CN107898876A - Eutherapeutic plate green grass or young crops particle and preparation method thereof - Google Patents
Eutherapeutic plate green grass or young crops particle and preparation method thereof Download PDFInfo
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- CN107898876A CN107898876A CN201711371127.4A CN201711371127A CN107898876A CN 107898876 A CN107898876 A CN 107898876A CN 201711371127 A CN201711371127 A CN 201711371127A CN 107898876 A CN107898876 A CN 107898876A
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- green grass
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/19—Acanthaceae (Acanthus family)
- A61K36/195—Strobilanthes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
- A61K36/315—Isatis, e.g. Dyer's woad
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
- A61K36/704—Polygonum, e.g. knotweed
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/85—Verbenaceae (Verbena family)
- A61K36/855—Clerodendrum, e.g. glorybower
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
- A61K9/1605—Excipients; Inactive ingredients
- A61K9/1629—Organic macromolecular compounds
- A61K9/1652—Polysaccharides, e.g. alginate, cellulose derivatives; Cyclodextrin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
- A61K9/1682—Processes
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- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
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Abstract
The present invention provides a kind of eutherapeutic plate green grass or young crops particle and preparation method thereof, the plate green grass or young crops particle greatly improves the bacteriostasis of the pathogenic strains such as Streptococcus suis, Escherichia coli.The present invention adds glycine betaine, the chitosan of small-molecular-weight, sodium tripolyphosphate during extraction is decocted, the plate green grass or young crops particle obtained after these processing is significantly improved the bacteriostatic activity of livestock and poultry pathogenic bacteria compared with the common process recorded on Chinese Pharmacopoeia.In addition, it has been found that the addition sequence of adjustment glycine betaine, chitosan and sodium tripolyphosphate, i.e., first add sodium tripolyphosphate, add chitosan, be eventually adding glycine betaine, can accelerate the melting of plate green grass or young crops particle, being stirred 1 minute after being added to the water all to dissolve；It also can further improve fungistatic effect of the plate green grass or young crops particle to B.bronchisepticai and Escherichia coli at the same time.
Description
Technical field
The present invention relates to a kind of Chinese medicine preparation and preparation method thereof, more particularly to a kind of eutherapeutic plate green grass or young crops particle and its system
Preparation Method.
Background technology
Plate green grass or young crops particle is using Radix Isatidis and folium isatidis as a kind of pure Chinese medicinal preparation of main component, is had clearing heat and detoxicating, cool
The function of blood, cures mainly anemopyretic cold, abscess of throat, pyreticosis hair spot isothermal febrile illness.It is popular sexy that plate green grass or young crops particle is suitable for pig
Emit, smoothing filter operator, blue otopathy, chicken non typical newcastle disease, gumboro disease, infectious laryngotracheitis, influenza etc., especially
Suitable for animal temperature febrile illness caused by torridity summer, hot and humid etc..
The production technology of plate green grass or young crops particle is usually to be decocted after mixing Radix Isatidis and folium isatidis at present, and filtrate is concentrated to give
To thick paste mixed with auxiliary material after pelletize.But it is larger using the loss of effective components in decocting method medicinal material, can be in certain journey
Drug effect is influenced on degree.Other methods such as THROUGH METHOD, circumfluence method, combined-enzyme method etc., there are the shortcomings of of high cost, complex process.
The content of the invention
It is an object of the invention to provide a kind of eutherapeutic plate green grass or young crops particle and preparation method thereof, the plate green grass or young crops particle is to pig
The bacteriostasis of the pathogenic strains such as streptococcus, Escherichia coli greatly improves.
The technical solution adopted by the present invention is：
A kind of preparation method of eutherapeutic plate green grass or young crops particle, comprises the following steps：By 900 parts by weight folium isatidis and 600 weights
The mixing of part Radix Isatidis is measured, the water of 10 times of its gross weight is then added, sodium tripolyphosphate, chitosan, sweet tea is sequentially added after boiling
Dish alkali, then decocts 2 times, each 1h for 100 DEG C, collecting decoction, filtering, and filtrate is concentrated into paste, adds sucrose, dextrin mixes
It is even, particle is made, it is dry, 1500g is made, you can.
