CN107904138A - The adipose tissue preparation facilities of the component containing vascular stroma is separated from adipose tissue - Google Patents
The adipose tissue preparation facilities of the component containing vascular stroma is separated from adipose tissue Download PDFInfo
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- 238000002360 preparation method Methods 0.000 title claims abstract description 19
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- 238000004140 cleaning Methods 0.000 claims abstract description 53
- 238000000926 separation method Methods 0.000 claims abstract description 51
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
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- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/28—Constructional details, e.g. recesses, hinges disposable or single use
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- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C12M45/00—Means for pre-treatment of biological substances
- C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
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Abstract
A kind of adipose tissue preparation facilities that the component containing vascular stroma is separated from adipose tissue, described device includes cleaning vibration separation cylindrical vessel, the cleaning vibration separation cylindrical vessel both ends are sealed with an inlet cap and outlet end cap respectively, the center location of the inlet cap is provided with entrance calparine cap, the center location of the outlet end cap is provided with outlet calparine cap, a tissue agglomerate cutting net is separately installed with the position of the inlet cap and the outlet end cap in the cleaning vibration separation cylindrical vessel, cut in the tissue agglomerate in the space of net and the cleaning vibration separation cylindrical vessel composition and be placed with auxiliary oscillating stainless shot.Survival rate of the present invention is high, avoids transplant infection, and the material of injection receptor site fat stem cell is prepared for physics.
Description
Technical field
The preparation facilities of the vascular stroma component of fat stem cell is included the present invention relates to a kind of separation from adipose tissue,
Particularly extract and separate from human body own fat and include fat stem cell tissue for treat osteoarthritis and cartilage damage
Vascular stroma component preparation facilities
Background technology
Osteoarthritis is a kind of degenerative joint disease for seriously affecting articular cartilage and surrounding tissue, its symptom is main
Show as that arthralgia is stiff, enlargement is powerless and action obstacle, the pain and function in lesion joint, which are damaged, seriously affects patient
Daily action life.Traditional expectant treatment mainly includes analgestic use, joint cavity injection and Chondroprotective agents, seriously
When need to by arthroscope carry out operative treatment, to patient with severe symptoms it is contemplated that carry out joint replacement surgery.For current osteoarthritis
Current treatment status, fat stem cell provide a kind for the treatment of method simple and easy to do, wound is small, curative effect is good.Research is found from fat
Vascular stroma component (stromal vascular fraction, SVF) is isolated in tissue and includes fat stem cell, it has
It is divided into the potential of cartilaginous tissue, and cartilaginous tissue is as vesselless tissue, the self-repairing capability after impaired is limited, therefore
Fat stem cell is needed to Chondrocyte Differentiation, to play the purpose in repair deficiency joint.In preliminary in vitro experiments research,
Diekman etc. successfully utilizes bone morphogenetic protein, fiber mother cell growth factor, upper table skin growth factor and conversion growth
The combined induction fat stem cell such as factor-beta is divided into cartilage cell.In addition, growth and differentiation factor is added in the medium, it is such as raw
Long differentiation factor and BMP-14 etc., can also promote the propagation and cartilaginification of fat stem cell.BMP-2 and insulin-like growth factor
Son -1 also plays a significant role in induced lipolysis stem cell cartilaginification, and wherein BMP-2 can promote Chondrocyte Differentiation, and up-regulation is soft
The expression of bone gene, promotes the reparation of cartilage damage；And IGF-1 is played by adjusting insulin-like growth factor-1 receptor
The effect of induced lipolysis stem cell cartilaginification.Mercenron etc. is investigated low-oxygen environment for fat stem cell cartilage differentiation
Influence, it is found that the low oxygen concentration that volume fraction is 5% can improve the content of II Collagen Type VI, and the morning of II Collagen Type VI can be promoted
Phase expresses., can be by being separated to human body autologous adipose tissue, being cut, cleaning fat is prepared with reference to the above
Stem cell, and then carry out the repairing and treating of Bones and joints.Since operation receiveing person itself can provide significant quantities of fat, packing material is of low cost
And be readily available, and transfer operation has invertibity, can repeatedly be injected when ineffective, adipocyte once survives can be forever
There is transplanting part long.But autologous fat tends to occur in the preparation that adipose tissue survival rate is low, and post-transplantation is easily inhaled
The situation of receipts, and there is the risk of liquefaction, infection, hemotoncus, necrosis, calcification, cystic fibrosis and false cyst so that operation effect
Fruit is subject to serious influence.Therefore autologous fat transplantation prepares SVF components key be that preparation transplant tissue during protect
Demonstrate,prove the survival rate of adipocyte：1) in extraction process, the tissue of extraction and the frequency of exposure of all kinds apparatus are reduced, is ensured
It is kept constantly in from suction lipectomy to injection process in the environment of closing and disinfection；2) the fatty suspension extracted must be through
Purifying, the particularly oil fraction except aging are crossed, the blood wherein mixed, swelling are removed and non-chemical method by physical means
Liquid, broken adipocyte and fibr tissue；Then the adipose tissue of preparation is uniformly refined using physical method, to
SVF is activated, improves the stem cell survival after transplanting.
