CN107870215A - The gas chromatography combined with mass spectrometry analysis method of potassium cinnamate content in a kind of measure food - Google Patents
The gas chromatography combined with mass spectrometry analysis method of potassium cinnamate content in a kind of measure food Download PDFInfo
- Publication number
- CN107870215A CN107870215A CN201711218947.XA CN201711218947A CN107870215A CN 107870215 A CN107870215 A CN 107870215A CN 201711218947 A CN201711218947 A CN 201711218947A CN 107870215 A CN107870215 A CN 107870215A
- Authority
- CN
- China
- Prior art keywords
- sample
- potassium cinnamate
- extraction
- gas chromatography
- extracted
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N2030/062—Preparation extracting sample from raw material
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N2030/067—Preparation by reaction, e.g. derivatising the sample
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The present invention relates to a kind of gas chromatography combined with mass spectrometry analysis method for determining potassium cinnamate content in food, comprise the following steps：(1) weigh sample to be placed in container, add water constant volume, the pH of acid adding regulation sample solution is 1, then sample solution is extracted with dichloromethane, collects the dichloromethane layer after extraction, dries；(2) analyte derivative and extraction：Derivative reagent is added in dried sample into step (1), is vortexed and mixes, after being reacted in water-bath, room temperature is cooled to, adds n-hexane and extracted, supernatant is taken after standing as need testing solution；(3) determine：Potassium cinnamate content in the need testing solution obtained using gas chromatography combined with mass spectrometry technology to step (2) is measured.This method is operated by simplifying pre-treatment, and antijamming capability is good, and method precision is high, favorable reproducibility, qualitative accurate, can be widely applied to potassium cinnamate assay in food.
Description
Technical field
It is more particularly to a kind of to determine potassium cinnamate content in food the present invention relates to the analysis method of potassium cinnamate content
Gas chromatography-mass spectrography analysis method.
Background technology
Potassium cinnamate is a kind of conventional food additives.When it is combined with acidic materials, Chinese traditional medicine cinnamon will be generated
In active ingredient cinnamic acid.Research shows that cinnamic acid has a variety of medicinal effects, such as antitumor, antibacterial and suppression xanthine
Activity of oxidizing ferment etc..At present, cinnamic acid and its salt are all widely used on medicine, agricultural and food, as used in food
Make essence and flavoring agent, preservative etc..It is 0.05%~0.10% that it, which presses down mycostatic valid density, and general usual amounts are 0.10%
~0.20%, the valid density for suppressing bacterium is 0.01%~0.10%.Potassium cinnamate to cause food spoilage saccharomycete,
Mould, bacterial action are most strong, it is main suppress object also includes Pseudomycin category, enterobacteriaceae respectively belongs to, bacillus, breast
A variety of toxic mushrooms such as dry Pseudomonas and Escherichia coli and gram-negative, positive bacillus.Meanwhile potassium cinnamate is 2~7 in pH
Fungistatic effect it is fine, can be widely used in varieties of food items.
Potassium cinnamate belongs to non-toxic product, its LD50 much smaller than sorbic acid and (or) benzoic acid more conventional at present and its
Both salt, security are significantly higher.Potassium cinnamate is converted into phenylalanine after absorption of human body.Part phenylalanine can be with
It is automatically drained out in vitro by human body, a small amount of phenylalanine can be catalyzed generation to human body without any side effect through PAH
The tyrosine of effect.Therefore, potassium cinnamate will not be generated containing virose material, without potential poison as food preservative
Property, there is the characteristics of broad spectrum activity and safe and practical property
For the measure of potassium cinnamate content in food, there is not been reported at present, and in existing national standards, for food
The detection method and limit standard of middle potassium cinnamate also do not make specified in more detail.
The content of the invention
The Gas chromatographyMass spectrometry of potassium cinnamate content in food is determined it is an object of the invention to provide a kind of
Method, this method simplify pre-treatment operation, and antijamming capability is good, method precision by optimizing sample-pretreating method
Height, favorable reproducibility is qualitative accurate, can be widely applied to potassium cinnamate assay in food, to instruct to establish respective country mark
Standard provides reference.
