CN107976478A - A kind of more dyestuff collection method for nucleic acid analysis and its application based on Capillary Electrophoresis - Google Patents
A kind of more dyestuff collection method for nucleic acid analysis and its application based on Capillary Electrophoresis Download PDFInfo
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- CN107976478A CN107976478A CN201711167767.3A CN201711167767A CN107976478A CN 107976478 A CN107976478 A CN 107976478A CN 201711167767 A CN201711167767 A CN 201711167767A CN 107976478 A CN107976478 A CN 107976478A
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- nucleic acid
- electrophoresis
- dyestuff
- collection
- fluorescent dye
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
- G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44782—Apparatus specially adapted therefor of a plurality of samples
Abstract
The present invention relates to area of medical diagnostics, in particular to a kind of more dyestuff collection method for nucleic acid analysis based on Capillary Electrophoresis and its application, including：Before electrophoresis is carried out, spectrum correction is carried out for multiple fluorescent dye collection to be taken respectively, obtains the spectrum correction matrix of each dyestuff collection, and the potting gum method of image capture device is set according to fluorescent dye collection used by each passage product；Nucleic acid to be detected is divided into the segment composition containing multiple nucleic acid fragments, the segment composition is marked at the same time or separately with the multiple fluorescent dye collection and multichannel electrophoresis detection is carried out to the segment composition marked using multiple-pass capillary tube array electrophoresis instrument；The electrophoresis data of each passage are obtained by image capture device, the electrophoresis data gathered using the spectrum correction matrix to each passage carry out spectrum unscrambling.The present invention enables to gene segment analysis experiment more flexible, while has saved time and the cost of gene segment experiment.
Description
Technical field
The present invention relates to area of medical diagnostics, in particular to a kind of more dyestuff collection nucleic acid based on Capillary Electrophoresis
Analysis method and its application.
Background technology
Capillary Electrophoresis (capillary electrophoresis, CE) be ion or charged particle using high voltage electric field as
Driving force, using capillary as split tunnel, according to the mobility or the difference of distribution coefficient between component in sample, and is realized high
Effect, a kind of New Electrophoresis Technique of quick separating.Apparatus includes high voltage power supply, capillary, detector and supplies capillary two
End insertion and two liquid storage bottles being connected with power supply.The separation process of Capillary Electrophoresis is typical differential motion process.Mixing
For thing in transition process, each sample molecule will gradually be divided into different zone because the speed of itself is different, before fast person, after slow person.
Time is longer, and zone is smaller, number is more, distance is more opened, that is, it is better to separate.In a kind of detection of terminal installation of split tunnel
Device, is recorded molecule by the situation of terminal, you can obtain electrophoresis pattern.Using most common capillary zone electrophoresis as
Background electrolyte filled with same composition and same concentrations in example, capillary and slot electrode.Sample from capillary one end (into
Sample end) import, after capillary both ends add certain voltage, charged matter is moved towards the electrode direction opposite with its charge polarity
It is dynamic.Simultaneously as the interface of capillary tube inner wall and buffer solution contact forms electric double layer, cause the solution in capillary additional
Entirety is moved in one direction under the action of electric field, i.e. electric osmose flow phenomenon.Since the speed of electroosmotic flow is faster 5-7 than electrophoretic velocity
Times, therefore Capillary Electrophoresis can be moved positive and negative ion and neutral molecule in one direction using electroosmotic flow together, and ion or
Charged particle migration velocity is the vector sum of electrophoresis and electroosmotic flow speed.Due to the difference of migration velocity between sample each component, warp
Certain time is spent, each component flows out capillary arrival test side by its velocity magnitude and is detected successively, is distributed in time
Electrophoretic image.Make qualitative analysis with the transit time of spectral peak；Quantitative analysis is carried out by the height or peak area of its spectral peak.