The content of the invention
In order to overcome the shortcomings of during existing autologous fat transplantation that fat stem cell survival rate is low, transplant infection, this hair
It is bright that a kind of fat that can avoid drawbacks described above and the separation component containing vascular stroma simple and reliable for structure from adipose tissue is provided
Fat tissue preparation, the material of injection receptor site fat stem cell is prepared for physics.
The technical solution adopted by the present invention to solve the technical problems is：
A kind of adipose tissue preparation facilities that the component containing vascular stroma is separated from adipose tissue, described device include cleaning
Vibration separation cylindrical vessel, the cleaning vibration separation cylindrical vessel both ends are sealed with an inlet cap and the port of export respectively
Lid, the center location of the inlet cap are provided with entrance calparine cap, and the center location of the outlet end cap is provided with outlet liver
Plain cap, pacifies respectively in the cleaning vibration separation cylindrical vessel at the position of the inlet cap and the outlet end cap
Net is cut equipped with a tissue agglomerate, the sky of net and the cleaning vibration separation cylindrical vessel composition is cut in the tissue agglomerate
It is interior to be placed with auxiliary oscillating stainless shot.
Further, the cleaning vibration separation cylindrical vessel has three concavo-convex ripple clamp structures in barrel both sides respectively
It is described positioned at the barrel center of the cleaning vibration separation cylindrical vessel with a pair of of Photoelectric Detection window, the ripple clamp structure
Photoelectric Detection window is located at below the ripple clamp structure.
Further, the entrance calparine cap is three-port structure, and one leads to the adipose tissue for being connected to and being obtained from patient recipients
Container, one leads to and is connected to physiological saline container, and one leads to and is connected to the cleaning vibration separation cylindrical vessel, the entrance calparine cap
For check valve；The outlet calparine cap is three-port structure, and one leads to the collection vessel being connected to from transplant fat tissue is treated, one leads to
It is connected to waste liquid collection vessel, one leads to and is connected to the cleaning vibration separation cylindrical vessel, and the outlet calparine cap is check valve.
Further, the cleaning vibration separation cylindrical vessel is connected with the inlet cap, the outlet end cap outer rim
There is one of evagination step at place respectively, for the clamping position in instrument detection process.The Photoelectric Detection window is saturating for plane uniform thickness
Mirror, to separate cylindrical vessel axis direction parallel with the cleaning vibration for its plane.
Further, described device further includes photodetector system, and the photodetector system is sensed including photoemission
Device, photoelectric receiving transducer, microprocessor platform and detection support, the detection support are used to fix the cleaning vibration point
The Photoelectric Detection is separately fixed at from cylindrical vessel, the photoemission sensor and the photoelectric receiving transducer outside window
Side, the photoelectricity ray of the photoemission sensor emission vertically inject the Photoelectric Detection window of side, then vertical to project
In the Photoelectric Detection window of opposite side, finally received by the photoelectric receiving transducer.
Preferably, the tissue agglomerate cutting mesh screen hole aperture ratio positioned at inlet cap is located at described group of outlet end cap
Knit agglomerate cutting net screen-aperture it is big, the auxiliary oscillating stainless shot material selection is food-grade stainless steel.