It is an object of the invention to provide a kind of gas chromatography-mass spectrography analysis for determining potassium cinnamate content in food
Method, comprise the following steps：
(1) sample extraction：Weigh sample to be placed in container, add water constant volume, the pH of acid adding regulation sample solution is 1, then will
Sample solution is extracted with dichloromethane, collects the dichloromethane layer after extraction, is dried；
(2) analyte derivative and extraction：Derivative reagent is added in dried sample into step (1) to mix, in water-bath
Middle reaction, after being cooled to room temperature, add n-hexane and extracted, supernatant is taken after standing as need testing solution；
(3) determine：Cinnamic acid in the need testing solution obtained using Gas chromatographyMass spectrometry to step (2)
Potassium content is measured.
The present invention establishes a kind of simple, effectively, practical detection method, has filled up China in food inspection for Chinese cassia tree
The blank of sour potassium content assay method, reference is provided for Safety of Food Quality detection, and then ensure China's consumer's food security
With the sound development of China's food service industry.
The gas chromatography-mass spectrography analysis method of potassium cinnamate content, specifically includes following step in said determination food
Suddenly：
(1) sample extraction：Weigh 2g samples to be placed in container, add water to be settled to 25mL, acid adding adjusts the pH of sample solution
For 1, then sample solution extracted with dichloromethane, collect the dichloromethane layer after extraction, dry；
(2) analyte derivative and extraction：Derivative reagent is added in dried sample into step (1), is vortexed and mixes, in water
After reacting 10min in bath, after being cooled to room temperature, add 5mL n-hexanes and extracted, supernatant is taken after standing as confession
Test sample solution；
(3) determine：Potassium cinnamate in need testing solution is obtained to step (2) using Gas chromatographyMass spectrometry
Content is measured；GC conditions are：Chromatographic column：HP-5ms Ultra Inert 30m×0.25mm×0.25μm；Post
Temperature：100 DEG C of maintenance 2min, rise to 265 DEG C with 15 DEG C/min, maintain 3min；Injector temperature is 280 DEG C；The μ L of sample size 1；Point
Stream is than being 1：1；Carrier gas is helium, column flow：1.2mL/min；Mass Spectrometry Conditions：EI ion source temperatures：230℃；Level Four bar temperature
Degree：150℃；Solvent delay 3min；Scan mode：SIM, it is respectively 131,103,77,67 to select ion.
Preferably, the sample property described in step (1) is solid-state or liquid.
When sample is solid, sample extraction concretely comprises the following steps in step (1)：Weigh 2g solid samples and be placed in 50mL
In centrifuge tube, 5mL deionized waters are added, are mixed, ultrasonic extraction 5min, filtering, filtrate are settled to 25mL, then to filtrate
Middle addition 2mol/L sulfuric acid, regulation pH value of solution are 1, then extracted 3 times with dichloromethane, collect dichloromethane layer, drying.
When sample is liquid, sample extraction concretely comprises the following steps in step (1)：Weigh 2g fluid samples and be placed in 50mL
In centrifuge tube, add deionized water and be settled to 25mL, 2mol/L sulfuric acid is then added into sample solution, adjust pH value of solution
For 1, then extracted 3 times with dichloromethane, collect dichloromethane layer, drying.
Preferably, derivative reagent described in step (2) is the 1mL concentrated sulfuric acid and 2.5mL absolute ethyl alcohol.Sample pre-treatments
During pre-treatment carried out to sample using the concentrated sulfuric acid-ethanol derivatization method, determinand is carried out ethyl esterified, improves the symmetrical of peak
Property, chromatographic peak profile is effectively improved, and the sensitivity of detection method is improved to a certain extent, chromatographic peak after cinnamic acid derives
7.8 times of a height of not derivative cinnamic acid peak height.
Further preferred, analyte derivative described in step (2) concretely comprises the following steps with extraction：Dried into step (1)
2.5mL absolute ethyl alcohol and the 1mL concentrated sulfuric acid are sequentially added in sample afterwards, is mixed, after reacting 10min in water-bath,
After being cooled to room temperature, add 5mL n-hexanes and extracted, supernatant is taken after standing as need testing solution.
The method of the present invention overcomes the deficiency of prior art sample treatment, and compared with prior art, the present invention has
Following excellent results：
1st, a kind of simple, effectively, practical detection method is established, has filled up China in food inspection for potassium cinnamate
The blank of content assaying method, reference is provided for Safety of Food Quality detection, and then ensure China's consumer's food security and I
The sound development of state's food service industry.
2nd, handled before the inventive method is carried out in sample pretreatment process using the concentrated sulfuric acid-ethanol derivatization method to sample,
Determinand is carried out ethyl esterified, improve the symmetry at peak, effectively improve chromatographic peak profile, and improve inspection to a certain extent
The sensitivity of survey method, 7.8 times of a height of not derivative cinnamic acid peak height of chromatographic peak after cinnamic acid derives.
3rd, the inventive method can determine the potassium cinnamate in food, and it has a wide range of application, and can be widely applied in food
Detection.
4th, the detection limit of the inventive method is low：Potassium cinnamate standard solution is constantly diluted into sample introduction, until the material
Concentration when peak height is 3 times of noise is detection limit, and now the concentration of potassium cinnamate solution is 0.04 μ gmL-1, with sample
Measure and counted for 2.0g, in food the detection of potassium cinnamate be limited to 0.1 μ gg-1。
5th, the inventive method is reproducible, and recovery of standard addition meets the requirement of analysis measure.
Brief description of the drawings
Fig. 1 is the operational flowchart (solid sample pre-treatment flow chart) that inventive samples are solid；
Fig. 2 is the operational flowchart (fluid sample pre-treatment flow chart) that inventive samples are liquid；
Fig. 3 is that gas chromatography-mass spectrum compares figure (gas phase color before and after potassium cinnamate derivatization before and after potassium cinnamate derivatization
Spectrum-mass spectrogram)；
Fig. 4 is gas chromatography-mass spectrum figure (cinnamic acid standard items, the sample of potassium cinnamate standard items, Chinese cassia tree sugar-like product and blank
Product and blank total ion current figure), wherein 1 represents ethyl cinnamate.
Specific implementation method
The present invention is further described by specific examples below, but does not limit the present invention.
Laboratory apparatus, reagent and condition of work
1) instrument
Agilent 7890B gas chromatographs are furnished with 5977B mass detectors (Agilent company of the U.S.)；WBK-4B types electricity
Hot thermostat water bath (Huankai Microbes Tech Co., Ltd., Guangdong)；Ultrasonic washing instrument (the limited public affairs of Shanghai Luxi analytical instrument
Department)；Centrifuge TDZ5-WS (Hunan C1-esteraseremmer-N Xiang Yi Science and Technology Ltd.s)；Micro-whirlpool mixed instrument XW-80A (Shanghai Luxis point
Analyse Instrument Ltd.)；Sartorius electronic balances (BSA224S) (Guangzhou Shen Hua experimental instrument and equipments Co., Ltd).
2) reagent
Potassium cinnamate (U.S.'s Aladdin, 99.0%)；The concentrated sulfuric acid, n-hexane, dichloromethane, absolute ethyl alcohol are analysis
It is pure, buy in Guangzhou chemical reagent work.
3) chromatographic condition
Chromatographic column：HP-5ms Ultra Inert 30m×0.25mm×0.25μm；Column temperature：100 DEG C of maintenance 2min, with 15
DEG C/min rises to 265 DEG C, maintain 3min；Injector temperature is 280 DEG C；The μ L of sample size 1；Split ratio is 1：1；Carrier gas is helium,
Column flow：1.2mL/min.Mass Spectrometry Conditions：EI ion guns, temperature：230℃；Level Four bar temperature：150℃；Solvent delay 3min；
Scan mode：SIM, ion is selected to be divided into 131,103,77,67.
Embodiment 1
As shown in figures 1-4：
1st, when sample is solid, as shown in figure 1, the pre-treatment of sample, specifically comprises the following steps：
(1) sample extraction：Weigh 2g samples to be placed in centrifuge tube, add 5mL deionized waters and mix, ultrasonic extraction 5min,
Filter to get filtrate, filtrate is settled to 25mL, 1mL 2mol/L sulfuric acid is added into filtrate, the pH for adjusting filtrate is 1, will
Filtrate is extracted 3 times with dichloromethane, collects the dichloromethane layer after extraction, drying；
(2) analyte derivative and extraction：The 1mL concentrated sulfuric acids, 2.5mL to be sequentially added in the sample of drying anhydrous into step (1)
Ethanol, it is vortexed and mixes, after reacting 10min in water-bath, after being cooled to room temperature, adds 5mL n-hexanes and extracted, it is quiet
Postpone and take supernatant as need testing solution.