Separation analysis of the fast development of life science to biological sample proposes increasingly higher demands.Because biological sample
Species is various, complicated, and sample size is few, prepares, concentrates, separating relatively difficult, analysis heavy workload, so people urgently wish
High throughput, high sensitivity, efficient analysis method are found in prestige.Capillary Electrophoresis is high with its separative efficiency, and analyze speed is fast, sample
The advantages that product dosage is few, automation easy to implement, plays an important role in terms of the analysis of biological sample.Capillary Electrophoresis
Numerous aspects of DNA analysis, such as sequencing, gene mutation analysis, DNA segment or PCR product measure, gene are had application to now
Expression, DNA damage analysis, medical diagnosis on disease etc..And the Direct Analysis of mRNA is relatively difficult, but can also by reverse transcription into
Complementary DNA (cDNA) is analyzed.
But in the prior art, in for DNA segment analysis method, usually experiment can only use a dyestuff collection every time
Sample is marked, the site if desired detected is more, then usually occurs needing to test multiple situation, influence detection when
Effect.
In view of this, it is special to propose the present invention.
The content of the invention
The present invention is set by the potting gum (binning) of spectrum correction combination image capture device, to each passage
In capillary in fluorescence signal carry out spectrum unscrambling, so as in a Capillary Electrophoresis Experiment at the same time using a variety of fluorescence contaminate
Material collection.
Specifically, the present invention relates to a kind of more dyestuff collection method for nucleic acid analysis based on Capillary Electrophoresis, including：
Before electrophoresis is carried out, spectrum correction is carried out for multiple fluorescent dye collection to be taken respectively, obtains each dye
Expect the spectrum correction matrix of collection, and the pixel of image capture device is set according to fluorescent dye collection used by each passage product
Merge (binning) method；
Nucleic acid to be detected is divided into the segment composition containing multiple nucleic acid fragments, by described in segment composition use
Multiple fluorescent dye collection are marked and using multiple-pass capillary tube array electrophoresis instrument to the segment group that has marked at the same time or separately
Compound carries out multichannel electrophoresis detection；
The electrophoresis data of each passage are obtained by image capture device, each passage is adopted using the spectrum correction matrix
The electrophoresis data of collection carry out spectrum unscrambling.
As described above more dyestuff method for nucleic acid analysis based on Capillary Electrophoresis detection the presence or absence of particular locus with
And the application in STR partings.
Compared with prior art, beneficial effects of the present invention are：
By analysis method of the present invention, experiment point can be carried out to the sample using different dyes collection at the same time
Analysis, existing homogencous dyes diversity method need to test multiple situation, only need to be integrated into once experiment i.e. using the present invention
It can complete so that gene segment analysis experiment is more flexible, while has saved time and the cost of gene segment experiment.Using picture
The mode that element merges improves the stability of distribution matrix, reduces calculation amount, improves the stability of detection；Method realization device structure
Simply, it is easy to operate with realizing.
Brief description of the drawings
, below will be to specific in order to illustrate more clearly of the specific embodiment of the invention or technical solution of the prior art
Embodiment or attached drawing needed to be used in the description of the prior art are briefly described, it should be apparent that, in describing below
Attached drawing is some embodiments of the present invention, for those of ordinary skill in the art, before not making the creative labor
Put, other attached drawings can also be obtained according to these attached drawings.
Fig. 1 is the flow diagram of method provided by the present invention；
Fig. 2 is potting gum schematic diagram of the capillary array difference passage using two kinds of dyestuff collection.
Embodiment
The present invention relates to a kind of more dyestuff method for nucleic acid analysis based on Capillary Electrophoresis, including：
Before electrophoresis is carried out, spectrum correction is carried out for multiple fluorescent dye collection to be taken respectively, obtains each dye
Expect the spectrum correction matrix of collection, and the pixel of image capture device is set according to fluorescent dye collection used by each passage product
Merge (binning) method；
Nucleic acid to be detected is divided into the segment composition containing multiple nucleic acid fragments, by described in segment composition use
Multiple fluorescent dye collection are marked and using multiple-pass capillary tube array electrophoresis instrument to the segment group that has marked at the same time or separately
Compound carries out multichannel electrophoresis detection；
The electrophoresis data of each passage are obtained by image capture device, each passage is adopted using the spectrum correction matrix
The electrophoresis data of collection carry out spectrum unscrambling.