The present invention technical concept be：Assembly of the invention provides easily and rapidly program, for example, from patient body
Suction of fat tissue, carries out the refinement, flushing, separation of tissue by way of physics, quantitative by introducing Photoelectric Detection means
The quality of autotransplantation injection fillers material that obtains every time of guarantee, and then improve the success rate of shaping filling, reduce bad
React odds.
Beneficial effects of the present invention are mainly manifested in：(1) device used is prepared using disposable apparatus, reduces life
The risk of thing material contamination, avoiding the defects of conventional appliances need washing, efficiency and long-time service causes deterioration
It is dangerous；(2) than the prior art, required biological filler can be obtained by simple mechanically actuated, improve and obtain
Obtain the efficiency of portable material；(3) automatic detection system is used, there is provided quantitative examination criteria, ensure that the biological material of transplanting
The reliability of material, improves the survival rate after biomaterial implantation.
Brief description of the drawings
Fig. 1 is to prepare the overall structure diagram that can fill adipose tissue device.
Fig. 2 is the overall structure diagram of cleaning vibration separation cylindrical vessel.
Fig. 3 is the Cylinder wall structure schematic diagram of cleaning vibration separation cylindrical vessel.
Fig. 4 is to prepare the flow diagram that can fill adipose tissue.
Embodiment
The invention will be further described below in conjunction with the accompanying drawings.
With reference to Fig. 1~Fig. 4, a kind of adipose tissue preparation facilities that the component containing vascular stroma is separated from adipose tissue, bag
Include cleaning vibration separation 1, inlet cap 2 of cylindrical vessel and one outlet end cap 3.
The inlet cap 2 and outlet end cap 3 seal the both ends for being fixed on cleaning vibration separation cylindrical vessel 1, institute respectively
The center location for stating inlet cap 2 is provided with entrance calparine cap 2-1, and the center location of the outlet end cap 3 is provided with outlet liver
Plain cap 3-1, the cutting of entrance tissue agglomerate is provided with the cleaning vibration separation cylindrical vessel 1 close to the inlet cap 2
1-5-1 is netted, outlet tissue agglomerate cutting is installed close to the outlet end cap 3 in the cleaning vibration separation cylindrical vessel 1
1-5-2 is netted, cuts in the entrance tissue agglomerate and is placed between net 1-5-1 and the outlet tissue agglomerate cutting net 1-5-2
Auxiliary oscillating stainless shot 1-6.
The barrel both sides of the cleaning vibration separation cylindrical vessel 1 have respectively three concavo-convex ripple clamp structure 1-2 and
A pair of of Photoelectric Detection window 1-4, the ripple clamp structure 1-2 divide in the barrel of the cleaning vibration separation cylindrical vessel 1
Point, the Photoelectric Detection window 1-4 are located at the ripple clamp structure 1-2 at 1cm below 2 direction of inlet cap.
The entrance calparine cap 2-1 is three-port structure check valve, and one leads to 2-1-1 to be connected to what is obtained from patient recipients
Adipose tissue container, to be connected to physiological saline container, one leads to and is connected to the cleaning vibration separation cylinder and holds a logical 2-1-2
In the cavity wall of device 1；The outlet calparine cap 3-1 is three-port structure check valve, and a logical 3-1-1 is being connected to from treating transplant fat
The collection vessel of tissue, a logical 3-1-2 are connected to waste liquid collection vessel, and one leads to and is connected to the cleaning vibration separation cylindrical vessel
In 1 cavity wall.
The cleaning vibration separation cylindrical vessel 1 is distinguished with the inlet cap 2,3 outer rim junction of the outlet end cap
There is one of evagination step 1-3, for the clamping position in instrument detection process.The Photoelectric Detection window 1-4 is saturating for plane uniform thickness
Mirror, to separate 1 container axis direction of cylinder parallel with the cleaning vibration for the plane of the Photoelectric Detection window 1-4.