When sample is liquid, as shown in Fig. 2 the pre-treatment of sample, specifically comprises the following steps：
(1) sample extraction：Weigh 2g fluid samples to be placed in 50mL centrifuge tubes, add deionized water and be settled to 25mL, so
2mol/L sulfuric acid is added in backward sample solution, regulation pH value of solution is 1, then extracted 3 times with dichloromethane, collects dichloromethane
Alkane layer, drying；
(2) analyte derivative and extraction：The 1mL concentrated sulfuric acids, 2.5mL to be sequentially added in the sample of drying anhydrous into step (1)
Ethanol, it is vortexed and mixes, after reacting 10min in water-bath, after being cooled to room temperature, adds 5mL n-hexanes and extracted, it is quiet
Postpone and take supernatant as need testing solution.
2nd, the preparation of standard working solution and the determination of standard working curve
(1) preparation of potassium cinnamate standard liquid
Precision weighs dried potassium cinnamate 25.03mg, puts in 25mL volumetric flasks, adds distilled water to dissolve and is diluted to quarter
25mL is spent, makees that storing solution is standby, needs to dilute mother liquor step by step according to experiment.
(2) determination of standard working curve
The accurate potassium cinnamate standard solution for drawing different volumes respectively, is handled by aforesaid liquid sample-pretreating method
A series of standard working solution of potassium cinnamates is obtained, and is measured using gas chromatograph-mass spectrometer (GC-MS), result is carried out
Analysis, with peak area A to concentration C drawing curve, potassium cinnamate concentration is in 0.1001~100.1 μ gmL-1Scope, meat
The concentration of cinnamic acid potassium-peak area regression equation is A=58321C+24474, R2=0.9999, the results showed that peak area and Chinese cassia tree
The concentration of sour potassium is in good linear relation, as shown in table 1.
The relation of the potassium cinnamate standard liquid peak area of table 1 and concentration
(3) peak area of need testing solution is determined, the meat in sample is calculated in the working curve obtained by step (2)
Cinnamic acid potassium content, inventor determine the content of potassium cinnamate using numerous food (solid-state or liquid) as sample, the results are shown in Table 2.
Potassium cinnamate assay result in the different food products of table 2
| Sample name | Content (μ g/g) | Sample name | Content (μ g/g) | Sample name | Content (μ g/g) |
| Soy sauce | N.D. | Vitamin fruit-flavored beverage | N.D. | Dried orange peel cake | N.D. |
| Chinese cassia tree sugar | 3.828 | Dried sweet potato | N.D. | Fragrant citrus is crisp | N.D. |
| Preserved plum vinegar | N.D. | Butyrum heart sugar | N.D. | Mature vinegar | N.D. |
| Raw meat bag | N.D. | Swiss roll | N.D. | Betel nut | 0.288 |
| Cake is rolled up | N.D. | Fruit orange | N.D. | Dried pork slice | N.D. |
| Wahaha | N.D. | Finda orange taste carbonated drink | N.D. | Half of plum | N.D. |
| Slip plum | N.D. | Watson sarsaparilla carbonated drink | N.D. | Dried orange peel preserved plum | N.D. |
| Preserved pears | N.D. | Coca-Cola | N.D. | Pepsi Cola | N.D. |
Wherein：" N.D. " represents not detect.
The present invention is not limited to this several sample pointed out in the present embodiment, art technology to the protection domain of determination sample
The sample that personnel are contemplated that is within protection scope of the present invention.
Embodiment 2
Repeatability and average recovery experiment：
Take Chinese cassia tree sugar to supply 6 parts of sample, carry out sample pre-treatments and gas chromatography-mass spectrometry analysis respectively, and according to mark-on amount
The rate of recovery is calculated with measured value, the average content for determining potassium cinnamate in Chinese cassia tree sugar is that (relative standard is inclined by 3.839 μ g/g, RSD
Difference) it is 1.59%, the results showed that and this method repeatability is good, and the rate of recovery the results are shown in Table 3.
