CN107927416A - A kind of chicken feed addictive and its chicken feed for purifying salmonella - Google Patents
A kind of chicken feed addictive and its chicken feed for purifying salmonella Download PDFInfo
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- CN107927416A CN107927416A CN201711141479.0A CN201711141479A CN107927416A CN 107927416 A CN107927416 A CN 107927416A CN 201711141479 A CN201711141479 A CN 201711141479A CN 107927416 A CN107927416 A CN 107927416A
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- salmonella
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
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- Polymers & Plastics (AREA)
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- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Birds (AREA)
- Feed For Specific Animals (AREA)
Abstract
The present invention provides a kind of chicken feed addictive and its chicken feed for purifying salmonella.The chicken feed addictive of the purification salmonella, includes each component of following percentage by weight：Organic acid 5~50%；C8~C24 aliphatic acid 5~50%；Terpenes 5~30%；Trans 2 hexenoic aldehyde 5~30%；And inert carrier 5~50%.The chicken feed addictive of above-mentioned purification salmonella, due to equipped with terpenes and C8~C24 aliphatic acid, after multiple material mixings with antibacterial effect, produce synergistic effect, the chicken feed addictive of above-mentioned purification salmonella can effectively kill the salmonella with chicken feed or chicken, have the advantages that it is safe, have no drug resistance, can effective substitute antibiotics, meet healthy aquaculture developing direction.The chicken feed addictive of this purification salmonella can promote growth of animal, improves feed efficiency and reduce disease incident.
Description
Technical field
The present invention relates to chicken feed field, more particularly to a kind of chicken feed addictive for purifying salmonella and its chicken
Feed.
Background technology
Salmonella (SALMONELLA) is a kind of common important pathogen Pseudomonas of enterobacteria, it is known that salmonella
Serum has 2499, widely distributed in nature, almost all of serotype all can contaminated food so that morbidity.The bacterium is dirty
After contaminating feed, not only cause animal poisoning, cause bacteremia, performance vomiting, diarrhea, abdominal pain simultaneously cause local inflammation or exacerbation
Local inflammation, and cause the propagation of salmonella to circulate i.e. feed-animal-raw-food material-people, economic loss is both caused, more
It is important that influencing human health, " livestock product safety " engineering for currently going all out to build to us constitutes serious prestige
The side of body.The infection chain points out that feedstuff and finished product feedstuff are the preludes for triggering infection, ultimately results in the salmonella infection of people,
It is important zoonosis pathogeny.Countries in the world government makes very the limitation of salmonella in livestock products at present
Stringent standard, is most stringent of in all microorganism limit standards.
Antibiotic was once widely applied as growth promoter (Antimicrobial growthpromoter, APG)
Husbandry sector process, improves husbandry sector efficiency；But largely cause to resist using antibiotic in production and human treatment's process
The generation of pharmacological property pathogenic strain, may inactivate antibiotic during human treatment's disease.Multiple drug resistance salmonella is sent out
It is existing, therefore be continuing with APG and be colonized in Broiler Chicken as control salmonella that more resistance to the action of a drug Salmonellas will likely be caused
Bacterium.European Union forbade applications of the APG in husbandry sector from 2006, and the U.S. is also advocating antibiotic and is being only used as treating
Means use.All detect that a variety of Foodborne salmonellas are positive in domestic multiple regional meat products and poultry body, main bacterial strain
There are Bacterium enteritidis, salmonella typhimurium, S. pullonum etc., and detect that tetracycline, acidum nalidixicum, chlorine are mould
Element, the higher resistant rate of ampicillin.Therefore, APG substitutes are found to be of great significance.
The content of the invention
Based on this, to solve the above problems, it is necessary to provide a kind of chicken feed addictive for purifying salmonella and its chicken
Feed.
A kind of chicken feed addictive for purifying salmonella, includes each component of following percentage by weight：
Above-mentioned technical proposal, since equipped with terpenes and C8~C24 aliphatic acid, multiple materials with antibacterial effect being mixed
After conjunction, the effect of synergy is produced, the chicken feed addictive of above-mentioned purification salmonella can effectively kill chicken feed or chicken
Salmonella with it, have the advantages that it is safe, have no drug resistance, can effective substitute antibiotics, meet healthy aquaculture hair
Open up direction.The chicken feed addictive of this purification salmonella can promote growth of animal, improves feed efficiency and reduce disease
Incidence.
