CN107929420B - Traditional Chinese medicine composition for improving qi and blood functions and immunity of human body and detection method - Google Patents

Abstract

The invention relates to a traditional Chinese medicine composition for improving qi and blood functions and immunity of a human body and a detection method. Specifically, on the one hand, the invention relates to a traditional Chinese medicine composition which is prepared from 1200 parts by weight of astragalus membranaceus, 400-600 parts by weight of angelica sinensis, 300-500 parts by weight of prepared rehmannia root, 300-500 parts by weight of codonopsis pilosula, 100-300 parts by weight of donkey-hide gelatin, 20-40 parts by weight of ferrous gluconate and optional pharmaceutical excipients. Also relates to a preparation method and a detection method of the traditional Chinese medicine composition. The traditional Chinese medicine composition can improve qi and blood functions of a human body and improve immunity, and has excellent technical effects as described in the specification.

Description

Traditional Chinese medicine composition for improving qi and blood functions and immunity of human body and detection method

Technical Field

The invention belongs to the field of medical health products, relates to a traditional Chinese medicine composition for improving qi and blood functions of a human body and enhancing immunity, and also relates to a preparation process and a detection method of the traditional Chinese medicine composition, in particular to a traditional Chinese medicine composition which is called Hai Rui kang brand astragalus mongholicus and angelica donkey-hide gelatin oral liquid for improving qi and blood functions of the human body and enhancing immunity, and a preparation method and a detection method of the traditional Chinese medicine composition.

Background

Nutritional anemia is divided into small cell anemia and large cell anemia. The former is also called iron deficiency anemia and is mostly developed in infants and children; the latter is also called megaloblastic anemia, whose cause is the lack of factors that mature red blood cells.

In traditional Chinese medicine, nutritional anemia belongs to the category of 'blood deficiency' and 'consumptive disease', blood is considered to come from the spleen, is fundamentally in the kidney and relates to the heart and the liver, and if the spleen and the stomach are weak, food essence cannot be cured, the source of vital energy and blood is insufficient, and anemia is generated. The blood-enriching agent is a kind of prescription based on the theory of "reinforcing the body by deficiency and damaging the body by deficiency" in the "Nei Jing". Therefore, the formula is usually combined with qi tonics to achieve the effect of tonifying qi and generating blood. Cheng is as in Wen Bing tiao Bian (treatise on blood by differentiation and treatment of Wen Bing): for blood deficiency, it can tonify qi and promote blood generation. Kidneys store essence, essence generates marrow, and marrow generates blood, so there is the saying that the source of blood lies in the kidneys. The Hairikang Xueerhong oral liquid is a health food prepared from astragalus root, angelica, prepared rehmannia root, radix codonopsitis, donkey-hide gelatin and ferrous gluconate as raw materials, and has the health care function of improving nutritional anemia as proved by functional tests.

Anemia is a common disease among residents in China and is also a global disease, and the subjects affected by anemia are the most extensive, including children, teenagers, adult men and women, especially pregnant women, nursing mothers and children. The main six manifestations of nutritional anemia: firstly, the onset of the disease is slow, the skin mucous membrane is generally found to be gradually pale, and particularly, the lips and the nail bed are most obvious; fatigue, weakness, malaise, dysphoria or cachexia; thirdly, the appetite is declined, vomiting or diarrhea often occurs, and the pica can occur in some children; the doctor can find the hepatosplenomegaly and the heart rate of the children are increased during examination, and the reduction of hemoglobin is mainly used in the test and examination; patients mostly have puffiness, thin and yellow hair, pale yellow face, fatigue, weakness and inappetence; sixthly, the development of intelligence and action is lagged or even gradually retreated.

Nutritional anemia is one of nutritional deficiency diseases with the highest incidence worldwide, and according to the survey of the world health organization (WTO) on nutritional anemia in 50 hundred million people worldwide, about 10-30% of people worldwide have iron deficiency in different degrees, namely 10 hundred million people. The incidence rate of the anemia is very high in China, and the average incidence rate of the anemia caused by iron deficiency of Chinese residents is 13.6 percent as disclosed in the Chinese resident nutrition and health condition 2004; the prevalence rates of anemia of infants within two years old, the elderly over 60 years old and women of childbearing age are 21.7%, 18.5% and 20.6% respectively; the other group is that the incidence rate of iron deficiency anemia in China reaches 15-20%, the anemia rate of women and children reaches 20% due to iron deficiency, and iron deficiency is listed as one of four nutrient deficiency symptoms in the world by the world health organization (WTO). Therefore, the market demand of blood-enriching products is large.

In addition, it is known that the immunity of the human body can be improved by taking a blood-enriching product.

From the condition of the blood-enriching products in the current market, the blood-enriching products mainly comprise two types: one is chemically synthesized components such as ferrous lactate, porphyrin iron, ferrous sulfate, ferrous citrate, ferrous gluconate, Vc and the like; the other kind is animal and plant and Chinese herbal medicine components such as colla Corii Asini, Mel, gallus Domesticus, fructus Lycii, soybean phospholipid, Ginseng radix, radix Angelicae sinensis, and radix astragali. However, no product for enriching the blood by combining chemical synthesis components, animal and plant components and Chinese herbal medicine components is found in the market.

Therefore, new blood-enriching products and methods are still expected in the art, in particular, products and methods for enriching blood by combining chemically synthesized components with animal, plant and Chinese herbal medicine components; in addition, it is expected that the person skilled in the art will improve the qi and blood functions and enhance immunity based on the way of taking the blood-enriching product.

Disclosure of Invention

The invention aims to provide a blood enriching product and a blood enriching method so as to improve the qi and blood functions of a human body and improve the immunity. Surprisingly, the traditional Chinese medicine composition which can be called as Hai Rui kang astragalus, angelica and donkey-hide gelatin oral liquid and can be used for improving qi and blood functions and improving immunity of a human body has excellent technical effects. The present invention has been completed based on this finding.

In a first aspect, the present invention provides a Chinese medicinal composition, which is prepared from astragalus root, Chinese angelica root, prepared rehmannia root, pilose asiabell root, donkey-hide gelatin, ferrous gluconate and optional pharmaceutic adjuvants.

The traditional Chinese medicine composition is prepared from 1200 parts by weight of astragalus membranaceus, 400-600 parts by weight of angelica sinensis, 300-500 parts by weight of prepared rehmannia root, 300-500 parts by weight of codonopsis pilosula, 100-300 parts by weight of donkey-hide gelatin, 20-40 parts by weight of ferrous gluconate and optional pharmaceutical excipients.

The traditional Chinese medicine composition is prepared from 1200 parts by weight of astragalus membranaceus, 450-550 parts by weight of angelica sinensis, 350-450 parts by weight of prepared rehmannia root, 350-450 parts by weight of codonopsis pilosula, 150-250 parts by weight of donkey-hide gelatin, 25-35 parts by weight of ferrous gluconate and optional pharmaceutical excipients.

The traditional Chinese medicine composition according to the first aspect of the invention is prepared from 1200 parts by weight of astragalus membranaceus, 500 parts by weight of angelica sinensis, 400 parts by weight of prepared rehmannia root, 400 parts by weight of codonopsis pilosula, 200 parts by weight of donkey-hide gelatin, 32 parts by weight of ferrous gluconate and optional pharmaceutical excipients.

The Chinese medicinal composition according to the first aspect of the present invention is a liquid medicament in the form of an oral liquid.

The traditional Chinese medicine composition is a liquid medicament in the form of oral liquid, and is prepared by using 8-16 g of astragalus root medicinal material per 100 ml.

The traditional Chinese medicine composition is a liquid medicament in the form of oral liquid, and is prepared by using 10-14 g of astragalus mongholicus medicine per 100 ml.

The Chinese medicinal composition according to the first aspect of the present invention, which is a liquid medicament in the form of an oral liquid, is prepared in an amount of 12g per 100ml of the astragalus root material used.

The traditional Chinese medicine composition according to the first aspect of the present invention, wherein the pharmaceutical excipient comprises a flavoring agent. In one embodiment, the flavoring agent is a sweetener, such as aspartame, acesulfame k, sodium saccharin, sucrose (e.g., white granulated sugar), and the like. The amount of these sweeteners can be easily determined according to the specific variety of the use and experience of those skilled in the art, for example, the amount of aspartame, acesulfame k, saccharin sodium and the like is usually 0.05% to 1%, especially 0.1%, for example, sucrose (e.g., white granulated sugar) can account for 5 to 25%, for example 10 to 20%, for example 10%, 12%, 15%, 20% of the weight of the Chinese medicinal composition of the present invention. In particular, the amount of sweetener to be used may be determined according to, for example, preferences of the individual user, and the choice of sweetener to be used is well known to those skilled in the art, and its type and amount do not affect the physicochemical properties of the product.

The traditional Chinese medicine composition according to the first aspect of the present invention is a liquid medicament in the form of an oral liquid, which is prepared by a method comprising the steps of:

(1) decocting radix astragali, radix Angelicae sinensis, radix rehmanniae Preparata and radix Codonopsis with purified water twice (for example, decocting 5-8 times (for example, 6 times) of purified water for 1-3 hr (for example, 2 hr), filtering to obtain filtrate, decocting the second residue with 5-8 times (for example, 6 times) of purified water for 1-3 hr (for example, 2 hr), filtering to obtain filtrate), and mixing the two filtrates;

(2) concentrating the combined filtrate under reduced pressure until the weight ratio of the liquid medicine to the medicinal materials is 0.8-1.2: 1, such as 1: 1; adding ethanol (such as ethanol with concentration of 90% or more or 95% or more) to make ethanol content reach 55-65% (such as ethanol content reach 60%), standing for precipitating with ethanol for 20-30 hr, collecting supernatant, and filtering to obtain filtrate;

(3) recovering ethanol from the filtrate obtained in the step (2) at the temperature of 60-80 ℃ under reduced pressure until no alcohol smell exists, and concentrating the alcohol-free liquid under reduced pressure until the relative density is 1.05-1.08 (the measurement temperature is 40 ℃) to obtain a traditional Chinese medicine extracting solution for later use;

(4) smashing donkey-hide gelatin into small pieces, adding 8-12 times, for example, 10 times of purified water, heating to completely dissolve the donkey-hide gelatin, and filtering for later use;

(5) mixing ferrous gluconate and optional flavoring agent (such as sucrose), adding 2-4 times of purified water (such as 3 times of purified water), and heating for dissolving;

(6) mixing the above medicinal liquids of steps (3), (4) and (5), mixing, adding purified water to desired volume, stirring, filtering, packaging, sealing, and sterilizing under hot pressure (such as 105 deg.C for 45 min).

The traditional Chinese medicine composition according to the first aspect of the invention, wherein the pharmaceutic adjuvant further comprises sodium citrate and sorbitol. In one embodiment, the weight ratio of the medicinal materials astragalus root and sodium citrate is 12: 0.1. in one embodiment, the weight ratio of the medicinal materials astragalus and sorbitol is 12: 0.5. in one embodiment, sodium citrate and sorbitol are added to the filtrate obtained in step (2).

