CN107869046A - A kind of fibroin method of modifying of polyester fabric - Google Patents
A kind of fibroin method of modifying of polyester fabric Download PDFInfo
- Publication number
- CN107869046A CN107869046A CN201711224540.8A CN201711224540A CN107869046A CN 107869046 A CN107869046 A CN 107869046A CN 201711224540 A CN201711224540 A CN 201711224540A CN 107869046 A CN107869046 A CN 107869046A
- Authority
- CN
- China
- Prior art keywords
- fabric
- fibroin
- fibroin albumen
- destarch
- silk
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108010022355 Fibroins Proteins 0.000 title claims abstract description 177
- 239000004744 fabric Substances 0.000 title claims abstract description 107
- 238000000034 method Methods 0.000 title claims abstract description 57
- 229920000728 polyester Polymers 0.000 title claims abstract description 56
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 53
- 230000008569 process Effects 0.000 claims abstract description 23
- 238000011282 treatment Methods 0.000 claims abstract description 23
- 239000003513 alkali Substances 0.000 claims abstract description 22
- 238000006243 chemical reaction Methods 0.000 claims abstract description 13
- 239000003431 cross linking reagent Substances 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 78
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 50
- 241000255789 Bombyx mori Species 0.000 claims description 33
- 239000000243 solution Substances 0.000 claims description 26
- 239000007864 aqueous solution Substances 0.000 claims description 25
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 25
- 229920001503 Glucan Polymers 0.000 claims description 24
- 238000010828 elution Methods 0.000 claims description 15
- 238000012545 processing Methods 0.000 claims description 15
- 229920005654 Sephadex Polymers 0.000 claims description 14
- 239000012507 Sephadex™ Substances 0.000 claims description 14
- 238000012216 screening Methods 0.000 claims description 14
- 238000001962 electrophoresis Methods 0.000 claims description 11
- 239000006166 lysate Substances 0.000 claims description 11
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 claims description 11
- 150000001718 carbodiimides Chemical class 0.000 claims description 10
- AMXOYNBUYSYVKV-UHFFFAOYSA-M lithium bromide Chemical compound [Li+].[Br-] AMXOYNBUYSYVKV-UHFFFAOYSA-M 0.000 claims description 10
- 230000007935 neutral effect Effects 0.000 claims description 8
- 230000001954 sterilising effect Effects 0.000 claims description 8
- 239000012266 salt solution Substances 0.000 claims description 7
- 238000001819 mass spectrum Methods 0.000 claims description 6
- 238000010612 desalination reaction Methods 0.000 claims description 4
- 238000011067 equilibration Methods 0.000 claims description 4
- 238000004090 dissolution Methods 0.000 claims description 3
- JOAISNMPBNLOCX-UHFFFAOYSA-N [Ca].CCO Chemical compound [Ca].CCO JOAISNMPBNLOCX-UHFFFAOYSA-N 0.000 claims 1
- 238000005660 chlorination reaction Methods 0.000 claims 1
- 150000003384 small molecules Chemical class 0.000 claims 1
- 239000000835 fiber Substances 0.000 abstract description 16
- 239000000463 material Substances 0.000 description 31
- 239000008367 deionised water Substances 0.000 description 29
- 229910021641 deionized water Inorganic materials 0.000 description 29
- 239000011259 mixed solution Substances 0.000 description 18
- 230000002572 peristaltic effect Effects 0.000 description 17
- 238000001035 drying Methods 0.000 description 14
- 239000011521 glass Substances 0.000 description 13
- 239000003480 eluent Substances 0.000 description 11
- 238000005406 washing Methods 0.000 description 11
- GDESEHSRICGNDP-UHFFFAOYSA-L [Cl-].[Cl-].[Ca+2].CCO Chemical compound [Cl-].[Cl-].[Ca+2].CCO GDESEHSRICGNDP-UHFFFAOYSA-L 0.000 description 8
- 238000009835 boiling Methods 0.000 description 8
- 238000009826 distribution Methods 0.000 description 8
- 230000037396 body weight Effects 0.000 description 7
- 235000019786 weight gain Nutrition 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 6
- 238000001816 cooling Methods 0.000 description 6
- 238000005374 membrane filtration Methods 0.000 description 6
- 230000004048 modification Effects 0.000 description 6