Described device further includes photodetector system 4, and the photodetector system 4 includes photoemission sensor 4-1, light
Electric receiving sensor 4-2, microprocessor platform 4-3, detection support 4-4 and cleaning vibration separation cylindrical vessel fixture 4-5, institute
State cleaning vibration separation cylindrical vessel fixture 4-5 and the detection support 4-4, the cleaning vibration separation are fixed on by overarm arm
Cylindrical vessel fixture 4-5 is used to fix the cleaning vibration separation cylindrical vessel 1, the photoemission sensor 4-1 and described
Photoelectric receiving transducer 4-2, the photoemission sensor 4-1 and the photoelectric receiving transducer 4-2 are separately mounted to described
On the outside of Photoelectric Detection window 1-4, the photoelectricity ray of the photoemission sensor 4-1 transmittings vertically injects the photoelectricity inspection of side
Window 1-4-1 is surveyed, is then vertically projected in the Photoelectric Detection window 1-4-2 of opposite side, finally by the photoelectric receiving transducer
4-2 is received.
7 mesh of entrance tissue agglomerate cutting net 1-5-1 screen-apertures selected as, the outlet tissue agglomerate cutting net 1-
16 mesh of 5-2 screen-apertures selected as, the auxiliary oscillating stainless shot 1-6 material selections are 304 stainless steels, a diameter of 5mm, steel
The number of pearl is 5.
The operating process of adipose tissue material preparation can be filled by being described below.
1) preparation：The passage 3-1-1 for exporting calparine cap 3 using closing, opens the passage 2-1-2 of entrance calparine cap 2,
The passage 3-1-2 of outlet calparine cap 3 is opened, physiological saline 7-1 shakes from the passage 2-1-2 injections cleaning of entrance calparine cap 2
Separation cylindrical vessel 1 is swung, by the air in the cleaning vibration separation cylindrical vessel 1 by exporting the passage 3-1-2 of calparine cap 3
Discharge；
2) injection fat：The passage 2-1-2 of entrance calparine cap 2 is closed, opens the passage 2-1-1 of entrance calparine cap 2, is opened
The passage 3-1-2 of calparine cap 3 is exported, the syringe that storage has patient's liposuction aspirates 7-2 is passed through into the passage of entrance calparine cap 2
2-1-1 is injected into the cleaning vibration separation cylindrical vessel 1, and liposuction aspirates 7-2 is cut by the entrance tissue agglomerate
Refined after net 1-5-1 and by physics cutting, and the part physiology cleaned in vibration separation cylindrical vessel 1 is filled in by original
Brine 7-1 is discharged in waste collecting device by exporting the passage 3-1-2 of calparine cap 3, when patient's liposuction aspirates 7-2 is injected
After, the passage 2-1-1 of closing entrance calparine cap 2.
3) oscillation cleaning：The passage 2-1-2 of entrance calparine cap 2 is opened, opens the passage 3-1-2 of outlet calparine cap 3, will be dry
Net physiological saline 7-1 persistently injects the cleaning vibration separation cylindrical vessel 1, and rocks the cleaning vibration separation cylinder and hold
Device 1, in the case where the auxiliary oscillating stainless shot 1-6 rocks stirring, is uniformly mixed liposuction thing and physiological saline, due to fat
Fat tissue is not dissolved in water, and is stopped by the thinner outlet tissue agglomerate cutting net 1-5-2 of mesh, therefore the fat of processing to be cleaned
Fat tissue will stay in cleaning vibration separation 1 cavity of cylindrical vessel.Under the continuous flushing of physiological saline, extract in thing
Tissue fluid, watery blood, anaesthetic and cell fragment 7-3 constantly rinsed and by export calparine cap 3 passage 3-1-2 discharge
To waste collecting device.
4) detection is stood：After rinsing a period of time, the passage 3-1-2 of outlet calparine cap 3 is closed, closes entrance calparine cap 2
Passage 2-1-2 and stop saline injection, keep outlet end cap 3 side upward, by it is described cleaning vibration separation cylinder
Container 1 is placed on the cleaning vibration separation cylindrical vessel fixture 4-5 and stands, lipase 37-4 to be sucked and physiological saline 7-1
After layering, photodetector system 4 is run, microprocessor platform 4-3 is calculated by gathering the signal of photoelectric receiving transducer 4-2
The light transmittance of the physiological saline of stratification, and then judge whether the flushing of adipose tissue is completed.
