while there is also some in Chinese medicine to bring the poisonous of security risk to have
Evil material.Therefore it is in one in traditional Chinese medicine research field important to extract its active ingredient to develop new and effective preparation technique of traditional Chinese medicine
Hold.Traditional Chinese medicine extraction is exactly to extract wherein effective ingredient to greatest extent using some new techniques so that the inherent matter of Chinese medicine preparation
Amount and clinical therapeutic efficacy improve, and the effect of Chinese medicine is played to greatest extent.Membrane separation technique has different points of separation
The function of son amount, the technology have with traditional separation method compared with separation process without phase-change, separative efficiency it is high, need not addition it is chemical
Reagent, mild condition, without component damage, flow is short the advantages that, with applied to separating effective Chinese medicine components.
Cortex Phellodendri is conventional Chinese medicine, its cold in nature, bitter, tool purging intense heat and detonicating, heat-clearing and damp-drying drug and other effects.Now research shows, Cortex Phellodendri
Containing principle active components such as jamaicin, obakunones, there are antibacterial, convergence, anti-inflammatory.Research shows that Cortex Phellodendri is as a kind of weight
The traditional Chinese medicine ingredients wanted, there is significant effect in treating rheumatoid arthritis.Therefore, the new preparation of Cortex Phellodendri refined substance is developed
Technique has great importance
The content of the invention
Present invention generally provides one kind using UF membrane, ion-exchange chromatography and to be concentrated and dried technology preparation Cortex Phellodendri refined substance
Method, asked for solving in current Cortex Phellodendri extraction process the technologies such as yield is low, process costs are high, preparation process stability difference
Topic.
To overcome the problems of the prior art, a kind of preparation method of Cortex Phellodendri refined substance proposed by the present invention, including it is as follows
Step：
Water extracts：Cortex Phellodendri medicinal material is taken, adds water to carry out decoction 0.5-24h, leaching liquid, the dregs of a decoction, which add water, decocts about 0.5-
12h, leaching liquid, discards the dregs of a decoction；Merge liquid twice, up to aqueous extract；
Crude separation：Decoction liquor is subjected to UF membrane, used filter membrane specification is 0.22-0.65 μm or 500-1000kD,
Filter wash is carried out with purified water after having filtered, merges film permeate, obtains rough segmentation chaotropic；
Segment from：Rough segmentation chaotropic is subjected to ultrafiltration membrane classification separation, used ultrafiltration membrane specification is 1-100kD, filtering
Filter wash is carried out with purified water after complete, merges film permeate, obtains subdivision chaotropic；
It is refined：Subfractionation liquid is subjected to purifying decoloration by ion exchange column, column is collected and flows through liquid, obtain refined liquid,
It is concentrated and dried, up to Cortex Phellodendri refined substance powder.
Wherein, it is purified water, distilled water or water for injection with water in water extraction step.
Wherein, use can be that hollow fiber form, rolled film, tubular membrane or plate and frame film carry out UF membrane.
Wherein, crude separation and segment from, filter wash number is 3-5 times.
Wherein, the structural formula of the aglucon of the ion-exchange chromatography resin for following molecular structural formula (i)~(iii) it
One, loading volume is 10-50 times of column volume：
Wherein, in purification step, concentrate drying technology used is freeze-drying or spray drying.
The present invention also proposes a kind of Cortex Phellodendri refined substance according to prepared by the preparation method, and the turbidity of Cortex Phellodendri refined substance is shallow
In or equal to 0.5 level number of turbidity standard turbidity standard, freeze-dried powder is highly soluble in water and solubility is not less than 0.5g/ml.
The present invention also proposes application of the Cortex Phellodendri refined substance in the medicine of adjuvant therapy rheumatoid arthritis is prepared.
In above application, the medicine is preparation, includes Cortex Phellodendri refined substance or Cortex Phellodendri refined substance and auxiliary material.