Further, the present invention provides a method for preparing a Chinese medicinal composition, for example, the Chinese medicinal composition of any embodiment of the first aspect of the present invention, which is prepared from astragalus root, angelica root, prepared rehmannia root, codonopsis pilosula, donkey-hide gelatin, ferrous gluconate and optionally pharmaceutical excipients; the method comprises the following steps:

(1) decocting radix astragali, radix Angelicae sinensis, radix rehmanniae Preparata and radix Codonopsis with purified water twice (for example, decocting 5-8 times (for example, 6 times) of purified water for 1-3 hr (for example, 2 hr), filtering to obtain filtrate, decocting the second residue with 5-8 times (for example, 6 times) of purified water for 1-3 hr (for example, 2 hr), filtering to obtain filtrate), and mixing the two filtrates;

(2) concentrating the combined filtrate under reduced pressure until the weight ratio of the liquid medicine to the medicinal materials is 0.8-1.2: 1, such as 1: 1; adding ethanol (such as ethanol with concentration of 90% or more or 95% or more) to make ethanol content reach 55-65% (such as ethanol content reach 60%), standing for precipitating with ethanol for 20-30 hr, collecting supernatant, and filtering to obtain filtrate;

(3) recovering ethanol from the filtrate obtained in the step (2) at the temperature of 60-80 ℃ under reduced pressure until no alcohol smell exists, and concentrating the alcohol-free liquid under reduced pressure until the relative density is 1.05-1.08 (the measurement temperature is 40 ℃) to obtain a traditional Chinese medicine extracting solution for later use;

(4) smashing donkey-hide gelatin into small pieces, adding 8-12 times, for example, 10 times of purified water, heating to completely dissolve the donkey-hide gelatin, and filtering for later use;

(5) mixing ferrous gluconate and optional flavoring agent (such as sucrose), adding 2-4 times of purified water (such as 3 times of purified water), and heating for dissolving;

(6) mixing the above medicinal liquids of steps (3), (4) and (5), mixing, adding purified water to desired volume, stirring, filtering, packaging, sealing, and sterilizing under hot pressure (such as 105 deg.C for 45 min).

According to the method of the second aspect of the invention, the Chinese medicinal composition is prepared from 1200 parts by weight of astragalus, 400-600 parts by weight of angelica, 300-500 parts by weight of prepared rehmannia root, 300-500 parts by weight of codonopsis pilosula, 100-300 parts by weight of donkey-hide gelatin, 20-40 parts by weight of ferrous gluconate and optional pharmaceutical excipients.

According to the method of the second aspect of the invention, the Chinese medicinal composition is prepared from 1200 parts by weight of astragalus root, 450-550 parts by weight of angelica, 350-450 parts by weight of prepared rehmannia root, 350-450 parts by weight of codonopsis pilosula, 150-250 parts by weight of donkey-hide gelatin, 25-35 parts by weight of ferrous gluconate and optional pharmaceutical excipients.

According to the method of the second aspect of the present invention, the Chinese medicinal composition is prepared from 1200 parts by weight of astragalus root, 500 parts by weight of angelica, 400 parts by weight of prepared rehmannia root, 400 parts by weight of codonopsis pilosula, 200 parts by weight of donkey-hide gelatin, 32 parts by weight of ferrous gluconate and optionally pharmaceutical excipients.

According to the method of the second aspect of the present invention, the Chinese medicinal composition is a liquid preparation in the form of an oral liquid.

According to the method of the second aspect of the present invention, the Chinese medicinal composition is a liquid preparation in the form of oral liquid, and the amount of the prepared astragalus root is 8-16 g per 100 ml.

According to the method of the second aspect of the present invention, the Chinese medicinal composition is a liquid preparation in the form of oral liquid, and the amount of the prepared astragalus root is 10-14 g per 100 ml.

According to the method of the second aspect of the present invention, the Chinese medicinal composition is a liquid preparation in the form of oral liquid, which is prepared in an amount of 12g per 100ml of the astragalus root material used.

According to the method of the second aspect of the invention, the pharmaceutical excipient comprises a flavoring agent. In one embodiment, the flavoring agent is a sweetener, such as aspartame, acesulfame k, sodium saccharin, sucrose (e.g., white granulated sugar), and the like. The amount of these sweeteners can be easily determined according to the specific variety of the use and experience of those skilled in the art, for example, the amount of aspartame, acesulfame k, saccharin sodium and the like is usually 0.05% to 1%, especially 0.1%, for example, sucrose (e.g., white granulated sugar) can account for 5 to 25%, for example 10 to 20%, for example 10%, 12%, 15%, 20% of the weight of the Chinese medicinal composition of the present invention. In particular, the amount of sweetener to be used may be determined according to, for example, preferences of the individual user, and the choice of sweetener to be used is well known to those skilled in the art, and its type and amount do not affect the physicochemical properties of the product.

According to the method of the second aspect of the present invention, the pharmaceutical excipients further comprise sodium citrate and sorbitol. In one embodiment, the weight ratio of the medicinal materials astragalus root and sodium citrate is 12: 0.1. in one embodiment, the weight ratio of the medicinal materials astragalus and sorbitol is 12: 0.5. in one embodiment, sodium citrate and sorbitol are added to the filtrate obtained in step (2).

Furthermore, in a third aspect of the present invention, there is provided a use of the traditional Chinese medicine composition according to any one of the embodiments of the first aspect of the present invention or the traditional Chinese medicine composition prepared by the method according to any one of the embodiments of the second aspect of the present invention in preparing a product for improving qi and blood functions and enhancing immunity of a human body.

Further, the present invention provides a method for determining the content of calycosin glucoside in the Chinese medicinal composition of any one of the first aspect of the present invention or the Chinese medicinal composition prepared by the method of any one of the second aspect of the present invention, using reversed phase high performance liquid chromatography using Kromasil C18 column (4.6 mm. times.200 mm, 5 μm), acetonitrile-water (28:72) as mobile phase, flow rate of 1.0mL/min, and detection wavelength of 267 nm.

Any embodiment according to any aspect of the invention, wherein the traditional Chinese medicine composition has a formulation as described in any one of the embodiments of the invention.

According to any of the embodiments of any of the aspects of the invention, wherein the Chinese medicinal composition has the formula and the preparation method as described in any of the embodiments of the invention.

In the above-described steps of the preparation method of the present invention, although the specific steps described therein are distinguished in some detail or in language description from the steps described in the preparation examples of the detailed embodiments below, those skilled in the art can fully summarize the above-described method steps in light of the detailed disclosure throughout the present disclosure.

Any embodiment of any aspect of the invention may be combined with other embodiments, as long as they do not contradict. Furthermore, in any embodiment of any aspect of the invention, any feature may be applicable to that feature in other embodiments, so long as they do not contradict. The invention is further described below.

All documents cited herein are incorporated by reference in their entirety and to the extent such documents do not conform to the meaning of the present invention, the present invention shall control. Further, the various terms and phrases used herein have the ordinary meaning as is known to those skilled in the art, and even though such terms and phrases are intended to be described or explained in greater detail herein, reference is made to the term and phrase as being inconsistent with the known meaning and meaning as is accorded to such meaning throughout this disclosure.

The traditional Chinese medicine composition is prepared from astragalus membranaceus, angelica sinensis, prepared rehmannia root, codonopsis pilosula, donkey-hide gelatin and ferrous gluconate.

Astragalus membranaceus (Fisch.) Bunge) is also called Astragalus membranaceus. The main root is thick, woody, branched and grey-white. The stem is upright, the upper part is multi-branched, and the stem has fine edges and is white and soft. Perennial herbs with a height of 50-100 cm. Produced in inner Mongolia, Shanxi, Gansu, Heilongjiang and other places, and is a national third-level protective plant. The astragalus root has been used for more than 2000 years, and has the functions of enhancing the immunologic function of organisms, protecting the liver, promoting urination, resisting aging, resisting stress, reducing blood pressure and having wider antibacterial action. But excessive superficial pathogenic factors, qi stagnation and dampness obstruction, food stagnation, excessive heat-toxicity at the early stage of superficial infection or after ulceration, and yin deficiency and yang hyperactivity are prohibited. Due to the long-term mass mining, the number of wild astragalus roots is sharply reduced in recent years, and the danger of extinction is prone to exist. Therefore, the plant is determined to be a gradually dangerous species, and the national third-level protection plant.

Huang Qi is sweet in nature and warm in taste, and enters lung, spleen, liver and kidney meridians.

The medicinal value of astragalus includes: 1. spontaneous sweating due to exterior deficiency: it is usually indicated for spontaneous sweating due to weak body and exterior. For example, Huang Qi combined with Bai Zhu and Fang Feng should be used for sweating due to insecurity of exterior qi, and it is effective after long-term administration. As for jade screen powder; it can also be combined with light wheat and radix Ephedrae. 2. Night sweat due to yin deficiency: it is combined with yin-nourishing herbs such as Sheng Di and Mai Dong, etc. 3. Edema due to acute nephritis: it is indicated for edema of deficiency type due to insufficiency of yang qi, and often combined with Fangji, Fu Ling and Bai Zhu, for instance, Fang Ji Huang Tang. 4. Edema due to chronic nephritis, deficiency of spleen and kidney: it is often combined with Dang Shen, Bai Zhu and Fu Ling. 5. Deficiency of yang-qi: it can be used for treating sore and ulcer without rupture and invagination, and has effects of promoting rupture and limiting. For long-time abscess and deep-rooted carbuncle without penetrating head, it is often combined with pangolin scales, Chinese honeylocust spine, Dang Gui and Chuan Xiong. 6. Ulceration of sores: for long-term unhealing, it is usually combined with Yin Hua, Zao Ci and Di Ding, etc., to promote tissue regeneration and heal up. For pus cleansing, it is combined with Dang Shen and Rou Gui. 7. Lung qi deficiency syndrome: cough and dyspnea with a long time, shortness of breath, mental fatigue, phlegm obstructing in the lung and failing to expectorate. It is combined with herbs of warming lung and relieving asthma and strengthening lung qi, such as Zi Wan and Fang Dong, etc. Spleen producing phlegm and lung storing phlegm, so it is good at strengthening taiyin to dispel phlegm, and Huang Qi is good at tonifying qi to treat qi deficiency. 8. Deficiency of vital energy: lassitude, hypodynamia, or sinking of the middle-warmer, rectocele, and prolapse of uterus. For tonifying qi and strengthening spleen, it is often combined with Dang Shen and Bai Zhu; for qi-tonifying and yang-raising with sinking of qi, it is often combined with Dang Shen, Sheng Ma, chai Hu and gan Cao-Zhi.

The preparation is clinically applied to: 1. hypertension; 2. ischemic heart disease; 3. acute glomerulonephritis; 4. positive gastric ulcer of helicobacter pylori; 5. psoriasis; 6. diabetes mellitus; 7. chronic rhinitis; 8. osteoporosis.

Astragalus membranaceus is a pure natural product which is frequently eaten by people, and smooth running of 'frequently drinking astragalus soup, preventing diseases and keeping health' is carried out in folk, which means that astragalus membranaceus and Qiongzhen lucid ganoderma are frequently used for decocting or soaking in water to replace tea for drinking, and the astragalus membranaceus has good disease prevention and health care effects. Radix astragali and ginseng both belong to good qi-tonifying drugs, and ginseng is mainly used for tonifying primordial qi and restoring yang from collapse, and is commonly used for treating acute diseases such as collapse, shock and the like with good effect. While Huang Qi is mainly tonifying deficiency, it is commonly indicated for chronic weakness, low speech and weak pulse. Some people are easy to catch cold when they meet weather change, the traditional Chinese medicine is called as 'exterior insecurity', astragalus can be used for strengthening exterior, and frequent taking of astragalus can avoid frequent cold.

Modern medical research shows that astragalus has the functions of enhancing the immunity of organisms, protecting the liver, promoting urination, resisting aging, resisting stress, reducing blood pressure and resisting a wide range of bacteria. Can eliminate albuminuria caused by experimental nephritis, enhance myocardial contraction force, and regulate blood sugar content. Radix astragali can not only dilate coronary artery, improve myocardial blood supply, and enhance immunity, but also delay cell aging process. The astragalus root is convenient to eat and can be used for decocting soup, decocting paste, soaking wine, adding into dishes and the like.

Astragalus root has the functions of invigorating qi, promoting the production of body fluid, and promoting the production of blood, and can mutually enhance the curative effect when being reinforced mutually. However, ginseng has strong action, is known as the first important herb for tonifying qi, and has the effects of tonifying qi, relieving collapse, soothing the nerves, improving intelligence, tonifying qi and supporting yang. Dang Shen is mild in action of tonifying qi, so it is specially indicated for tonifying qi of spleen and lung, and tonifying blood. Huang Qi is weaker than ren Shen in tonifying original qi, but it is good at tonifying qi and raising yang, tonifying defensive qi and consolidating superficial resistance, promoting tissue regeneration, inducing diuresis and alleviating edema, especially indicated for spleen deficiency and qi sinking and exterior deficiency with spontaneous sweating.

