CN107927492A - A kind of probiotics composite beverage and preparation method thereof - Google Patents
A kind of probiotics composite beverage and preparation method thereof Download PDFInfo
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- CN107927492A CN107927492A CN201710980685.4A CN201710980685A CN107927492A CN 107927492 A CN107927492 A CN 107927492A CN 201710980685 A CN201710980685 A CN 201710980685A CN 107927492 A CN107927492 A CN 107927492A
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- 239000006041 probiotic Substances 0.000 title claims abstract description 33
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 33
- 235000013361 beverage Nutrition 0.000 title claims abstract description 32
- 239000002131 composite material Substances 0.000 title claims abstract description 26
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- UQZIYBXSHAGNOE-USOSMYMVSA-N Stachyose Natural products O(C[C@H]1[C@@H](O)[C@H](O)[C@H](O)[C@@H](O[C@@]2(CO)[C@H](O)[C@@H](O)[C@@H](CO)O2)O1)[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@H](CO[C@@H]2[C@@H](O)[C@@H](O)[C@@H](O)[C@H](CO)O2)O1 UQZIYBXSHAGNOE-USOSMYMVSA-N 0.000 claims abstract description 27
- UQZIYBXSHAGNOE-XNSRJBNMSA-N stachyose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO[C@@H]3[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O3)O)O2)O)O1 UQZIYBXSHAGNOE-XNSRJBNMSA-N 0.000 claims abstract description 27
- 235000013325 dietary fiber Nutrition 0.000 claims abstract description 17
- ZTOKUMPYMPKCFX-CZNUEWPDSA-N (E)-17-[(2R,3R,4S,5S,6R)-6-(acetyloxymethyl)-3-[(2S,3R,4S,5S,6R)-6-(acetyloxymethyl)-3,4,5-trihydroxyoxan-2-yl]oxy-4,5-dihydroxyoxan-2-yl]oxyoctadec-9-enoic acid Chemical compound OC(=O)CCCCCCC/C=C/CCCCCCC(C)O[C@@H]1O[C@H](COC(C)=O)[C@@H](O)[C@H](O)[C@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](COC(C)=O)O1 ZTOKUMPYMPKCFX-CZNUEWPDSA-N 0.000 claims abstract description 10
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- 241000186000 Bifidobacterium Species 0.000 claims description 7
- 239000012043 crude product Substances 0.000 claims description 7
- 235000017048 Garcinia mangostana Nutrition 0.000 claims description 6
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- 238000001914 filtration Methods 0.000 claims description 6
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- 238000003756 stirring Methods 0.000 claims description 6
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- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 4
- 239000000047 product Substances 0.000 claims description 4
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical group CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 3
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 3
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- 238000000227 grinding Methods 0.000 claims description 3
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- 235000014655 lactic acid Nutrition 0.000 claims description 3
- 239000002932 luster Substances 0.000 claims description 3
- 239000012466 permeate Substances 0.000 claims description 3
- 235000010413 sodium alginate Nutrition 0.000 claims description 3
- 239000000661 sodium alginate Substances 0.000 claims description 3
- 229940005550 sodium alginate Drugs 0.000 claims description 3
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical group [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 claims description 3
- 229940078499 tricalcium phosphate Drugs 0.000 claims description 3
- 229910000391 tricalcium phosphate Inorganic materials 0.000 claims description 3
- 235000019731 tricalcium phosphate Nutrition 0.000 claims description 3
- 238000002137 ultrasound extraction Methods 0.000 claims description 3
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 3
- 229920001285 xanthan gum Polymers 0.000 claims description 3
- 108091005804 Peptidases Proteins 0.000 claims description 2
- 239000004365 Protease Substances 0.000 claims description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 2
- 125000003071 maltose group Chemical group 0.000 claims description 2
- 241001409321 Siraitia grosvenorii Species 0.000 claims 1
- 238000012869 ethanol precipitation Methods 0.000 claims 1
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- BHQCQFFYRZLCQQ-OELDTZBJSA-N cholic acid Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 BHQCQFFYRZLCQQ-OELDTZBJSA-N 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 244000185386 Thladiantha grosvenorii Species 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 4
- 229940099352 cholate Drugs 0.000 description 4
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- 102000004190 Enzymes Human genes 0.000 description 2
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- 235000011389 fruit/vegetable juice Nutrition 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- HYBBIBNJHNGZAN-UHFFFAOYSA-N furfural Chemical compound O=CC1=CC=CO1 HYBBIBNJHNGZAN-UHFFFAOYSA-N 0.000 description 2