Compared with prior art, beneficial effects of the present invention：The present invention provides a kind of stable quality, and curative effect is reliable, biology
The arthritic Cortex Phellodendri Chinese medicine preparation of the high auxiliary curing rheumatoid disease of availability.It is arthritic that the present invention provides auxiliary curing rheumatoid disease
The preparation method of Cortex Phellodendri Chinese medicine preparation, this method is simple and feasible, meets oral drugs body absorption process, final obtained Chinese medicine
Better stability of preparation, curative effect are reliably notable.The present invention prepares Cortex Phellodendri refined substance using UF membrane and ion-exchange chromatography can
The purity of object and the content of active ingredient are significantly improved, this method has saved medicinal material cost, using the teaching of the invention it is possible to provide is more advantageous to work
Industry produces preparation condition, and ensures the stability and security of technique, Cortex Phellodendri Chinese medicine is played its effect as far as possible.The present invention
The characteristics of be：The method being combined using micro-filtration, ultrafiltration and chromatographic technique, it is residual can effectively to remove lipid, pigment, heavy metal, agriculture
Etc. invalid components, while avoid the use of organic reagent, reduce industrial production pollution；Active ingredient is more importantly remained,
The medical value for improving Cortex Phellodendri medicinal material can be maximized
Brief description of the drawings
Fig. 1 is Cortex Phellodendri Aqueous extracts freeze-dried powder and Cortex Phellodendri refined liquid freeze-dried powder and its schematic diagram of redissolution liquid.
Fig. 2 is Aqueous extracts and refined liquid HPLC analysis results.
Embodiment
Cortex Phellodendri refined substance proposed by the present invention and its preparation method and application is carried out below in conjunction with schematic diagram more detailed
Description, which show the preferred embodiment of the present invention, it should be appreciated that those skilled in the art can change described here
The present invention, and still realize the advantageous effects of the present invention.Therefore, description below is appreciated that for those skilled in the art
It is widely known, and be not intended as limitation of the present invention.
The creation point of the present invention is UF membrane, ion-exchange chromatography and concentrate drying technology being applied to carrying for Chinese medicine
In terms of taking purifying, significant technological progress is thus brought, solution Chinese medical extract poor appearance, color are deep and preserve easily precipitation etc.
Shortcoming.With reference to extractive technique, UF membrane filtering technique, chromatographic technique and concentration technique etc., development is set of, can industrialization height
Separation and preparation technology is imitated, that establishes different Chinese medicine effective efficiency components efficiently separates preparation process, and is removed in separation process
The poisonous and harmful substances such as pesticide residue, heavy metal, endotoxin, carry out the process exploitation from lab scale to pilot scale to industrialization, verify
With conversion, form the safe and efficient application for isolating and purifying the high-end preparation of Chinese medicine such as technology of preparing application, development characteristic formulations and push away
Wide research, the overall international competitiveness for improving Chinese medicine preparation.
Embodiment 1
Cortex Phellodendri 100g is weighed, adds 8 times of water 0.8L to be put into boiling machine and boils (95 DEG C) 1.5h, filtered through gauze, the dregs of a decoction add 4 times of water
0.4L, which is placed again into boiling machine, boils (95 DEG C) 1h, filtered through gauze, merging filtrate, UF membrane filtering clarification and purifying.
0.45 μm of microfiltration membranes is connected on film processing system, flow velocity 400rpm is set, circulates end flow velocity 1.5L/min, thoroughly
It is 18ml/min to cross end mean flow rate, collects permeate, and end liquid volume to be recycled is down to 80-100ml, with purified water filter wash,
Each 50ml, filter wash 5 times；Merge film permeate, obtain Cortex Phellodendri water and put forward rough segmentation chaotropic.
100kD ultrafiltration membranes are connected on film processing system, flow velocity 360rpm is set, circulate end flow velocity 1.2L/min, thoroughly
It is 15ml/min to cross end mean flow rate, collects permeate, and end liquid volume to be recycled is down to 80-100ml, with purified water filter wash,
Each 50ml, filter wash 5 times；Merge permeate, obtain Cortex Phellodendri water and carry subfractionation liquid.
By Q Sepharose 6FF, (molecular structural formula of resin aglucon is) ion exchange column company
It is connected in tomographic system, design current velocity 10ml/min (250cm/h), Cortex Phellodendri water carries subfractionation liquid and crosses Q columns by systems pumps, collects
Column flows through liquid, obtains refined liquid；Refined liquid is positioned over -40 DEG C of refrigerator freezing 5h, after be put into freeze dryer, vacuumize it is dry until
Moisture all removes, and collects freeze-dried powder, is Cortex Phellodendri refined substance powder product.