The chemical components of astragalus root mainly contain glycosides, polysaccharides, flavones, amino acids, trace elements, etc.

Prepared rehmannia root, original name: rehmannia, also known as: radix rehmanniae and radix rehmanniae Preparata. Latin name: rehmannia glutinosa (Gaetn.) libosh.ex fisch.et mey. scrophulariaceae, Rehmannia, dense-covered white, multicellular, long, velvety and glandular hairs. The rhizome is used as a medicine. The prepared rehmannia root, a traditional Chinese medicine, is a processed product of rehmannia root, has black and glossy surface, soft and flexible quality, sweet taste and mild nature, and has the effects of nourishing yin and supplementing blood, replenishing vital essence and replenishing marrow and the like. Can be used for treating deficiency of liver-yin and kidney-yin, soreness of waist and knees, hectic fever, night sweat, nocturnal emission, internal heat, diabetes, blood deficiency, sallow complexion, cardiopalmus, menoxenia, giddiness, tinnitus, and premature gray hair. The dried rehmannia root is prepared rehmannia root which is fumigated to be black and moist, is irregular block-shaped and has different sizes, the inside and the outside are painted black, and the product has luster and the outer surface is wrinkled and uneven. Has moist cross section, bright grease-like block in the center, high viscosity, soft texture, slight smell, and sweet taste. It is better to have a big, soft and moist root tuber, and black and glossy interior and exterior through nine times of steaming and sun drying.

The nature, flavor and meridian tropism. Sweet in taste; and (4) temperature. Liver diseases entering; the kidney channel. The efficacy is mainly as follows: enriching blood and moistening; replenishing vital essence and marrow; for sallow complexion due to blood deficiency; dizziness and palpitation; menoxenia; collapse is not stopped; deficiency of liver-kidney yin; tidal fever and night sweats; spermatorrhea and impotence; infertility; menoxenia; metrorrhagia and metrostaxis and bleeding; soreness and weakness of waist and knees; tinnitus and deafness; dizziness and blurred vision; the beard and hair are early white; quenching thirst; constipation; dyspnea and tachypnea due to kidney deficiency.

Rehmannia has a wide range of pharmacological actions. The comparison of the pharmacological effects of rehmanniae radix Preparata and rehmanniae radix upon the intravascular thrombotic syndrome shows that: the crude radix rehmanniae Preparata of pig gallbladder shape produced in China can strongly inhibit hemorrhagic necrosis of liver and simple necrosis. The antithrombin effect of rehmanniae radix preparata is weak. The activation of the fibrinolytic system was studied by the activation of plasminogen using the fibrin plate method, and it was found that rehmanniae radix had the activation but rehmanniae radix did not. It is also reported that the hemostatic action of rehmannia root before and after charring is not enhanced. The hemostatic effect of the water decoction of rehmannia root, prepared rehmannia root, rehmannia root charcoal and prepared rehmannia root charcoal is analyzed, and the result has no significant difference. The experiment shows that: the Huaiqing prepared rehmannia root decoction can relieve the weight loss of hyperthyroidism type yin-deficiency rats after being infused with water, obviously reduces the water intake and urine volume in 24 hours, obviously improves the concentration levels of T3 and T4 in blood plasma (namely, reduces T3 and increases T4) and obviously increases the level of AD (aldosterone in blood plasma). Proved that the medicine has stronger effect of nourishing yin. It is thought that the yin-nourishing and kidney-tonifying effects of rehmanniae radix Preparata are related to improving AD level in vivo.

The prepared rehmannia root decoction can obviously increase the activity of glutathione peroxidase (GSH-Px) in animal serum when being irrigated to a mouse; reduce the content of lipid peroxide in serum. Can increase the activity of superoxide dismutase (SOD) in blood to a certain extent, but has no statistical significance. Has no influence on the catalase activity in blood. Radix rehmanniae Preparata has certain effect on protein metabolism of rat viscera. Radix rehmanniae Preparata orally has the effect of inhibiting mitosis of epithelial cells. Experiments show that: the Liuwei Dihuang decoction taking prepared rehmannia root as a main drug can increase the weight of a normal mouse, prolong the swimming time and enhance the physical strength, can reduce the induction rate of mouse anterior squamous cell carcinoma caused by N-nitrososarcosine ethyl ester, enables the spleen lymph nodule of an animal receiving a chemical carcinogen to grow and activate the proliferation of a center of hair growth, can enhance the phagocytic activity of a mononuclear macrophagy system at the initial stage of inoculation of a transplanted tumor, improves the proportion of albumin and globulin in the blood of the tumor-bearing animal, and can similarly prolong the survival time of the tumor-bearing animal. Firstly, the prepared rehmannia root has the effect of delaying the aging of mice: the 20% water decoction of radix rehmanniae Preparata is administered by intragastric administration for 45 days, and blood is sacrificed and blood is taken out, and activity of superoxide dismutase (SOD), Catalase (CAT), glutathione peroxidase (GSH-Px) and content of Lipid Peroxide (LPO) are determined. As a result, rehmanniae radix Preparata can enhance GSH-Px activity and reduce LPO content in serum, which is P <0.05 and P <0.01, respectively, compared with control group. The effect on hyperthyroidism type yin deficiency rats is as follows: administration of triiodothyronine (T3) to rats resulted in a yin deficiency model and 3ml (70% concentration) of yellow water decoction of rehmannia glutinosa for 6 days, after which each data was measured. Results compared to the administered group, the normal control group had no matter signs, weight change, 24-hour water intake and urine intake, and related hormones: there were no significant differences in plasma concentrations of T3, thyroxine (T4) and Aldosterone (AD) (P > 0.05). However, after the model is made, the yin-deficiency group has very obvious changes compared with the normal group, such as good excitement, weight loss, increase of 24-hour drinking amount and drinking amount, increase of T3 concentration, decrease of T4 and AD concentration and the like. The symptoms and indexes of the yin-deficiency rats are obviously improved after the radix rehmanniae preparata is fed, and the symptoms and indexes are normal (compared with the yin-deficiency group). For example, compared with the group with yin deficiency and radix rehmanniae preparata, the AD concentration in blood plasma is obviously increased (P <0.05) and the urine volume is obviously reduced (P <0.01) in 24 hours, which indicates that the radix rehmanniae preparata has the function of nourishing kidney yin and is related to the improvement of the AD level in vivo. The concentrations of T3 and T4 in the plasma of the yin-deficiency administration group are obviously improved (the concentration of T3 is reduced, and the concentration of T4 is increased), and the concentration is normal. The prepared rehmannia root can improve the symptom of yin deficiency through the systemic regulation effect and can regulate the abnormal thyroxine state. In addition, compared with the normal group, the yin deficiency administration group still significantly did not reach the normal level in terms of weight change, 24-hour water intake, plasma T3, T4 concentration and the like, and all were significantly different (P < 0.01). This indicates that the index of the group administered with yin deficiency was significantly improved and tended to be normal, but not completely restored to normal, as compared with the group administered with yin deficiency. Indicating that the action of prepared rehmannia root is still limited. Experimental observation of the blood replenishing effect of prepared rehmannia root: preparing raw and cooked rehmannia glutinosa Libosch into a raw and cooked rehmannia glutinosa Libosch powder 1: the decoction 1 is administered by intragastric administration to mice to observe the effects on hemorrhagic anemia, hematopoietic stem cells and CFU-E production of myeloid erythroid progenitor cells. As a result: the fresh and cooked rehmannia has obvious effect on blood loss mice, after 8 days of administration, the prepared rehmannia root group recovers quickly and basically, and the P values of RBC and Hb counts are 0.001 and 0.01 respectively compared with the P values of a control group. Raw and prepared rehmannia root also has certain proliferation and differentiation effects on hematopoietic stem cells, and similarly, the blood enriching effect of rehmannia root is related to the generation of the hematopoietic stem cells for promoting blood cells. Production of hematopoietic cells from mouse bone marrow erythroid: the number of erythroid colony in normal saline group is 52.4 + -18.41, and the number of radix rehmanniae and radix rehmanniae Preparata groups is 60.2 + -19.44 and 125 + -20.45 respectively (P < 0.05). It is suggested that the blood-enriching action of rehmannia root is closely related to the hematopoietic system of bone marrow. The influence on the hemostasis effect after the rehmannia is fried to charcoal: rehmanniae radix, charred radix rehmanniae, radix rehmanniae Preparata, and charred radix rehmanniae Preparata are prepared into 100g crude drug or 33g charred drug per 100ml decoction respectively. Each sample was gavaged to mice at a dose of 0.8ml/20g, and blood was taken from the inner canthus of the left eye, retrobulbar venous plexus, and blood clotting times were counted and compared to saline group. The results have no significant difference (P >0.05), and indicate that the rehmannia root does not need to be processed for hemostasis.

In the aspect of Chinese medicine, Chinese angelica is regarded as the essential herb for blood because it has sweet and heavy flavor, and can tonify blood, and has light and pungent qi. Therefore, it can tonify blood, activate blood, dredge channels and activate collaterals. Radix Angelicae sinensis can be used for treating menoxenia, dysmenorrhea, blood deficiency, amenorrhea, sallow complexion, asthenia anemia, metrorrhagia, puerperal blood stasis, menorrhea (bleeding of mouth and nose during menstruation). The angelica is the most common medicine in the traditional Chinese medicine and is mostly used for cooking soup, and is particularly best for anemia patients. Can also remarkably promote the hematopoietic function of the organism and increase the content of red blood cells, white blood cells and hemoglobin. In terms of enriching and nourishing blood, angelica is sweet and heavy in flavor, so it is the first herb for enriching blood and indicated for pale or sallow complexion, lassitude, hypodynamia, pale and lusterless lips and nails, dizziness, palpitation and insomnia due to heart-liver blood deficiency. The Chinese angelica is combined with prepared rhizome of rehmannia, white peony root and Szechuan lovage rhizome to form a representative blood-enriching prescription, namely 'Siwu decoction', which has the functions of regulating menstruation and enriching blood and is particularly suitable for regulating and treating postpartum blood deficiency. In the aspect of relaxing bowel, traditional Chinese medicine considers that essence and blood are homologous, the body fluid of blood-deficient patients is insufficient, and constipation is easily caused by intestinal fluid deficiency. Dang Gui can moisten intestines to relieve constipation, and is often combined with Ma ren, Ku xing ren and Da Huang to treat constipation due to blood deficiency; the medicine can be combined with radix Saposhnikoviae, rhizoma Ligustici Chuanxiong, radix Paeoniae, radix et rhizoma Rhei, folium Menthae, herba Ephedrae, etc. to make into FANGFENGTONGSHENG powder with effects of clearing heat and relaxing bowels. In the aspect of regulating menstruation and activating blood, the angelica sinensis is light and pungent in qi, so the angelica sinensis can move blood, can clear and regulate the channels, activate collaterals and relieve pain, is particularly suitable for women, is particularly suitable for diseases such as irregular menstruation, dysmenorrheal, blood deficiency amenorrhea and the like, and is called as gynecological eriodictyon by ancient people. It is combined with peach kernel, safflower, prepared rehmannia root, white peony root and ligusticum wallichii to form a famous gynecological formula-Taohong Siwu Tang which is commonly used for premenstrual and dysmenorrhea. In the aspect of the effect on uterine smooth muscle, the angelica volatile oil has the inhibition effect on isolated uterus, enables rhythmic contraction to be in a relaxation state and resists uterine contraction. In the aspect of anticancer effect, Dang Gui can be widely used for various tumors, especially gynecological tumors, and is most suitable for stagnation of qi and blood and accumulation of blood stasis. For middle and late stage cancer with blood deficiency and emaciation or patients with deficiency of vital qi after operation, radiotherapy and chemotherapy, radix Angelicae sinensis can also be selected to strengthen body resistance and resist cancer. In the aspect of anti-aging, the angelica decoction has obvious influence on the learning and memory of mice, and the angelica can improve dementia caused by aluminum trichloride, reduce the peroxidized ester level of the brain of a dementia mouse and the oxidation activity of B-type monoamine and treat senile dementia by using Y maze method. In the aspect of preventing and treating chilblain, the traditional Chinese medicine thinks that although the chilblain is on the skin, the chilblain is mainly caused by insufficiency of yang qi of a body, invasion of exogenous cold, failure of yang qi to stretch and congealing cold and blood stasis. Therefore, it is usually treated by warming channel to dispel cold, promoting blood circulation to remove blood stasis, and relieving swelling and pain. Angelica sinensis, ginger and mutton soup is from treatise on miscellaneous diseases due to exogenous pathogenic factors. In the formula, the angelica has the effects of nourishing blood and activating blood, the ginger has the effects of warming yang and dispelling cold, and the mutton has the effects of tonifying deficiency and generating blood. In the aspect of immune function, the angelica sinensis and the sodium ferulate and the angelica sinensis polysaccharide which are extracted from the angelica sinensis have obvious stimulation effect on a mononuclear-macrophage system and have immune regulation and recovery effect on organisms with low immune function. Angelica sinensis can also promote lymphocyte transformation of healthy people. Dang Gui is good but contraindicated in Dang Gui, which should be taken carefully. Many people can eat angelica sinensis, but proper amount of the angelica sinensis needs to be taken, and if the angelica sinensis needs to be taken, side effects are easy to generate. Excessive oral administration of the angelica decoction and the powder can be disappeared after the medicine is stopped, if the powder is occasionally reacted with fatigue, somnolence and the like. The angelica volatile oil can be injected into acupoints to relieve fever, headache, dry mouth, nausea, etc. The blood pressure is reduced when a large dose is administered, and the blood pressure is suddenly reduced when the dose is increased, so that the breathing is stopped. The angelica ether extract has strong toxicity, and a small amount of the angelica ether extract can cause death. The clinical use of Dang Gui should not be excessive, and should be noticed after taking medicine for adverse reaction. Therefore, it is preferable to take the medicine as prescribed.

