CN107936965A - A kind of preparation method with carbon quantum dot with high fluorescence quantum yield and its application in cell imaging - Google Patents
A kind of preparation method with carbon quantum dot with high fluorescence quantum yield and its application in cell imaging Download PDFInfo
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- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 title claims abstract description 81
- 238000002360 preparation method Methods 0.000 title claims abstract description 23
- 238000006862 quantum yield reaction Methods 0.000 title claims abstract description 19
- 238000003384 imaging method Methods 0.000 title claims description 6
- MWUXSHHQAYIFBG-UHFFFAOYSA-N Nitric oxide Chemical compound O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000007787 solid Substances 0.000 claims abstract description 7
- 239000007788 liquid Substances 0.000 claims abstract description 4
- 238000005119 centrifugation Methods 0.000 claims abstract description 3
- 239000000523 sample Substances 0.000 claims abstract description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 24
- 229910052799 carbon Inorganic materials 0.000 claims description 16
- 239000002243 precursor Substances 0.000 claims description 8
- 238000001035 drying Methods 0.000 claims description 7
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 claims description 6
- 238000010438 heat treatment Methods 0.000 claims description 6
- 239000000725 suspension Substances 0.000 claims description 6
- 230000015572 biosynthetic process Effects 0.000 claims description 5
- 238000006243 chemical reaction Methods 0.000 claims description 5
- 238000001514 detection method Methods 0.000 claims description 5
- 235000011837 pasties Nutrition 0.000 claims description 5
- LTUDISCZKZHRMJ-UHFFFAOYSA-N potassium;hydrate Chemical compound O.[K] LTUDISCZKZHRMJ-UHFFFAOYSA-N 0.000 claims description 5
- 239000006228 supernatant Substances 0.000 claims description 5
- 238000003786 synthesis reaction Methods 0.000 claims description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 4
- 235000013878 L-cysteine Nutrition 0.000 claims description 3
- 239000004201 L-cysteine Substances 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 239000002253 acid Substances 0.000 claims description 2
- 229910021529 ammonia Inorganic materials 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 13
- 239000000126 substance Substances 0.000 abstract description 4
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 abstract 6
- 235000018417 cysteine Nutrition 0.000 abstract 2
- 150000001945 cysteines Chemical class 0.000 abstract 2
- 239000008367 deionised water Substances 0.000 abstract 1
- 229910021641 deionized water Inorganic materials 0.000 abstract 1
- 238000010981 drying operation Methods 0.000 abstract 1
- 238000000703 high-speed centrifugation Methods 0.000 abstract 1
- 238000003756 stirring Methods 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 43
- 230000002401 inhibitory effect Effects 0.000 description 11
- 206010028980 Neoplasm Diseases 0.000 description 10
- 201000011510 cancer Diseases 0.000 description 10
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- 238000013459 approach Methods 0.000 description 3
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- 230000010261 cell growth Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
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- 239000010439 graphite Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000002096 quantum dot Substances 0.000 description 3
- 238000004833 X-ray photoelectron spectroscopy Methods 0.000 description 2
- -1 alkane acid amides Chemical class 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 150000001721 carbon Chemical class 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000002161 passivation Methods 0.000 description 2
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- 229920000049 Carbon (fiber) Polymers 0.000 description 1
- 241001212149 Cathetus Species 0.000 description 1
- 206010057249 Phagocytosis Diseases 0.000 description 1
- 241000212749 Zesius chrysomallus Species 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
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- 238000001241 arc-discharge method Methods 0.000 description 1
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- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
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- 238000001311 chemical methods and process Methods 0.000 description 1
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- 239000002086 nanomaterial Substances 0.000 description 1
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- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
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- 229910000033 sodium borohydride Inorganic materials 0.000 description 1
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- 210000001082 somatic cell Anatomy 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
- C09K11/08—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials
- C09K11/65—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials containing carbon
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/001—Preparation for luminescence or biological staining
- A61K49/0013—Luminescence
- A61K49/0017—Fluorescence in vivo
- A61K49/0019—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y20/00—Nanooptics, e.g. quantum optics or photonic crystals
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
Abstract
The present invention relates to a kind of preparation method for the carbon quantum dot fluorescence probe for being used to detect nitric oxide concentration in organism.It is pulverulent solids, there is fabulous water solubility, is synthesized by citric acid and L cysteines by microwave assisting method, it is with high quantum yield, up to more than 85%.Its preparation method is as follows：1) by citric acid and L cysteines dispersing and dissolving in deionized water；2) stirring, ultrasonic disperse make it be uniformly dispersed in the solution；3) it is transferred in micro-wave oven, heats 2~8min；4) it is cooled to room temperature, is dissolved in water；5) centrifuge is moved to, carries out high speed centrifugation；5) liquid obtained after centrifugation is filtered, drying operation, so that carbon quantum dot be made.Gained carbon quantum dot has high-fluorescence quantum yield, good chemical stability, fast light Bleachability and bio-compatibility, is detected suitable for the nitric oxide concentration in organism.
