CN1079652A - Production process for culturing bezoar - Google Patents
Production process for culturing bezoar Download PDFInfo
- Publication number
- CN1079652A CN1079652A CN92104330A CN92104330A CN1079652A CN 1079652 A CN1079652 A CN 1079652A CN 92104330 A CN92104330 A CN 92104330A CN 92104330 A CN92104330 A CN 92104330A CN 1079652 A CN1079652 A CN 1079652A
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- China
- Prior art keywords
- calculus bovis
- culture tank
- cattle
- bile
- cultivation
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000004519 manufacturing process Methods 0.000 title claims abstract description 14
- 238000012258 culturing Methods 0.000 title abstract description 6
- 206010004542 Bezoar Diseases 0.000 title abstract 4
- 241000283690 Bos taurus Species 0.000 claims abstract description 26
- 210000000941 bile Anatomy 0.000 claims abstract description 23
- 210000000232 gallbladder Anatomy 0.000 claims abstract description 11
- 210000001198 duodenum Anatomy 0.000 claims abstract description 10
- 206010017631 Gallbladder fistula Diseases 0.000 claims abstract description 5
- 230000004087 circulation Effects 0.000 claims description 12
- 206010016717 Fistula Diseases 0.000 claims description 4
- 230000003890 fistula Effects 0.000 claims description 4
- 201000001883 cholelithiasis Diseases 0.000 claims description 3
- 230000003068 static effect Effects 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 4
- 230000000144 pharmacologic effect Effects 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 3
- 231100000419 toxicity Toxicity 0.000 abstract description 2
- 230000001988 toxicity Effects 0.000 abstract description 2
- 208000035944 Duodenal fistula Diseases 0.000 abstract 1
- BPYKTIZUTYGOLE-IFADSCNNSA-N Bilirubin Chemical compound N1C(=O)C(C)=C(C=C)\C1=C\C1=C(C)C(CCC(O)=O)=C(CC2=C(C(C)=C(\C=C/3C(=C(C=C)C(=O)N\3)C)N2)CCC(O)=O)N1 BPYKTIZUTYGOLE-IFADSCNNSA-N 0.000 description 8
- 239000003814 drug Substances 0.000 description 4
- 238000000338 in vitro Methods 0.000 description 4
- 238000011534 incubation Methods 0.000 description 4
- 239000012141 concentrate Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 210000000013 bile duct Anatomy 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 238000001291 vacuum drying Methods 0.000 description 2
- 206010013786 Dry skin Diseases 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 230000002183 duodenal effect Effects 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 238000005534 hematocrit Methods 0.000 description 1
- 238000001093 holography Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
A production process for culturing bezoar belongs to the technical field of traditional Chinese medicinal materials or biological pharmacy. The process is a complete circulating, continuous and omnibearing calculus bovis culture system which is formed by draining the gall bladder bile of a cow to the outside of a cow body through a gall bladder fistula, culturing the calculus bovis and then feeding the bile in the tank back to the duodenum of the cow through the duodenal fistula. Compared with the existing bezoar cultivation technique in cattle body, it has the advantages of short cultivation time, high yield and capability of being cultivated in factory, and the pharmacological action and toxicity of the product are similar to those of natural bezoar.
Description
This production technology belongs to Chinese crude drug or biological pharmacy technical field.
Calculus Bovis, just the cholelithiasis of cattle is also referred to as natural Calculus Bovis, and it is a kind of rare Chinese medicine, just begins to be used as medicine from ancient times.But, can not satisfy the needs of medical industry or medical treatment for a long time, for this reason because the medicine source electrode is few; 1950's; more domestic R﹠D institutions and pharmacy producer by analyzing the effective ingredient and the pharmacological action of natural Calculus Bovis, have succeeded in developing the artificial Calculus Bovis and have dropped into production; artificial Calculus Bovis's function is similar to natural Calculus Bovis; but the effectiveness of some aspect is more inferior, so the mid-1970s, more domestic units have begun the research of Calculus Bovis cultivating in the cattle body again; during by 1987, very big development has been arranged.Yet Calculus Bovis cultivating is faced with that incubation time is long, output is on the low side relatively in the cattle body, and the content of bilirubin of Calculus Bovis has height to have low wayward and economic benefit not too significantly to wait difficult.Under this technical background; the inventor once invented and had presided over the scientific research of In vitro culture Calculus Bovis in 1987; JIUYUE was applied for patent of invention to Patent Office of the People's Republic of China on 12nd in 1988; number of patent application is: 88 1 06429.7, and the interim technical appraisement of passing through to preside over by Hebei province education commission in Baoding in 26th in December in 1988.Carry out domestic literature retrieval and expert's visit through Institute of Social Science Information of the Chinese Academy of Medical Sciences, do not find the report and the research unit of this respect; Carry out international online retrieval through Chinese science and technology Institute of Social Science Information, do not see the foreign literature data relevant with the present invention.The participant expert is consistent to think that this achievement occupies domestic and international top standard.But it is on the low side that the In vitro culture Calculus Bovis exists the content of bilirubin of product, the technology shortcoming that to be single cultivation go out of use with the bile that causes after the cultivation.