Dang Gui has various pharmacological actions. (1) The Chinese angelica aqua has the function of resisting thrombosis, and the Chinese angelica aqua has obvious inhibiting effect on the formation of artery and vein bypass thrombosis of rats by intravenous injection or oral administration. (2) Improving blood circulation, and increasing the diameter and blood flow velocity of pia mater arteriole after Middle Cerebral Artery Occlusion (MCAO) operation by butyl phthalide in radix Angelicae sinensis, thereby improving pia mater microcirculation. The apoptosis of neurons exists in the cerebral ischemia process, and the butylphthalide can weaken or stop the low-sugar hypoxia-induced apoptosis process of nerve cells and prevent the cerebral infarction area from expanding. (3) The radix Angelicae sinensis decoction or fluid extract has effect in inhibiting isolated Bufo siccus heart, and stopping heartbeat in diastole when dosage is large. The angelica has a protective effect on arrhythmia of myocardial ischemia reperfusion of rats, and 0.6g/kg of angelica injection is used for intraperitoneal injection of rats, so that the incidence rate of premature beat and the total incidence rate of arrhythmia are obviously reduced. (4) Improving coronary circulation, and intravenous injection of radix Angelicae sinensis decoction (2g/kg) can significantly increase coronary flow, reduce coronary resistance and myocardial oxygen consumption, increase cardiac output and cardiac index, and relieve myocardial infarction when blocking coronary artery. (5) Has effects in dilating blood vessel and relieving spasm due to Noradrenaline (NA). The angelica volatile oil is the main active part of angelica playing a role in relieving spasm of vascular smooth muscle, wherein ligustilide has the strongest activity. The angelica volatile oil also has strong inhibition effect on intestinal smooth muscle spasm. (6) The angelica has obvious inhibition effects on acute capillary permeability increase, tissue edema and chronic injury caused by various inflammatory agents, can inhibit granulation tissue proliferation in the later stage of inflammation, does not influence the weight of adrenal gland and thymus, and suggests that the anti-inflammatory effect of the angelica is independent of a pituitary-adrenal system. The water extract of angelica shows analgesic effect on writhing reaction caused by intraperitoneal injection of acetic acid, and the analgesic effect strength is 1.7 times of that of sodium acetylsalicylate. (7) The Chinese angelica powder (1.5g/kg) is orally taken, has the function of reducing the blood sugar of experimental hyperglycemia of rats and rabbits, and is realized by promoting the secretion of insulin. (8) Has lung protecting effect, and radix Angelicae sinensis can dilate pulmonary artery of rat, and reduce acute hypoxic pulmonary hypertension. The angelica has a certain blood pressure reducing effect on pulmonary hypertension of chronic obstructive pulmonary disease. (9) The angelica can increase the weight of solid matters in the bile and the discharge amount of cholic acid, and protect the activities of ATP enzyme, glucose-6-phosphatase and 5-nucleotidase, which indicates that the angelica has certain functions of protecting liver cells and restoring certain functions of the liver. (10) The angelica has certain protection effect on the kidney, can improve the glomerular filtration function and the renal tubule reabsorption function of rabbits after renal ischemia, relieves renal damage and promotes the recovery of renal tubule lesions. (11) Blood enriching effect, the angelica polysaccharide can activate macrophages, lymphocytes and the like in a hematopoietic microenvironment through direct and/or indirect ways, and can also stimulate muscle tissues to generate hematopoietic regulatory factors, further promote the proliferation and differentiation of functional hematopoietic stem cell granulocyte macrophage colony forming unit (CFU-GM) and stimulate myelogranulocytic monophylesis. In addition, angelica also exhibits the following effects: has effects in treating uterine smooth muscle, cardiovascular system, blood system, inflammation, nervous system, and asthma. In addition, in the aspect of clinical application, the angelica can treat muscle and joint pain and neuralgia, chronic tracheitis, chronic pelvic inflammatory disease, menstrual disease, hypertension, herpes zoster and rhinitis.

Codonopsis pilosula (scientific name; Codonopsis pilosula (Franch.) Nannf.) of Campanulaceae, perennial herb. The main effect of dangshen is to tonify qi. It is most suitable for treating listlessness, asthenia, and lassitude. Low voice, short subjective breathing, and dyspnea with short breath. For those with the emphasis on qi deficiency of spleen and stomach, there are general weakness, poor appetite, and loose stool, Dang Shen should also be used. Radix Codonopsis is usually combined with Atractylodis rhizoma, Poria and radix Glycyrrhizae Preparata, which is named formula Sijunzi decoction for invigorating qi and spleen. For both lung qi and spleen qi deficiency, it is also indicated to use Dang Shen in combination with Huang Qi, Bai Zhu, Fu Ling, Chen Pi, Dang Gui, Sheng Ma, chai Hu, gan Cao, Sheng Jiang and Zi Zao, which is a famous decoction for tonifying middle-jiao and Qi.

Dangshen has the great characteristics of benefiting qi and nourishing blood. So that qi and bloodRadix Codonopsis is also suitable for people with deficiency of both qi, short breath, palpitation, fatigue, asthenia, pale complexion, dizziness, poor appetite, loose stool, and common cold. Experiments prove that the codonopsis pilosula can increase red blood cells and red bloodEggThe white blood is increased, so the codonopsis pilosula is beneficial for anemia patients to eat. The radix Codonopsis has effect in promoting leukocyte increase due to leukocyte decrease caused by chemotherapy and radiotherapy. In recent years, it has been found that the combination of dangshen, astragalus and atractylodes can reduce the urine protein of chronic nephritis patients.

The actions of Dang Shen include many aspects. Specifically, (1) influence on blood cells: alcohol and water extract of radix Codonopsis can be administered orally or subcutaneously to increase red blood cells and hemoglobin of normal rabbit slightly; since the action is remarkably weakened after the removal of the spleen, it is presumed that the blood-replenishing action is related to the spleen, and at this time, the leukocyte tends to be reduced. Radix codonopsis immersion liquid 1: 40 has no hemolysis in the test tube, but can change color after reacting with erythrocyte, and generates turbidity and precipitation. (2) Effects on blood glucose: the codonopsis pilosula extract is injected subcutaneously on the abdomen of the rabbit, so that the blood sugar can be increased; however, if the fermented extract is injected or administered by gastric lavage, the effect is not good, so the blood sugar is considered to be increased because the root contains a large amount of sugar; it also does not inhibit the development of neurogenic hyperglycemia that occurs from diuretic injections. According to preliminary experiments, the radix codonopsis pilosulae can increase blood sugar by intragastric administration. (3) The function of reducing blood pressure: the injection of alcohol and water extract is intravenous or intraperitoneal, which can reduce the blood pressure of the anaesthetized dog, and according to the preliminary analysis, the injection is considered to be peripheral; and has some anti-adrenergic effects; the water extract or alcohol or water extract can also reduce blood pressure of anesthetized animals, and inhibit isolated toad heart. For chronic hypertension dogs, 4g (crude drugs)/kg of the water extract is infused every day, and the blood pressure reducing effect is not seen. The extract or total glycoside of root of plants of the same genus can increase erythrocyte application (17.5%) and increase body weight (23%) when feeding rabbits for a long time (40 days); has effects of preventing and treating leukocytosis caused by turpentine; after long-term oral administration, no pathological changes are found in the internal organs. (4) Effects on the center: the radix Codonopsis decoction can be used for resisting convulsion of mice caused by Nitrosostenin, prolonging convulsion occurrence time, prolonging survival time of mice, and resisting electroconvulsive. The radix Codonopsis decoction can remarkably resist mouse memory disorder caused by scopolamine; it also has effect in resisting memory reproduction disorder of mice caused by ethanol. (5) Effect on cardiovascular system: the ethanol extract and water extract have blood pressure lowering effect due to peripheral vasodilatation, and the ethanol extract has effect in inhibiting isolated Bufo siccus heart. (6) Effect on blood: the codonopsis pilosula water-soluble substance is used for gastric lavage, and has an inhibitory effect on platelet aggregation of rats induced by ADP. The radix Codonopsis product can significantly increase red blood cells and significantly reduce white blood cells of rabbits. (7) Antiulcer effect: radix Codonopsis has effects in resisting gastric mucosa injury and enhancing gastric mucosa cell protection. (8) Impact on immune function: the codonopsis pilosula decoction can obviously promote the biosynthesis of DNA and protein of spleen lymphocytes activated by ConA and has obvious enhancement effect on the generation of leukocyte mediator-2 (IL-2). It also has effect in enhancing phagocytic function of mouse reticuloendothelial system. (9) Fatigue resistance and anti-hypoxia effect: the codonopsis pilosula decoction can prolong the weight swimming time of the mice; and can significantly prolong the survival time of the hypoxic mouse.

