CN107875453A - Carry preparation method of Types of Medicine electrostatic spinning guide tissue regeneration film and products thereof and application - Google Patents
Carry preparation method of Types of Medicine electrostatic spinning guide tissue regeneration film and products thereof and application Download PDFInfo
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- CN107875453A CN107875453A CN201711098475.9A CN201711098475A CN107875453A CN 107875453 A CN107875453 A CN 107875453A CN 201711098475 A CN201711098475 A CN 201711098475A CN 107875453 A CN107875453 A CN 107875453A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/02—Inorganic materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/222—Gelatin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0015—Electro-spinning characterised by the initial state of the material
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- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
- D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
- D04H1/728—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/252—Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/02—Materials or treatment for tissue regeneration for reconstruction of bones; weight-bearing implants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/12—Materials or treatment for tissue regeneration for dental implants or prostheses
Abstract
The present invention relates to preparation method for carrying Types of Medicine electrostatic spinning guide tissue regeneration film and products thereof and application, including carry medicine HNTs preparation and the preparation of Static Spinning medicament-carrying composite nano-fiber membrane.The present invention loads cancer therapy drug, the phenomenon of burst release that simple medicine can be avoided to occur with high polymer mixing Static Spinning using the HNTs with hollow structure as pharmaceutical carrier so that the multiple guide tissue regeneration film of the Static Spinning of preparation has good therapeutic effect；The inorganic HNTs of doping can improve the mechanical property of composite cellulosic membrane in Static Spinning polymer nanofiber of the present invention, and with good biocompatibility, degradability and promote bone uptake etc., be advantageous to medicament-carrying nano-fiber and be applied to environment inside complexity as functional organizations engineering scaffold material；It is green and raw material HNTs is a kind of cheap natural nano pipe of well-crystallized, and guide tissue regeneration film preparation method technique is simple, it is workable, it further can meet to produce and apply.
Description
Technical field
The present invention relates to a kind of preparation method for carrying Types of Medicine electrostatic spinning guide tissue regeneration film and products thereof and application.Tool
Body is using the poly- of tool degradability and good biocompatibility（Racemic lactic acid-co- caprolactones）With gelatin as matrix material
Material, using galapectite as pharmaceutical carrier, a kind of composite nano-fiber membrane with antibacterial effect is prepared by electrostatic spinning technique.This
Invention belongs to nano biological medical material field.
Background technology
The problem of periodontium and bone tissue defect are common in clinic, guided tissue regeneration are that treatment Cranial defect is most general
Time one of means, improve the performance of guide tissue regeneration film, prepare new guide tissue regeneration film and periodontal and bone are repaiied
Multiple regeneration is of great importance.The preparation of guide tissue regeneration (Guided Tissue Regeneration, GTR) membrane material is group
Knit one of core technology of engineering research.Cell has preferably adhesion special on the fiber of the diameter (nanoscale) less than itself
Property, therefore, the porous three-dimensional cytoskeleton prepared using electrostatic spinning is capable of the structure of preferably bionical natural extracellular matrix
Feature, it is expected to turn into preferable guide tissue regeneration film material.
Macromolecule with biocompatibility particularly natural polymer such as collagen, chitosan, fibroin albumen etc. are by quiet
Fibrofelt obtained by Electrospun has the characteristics that porosity is high, aperture is adjustable, structure-biological compatibility is good, in guiding tissue again
Filming Material Field has broad application prospects.Gelatin（GE）It is to be prepared by the collagen hydrolysate in animal body, has
Good biocompatibility, degradability and low immunogenicity.But its degradation speed is fast, early stage is easily caused to be collapsed in vivo
Fall into, mechanical property deficiency.So in order to improve its mechanical property and degradation speed, typically using compound other polymers.It is poly-（Disappear
Revolve lactic acid-co- caprolactones）（P(DLLA-co-CL)）It is a kind of there is good biocompatibility and biodegradable high score
Sub- polymer, its electrospinning fibre support can be applied to intravascular tissue engineering field, have good toughness.
Halloysite nanotubes (HNTs) are a kind of middle empty nanotubes of natural aluminosilicate hydrochlorate, cheap, are had necessarily
The features such as draw ratio, big natural nano space and inner chamber load volume.Due to its stable structure, excellent mechanical property, heat
Stability and biocompatibility, it is widely used at present among the developmental research of high polymer composite nano material.For passing
The polymer nanofiber mechanical strength of system is low, the pharmaceutical dosage form such as common nano particle, liposome, micella and some electrostatic
Spinning nano fibre carries pharmaceutically dosage form and phenomenon of burst release be present, and the problems such as nanotube medicine-carried system non-device, the present invention is by adopting
The method being combined after carrying medicine with nanotube with electrostatic spinning technique, is prepared for medicament-carried nano pipe/polymer composite fiber film,
So as to realize the release of permanent insoluble drug release and high drug concentration, novel antibacterial guide tissue regeneration film material can be used as.
The content of the invention
For overcome the deficiencies in the prior art, the present invention relates to a kind of preparation for carrying Types of Medicine electrostatic spinning guide tissue regeneration film
Method and products thereof and application.Galapectite load antimicrobial DP finish polymyxins b is used first（PMB）（PMB/HNTs）, then will
PMB/HNTs, GE, P (DLLA-co-CL) are dissolved in hexafluoroisopropanol with certain proportioning（HFIP）In, pass through electrostatic spinning skill
Art equipment is prepared PMB/HNTs/GE/ P (DLLA-co-CL) load medicines under certain voltage, reception distance, solution flow rate and answered
Close nano fibrous membrane.The hybrid composite nano fiber system has among organizational project and regenerative medicine field
It is widely applied prospect.
For purpose as realization, in the inventive solutions, one kind carries Types of Medicine electrostatic spinning guide tissue regeneration
The preparation method of film, with natural nano pipe galapectite（HNTs）For raw material, contained medicine is polymyxins b（PMB）, including it is following
Step：
（1）Carry medicine HNTs preparation
Take a certain amount of addition PMB to be dissolved in pure water and stir 20min, add a certain amount of HNTs solution, ultrasonic disperse afterwards
40min so that galapectite（HNTs）In the fully dispersed solution to PMB；PMB and HNTs mixed solutions are placed in vacuum drying chamber,
Vacuumized under conditions of 0.05, up to liquid surface, there is no bubble appearance；After vacuum drying chamber is taken out, after stirring 5min
It is repeated once and vacuumizes, drive away the bubble of residual in HNTs internal cavities, makes the more PMB inside entered；In 3000rpm
Under conditions of centrifugation take out supernatant it is stand-by, clean precipitation with ultra-pure water, will load medicinal powder end under conditions of 50 DEG C drying 24h,
It is stand-by with using screen filtration after agate mortar mill-drying powder again；
（2）The preparation of Static Spinning medicament-carrying composite nano-fiber membrane
PMB/the HNTs that will necessarily match, gather（Racemic lactic acid-co- caprolactones）（P(DLLA-co-CL)）And gelatin（GE）Add
Hexafluoroisopropanol（HFIP）In, be made into mass concentration 8-10% solution, stir at room temperature obtain after 6 ~ 12h one it is homogeneous molten
Liquid, resulting solution is loaded in a 10 mL glass syringes, in the KV of spinning voltage 10 ~ 13, spinning receive distance for 12 ~
15 cm, under the conditions of solution flow rate is 0.4 ~ 0.5 mL/h, PMB/HNTs/ gelatin/P is obtained by electrospinning device
(DLLA-co-CL) composite nano-fiber membrane, gained film is put into vacuum drying oven after spinning be dried overnight at room temperature it is standby
With.
Described galapectite is 100-300nm.
Described medicine is antimicrobial DP finish polymyxins b.
The present invention provides a kind of load Types of Medicine electrostatic spinning guide tissue regeneration film, is prepared into according to any of the above-described methods described
Arrive, the inorganic doping material part of guide tissue regeneration film is galapectite, and organic material part is poly-（Racemic lactic acid-co- is in oneself
Ester）（P(DLLA-co-CL)）And gelatin（GE）.
The present invention provides a kind of load Types of Medicine electrostatic spinning guide tissue regeneration film answering in the reparative regeneration of periodontal and bone
With.
The advantage of the invention is that：
（1）The present invention loads cancer therapy drug using the HNTs with hollow structure as pharmaceutical carrier, can avoid simple medicine
The phenomenon of burst release that thing occurs with high polymer mixing Static Spinning so that the multiple guide tissue regeneration film of the Static Spinning of preparation has good
Therapeutic effect；
（2）Mechanical property of the inorganic HNTs of doping with composite cellulosic membrane can be improved in Static Spinning polymer nanofiber of the present invention
Can, and with good biocompatibility, degradability and promote bone uptake performance, be advantageous to medicament-carrying nano-fiber as function
Type tissue engineering bracket material is applied to environment inside complexity；
（3）Raw material HNTs in the present invention is a kind of cheap natural nano pipe of well-crystallized, green, and guiding group
It is simple to knit regeneration membrane preparation method technique, it is workable, it further can meet to produce and apply.
Embodiment
Technical scheme is further described below by way of specific embodiment.Following embodiment is to this
The further explanation of invention, and do not limit the scope of the invention.
Embodiment 1
1. carry medicine HNTs preparation
Take a certain amount of addition PMB to be dissolved in pure water and stir 20min, add a certain amount of HNTs solution, ultrasonic disperse afterwards
40min so that in the fully dispersed solution to PMB of HNTs so that HNTs and PMB concentration ratio is 1：2.PMB and HNTs is mixed
Solution is placed in vacuum drying chamber, is vacuumized under conditions of 0.05, and up to liquid surface, there is no bubble appearance.From vacuum drying
After case is taken out, it is repeated once and vacuumizes after stirring 5min, drive away the bubble of residual in HNTs internal cavities, make entering for more PMB
The inside entered.Centrifugation taking-up supernatant is stand-by under conditions of 3000rpm, and precipitation is cleaned with ultra-pure water.Medicinal powder end will be carried to exist
24h is dried under conditions of 50 DEG C.It is stand-by with using screen filtration after agate mortar mill-drying powder again.
2. the preparation of Static Spinning medicament-carrying composite nano-fiber membrane
Certain 0g PMB/HNTs, 0.23g GE and 0.46 g P (DLLA-co-CL) are added in 7.53g HFIP, are made into molten
Liquid, a homogeneous solution is obtained after stirring 6 ~ 12h at room temperature.Resulting solution is loaded in a 10 mL glass syringes,
The KV of spinning voltage 10 ~ 13, it is 12 ~ 15 cm that spinning, which receives distance, under the conditions of solution flow rate is 0.4 ~ 0.5 mL/h, is passed through
Electrospinning device obtains PMB/HNTs/ gelatin/P (DLLA-co-CL) composite nano-fiber membrane, by gained film after spinning
Be put into vacuum drying oven be dried overnight at room temperature it is standby.
Embodiment 2
1. carry medicine HNTs preparation
Take a certain amount of addition PMB to be dissolved in pure water and stir 20min, add a certain amount of HNTs solution, ultrasonic disperse afterwards
40min so that in the fully dispersed solution to PMB of HNTs so that HNTs and PMB concentration ratio is 1：2.PMB and HNTs is mixed
Solution is placed in vacuum drying chamber, is vacuumized under conditions of 0.05, and up to liquid surface, there is no bubble appearance.From vacuum drying
After case is taken out, it is repeated once and vacuumizes after stirring 5min, drive away the bubble of residual in HNTs internal cavities, make entering for more PMB
The inside entered.Centrifugation taking-up supernatant is stand-by under conditions of 3000rpm, and precipitation is cleaned with ultra-pure water.Medicinal powder end will be carried to exist
24h is dried under conditions of 50 DEG C.It is stand-by with using screen filtration after agate mortar mill-drying powder again.
2. the preparation of Static Spinning medicament-carrying composite nano-fiber membrane
Certain 0.145g PMB/HNTs, 0.23g GE and 0.46 g P (DLLA-co-CL) are added in 9.82g HFIP, matched somebody with somebody
Into solution, a homogeneous solution is obtained after stirring 6 ~ 12h at room temperature.Resulting solution is loaded into a 10 mL glass syringes
Interior, in the KV of spinning voltage 10 ~ 13, it is 12 ~ 15 cm that spinning, which receives distance, under the conditions of solution flow rate is 0.4 ~ 0.5 mL/h,
PMB/HNTs/ gelatin/P (DLLA-co-CL) composite nano-fiber membrane is obtained by electrospinning device, by institute after spinning
Film be put into vacuum drying oven be dried overnight at room temperature it is standby.
Embodiment 3
1. carry medicine HNTs preparation
Take a certain amount of addition PMB to be dissolved in pure water and stir 20min, add a certain amount of HNTs solution, ultrasonic disperse afterwards
40min so that in the fully dispersed solution to PMB of HNTs so that HNTs and PMB concentration ratio is 1：2.PMB and HNTs is mixed
Solution is placed in vacuum drying chamber, is vacuumized under conditions of 0.05, and up to liquid surface, there is no bubble appearance.From vacuum drying
After case is taken out, it is repeated once and vacuumizes after stirring 5min, drive away the bubble of residual in HNTs internal cavities, make entering for more PMB
The inside entered.Centrifugation taking-up supernatant is stand-by under conditions of 3000rpm, and precipitation is cleaned with ultra-pure water.Medicinal powder end will be carried to exist
24h is dried under conditions of 50 DEG C.It is stand-by with using screen filtration after agate mortar mill-drying powder again.
2. the preparation of Static Spinning medicament-carrying composite nano-fiber membrane
Certain 0.661g PMB/HNTs, 0.23g GE and 0.46 g P (DLLA-co-CL) are added in 14.07g HFIP, matched somebody with somebody
Into solution, a homogeneous solution is obtained after stirring 6 ~ 12h at room temperature.Resulting solution is loaded into a 10 mL glass syringes
Interior, in the KV of spinning voltage 10 ~ 13, it is 12 ~ 15 cm that spinning, which receives distance, under the conditions of solution flow rate is 0.4 ~ 0.5 mL/h,
PMB/HNTs/ gelatin/P (DLLA-co-CL) composite nano-fiber membrane is obtained by electrospinning device, by institute after spinning
Film be put into vacuum drying oven be dried overnight at room temperature it is standby.
Embodiment 4
1. carry medicine HNTs preparation
Take a certain amount of addition PMB to be dissolved in pure water and stir 20min, add a certain amount of HNTs solution, ultrasonic disperse afterwards
40min so that in the fully dispersed solution to PMB of HNTs so that HNTs and PMB concentration ratio is 1：1.PMB and HNTs is mixed
Solution is placed in vacuum drying chamber, is vacuumized under conditions of 0.05, and up to liquid surface, there is no bubble appearance.From vacuum drying
After case is taken out, it is repeated once and vacuumizes after stirring 5min, drive away the bubble of residual in HNTs internal cavities, make entering for more PMB
The inside entered.Centrifugation taking-up supernatant is stand-by under conditions of 3000rpm, and precipitation is cleaned with ultra-pure water.Medicinal powder end will be carried to exist
24h is dried under conditions of 50 DEG C.It is stand-by with using screen filtration after agate mortar mill-drying powder again.
2. the preparation of Static Spinning medicament-carrying composite nano-fiber membrane
Certain 0.145g PMB/HNTs, 0.23g GE and 0.46 g P (DLLA-co-CL) are added in 9.82g HFIP, matched somebody with somebody
Into solution, a homogeneous solution is obtained after stirring 6 ~ 12h at room temperature.Resulting solution is loaded into a 10 mL glass syringes
Interior, in the KV of spinning voltage 10 ~ 13, it is 12 ~ 15 cm that spinning, which receives distance, under the conditions of solution flow rate is 0.4 ~ 0.5 mL/h,
PMB/HNTs/ gelatin/P (DLLA-co-CL) composite nano-fiber membrane is obtained by electrospinning device, by institute after spinning
Film be put into vacuum drying oven be dried overnight at room temperature it is standby.