In one of the embodiments, the emulsifying agent that percentage by weight is 1~10% is further included.
In one of the embodiments, the trans -2- hexenoic aldehydes that percentage by weight is 5~30% are further included.
Above-mentioned technical proposal, due to that equipped with the trans -2- hexenoic aldehydes with antibacterial activity, can further improve above-mentioned
The bactericidal effect of the chicken feed addictive of salmonella is purified, further reduces disease incident.
In one of the embodiments, the emulsifying agent is nonionic surfactant, ionic surface active agent or both sexes
Surfactant.
In one of the embodiments, the nonionic surfactant is the ethoxy that hydrophilic lipophilic balance is 4~18
Base castor oil surfactant.
In one of the embodiments, it is characterised in that the organic acid is formic acid, propionic acid, fumaric acid, lactic acid and second
One or more in acid.
In one of the embodiments, the aliphatic acid for octanoic acid, n-nonanoic acid, certain herbaceous plants with big flowers acid, laurate, palmitic acid, butanedioic acid,
One or more in myristic acid.
In one of the embodiments, the terpenes is in diallyl disulfide, carvacrol, citral, eugenol, hundred
One or more in fragrant phenol, limonene and carvol.
One breeding hen feed, includes the chicken feed addictive of purification salmonella, and the chicken feed of the purification salmonella adds
It is 0.1~0.3wt% to add ratio of the agent in the chicken feed.
Above-mentioned technical proposal, due to being equipped with the chicken feed addictive of purification salmonella in chicken feed, can eat chicken
During with chicken feed, growth of animal can be promoted, improve feed efficiency and reduce disease incident.
Beneficial effect：The present invention has the following advantages：
The chicken feed addictive of above-mentioned purification salmonella, due to equipped with terpenes and C8~C24 aliphatic acid, by multiple tools
After having the material mixing of antibacterial effect, the effect of synergy is produced, the chicken feed addictive of above-mentioned purification salmonella can
Effectively kill the salmonella with chicken feed or chicken, have the advantages that it is safe, have no drug resistance, can effectively substitute anti-
Raw element, meets healthy aquaculture developing direction.The chicken feed addictive of this purification salmonella can promote growth of animal, improve and raise
Expect transformation efficiency and reduce disease incident.
Embodiment
Presently preferred embodiments of the present invention is described in detail below so that advantages and features of the invention can be easier to by
It will be appreciated by those skilled in the art that so as to make a clearer definition of the protection scope of the present invention.
Embodiment 1
A kind of chicken feed addictive for purifying salmonella, includes each component of following percentage by weight：Thymol
5.25%th, eugenol 5.25%, ethoxy aluminium castor oil CO60 5.00%, n-nonanoic acid 8%, acetic acid 26.5%, lactic acid 20%
And propionic acid 30%.
Embodiment 2
A kind of chicken feed addictive for purifying salmonella, includes each component of following percentage by weight：Thymol
5.25%th, eugenol 5.25%, ethoxy aluminium castor oil CO60 5.00%, n-nonanoic acid 8%, acetic acid 36.5%, propionic acid 30%
And trans -2- hexenoic aldehydes 10%.
Embodiment 3
A kind of chicken feed addictive for purifying salmonella, includes each component of following percentage by weight：Thymol
5.25%th, eugenol 5.25%, ethoxy aluminium castor oil CO60 5.00%, n-nonanoic acid 8%, acetic acid 26.5%, propionic acid 30%
And trans -2- hexenoic aldehydes 20%.
Embodiment 4
A kind of chicken feed addictive for purifying salmonella, includes each component of following percentage by weight：Thymol
5.25%th, eugenol 5.25%, ethoxy aluminium castor oil CO60 5.00%, n-nonanoic acid 8%, acetic acid 26.5%, propionic acid 30%
And levulic acid 20%.