Purpose of the present invention; exactly in order to overcome the shortcoming that In vitro culture Calculus Bovis content of bilirubin bile on the low side, after cultivating goes out of use; and the cultivation time that Calculus Bovis cultivating exists in the cattle body is long and output shortcoming on the low side, and adopts the vanguard technology of holographic bi-circulation culturing system to be used for Calculus Bovis production.
Production technology:
One, the foundation of holographic bi-circulation culturing system:
Use operating method, with bile duct jam plug or with the dual ligation bile duct of suture, flow into duodenal path with blocking-up bile, the excretory bile of cattle liver can only flow into the gallbladder storage like this, again in the special-purpose culture tank of gallbladder fistula this technology that the biliary drainage in the gallbladder is external to cattle, after cultivating, again the bile in the jar is sent back to by the duodenum fistula in the duodenum of cattle, to form complete circulation culture systems (accompanying drawing is a sketch map of the present invention).Holography is meant that whole production technology all designs according to the mechanism that natural Calculus Bovis forms.Two circulations one are meant the mode of Fel Bovis seu Bubali extracorporeal circulation cultivation Calculus Bovis.Two are meant in the special-purpose culture tank of this technology it also is to follow the extracorporeal circulation of Fel Bovis seu Bubali but successive, a continuous multiple incubation.
Concrete operation method does not belong to content of the present invention (but relevant chapters and sections of reference man doctor, veterinary's textbooks such as odonto-maxillary sursery, livestock physiology and pharmacological experimental methodology).
Two, the structure (accompanying drawing is a sketch map of the present invention) of the special-purpose culture tank of this technology;
1. cattle gallbladder fistula.
2. bile entrance branch.
3. motor.
4. bile inlet switch.
5. agitator.
6. bile outlet stool.
7. Calculus Bovis former.
8. Calculus Bovis former turn-knob.
9. bile gate out switch
10. cattle duodenum fistula.
Calculus Bovis former 7, when rotation Calculus Bovis former turn-knob 3, the volume of Calculus Bovis former will shrink and diminish, and is shaped with hematocrit Calculus Bovis composition.
Whole culture tank inwall is to make by non-toxic, colorless glass, brown glass or with non-toxic plastic.
Three, the cultivation of Calculus Bovis:
1. Fel Bovis seu Bubali stops in gallbladder and concentrates: the excretory bile of cattle liver will stop in the gallbladder of cattle 4～12 hours, and bile just has been concentrated like this, by the gallbladder fistula biliary drainage was arrived this culture tank then.
2. what the cattle gallbladder calculus had been suffered from inoculation in this culture tank newly gets 10～50 milliliters of bile as primer.
3. the homoiothermic degree is 36～38 ℃.
4. transfer PH6.7～7.8
5. after beginning to cultivate, preceding 2～6 hours, carry out stir culture with per minute 4～8 commentaries on classics, the back transferred static cultivation in 2～6 hours to.Then, rotate Calculus Bovis former turn-knob, till tightening; connect the duodenum fistula and send the bile in the jar back to duodenum this moment; promptly finished a circulation incubation, and begun the biliary drainage in the cattle gallbladder is cultivated to enter second circulation to this culture tank, so moved in circles.
6. cultivating pressure is an atmospheric pressure.
Four, the gathering and handling of Calculus Bovis:
Each circulation all has the Calculus Bovis composition to form, and the output and the cycle-index of Calculus Bovis are directly proportional, and more than generally cultivating through 30 circulations, just Calculus Bovis can be taken out, and puts into 40 → 60 ℃ of vacuum drying oven inner dryings then to doing, and puts shady and cool dry place and preserves.