- 238000012986 modification Methods 0.000 description 6
- 239000012460 protein solution Substances 0.000 description 6
- 239000004753 textile Substances 0.000 description 6
- 239000011800 void material Substances 0.000 description 6
- 238000009941 weaving Methods 0.000 description 6
- 101710198774 Envelope protein US9 Proteins 0.000 description 5
- 239000011859 microparticle Substances 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- NQTADLQHYWFPDB-UHFFFAOYSA-N N-Hydroxysuccinimide Chemical compound ON1C(=O)CCC1=O NQTADLQHYWFPDB-UHFFFAOYSA-N 0.000 description 4
- 229920004933 Terylene® Polymers 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 4
- 229940059936 lithium bromide Drugs 0.000 description 4
- 239000005020 polyethylene terephthalate Substances 0.000 description 4
- 230000018044 dehydration Effects 0.000 description 3
- 238000006297 dehydration reaction Methods 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 239000008399 tap water Substances 0.000 description 3
- 235000020679 tap water Nutrition 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000035484 reaction time Effects 0.000 description 2
- 229920002994 synthetic fiber Polymers 0.000 description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 230000002844 continuous effect Effects 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000003507 refrigerant Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000012209 synthetic fiber Substances 0.000 description 1
Classifications
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M11/00—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with inorganic substances or complexes thereof; Such treatment combined with mechanical treatment, e.g. mercerising
- D06M11/32—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with inorganic substances or complexes thereof; Such treatment combined with mechanical treatment, e.g. mercerising with oxygen, ozone, ozonides, oxides, hydroxides or percompounds; Salts derived from anions with an amphoteric element-oxygen bond
- D06M11/36—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with inorganic substances or complexes thereof; Such treatment combined with mechanical treatment, e.g. mercerising with oxygen, ozone, ozonides, oxides, hydroxides or percompounds; Salts derived from anions with an amphoteric element-oxygen bond with oxides, hydroxides or mixed oxides; with salts derived from anions with an amphoteric element-oxygen bond
- D06M11/38—Oxides or hydroxides of elements of Groups 1 or 11 of the Periodic Table
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06L—DRY-CLEANING, WASHING OR BLEACHING FIBRES, FILAMENTS, THREADS, YARNS, FABRICS, FEATHERS OR MADE-UP FIBROUS GOODS; BLEACHING LEATHER OR FURS
- D06L1/00—Dry-cleaning or washing fibres, filaments, threads, yarns, fabrics, feathers or made-up fibrous goods
- D06L1/12—Dry-cleaning or washing fibres, filaments, threads, yarns, fabrics, feathers or made-up fibrous goods using aqueous solvents
- D06L1/14—De-sizing
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M13/00—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with non-macromolecular organic compounds; Such treatment combined with mechanical treatment
- D06M13/322—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with non-macromolecular organic compounds; Such treatment combined with mechanical treatment with compounds containing nitrogen
- D06M13/402—Amides imides, sulfamic acids
- D06M13/432—Urea, thiourea or derivatives thereof, e.g. biurets; Urea-inclusion compounds; Dicyanamides; Carbodiimides; Guanidines, e.g. dicyandiamides
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M15/00—Treating fibres, threads, yarns, fabrics, or fibrous goods made from such materials, with macromolecular compounds; Such treatment combined with mechanical treatment
- D06M15/01—Treating fibres, threads, yarns, fabrics, or fibrous goods made from such materials, with macromolecular compounds; Such treatment combined with mechanical treatment with natural macromolecular compounds or derivatives thereof
- D06M15/15—Proteins or derivatives thereof
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M2101/00—Chemical constitution of the fibres, threads, yarns, fabrics or fibrous goods made from such materials, to be treated