Description
Technical field
The invention belongs to field of nanometer material technology, and in particular to a kind of preparation side with carbon quantum dot with high fluorescence quantum yield
Method and its application in cell imaging.
Background technology
One of carbon is human contact to earliest element, and one of most common element on the earth.Structure on the earth
Elementary cell amino acid, nucleotide into life are exactly to change by skeleton of carbon, are sent out in whole human sciences's technology
Zhan Zhong, is constantly subjected to pay high attention to.Carbon atom has a variety of electron orbits, can form the material of many different structure properties,
So there is a variety of allotropes, such as diamond, graphite, graphene, carbon nanotubes in nature for carbon.Carbon quantum dot
It it is 2004, Xu et al. has been surprisingly found that when purifying single-layer carbon nano-tube, causes the research of the new fluorescent nano material of a wheel
Upsurge.2006, the fluorescent emission of carbon quantum dot greatly improved in Sun et al. by way of being referred to as " surface passivation "
Can, and become a kind of widely used mode.Carbon quantum dot is usually the spheroidal nano-particle of class, comprising indefinite condition or
The core of crystalline state, by graphene and graphene oxide or the graphite with Turbostratic, is formed, its property by the fusion of sp3 hydridization
Matter, element composition are different because of different synthetic methods.At present, in the explanation to carbon quantum dot Fluorescence Mechanism, have two kinds big
Quantity research personnel receive.It is a kind of to explain the fluorescent light source for thinking carbon quantum dot in the band-gap transition on conjugatedπbond, and another
It is considered the surface defect from carbon quantum dot complexity.
Since quantum confined effect and edge effect have preferable fluorescent characteristic, itself can serve as carbon quantum dot
A kind of excellent fluorescence indicator.Simultaneously as carbon quantum dot high-dissolvability in itself and chemical stability, low cytotoxicity,
Good biocompatibility and the characteristic of the resistance to photobleaching good compared to conventional semiconductors quantum dot and organic dyestuff, make it
In fields such as bio-imaging, nanosecond medical sciences, tool has unlimited potential.For example, according to the characteristic of carbon quantum dot and special
Purpose requirement, makes it as a kind of new drug carrier, so as to fulfill the real-time tracing drug diffusion under cell micro-environment can
Depending on change.
In addition, the surface of carbon quantum dot has abundant carboxyl group, make it have good water-soluble and further
Surface modification and the potentiality of passivation, therefore, carbon quantum dot has special development and application advantage in fields such as environment measurings.Compare
In other detection methods, the detection method based on carbon quantum dot not only has higher selectivity but also avoids bio signal
The problem of being obstructed with chemical signal conduction.