Colla Corii Asini has effects of replenishing blood, stopping bleeding, nourishing yin, and moistening dryness. It is mainly indicated for syndrome of blood deficiency, consumptive disease hemoptysis, hematemesis, hematuria, hematochezia, bloody dysentery, pregnancy bleeding, metrorrhagia and metrostaxis, yin deficiency, vexation, insomnia, cough due to lung deficiency, and convulsion due to stirring of deficient wind. There are many aspects to the action of E-jiao. For example, (1) effects on the hematopoietic system: the donkey-hide gelatin has strong blood-enriching function and the curative effect is better than that of iron preparation. (2) Anti-shock effect: can restore the blood pressure of extremely low level to normal level and has long-lasting effect. (3) Effect on progressive nutritional myodegeneration: in guinea pig trials, human-like, nutritional progressive myodegeneration may occur. (4) Effects on calcium metabolism: has positive calcium balance effect, and is also beneficial to patients with muscle degeneration. The traditional Chinese medicine mainly uses the donkey-hide gelatin to treat diseases caused by blood deficiency clinically. With the improvement of the life quality of people, more and more people use the donkey-hide gelatin to build up the body and maintain beauty and keep young. (1) Treating blood diseases: the donkey-hide gelatin-containing Chinese patent medicine for treating anemia has obvious effects of treating iron deficiency anemia, aplastic anemia, thrombocytopenia, leukopenia and the like, and contains the following donkey-hide gelatin: donkey-hide gelatin blood-enriching oral liquid, Shandong donkey-hide gelatin paste, compound donkey-hide gelatin liquid, angelica blood-nourishing pills and the like. (2) And (3) fetus protection and fetus protection: colla Corii Asini can also be used for threatened abortion, and habitual abortion in gestation period. (3) Menstruation disease: donkey-hide gelatin has the main actions of nourishing yin and blood, removing blood stasis and stopping bleeding. For menstrual diseases and blood deficiency, E jiao can be used for tonifying, blood excess can be used for consolidating and controlling, and blood deficiency can be used for promoting blood circulation. (4) Bleeding: through compatibility, colla Corii Asini can be used for the treatment of various hemorrhagic diseases, such as hematemesis, hematochezia, hematuria, menorrhagia, metrorrhagia and metrostaxis and postpartum hemorrhage. (5) Preventing and treating senile diseases and delaying aging: the donkey-hide gelatin has the functions of nourishing blood, enriching blood and tonifying qi, and is used for treating various senile diseases such as climacteric syndrome, insomnia, sick sinus syndrome, hypotension, diabetes, rheumatoid arthritis, osteoporosis, senile dementia and the like. (6) And (3) cancer prevention and resistance: the expression is to improve the lymphocyte transformation rate of tumor patients, inhibit the growth of tumors, and reduce the toxicity of other anti-cancer drugs and chemotherapy, so that the treatment process can be successfully completed. The donkey-hide gelatin is commonly used in clinic in traditional Chinese medicine to be matched with other medicines for treating leukemia, nasopharyngeal carcinoma, esophageal cancer, lung cancer, breast cancer and the like. (7) Strengthening physique and beautifying: the donkey-hide gelatin is not warm and dry, is suitable for both the old and the young, can be used all the year round, and is a good tonic product for building up the body. Until now, in many families in Shanghai, Jiangsu and Zhejiang, donkey-hide gelatin is used in winter and then is matched with rock sugar, walnut kernel, black sesame, longan, Chinese date and the like to prepare thick paste which is taken with warm boiled water in the morning and evening, and people feel strong strength, enhanced cold resistance and less cold after the thick paste is taken. The donkey-hide gelatin is taken by pregnant women before and after delivery, which is beneficial to the growth of children and the recovery of the body after delivery. The donkey-hide gelatin is rich in protein degradation components, has the effect of moistening the skin by enriching the blood, is beneficial to the health care of the skin, can make the face ruddy, tender, glossy and good in elasticity after being used, has certain effect of removing speckles, and is also used for protecting and nourishing the skin. The donkey-hide gelatin is widely applied to Chinese medicine formulas and folks, and is also widely applied to the production of Chinese patent medicines. 181 of the Chinese patent medicines approved by the state in 2000 contain donkey-hide gelatin.

The molecular formula of the ferrous gluconate is C12H22O14Fe & 2H2O, and the relative molecular mass is 482.18. Can be used as color fixative and nutrition enhancer in food, and is prepared by neutralizing gluconic acid with reduced iron. The ferrous gluconate has the characteristics of high bioavailability, good solubility in water, flat flavor and no astringent taste, and is more strengthened in milk beverages. Ferrous gluconate is an excellent iron supplement, has high ferrous absorption rate, is immediately oxidized into ferric ions after entering blood, is combined with transferrin in blood plasma to form plasma iron, is transported to tissues such as liver, spleen, bone and the like by taking the transferrin as a carrier, and is stored in a ferritin form, iron of the ferritin in the bone can be combined into hemoglobin, and the hemoglobin is formed in skeletal muscle, so that the plasma iron transport rate is increased and the absorption rate is accelerated when iron is deficient, and the iron absorption amount is increased. Ferrous gluconate is excreted mainly through the intestine and skin, and a small amount through bile and sweat. Ferrous gluconate, like ferrous sulfate, participates in the synthesis of hemoglobin and plays an important role in oxygen transfer and metabolic activities of the human body. The ferrous gluconate is mainly used for treating iron deficiency anemia caused by chronic blood loss, menorrhagia, pregnant women, lactating women and children in development period.

The product formula of the invention comprehensively utilizes two types of components to achieve better effect; the product is an oral liquid preparation, has the advantages of quick absorption, quick effect, convenient use, stable quality, easy carrying, easy storage, more accurate dosage and the like, and is certainly popular with people. Therefore, the Hairikang Xueerhong oral liquid is researched and developed to meet the market demand, and has wide market prospect and far-reaching social benefit.

Anemia is a blood disease which is commonly expressed by the reduction of the total amount of cells or hemoglobin of the whole body, so that the oxygen carrying function of blood is insufficient, and the traditional Chinese medicine considers that: the generation of blood is closely related to the spleen, so "the middle energizer receives qi and takes juice to indicate blood" in the "Nei Jing" (internal classic of medicine); ling Shu (Ling Shu) refers to the treatment of deficiency of spleen and stomach, which is commonly called blood disease; the spleen-rib theory discusses that when the spleen and stomach are weakened and the function is disordered, the food is not stored enough, and the essence is deficient or the absorption and utilization of the essence are disturbed, which affects the generation of blood. Nutritional anemia is caused by malnutrition and hematopoietic disorder, and iron deficiency anemia belongs to the nutritional anemia. The primary disease is treated actively, excessive blood loss is prevented, and the disease is treated by killing insects and supplementing iron in western medicine clinically; in traditional Chinese medicine, the basic principle of benefiting qi and generating blood is adopted to synthesize symptoms, namely syndrome differentiation plus-minus, and methods of tonifying liver and kidney, warming and invigorating spleen and kidney, invigorating spleen and supplementing qi, tonifying qi and nourishing yin and the like are applied. The formula is based on the Chinese medicine theory, refers to the empirical prescription for improving anemia at all times, combines the latest progress of modern medicine research, and is prepared according to the principle of Chinese medicine theory group. The Hairikang Xueerhong oral liquid is based on the action mechanism, and aims to supplement nutrient substances necessary for hematopoiesis of human bodies and prevent the hypohematopoiesis caused by insufficient formation of hemoglobin or generation of red blood cells. The formula is the astragalus root which can greatly tonify qi of spleen and fu organs and can be used as a source for qi and blood generation; chinese angelica, sweet and pungent with warm property, nourishes blood and nourishes ying, promotes yang and yin, and promotes qi and blood; colla Corii Asini, radix rehmanniae Preparata with effects of nourishing yin and blood, and radix Codonopsis with effects of invigorating qi and nourishing blood; ferrous gluconate for preventing iron deficiency anemia.

In the formula of the composition, the astragalus has the functions of improving cardiovascular and cerebrovascular vessels, relieving the accumulation of calcium in myocardial cells, myocardial tissues and erythrocytes caused by ischemia, and protecting the calcium pump function of the erythrocytes, thereby relieving the change of the myocardial ultrastructure caused by ischemia and hypoxia. Angelica sinensis can increase the number of peripheral red blood cells, white blood cells, hemoglobin and bone marrow nucleated cells. Radix rehmanniae Preparata has effects of enhancing immunity, delaying aging, resisting tumor, tranquilizing mind, resisting radiation, resisting oxidation, affecting protein metabolism, glucocorticoid and blood sugar. Radix Codonopsis has effects of resisting oxidation, promoting increase of acetylcholine, improving memory, promoting erythropoiesis, dilating capillary vessel, and increasing blood flow. The donkey-hide gelatin has certain effect on the hematopoietic function of bone marrow, can quickly recover hemoglobin and red blood cells of hemorrhagic anemia, and has strong blood enriching effect. Ferrous gluconate has the functions of synthesizing hemoglobin, synthesizing myoglobin, synthesizing stored iron and the like; are essential components for constituting various metalloenzymes or activating certain metalloenzymes and its cofactors.

One of the most fundamental hazards of anemia to health is that the oxygen carrying capacity is low, which affects the exertion of various system functions of the whole body, resulting in a series of common symptoms and physical signs affecting the life and quality of life of people. The composition uses the donkey-hide gelatin and the prepared rehmannia root as the medicines, is sweet, warm, thick in taste, soft and moist in quality, is good at nourishing yin and blood, angelica sinensis is capable of enriching the blood and nourishing the liver, regulating blood and regulating menstruation, blood is a tangible substance, and the qi belongs to an intangible purpose, and the blood circulation depends on the qi, so that the codonopsis pilosula and the astragalus root are used for greatly tonifying the qi of spleen and lung to enrich the source of qi and blood generation. Ferrous gluconate is used as iron supplement. It is indicated for nutritional anemia. The health-care tea has a unique formula, is safe and nontoxic, has a definite health-care function and has a wide development prospect. The composition has the advantages of easily available raw materials, reasonable process, low production cost, reasonable dosage form and convenient carrying, and is suitable for middle-low income patients. Therefore, the product can be expected to have wide development prospect in the market, and will certainly generate great social benefit and good economic benefit.