- D06M2101/16—Synthetic fibres, other than mineral fibres
- D06M2101/30—Synthetic polymers consisting of macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
- D06M2101/32—Polyesters
Landscapes
- Engineering & Computer Science (AREA)
- Textile Engineering (AREA)
- Cosmetics (AREA)
- Treatments For Attaching Organic Compounds To Fibrous Goods (AREA)
Abstract
The invention provides a kind of fibroin method of modifying of polyester fabric, including：A) polyester fabric destarch is handled, obtains the fabric of destarch；B the fabric of destarch) is used into naoh treatment, washs, obtains the fabric after alkali process；C) fabric after alkali process is impregnated in cross-linking agent solution, the fibroin albumen hybrid reaction with 1000~5000Da molecular weight.The fabric that method of modifying provided by the invention handles destarch uses naoh treatment, and the setting of above-mentioned alkali process to discharge reaction site on polyester fiber strand；Simultaneously, the present invention is modified the fabric after alkali process using the fibroin albumen of the 1000~5000Da molecular weight limited, the fibroin albumen of above-mentioned specific small-molecular-weight is caused firmly to introduce the fiber surface of polyester fabric using covalently bound principle, nice and cool property, flexibility, the antistatic behaviour of polyester fabric are improved, while mechanical property is good, silk-fibroin stability is good.
Description
Technical field
The present invention relates to textile technology field, more particularly, to a kind of fibroin method of modifying of polyester fabric.
Background technology
Terylene is textile fabric yield highest fiber, is the most important raw material of textile product, and accounting textile fabric is processed
More than the 70% of amount, polyester fiber is chemical synthetic fiber, because high with fracture strength, light resistance is good, and it is excellent that product is well-pressed etc.
Point and be widely used in and take and household textiles, mainly have overcoat, curtain, sofa fabric, in-car ornaments etc., but terylene category
In super-hydrophobicity fiber, hygroscopicity extreme difference, rigidity, limit its application in home textile and some garment industries, as summer cool clothes,
Underwear and bedding etc..
Prior art reported the research of fibroin albumen coating polyester, had after progress polyester fiber or fabric coating crisp
Property, it is easily rupturable and come off, and the surface texture even gas permeability of original fabric can be influenceed.Also the fibroin albumen of reported in literature applies
It is low to cover rate of body weight gain, it is water-fastness undesirable, or regain is still very low.So there is no market application.
The content of the invention
In view of this, the technical problem to be solved in the present invention is the fibroin modification side for providing a kind of polyester fabric
Method, polyester fabric mechanical property that method of modifying provided by the invention is modified is good, stability is good.
The invention provides a kind of fibroin method of modifying of polyester fabric, including：
A) polyester fabric destarch is handled, obtains the fabric of destarch；
B the fabric of destarch) is used into naoh treatment, washs, obtains the fabric after alkali process；
C) fabric after alkali process is impregnated in cross-linking agent solution, mixed with the fibroin albumen of 1000~5000Da molecular weight
Close reaction.
Preferably, the crosslinking agent includes carbodiimides.
Preferably, the mass percent that the carbodiimides accounts for fibroin albumen is 20%~40%.
Preferably, step A) destarch is specially to be handled using sodium carbonate destarch；The concentration of the sodium carbonate liquor is
6g/L；The destarch treatment temperature is 90~100 DEG C；The destarch processing time is 1h.
Preferably, step B) mass concentration of the sodium hydroxide is 5%~30%；The treatment temperature of the sodium hydroxide
For 30~40 DEG C；The processing time of the sodium hydroxide is 1~3h.
Preferably, step C) reaction temperature is 25~35 DEG C；The reaction time is 1~3h.