Cortex Phellodendri water extract and refined substance are taken respectively, pass through the change of target peak shape after HPLC analyses before purification, HPLC conditions
It is as follows：(1) chromatographic column filler：Unitary C18 (5 μm, 4.6*250mm)；(2) mobile phase A：0.4% (V/V) phosphoric acid, flowing
Phase B：Acetonitrile；(3) flow velocity：1ml/min；(4) gradient condition：0-10min 5%B phases, 10-30min is from 5-30%B phases, 30-
60min is from 30-95%B phases, 60-70min 95%B phases.(5) column temperature：50℃；(6) Detection wavelength：205nm；(7) sample introduction body
Product：10μl.
From the appearance, Cortex Phellodendri refined substance powder uniform color, any surface finish, quality are fine and smooth.Refined substance freeze-dried powder finished product
Dissolved rapidly after adding purified water, solution clarification is bright, and the dissolving of water extract freeze-dried powder is slow and muddy, the result is shown in Figure 1.HPLC points
The results show (as shown in Figure 2) is analysed, rear sample, which obtains main peak shape in collection of illustrative plates, before purification does not have significant change, according to both cumulative volumes
Calculated with integrated peak areas, Cortex Phellodendri refined substance main component yield does not decline after purification.Result above fully indicates use
The method of the present invention prepares the advantage with high-purity in high yield of Cortex Phellodendri refined substance.
Embodiment 2
Cortex Phellodendri 100g is weighed, adds 10 times of water 1L to be put into boiling machine and boils (95 DEG C) 1.5h, filtered through gauze, the dregs of a decoction add 6 times of water
0.6L, which is placed again into boiling machine, boils (95 DEG C) 1h, filtered through gauze, merging filtrate, UF membrane filtering clarification and purifying.
0.22 μm of microfiltration membranes is connected on film processing system, flow velocity 400rpm is set, circulates end flow velocity 1.5L/min, thoroughly
It is 16ml/min to cross end mean flow rate, collects permeate, and end liquid volume to be recycled is down to 80-100ml, with purified water filter wash,
Each 50ml, filter wash 5 times；Merge film permeate, obtain Cortex Phellodendri water and put forward rough segmentation chaotropic.
50kD ultrafiltration membranes are connected on film processing system, flow velocity 360rpm is set, circulate end flow velocity 1.2L/min, are passed through
End mean flow rate is 15ml/min, collects permeate, and end liquid volume to be recycled is down to 80-100ml, with purified water filter wash, often
Secondary 50ml, filter wash 5 times；Merge permeate, obtain Cortex Phellodendri water and carry subfractionation liquid.
By DEAE Sepharose 6FF, (molecular structural formula of resin aglucon is) ion exchange column
It is connected in tomographic system, design current velocity 10ml/min (250cm/h), Cortex Phellodendri water carries subfractionation liquid and crosses Q columns by systems pumps, receives
Clustered column flows through liquid, obtains refined liquid；Refined liquid is positioned over -40 DEG C of refrigerator freezing 5h, after be put into freeze dryer, vacuumize dry straight
All removed to moisture, collect freeze-dried powder, be Cortex Phellodendri refined substance powder product.
Embodiment 3
Cortex Phellodendri 100g is weighed, adds 8 times of water 0.8L to be put into boiling machine and boils (95 DEG C) 1.5h, filtered through gauze, the dregs of a decoction add 5 times of water
0.5L, which is placed again into boiling machine, boils (95 DEG C) 1h, filtered through gauze, merging filtrate, UF membrane filtering clarification and purifying.
0.1 μm of microfiltration membranes is connected on film processing system, flow velocity 380rpm is set, circulates end flow velocity 1.4L/min, thoroughly
It is 15ml/min to cross end mean flow rate, collects permeate, and end liquid volume to be recycled is down to 80-100ml, with purified water filter wash,
Each 50ml, filter wash 5 times；Merge film permeate, obtain Cortex Phellodendri water and put forward rough segmentation chaotropic.