In the formula of the traditional Chinese medicine composition, the astragalus membranaceus greatly tonifies qi of spleen and lung so as to provide a source for qi and blood generation; chinese angelica, sweet and pungent with warm property, nourishes blood and nourishes ying, promotes yang and yin, and promotes qi and blood; colla Corii Asini, radix rehmanniae Preparata with effects of nourishing yin and blood, and radix Codonopsis with effects of invigorating qi and replenishing blood; ferrous gluconate supplements iron. The efficacy is as follows: tonify qi and nourish blood, and nourish yin and nourish blood. The health care function is as follows: improving nutritional anemia. Radix astragali is dried root of Astragalus membranaceus bge or Astragalus membranaceus bge of Leguminosae. The radix astragali contains astragaloside, astragalus polysaccharides, choline, sucrose, flavone, amino acids, bitter principle, folic acid, flavan compound, selenium, silicon, zinc, cobalt, daucosterol, soybean saponin, etc. Nature and taste: sweet and slightly warm; spleen and lung meridian entered; the functions are as follows: invigorating qi, invigorating yang, tonifying wei-defensive qi, consolidating superficial resistance, expelling toxin, promoting granulation, inducing diuresis, and relieving swelling. Indications and application: astragalus root is the essential drug for invigorating qi, and raw astragalus root runs out of the exterior and is mainly used for strengthening the exterior, arresting sweating, expelling toxin and discharging pus; moxibustion is focused on invigorating qi, raising yang, inducing diuresis to alleviate edema. The root of Angelica sinensis (oliv.) Diels of Umbelliferae is used as the root of Angelica sinensis (oliv.) Diels. The angelica has been used as a medicine for a long time, and is listed as a traditional Chinese medicine capable of attacking and tonifying as early as Shen nong Ben Cao Jing, which can not only strengthen the body resistance and tonify the body resistance, but also attack pathogenic factors to treat diseases, and the primary effect of the angelica is to enrich the blood. The radix Angelicae sinensis contains ferulic acid, various amino acids, radix Angelicae sinensis polysaccharide, pyrimidine, purine, and vitamin B₁₂Vitamin E, linoleic acid, succinic acid, nicotinic acid, etc., and contains trace elements such as manganese, zinc, etc., and volatile oil. Nature and taste: sweet, pungent and warm; heart, liver and spleen meridians entered; the functions are as follows: tonify blood, activate blood, regulate menstruation, alleviate pain, moisten intestines and relieve constipation. Indications and application: for blood deficiency, it is combined with Huang Qi to treat blood deficiency. ② treating moonIrregular menstruation, amenorrhea and dysmenorrhea, which are the key gynecological menstruation-regulating herbs. Radix rehmanniae Preparata is root of rehmannia glutinosa Libosch which is a perennial herb of Scrophulariaceae family. Radix rehmanniae Preparata contains sitosterol, mannitol, small amount of stigmasterol, lutein, catalpol, dihydrocatalpol, acylated catalpol, daucosterol, digitoside, monoterpene and glycosides, and also contains organic acid, glucose, sucrose, fructose, raffinose, mannitol, galactose, vitamin A, multiple amino acids, and multiple trace elements. Nature and taste: sweet and slightly warm; meridian tropism: entering liver and kidney meridians; the efficacy is as follows: nourish blood and yin, supplement essence and benefit marrow. Indications and application: it is used to treat sallow complexion, dizziness, palpitation, insomnia, menoxenia, metrorrhagia, etc. ② for treating kidney yin deficiency, tidal fever, night sweat, spermatorrhea, diabetes and other symptoms. ③ all the syndromes of essence and blood deficiency, such as soreness of waist and knees, dizziness, dim eyesight, tinnitus, deafness, early white beard and hair, etc. The radix Codonopsis is dried root of Codonopsis Pilosulae of Campanulaceae. Radix Codonopsis contains stigmasterol, saponin, inulin, choline, radix Codonopsis acid, syringin, nicotine, amino acids, microelements, and vitamin B₁、B₂Volatile oil, etc. Nature and taste: sweet and mild; meridian tropism: entering spleen and lung meridians; the efficacy is as follows: tonify middle-jiao and Qi, promote the production of body fluid and harmonize stomach. Indications and application: can be used for treating qi deficiency syndrome, manifested by short breath, palpitation, asthenia, loose stool, edema, chronic diarrhea, rectocele, body fluid deficiency, dry tongue, and thirst. Anemia: is combined with codonopsis pilosula, astragalus, donkey-hide gelatin, deer horn gelatin, longan pulp, bighead atractylodes rhizome, angelica, Chinese date and the like. Leukopenia due to chemotherapy or radiotherapy: is prepared from radix Codonopsis, radix astragali, caulis Spatholobi, fructus Ligustri Lucidi, herba Epimedii, Atractylodis rhizoma, rhizoma Dioscoreae, colla Cornus Cervi, etc. The colla Corii Asini is the skin of donkey of Equidae, and is prepared by bleaching, removing hair, and decocting to obtain a block. The donkey-hide gelatin contains gelatin, bone glue, nitrogen 16.43-16.54%, protein, amino acid, calcium, sulfur, etc., lysine, arginine, histidine, etc. Nature and taste: sweet and mild; meridian tropism: entering lung, liver and kidney meridians; the functions are as follows: enrich blood and stop bleeding, nourish yin and moisten lung. Indications and application: for vertigo due to blood deficiency and palpitation, it is combined with qi-tonifying and blood-nourishing herbs, such as Dang Shen, Huang Qi, Dang Gui and Di Huang, etc. ② essential hemostatic herbs for hematemesis, epistaxis, hematochezia and metrorrhagia and metrostaxis, Pu Huang, Sheng Di Huang and ai Ye Tao charcoal can be used singly or combined. ③ replenishing blood, nourishing yin and treating yinDeficiency, vexation, insomnia, etc. Ferrous gluconate is a nutrient enhancer, and has an iron content of 11%. The iron in the body can be divided into two parts, essential and non-essential, according to its function. The essential part accounts for 70% of the total amount of iron in the body and is present in hemoglobin, myoglobin, heme enzymes, cofactors and transport iron; the non-essential part then serves as an in vivo reserve of iron, mainly in the form of ferritin and ferrihemoxanthin present in the liver, spleen and bone marrow. Iron is an essential element which is most abundant in human body, can synthesize hemoglobin, is an essential component which forms various metalloenzymes, can activate certain metalloenzymes and auxiliary factors thereof to participate in the synthesis of hormone or enhance the action of hormone, and has the functions of maintaining the normal immune function of the body and enhancing the sterilization and phagocytosis of neutrophils. Iron deficiency anemia is a global disease, and the incidence rates of the world health organization survey are 10% for adults, 40% for women and 50% for children; china reports the highest morbidity of women and children. Since many enzymes involved in human metabolism contain iron, many enzymes are reduced in activity when the human body lacks iron, causing various metabolic disorders, thereby affecting the functions of various organs. Iron is the most abundant trace element in human body, and many iron-containing enzymes control important oxidation, hydrolysis and transformation processes of the body, so that the iron-containing enzymes are related to tissue absorption, oxidative phosphorylation, porphyrin metabolism, collagen synthesis, lymphocyte and granulocyte functions, synthesis and decomposition of nerve mediators and development of body and nerve tissues. In iron deficiency anemia, hemoglobin, myoglobin, ferritin, ferrihemoglobin and the like are reduced, and simultaneously, the activity of a plurality of iron-containing enzymes or iron-dependent enzymes is reduced, so that an electron transfer system is influenced, lipid, protein and sugar metabolism is abnormal, the normal operation of body metabolism is seriously influenced, iron deficiency can cause various tissue changes and function exchange, and the content of DNA and RNA in bone marrow nucleated cells is reduced. Anemia is a blood disease which is commonly expressed by the reduction of the total amount of cells or hemoglobin of the whole body, so that the oxygen carrying function of blood is insufficient, and the traditional Chinese medicine considers that: the generation of blood is closely related to the spleen, so "the middle energizer receives qi and takes juice to indicate blood" in the "Nei Jing" (internal classic of medicine); ling Shu (Ling Shu) refers to the treatment of deficiency of spleen and stomach, which is commonly called blood disease; the spleen-Rib Lun (treatise on spleen-Rib disease) states that the spleen and stomach are impaired and dysfunctionalWhen the food is not stored enough, the food is lack of the source of the fine substances or the absorption and utilization of the fine substances are obstructed, which affects the generation of blood. Nutritional anemia is caused by malnutrition and hematopoietic disorder, and iron deficiency anemia belongs to the nutritional anemia. The primary disease is treated actively, excessive blood loss is prevented, and the disease is treated by killing insects and supplementing iron in western medicine clinically; in traditional Chinese medicine, the basic principle of benefiting qi and generating blood is adopted to synthesize symptoms, namely syndrome differentiation plus-minus, and methods of tonifying liver and kidney, warming and invigorating spleen and kidney, invigorating spleen and supplementing qi, tonifying qi and nourishing yin and the like are applied. The formula is based on the Chinese medicine theory, refers to the empirical prescription for improving anemia at all times, combines the latest progress of modern medicine research, and is prepared according to the principle of Chinese medicine theory group. The Hairikang Xueerhong oral liquid is based on the action mechanism, and aims to supplement nutrient substances necessary for hematopoiesis of human bodies and prevent the hypohematopoiesis caused by insufficient formation of hemoglobin or generation of red blood cells. The formula is the astragalus root which can greatly tonify qi of spleen and fu organs and can be used as a source for qi and blood generation; chinese angelica, sweet and pungent with warm property, nourishes blood and nourishes ying, promotes yang and yin, and promotes qi and blood; colla Corii Asini, radix rehmanniae Preparata with effects of nourishing yin and blood, and radix Codonopsis with effects of invigorating qi and nourishing blood; ferrous gluconate for preventing iron deficiency anemia.

In terms of the technical effects of the astragalus-angelicae gel oral liquid of the present invention, some experiments, such as high temperature treatment experiments, performed in the present invention can be confirmed. The effects of the astragalus, angelica and donkey-hide gelatin oral liquid obtained in the embodiment 1 of the invention on improving qi and blood functions and enhancing immunity of human bodies are proved by using the oral liquid by a large number of people. In addition, the astragalus, angelica and donkey-hide gelatin oral liquid of the invention also shows other technical effects, for example, the invention carries out the following high-temperature treatment test: the oral liquids obtained in examples 1 to 10 were left at 40 ℃ for 6 months (referred to as "high temperature 6 month treatment"), and the change in the content of the target substance from 0 month was observed at 6 months, as well as the presence or absence of the precipitate formation, according to the four types of measurement described in test example 1. The results showed that at 6 months, all the oral liquids of examples 1 to 9 had different degrees of precipitates in terms of the properties of the oral liquid, and were suspended by shaking; in contrast, all nine oral solutions of example 10 had no precipitate and remained as a clear solution. In the aspect of the change of the chemical components of the oral liquid, except calycosin glucoside, the contents of other measured objects of different oral liquids have no obvious change relative to the sample within 0 month; however, the different oral liquid samples showed different changes at 6 months, characterized by the relative percentage of calycosin glucoside at 6 months (relative to the relative percentage at 0 months, i.e. the percentage obtained by dividing the concentration at 6 months by the concentration at 0 months and multiplying by 100%), all the oral liquids of examples 1-9 ranged from 84-88%, while all the oral liquids of example 10 ranged from 98.2-99.8%, which is significantly higher than those of examples 1-9, e.g. 86.3% for example 1 and 98.7% for example 101. In a supplementary experiment, example 11, reference was made to example 10, except that sodium citrate was added only in the corresponding step, and sorbitol was not added, and after the same treatment at high temperature for 6 months, all the obtained oral liquids had precipitates of different degrees, and were suspended after shaking, and the relative percentage content of calycosin glucoside 6 months was in the range of 85 to 87. In a supplementary experiment, example 12, reference was made to example 10, except that sorbitol was added only in the corresponding steps, and sodium citrate was not added, and after the same treatment at high temperature for 6 months, all the obtained oral liquids had precipitates of different degrees, and were suspended after shaking, and the relative percentage content of calycosin glucoside 6 months was in the range of 86-89%. In a supplementary test, example 13, reference is made to example 10, except that sodium citrate and sorbitol are added before the first decoction in step (1), the obtained oral liquid is treated at the same high temperature for 6 months, and then all the oral liquid have precipitates of different degrees, and are in a suspension state after shaking, and the relative percentage content of calycosin glucoside for 6 months is 87-90%. The other measurement targets of each oral liquid of examples 11 to 13 were not significantly different before and after the treatment at high temperature for 6 months. In addition, the two typical oral liquids obtained in example 1 and example 101 of the present invention have substantially the same therapeutic effects in terms of the functions of improving qi and blood functions and enhancing immunity of a human body clinically, and have significant therapeutic effects in terms of improving qi and blood functions and enhancing immunity of a human body. For example, two oral liquids of example 1 and example 101 were used for 120 and 90 anemic subjects, respectively, and were completely cured.

The oral solution prepared by combining the astragalus, the angelica, the prepared rehmannia root, the codonopsis pilosula, the donkey-hide gelatin and the ferrous gluconate not only can effectively improve the qi and blood functions of a human body, but also can improve the immunity.

Detailed Description

The present invention will be further described by the following examples, however, the scope of the present invention is not limited to the following examples. It will be understood by those skilled in the art that various changes and modifications may be made to the invention without departing from the spirit and scope of the invention. The present invention has been described generally and/or specifically with respect to materials used in testing and testing methods. Although many materials and methods of operation are known in the art for the purpose of carrying out the invention, the invention is nevertheless described herein in as detail as possible.

In the following examples, when preparing the composition, if not specifically mentioned, the amount of astragalus per administration is not less than 1200g, the prepared oral liquid is finally packaged in glass bottles, 10ml per bottle; however, when the formulation is described below, it is expressed in terms of the amount of the Chinese medicinal composition to be administered per 100ml of the oral liquid to be prepared.

In the invention, the prepared traditional Chinese medicine composition in the form of oral liquid can be called Xueerhong oral liquid; or the oral liquid can be called as Hairikang brand oral liquid of astragalus, angelica and donkey-hide gelatin because the oral liquid comprises three important medicinal materials of astragalus, angelica and donkey-hide gelatin.