Embodiment 5
A kind of chicken feed addictive for purifying salmonella, includes each component of following percentage by weight：Thymol
5.25%th, eugenol 5.25%, ethoxy aluminium castor oil CO60 5.00%, n-nonanoic acid 8%, octanoic acid 10%, acetic acid 26.5%,
Propionic acid 30% and formic acid 10%.
Embodiment 6
A kind of chicken feed addictive for purifying salmonella, includes each component of following percentage by weight：Thymol
5.25%th, eugenol 5.25%, ethoxy aluminium castor oil CO60 5.00%, n-nonanoic acid 8%, octanoic acid 10%, lactic acid 10%, third
Acid 46.5% and butanedioic acid 10%.
Embodiment 7
A kind of chicken feed addictive for purifying salmonella, includes each component of following percentage by weight：Thymol
5.25%th, eugenol 5.25%, CO60 5.00%, n-nonanoic acid 8%, acetic acid 26.5%, lactic acid 20% and propionic acid 30%.
Embodiment 8
A kind of chicken feed addictive for purifying salmonella, includes each component of following percentage by weight：Thymol
5.25%th, eugenol 5.25%, CO60 5.00%, n-nonanoic acid 8%, acetic acid 30% and propionic acid 46.5%.
Embodiment 9
A kind of chicken feed addictive for purifying salmonella, includes each component of following percentage by weight：Thymol
5.25%th, eugenol 5.25%, CO60 5.00%, n-nonanoic acid 8%, propionic acid 50% and butanedioic acid 26.5%.
Embodiment 10
A kind of chicken feed addictive for purifying salmonella, includes each component of following percentage by weight：Propionic acid 10%,
Formic acid 70% and levulic acid 20%.
Performance test
1st, the chicken feed addictive of each embodiment tests the rejection of salmonella.
The chicken feed addictive of embodiment 1~10 is added to the examination of the salmonella containing 5ml according to the ratio of 0.05wt%
Guan Zhong, test tube cultivate 24h at room temperature after being vortexed, and after bacterium solution is diluted according to different extension rates, are put down using brilliant green agar
Plate is counted, and calculates the clump count of salmonella, the results are shown in Table 1.
The chicken feed addictive of 1 embodiment 1~10 of table contrasts the inhibiting rate of salmonella
Wherein, control group 1 is the test tube of only 5ml salmonellas, and test tube cultivates 24h at room temperature after being vortexed, by bacterium solution
After different extension rate dilutions, counted using brilliant green agar tablet, calculate the clump count of salmonella.
As shown in Table 1, embodiment 1~10 all has salmonella obvious inhibitory action, illustrates that the purification of the present invention is husky
The chicken feed addictive of door Salmonella is preferable to the inhibition of salmonella.
2nd, the chicken feed addictive of each embodiment purification salmonella surveys the rejection of salmonella in chicken feed
Examination.
In salmonella-polluted chicken feed raw material meat meal tankage is subject to, be respectively 0kg/t, 1kg/t according to addition,
2kg/t, 3kg/t standard, add the chicken feed addition of the purification salmonella of embodiment 1~3, embodiment 7~8 and embodiment 9
Agent, after cultivating 24h at room temperature, takes 10g samples to be dissolved in 90mlPBS phosphate buffers, afterwards by different dilutions respectively
After multiple dilution, counted using brilliant green agar tablet, calculate the clump count of salmonella, the results are shown in Table 2.
The chicken feed addictive that table 2 respectively purifies salmonella contrasts the inhibiting rate of salmonella in your feed
As shown in Table 2, each embodiment, which has salmonella-polluted chicken feed raw material, suppresses the work that salmonella increases
With, and embodiment 3 and embodiment 10 are relative to the better of the suppression salmonella of other embodiment.
3rd, salmonella recall rate contrast test in each organ of broiler chicken that different chicken feeds are fed.