The preparation method of carbon quantum dot can be divided into patterning method and polymerization according to the Direction of Reaction to divide.Patterning method is mainly
Using large scale material as carbon source (such as graphite, graphene, carbon nanotubes, carbon fiber and carbon black), pass through physically or chemically side
Method is cut into the carbon quantum dot of small size, and wherein main method has arc discharge method, laser ablation method, electrochemical process and chemistry
Oxidizing process etc..Polymerization generally uses small organic molecule as carbon source, and by polymerizeing, being carbonized etc., chemical process obtains carbon quantum
Point, in this approach the size of carbon quantum dot be generally higher than material as presoma, wherein method mainly including hydro-thermal method,
Microwave method, electrochemical process and combustion method etc..Carbon quantum dot is of less demanding to facility environment, method is simple in building-up process is prepared
Single, speed is quick and reactant selected by it is all green, safety, its manufacturing cost is compared other detection means
It is greatly lowered, being applied to popularization has impetus.
But the quantum yield of the carbon quantum dot synthesized at present is all relatively low, such as：The yellow quantum dot quantum production of Li et al. synthesis
Rate is 11.7%, and after sodium borohydride reduction, quantum yield is just up to 22.9%；The quantum dot of the synthesis such as Mei is passing through
Cross after chemical reaction access alkane acid amides, quantum yield increases to 13% from 0.02%；The amino modified carbon quantum such as Tesuka
Yield reaches more than 40% after point, in addition, the carbon quantum dot of also many variety classes synthesis, but they are far from reaching people
Desired quantum yield.
The content of the invention
The present invention is directed to the deficiencies in the prior art, and it is an object of the present invention to provide a kind of have high-fluorescence quantum yield carbon amount
The preparation method of son point and its application in cell imaging.
For achieving the above object, the technical solution adopted by the present invention is：
A kind of preparation method with carbon quantum dot with high fluorescence quantum yield, includes the following steps：
(1) carbon source is dissolved in the water, obtains precursor solution；
(2) precursor solution is subjected to microwave heating reaction synthesis carbon quantum dot, then cooled to room temperature obtains dark color
Pasty solid；
(3) pasty solid obtained by the step that is dissolved in water (2) obtains suspension；Suspension is centrifuged at a high speed again,
Supernatant is taken, after supernatant liquid filtering, obtains carbon quantum dot solution；
(4) after carbon quantum dot solution is dried, carbon quantum dot is obtained.
In such scheme, the carbon source is monohydrate potassium and L-cysteine.
In such scheme, the concentration of monohydrate potassium is 0.5~1.5mol/L in the precursor solution, half Guang ammonia of L-
The concentration of acid is 0.3~1mol/L.
In such scheme, the power of the microwave heating is 1300W, and the time of microwave heating is 2~10min.
In such scheme, the ultracentrifugal rotating speed is 9000r/min~10000r/min, centrifugation time for 5~
15min。
In such scheme, filtered using pin hole filter, the pin hole filter size is 0.22 μm.
In such scheme, the condition of the drying is：Under vacuum condition, drying temperature is 30 DEG C~80 DEG C, drying time
12h~32h
Above-mentioned preparation method prepares application of the gained carbon quantum dot in cell imaging.The application is specially：By described in
Carbon quantum dot, for detecting nitric oxide concentration in organism, monitors thin in organism in real time as carbon quantum dot fluorescence probe
Born of the same parents' image.
Beneficial effects of the present invention：It is higher with fluorescent yield that preparation method of the present invention prepares gained carbon quantum dot
Advantage, yield are up to more than 85%；It is in human normal cell HUVEC with being presented in the experiment of cancer cell CRL-5288
Superior bio-compatibility, can be successfully entered cell and still show very high fluorescence intensity, in gained carbon quantum dot
(0.07825mg/mL) is close to cell inhibitory rate 0% and the situation for promoting cell growth occurs under best effort concentration.This
Inventing the preparation method has the advantages that simple synthetic method, safety, preparation condition requirement are low, of low cost, prepares gained
Carbon quantum dot is a kind of environmentally friendly novel fluorescent material, to biological cell nonhazardous, without side-effects, is had well
Bio-compatibility, has the function that certain promotion cell growth under suitably concentration, it is possible to achieve active somatic cell in real time into
Picture, new developing direction is provided for biological detection, chemical analysis.
Brief description of the drawings
Fig. 1 is the transmission electron microscope figure of carbon quantum dot prepared by the embodiment of the present invention 1.