30kD ultrafiltration membranes are connected on film processing system, flow velocity 350rpm is set, circulate end flow velocity 1.2L/min, are passed through
End mean flow rate is 13ml/min, collects permeate, and end liquid volume to be recycled is down to 80-100ml, with purified water filter wash, often
Secondary 50ml, filter wash 5 times；Merge permeate, obtain Cortex Phellodendri water and carry subfractionation liquid.
By WorkBeads 100TREN, (molecular structural formula of resin aglucon is) ion exchange
Chromatographic column is connected in tomographic system, and design current velocity 10ml/min (250cm/h), Cortex Phellodendri water carries subfractionation liquid and passes through systems pumps mistake
Q columns, collect column and flow through liquid, obtain refined liquid；Refined liquid is positioned over -40 DEG C of refrigerator freezing 5h, after be put into freeze dryer, vacuumize
Drying collects freeze-dried powder, as Cortex Phellodendri refined substance powder product until moisture all removals.
Embodiment 4
Cortex Phellodendri 100g is weighed, adds 12 times of water 1.2L to be put into boiling machine and boils (95 DEG C) 1.5h, filtered through gauze, the dregs of a decoction add 6 times
Water 0.6L, which is placed again into boiling machine, boils (95 DEG C) 1h, filtered through gauze, merging filtrate, UF membrane filtering clarification and purifying.
750kD ultrafiltration membranes are connected on film processing system, flow velocity 380rpm is set, circulate end flow velocity 1.3L/min, thoroughly
It is 15ml/min to cross end mean flow rate, collects permeate, and end liquid volume to be recycled is down to 80-100ml, with purified water filter wash,
Each 50ml, filter wash 5 times；Merge film permeate, obtain Cortex Phellodendri water and put forward rough segmentation chaotropic.
10kD ultrafiltration membranes are connected on film processing system, flow velocity 330rpm is set, circulate end flow velocity 1.1L/min, are passed through
End mean flow rate is 11ml/min, collects permeate, and end liquid volume to be recycled is down to 80-100ml, with purified water filter wash, often
Secondary 50ml, filter wash 5 times；Merge permeate, obtain Cortex Phellodendri water and carry subfractionation liquid.
By Q Sepharose 6FF, (molecular structural formula of resin aglucon is) ion exchange column company
It is connected in tomographic system, design current velocity 10ml/min (250cm/h), Cortex Phellodendri water carries subfractionation liquid and crosses Q columns by systems pumps, collects
Column flows through liquid, obtains refined liquid；Refined liquid is crossed into spray dryer until moisture all removes, collection dried powder, is Cortex Phellodendri
Refined substance powder product.
Embodiment 5
Cortex Phellodendri 100g is weighed, adds 15 times of water 1.5L to be put into boiling machine and boils (95 DEG C) 1.5h, filtered through gauze, the dregs of a decoction add 6 times
Water 0.6L, which is placed again into boiling machine, boils (95 DEG C) 1h, filtered through gauze, merging filtrate, UF membrane filtering clarification and purifying.
750kD ultrafiltration membranes are connected on film processing system, flow velocity 380rpm is set, circulate end flow velocity 1.3L/min, thoroughly
It is 15ml/min to cross end mean flow rate, collects permeate, and end liquid volume to be recycled is down to 80-100ml, with purified water filter wash,
Each 50ml, filter wash 5 times；Merge film permeate, obtain Cortex Phellodendri water and put forward rough segmentation chaotropic.
100kD ultrafiltration membranes are connected on film processing system, flow velocity 360rpm is set, circulate end flow velocity 1.2L/min, thoroughly
It is 15ml/min to cross end mean flow rate, collects permeate, and end liquid volume to be recycled is down to 80-100ml, with purified water filter wash,
Each 50ml, filter wash 5 times；Merge permeate, obtain Cortex Phellodendri water and carry subfractionation liquid.
By Q Sepharose 6FF, (molecular structural formula of resin aglucon is) ion exchange column company
It is connected in tomographic system, design current velocity 10ml/min (250cm/h), Cortex Phellodendri water carries subfractionation liquid and crosses Q columns by systems pumps, collects
Column flows through liquid, obtains refined liquid；Refined liquid is crossed into spray dryer until moisture all removes, collection dried powder, is Cortex Phellodendri
Refined substance powder product.