Preferably, described image collecting device is charge coupled device (CCD).
The flow of the method for the present invention as shown in Figure 1, the described method includes：
Step 110：Using Capillary Electrophoresis calibration reagent, spectrum correction experiment is carried out for existing dyestuff collection respectively,
Obtain the spectrum correction matrix of each dyestuff collection；
Step 120：PCR amplification is carried out, analysis product is obtained, product is inserted in the sample panel of capillary electrophoresis；
Step 130：The dyestuff collection used according to each passage product, sets potting gum (binning) method of CCD；
Step 140：Capillary Electrophoresis Experiment is carried out, the electrophoresis data of each passage are obtained by CCD imagings；
Step 150：The dyestuff collection used according to each passage, selects corresponding spectrum correction matrix, to original electrophoresis number
According to being corrected, final result is obtained.
Instrument for multicolor fluorescence detection is commonly designed and is calibrated to the combination for very specific fluorescent dye, i.e.,
According to excitation and detection spectrum.
The specificity of detection signal is analyzed to realize, it is necessary to be corrected processing, most general technical method is to establish
Fluorescence signal matrix is combined, the correction of signal strength during for data analysis.Will without too harsh to the intensity signal of fluorescence
Ask, as long as obtaining the accurate position of fluorescence and time signal is obtained with desired experimental result.We are carrying out fluorescence
Spectrum correction is carried out to used known fluorescent dye before detection, i.e., to obtain every kind of mark fluorescent used of laser excitation
Spectrum is individually imaged on CCD, and the distribution matrix of fluorescence spectrum is obtained according to imaging.
It is as follows that the electrophoresis data gathered using the spectrum correction matrix to each passage carry out the step of spectrum unscrambling.
We are set as n kinds it is to be understood that the fluorescent marker that each fluorescent dye used is concentrated has several first.We
Allow n kinds mark fluorescent to be imaged respectively on area array CCD, obtain this spatial distribution of n kind fluorescence on CCD.Area array CCD pixel is
m×p.Every kind of fluorescence is fallen the fluorescence on each pixels of CCD to record by force, obtains the spectrum correction matrix of a m × n.
By spectrum correction matrix, we can carry out spectrum spectrum unscrambling to imaging suite of the fluorescence used on CCD.When we obtain
After having arrived spectrum correction matrix, as long as obtaining the intensity distribution matrix that any time fluorescence falls each pixel on area array CCD, just
The relative intensity distribution of various fluorescence can be obtained by spectrum correction, so as to obtain the spectral information of fluorescence used.
As used in the present invention, in order to improve the stability of spectrum correction matrix, calculation amount is reduced, and cause dyestuff collection
In each dyestuff fluorescence signal relative equilibrium, therefore for dyestuff concentrate fluorescent dye luminosity use corresponding potting gum
Mode, i.e., be a pixel by potting gum adjacent CCD.As potting gum (binning).
Preferably, more dyestuff collection method for nucleic acid analysis based on Capillary Electrophoresis as described above, when the core to be detected
When acid is DNA, the method that nucleic acid to be detected is divided into the segment composition containing multiple nucleic acid fragments is PCR methods.
Preferably, more dyestuff method for nucleic acid analysis based on Capillary Electrophoresis as described above, when the nucleic acid to be detected
For RNA when, first carry out reverse transcription, recycle PCR methods that nucleic acid to be detected is divided into the segment containing multiple nucleic acid fragments and combine
Thing.
Preferably, more dyestuff method for nucleic acid analysis based on Capillary Electrophoresis as described above, the multiple fluorescent dye
The fluorochrome label of concentration is on the locus to be checked in the nucleic acid to be detected.