The recovery testu result (n=6) of table 3
As shown in Table 3, in Chinese cassia tree sugar the mark-on average recovery rate of potassium cinnamate up to 103.5%, RSD 6.24%, the party
Method mark-on average recovery rate can meet the analysis requirement for determining potassium cinnamate content in Chinese cassia tree sugar, be adapted to potassium cinnamate in food
Content analysis.
It can be drawn by Fig. 3, pre-treatment is carried out to sample using the concentrated sulfuric acid-ethanol derivatization method in sample pretreatment process, will
Determinand progress is ethyl esterified, improves the symmetry at peak, effectively improves chromatographic peak profile, and improve detection to a certain extent
The sensitivity of method, 7.8 times of a height of not derivative cinnamic acid peak height of chromatographic peak after cinnamic acid derives.As shown in Figure 4, cinnamic acid
Ethyl ester separating degree is good, and blank is noiseless, the assay of cinnamic acid suitable for food.
Above-listed detailed description is illustrating for possible embodiments of the present invention, and the embodiment simultaneously is not used to limit this hair
Bright the scope of the claims, all equivalence enforcements or change without departing from carried out by the present invention, it is intended to be limited solely by the scope of patent protection of this case
In.
Claims (7)
1. it is a kind of determine food in potassium cinnamate content gas chromatography-mass spectrography analysis method, it is characterised in that including with
Lower step：
(1) sample extraction：Weighing sample to be placed in container, add water constant volume, the pH of acid adding regulation sample solution is 1, then by sample
Solution is extracted with dichloromethane, collects the dichloromethane layer after extraction, is dried；
(2) analyte derivative and extraction：Derivative reagent is added in dried sample into step (1) to mix, it is anti-in water-bath
Ying Hou, room temperature is cooled to, adds n-hexane and extracted, supernatant is taken after standing as need testing solution；
(3) determine：Potassium cinnamate in the need testing solution obtained using Gas chromatographyMass spectrometry to step (2) is contained
Amount is measured.
2. the gas chromatography-mass spectrography analysis method of potassium cinnamate content in measure food according to claim 1, its
It is characterised by, comprises the following steps：
(1) sample extraction：Weighing 2g samples to be placed in container, add water to be settled to 25mL, the pH of acid adding regulation sample solution is 1,
Sample solution is extracted with dichloromethane again, collects the dichloromethane layer after extraction, is dried；
(2) analyte derivative and extraction：Derivative reagent is added in dried sample into step (1) to mix, it is anti-in water-bath
After answering 10min, after being cooled to room temperature, add 5mL n-hexanes and extracted, supernatant is taken after standing as need testing solution；
(3) determine：Potassium cinnamate in the need testing solution obtained using Gas chromatographyMass spectrometry to step (2) is contained
Amount is measured；GC conditions are：Chromatographic column：HP-5ms Ultra Inert 30m×0.25mm×0.25μm；Column temperature：
100 DEG C of maintenance 2min, rise to 265 DEG C with 15 DEG C/min, maintain 3min；Injector temperature is 280 DEG C；The μ L of sample size 1；Split ratio
For 1：1；Carrier gas is helium, column flow：1.2mL/min；Mass Spectrometry Conditions：EI ion source temperatures：230℃；Level Four bar temperature：150
℃；Solvent delay 3min；Scan mode：SIM, it is respectively 131,103,77,67 to select ion.
3. the gas chromatography-mass spectrography analysis side of potassium cinnamate content in measure food according to claim 1 or 2
Method, it is characterised in that the sample property described in step (1) is solid-state or liquid.
4. the gas chromatography-mass spectrography analysis method of potassium cinnamate content in measure food according to claim 3, its
It is characterised by, sample extraction concretely comprises the following steps described in step (1)：Weigh 2g solid samples to be placed in 50mL centrifuge tubes, add
Enter 5mL deionized waters, mix, ultrasonic extraction 5min, filtering, filtrate is settled to 25mL, 2mol/L is then added into filtrate
Sulfuric acid, regulation pH value of solution be 1, then with dichloromethane extract 3 times, collect dichloromethane layer, drying.