5) after the completion of detecting, fatty packing material collection syringe is connected to the passage 3-1-1 of outlet calparine cap 3, and
The passage 3-1-1 of outlet calparine cap 3 is opened, the passage 2-1-2 of entrance calparine cap 2 is opened and continues to be injected into physiological saline 7-1,
At this time, due to outlet end cap 3 side upward and fat rest on it is described cleaning vibration separation cylindrical vessel 1 upper strata, noting
Under the impetus of jetting pressure, the fat that is purified prepare tissue by the outlet tissue agglomerate cutting net 1-5-2 and by into
Step refining cuts to be formed fills adipose tissue 7-5 for transplanting, continues up injection and enters fat stem cell collection note
Emitter, prepared by material completes.
Claims (6)
- A kind of 1. adipose tissue preparation facilities that the component containing vascular stroma is separated from adipose tissue, it is characterised in that：The dress Put and vibrate separation cylindrical vessel including cleaning, the cleaning vibration separation cylindrical vessel both ends are sealed with an inlet cap respectively And outlet end cap, the center location of the inlet cap are provided with entrance calparine cap, the center location installation of the outlet end cap There is outlet calparine cap, close to the position of the inlet cap and the outlet end cap in the cleaning vibration separation cylindrical vessel Place is separately installed with a tissue agglomerate cutting net, and net and the cleaning vibration separation cylindrical vessel are cut in the tissue agglomerate Auxiliary oscillating stainless shot is placed with the space of composition.
- 2. separating the adipose tissue preparation facilities of the component containing vascular stroma from adipose tissue as claimed in claim 1, it is special Sign is：The cleaning vibration separation cylindrical vessel has three concavo-convex ripple clamp structures and a pair of of light in barrel both sides respectively Electro-detection window, the ripple clamp structure is positioned at the barrel center of the cleaning vibration separation cylindrical vessel, the Photoelectric Detection Window is located at below the ripple clamp structure.
- 3. the adipose tissue preparation facilities of the component containing vascular stroma is separated from adipose tissue as claimed in claim 1 or 2, its It is characterized in that：The entrance calparine cap is three-port structure, and one leads to the adipose tissue container for being connected to and being obtained from patient recipients, and one is logical It is connected to physiological saline container, one leads to and is connected to the cleaning vibration separation cylindrical vessel, and the entrance calparine cap is check valve； The outlet calparine cap is three-port structure, and one leads to the collection vessel being connected to from transplant fat tissue is treated, one leads to and is connected to waste liquid Collection vessel, one lead to be connected to it is described cleaning vibration separation cylindrical vessel, it is described outlet calparine cap be check valve.
- 4. the adipose tissue preparation facilities of the component containing vascular stroma is separated from adipose tissue as claimed in claim 1 or 2, its It is characterized in that：The cleaning vibration separation cylindrical vessel has respectively with the inlet cap, the outlet end cap outer rim junction One of evagination step, for the clamping position in instrument detection process, the Photoelectric Detection window is the thick lens such as plane, its plane It is parallel that cylindrical vessel axis direction is separated with the cleaning vibration.
- 5. the adipose tissue preparation facilities of the component containing vascular stroma is separated from adipose tissue as claimed in claim 1 or 2, its It is characterized in that：Described device further includes photodetector system, and the photodetector system includes photoemission sensor, and photoelectricity connects Sensor, microprocessor platform and detection support are received, the detection support is held for fixing the cleaning vibration separation cylinder Device, the photoemission sensor and the photoelectric receiving transducer are separately fixed at Photoelectric Detection side outside window, the light The photoelectricity ray of electric emission sensor transmitting vertically injects the Photoelectric Detection window of side, then vertically projects in opposite side The Photoelectric Detection window, is finally received by the photoelectric receiving transducer.
- 6. the adipose tissue preparation facilities of the component containing vascular stroma is separated from adipose tissue as claimed in claim 1 or 2, its It is characterized in that：Tissue agglomerate cutting mesh screen hole aperture ratio positioned at inlet cap is located at the tissue agglomerate of outlet end cap Cutting net screen-aperture is big, and the auxiliary oscillating stainless shot material selection is food-grade stainless steel.
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| CN201711070368.5A CN107904138A (en) | 2017-11-03 | 2017-11-03 | The adipose tissue preparation facilities of the component containing vascular stroma is separated from adipose tissue |
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| CN201711070368.5A CN107904138A (en) | 2017-11-03 | 2017-11-03 | The adipose tissue preparation facilities of the component containing vascular stroma is separated from adipose tissue |
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