Fig. 2 is the fluorescent emission collection of illustrative plates and fluorescence excitation collection of illustrative plates of carbon quantum dot prepared by the embodiment of the present invention 1.
Fig. 3 is the X ray diffracting spectrum of carbon quantum dot prepared by the embodiment of the present invention 1.
Fig. 4 is the x-ray photoelectron spectroscopy of carbon quantum dot prepared by the embodiment of the present invention 1.
Fig. 5 is that photoluminescence intensity change of the carbon quantum dot of the preparation of the embodiment of the present invention 1 under different excitation wavelengths is bent
Line.
Fig. 6 be carbon quantum dot prepared by the embodiment of the present invention 1 using standard fluorescence material as reference, the fluorescence that is calculated
Yield schematic diagram.
Fig. 7 is after carbon quantum dot prepared by the embodiment of the present invention 1 cultivates 12h in human normal cell, with different excitations
Fluoroscopic image obtained by wavelength excitation.
Fig. 8 is that carbon quantum dot prepared by the embodiment of the present invention 1 is trained with various concentrations in human normal cell with cancer cell
Support the cytoactive inhibiting rate curve of 6h.
Fig. 9 be carbon quantum dot prepared by the embodiment of the present invention 1 with various concentrations in human normal cell and cancer cell
Cultivate the cytoactive inhibiting rate curve of 12h.
Figure 10 be carbon quantum dot prepared by the embodiment of the present invention 1 with various concentrations in human normal cell and cancer cell
Cultivate the cytoactive inhibiting rate curve of 48h.
Embodiment
For a better understanding of the present invention, with reference to the embodiment content that the present invention is furture elucidated, but the present invention
Content is not limited solely to the following examples.
Embodiment 1
A kind of preparation method with carbon quantum dot with high fluorescence quantum yield, includes the following steps：
(1) monohydrate potassium 1.5g and L-cysteine 0.57g is taken to be dissolved in 10mL deionized waters, fully dissolving,
Obtain precursor solution；
(2) gained precursor solution is moved into micro-wave oven heating reaction 4min, natural cooling under the conditions of power is 1300W
To room temperature, pasty solid is obtained；
(3) plus pasty solid obtained by 10mL water dissolving step (2) obtains suspension；Again by suspension supercentrifuge
9000r/min is centrifuged 10 minutes, takes supernatant, and supernatant is filtered with 0.22 μm of pin hole filter, filtered solution is collected, is
Carbon quantum dot solution；
(4) it is carbon quantum dot solution is dry (drying temperature is 30 DEG C, drying time 32h) under vacuum, obtain carbon
Quantum dot.
The present embodiment is prepared into gained carbon quantum dot by being compared with standard fluorescence material, according to formula, it is glimmering to calculate its
Quantum yield is up to 85%.Fig. 1 is the transmission electron microscope figure of carbon quantum dot prepared by the embodiment of the present invention 1；Fig. 1 explanations
Obtained carbon quantum dot pattern is approximate circle, and has a clear lattice fringe, forms good lattice structure.Fig. 2
It is the fluorescent emission collection of illustrative plates and fluorescence excitation collection of illustrative plates of carbon quantum dot prepared by the embodiment of the present invention 1, Fig. 2 illustrates obtained carbon
Quantum dot has obvious absworption peak and higher fluorescence intensity.Fig. 3 is that the X of carbon quantum dot prepared by the embodiment of the present invention 1 is penetrated
Ray diffraction diagram is composed, and Fig. 3 illustrates that obtained carbon quantum dot has the structure of amorphous state or crystalline state, and is said with reference to Fig. 1
Understand that obtained carbon quantum dot has crystalline state good, provide the foundation for its high-fluorescence quantum yield.Fig. 4
It is the x-ray photoelectron spectroscopy of carbon quantum dot prepared by the embodiment of the present invention 1, Fig. 4 is illustrated around obtained carbon quantum dot
Exist with a variety of groups, largely exist with the group for improving fluorescence quantum yield around it.