5. the gas chromatography-mass spectrography analysis method of potassium cinnamate content in measure food according to claim 3, its
It is characterised by, sample extraction concretely comprises the following steps described in step (1)：Weigh 2g fluid samples to be placed in 50mL centrifuge tubes, add
Enter deionized water and be settled to 25mL, 2mol/L sulfuric acid is then added into sample solution, regulation pH value of solution is 1, then uses dichloro
Methane extracts 3 times, collects dichloromethane layer, drying.
6. the gas chromatography-mass spectrography analysis side of potassium cinnamate content in measure food according to claim 1 or 2
Method, derivative reagent described in step (2) are 2.5mL absolute ethyl alcohol and the 1mL concentrated sulfuric acid.
7. the gas chromatography-mass spectrography analysis method of potassium cinnamate content, step in measure food according to claim 6
Suddenly analyte derivative described in (2) concretely comprises the following steps with what is extracted：Into step (1) 2.5mL is sequentially added in dried sample
Absolute ethyl alcohol and 1mL the concentrated sulfuric acid, mix, in water-bath react 10min after, after being cooled to room temperature, add 5mL just oneself
Alkane is extracted, and supernatant is taken after standing as need testing solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711218947.XA CN107870215A (en) | 2017-11-28 | 2017-11-28 | The gas chromatography combined with mass spectrometry analysis method of potassium cinnamate content in a kind of measure food |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711218947.XA CN107870215A (en) | 2017-11-28 | 2017-11-28 | The gas chromatography combined with mass spectrometry analysis method of potassium cinnamate content in a kind of measure food |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107870215A true CN107870215A (en) | 2018-04-03 |
Family
ID=61754788
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711218947.XA Pending CN107870215A (en) | 2017-11-28 | 2017-11-28 | The gas chromatography combined with mass spectrometry analysis method of potassium cinnamate content in a kind of measure food |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107870215A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101042376A (en) * | 2006-03-20 | 2007-09-26 | 中国人民解放军军事医学科学院放射与辐射医学研究所 | Method and reagent kit for rapid measuring protein N terminal sequence |
| CN103063764A (en) * | 2012-12-21 | 2013-04-24 | 林维宣 | Detection method for residual quantity of multiple preservatives in cosmetics |
| CN103529155A (en) * | 2013-09-27 | 2014-01-22 | 中国检验检疫科学研究院 | Method for measuring content of wood preservatives of wooden products for children |
| CN104280502A (en) * | 2014-10-28 | 2015-01-14 | 云南中烟工业有限责任公司 | Method for separating and detecting phthalate esters compounds in hot melt glue |
| CN106596803A (en) * | 2016-11-16 | 2017-04-26 | 方贵雄 | Method for detecting sorbic acid in food |
| CN107192769A (en) * | 2017-04-11 | 2017-09-22 | 河南省粮油饲料产品质量监督检验中心 | Propionic acid, sorbic acid, benzoic acid, the method for dehydroactic acid content in a kind of Rapid Simultaneous Determination food |
| CN107389817A (en) * | 2017-07-13 | 2017-11-24 | 浙江省疾病预防控制中心 | Preservative associated detecting method in food |
-
2017
- 2017-11-28 CN CN201711218947.XA patent/CN107870215A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101042376A (en) * | 2006-03-20 | 2007-09-26 | 中国人民解放军军事医学科学院放射与辐射医学研究所 | Method and reagent kit for rapid measuring protein N terminal sequence |
| CN103063764A (en) * | 2012-12-21 | 2013-04-24 | 林维宣 | Detection method for residual quantity of multiple preservatives in cosmetics |
| CN103529155A (en) * | 2013-09-27 | 2014-01-22 | 中国检验检疫科学研究院 | Method for measuring content of wood preservatives of wooden products for children |
| CN104280502A (en) * | 2014-10-28 | 2015-01-14 | 云南中烟工业有限责任公司 | Method for separating and detecting phthalate esters compounds in hot melt glue |
| CN106596803A (en) * | 2016-11-16 | 2017-04-26 | 方贵雄 | Method for detecting sorbic acid in food |
| CN107192769A (en) * | 2017-04-11 | 2017-09-22 | 河南省粮油饲料产品质量监督检验中心 | Propionic acid, sorbic acid, benzoic acid, the method for dehydroactic acid content in a kind of Rapid Simultaneous Determination food |