The molecular weight of the chitosan is 5-18 ten thousand, and dosage is the 2-3% of folium isatidis weight；
The dosage of the sodium tripolyphosphate is the 0.1-0.5% of folium isatidis weight；
The dosage of the glycine betaine is the 0.1-0.5% of folium isatidis weight；
Filtrate described in step (2) is concentrated into paste, adds sucrose, dextrin mixing, particle is made, dry, is made
1500g, it is the ordinary skill in the art and operation.
The present invention adds glycine betaine, the chitosan of small-molecular-weight, sodium tripolyphosphate during extraction is decocted, and passes through
The plate green grass or young crops particle obtained after these processing is compared with the common process recorded on Chinese veterinary pharmacopoeia, the antibacterial work to livestock and poultry pathogenic bacteria
Property is significantly improved.In addition, it has been found that the addition sequence of adjustment glycine betaine, chitosan and sodium tripolyphosphate, i.e., first add
Sodium tripolyphosphate, adds chitosan, is eventually adding glycine betaine, can accelerate the melting of plate green grass or young crops particle, and 1 is stirred after being added to the water
Minute can all dissolve；Plate green grass or young crops particle also be can further improve at the same time to B.bronchisepticai and Escherichia coli
Fungistatic effect.
The advantage of the invention is that：Antibacterial work to pathogenic bacteria especially B.bronchisepticai and Escherichia coli
Property higher, better efficacy, and dissolution velocity is fast in water, using more convenient.
Embodiment
Embodiment 1：A kind of preparation method of eutherapeutic plate green grass or young crops particle, comprises the following steps：By 900 parts by weight folium isatidis
Mixed with 600 parts by weight Radix Isatidis, then add the water of 10 times of its gross weight, sodium tripolyphosphate, shell are sequentially added after boiling
Glycan, glycine betaine, then decoct 2 times, each 1h for 100 DEG C, collecting decoction, filtering, and filtrate is concentrated into paste, add sucrose,
Dextrin mixes, and particle is made, dry, and 1500g is made, you can.
The molecular weight of the chitosan is 5-18 ten thousand, and dosage is the 2.5% of folium isatidis weight；
The dosage of the sodium tripolyphosphate is the 0.2% of folium isatidis weight；
The dosage of the glycine betaine is the 0.3% of folium isatidis weight；
Embodiment 2：It is with the difference of embodiment 1：
The molecular weight of the chitosan is 5-18 ten thousand, and dosage is the 2% of folium isatidis weight；
The dosage of the sodium tripolyphosphate is the 0.5% of folium isatidis weight；
The dosage of the glycine betaine is the 0.5% of folium isatidis weight；
Remaining is the same as embodiment 1.
Embodiment 3：It is with the difference of embodiment 1：
The molecular weight of the chitosan is 5-18 ten thousand, and dosage is the 3% of folium isatidis weight；
The dosage of the sodium tripolyphosphate is the 0.1% of folium isatidis weight；
The dosage of the glycine betaine is the 0.1% of folium isatidis weight；
Remaining is the same as embodiment 1.
Embodiment 4：It is with the difference of embodiment 1：The molecular weight of the chitosan is 19-37 ten thousand, remaining is the same as real
Apply example 1.
Embodiment 5：It is with the difference of embodiment 1：Sodium tripolyphosphate is not added, remaining is the same as embodiment 1.
Embodiment 6：It is with the difference of embodiment 1：The dosage of the glycine betaine is the 0.6% of folium isatidis weight；
Remaining is the same as embodiment 1.
Embodiment 7：It is with the difference of embodiment 1：
A kind of preparation method of eutherapeutic plate green grass or young crops particle, comprises the following steps：By 900 parts by weight folium isatidis and 600 weights
The mixing of part Radix Isatidis is measured, the water of 10 times of its gross weight is then added, chitosan, sodium tripolyphosphate, sweet tea is sequentially added after boiling
Dish alkali, then decocts 2 times, each 1h for 100 DEG C, collecting decoction, filtering, and filtrate is concentrated into paste, adds sucrose, dextrin mixes
It is even, particle is made, it is dry, 1500g is made, you can.