Fig. 5 is that photoluminescence intensity change of the carbon quantum dot of the preparation of the embodiment of the present invention 1 under different excitation wavelengths is bent
Line, Fig. 5 illustrate change of the obtained carbon quantum dot with exciting light, its fluorescence intensity and position also change, it has
Wider emission spectra, to provide possibility in the application of the occasion of different requirements.Fig. 6 is carbon amounts prepared by the embodiment of the present invention 1
Son is put using standard fluorescence material as reference, the fluorescent yield schematic diagram calculated.The slope of Fig. 6 cathetus represents quantum production
Rate, QS are standard fluorescence material (its standard fluorescence yield are 58%), and black side's point and red spots are obtained carbon quantum
Point, its fluorescence quantum yield are up to 85%, are slightly lost after purification, but fluorescence quantum yield still has more than 84%.
Embodiment 2
A kind of cell biological experiment with carbon quantum dot with high fluorescence quantum yield, includes the following steps：
(1) HUVEC cells and CRL-5288 cells are subjected to Nature enemy certain time；
(2) carbon quantum dot obtained in Example 1, is made into various concentrations, two is separately added into after filtration sterilization
In the culture dish of kind cell, cultivated；
(3) situation of carbon quantum dot obtained by cell phagocytosis embodiment 1 is detected under fluorescence microscope；
(4) after 6h, 24h, 48h culture, light absorption value is detected respectively, the carbon quantum as obtained by formula calculates embodiment 1
Inhibiting rate of the point to cell.
It was found from the present embodiment：Under the best effort concentration (0.07825mg/mL) of carbon quantum dot obtained by embodiment 1,
Gained cell inhibitory rate in 6h, 24h, 48h culture duration all to be minimum and below 6%, and as incubation time increases, its
Cell inhibitory rate approaches and reaches 0%, the phenomenon that appearance promotion cell increases.Fig. 7 is carbon quantum prepared by the embodiment of the present invention 1
After point cultivates 12h in human normal cell, with the fluoroscopic image obtained by different excitation wavelength excitations, Fig. 7 is illustrated
After carbon quantum dot obtained by embodiment 1 is used for cell culture, it can not only enter cell, but also cell still large number of viable is simultaneously
Stronger fluorescence is still sent under excitation light.Fig. 8 be the embodiment of the present invention 1 prepare carbon quantum dot with various concentrations in human body
The cytoactive inhibiting rate curve of normal cell and culture 6h in cancer cell；Fig. 9 is carbon quantum dot prepared by the embodiment of the present invention 1
The cytoactive inhibiting rate curve for cultivating 12h in human normal cell and cancer cell with various concentrations；Figure 10 is the present invention
The cytoactive for cultivating 48h in human normal cell and cancer cell with various concentrations of carbon quantum dot prepared by embodiment 1 presses down
Rate curve processed.Fig. 8~Figure 10 illustrates the carbon quantum dot of obtained various concentrations in cancer cell and human normal cell
Influence, show under the optimal use concentration of carbon quantum dot (0.07852mg/mL), it has extremely low suppression to two kinds of cells
Rate processed, in addition after 48h is cultivated already close to and reach control group (using cell culture fluid culture cell) inhibiting rate it is horizontal
(0%), there is the situation for promoting cell growth.In Fig. 9 and Figure 10 it can be found that after 24h and 48h is cultivated, institute in embodiment 1
Obtained carbon quantum dot has the more significant cell inhibitory rate of higher for cancer cell at higher concentrations, and cancer cell is again in order to control
Provide a kind of new approaches.
Obviously, above-described embodiment is only intended to clearly illustrate made example, and is not the limitation to embodiment.It is right
For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of change or
Change.There is no necessity and possibility to exhaust all the enbodiments.And the obvious change or change therefore amplified
Move within still in the protection domain of the invention.