This production technology is compared with existing cattle culturing in vivo Calculus Bovis technology; have that incubation time is short, output Gao Bingke carries out characteristics such as batch production cultivation; compare with In vitro culture Calculus Bovis technology, the content of bilirubin with product improves the advantage that the bile after the cultivation is utilized again.With the Calculus Bovis that this technology is cultivated, its pharmacological action and toxicity and natural Calculus Bovis are roughly the same.
Embodiment:
Arrive in this culture tank stopping the biliary drainage that concentrates 4 hours at gallbladder.(1. inoculate 20 milliliters suffer from the cholelithiasis cattle newly get bile.2. the homoiothermic degree is 37 ℃.3. transfer PH6.9).The speed stir culture of changeing with per minute 4 is 4 hours then; change static cultivation 2 hours again over to; rotate then till Calculus Bovis former turn-knob tightens; send the bile in the jar back to duodenum; promptly finishing a circulation cultivates; to in gallbladder, stop the biliary drainage that concentrates 4 hours again then and arrive this culture tank; unclamp the Calculus Bovis former turn-knob of having tightened simultaneously; carrying out second circulation cultivates; after the circulation like this 30 times; can in jar, take out Calculus Bovis at any time, put into vacuum drying oven and be dried to for 50 ℃ and get final product.
Claims (9)
1, a kind of production technology of cultivating Calculus Bovis; it is characterized in that draining in the external culture tank of cattle with the gall bladder bile of gallbladder fistula with cattle; after this jar is cultivated Calculus Bovis; send back to the bile in the jar in the duodenum of cattle by the duodenum fistula again; form a circulation and cultivate, and move in circles with this.
2, the production technology of cultivation Calculus Bovis according to claim 1, it is concentrated after 4～12 hours to it is characterized in that bile will stop in the gallbladder of cattle, just can drain in the external culture tank of cattle.
3, the production technology of cultivation Calculus Bovis according to claim 1, what it is characterized in that in culture tank that inoculation suffers from the cholelithiasis cattle newly gets 10～50 milliliters of bile as primer.
4, the production technology of cultivation Calculus Bovis according to claim 1, when it is characterized in that cultivating Calculus Bovis in the culture tank, preceding 2～6 hours is stir culture, the back was static cultivation in 2～6 hours.
5, the production technology of cultivation Calculus Bovis according to claim 1, the bile in it is characterized in that cultivating behind the Calculus Bovis jar is sent back to again in the duodenum of cattle.
6, a kind of culture tank of cultivating Calculus Bovis that is specifically designed to is characterized in that jar has the Calculus Bovis former.
7, culture tank according to claim 6 is characterized in that the cultivation temperature in the culture tank is 36～38 ℃.
8, culture tank according to claim 6 is characterized in that jar interior PH6.7～7.8.
9, culture tank according to claim 6 is characterized in that the pressure in the culture tank is an atmospheric pressure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN92104330A CN1079652A (en) | 1992-06-06 | 1992-06-06 | Production process for culturing bezoar |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN92104330A CN1079652A (en) | 1992-06-06 | 1992-06-06 | Production process for culturing bezoar |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1079652A true CN1079652A (en) | 1993-12-22 |
Family
ID=4940746
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN92104330A Pending CN1079652A (en) | 1992-06-06 | 1992-06-06 | Production process for culturing bezoar |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1079652A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103945710A (en) * | 2011-09-23 | 2014-07-23 | 自然生物工艺学私人有限责任公司 | Composition |
| CN116492372A (en) * | 2023-06-19 | 2023-07-28 | 内蒙古为旭生物科技有限公司 | Formula of traditional Chinese medicine for feeding lucid ganoderma to form bezoar by cattle and application of formula |
-
1992
- 1992-06-06 CN CN92104330A patent/CN1079652A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103945710A (en) * | 2011-09-23 | 2014-07-23 | 自然生物工艺学私人有限责任公司 | Composition |
| CN116492372A (en) * | 2023-06-19 | 2023-07-28 | 内蒙古为旭生物科技有限公司 | Formula of traditional Chinese medicine for feeding lucid ganoderma to form bezoar by cattle and application of formula |
| CN116492372B (en) * | 2023-06-19 | 2023-09-12 | 内蒙古为旭生物科技有限公司 | Formula of traditional Chinese medicine for feeding lucid ganoderma to form bezoar by cattle and application of formula |
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|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |