CN107898670A - A kind of spot-dispelling liquid and preparation method thereof - Google Patents
A kind of spot-dispelling liquid and preparation method thereof Download PDFInfo
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- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
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- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
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Abstract
The present invention relates to spot-dispelling liquid processing technique field, more particularly to a kind of spot-dispelling liquid and preparation method thereof, the spot-dispelling liquid of the application are made of potato composite essential oil, ginkgo biloba p.e, mulberry-leaf extract, apple seed extract, propolis extract, malt embryo extract, hyaluronic acid and olive oil；In spot-dispelling liquid, potato composite essential oil includes potato derived essential oil and auxiliary material derived essential oil, by potato essential oil and auxiliary material essential oil compounding use, can improve the DPPH free radical scavenging activities of potato derived essential oil, improve the inoxidizability of essential oil；The inoxidizability of spot-dispelling liquid can be effectively improved in spot-dispelling liquid containing ginkgo biloba p.e, mulberry-leaf extract, apple seed extract, propolis extract, malt embryo extract, hyaluronic acid and olive oil, improve the stability of potato essential oil, the light sensitivity of potato essential oil is reduced, improves freckle effect.
Description
【Technical field】
The present invention relates to spot-dispelling liquid processing technique field, more particularly to a kind of spot-dispelling liquid and preparation method thereof.
【Background technology】
The main nti-freckle component of spot-dispelling liquid on the market be mainly citric acid or lemon extract etc. rich in it is ascorbic into
Point, although vitamin C, with very strong inoxidizability, can have makes the colour of skin bleach, and has good freckle effect, dimension is given birth to
Plain C has very strong photosensitization, if improper use can deepen melanin deposition on the contrary under sunlight irradiation, causes skin
Long spot；And in potato essential oil, also containing abundant vitamin C in addition to containing derived essential oil, although having good whitening
Effect, but improper use will cause melanin deposition under sunlight irradiation, can run counter to desire, therefore, if by horse
Bell potato essential oil is applied with whitening spot-removing, it is necessary to rationally be compounded to potato essential oil, the antidecomposition energy of raising potato essential oil
Power, can efficiently use the strong anti-oxidation of potato essential oil, while will not also cause minus effect.
【The content of the invention】
In view of the above, it is necessary to produce a kind of spot-dispelling liquid, potato essential oil can rationally be compounded, improve horse
The antidecomposition ability of bell potato essential oil, can efficiently use the strong anti-oxidation of potato essential oil, while will not also cause minus effect.
To reach above-mentioned purpose, the technical solution adopted in the present invention is：
A kind of spot-dispelling liquid, the spot-dispelling liquid comprise the following components in parts by weight：20 parts -30 parts of potato composite precision
Oil, 15 parts -25 parts of ginkgo biloba p.e, 11 parts -21 parts of mulberry-leaf extract, 13 parts -28 parts of apple seed extract, 17
Parts -29 parts of propolis extract, 13 parts -28 parts of malt embryo extract, 25 parts -38 parts of hyaluronic acid and 14 parts -25 parts
Olive oil.
Further, potato essential oil and weight of the potato composite essential oil by percentage by weight for 70%-90%
The auxiliary material essential oil that percentage is 10%-30% forms.
Further, the potato essential oil is made by potato；The auxiliary material essential oil by following parts by weight component system
：17 parts -29 parts of Chinese toon wood skin, 13 parts -31 parts of Radix agastaches, 24 parts -32 parts of Semen sesami nigrum and 34 parts -46 parts of the leaf of bamboo.
Further, the potato composite essential oil preparation method include potato extraction of essential oil, auxiliary material extraction of essential oil and
Essential oil mixes three steps and is made, and comprises the following steps that：
(1) potato extraction of essential oil：
Step S1：Potato is puffing：Potato is cut into the potato block that thickness is 1-3mm, then potato block is put into
Temperature is to soak 1-2h in 0-2 DEG C of frozen water, potato block is taken out drain be totally submerged keep 1-2s in rapeseed oil after take
Go out, be put into steamer cook, mashed into paste, it is 9MPa-10MPa that mashed potato then is put into pressure condition, temperature 100
Keep 10min-15min in DEG C -105 DEG C of hot wind, pressure release, then it is 0MPa-2MPa that mashed potato is put into pressure condition, temperature
It is puffing to keep 5min-10min to complete potato in 0 DEG C -5 DEG C of cold wind；
Step S2：Cellulase treatment：By potatos of the step S1 after puffing and biology enzyme I solution according to solid-liquid mass ratio
For 1:1-2 mixing immersions, 20min-30min is stood in the insulating box that temperature is 27 DEG C, is then concentrated, drying goes to remove water
Get potato flour；Wherein, biology enzyme I solution is made of the raw material of following parts by weight：30 parts -35 parts of amylase, 20
Parts -25 parts carbohydrase, 5 parts -10 parts of aspergillus oryzae, 10 parts -20 parts of barbaloin and 250 parts of -300 parts of mass percents are
1% sodium hydroxide solution；The enzyme activity of the amylase is 800u/g-1000u/g；The enzyme activity of the carbohydrase is
1000u/g-1200u/g；The living bacteria count of the aspergillus oryzae is 3.1 × 107~6.7 × 107CFU/g；
Step S3：Microwave treatment：After the potato flour of step S2 is cooled to room temperature, it is laid in plate, tiling is thick
Spend for 1mm-3mm, be placed on microwave oven turntable and carry out microwave treatment, the frequency of the microwave treatment is 2300MHZ, and power is
1000W, total duration 180s；
Step S4：Supercritical CO2Extraction：By in the potato flour input extraction kettle after step S3 microwave treatments, pressing
When power is 15-20MPa, with liquid CO2Static extracting 30-40min, then progressively discharges pressure, carries out supercritical CO2Dynamic extracts
Take, dynamic extraction technological parameter is：When temperature is heated to 40-50 DEG C, pressurize to system, pressure reaches 40-50MPa
Afterwards, CO is adjusted2Flow is 15-25L/min, keeps constant temperature and pressure to discharge after carrying out cycling extraction 2-4h, in 800-1000r/min
Under conditions of be centrifuged to obtain potato essential oil.
(2) auxiliary material extraction of essential oil：
Step A1：Auxiliary material is puffing：Each raw material is weighed by above-mentioned parts by weight to be crushed respectively, by -50 mesh sieve net of 30 mesh
Screening, is then 1 according to solid-liquid mass ratio by ethanol solution that Chinese toon wood skin powder and percentage by volume are 75%:2 mixing；By the leaves of pulse plants
The butanol solution that spiceleaf powder is 10% with percentage by volume is 1 according to solid-liquid mass ratio:1 mixing；By Semen sesami nigrum powder and body
The ethanol solution that product percentage is 75% is 1 according to solid-liquid mass ratio:1 mixing；By leaf of bamboo powder and water according to solid-liquid mass ratio
For 1:1 mixing；Then each powder soak is placed in the refrigerator-freezer that temperature is -2 DEG C~0 DEG C and refrigerates 24h-26h；Filtering, takes each
Powder mixes, and it is 8MPa-9MPa to be put into after being infiltrated in rapeseed oil mixed-powder being put into pressure condition, and temperature is 110 DEG C -120
DEG C hot wind in keep 20min-25min, pressure release, then it is 0MPa-2MPa that mashed potato is put into pressure condition, temperature for 0 DEG C-
15min-20min is kept to complete auxiliary material in 5 DEG C of cold wind puffing；
Step A2：Cellulase treatment：By auxiliary materials of the step S1 after puffing and biology enzyme II solution according to solid-liquid mass ratio
For 1:1-2 mixing immersions, 20min-30min is stood in the insulating box that temperature is 27 DEG C, is then concentrated, drying goes to remove water
Get auxiliary material dry powder；Wherein, biology enzyme II solution is made of the raw material of following parts by weight：15 parts -25 parts of pectase, 30
Cellulase, 20 parts -30 parts of amylase, 10 parts -20 parts of aspergillus flavus and 300 parts of -350 parts of mass percents of -50 parts of part
For 2% sodium chloride solution；The enzyme activity of the pectase is 1200u/g-1500u/g；The enzyme activity of the cellulase is
1500u/g-2000u/g；The enzyme activity of the amylase is 900u/g-1200u/g；The living bacteria count of the aspergillus flavus is
3.9×108~6.7 × 108CFU/g；
Step A3：Supercritical CO2Extraction：The auxiliary material dry powder of step S2 is put into extraction kettle, is 15-20MPa in pressure
When, with liquid CO2Static extracting 20-30min, then progressively discharges pressure, carries out supercritical CO2Dynamic extraction, dynamic extraction work
Skill parameter is：When temperature is heated to 50-60 DEG C, pressurize to system, after pressure reaches 30-40MPa, adjust CO2Flow
For 15-25L/min, keep constant temperature and pressure to discharge after carrying out cycling extraction 3-5h, carried out under conditions of 800-1000r/min from
The isolated auxiliary material essential oil of the heart.
(3) essential oil mixes：
Potato essential oil and auxiliary material essential oil are weighed according to percentage by weight, is 75% by potato essential oil and percentage by volume
Ethanol solution according to 2:1 is mixed, and 5min-7min is stirred under the speed conditions of 500-800r/min, is then added
Auxiliary material essential oil, continues to stir 6min-8min, rotary evaporation concentration goes moisture removal that potato composite precision described herein is made
Oil.
Present invention also provides a kind of preparation method for preparing spot-dispelling liquid, this method comprises the following steps：
(1) each material composition is weighed by the parts by weight；
(2) potato composite essential oil is mixed with olive oil, and is put into stirring in homogenizer and emulsion is made, it is described
Mixing time is 25min-35min；Speed of agitator is 700r/min-900r/min；Then emulsion is put into Ultrasound Instrument again
It is ultrasonically treated, sonification power 800w-1000w, sonication treatment time 10min-20min, is ultrasonically treated temperature
For 50 DEG C~60 DEG C, it is cooled to after room temperature and emulsion is put into temperature to be freezed in -7 DEG C~-5 DEG C refrigerator-freezers, cooling time
Emulsion after being freezed for 8h-12h；
(3) step (2) emulsion is put into the water-bath that temperature is 80 DEG C~90 DEG C and heats 30min-40min, Ran Houzai
Add ginkgo biloba p.e, mulberry-leaf extract, apple seed extract and malt embryo extract and be put into homogenizer and carry out
Stirring, mixing time 10min-15min, speed of agitator 1000r/min-1200r/min；Placed into after stirring transparent
Matter acid and propolis extract are uniformly mixed, and stir 5min- in the mixer that speed of agitator is 500r/min-700r/min
10min；Spot-dispelling liquid crude mixture is obtained, spot-dispelling liquid crude mixture is put into ceramic bottle and carries out sealing ageing 16d-22d, is obtained
The spot-dispelling liquid.
The present invention has the advantages that：
1st, the spot-dispelling liquid of the application is extracted by potato composite essential oil, ginkgo biloba p.e, mulberry-leaf extract, Apple Seeds
Thing, propolis extract, malt embryo extract, hyaluronic acid and olive oil composition, in spot-dispelling liquid, potato composite essential oil include from
The derived essential oil extracted in potato and the auxiliary material derived essential oil extracted from auxiliary material, are answered by potato essential oil and auxiliary material essential oil
With use, the DPPH free radical scavenging activities of potato derived essential oil can be improved, improve the inoxidizability of essential oil.In potato essential oil
The essential oil of extraction contains abundant lactone component, can effectively improve the essential oil extracted in essential oil fragrance Chinese toon wood skin and relatively be enriched with containing oxidation
Compound, can improve the oxidation resistance of essential oil；The essential oil extracted in Radix agastaches can improve killing for essential oil compared with beneficiation of hydrocarbons compound
Bacterium ability, so as to improve oxidation resistance；The essential oil extracted in Semen sesami nigrum is relatively enriched with oxygenatedchemicals component, so as to improve antioxygen
Change ability；The essential oil extracted in the leaf of bamboo is relatively enriched with oxygenatedchemicals component, so as to improve oxidation resistance.When auxiliary material derived essential oil
In be used in conjunction with each other containing Chinese toon wood skin, Semen sesami nigrum and several components of the leaf of bamboo after, can significantly improve the oxygenatedchemicals of essential oil into
Divide the oxidation resistance for improving essential oil, so that the ability with the very strong external tyrosinase activity of suppression.
2nd, although the application potato composite essential oil has very strong oxidation resistance, but wherein containing abundant dimension life
Plain C components, the component easily decompose under light illumination, it will cause melanin deposition, therefore, how to improve the stabilization of spot-dispelling liquid
Property, photosensitization is reduced, is the research potato composite essential oil spot-dispelling liquid technical issues that need to address；The ginkgo leaf of the application carries
The oxidation resistance of spot-dispelling liquid can be effectively improved containing Gingko yellow ketoside component in abundant extract, the component by taking in thing；Mulberry
Flavones in mulberry leaves, the mulberry leaf polyphenol component in leaf extract can effectively remove people's interior free yl, can effectively improve spot-dispelling liquid
Inoxidizability, the mulberry leaf polysaccharide in mulberry leaf have very strong bridging property, can be crosslinked by each component, can effectively improve spot-dispelling liquid
Stability；Containing abundant essential oil and vitamin C component in Apple Seeds, antioxygen rate can be improved；Vitamin E therein at the same time
Component can effectively improve inoxidizability, and ascorbic stability can be improved after being crosslinked with vitamin C, with heat resistanceheat resistant, antiacid, anti-
Photosensitizing effect；Containing colloid is enriched in propolis extract, the crosslinking ability of each derived essential oil such as potato essential oil can be effectively improved,
There is very strong anti-photosensitizing effect；Contain Abundant protein mass-energy bright color in malt embryo extract, due in potato essential oil
Vitamin C component is more, can't be only crosslinked completely with vitamin C with the vitamin E in Apple Seeds, and in malt embryo extract
Vitamin E and the vitamin Es of Apple Seeds can just be fully crosslinked with vitamin C, the inoxidizability of spot-dispelling liquid can be improved, improved
The stability of potato essential oil, reduces the light sensitivity of potato essential oil, improves freckle effect.
3rd, the application potato first carries out puffing, microwave treatment and cellulase treatment during essential oil is extracted, due to
Its essential oil of the potato of the application and Vitamin C content ratio starch and protein content component are low, and starch, protein material
With viscosity, the small-molecule substance such as essential oil and vitamin C cannot be separated out effectively, if, it is impossible to its potato is pre-processed
The output capacity of essential oil will be largely effected on, is soaked in the present frozen water of potato, can remove most starch material, Ma Ling
Potato chips will cause most starch chain and protein hydrogen bonds to be broken after high pressure, low pressure are puffing, with biology after crushing
Enzyme and microwave are handled, and can effectively remove content of starch, destroy the molecular structure of starch and protein, and this temperature, pressure
Power will not destroy derived essential oil again, will can effectively facilitate the precipitation efficiency of essential oil, improve the essential oil yield of potato；Meanwhile this
Apply for the supercritical CO used2Extraction prepares potato essential oil, and this method is easy to operate, ripe, and preparation rate is high, purity
It is high miscellaneous few；The characteristics of the application is directed to starch in potato, protein content is more is configured with biology enzyme I solution, forms sediment in enzyme solutions
Powder enzyme can effectively starch-splitting, carbohydrase starch can be decomposed into the glucose of small molecule, aspergillus oryzae containing abundant viable bacteria into
Point, can effective decomposing protein, barbaloin can effectively improve aspergillus oryzae activity, sodium hydroxide solution can be biology enzyme I solution carry
For enzymolysis environment preferably；The characteristics of it is mostly plant component that the application, which is directed in auxiliary material, and content of cellulose is high is configured with biology
Enzyme II solution, pectase can effectively decompose the pectin composition in plant in enzyme solutions, and cellulase can be decomposed effectively in plant
Cellulose components, amylase can effectively decompose starch enzyme component in plant, in aspergillus flavus containing abundant viable bacteria component, can be effective
Promote the decomposition of cellulose, sodium chloride solution can provide enzymolysis environment preferably for biology enzyme II solution.
【Embodiment】
All features disclosed in this specification, or disclosed all methods or during the step of, except mutually exclusive
Feature and/or step beyond, can combine in any way.
Any feature disclosed in this specification (including any accessory claim, summary), unless specifically stated, each
Feature is an example in a series of equivalent or similar characteristics.
Embodiment 1：
The present embodiment spot-dispelling liquid comprises the following components in parts by weight：20 parts of potato composite essential oil, 15 parts of ginkgo
Leaf extract, 11 parts of mulberry-leaf extract, 13 parts of apple seed extract, 17 parts of propolis extract, 13 parts of Fructus Hordei Germinatus carry
Take thing, 25 parts of hyaluronic acid and 14 parts of olive oil.
The extracting method of ginkgo biloba p.e is in the present embodiment：Fresh ginkgo leaf is shredded, then with the fourth of 200mg/g
Alcoholic solution is 1 according to solid-liquid mass ratio:2 are mixed, and are soaked 4 times, each 1h in 50 DEG C of water-bath medium temperatures, filtering, merging filtrate,
Vacuum distillation concentration, until concentrate obtains the ginkgo biloba p.e for the 1/3 of stoste, Gingko yellow ketoside contains in extract
Measure as 108.97mg/g.
The extracting method of mulberry-leaf extract is in the present embodiment：New fresh mulberry leaf is shredded, is 75% with percentage by volume
Ethanol is 1 according to mass ratio:4 are mixed, and refluxing extraction is then carried out in refluxing extraction device, and Extracting temperature is 90 DEG C, is carried
It is 2h to take the time, filters to take filtrate and carries out rotary evaporation concentration, until concentrate obtains the mulberry leaf extraction for the 1/2 of stoste
Thing, in extract flavones in mulberry leaves content be 96.57mg/g, mulberry leaf polyphenol 87.64mg/g, mulberry leaf polysaccharide 102.85mg/g.
The extracting method of apple seed extract is in the present embodiment：The Apple Seeds that moisture content is 5% are crushed to 80 mesh, are obtained
To Apple Seeds powder；Then it is 1 in mass ratio by Apple Seeds powder petroleum ether：2 ratio extracts 2h under 80 DEG C of temperature conditionss,
Period stirs 1 time every 10min, obtains leaching liquor, filters to take filtrate and obtain crude extract；It is by solid-liquid mass ratio into crude extract
1:100 add atlapulgite, after persistently stirring 5min, stand 40min, filter to take filtrate and obtain decoloration crude extract；Decoloration slightly carries
Liquid is placed on that temperature is 80 DEG C, vacuum is concentrated in vacuo under conditions of being -0.1MPa and eliminates petroleum ether and obtain crude oil；In crude oil
Add the NaHCO that mass fraction is 15%3Solution, and centrifuge 20min, stratification under conditions of speed is 2000r/min
Supernatant is taken to obtain decoloration crude oil；Wherein, NaHCO3The volume ratio of solution and crude oil is 1:200；Decoloration crude oil is crossed 60 mesh to live
Property charcoal post, the impurity such as grease filtered off in decoloration crude oil obtains extract I；Remove layer suspension is according to mass ratio with petroleum ether
1:15 ratio adds petroleum ether, through 8.6m3The flow of/h is filtered by vacuum, and obtains filtrate, and filtrate is placed in temperature for 65
DEG C, the condition that vacuum is -0.1MPa be concentrated in vacuo, obtain oil recovery, then by oil recovery in boiling water bath with 8.4m3The flow of/h
60 mesh activated-charcoal columns are crossed after vacuum suction 20min, up to extract II；Merge extract I and extract II obtains Apple Seeds and carries
Take thing, the Apple Seeds essential oil content in apple seed extract is 354.26mg/g, content of vitamin E 89.36mg/g, vitamin
C content is 59.36mg/g.
The extracting method of propolis extract is in the present embodiment：By propolis and warm water according to solid-liquid mass ratio 1:1 is mixed
Close, soaked 3 times, each 40min in the stirred in water bath temperature that temperature is 50 DEG C, filtering, it is straight that merging filtrate carries out vacuum distillation concentration
To paste, the relative density of paste is 1.32g/cm3The propolis extract is obtained, general flavone content is in extract
214.25mg/g, rutin content 74.65mg/g, myricetin content are 54.36mg/g, Quantitative Determination of Apigenin 49.55mg/g.
The extracting method of malt embryo extract is in the present embodiment：According to plumule and water ratio it is 1 by barley germs:1 adds
Water is ground, and is then placed in 90 DEG C of water-baths and is preheated, and then adds the mass percent with mixed liquor equal quality ratio
For 5% NaHCO3Solution, is then placed in constant temperature blender with magnetic force and shakes, and protein is dissolved in 5% NaHCO3Solution,
2000r/min centrifuges 30min, collects supernatant, obtains albumin；Take the hydrogen for centrifuging lower floor's material with mass percent is 3%
Sodium hydroxide solution mixes, and is then collected supernatant according to same method (centrifugal rotational speed, time are identical), obtains globulin；Again will
Residue adds the ethanol solution that percentage by volume is 75, and dissolving, collects supernatant, obtain protein,alcohol-soluble；Then will be above-mentioned
Albumin, globulin and protein,alcohol-soluble are mixed to get malt embryo extract, and protein wt is in malt embryo extract
684.25mg/g；Content of vitamin E is 106.25mg/g.
In the present embodiment, potato essential oil and percentage by weight that potato composite essential oil is 70% by percentage by weight are
30% auxiliary material essential oil composition, the potato essential oil of the present embodiment are made by potato；Auxiliary material essential oil by following parts by weight into
Divide and be made：17 parts of Chinese toon wood skin, 13 parts of Radix agastaches, 24 parts of Semen sesami nigrum and 34 parts of the leaf of bamboo.
In the present embodiment, potato composite essential oil preparation method includes potato extraction of essential oil, auxiliary material extraction of essential oil and essence
Three steps of oil mixing are made, and comprise the following steps that：
(1) potato extraction of essential oil：
Step S1：Potato is puffing：Potato is cut into the potato block that thickness is 1mm, then potato block is put into temperature
Spend in the frozen water for 0 DEG C and soak 1h, potato block is taken out to drain to be totally submerged in rapeseed oil and is taken out after holding 1s, is put into steaming
Cooked in pot, mashed into paste, it is 9MPa that mashed potato then is put into pressure condition, and temperature is to be kept in 100 DEG C of hot wind
10min, pressure release, then it is 0MPa that mashed potato is put into pressure condition, keeps 5min to complete potato in the cold wind that temperature is 0 DEG C
It is puffing；
Step S2：Cellulase treatment：By potatos of the step S1 after puffing and biology enzyme I solution according to solid-liquid mass ratio
For 1:1 mixing immersion, 20min is stood in the insulating box that temperature is 27 DEG C, is then concentrated, drying goes moisture removal to obtain horse
Bell potato dry powder；Wherein, biology enzyme I solution is made of the raw material of following parts by weight：30 parts of amylase, 20 parts of carbohydrase, 5 parts
Aspergillus oryzae, 10 parts of barbaloin and 250 parts of mass percents be 1% sodium hydroxide solution；The enzyme activity of the amylase
For 800u/g；The enzyme activity of the carbohydrase is 1000u/g；The living bacteria count of the aspergillus oryzae is 3.1 × 107；
Step S3：Microwave treatment：After the potato flour of step S2 is cooled to room temperature, it is laid in plate, tiling is thick
Spend for 1mm, be placed on microwave oven turntable and carry out microwave treatment, the frequency of the microwave treatment is 2300MHZ, and power is
1000W, total duration 180s；
Step S4：Supercritical CO2Extraction：By in the potato flour input extraction kettle after step S3 microwave treatments, pressing
When power is 15-20MPa, with liquid CO2Static extracting 30min, then progressively discharges pressure, carries out supercritical CO2Dynamic extraction,
Dynamic extraction technological parameter is：When temperature is heated to 40 DEG C, pressurize to system, after pressure reaches 40MPa, adjust CO2
Flow is 15L/min, keeps constant temperature and pressure to discharge after carrying out cycling extraction 2h, is centrifuged under conditions of 800r/min
Obtain potato essential oil.
(2) auxiliary material extraction of essential oil：
Step A1：Auxiliary material is puffing：Each raw material is weighed by above-mentioned parts by weight to be crushed respectively, is selected by 30 mesh sieve mesh screens,
Then it is 1 according to solid-liquid mass ratio by ethanol solution that Chinese toon wood skin powder and percentage by volume are 75%:2 mixing；By Radix agastaches
The butanol solution that powder is 10% with percentage by volume is 1 according to solid-liquid mass ratio:1 mixing；By Semen sesami nigrum powder and volume hundred
The ethanol solution that fraction is 75% is 1 according to solid-liquid mass ratio:1 mixing；According to solid-liquid mass ratio it is 1 by leaf of bamboo powder and water:1
Mixing；Then each powder soak is placed in the refrigerator-freezer that temperature is -2 DEG C and refrigerates 24h；Filtering, takes each powder to mix, is put into dish
It is 8MPa that mixed-powder is put into pressure condition after being infiltrated in seed oil, and temperature is that 20min is kept in 110 DEG C of hot wind, pressure release, then
It is 0MPa that mashed potato is put into pressure condition, keeps 15min to complete auxiliary material in the cold wind that temperature is 0 DEG C puffing；
Step A2：Cellulase treatment：By auxiliary materials of the step S1 after puffing and biology enzyme II solution according to solid-liquid mass ratio
For 1:1 mixing immersion, 20min is stood in temperature is 27 DEG C of insulating box, is then concentrated, dry to go moisture removal to obtain auxiliary
Expect dry powder；Wherein, biology enzyme II solution is made of the raw material of following parts by weight：15 parts of pectase, 30 parts of cellulase, 20
The sodium chloride solution that the amylase, 10 parts of aspergillus flavus and 300 parts of mass percents of part are 2%；The enzyme activity of the pectase
For 1200u/g；The enzyme activity of the cellulase is 1500u/g；The enzyme activity of the amylase is 900u/g；The aspergillus flavus
Living bacteria count be 3.9 × 108；
Step A3：Supercritical CO2Extraction：The auxiliary material dry powder of step S2 is put into extraction kettle, when pressure is 15MPa,
With liquid CO2Static extracting 20min, then progressively discharges pressure, carries out supercritical CO2Dynamic extraction, dynamic extraction technological parameter
For：When temperature is heated to 50 DEG C, pressurize to system, after pressure reaches 30MPa, adjust CO2Flow is 15L/min, is protected
Hold after constant temperature and pressure carries out cycling extraction 3h and discharge, be centrifuged to obtain auxiliary material essential oil under conditions of 800r/min.
(3) essential oil mixes：
Potato essential oil and auxiliary material essential oil are weighed according to percentage by weight, is 75% by potato essential oil and percentage by volume
Ethanol solution according to 2:1 is mixed, and 5min is stirred under the speed conditions of 500r/min, then adds auxiliary material essential oil,
Continue to stir 6min, rotary evaporation concentration goes moisture removal to be made.
The preparation method of the present embodiment spot-dispelling liquid includes the following steps：
(1) each material composition is weighed by the parts by weight；
(2) potato composite essential oil is mixed with olive oil, and is put into stirring in homogenizer and emulsion is made, it is described
Mixing time is 25min；Speed of agitator is 700r/min；Then emulsion is put into Ultrasound Instrument again and is ultrasonically treated, surpassed
Sonication power is 800w, sonication treatment time 10min, and it is 50 DEG C to be ultrasonically treated temperature, is cooled to emulsion after room temperature
Temperature is put into be freezed in -7 DEG C of refrigerator-freezers, cooling time is the emulsion after 8h-12h is freezed；
(3) step (2) emulsion is put into the water-bath that temperature is 80 DEG C and heats 30min, then added ginkgo leaf and carry
Take thing, mulberry-leaf extract, apple seed extract and malt embryo extract and be put into homogenizer and be stirred, mixing time
For 10min, speed of agitator 1000r/min；Hyaluronic acid is placed into after stirring and propolis extract is uniformly mixed, and
Speed of agitator is to stir 5min in the mixer of 500r/min；Spot-dispelling liquid crude mixture is obtained, spot-dispelling liquid crude mixture is put into
Sealing ageing 16d is carried out in ceramic bottle, obtains the spot-dispelling liquid.
Embodiment 2：
The present embodiment spot-dispelling liquid comprises the following components in parts by weight：30 parts of potato composite essential oil, 25 parts of ginkgo
Leaf extract, 21 parts of mulberry-leaf extract, 28 parts of apple seed extract, 29 parts of propolis extract, 28 parts of Fructus Hordei Germinatus carry
Take thing, 38 parts of hyaluronic acid and 25 parts of olive oil.
The extracting method of ginkgo biloba p.e in the present embodiment, the extracting method of mulberry-leaf extract, apple seed extract
Extracting method, the extracting method of propolis extract, the extracting method of malt embryo extract and embodiment 1 are completely the same.
Wherein, the potato essential oil and percentage by weight that potato composite essential oil is 90% by percentage by weight are 10%
Auxiliary material essential oil forms, and the potato essential oil of the present embodiment is made by potato；The auxiliary material essential oil of the present embodiment is by following parts by weight
Component be made：29 parts of Chinese toon wood skin, 31 parts of Radix agastaches, 32 parts of Semen sesami nigrum and 46 parts of the leaf of bamboo.
In the present embodiment, potato composite essential oil preparation method includes potato extraction of essential oil, auxiliary material extraction of essential oil and essence
Three steps of oil mixing are made, and comprise the following steps that：
(1) potato extraction of essential oil：
Step S1：Potato is puffing：Potato is cut into the potato block that thickness is 3mm, then potato block is put into temperature
Spend in the frozen water for 2 DEG C and soak 2h, potato block is taken out to drain to be totally submerged in rapeseed oil and is taken out after holding 2s, is put into steaming
Cooked in pot, mashed into paste, it is 10MPa that mashed potato then is put into pressure condition, and temperature is to be kept in 105 DEG C of hot wind
15min, pressure release, then it is 2MPa that mashed potato is put into pressure condition, keeps 5min-10min to complete in the cold wind that temperature is 5 DEG C
Potato is puffing；
Step S2：Cellulase treatment：By potatos of the step S1 after puffing and biology enzyme I solution according to solid-liquid mass ratio
For 1:2 mixing immersions, 30min is stood in the insulating box that temperature is 27 DEG C, is then concentrated, drying goes moisture removal to obtain horse
Bell potato dry powder；Wherein, biology enzyme I solution is made of the raw material of following parts by weight：35 parts of amylase, 25 parts of carbohydrase, 10
The sodium hydroxide solution that the aspergillus oryzae, 20 parts of barbaloin and 300 parts of mass percents of part are 1%；The enzyme activity of the amylase
Power is 1000u/g；The enzyme activity of the carbohydrase is 1200u/g；The living bacteria count of the aspergillus oryzae is 6.7 × 107CFU/
g；
Step S3：Microwave treatment：After the potato flour of step S2 is cooled to room temperature, it is laid in plate, tiling is thick
Spend for 3mm, be placed on microwave oven turntable and carry out microwave treatment, the frequency of the microwave treatment is 2300MHZ, and power is
1000W, total duration 180s；
Step S4：Supercritical CO2Extraction：By in the potato flour input extraction kettle after step S3 microwave treatments, pressing
When power is 20MPa, with liquid CO2Static extracting 40min, then progressively discharges pressure, carries out supercritical CO2Dynamic extraction, dynamic
Extraction process parameter is：When temperature is heated to 50 DEG C, pressurize to system, after pressure reaches 50MPa, adjust CO2Flow
For 25L/min, keep constant temperature and pressure to discharge after carrying out cycling extraction 4h, be centrifuged under conditions of 1000r/min
To potato essential oil.
(2) auxiliary material extraction of essential oil：
Step A1：Auxiliary material is puffing：Each raw material is weighed by above-mentioned parts by weight to be crushed respectively, is selected by 50 mesh sieve mesh screens,
Then it is 1 according to solid-liquid mass ratio by ethanol solution that Chinese toon wood skin powder and percentage by volume are 75%:2 mixing；By Radix agastaches
The butanol solution that powder is 10% with percentage by volume is 1 according to solid-liquid mass ratio:1 mixing；By Semen sesami nigrum powder and volume hundred
The ethanol solution that fraction is 75% is 1 according to solid-liquid mass ratio:1 mixing；According to solid-liquid mass ratio it is 1 by leaf of bamboo powder and water:1
Mixing；Then each powder soak is placed in the refrigerator-freezer that temperature is 0 DEG C and refrigerates 26h；Filtering, takes each powder to mix, is put into dish
It is 9MPa that mixed-powder is put into pressure condition after being infiltrated in seed oil, and temperature is that 25min is kept in 120 DEG C of hot wind, pressure release, then
It is 2MPa that mashed potato is put into pressure condition, keeps 20min to complete auxiliary material in the cold wind that temperature is 5 DEG C puffing；
Step A2：Cellulase treatment：By auxiliary materials of the step S1 after puffing and biology enzyme II solution according to solid-liquid mass ratio
For 1:2 mixing immersions, 30min is stood in temperature is 27 DEG C of insulating box, is then concentrated, dry to go moisture removal to obtain auxiliary
Expect dry powder；Wherein, biology enzyme II solution is made of the raw material of following parts by weight：25 parts of pectase, 50 parts of cellulase, 30
The sodium chloride solution that the amylase, 20 parts of aspergillus flavus and 350 parts of mass percents of part are 2%；The enzyme activity of the pectase
For 1500u/g；The enzyme activity of the cellulase is 2000u/g；The enzyme activity of the amylase is 1200u/g；It is described yellow bent
Mould living bacteria count is 6.7 × 108CFU/g；
Step A3：Supercritical CO2Extraction：The auxiliary material dry powder of step S2 is put into extraction kettle, when pressure is 20MPa,
With liquid CO2Static extracting 30min, then progressively discharges pressure, carries out supercritical CO2Dynamic extraction, dynamic extraction technological parameter
For：When temperature is heated to 60 DEG C, pressurize to system, after pressure reaches 40MPa, adjust CO2Flow is 25L/min, is protected
Hold after constant temperature and pressure carries out cycling extraction 5h and discharge, be centrifuged to obtain auxiliary material essential oil under conditions of 1000r/min.
(3) essential oil mixes：
Potato essential oil and auxiliary material essential oil are weighed according to percentage by weight, is 75% by potato essential oil and percentage by volume
Ethanol solution according to 2:1 is mixed, and 7min is stirred under the speed conditions of 800r/min, then adds auxiliary material essential oil,
Continue to stir 8min, rotary evaporation concentration goes moisture removal to be made.
The preparation method of the present embodiment spot-dispelling liquid includes the following steps：
(1) each material composition is weighed by the parts by weight；
(2) potato composite essential oil is mixed with olive oil, and is put into stirring in homogenizer and emulsion is made, it is described
Mixing time is 35min；Speed of agitator is 900r/min；Then emulsion is put into Ultrasound Instrument again and is ultrasonically treated, surpassed
Sonication power is 1000w, sonication treatment time 20min, and it is 60 DEG C to be ultrasonically treated temperature, is cooled to emulsion after room temperature
Being put into temperature is -- freezed in 5 DEG C of refrigerator-freezers, cooling time is the emulsion after 12h is freezed；
(3) step (2) emulsion is put into water-bath at a temperature of 90 °C and heats 40min, then added ginkgo leaf and carry
Take thing, mulberry-leaf extract, apple seed extract and malt embryo extract and be put into homogenizer and be stirred, mixing time
For 15min, speed of agitator 1200r/min；Hyaluronic acid is placed into after stirring and propolis extract is uniformly mixed, and
Speed of agitator is to stir 10min in the mixer of 700r/min；Spot-dispelling liquid crude mixture is obtained, spot-dispelling liquid crude mixture is put into
Sealing ageing 22d is carried out in ceramic bottle, obtains the spot-dispelling liquid.
Embodiment 3：
The present embodiment spot-dispelling liquid comprises the following components in parts by weight：25 parts of potato composite essential oil, 20 parts of ginkgo
Leaf extract, 18 parts of mulberry-leaf extract, 19 parts of apple seed extract, 22 parts of propolis extract, 21 parts of Fructus Hordei Germinatus carry
Take thing, 29 parts of hyaluronic acid and 20 parts of olive oil.
The extracting method of ginkgo biloba p.e in the present embodiment, the extracting method of mulberry-leaf extract, apple seed extract
Extracting method, the extracting method of propolis extract, the extracting method of malt embryo extract and embodiment 1 are completely the same.
Wherein, the potato essential oil and percentage by weight that potato composite essential oil is 80% by percentage by weight are 20%
Auxiliary material essential oil forms, and the potato essential oil of the present embodiment is made by potato；The auxiliary material essential oil of the present embodiment is by following parts by weight
Component be made：21 parts of Chinese toon wood skin, 21 parts of Radix agastaches, 24 parts of Semen sesami nigrum and 40 parts of the leaf of bamboo.
In the present embodiment, potato composite essential oil preparation method includes potato extraction of essential oil, auxiliary material extraction of essential oil and essence
Three steps of oil mixing are made, and comprise the following steps that：
(1) potato extraction of essential oil：
Step S1：Potato is puffing：Potato is cut into the potato block that thickness is 2mm, then potato block is put into temperature
Spend in the frozen water for 1 DEG C and soak 1.5h, potato block is taken out to drain to be totally submerged in rapeseed oil and is taken out after holding 2s, is put into
Cooked in steamer, mashed into paste, it is 9MPa that mashed potato then is put into pressure condition, and temperature is to be kept in 103 DEG C of hot wind
12min, pressure release, then it is 1MPa that mashed potato is put into pressure condition, keeps 8min to complete potato in the cold wind that temperature is 2 DEG C
It is puffing；
Step S2：Cellulase treatment：By potatos of the step S1 after puffing and biology enzyme I solution according to solid-liquid mass ratio
For 1:2 mixing immersions, 25min is stood in the insulating box that temperature is 27 DEG C, is then concentrated, drying goes moisture removal to obtain horse
Bell potato dry powder；Wherein, biology enzyme I solution is made of the raw material of following parts by weight：32 parts of amylase, 22 parts of carbohydrase, 8 parts
Aspergillus oryzae, 15 parts of barbaloin and 270 parts of mass percents be 1% sodium hydroxide solution；The enzyme activity of the amylase
For 900u/g；The enzyme activity of the carbohydrase is 1100u/g；The living bacteria count of the aspergillus oryzae is 4.7 × 107CFU/g；
Step S3：Microwave treatment：After the potato flour of step S2 is cooled to room temperature, it is laid in plate, tiling is thick
Spend for 2mm, be placed on microwave oven turntable and carry out microwave treatment, the frequency of the microwave treatment is 2300MHZ, and power is
1000W, total duration 180s；
Step S4：Supercritical CO2Extraction：By in the potato flour input extraction kettle after step S3 microwave treatments, pressing
When power is 18MPa, with liquid CO2Static extracting 35min, then progressively discharges pressure, carries out supercritical CO2Dynamic extraction, dynamic
Extraction process parameter is：When temperature is heated to 45 DEG C, pressurize to system, after pressure reaches 45MPa, adjust CO2Flow
For 20L/min, keep constant temperature and pressure to discharge after carrying out cycling extraction 3h, be centrifuged to obtain under conditions of 900r/min
Potato essential oil.
(2) auxiliary material extraction of essential oil：
Step A1：Auxiliary material is puffing：Each raw material is weighed by above-mentioned parts by weight to be crushed respectively, is selected by 40 mesh sieve mesh screens,
Then it is 1 according to solid-liquid mass ratio by ethanol solution that Chinese toon wood skin powder and percentage by volume are 75%:2 mixing；By Radix agastaches
The butanol solution that powder is 10% with percentage by volume is 1 according to solid-liquid mass ratio:1 mixing；By Semen sesami nigrum powder and volume hundred
The ethanol solution that fraction is 75% is 1 according to solid-liquid mass ratio:1 mixing；According to solid-liquid mass ratio it is 1 by leaf of bamboo powder and water:1
Mixing；Then each powder soak is placed in the refrigerator-freezer that temperature is -1 DEG C and refrigerates 25h；Filtering, takes each powder to mix, is put into dish
It is 9MPa that mixed-powder is put into pressure condition after being infiltrated in seed oil, and temperature is that 23min is kept in 115 DEG C of hot wind, pressure release, then
It is 1MPa that mashed potato is put into pressure condition, keeps 18min to complete auxiliary material in the cold wind that temperature is 3 DEG C puffing；
Step A2：Cellulase treatment：By auxiliary materials of the step S1 after puffing and biology enzyme II solution according to solid-liquid mass ratio
For 1:1 mixing immersion, 25min is stood in temperature is 27 DEG C of insulating box, is then concentrated, dry to go moisture removal to obtain auxiliary
Expect dry powder；Wherein, biology enzyme II solution is made of the raw material of following parts by weight：20 parts of pectase, 40 parts of cellulase, 25
The sodium chloride solution that the amylase, 15 parts of aspergillus flavus and 330 parts of mass percents of part are 2%；The enzyme activity of the pectase
For 1300u/g；The enzyme activity of the cellulase is 1800u/g；The enzyme activity of the amylase is 1100u/g；It is described yellow bent
Mould living bacteria count is 5.7 × 108CFU/g；
Step A3：Supercritical CO2Extraction：The auxiliary material dry powder of step S2 is put into extraction kettle, when pressure is 17MPa,
With liquid CO2Static extracting 25min, then progressively discharges pressure, carries out supercritical CO2Dynamic extraction, dynamic extraction technological parameter
For：When temperature is heated to 55 DEG C, pressurize to system, after pressure reaches 35MPa, adjust CO2Flow is 20L/min, is protected
Hold after constant temperature and pressure carries out cycling extraction 4h and discharge, be centrifuged to obtain auxiliary material essential oil under conditions of 900r/min.
(3) essential oil mixes：
Potato essential oil and auxiliary material essential oil are weighed according to percentage by weight, is 75% by potato essential oil and percentage by volume
Ethanol solution according to 2:1 is mixed, and 6min is stirred under the speed conditions of 700r/min, then adds auxiliary material essential oil,
Continue to stir 7min, rotary evaporation concentration goes moisture removal to be made.
The preparation method of the present embodiment spot-dispelling liquid includes the following steps：
(1) each material composition is weighed by the parts by weight；
(2) potato composite essential oil is mixed with olive oil, and is put into stirring in homogenizer and emulsion is made, it is described
Mixing time is 30min；Speed of agitator is 800r/min；Then emulsion is put into Ultrasound Instrument again and is ultrasonically treated, surpassed
Sonication power is 900w, sonication treatment time 15min, and it is 55 DEG C to be ultrasonically treated temperature, is cooled to emulsion after room temperature
Temperature is put into be freezed in -6 DEG C of refrigerator-freezers, cooling time is the emulsion after 10h is freezed；
(3) step (2) emulsion is put into the water-bath that temperature is 85 DEG C and heats 35min, then added ginkgo leaf and carry
Take thing, mulberry-leaf extract, apple seed extract and malt embryo extract and be put into homogenizer and be stirred, mixing time
For 12min, speed of agitator 1100r/min；Hyaluronic acid is placed into after stirring and propolis extract is uniformly mixed, and
Speed of agitator is to stir 8min in the mixer of 600r/min；Spot-dispelling liquid crude mixture is obtained, spot-dispelling liquid crude mixture is put into
Sealing ageing 20d is carried out in ceramic bottle, obtains the spot-dispelling liquid.
(1) the research control experiment of potato composite essential oil characteristic：
Control group 1：
The potato composite essential oil of this control group contains only potato essential oil, without using auxiliary material essential oil, other preparation methods with
Embodiment 1 is identical.
Control group 2：
The potato composite essential oil of this control group contains only auxiliary material essential oil, without potato essential oil, other preparation methods and reality
It is identical to apply example 1.
Control group 3：
The auxiliary material essential oil of this control group potato composite essential oil only contains Radix agastaches, Semen sesami nigrum and several components of the leaf of bamboo, i.e.,
The auxiliary material essence that the potato essential oil and percentage by weight that the potato essential oil of this control group is 70% by percentage by weight are 30%
Oil composition, wherein, potato essential oil is made by potato；Auxiliary material essential oil is made by the component of following parts by weight：13 parts of wrinkled giant hyssop
Leaf, 24 parts of Semen sesami nigrum and 34 parts of leaf of bamboo composition, other preparation methods are identical with embodiment 1.
Control group 4：
The auxiliary material essential oil of this control group potato composite essential oil only contains Chinese toon wood skin, Semen sesami nigrum and several components of the leaf of bamboo, i.e.,
The auxiliary material essence that the potato essential oil and percentage by weight that the potato essential oil of this control group is 70% by percentage by weight are 30%
Oil composition, wherein, potato essential oil is made by potato；Auxiliary material essential oil is made by the component of following parts by weight：17 parts of Chinese toon wood
Skin, 24 parts of Semen sesami nigrum and 34 parts of leaf of bamboo composition, other preparation methods are identical with embodiment 1.
Control group 5：
The auxiliary material essential oil of this control group potato composite essential oil only contains Chinese toon wood skin, Radix agastaches and several components of the leaf of bamboo, i.e.,
The auxiliary material essence that the potato essential oil and percentage by weight that the potato essential oil of this control group is 70% by percentage by weight are 30%
Oil composition, wherein, potato essential oil is made by potato；Auxiliary material essential oil is made by the component of following parts by weight：17 parts of Chinese toon wood
Skin, 13 parts of Radix agastaches and 34 parts of leaf of bamboo composition, other preparation methods are identical with embodiment 1.
Control group 6：
The auxiliary material essential oil of this control group potato composite essential oil only contains Chinese toon wood skin, Radix agastaches and several components of Semen sesami nigrum,
That is the auxiliary material that the potato essential oil and percentage by weight that the potato essential oil of this control group is 70% by percentage by weight are 30%
Essential oil forms, wherein, potato essential oil is made by potato；Auxiliary material essential oil is made by the component of following parts by weight：17 parts of Chinese toon
Wood skin, 13 parts of Radix agastaches and 24 parts of Semen sesami nigrum composition, other preparation methods are identical with embodiment 1.
Control group 7：
Without expanding treatment in the potato essential oil producing process of this control group potato composite essential oil, but directly will
Potato flour is digested, supercritical CO2Extraction, other preparation methods are identical with embodiment 1.
Control group 8：
Without enzymolysis in the potato essential oil producing process of this control group potato composite essential oil, but carry out potato
Puffing, supercritical CO2Extraction, other preparation methods are identical with embodiment 1.
Control group 9：
Only pass through supercritical CO in the potato essential oil producing process of this control group potato composite essential oil2Extraction, it is other
Preparation method is identical with embodiment 1.
Control group 10：
Without expanding treatment in the auxiliary material essential oil producing process of this control group potato composite essential oil, but directly will be auxiliary
Feed powder end is digested, supercritical CO2Extraction, other preparation methods are identical with embodiment 1.
Control group 11：
Without enzymolysis in the auxiliary material essential oil producing process of this control group potato composite essential oil, but carry out auxiliary material powder
Through puffing, supercritical CO2Extraction, other preparation methods are identical with embodiment 1.
Control group 12：
Only pass through supercritical CO in the auxiliary material essential oil producing process of this control group potato composite essential oil2Extraction, other systems
Preparation Method is identical with embodiment 1.
Test experiments 1：
Measure in embodiment 1-3 and control group 1-12, the yield of the potato composite essential oil of preparation, Vitamin C content with
And DPPH radical scavenging activities, concrete condition are shown in Table 1：
Table 1
As seen from the above table, the refined oil rate of embodiment 1-3 is suitable with control group 1-6 higher than control group 7-12, illustrates this Shen
Please puffing, biological enzymolysis, supercritical CO in method of extraction of essential oil2Extraction phase combination can effectively improve refined oil rate：Embodiment 1-3
In ascorbic content apparently higher than control group 1, illustrate to contain more rich vitamin C component in potato essential oil；Embodiment
The Vitamin C content of 1-3 apparently higher than control group 7-9 and control group 10-12, illustrate in potato method of extraction of essential oil it is puffing,
Biological enzymolysis, supercritical CO2Extraction phase combination can effectively improve Vitamin C content；The DPPH free radical scavenging activities of embodiment 1-3
Apparently higher than control group 1-12, illustrate that the essential oil of the application and method of extraction of essential oil can effectively remove the raising of DPPH free radicals
The antioxygen rate of essential oil.
Test experiments 2：
Measure in embodiment 1 and control group 1-12, the oily substance component content of potato composite essential oil, concrete condition is shown in
Table 2：
Table 2
As seen from the above table, the ester type compound component of embodiment 1 is above control group 1-12, and ketone compounds component is low
In control group 1-12, illustrate, the ester-containing amount of potato essential oil of the application is high, and amount containing ketone is low, illustrates the potato essential oil of the application
Its fragrance does not have other control groups to be pungent.
(2) spot-dispelling liquid characteristic test is tested：
The present invention calculates rejection ability of the spot-dispelling liquid to tyrosinase activity using the method for measure tyrosinase activity,
The spot-dispelling liquid of following test group and distilled water are mixed, spot-dispelling liquid is configured to the nti-freckle solution of 5mg/ml, then takes test group
Each 30 μ l of nti-freckle solution mixed with the tyrosine enzyme liquid of 10 μ l, 15min is kept the temperature at 30 DEG C, then measure work as tyrosine enzyme activity
Concentration (the IC of nti-freckle solution during power drop by half50)；After above-mentioned spot-dispelling liquid is irradiated 3h under sunlight irradiation, then measure to body
The IC of extracellular tyrosinase inhibition of enzyme activity rate50Value, concrete condition are shown in Table 3：
Test group 1：Spot-dispelling liquid is produced according to the method for embodiment 1；
Test group 2：Spot-dispelling liquid is configured according to following component：20 parts of the potato composite essential oil (potato of this test group
Composite essential oil is free of auxiliary material derived essential oil, is only made of potato essential oil), 15 parts of ginkgo biloba p.e, 11 parts of mulberry leaf carry
Take thing, 13 parts of apple seed extract, 17 parts of propolis extract, 13 parts of malt embryo extract, 25 parts of hyaluronic acid and
14 parts of olive oil.Other preparation methods and embodiment 1 are completely the same.
Test group 3：Spot-dispelling liquid is configured according to following component：15 parts of ginkgo biloba p.e, 11 parts of mulberry-leaf extract, 13
The apple seed extract, 17 parts of propolis extract, 13 parts of malt embryo extract, 25 parts of hyaluronic acid and 14 parts of olive of part
Olive oil.Other preparation methods and embodiment 1 are completely the same.
Test group 4：Spot-dispelling liquid is configured according to following component：20 parts of potato composite essential oil, 11 parts of mulberry-leaf extract,
13 parts of apple seed extract, 17 parts of propolis extract, 13 parts of malt embryo extract, 25 parts of hyaluronic acid and 14 parts
Olive oil.Other preparation methods and embodiment 1 are completely the same.
Test group 5：Spot-dispelling liquid is configured according to following component：20 parts of potato composite essential oil, the extraction of 15 parts of ginkgo leaf
Thing, 13 parts of apple seed extract, 17 parts of propolis extract, 13 parts of malt embryo extract, 25 parts of hyaluronic acid and 14
The olive oil of part.Other preparation methods and embodiment 1 are completely the same.
Test group 6：Spot-dispelling liquid is configured according to following component：20 parts of potato composite essential oil, the extraction of 15 parts of ginkgo leaf
Thing, 11 parts of mulberry-leaf extract, 17 parts of propolis extract, 13 parts of malt embryo extract, 25 parts of hyaluronic acid and 14 parts
Olive oil.Other preparation methods and embodiment 1 are completely the same.
Test group 7：Spot-dispelling liquid is configured according to following component：20 parts of potato composite essential oil, the extraction of 15 parts of ginkgo leaf
Thing, 11 parts of mulberry-leaf extract, 13 parts of apple seed extract, 13 parts of malt embryo extract, 25 parts of hyaluronic acid and 14
The olive oil of part.Other preparation methods and embodiment 1 are completely the same.
Test group 8：Spot-dispelling liquid is configured according to following component：20 parts of potato composite essential oil, the extraction of 15 parts of ginkgo leaf
Thing, 11 parts of mulberry-leaf extract, 13 parts of apple seed extract, 17 parts of propolis extract, 25 parts of hyaluronic acid and 14 parts
Olive oil.Other preparation methods and embodiment 1 are completely the same.
Test group 9：Spot-dispelling liquid is configured according to following component：20 parts of potato composite essential oil, the extraction of 15 parts of ginkgo leaf
Thing, 11 parts of mulberry-leaf extract, 13 parts of apple seed extract, 17 parts of propolis extract, 13 parts of malt embryo extract and
14 parts of olive oil.Other preparation methods and embodiment 1 are completely the same.
Test group 10：Spot-dispelling liquid is configured according to following component：20 parts of potato composite essential oil, the extraction of 15 parts of ginkgo leaf
Thing, 11 parts of mulberry-leaf extract, 13 parts of apple seed extract, 17 parts of propolis extract, 13 parts of malt embryo extract and
25 parts of hyaluronic acid.Other preparation methods and embodiment 1 are completely the same.
Control group：Water is used without using any spot-dispelling liquid.