Choose 216 broiler chicken and be randomly divided into 4 treatment groups, be respectively that control group 2 (is fed with the normal chicken feed of antibiotic-free
Raising broiler), I group of experiment (press 1kg/t additive amounts, by the chicken feed addictive of embodiment 3 added to feeding in normal chicken feed
Broiler chicken), II group of experiment (press 2kg/t additive amounts, by the chicken feed addictive of embodiment 3 added to feeding meat in normal chicken feed
Chicken), III group of experiment (press 3kg/t additive amounts, by the chicken feed addictive of embodiment 3 added to feeding meat in normal chicken feed
Chicken), each treatment group is 54 broiler chicken.After each treatment group broiler chicken is fed 5 days with different chicken feeds, 27 broiler chicken in control group 2
As negative control group, 200 μ l PBS (phosphate buffer) of every broiler chicken administered by oral gavage of the negative control group are used as control,
Other 27 broiler chicken are as positive controls, III group of the positive controls, I group of experiment, II group of experiment and experiment in control group 2
Broiler chicken all 200 μ l Bacterium enteritidis of administered by oral gavage infect, and just common different chicken feeds are fed after infection, the 5th natural gift after infection
Not carry out sample collection analysis, the results are shown in Table 3.
Salmonella recall rate contrast table in each organ of 3 broiler chicken of table
| Crop | Caecum | Liver | Spleen | |
| Control group | 100 | 78 | 51 | 45 |
| Test I group | 41 | 23 | 5 | 2 |
| Test II group | 12 | 0 | 0 | 0 |
| Test III group | 5 | 0 | 0 | 0 |
As shown in Table 3, the 5th day after infection, compared with positive controls, III group of I group of experiment, II group of experiment and experiment meat
The recall rate of each organ salmonella of chicken is below positive controls, illustrates that the chicken feed addictive of the present invention can be effectively net
Change the Salmonella bacterial load in broiler chicken, have the function that to suppress salmonella.
4th, the performance in layers contrast that different chicken feeds are fed.
600 50 week old health laying hens are randomly divided into four groups by the laying rate principle of indifference, every group of four repetitions,
Each repeat 15 laying hens.Four groups are respectively control group 3 (feeding laying hen with the normal chicken feed of antibiotic-free), V group of experiment
(pressing 1kg/t additive amounts, the chicken feed addictive of embodiment 3 is added in normal chicken feed and feeds laying hen), tests VI group
(pressing 2kg/t additive amounts, the chicken feed addictive of embodiment 3 is added in normal chicken feed and feeds laying hen), VII group of experiment
(pressing 3kg/t additive amounts, the chicken feed addictive of embodiment 3 is added in normal chicken feed and feeds broiler chicken).Before on-test
3 days, laying hen water is taken into big mycin to remove the interference of original salmonella daily, then experiment every laying hen oral cavity of the same day connects
The Bacterium enteritidis 1ml that kind content is 1.3 × 108CFU, challenge test phase are 13 days, and the production performance of each group laying hen is such as
Shown in table 4, in addition, the salmonella positive rate test result such as institute of table 5 of each organ of laying hen each organ when testing the 14th day
Show.In experiment the 7th day and the 14th day morning, each 7 laying hens were butchered in group processing, the tubal assessment fallopian tubal sand of each laying hen is taken
Door Salmonella positive rate, while each group collects the 10th~13 day each 7 pieces of egg, detects egg salmonella positive rate, detection knot
Fruit is as shown in table 6.
4 each group performance in layers of table contrasts
As shown in Table 4, the production performance of each group laying hen is without significant difference, but test group performance is generally better than control
Group 3.
5 each group laying hen of table tests salmonella positive rate in the 14th day each organ and contrasts
As shown in Table 5, in test in the 14th day of experiment, 2 chickens of only control group 3 detected salmonella, its
Remaining test group does not detect salmonella, illustrates the chicken feed addictive of the present invention and can effectively suppress the increasing of salmonella
It is long.
Salmonella positive rate in 6 laying hen fallopian tubal of table and egg
As shown in Table 6, the 7th day and the 14th day after salmonella attacks poison, compared with control group 3,3 test groups drop
Low laying hen fallopian tubal salmonella positive rate, wherein testing VII group reaches 100% suppression salmonella, illustrates the present invention's
Chicken feed addictive can effectively suppress salmonella, greatly reduce the incidence of disease.