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- BHQCQFFYRZLCQQ-UHFFFAOYSA-N (3alpha,5alpha,7alpha,12alpha)-3,7,12-trihydroxy-cholan-24-oic acid Natural products OC1CC2CC(O)CCC2(C)C2C1C1CCC(C(CCC(O)=O)C)C1(C)C(O)C2 BHQCQFFYRZLCQQ-UHFFFAOYSA-N 0.000 description 1
- 241000186046 Actinomyces Species 0.000 description 1
- 239000004380 Cholic acid Substances 0.000 description 1
- 241000207199 Citrus Species 0.000 description 1
- 241000193171 Clostridium butyricum Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 229930186217 Glycolipid Natural products 0.000 description 1
- 208000031226 Hyperlipidaemia Diseases 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000235342 Saccharomycetes Species 0.000 description 1
- HIWPGCMGAMJNRG-ACCAVRKYSA-N Sophorose Natural products O([C@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O)[C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HIWPGCMGAMJNRG-ACCAVRKYSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
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- 238000009825 accumulation Methods 0.000 description 1
- 210000000577 adipose tissue Anatomy 0.000 description 1
- HIWPGCMGAMJNRG-UHFFFAOYSA-N beta-sophorose Natural products OC1C(O)C(CO)OC(O)C1OC1C(O)C(O)C(O)C(CO)O1 HIWPGCMGAMJNRG-UHFFFAOYSA-N 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 235000019416 cholic acid Nutrition 0.000 description 1
- 229960002471 cholic acid Drugs 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000005238 degreasing Methods 0.000 description 1
- KXGVEGMKQFWNSR-UHFFFAOYSA-N deoxycholic acid Natural products C1CC2CC(O)CCC2(C)C2C1C1CCC(C(CCC(O)=O)C)C1(C)C(O)C2 KXGVEGMKQFWNSR-UHFFFAOYSA-N 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
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- 239000003814 drug Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 238000002376 fluorescence recovery after photobleaching Methods 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 235000012631 food intake Nutrition 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
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- 230000004060 metabolic process Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
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- 230000000529 probiotic effect Effects 0.000 description 1
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- PZDOWFGHCNHPQD-VNNZMYODSA-N sophorose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](C=O)O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O PZDOWFGHCNHPQD-VNNZMYODSA-N 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
- A23L2/60—Sweeteners
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
- A23L33/22—Comminuted fibrous parts of plants, e.g. bagasse or pulp
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
The present invention provides a kind of probiotics composite beverage, it is made of the component of following parts by weight:12 15 parts of probiotics, 20 25 parts of stachyose, 10 13 parts of water-soluble dietary fiber, 8 11 parts of sweetener, 12 parts of stabilizer, 23 parts of sophorolipid, 1 1.5 parts of thickener, 0.5 1 parts of anticaking agent.Present invention also offers the preparation method of the probiotics composite beverage.Probiotics composite beverage provided by the present invention not only can effectively prevent diarrhea, constipation, also have preferable anti-oxidant, norcholesterol performance.
Description
Technical field
The present invention relates to a kind of probiotics composite beverage and preparation method thereof.
Background technology
Due to the quickening of modern life rhythm, people also face family and life in addition to bearing huge operating pressure
Pressure in work, so frequently results in " Neuro-endocrine-immunity " autonomous regulatory function imbalance of human body, and food intake is excessive
Or the change of organism metabolism causes body fat accumulation, excess weight to increase, and cause human pathology, physiological change.Overfeeding
The disease such as hyperlipidemia and human body intestinal canal lesion also become the master for influencing health of people gradually caused by sweet fertilizer, pure wine do not save etc.
Want factor.
With the development of science and technology, the research that Food Science combines together with preventive medicine, makes food have special health care
Function becomes the important development direction of food scientific technology, while people constantly occur to change to the demand of nutrition.Collection science and technology,
Fashion, health, nutrition in one probiotic beverage thus increasingly welcome be subject to consumer, probiotics be one kind to host
Beneficial active microorganism, is to be colonized in human body intestinal canal, in reproductive system, and it is micro- so as to improve host to produce definite health efficacy
The ecological balance, the active beneficial microorganism general name for playing beneficial effect.Beneficial bacterium or fungi are main in human body, animal body
Have:Clostridium butyricum, lactobacillus, Bifidobacterium, actinomyces, saccharomycete etc..