Table 3
As seen from the above table, the IC of test group 150Value is significantly lower than test group 2-9, more below control group, illustrates the application's
Potato composite essential oil, ginkgo biloba p.e, mulberry-leaf extract, apple seed extract, propolis extract, malt embryo extract,
Hyaluronic acid and olive oil compounding use have good suppression tyrosinase activity effect；There is good nti-freckle ability, moreover,
The forward and backward IC of the illumination of test group 1,250It is worth basically identical, and the IC after test group 3-9 illumination50Before value is significantly greater than illumination,
Illustrate, to illumination-insensitive after the composite essential oil compounding of the application, and the component of the application lacks one and can all cause spot-dispelling liquid to exist
It is decomposed under illumination, causes suppression tyrosinase activity poor, influence freckle effect.
In conclusion refined oil rate, raising essence can be effectively improved by preparing potato composite essential oil using the method for the present invention
Oily oxidation resistance, and its fragrance is more comfortable；Meanwhile there is strong anti-oxidation using potato composite essential oil in spot-dispelling liquid,
Whitening function can be played, is extracted with ginkgo biloba p.e, mulberry-leaf extract, apple seed extract, propolis extract, Fructus Hordei Germinatus
After thing, hyaluronic acid and olive oil compounding, the inoxidizability of spot-dispelling liquid can be effectively improved, improves the stability of potato essential oil,
The light sensitivity of potato essential oil is reduced, improves freckle effect.
Embodiment described above only expresses the several embodiments of the present invention, its description is more specific and detailed, but simultaneously
Therefore limitation of the scope of the invention cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art,
Without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection model of the present invention
Enclose.Therefore, protection scope of the present invention should be determined by the appended claims.
Claims (7)
1. a kind of spot-dispelling liquid, it is characterised in that the spot-dispelling liquid comprises the following components in parts by weight：20 parts -30 parts of Ma Ling
Potato composite essential oil, 15 parts -25 parts of ginkgo biloba p.e, 11 parts -21 parts of mulberry-leaf extract, 13 parts -28 parts of Apple Seeds carry
Take thing, 17 parts -29 parts of propolis extract, 13 parts -28 parts of malt embryo extract, 25 parts -38 parts of hyaluronic acid and 14 parts -
25 parts of olive oil.
2. spot-dispelling liquid according to claim 1, it is characterised in that the potato composite essential oil is by percentage by weight
The auxiliary material essential oil that the potato essential oil and percentage by weight of 70%-90% is 10%-30% forms.
3. spot-dispelling liquid according to claim 2, it is characterised in that the potato essential oil is made by potato；It is described auxiliary
Material essential oil is made by the component of following parts by weight：17 parts -29 parts of Chinese toon wood skin, 13 parts -31 parts of Radix agastaches, 24 parts -32 parts
Semen sesami nigrum and 34 parts -46 parts of the leaf of bamboo.
4. spot-dispelling liquid according to claim 2, it is characterised in that the potato composite essential oil preparation method includes Ma Ling
Potato extraction of essential oil, auxiliary material extraction of essential oil and essential oil mix three steps and are made.
5. spot-dispelling liquid according to claim 4, it is characterised in that the potato extraction of essential oil by potato it is puffing,
Cellulase treatment, microwave treatment and supercritical CO2Four steps of extraction obtain；The biology enzyme I that the cellulase treatment uses is molten
Liquid is made of the raw material of following parts by weight：30 parts -35 parts of amylase, 20 parts -25 parts of carbohydrase, 5 parts -10 parts of meter Qu
The sodium hydroxide solution that mould, 10 parts -20 parts of barbaloin and 250 parts of -300 parts of mass percents are 1%；The enzyme of the amylase
Vigor is 800u/g-1000u/g；The enzyme activity of the carbohydrase is 1000u/g-1200u/g；Effective viable bacteria of the aspergillus oryzae
Number is 3.1 × 107~6.7 × 107CFU/g。
6. spot-dispelling liquid according to claim 4, it is characterised in that the auxiliary material extraction of essential oil is puffing, biological by auxiliary material
Enzymatic treatment and supercritical CO2Four steps of extraction obtain；The biology enzyme II solution that the cellulase treatment uses is by following weight
The raw material of part is made：15 parts -25 parts of pectase, 30 parts -50 parts of cellulase, 20 parts -30 parts of amylase, 10 part -20
The sodium chloride solution that the aspergillus flavus of part and 300 parts of -350 parts of mass percents are 2%；The enzyme activity of the pectase is 1200u/
g-1500u/g；The enzyme activity of the cellulase is 1500u/g-2000u/g；The enzyme activity of the amylase is 900u/g-
1200u/g；The living bacteria count of the aspergillus flavus is 3.9 × 108~6.7 × 108CFU/g。
A kind of 7. preparation method for preparing any one spot-dispelling liquid as described in claim 1-6, it is characterised in that the method bag
Include following steps：
(1) each material composition is weighed by the parts by weight；
(2) potato composite essential oil is mixed with olive oil, and is put into stirring in homogenizer and emulsion, the stirring is made
Time is 25min-35min；Speed of agitator is 700r/min-900r/min；Then emulsion is put into Ultrasound Instrument again and carried out
It is ultrasonically treated, sonification power 800w-1000w, sonication treatment time 10min-20min, it is 50 to be ultrasonically treated temperature
DEG C~60 DEG C, it is cooled to after room temperature and emulsion is put into temperature to be freezed in -7 DEG C~-5 DEG C refrigerator-freezers, cooling time 8h-
12h freezed after emulsion；
(3) step (2) emulsion is put into the water-bath that temperature is 80 DEG C~90 DEG C and heats 30min-40min, then added
Ginkgo biloba p.e, mulberry-leaf extract, apple seed extract and malt embryo extract and being put into homogenizer is stirred,
Mixing time is 10min-15min, speed of agitator 1000r/min-1200r/min；Hyaluronic acid is placed into after stirring
It is uniformly mixed with propolis extract, and 5min-10min is stirred in the mixer that speed of agitator is 500r/min-700r/min；
Spot-dispelling liquid crude mixture is obtained, spot-dispelling liquid crude mixture is put into ceramic bottle and carries out sealing ageing 16d-22d, obtains described dispel
Spot liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711336065.3A CN107898670A (en) | 2017-12-14 | 2017-12-14 | A kind of spot-dispelling liquid and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711336065.3A CN107898670A (en) | 2017-12-14 | 2017-12-14 | A kind of spot-dispelling liquid and preparation method thereof |
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| CN107898670A true CN107898670A (en) | 2018-04-13 |
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ID=61865627
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPWO2021085496A1 (en) * | 2019-11-01 | 2021-05-06 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104922037A (en) * | 2015-06-28 | 2015-09-23 | 青岛宇星智能科技开发有限公司 | Anti-ageing potato facial mask |
| CN104971025A (en) * | 2015-06-28 | 2015-10-14 | 青岛宇星智能科技开发有限公司 | Essence with effects of skin whitening and freckle diluting |
-
2017
- 2017-12-14 CN CN201711336065.3A patent/CN107898670A/en not_active Withdrawn
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104922037A (en) * | 2015-06-28 | 2015-09-23 | 青岛宇星智能科技开发有限公司 | Anti-ageing potato facial mask |
| CN104971025A (en) * | 2015-06-28 | 2015-10-14 | 青岛宇星智能科技开发有限公司 | Essence with effects of skin whitening and freckle diluting |
Non-Patent Citations (1)
| Title |
|---|
| ZINNAL A等: "Extraction kinetics of natural antioxidants from potato industry by-products", 《RESEARCHGATE》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPWO2021085496A1 (en) * | 2019-11-01 | 2021-05-06 | ||
| JP7437055B2 (en) | 2019-11-01 | 2024-02-22 | 株式会社山田養蜂場本社 | Composition, antioxidant, anti-glycation agent, neurite outgrowth promoter and cognitive function improving agent |
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Application publication date: 20180413 |