Example 1: preparation of traditional Chinese medicine composition of astragalus, angelica and donkey-hide gelatin oral liquid

The formula is as follows: 12g of astragalus, 5g of angelica, 4g of prepared rehmannia root, 4g of codonopsis pilosula, 2g of donkey-hide gelatin, 0.32g of ferrous gluconate, 12g of white granulated sugar and a proper amount of purified water are prepared into 100 mL.

The preparation method comprises the following steps:

(1) decocting radix astragali, radix Angelicae sinensis, radix rehmanniae Preparata, and radix Codonopsis with purified water twice (6 times of purified water for 2 hr for the first time, filtering to obtain filtrate, decocting the residue with 6 times of purified water for 2 hr for the second time, filtering to obtain filtrate), and mixing the filtrates;

(2) concentrating the combined filtrate under reduced pressure until the weight ratio of the liquid medicine to the medicinal materials is 1: 1; adding 95% ethanol to make alcohol content reach 60%, standing, precipitating with ethanol for 24 hr, collecting supernatant, and filtering to obtain filtrate;

(3) recovering ethanol from the filtrate obtained in the step (2) at 70 ℃ under reduced pressure until no alcohol smell exists, and concentrating the alcohol-free liquid under reduced pressure until the relative density is 1.05-1.08 (the measurement temperature is 40 ℃), so as to obtain a traditional Chinese medicine extracting solution for later use;

(4) smashing colla Corii Asini into small pieces, adding 10 times of purified water, heating to dissolve completely, and filtering;

(5) mixing ferrous gluconate and flavoring agent, adding 3 times of purified water, and heating for dissolving;

(6) mixing the above medicinal liquids of steps (3), (4) and (5), mixing, adding purified water to desired volume, stirring, filtering, packaging, sealing, sterilizing under heat and pressure (105 deg.C for 45min), and making into oral liquid.

Example 2: preparation of traditional Chinese medicine composition of astragalus, angelica and donkey-hide gelatin oral liquid

The formula is as follows: 12g of astragalus, 4.5g of angelica, 4.5g of prepared rehmannia root, 3.5g of codonopsis pilosula, 2.5g of donkey-hide gelatin, 0.25g of ferrous gluconate, 14g of white granulated sugar and a proper amount of purified water are prepared into 100 mL.

The preparation method comprises the following steps:

(1) decocting radix astragali, radix Angelicae sinensis, radix rehmanniae Preparata, and radix Codonopsis with purified water twice (5 times of purified water for 3 hr for the first time, filtering to obtain filtrate, decocting the residue with 8 times of purified water for 2 hr for the second time, filtering to obtain filtrate), and mixing the filtrates;

(2) concentrating the combined filtrate under reduced pressure until the weight ratio of the liquid medicine to the medicinal materials is 0.8: 1; adding 98% ethanol to make ethanol content reach 55%, standing, precipitating with ethanol for 20 hr, collecting supernatant, and filtering to obtain filtrate;

(3) recovering ethanol from the filtrate obtained in the step (2) at 80 ℃ under reduced pressure until no alcohol smell exists, and concentrating the alcohol-free liquid under reduced pressure until the relative density is 1.05-1.08 (the measurement temperature is 40 ℃) to obtain a traditional Chinese medicine extracting solution for later use;

(4) smashing colla Corii Asini into small pieces, adding 8 times of purified water, heating to dissolve completely, and filtering;

(5) mixing ferrous gluconate and flavoring agent, adding 4 times of purified water, and heating for dissolving;

(6) mixing the above medicinal liquids of steps (3), (4) and (5), mixing, adding purified water to desired volume, stirring, filtering, packaging, sealing, sterilizing under heat and pressure (105 deg.C for 45min), and making into oral liquid.

Example 3: preparation of traditional Chinese medicine composition of astragalus, angelica and donkey-hide gelatin oral liquid

The formula is as follows: 12g of astragalus, 5.5g of angelica, 3.5g of prepared rehmannia root, 4.5g of codonopsis pilosula, 1.5g of donkey-hide gelatin, 0.35g of ferrous gluconate, 10g of white granulated sugar and a proper amount of purified water are prepared into 100 mL.

The preparation method comprises the following steps:

(1) decocting radix astragali, radix Angelicae sinensis, radix rehmanniae Preparata, and radix Codonopsis with purified water twice (8 times of purified water for 1 hr for the first time, filtering to obtain filtrate, decocting the residue with 5 times of purified water for 3 hr for the second time, filtering to obtain filtrate), and mixing the filtrates;

(2) concentrating the combined filtrate under reduced pressure until the weight ratio of the liquid medicine to the medicinal materials is 1.2: 1; adding 95% ethanol to make ethanol content reach 65%, standing for precipitating with ethanol for 30 hr, collecting supernatant, and filtering to obtain filtrate;

(3) recovering ethanol from the filtrate obtained in the step (2) at 60 ℃ under reduced pressure until no alcohol smell exists, and concentrating the alcohol-free liquid under reduced pressure until the relative density is 1.05-1.08 (the measurement temperature is 40 ℃), so as to obtain a traditional Chinese medicine extracting solution for later use;

(4) smashing colla Corii Asini into small pieces, adding 12 times of purified water, heating to dissolve completely, and filtering;

(5) mixing ferrous gluconate and flavoring agent, adding 2 times of purified water, and heating for dissolving;

(6) mixing the above medicinal liquids of steps (3), (4) and (5), mixing, adding purified water to desired volume, stirring, filtering, packaging, sealing, sterilizing under heat and pressure (105 deg.C for 45min), and making into oral liquid.

Example 4: preparation of traditional Chinese medicine composition of astragalus, angelica and donkey-hide gelatin oral liquid

The formula is as follows: 12g of astragalus, 6g of angelica, 3g of prepared rehmannia root, 5g of codonopsis pilosula, 1g of donkey-hide gelatin, 0.2g of ferrous gluconate, 16g of white granulated sugar and a proper amount of purified water are prepared into 100 mL.

The preparation method comprises the following steps:

(1) decocting radix astragali, radix Angelicae sinensis, radix rehmanniae Preparata, and radix Codonopsis with purified water twice (7 times of purified water for 2 hr for the first time, filtering to obtain filtrate, decocting residue with 7 times of purified water for 1 hr for the second time, filtering to obtain filtrate), and mixing the filtrates;

(2) concentrating the combined filtrate under reduced pressure until the weight ratio of the liquid medicine to the medicinal materials is 0.9: 1; adding 90% ethanol to make ethanol content reach 65%, standing for 25 hr for precipitating with ethanol, collecting supernatant, and filtering to obtain filtrate;

(3) recovering ethanol from the filtrate obtained in the step (2) at 75 ℃ under reduced pressure until no alcohol smell exists, and concentrating the alcohol-free liquid under reduced pressure until the relative density is 1.05-1.08 (the measurement temperature is 40 ℃), so as to obtain a traditional Chinese medicine extracting solution for later use;

(4) smashing colla Corii Asini into small pieces, adding 9 times of purified water, heating to dissolve completely, and filtering;

(5) mixing ferrous gluconate and flavoring agent, adding 2.5 times of purified water, and heating for dissolving;

(6) mixing the above medicinal liquids of steps (3), (4) and (5), mixing, adding purified water to desired volume, stirring, filtering, packaging, sealing, sterilizing under heat and pressure (105 deg.C for 45min), and making into oral liquid.

Example 5: preparation of traditional Chinese medicine composition of astragalus, angelica and donkey-hide gelatin oral liquid

The formula is as follows: 12g of astragalus, 4g of angelica, 5g of prepared rehmannia root, 3g of codonopsis pilosula, 3g of donkey-hide gelatin, 0.4g of ferrous gluconate, 8g of white granulated sugar and a proper amount of purified water are prepared into 100 mL.

The preparation method comprises the following steps:

(1) decocting radix astragali, radix Angelicae sinensis, radix rehmanniae Preparata, and radix Codonopsis with purified water twice (6 times of purified water for 2 hr for the first time, filtering to obtain filtrate, decocting the second residue with 5 times of purified water for 2 hr, filtering to obtain filtrate), and mixing the filtrates;

(2) concentrating the combined filtrate under reduced pressure until the weight ratio of the liquid medicine to the medicinal materials is 1.1: 1; adding 96% ethanol to make alcohol content reach 62%, standing, precipitating with ethanol for 28 hr, collecting supernatant, and filtering to obtain filtrate;

(3) recovering ethanol from the filtrate obtained in the step (2) at 65 ℃ under reduced pressure until no alcohol smell exists, and concentrating the alcohol-free liquid under reduced pressure until the relative density is 1.05-1.08 (the measurement temperature is 40 ℃) to obtain a traditional Chinese medicine extracting solution for later use;

(4) smashing colla Corii Asini into small pieces, adding 11 times of purified water, heating to dissolve completely, and filtering;

(5) mixing ferrous gluconate and flavoring agent, adding 3.5 times of purified water, and heating for dissolving;

(6) mixing the above medicinal liquids of steps (3), (4) and (5), mixing, adding purified water to desired volume, stirring, filtering, packaging, sealing, sterilizing under heat and pressure (105 deg.C for 45min), and making into oral liquid.

Example 6: preparation of traditional Chinese medicine composition of astragalus, angelica and donkey-hide gelatin oral liquid

The formula is as follows: 12g of astragalus, 5g of angelica, 4g of prepared rehmannia root, 4g of codonopsis pilosula, 2g of donkey-hide gelatin, 0.32g of ferrous gluconate, 12g of white granulated sugar and a proper amount of purified water are prepared into 100 mL.

The preparation method comprises the following steps:

(1) decocting radix astragali, radix Angelicae sinensis, radix rehmanniae Preparata, and radix Codonopsis with purified water twice (6.5 times of purified water for 2.5 hr for the first time, filtering to obtain filtrate, decocting residue with 7.5 times of purified water for 1.5 times, filtering to obtain filtrate), and mixing the filtrates;

(2) concentrating the combined filtrate under reduced pressure until the weight ratio of the liquid medicine to the medicinal materials is 1: 1; adding 97% ethanol to make ethanol content reach 60%, standing, precipitating with ethanol for 26 hr, collecting supernatant, and filtering to obtain filtrate;

(3) recovering ethanol from the filtrate obtained in the step (2) at 70 ℃ under reduced pressure until no alcohol smell exists, and concentrating the alcohol-free liquid under reduced pressure until the relative density is 1.05-1.08 (the measurement temperature is 40 ℃), so as to obtain a traditional Chinese medicine extracting solution for later use;

(4) smashing colla Corii Asini into small pieces, adding 11 times of purified water, heating to dissolve completely, and filtering;

(5) mixing ferrous gluconate and flavoring agent, adding 4 times of purified water, and heating for dissolving;

(6) mixing the above medicinal liquids of steps (3), (4) and (5), mixing, adding purified water to desired volume, stirring, filtering, packaging, sealing, sterilizing under heat and pressure (105 deg.C for 45min), and making into oral liquid.

Example 7: preparation of traditional Chinese medicine composition of astragalus, angelica and donkey-hide gelatin oral liquid

The formula is as follows: 12g of astragalus, 5g of angelica, 4g of prepared rehmannia root, 4g of codonopsis pilosula, 2g of donkey-hide gelatin, 0.32g of ferrous gluconate, 12g of white granulated sugar and a proper amount of purified water are prepared into 100 mL.