Preferably, more dyestuff method for nucleic acid analysis based on Capillary Electrophoresis as described above, by the fluorescent dye mark
Remember that the method on the locus to be checked in the nucleic acid to be detected is：By the fluorochrome label one in each locus
5 ' ends of bar primer.
Preferably, more dyestuff method for nucleic acid analysis based on Capillary Electrophoresis, am-plified fragments are nonoverlapping as described above
Locus uses the different fluorescent dye of exciting light using the identical fluorescent dye of exciting light, the overlapping locus of am-plified fragments.
5 ' ends of fluorochrome label primer in each locus, the different fluorescence of different locus primer mark
Marker.Allelic product after amplification carries fluorescence, length allele is separated by electrophoresis, with fluorescent scanning system
To gel detection.According to the colouring discrimination locus of fluorescence, fragment length allele is determined according to the mobility of segment.
Preferably, more dyestuff method for nucleic acid analysis based on Capillary Electrophoresis as described above, the multiple fluorescent dye
The fluorescent dye of concentration includes a variety of in FAM, VIC, NED, PET, LIZ, dR110, TAMRA, ROX, JOE, HEX, TET.
Above-mentioned fluorescence drink composition can be combined according to mode well-known to those skilled in the art, in practical operation
When, the fluorescent dye of different colors can be combined, the launch wavelength difference for combining fluorescent dye is the bigger the better, usually
20-30nm.The method of combination is known to those skilled in the art.Example is the following table is common fluorochrome combinations mode：
The common fluorescent dye collection of table 1
Preferably, more dyestuff method for nucleic acid analysis based on Capillary Electrophoresis as described above, the fluorescent dye collection
The largest passages number of number≤multiple-pass capillary tube array electrophoresis instrument.
In the present invention, according to experiment purpose, identical dyestuff collection can be used in passage portion.Due to current fluorescent dye
Species is limited, at present more fluorescent dye collection using corresponding to G5 and E5 optical filters.
Preferably, more dyestuff method for nucleic acid analysis based on Capillary Electrophoresis as described above, carry out the spectrum correction
When used calibration reagent include IdentifilerTM。
As described above more dyestuff method for nucleic acid analysis based on Capillary Electrophoresis detection the presence or absence of particular locus with
And the application in STR partings.
It is provided by the present invention that the structure of str locus parting collection of illustrative plates is being carried out based on the method for nucleic acid analysis of Capillary Electrophoresis
When, have the advantages that rapidly and efficiently, it is sensitive and accurate.
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the invention.It is not specified in embodiment specific
Condition person, the condition suggested according to normal condition or manufacturer carry out.Reagents or instruments used without specified manufacturer, is
Can be with conventional products that are commercially available.
Embodiment
In one embodiment, 5 color dyestuff collection of existing gene segment analysis mainly have E5 and G5 at present.As shown in Fig. 2,
The potting gum mode of E5 dyestuff collection is uniform, and each pixel that merges arranges for 3 rows 14, totally 20 merging pixels；G5 dyestuff collection
Potting gum mode be non-uniform, each pixel that merges is also 3 rows, totally 20 merging pixels, but each pixel that merges
Columns has differences.
Spectrum correction reagent selects the Identifiler of ABI companiesTMCalibration reagent, implementation steps 110, obtains E5 and G5
Spectrum correction matrix of the dyestuff collection in each passage.Then implementation steps 120, obtain the production to be measured with E5 and G5 fluorescent markers
Thing (method of fluorescent marker can use the primer pair sequence to be checked with E5 and G5 fluorescent markers to be expanded), wherein being placed in logical
Product in road 2 needs to analyze using G5 dyestuffs collection, rest channels product utilization E5 dyestuff set analysis.Used in embodiment
It is 16 Channel Capillary arrays, according to step 130, sets dyestuff in passage 1 to integrate as E5, dyestuff integrates as G5 in passage 2, passage 3
Dyestuff into 16 integrates as E5.Implementation steps 140 and 150, obtain experimental result.