Claims (4)
1. a kind of preparation method for carrying Types of Medicine electrostatic spinning guide tissue regeneration film, it is characterised in that with natural nano pipe angstrom Lip river
Stone（HNTs）For raw material, contained medicine is polymyxins b（PMB）, comprise the following steps：
（1）Carry medicine HNTs preparation
Take a certain amount of PMB to be dissolved in pure water and stir 20min, add a certain amount of HNTs solution afterwards, ultrasonic disperse 40min,
So that in the fully dispersed solution to PMB of HNTs；PMB and HNTs mixed solutions are placed in vacuum drying chamber, under conditions of 0.05
Vacuumize, up to liquid surface, there is no bubble appearance；After vacuum drying chamber is taken out, it is repeated once and is taken out very after stirring 5min
Sky, drive away the bubble of residual in HNTs internal cavities, more PMB is entered inside；Centrifuge and take out under conditions of 3000rpm
Supernatant is stand-by, and precipitation is cleaned with ultra-pure water, will carry medicinal powder end and 24h is dried under conditions of 50 DEG C, ground with agate mortar dry
Use screen filtration after dry powder again, obtained PMB/HNTs is stand-by；
（2）The preparation of Static Spinning medicament-carrying composite nano-fiber membrane
PMB/the HNTs that will necessarily match, gelatin（GE）With it is poly-（Racemic lactic acid-co- caprolactones）（P(DLLA-co-CL)）Add
In HFIP, mass concentration 8-10% solution is made into, a homogeneous solution is obtained after stirring 6 ~ 12h at room temperature, by resulting solution
Load in a 10 mL glass syringes, in the KV of spinning voltage 10 ~ 13, it is 12 ~ 15 cm that spinning, which receives distance, solution flow rate
Under the conditions of 0.4 ~ 0.5 mL/h, PMB/HNTs/ gelatin/P (DLLA-co-CL) is compound is obtained by electrospinning device and received
Rice tunica fibrosa, gained film is put into vacuum drying oven after spinning be dried overnight at room temperature it is standby.
A kind of 2. load Types of Medicine electrostatic spinning guide tissue regeneration film according to claim 1 and preparation method thereof, it is characterised in that
Described HNTs is 100-300nm.
3. one kind carries Types of Medicine electrostatic spinning guide tissue regeneration film, it is characterised in that according to any methods described of claim 1 or 2
It is prepared, the inorganic doping material part of guide tissue regeneration film is HNTs, and organic material part is poly-（Racemic lactic acid-co-
Caprolactone）（P(DLLA-co-CL)）And gelatin（GE）.
4. Types of Medicine electrostatic spinning guide tissue regeneration film answering in the reparative regeneration of periodontal and bone is carried according to claim 3
With.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109675102A (en) * | 2018-11-22 | 2019-04-26 | 宋建康 | Load the guide tissue regeneration film and preparation method thereof of gelatin micro-nano ball |
| CN109825955A (en) * | 2019-02-03 | 2019-05-31 | 南通纺织丝绸产业技术研究院 | A kind of hypo-allergenic adult paper diaper of antibacterial and preparation method thereof |
| CN111249526A (en) * | 2020-01-20 | 2020-06-09 | 重庆大学 | Electrostatic spinning stent with effects of treating melanoma and repairing damaged tissues and preparation method thereof |
| CN112206342A (en) * | 2020-09-27 | 2021-01-12 | 广东泰宝医疗科技股份有限公司 | Alginate composite dressing and preparation method thereof |
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| CN111249526A (en) * | 2020-01-20 | 2020-06-09 | 重庆大学 | Electrostatic spinning stent with effects of treating melanoma and repairing damaged tissues and preparation method thereof |
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