Remaining is the same as embodiment 1.
Embodiment 8：It is with the difference of embodiment 1：
A kind of preparation method of eutherapeutic plate green grass or young crops particle, comprises the following steps：By 900 parts by weight folium isatidis and 600 weights
The mixing of part Radix Isatidis is measured, the water of 10 times of its gross weight is then added, sodium tripolyphosphate, glycine betaine, shell is sequentially added after boiling
Glycan, then decocts 2 times, each 1h for 100 DEG C, collecting decoction, filtering, and filtrate is concentrated into paste, adds sucrose, dextrin mixes
It is even, particle is made, it is dry, 1500g is made, you can.
Remaining is the same as embodiment 1.
Comparative example 1：It is with the difference of embodiment 1：The preparation method of plate green grass or young crops particle is same《People's Republic of China (PRC) beast
Pharmacopeia》2010 editions two ministerial standards, i.e., by Radix Isatidis 600g, folium isatidis 900g, add the water of 10 times of water gross weight to decoct 2 times, and every time 1
Hour, collecting decoction, filtration, filtrate is concentrated into paste, adds sucrose, appropriate dextrin, mixes, particle is made, dry, is made
1500g, to obtain the final product.That is, being added without sodium tripolyphosphate, glycine betaine, chitosan in decoction process, remaining is the same as embodiment 1.
Test example 1：Fungistatic effect compares
Bacteriostatic experiment carries out as follows：
(1) by Escherichia coli, staphylococcus aureus, micrococcus scarlatinae, pig pleura Actinobacillus, pig branch gas
Pipe sepsis bordetella bacilli is inoculated in MH broth bouillon cultures, and strain after above-mentioned activation is inoculated in slant medium respectively,
Put cultivated in 37 DEG C of incubators 18-24 it is small when, strain in slant medium is diluted with nutrient broth, makes its bacterial concentration be
106CFU/ml bacterium solutions, it is spare.
(2) plate each 5g of green grass or young crops particle made from Example 1-8 and comparative example 1, is placed in 150m1 triangular flasks, uses distilled water
The sample solution of the particle of green grass or young crops containing plate 0.5g/ml is made into, autoclaving is placed on 4 DEG C of refrigerators and saves backup.
(3) assay method of MIC：Sterile test tube 10 is taken, adds 1ml sterile distilled waters, takes sample solution 1.0ml to move into
No. 1 test tube, after mixing, carries out gradient dilution, No. 10 Guan Weiwu medicines controls.Culture observation is carried out with 50%MH meat soups, without thin
The contained drug Cmin of bacterium growth is minimal inhibitory concentration (MIC).
The assay method of MBC：Sterile test tube 10 is taken, adds 1ml sterile distilled waters, takes sample extracting solution 1.0ml to move into 1
Number test tube, after mixing, carries out gradient dilution, No. 10 Guan Weiwu medicines controls.Every test tube adds thawing to 55 DEG C of solid fine jades
Fat culture medium (9ml) fully mix, pour plate, takes bacteria suspension to be applied to planar surface, put 37 DEG C culture 18-24 it is small when, culture
Contained drug concentration is minimum bactericidal concentration (MBC) in tablet of the bacterium colony less than 5 afterwards.