Finally it should be noted that：The above embodiments are only used to illustrate the technical solution of the present invention., rather than its limitations；To the greatest extent
Pipe is described in detail the present invention with reference to foregoing embodiments, but it will be understood by those of ordinary skill in the art that：Its
It can still modify to the technical solution described in foregoing embodiments, either to which part or all technical characteristic
Carry out equivalent substitution；And these modifications or replacement, the essence of appropriate technical solution is departed from various embodiments of the present invention skill
The scope of art scheme.
Claims (10)
- A kind of 1. more dyestuff collection method for nucleic acid analysis based on Capillary Electrophoresis, it is characterised in that including：Before electrophoresis is carried out, spectrum correction is carried out for multiple fluorescent dye collection to be taken respectively, obtains each dyestuff collection Spectrum correction matrix, and the potting gum of image capture device is set according to fluorescent dye collection used by each passage product Method；Nucleic acid to be detected is divided into the segment composition containing multiple nucleic acid fragments, by the segment composition with the multiple Fluorescent dye collection is marked and using multiple-pass capillary tube array electrophoresis instrument to the segment composition that has marked at the same time or separately Carry out multichannel electrophoresis detection；The electrophoresis data of each passage are obtained by image capture device, each passage is gathered using the spectrum correction matrix The electrophoresis data carry out spectrum unscrambling.
- 2. more dyestuff collection method for nucleic acid analysis according to claim 1 based on Capillary Electrophoresis, it is characterised in that work as institute When to state nucleic acid to be detected be DNA, the method that nucleic acid to be detected is divided into the segment composition containing multiple nucleic acid fragments For PCR methods.
- 3. more dyestuff collection method for nucleic acid analysis according to claim 2 based on Capillary Electrophoresis, it is characterised in that work as institute When to state nucleic acid to be detected be RNA, reverse transcription is first carried out, recycles PCR methods that nucleic acid to be detected is divided into containing multiple nucleic acid pieces Disconnected segment composition.
- 4. more dyestuff collection method for nucleic acid analysis according to claim 1 based on Capillary Electrophoresis, it is characterised in that described The fluorochrome label that multiple fluorescent dyes are concentrated is on the locus to be checked in the nucleic acid to be detected.
- 5. more dyestuff collection method for nucleic acid analysis according to claim 4 based on Capillary Electrophoresis, it is characterised in that by institute Stating method of the fluorochrome label on the locus to be checked in the nucleic acid to be detected is：By the fluorochrome label every 5 ' ends of a primer in a locus.
- 6. more dyestuff collection method for nucleic acid analysis according to claim 5 based on Capillary Electrophoresis, it is characterised in that amplification The nonoverlapping locus of segment is marked using identical fluorescent dye, and the overlapping locus of am-plified fragments uses different glimmering Photoinitiator dye is marked.
- 7. according to the more dyestuff collection method for nucleic acid analysis of claim 1,4-6 any one of them based on Capillary Electrophoresis, it is special Sign is, the fluorescent dye that the multiple fluorescent dye is concentrated include FAM, VIC, NED, PET, LIZ, dR110, TAMRA, ROX, It is a variety of in JOE, HEX, TET.
- 8. more dyestuff collection method for nucleic acid analysis according to claim 1 based on Capillary Electrophoresis, it is characterised in that described The largest passages number of the number of fluorescent dye collection≤multiple-pass capillary tube array electrophoresis instrument.