| CN107389817A (en) * | 2017-07-13 | 2017-11-24 | 浙江省疾病预防控制中心 | Preservative associated detecting method in food |
Non-Patent Citations (6)
| Title |
|---|
| CHRISTINEH N. SARKISSIAN 等: "Quantitation of phenylalanine and its trans-cinnamic, benzoic and hippuric acid metabolites in biological fluids in a single GC-MS analysis", 《J. MASS SPECTROM.》 * |
| I. PASTOROVA 等: "Analytical Study of Free and Ester Bound Benzoic and Cinnamic Acids of Gum Benzoin Resins by GC–MS and HPLC–frit FAB–MS", 《PHYTOCHEMICAL ANALYSIS》 * |
| MOHAMMAD SARAJI 等: "Single-drop microextraction followed by in-syringe derivatization and gas chromatography-mass spectrometric detection for determination of organic acids in fruits and fruit juices", 《J. SEP. SCI.》 * |
| 张存玲 等: "衍生气相色谱法在食品卫生检验中的应用", 《中国食品卫生杂志》 * |
| 李春发 等: "肉桂酸钾在软饮料中的应用", 《宁夏农林科技》 * |
| 杨昌金 等: "衍生化-气相色谱法测定糖浆剂中苯甲酸与苯甲酸钠含量", 《色谱》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Lan et al. | Evaluation of antioxidant capacity and flavor profile change of pomegranate wine during fermentation and aging process | |
| Kataoka et al. | Determination of patulin in fruit juice and dried fruit samples by in-tube solid-phase microextraction coupled with liquid chromatography–mass spectrometry | |
| Mollakhalili-Meybodi et al. | Acrylamide in bread: a review on formation, health risk assessment, and determination by analytical techniques | |
| CN107907600B (en) | Method for simultaneously measuring aflatoxin and flavoring agent in vegetable oil based on liquid-liquid extraction-liquid chromatography-tandem mass spectrometry | |
| CN106338552A (en) | Detection method for 3-MCPD ester in oil product | |
| CN111308004A (en) | Identification method for differences of volatile flavor components of marinated food | |
| CN114137134A (en) | Method for separating selenium form by high performance liquid chromatography-inductively coupled plasma mass spectrometry | |
| Roach et al. | Capillary gas chromatography/mass spectrometry with chemical ionization and negative ion detection for confirmation of identity of patulin in apple juice | |
| CN103869015A (en) | Method for quantitatively determining 14 types of trans-2-aldehydes and diolefine aldehydes compounds in white spirit | |
| CN107870215A (en) | The gas chromatography combined with mass spectrometry analysis method of potassium cinnamate content in a kind of measure food | |
| Surh et al. | Simultaneous determination of 4-hydroxy-2-alkenals, lipid peroxidation toxic products | |
| CN101718764A (en) | Detection method of residual polychlorinated biphenyl in cosmetics with gas chromatography-mass spectrum method | |
| Mandrou et al. | Thin layer chromatography reflectometric determination of benzoic and sorbic acids in fruit beverages | |
| CN105954434A (en) | Method for detecting phenols spice | |
| CN110927269A (en) | Method for quantitatively detecting DL-2-hydroxy-4-methyl ethyl valerate in wine | |
| CN105699580A (en) | Method for measuring organic acid content based on precolumn derivatization LC-MS | |
| Ramalho et al. | Content of caffeine in the edible mushroom Pleurotus ostreatus grown in coffee residues | |
| CN111060630B (en) | Method for measuring moisture content in water-based paint based on GC-MS | |
| CN107102078A (en) | A kind of method of aflatoxin B1 in measure Gardenia Yellow | |
| CN113588820A (en) | Detection method for simultaneously determining three toxic aldehydes in oil-based food | |
| CN106770780A (en) | The detection method of active material in precious Rogor raw material or the product containing precious Rogor | |
| CN112014497A (en) | Method for simultaneously detecting three monoglyceride emulsifiers in dairy product based on gas chromatography-mass spectrometry | |
| CN104280501B (en) | In a kind of sugar-curing cherry tomato, cycocel removes and detection method | |
| CN108693287B (en) | A kind of method of antioxidant content in detection white wine | |
| CN103063770A (en) | Method for detecting antioxidants in vegetable oil or animal fat |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180403 |
|
| RJ01 | Rejection of invention patent application after publication |