Chinese patent application CN201510209023.8 discloses a kind of preparation side of pawpaw soya-bean milk probiotics composite beverage
Method, (1) first Chinese flowering quince juice and soybean milk processed, Chinese flowering quince juice is allocated in proportion with soybean milk, the mistake after 50-60 DEG C completely enzymolysis
Filter obtains pawpaw soya-bean milk;(2) sucrose, milk powder, emulsifying agent and thickener are added into pawpaw soya-bean milk and the compound drink of pawpaw soya-bean milk is made
Material;(3) probiotics for accounting for pawpaw soya-bean milk composite beverage quality 2-5% is added into pawpaw soya-bean milk composite beverage again, at 40-45 DEG C
Lower culture 2-4h, then in 0~4 DEG C of after-ripening 10-15h, that is, obtains product.Although the invention unique flavor, nutritive value are higher,
But function is relatively simple.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of probiotics composite beverage, its not only can effectively prevent diarrhea,
Constipation, also has preferable anti-oxidant, norcholesterol performance.
In order to solve the above technical problems, the technical scheme is that:
A kind of probiotics composite beverage, it is made of the component of following parts by weight:12-15 parts of probiotics, stachyose 20-
25 parts, 10-13 parts of water-soluble dietary fiber, 8-11 parts of sweetener, 1-2 parts of stabilizer, 2-3 parts of sophorolipid, thickener 1-1.5
Part, 0.5-1 parts of anticaking agent.
Further, the preparation process of the stachyose is:
1) dried after Siraitia grosvenorii is cleaned at 60 DEG C, obtain mangosteen powder after being crushed with pulverizer, mangosteen powder is added
In the water of 30 times of weight, extracting solution is obtained by filtration after being extracted 50 minutes at 65 DEG C, with activity after being fermented with lactic acid bacteria to extracting solution
Carbon decoloring, obtains stachyose crude product after filtering;
2) doughnut that stachyose crude product is 3kD with molecular cut off is subjected to the hyperfiltration treatment that pressure is 0.02MPa,
Stachyose will be obtained after obtained permeate vacuum freeze drying to constant weight.
Further, the preparation process of the water-soluble dietary fiber is:
1) epidermis of fresh convex top mandarin orange is peeled, be placed in baking oven at 100 DEG C dry 2 it is small when, be transferred to the ether of 4 times of weight
In, when immersion 4 is small at room temperature, air-dried after taking-up, 80 mesh sieves are crossed after grinding and obtain convex top orange peel powder;
2) convex top orange peel powder is added in the deionized water of 15 times of weight, it is 5.0 that pH value is adjusted after being heated to 45 DEG C, uses egg
Ultrasonic extraction 30 minutes at 45 DEG C, obtain filtrate, filtrate are sunk with 95% ethanol of 4 times of volumes after white enzyme enzymolysis after centrifugation
Form sediment 2 it is small when, obtain filter residue after centrifugation, water-soluble dietary fiber obtained after being freeze-dried to constant weight.
Further, the probiotics is Bifidobacterium.
Further, the sweetener is maltose.
Further, the stabilizer is sodium alginate.
Further, the thickener is xanthans.
Further, the anticaking agent is tricalcium phosphate.
Another technical problem to be solved by the present invention is that provide the preparation method of above-mentioned probiotics composite beverage.
In order to solve the above technical problems, technical solution is:
A kind of preparation method of probiotics composite beverage, comprises the following steps:
It is sent into after choosing each component in clean area, each component is crossed into 80 mesh sieves respectively, weighs each group respectively in parts by weight
Point, be sent into while stirring in mixer, stir and obtain mixed powder untill the homogeneous no color differnece of color and luster, mixed powder is carried out in
Bag packing, progress outer encapsulating fills to obtain probiotics composite beverage finished product after producing clean area.
Compared with prior art, the invention has the advantages that:
1) main component of probiotics is Bifidobacterium in the present invention, it prevents diarrhea, constipation performance with stronger, because
This present invention can effectively prevent diarrhea, constipation.