The preferred embodiment of the present invention is above are only, does not play the role of any restrictions to the present invention.Belonging to any
Those skilled in the art, in the range of technical scheme is not departed from, to the invention discloses technical solution and
Technology contents make the variation such as any type of equivalent substitution or modification, belong to the content without departing from technical scheme, still
Belong within protection scope of the present invention.
Claims (9)
1. a kind of preparation method of Cortex Phellodendri refined substance, it is characterised in that include the following steps：
Water extracts：Cortex Phellodendri medicinal material is taken, adds water to carry out decoction 0.5-24h, leaching liquid, the dregs of a decoction, which add water, decocts about 0.5-12h, filter
Taking liquid, discards the dregs of a decoction；Merge liquid twice, up to aqueous extract；
Crude separation：Decoction liquor is subjected to UF membrane, used filter membrane specification is 0.22-0.65 μm or 500-1000kD, filtering
Filter wash is carried out with purified water after complete, merges film permeate, obtains rough segmentation chaotropic；
Segment from：Rough segmentation chaotropic is subjected to ultrafiltration membrane classification separation, used ultrafiltration membrane specification is 1-100kD, after having filtered
Filter wash is carried out with purified water, merges film permeate, obtains subdivision chaotropic；
It is refined：Subfractionation liquid is subjected to purifying decoloration by ion exchange column, column is collected and flows through liquid, refined liquid is obtained, by it
It is concentrated and dried, up to Cortex Phellodendri refined substance powder.
2. preparation method according to claim 1, it is characterised in that be purified water, distilled water with water in water extraction step
Or water for injection.
3. preparation method according to claim 1, it is characterised in that use can be hollow fiber form, rolled film, tubular type
Film or plate and frame film carry out UF membrane.
4. preparation method according to claim 1, it is characterised in that crude separation and segment from, filter wash number is 3-5
It is secondary.
5. preparation method according to claim 1, it is characterised in that the structure of the aglucon of the ion-exchange chromatography resin
Formula is one of following molecular structural formula (i)~(iii), and loading volume is 10-50 times of column volume：
6. preparation method according to claim 1, it is characterised in that in purification step, concentrate drying technology used is cold
Lyophilized dry or spray drying.
A kind of 7. Cortex Phellodendri refined substance prepared by any one of them preparation method according to claim 1-6, it is characterised in that
The turbidity of Cortex Phellodendri refined substance is shallower than or the turbidity standard equal to 0.5 level number of turbidity standard, and freeze-dried powder is highly soluble in water and molten
Xie Du is not less than 0.5g/ml.
8. application of the Cortex Phellodendri refined substance in the medicine of adjuvant therapy rheumatoid arthritis is prepared described in claim 7.
9. application according to claim 8, it is characterised in that the medicine is preparation, includes Cortex Phellodendri refined substance or Cortex Phellodendri
Refined substance and auxiliary material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711387959.5A CN107982340A (en) | 2017-12-20 | 2017-12-20 | A kind of refined substance of Cortex Phellodendri and its preparation method and application |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711387959.5A CN107982340A (en) | 2017-12-20 | 2017-12-20 | A kind of refined substance of Cortex Phellodendri and its preparation method and application |
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| CN107982340A true CN107982340A (en) | 2018-05-04 |
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| CN201711387959.5A Pending CN107982340A (en) | 2017-12-20 | 2017-12-20 | A kind of refined substance of Cortex Phellodendri and its preparation method and application |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101530495A (en) * | 2008-01-22 | 2009-09-16 | 上海中药制药技术有限公司 | Method for preparing phellodendron extract by applying membrane filtration technology |
-
2017
- 2017-12-20 CN CN201711387959.5A patent/CN107982340A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101530495A (en) * | 2008-01-22 | 2009-09-16 | 上海中药制药技术有限公司 | Method for preparing phellodendron extract by applying membrane filtration technology |
Non-Patent Citations (1)
| Title |
|---|
| 张丰强: ""丹溪擅用黄柏地龙治痹症"", 《中医临床研究》 * |
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