1 plate green grass or young crops particle of the table MIC that is averaged compares (g/ml)
| Packet | Escherichia coli | Staphylococcus aureus | Micrococcus scarlatinae | B.bronchisepticai |
| Comparative example 1 | 0.381 | 0.403 | 0.379 | 0.425 |
| Embodiment 1 | 0.154 | 0.178 | 0.196 | 0.151 |
| Embodiment 2 | 0.159 | 0.181 | 0.198 | 0.155 |
| Embodiment 3 | 0.156 | 0.180 | 0.201 | 0.157 |
| Embodiment 4 | 0.382 | 0.399 | 0.382 | 0.421 |
| Embodiment 5 | 0.390 | 0.405 | 0.375 | 0.429 |
| Embodiment 6 | 0.384 | 0.402 | 0.376 | 0.432 |
| Embodiment 7 | 0.242 | 0.178 | 0.196 | 0.258 |
| Embodiment 8 | 0.245 | 0.178 | 0.196 | 0.256 |
2 plate green grass or young crops particle of the table MBC that is averaged compares (g/ml)
Test example 2：Melting compares
Distinguish plate each 10g of green grass or young crops particle made from Example 1-8 and comparative example 1, heat water 200ml, noted in whipping process
The time that meaning observation each group plate green grass or young crops particle all dissolves, it the results are shown in Table 3.
3 plate green grass or young crops particle solution time of table compares
| Packet | Solution time (min) |
| Comparative example 1 | 5min |
| Embodiment 1 | 1min |
| Embodiment 2 | 1min |
| Embodiment 3 | 1min |
| Embodiment 4 | 5min |
| Embodiment 5 | 5min |
| Embodiment 6 | 4min |
| Embodiment 7 | 5min |
| Embodiment 8 | 4.5min |
Claims (4)
- A kind of 1. preparation method of eutherapeutic plate green grass or young crops particle, it is characterised in that：Comprise the following steps：By 900 parts by weight smalts Leaf and the mixing of 600 parts by weight Radix Isatidis, then add the water of 10 times of its gross weight, sequentially added after boiling sodium tripolyphosphate, Chitosan, glycine betaine, then decoct 2 times, each 1h for 100 DEG C, collecting decoction, filtering, and filtrate is concentrated into paste, adds sugarcane Sugar, dextrin mix, and particle is made, dry, and 1500g is made, you can.
- A kind of 2. preparation method of eutherapeutic plate green grass or young crops particle as claimed in claim 1, it is characterised in that：The chitosan Molecular weight is 5-18 ten thousand, and dosage is the 2-3% of folium isatidis weight；The dosage of the sodium tripolyphosphate is the 0.1- of folium isatidis weight 0.5%；The dosage of the glycine betaine is the 0.1-0.5% of folium isatidis weight.
- A kind of 3. preparation method of eutherapeutic plate green grass or young crops particle as claimed in claim 1, it is characterised in that：The chitosan Molecular weight is 5-18 ten thousand, and dosage is the 2.5% of folium isatidis weight；The dosage of the sodium tripolyphosphate is folium isatidis weight 0.2%；The dosage of the glycine betaine is the 0.3% of folium isatidis weight.
- A kind of 4. preparation method of eutherapeutic plate green grass or young crops particle as claimed in claim 1, it is characterised in that：Add in decoction process Sodium tripolyphosphate is entered.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711371127.4A CN107898876A (en) | 2017-12-19 | 2017-12-19 | Eutherapeutic plate green grass or young crops particle and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711371127.4A CN107898876A (en) | 2017-12-19 | 2017-12-19 | Eutherapeutic plate green grass or young crops particle and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107898876A true CN107898876A (en) | 2018-04-13 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711371127.4A Pending CN107898876A (en) | 2017-12-19 | 2017-12-19 | Eutherapeutic plate green grass or young crops particle and preparation method thereof |
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| CN (1) | CN107898876A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115177679A (en) * | 2022-07-01 | 2022-10-14 | 山东琪康生物技术有限公司 | Preparation process of veterinary isatis root granules for improving content of effective ingredients and palatability |
-
2017
- 2017-12-19 CN CN201711371127.4A patent/CN107898876A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115177679A (en) * | 2022-07-01 | 2022-10-14 | 山东琪康生物技术有限公司 | Preparation process of veterinary isatis root granules for improving content of effective ingredients and palatability |
| CN115177679B (en) * | 2022-07-01 | 2023-05-09 | 山东琪康生物技术有限公司 | Preparation technology of veterinary isatis root granules for improving content and palatability of active ingredients |
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Application publication date: 20180413 |