2) Siraitia grosvenorii is the fruit of the perennial liana of Curcurbitaceae, and the present invention is carried by water after Siraitia grosvenorii is cleaned crushing
Method obtains extracting solution, then obtains stachyose crude product with lactobacillus-fermented rear decoloring, and stachyose purifying crude then is obtained purity
Higher stachyose, it is a kind of multi-functional oligosaccharide, can effectively reduce cholesterol, therefore the present invention has preferable drop courage
Sterol performance, in addition, stachyose of the present invention also can effectively prevent constipation, and produces synergistic effect with Bifidobacterium, so that
Further improve the avoid constipation performance of the present invention.
3) convex top mandarin orange is Rutaceae citrus plant, and the present invention with ether carries out degreasing pretreatment after its epidermis is peeled,
Convex top orange peel powder is obtained after being ground up, sieved, then it is used to ultrasonic wave added protease hydrolyzed method by the water solubility in convex top mandarin orange epidermis
Dietary fiber effectively extracts, which has higher antioxidation activity, can greatly improve the present invention's
Antioxygenic property, and it also has effects that norcholesterol, synergistic effect can be produced with stachyose, so as to further improve this
The norcholesterol performance of invention.
4) sophorolipid is a kind of Glycolipids Biosurfactantss, can effectively improve the dispersiveness of each component of the present invention, from
And further improve every excellent properties of the present invention;In addition, sophorolipid also has stronger cold tolerance, therefore can also be effective
Improve the cold resistance of the present invention.
Embodiment
Below in conjunction with specific embodiment, the present invention will be described in detail, herein schematic description and description of the invention
For explaining the present invention, but it is not as a limitation of the invention.
Embodiment 1-4
Probiotics composite beverage is prepared according to 1 ratio of table (in terms of parts by weight):
Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | |
Bifidobacterium | 12 | 15 | 13 | 14 |
Stachyose | 25 | 20 | 23 | 21 |
Water-soluble dietary fiber | 13 | 10 | 11 | 12 |
Maltose | 11 | 8 | 9 | 10 |
Sodium alginate | 2 | 1 | 1.5 | 1.8 |
Sophorolipid | 3 | 2 | 2.5 | 2.8 |
Xanthans | 1.5 | 1 | 1.4 | 1.3 |
Tricalcium phosphate | 1 | 0.5 | 0.6 | 0.8 |
Table 1
The preparation method of embodiment 1-4 comprises the following steps:
1) dried after Siraitia grosvenorii is cleaned at 60 DEG C, obtain mangosteen powder after being crushed with pulverizer, mangosteen powder is added
In the water of 30 times of weight, extracting solution is obtained by filtration after being extracted 50 minutes at 65 DEG C, with activity after being fermented with lactic acid bacteria to extracting solution
Carbon decoloring, obtains stachyose crude product after filtering;
2) doughnut that stachyose crude product is 3kD with molecular cut off is subjected to the hyperfiltration treatment that pressure is 0.02MPa,
Stachyose will be obtained after obtained permeate vacuum freeze drying to constant weight;
3) epidermis of fresh convex top mandarin orange is peeled, be placed in baking oven at 100 DEG C dry 2 it is small when, be transferred to the ether of 4 times of weight
In, when immersion 4 is small at room temperature, air-dried after taking-up, 80 mesh sieves are crossed after grinding and obtain convex top orange peel powder;
4) convex top orange peel powder is added in the deionized water of 15 times of weight, it is 5.0 that pH value is adjusted after being heated to 45 DEG C, uses egg
Ultrasonic extraction 30 minutes at 45 DEG C, obtain filtrate, filtrate are sunk with 95% ethanol of 4 times of volumes after white enzyme enzymolysis after centrifugation
Form sediment 2 it is small when, obtain filter residue after centrifugation, water-soluble dietary fiber obtained after being freeze-dried to constant weight;
5) it is sent into after choosing each component in clean area, each component is crossed into 80 mesh sieves respectively, is weighed respectively in parts by weight each
Component, is sent into mixer, stirs and obtain mixed powder untill the homogeneous no color differnece of color and luster, mixed powder is carried out while stirring
Interior bag packing, progress outer encapsulating fills to obtain probiotics composite beverage finished product after producing clean area.
Comparative Examples 1
Do not include stachyose in component, other components and preparation method are same as Example 4.
Comparative Examples 2
Do not include water-soluble dietary fiber in component, other components and preparation method are same as Example 4.
Comparative Examples 3
Do not include sophorolipid in component, other components and preparation method are same as Example 4.
Comparative example
Comparative example is the embodiment 1 of the Chinese patent of Application No. CN201510209023.8.