Embodiment described above only expresses the several embodiments of the present invention, its description is more specific and detailed, but simultaneously
Therefore the limitation to the scope of the claims of the present invention cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the present invention's
Protection domain.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (9)
1. a kind of chicken feed addictive for purifying salmonella, it is characterised in that include each component of following percentage by weight：
2. a kind of chicken feed addictive for purifying salmonella according to claim 1, it is characterised in that further include weight
Percentage is 1~10% emulsifying agent.
3. a kind of chicken feed addictive for purifying salmonella according to claim 1, it is characterised in that further include weight
Percentage is 5~30% trans -2- hexenoic aldehydes.
A kind of 4. chicken feed addictive for purifying salmonella according to claim 2, it is characterised in that the emulsifying agent
For nonionic surfactant, ionic surface active agent or amphoteric surfactant.
A kind of 5. chicken feed addictive for purifying salmonella according to claim 4, it is characterised in that the nonionic
Surfactant is the ethoxy castor oil surfactant that hydrophilic lipophilic balance is 4~18.
6. a kind of chicken feed addictive of purification salmonella according to Claims 1 to 5 any one, its feature exist
In the organic acid is the one or more in formic acid, propionic acid, fumaric acid, lactic acid and acetic acid.
7. a kind of chicken feed addictive of purification salmonella according to Claims 1 to 5 any one, its feature exist
In the aliphatic acid is octanoic acid, the one or more in n-nonanoic acid, certain herbaceous plants with big flowers acid, laurate, palmitic acid, butanedioic acid, myristic acid.
8. a kind of chicken feed addictive of purification salmonella according to Claims 1 to 5 any one, its feature exist
In the terpenes is one in diallyl disulfide, carvacrol, citral, eugenol, Thymol, limonene and carvol
Kind is a variety of.
A 9. breeding hen feed, it is characterised in that the chicken including the purification salmonella described in claim 1~8 any one is raised
Feed additives, ratio of the chicken feed addictive for purifying salmonella in the chicken feed is 0.1~0.3wt%.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711141479.0A CN107927416A (en) | 2017-11-15 | 2017-11-15 | A kind of chicken feed addictive and its chicken feed for purifying salmonella |
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|---|---|---|---|
| CN201711141479.0A CN107927416A (en) | 2017-11-15 | 2017-11-15 | A kind of chicken feed addictive and its chicken feed for purifying salmonella |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109170174A (en) * | 2018-10-18 | 2019-01-11 | 安吉福寿农业开发有限公司 | A kind of chicken feed addictive and its application, chicken feed |
| CN110973380A (en) * | 2019-12-27 | 2020-04-10 | 广州美瑞泰科生物工程技术有限公司 | Feed additive for preventing calf diarrhea and preparation method and application thereof |
| WO2021001578A1 (en) * | 2019-07-04 | 2021-01-07 | Nutreco Ip Assets B.V. | Feed additive |
| WO2021069572A1 (en) | 2019-10-08 | 2021-04-15 | Taminco Bvba | Antimicrobial composition for animal feed |
| WO2022217031A1 (en) * | 2021-04-08 | 2022-10-13 | Eastman Chemical Company | Methods of using formaldehyde-free antimicrobial compositions in animal by-product compositions |
| CN115812854A (en) * | 2022-05-07 | 2023-03-21 | 中国农业大学 | Feed additive and application thereof |
| CN116650452A (en) * | 2023-06-08 | 2023-08-29 | 吉林大学 | Application of citral in preparation of salmonella pilus inhibitor |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109170174A (en) * | 2018-10-18 | 2019-01-11 | 安吉福寿农业开发有限公司 | A kind of chicken feed addictive and its application, chicken feed |
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| WO2022217031A1 (en) * | 2021-04-08 | 2022-10-13 | Eastman Chemical Company | Methods of using formaldehyde-free antimicrobial compositions in animal by-product compositions |
| CN115812854A (en) * | 2022-05-07 | 2023-03-21 | 中国农业大学 | Feed additive and application thereof |
| CN116650452A (en) * | 2023-06-08 | 2023-08-29 | 吉林大学 | Application of citral in preparation of salmonella pilus inhibitor |
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