- 9. more dyestuff collection method for nucleic acid analysis according to claim 1 based on Capillary Electrophoresis, it is characterised in that carry out Used calibration reagent includes Identifiler during the spectrum correctionTM。
- 10. more dyestuff method for nucleic acid analysis of claim 1~9 any one of them based on Capillary Electrophoresis are detecting specific base Because of the application in the presence or absence of seat and STR partings.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112513619A (en) * | 2018-09-10 | 2021-03-16 | 株式会社日立高新技术 | Spectrum correction device and spectrum correction method |
| CN112969913A (en) * | 2018-08-31 | 2021-06-15 | 生命科技股份有限公司 | Method and apparatus for single exposure extended dynamic range in capillary electrophoresis |
| CN117448425A (en) * | 2023-12-22 | 2024-01-26 | 北京鑫诺美迪基因检测技术有限公司 | Four-color fluorescence spectrum calibration reagent and preparation method and application thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002023467A1 (en) * | 2000-09-11 | 2002-03-21 | Spectrumedix Corporation | Electrophoretic analysis system having in-situ calibration |
| CN102262081A (en) * | 2011-07-07 | 2011-11-30 | 公安部第一研究所 | Fluorescence spectrum unscrambling method based on matrix analysis |
| CN102411024A (en) * | 2011-06-15 | 2012-04-11 | 公安部第一研究所 | Capillary array electrophoresis detection method based on spatial correction and spectral correction |
| CN202196015U (en) * | 2011-06-15 | 2012-04-18 | 公安部第一研究所 | Capillary array electrophoresis detecting device based on spectrum correction |
| CN104024849A (en) * | 2011-08-12 | 2014-09-03 | 生命科技公司 | Apparatuses, methods, computer program products, and kits for hi-throughput glycan analysis |
| CN105572093A (en) * | 2016-01-27 | 2016-05-11 | 公安部第一研究所 | Multichannel capillary array performance testing device and method |
| CN107338180A (en) * | 2011-10-21 | 2017-11-10 | 尹特根埃克斯有限公司 | sample preparation, processing and analysis system |
-
2017
- 2017-11-21 CN CN201711167767.3A patent/CN107976478A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002023467A1 (en) * | 2000-09-11 | 2002-03-21 | Spectrumedix Corporation | Electrophoretic analysis system having in-situ calibration |
| CN102411024A (en) * | 2011-06-15 | 2012-04-11 | 公安部第一研究所 | Capillary array electrophoresis detection method based on spatial correction and spectral correction |
| CN202196015U (en) * | 2011-06-15 | 2012-04-18 | 公安部第一研究所 | Capillary array electrophoresis detecting device based on spectrum correction |
| CN102262081A (en) * | 2011-07-07 | 2011-11-30 | 公安部第一研究所 | Fluorescence spectrum unscrambling method based on matrix analysis |
| CN104024849A (en) * | 2011-08-12 | 2014-09-03 | 生命科技公司 | Apparatuses, methods, computer program products, and kits for hi-throughput glycan analysis |
| CN107338180A (en) * | 2011-10-21 | 2017-11-10 | 尹特根埃克斯有限公司 | sample preparation, processing and analysis system |
| CN105572093A (en) * | 2016-01-27 | 2016-05-11 | 公安部第一研究所 | Multichannel capillary array performance testing device and method |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112969913A (en) * | 2018-08-31 | 2021-06-15 | 生命科技股份有限公司 | Method and apparatus for single exposure extended dynamic range in capillary electrophoresis |
| CN112969913B (en) * | 2018-08-31 | 2023-08-04 | 生命科技股份有限公司 | Method and apparatus for expanding dynamic range with single exposure in capillary electrophoresis |
| CN112513619A (en) * | 2018-09-10 | 2021-03-16 | 株式会社日立高新技术 | Spectrum correction device and spectrum correction method |
| CN112513619B (en) * | 2018-09-10 | 2024-02-02 | 株式会社日立高新技术 | Spectrum correction device and spectrum correction method |
| CN117448425A (en) * | 2023-12-22 | 2024-01-26 | 北京鑫诺美迪基因检测技术有限公司 | Four-color fluorescence spectrum calibration reagent and preparation method and application thereof |
| CN117448425B (en) * | 2023-12-22 | 2024-03-19 | 北京鑫诺美迪基因检测技术有限公司 | Four-color fluorescence spectrum calibration reagent and preparation method and application thereof |
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