Experimental example one:Avoid constipation performance test
Test method is:The constipation patient that 100 times of defecation in one week are less than 3 times is chosen, drinks each beverage 1 daily
It is secondary, the defecation frequency in patient time week is counted after 1 week, calculates avoid constipation effective percentage, efficient=secondary all defecation frequencies of avoid constipation
Number/total number of persons × 100% more than or equal to 5 times, test result are as shown in table 2:
Table 2
As seen from Table 2, the avoid constipation effective percentage of 1-4 of the embodiment of the present invention is far above comparative example, shows tool of the present invention
There is preferable avoid constipation performance, the avoid constipation performance of wherein embodiment 4 is best.The constituent part of Comparative Examples 1-3 and implementation
Example 4 is different, and the avoid constipation effective percentage of wherein Comparative Examples 1 has and reduces by a small margin, illustrates that stachyose can effectively improve avoid constipation
Performance;The avoid constipation of Comparative Examples 2 is efficient to maintain an equal level with embodiment 1-4, illustrates water-soluble dietary fiber to avoid constipation performance
Substantially do not influence;The efficient range of decrease of avoid constipation of Comparative Examples 3 is more slightly larger than Comparative Examples 1, illustrates that sophorolipid can have
Effect improves Bifidobacterium, the dispersiveness of stachyose.
Experimental example two:Antioxygenic property is tested
The total antioxidant capacity of each beverage is measured by FRAP methods, total antioxidant capacity is higher to show anti-oxidation efficacy more
Good, test result is as shown in table 3:
Total antioxidant capacity (mmolTrolox/100mL) | |
Embodiment 1 | 0.1377 |
Embodiment 2 | 0.1370 |
Embodiment 3 | 0.1360 |
Embodiment 4 | 0.1388 |
Comparative Examples 1 | 0.1374 |
Comparative Examples 2 | 0.0099 |
Comparative Examples 3 | 0.1212 |
Comparative example | 0.0095 |
Table 3
As seen from Table 3, the total antioxidant capacity of 1-4 of the embodiment of the present invention is far above comparative example, shows tool of the present invention
There is preferable antioxygenic property, the antioxygenic property of wherein embodiment 4 is best.The constituent part of Comparative Examples 1-3 and implementation
Example 4 is different, and wherein the total antioxidant capacity of Comparative Examples 1 maintains an equal level with embodiment 1-4, illustrates stachyose to antioxygenic property base
This is not influenced;The total antioxidant capacity of Comparative Examples 2 declines to a great extent, and it is to greatly improve antioxygen to illustrate water-soluble dietary fiber
Change the key factor of performance;The total antioxidant capacity of Comparative Examples 3 has to be declined by a small margin, illustrates that sophorolipid can effectively improve water
The dispersiveness of soluble dietary fiber.
Experimental example three:Norcholesterol performance test
The cholate solution that 3.2mL concentration is 2g/L is measured, is separately added into each beverages of 5mg, water-bath insulation 1 is small at 37 DEG C
When, centrifuged 8 minutes with the speed of 5000rpm after cooled to room temperature, supernatant absorption 2mL is referred into furfural development process
Absorbance is measured, the concentration of cholate in supernatant is calculated according to standard curve, calculates inhibiting rate:Inhibiting rate=(cholate
Cholic acid salinity in initial concentration solution-supernatant)/cholate solution initial concentration × 100%.Inhibiting rate is higher to be shown to drop courage
Sterol performance is better, and test result is as shown in table 4:
Inhibiting rate (%) | |
Embodiment 1 | 42.37 |
Embodiment 2 | 42.16 |
Embodiment 3 | 42.22 |
Embodiment 4 | 42.44 |
Comparative Examples 1 | 40.45 |
Comparative Examples 2 | 40.59 |
Comparative Examples 3 | 37.48 |
Comparative example | 0.22 |
Table 4
As seen from Table 4, the inhibiting rate of 1-4 of the embodiment of the present invention is far above comparative example, and it is preferable to show that the present invention has
Norcholesterol performance, the norcholesterol performance of wherein embodiment 4 is best.The constituent part of Comparative Examples 1-3 and embodiment 4
Difference, the inhibiting rate of wherein Comparative Examples 1,2 has to be declined by a small margin, illustrates that stachyose and water-soluble dietary fiber can have
Effect improves norcholesterol performance;The fall of the inhibiting rate of Comparative Examples 3 is slightly larger than Comparative Examples 1,2, illustrates sophorose
Fat can effectively improve the dispersiveness of stachyose and water-soluble dietary fiber.