The preparation method comprises the following steps:

(1) decocting radix astragali, radix Angelicae sinensis, radix rehmanniae Preparata, and radix Codonopsis with purified water twice (7 times of purified water for 1 hr for the first time, filtering to obtain filtrate, decocting residue with 6.5 times of purified water for 1.5 hr for the second time, filtering to obtain filtrate), and mixing the filtrates;

(2) concentrating the combined filtrate under reduced pressure until the weight ratio of the liquid medicine to the medicinal materials is 0.9: 1; adding 98% ethanol to make ethanol content reach 65%, standing, precipitating with ethanol for 24 hr, collecting supernatant, and filtering to obtain filtrate;

(3) recovering ethanol from the filtrate obtained in the step (2) at 75 ℃ under reduced pressure until no alcohol smell exists, and concentrating the alcohol-free liquid under reduced pressure until the relative density is 1.05-1.08 (the measurement temperature is 40 ℃), so as to obtain a traditional Chinese medicine extracting solution for later use;

(4) smashing colla Corii Asini into small pieces, adding 8 times of purified water, heating to dissolve completely, and filtering;

(5) mixing ferrous gluconate and flavoring agent, adding 4 times of purified water, and heating for dissolving;

(6) mixing the above medicinal liquids of steps (3), (4) and (5), mixing, adding purified water to desired volume, stirring, filtering, packaging, sealing, sterilizing under heat and pressure (105 deg.C for 45min), and making into oral liquid.

Example 8: preparation of traditional Chinese medicine composition of astragalus, angelica and donkey-hide gelatin oral liquid

The formula is as follows: 12g of astragalus, 5g of angelica, 4g of prepared rehmannia root, 4g of codonopsis pilosula, 2g of donkey-hide gelatin, 0.32g of ferrous gluconate, 12g of white granulated sugar and a proper amount of purified water are prepared into 100 mL.

The preparation method comprises the following steps:

(1) decocting radix astragali, radix Angelicae sinensis, radix rehmanniae Preparata, and radix Codonopsis with purified water twice (5 times of purified water for 3 hr for the first time, filtering to obtain filtrate, decocting residue with 6 times of purified water for 2 hr for the second time, filtering to obtain filtrate), and mixing the filtrates;

(2) concentrating the combined filtrate under reduced pressure until the weight ratio of the liquid medicine to the medicinal materials is 0.9: 1; adding 95% ethanol to make the alcohol content reach 60%, standing and precipitating with ethanol for 20-30 hours, taking supernatant, and filtering to obtain filtrate;

(3) recovering ethanol from the filtrate obtained in the step (2) at 76 ℃ under reduced pressure until no alcohol smell exists, and concentrating the alcohol-free liquid under reduced pressure until the relative density is 1.05-1.08 (the measurement temperature is 40 ℃) to obtain a traditional Chinese medicine extracting solution for later use;

(4) smashing colla Corii Asini into small pieces, adding 11 times of purified water, heating to dissolve completely, and filtering;

(5) mixing ferrous gluconate and flavoring agent, adding 3 times of purified water, and heating for dissolving;

(6) mixing the above medicinal liquids of steps (3), (4) and (5), mixing, adding purified water to desired volume, stirring, filtering, packaging, sealing, sterilizing under heat and pressure (105 deg.C for 45min), and making into oral liquid.

Example 9: preparation of traditional Chinese medicine composition of astragalus, angelica and donkey-hide gelatin oral liquid

The formula is as follows: 12g of astragalus, 5g of angelica, 4g of prepared rehmannia root, 4g of codonopsis pilosula, 2g of donkey-hide gelatin, 0.32g of ferrous gluconate, 12g of white granulated sugar and a proper amount of purified water are prepared into 100 mL.

The preparation method comprises the following steps:

(1) decocting radix astragali, radix Angelicae sinensis, radix rehmanniae Preparata, and radix Codonopsis with purified water twice (7 times of purified water for 2 hr for the first time, filtering to obtain filtrate, decocting the second residue with 8 times of purified water for 1.5 hr, filtering to obtain filtrate), and mixing the filtrates;

(2) concentrating the combined filtrate under reduced pressure until the weight ratio of the liquid medicine to the medicinal materials is 1: 1; adding 95% ethanol to make alcohol content reach 60%, standing, precipitating with ethanol for 20 hr, collecting supernatant, and filtering to obtain filtrate;

(3) recovering ethanol from the filtrate obtained in the step (2) at a temperature of 68 ℃ under reduced pressure until no alcohol smell exists, and concentrating the alcohol-free liquid under reduced pressure until the relative density is 1.05-1.08 (the measurement temperature is 40 ℃), so as to obtain a traditional Chinese medicine extracting solution for later use;

(4) smashing colla Corii Asini into small pieces, adding 9 times of purified water, heating to dissolve completely, and filtering;

(5) mixing ferrous gluconate and flavoring agent, adding 2 times of purified water, and heating for dissolving;

(6) mixing the above medicinal liquids of steps (3), (4) and (5), mixing, adding purified water to desired volume, stirring, filtering, packaging, sealing, sterilizing under heat and pressure (105 deg.C for 45min), and making into oral liquid.

Example 10: preparation of traditional Chinese medicine composition of astragalus, angelica and donkey-hide gelatin oral liquid

Referring to the formulation and preparation method of examples 1 to 9, respectively, except that sodium citrate (the weight ratio of astragalus root and sodium citrate is 12: 0.1) and sorbitol (the weight ratio of astragalus root and sorbitol is 12: 0.5) are further added to the filtrate obtained in step (2), and then the reduced pressure recovery of ethanol and concentration operation of step (4) are performed to obtain 10 batches of oral liquid, which can be referred to as samples of example 101 and example 102 … …, respectively, in sequence, as example 109.

Test example 1: the oral liquid obtained in each of the above embodiments 1 to 10 is measured for typical component content according to a method of corresponding medicinal material varieties in the first phase of the "chinese pharmacopoeia" 2015 edition, and the measurement target includes: astragalus (determination of astragaloside IV and calycosin glucoside), prepared rehmannia root (determination of verbascoside), angelica (determination of ferulic acid), and codonopsis pilosula (determination of codonopsis pilosula alkynoside by RP-HPLC method, wherein the determination method comprises the steps of Rong-run and the like, analysis of content of codonopsis pilosula alkynoside in Shanxi codonopsis pilosula medicinal materials, journal of drug analysis, 2007 stage 08). The results show that 9 batches of oral liquids obtained in example 10 are the same as the corresponding batches of oral liquids in examples 1 to 9, and no difference is found, for example, the oral liquid in example 1 and the oral liquid in example 101 have no difference in the content of the four types of measurement targets. In addition, the oral liquid obtained in each of examples 1 to 10 was a clear solution after preparation, and no precipitate was observed.

Claims (12)

1. A Chinese medicinal composition is prepared from (by weight parts) radix astragali 1200, radix Angelicae sinensis 400-600, radix rehmanniae Preparata 300-500, radix Codonopsis 300-500, colla Corii Asini 100-300, ferrous gluconate 20-40, and medicinal adjuvants by making into oral liquid; the traditional Chinese medicine composition is prepared by the method comprising the following steps:

(1) decocting radix astragali, radix Angelicae sinensis, radix rehmanniae Preparata, and radix Codonopsis with purified water twice, and mixing the filtrates;

(2) concentrating the combined filtrate under reduced pressure until the weight ratio of the liquid medicine to the medicinal materials is 0.8-1.2: 1; adding ethanol to enable the alcohol content to reach 55-65%, standing and precipitating with ethanol for 20-30 hours, taking supernate, filtering to obtain filtrate, adding sodium citrate and sorbitol into the filtrate, wherein the weight ratio of the medicinal materials of radix astragali to sodium citrate is 12: 0.1, the weight ratio of the medicinal materials of astragalus and sorbitol is 12: 0.5;

(3) recovering ethanol from the filtrate obtained in the step (2) at the temperature of 60-80 ℃ under reduced pressure until no alcohol smell exists, concentrating the alcohol-free liquid under reduced pressure until the relative density is 1.05-1.08, and measuring the temperature to 40 ℃ to obtain a traditional Chinese medicine extracting solution for later use;

(4) smashing donkey-hide gelatin into small pieces, adding 8-12 times of purified water, heating to completely dissolve the donkey-hide gelatin, and filtering for later use;

(5) mixing ferrous gluconate and an optional flavoring agent, and adding 2-4 times of purified water for heating and dissolving for later use;

(6) mixing the above medicinal liquids of steps (3), (4) and (5), mixing, adding purified water to desired volume, stirring, filtering, packaging, sealing, and sterilizing under hot pressure.

2. The Chinese medicinal composition according to claim 1, which is prepared from 1200 parts by weight of astragalus membranaceus, 450-550 parts by weight of angelica sinensis, 350-450 parts by weight of prepared rehmannia root, 350-450 parts by weight of codonopsis pilosula, 150-250 parts by weight of donkey-hide gelatin, 25-35 parts by weight of ferrous gluconate and a pharmaceutical excipient.

3. The Chinese medicinal composition according to claim 1, which is prepared from 1200 parts by weight of astragalus membranaceus, 500 parts by weight of angelica sinensis, 400 parts by weight of prepared rehmannia root, 400 parts by weight of codonopsis pilosula, 200 parts by weight of donkey-hide gelatin, 32 parts by weight of ferrous gluconate and a pharmaceutical excipient.

4. The traditional Chinese medicine composition according to claim 1, which is prepared by using 8-16 g of astragalus root medicine per 100 ml.

5. The traditional Chinese medicine composition according to claim 1, which is prepared by using 10-14 g of astragalus root medicine per 100 ml.

6. The Chinese medicinal composition according to claim 1, which is prepared by using 12g of astragalus root per 100 ml.

7. The traditional Chinese medicine composition according to claim 1, wherein said flavoring agent is selected from the group consisting of aspartame, acesulfame potassium, saccharin sodium, sucrose.

8. The traditional Chinese medicine composition according to claim 1, wherein in the step (1), the astragalus, the angelica, the prepared rehmannia root and the codonopsis pilosula are taken, are added with purified water for decoction twice, 5-8 times of the purified water is added for decoction for 1-3 hours for the first time, and are filtered to obtain filtrate; and adding 5-8 times of purified water into the second filter residue, decocting for 1-3 hours, filtering to obtain filtrate, and combining the two filtrates.

9. The Chinese medicinal composition according to claim 1, wherein in the step (2), the ethanol is ethanol with a concentration of 90% or more.

10. The Chinese medicinal composition according to claim 1, wherein in the step (6), the autoclaving is sterilization at 105 ℃ for 45 min.

11. A process for preparing a Chinese medicinal composition as claimed in any one of claims 1 to 10, comprising the steps of:

(1) decocting radix astragali, radix Angelicae sinensis, radix rehmanniae Preparata, and radix Codonopsis with purified water twice, and mixing the filtrates;

(2) concentrating the combined filtrate under reduced pressure until the weight ratio of the liquid medicine to the medicinal materials is 0.8-1.2: 1; adding ethanol to enable the alcohol content to reach 55-65%, standing and precipitating with ethanol for 20-30 hours, taking supernate, filtering to obtain filtrate, adding sodium citrate and sorbitol into the filtrate, wherein the weight ratio of the medicinal materials of radix astragali to sodium citrate is 12: 0.1, the weight ratio of the medicinal materials of astragalus and sorbitol is 12: 0.5;

(3) recovering ethanol from the filtrate obtained in the step (2) at the temperature of 60-80 ℃ under reduced pressure until no alcohol smell exists, concentrating the alcohol-free liquid under reduced pressure until the relative density is 1.05-1.08, and measuring the temperature to 40 ℃ to obtain a traditional Chinese medicine extracting solution for later use;

(4) smashing donkey-hide gelatin into small pieces, adding 8-12 times of purified water, heating to completely dissolve the donkey-hide gelatin, and filtering for later use;

(5) mixing ferrous gluconate and an optional flavoring agent, and adding 2-4 times of purified water for heating and dissolving for later use;

(6) mixing the above medicinal liquids of steps (3), (4) and (5), mixing, adding purified water to desired volume, stirring, filtering, packaging, sealing, and sterilizing under hot pressure.

12. Use of the Chinese medicinal composition of any one of claims 1 to 10 or the Chinese medicinal composition prepared by the method of claim 11 in preparing a product for improving qi and blood functions and enhancing immunity of a human body.