Claims (9)
1. a kind of preparation method with carbon quantum dot with high fluorescence quantum yield, it is characterised in that include the following steps：
（1）Carbon source is dissolved in the water, obtains precursor solution；
（2）Precursor solution is subjected to microwave heating reaction synthesis carbon quantum dot, then cooled to room temperature obtains dark paste
Solid；
（3）Be dissolved in water step（2）Gained pasty solid obtains suspension；Suspension is centrifuged at a high speed again, is taken
Clear liquid, after supernatant liquid filtering, obtains carbon quantum dot solution；
（4）After carbon quantum dot solution is dried, carbon quantum dot is obtained.
2. preparation method according to claim 1, it is characterised in that the carbon source is half Guang ammonia of monohydrate potassium and L-
Acid.
3. preparation method according to claim 1, it is characterised in that the concentration of monohydrate potassium in the precursor solution
For 0.5 ~ 1.5mol/L, the concentration of L-cysteine is 0.3 ~ 1mol/L.
4. preparation method according to claim 1, it is characterised in that the power of the microwave heating is 1300W, and microwave heats
Time be 2 ~ 10min.
5. preparation method according to claim 1, it is characterised in that the ultracentrifugal rotating speed is
9000r/min ~ 10000r/min, centrifugation time are 5 ~ 15min.
6. preparation method according to claim 1, it is characterised in that filtered using pin hole filter, the pin hole mistake
Filter size is 0.22 μm.
7. preparation method according to claim 1, it is characterised in that the condition of the drying is：Under vacuum condition, dry temperature
Spend for 30 DEG C ~ 80 DEG C, drying time 12h ~ 32h.
8. any preparation method of claim 1 ~ 7 prepares application of the gained carbon quantum dot in cell imaging.
9. apply according to claim 8, it is characterised in that using the carbon quantum dot as carbon quantum dot fluorescence probe, use
In detection nitric oxide concentration in organism, organism inner cell image is monitored in real time.
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| CN109749106A (en) * | 2019-01-02 | 2019-05-14 | 齐鲁工业大学 | A kind of fluorescence organic polymer nanometer film and preparation method thereof |
| CN110041923A (en) * | 2019-05-06 | 2019-07-23 | 云南大学 | A kind of preparation method and application of fluorescent carbon quantum dot Phe-CDs |
| CN110129043A (en) * | 2019-02-01 | 2019-08-16 | 中国科学院苏州生物医学工程技术研究所 | The preparation method of carbon quantum dot and kit, the method for detection nucleic acid |
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| CN109749106B (en) * | 2019-01-02 | 2021-06-01 | 齐鲁工业大学 | Fluorescent organic polymer nano film and preparation method thereof |
| CN110129043A (en) * | 2019-02-01 | 2019-08-16 | 中国科学院苏州生物医学工程技术研究所 | The preparation method of carbon quantum dot and kit, the method for detection nucleic acid |
| CN110129043B (en) * | 2019-02-01 | 2022-03-29 | 中国科学院苏州生物医学工程技术研究所 | Preparation method of carbon quantum dots and kit and method for detecting nucleic acid |
| CN110041923A (en) * | 2019-05-06 | 2019-07-23 | 云南大学 | A kind of preparation method and application of fluorescent carbon quantum dot Phe-CDs |
| CN110041923B (en) * | 2019-05-06 | 2022-02-01 | 云南大学 | Preparation method and application of fluorescent carbon quantum dots Phe-CDs |
| CN116478688A (en) * | 2023-03-16 | 2023-07-25 | 长沙矿冶院检测技术有限责任公司 | Carbon quantum dot for detecting mercury ions, synthesis method thereof, mercury ion detection kit and application thereof |
| CN116478688B (en) * | 2023-03-16 | 2024-04-02 | 长沙矿冶院检测技术有限责任公司 | Carbon quantum dot for detecting mercury ions, synthesis method thereof, mercury ion detection kit and application thereof |
| CN116622367A (en) * | 2023-04-04 | 2023-08-22 | 浙江大学 | Composite fluorescent probe for detecting and imaging nitric oxide free radicals and synthesis method |
| CN116622367B (en) * | 2023-04-04 | 2024-04-16 | 浙江大学 | Composite fluorescent probe for detecting and imaging nitric oxide free radicals and synthesis method |
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