Experimental example four:Cold resistance is tested
Each beverage is placed in 0 DEG C of freezer, is taken out after 20 days and observe the caking situation of beverage, test result such as table
Shown in 5:
Table 5
As seen from Table 5,1-4 of the embodiment of the present invention does not occur caking situation, and it is preferable cold-resistant to show that the present invention has
Property.The constituent part of Comparative Examples 1-3 is different from embodiment 4, and wherein Comparative Examples 1,2 do not occur caking situation, explanation
Stachyose, water-soluble dietary fiber do not influence cold resistance;It is tight that caking situation then occur as comparative example in Comparative Examples 3
The phenomenon of weight, it is the key factor for improving cold resistance to illustrate sophorolipid.
The above-described embodiments merely illustrate the principles and effects of the present invention, not for the limitation present invention.It is any ripe
Know the personage of this technology all can carry out modifications and changes under the spirit and scope without prejudice to the present invention to above-described embodiment.Cause
This, those of ordinary skill in the art is complete without departing from disclosed spirit and institute under technological thought such as
Into all equivalent modifications or change, should by the present invention claim be covered.
Claims (9)
- A kind of 1. probiotics composite beverage, it is characterised in that:It is made of the component of following parts by weight:12-15 parts of probiotics, 20-25 parts of stachyose, 10-13 parts of water-soluble dietary fiber, 8-11 parts of sweetener, 1-2 parts of stabilizer, 2-3 parts of sophorolipid, thickening 1-1.5 parts of agent, 0.5-1 parts of anticaking agent.
- A kind of 2. probiotics composite beverage according to claim 1, it is characterised in that:The preparation process of the stachyose For:1) dried after Siraitia grosvenorii is cleaned at 60 DEG C, mangosteen powder is obtained after being crushed with pulverizer, mangosteen powder is added 30 times In the water of weight, extracting solution is obtained by filtration after being extracted 50 minutes at 65 DEG C, is taken off after being fermented with lactic acid bacteria to extracting solution with activated carbon Color, obtains stachyose crude product after filtering;2) doughnut that stachyose crude product is 3kD with molecular cut off is subjected to the hyperfiltration treatment that pressure is 0.02MPa, will To permeate vacuum freeze drying to constant weight after obtain stachyose.
- A kind of 3. probiotics composite beverage according to claim 2, it is characterised in that:The system of the water-soluble dietary fiber It is for step:1) epidermis of fresh convex top mandarin orange is peeled, be placed in baking oven at 100 DEG C dry 2 it is small when, be transferred in the ether of 4 times of weight, When immersion 4 is small at room temperature, air-dried after taking-up, 80 mesh sieves are crossed after grinding and obtain convex top orange peel powder;2) convex top orange peel powder is added in the deionized water of 15 times of weight, it is 5.0 that pH value is adjusted after being heated to 45 DEG C, uses protease Ultrasonic extraction 30 minutes at 45 DEG C, obtain filtrate after enzymolysis after centrifugation, 95% ethanol precipitation 2 by filtrate with 4 times of volumes Hour, filter residue is obtained after centrifugation, water-soluble dietary fiber is obtained after being freeze-dried to constant weight.
- A kind of 4. probiotics composite beverage according to claim 3, it is characterised in that:The probiotics is Bifidobacterium.
- A kind of 5. probiotics composite beverage according to claim 4, it is characterised in that:The sweetener is maltose.
- A kind of 6. probiotics composite beverage according to claim 5, it is characterised in that:The stabilizer is sodium alginate.
- A kind of 7. probiotics composite beverage according to claim 6, it is characterised in that:The thickener is xanthans.
- A kind of 8. probiotics composite beverage according to claim 7, it is characterised in that:The anticaking agent is tricalcium phosphate.
- A kind of 9. preparation method of probiotics composite beverage according to claim 1~8 any one, it is characterised in that: Comprise the following steps:It is sent into after choosing each component in clean area, each component is crossed into 80 mesh sieves respectively, weighs each component, side respectively in parts by weight Stir side to be sent into mixer, stir and obtain mixed powder untill the homogeneous no color differnece of color and luster, mixed powder is wrapped inside point Dress, progress outer encapsulating fills to obtain probiotics composite beverage finished product after producing clean area.
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Publication number | Priority date | Publication date | Assignee | Title |
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