Preferably, the fibroin albumen of 1000~5000Da molecular weight is prepared as follows：
A) silkworm silk is subjected to degumming using sodium carbonate and then adds neutral salt solution dissolving, it is molten to prepare fibroin albumen
Solve liquid；
Glucan Sephadex G50 and G25 column equilibrations is stand-by；
B) fibroin albumen lysate is added in glucan Sephadex G50 posts, with sterilizing water elution fibroin albumen, divided
Pipe collects the silk fibroin water solution of desalination；
C) using the fibroin albumen of below 10kD in SDS-PAGE electrophoresis or mass spectrum screening step b) collecting pipes, add again
In glucan Sephadex G25 posts, with sterilizing water elution fibroin albumen, it is in charge of collection, screening obtains the small of 1000~5000Da
Molecular weight fibroin albumen.
Preferably, the step a) neutral salt solutions are selected from lithium bromide or calcium chloride-ethanol；The dissolution time is 5~7
Hour；The fibroin albumen lysate concentration is 10~50mg/mL.
Preferably, also include after below 10kD fibroin albumen in step c) the screening step b) collecting pipes it is lyophilized and
After be configured to 30~80mg/mL aqueous solution.
Preferably, the step b) elution flow rates are 200~400mL/h；Step c) the elution flow rates are 30~70mL/
h。
Compared with prior art, the invention provides a kind of fibroin method of modifying of polyester fabric, including：A) will wash
The destarch of synthetic fibre fabric is handled, and obtains the fabric of destarch；B the fabric of destarch) is used into naoh treatment, obtains the face after alkali process
Material；C) fabric after alkali process is impregnated in cross-linking agent solution, mixed instead with the fibroin albumen of 1000~5000Da molecular weight
Should.The fabric that method of modifying provided by the invention handles destarch uses naoh treatment, and the setting of above-mentioned alkali process causes
Reaction site is discharged on polyester fiber strand；Meanwhile the present invention using limit 1000~5000Da relative molecular weights compared with
Fabric after small and integrated distribution fibroin albumen graft modification alkali process, is caused above-mentioned specific using covalently bound principle
The fibroin albumen of small-molecular-weight firmly introduces the fiber surface of polyester fabric, improves the nice and cool property of polyester fabric, flexibility, anti-
Static behaviour, while mechanical property is good, silk-fibroin stability is good.
Embodiment
The invention provides a kind of fibroin method of modifying of polyester fabric, including：
A) polyester fabric destarch is handled, obtains the fabric of destarch；
B the fabric of destarch) is used into naoh treatment, washs, obtains the fabric after alkali process；
C) fabric after alkali process is impregnated in cross-linking agent solution, mixed with the fibroin albumen of 1000~5000Da molecular weight
Close reaction.
The present invention, without limiting, well known to those skilled in the art can be defined as terylene for the polyester fabric
Fabric.The present invention, without limiting, can be commercially available for its source.
The present invention is first handled polyester fabric destarch, obtains the fabric of destarch.
In the present invention, the destarch processing is preferably handled using sodium carbonate destarch；It is preferred that it is specially that polyester fabric exists
The heated in water solution of sodium carbonate.The mode of the heating is preferably to boil heating.
The concentration of the sodium carbonate liquor is preferably 6g/L；The destarch treatment temperature is preferably 90~100 DEG C；It is described de-
It is preferably 1h to starch processing time.
After heating, preferably wash, be dehydrated, drying.
Washing of the present invention is preferably originally water washing, the present invention for the mode of washing without limiting, ability
Known to field technique personnel；The present invention for the dewatering type without limit, it is well known to those skilled in the art i.e.
Can；The present invention for the drying mode without limit, it is well known to those skilled in the art；Can be to dry or dry
It is dry.
The fabric of destarch is obtained after drying, the fabric of destarch is used into naoh treatment.
In the present invention, the mass concentration of the sodium hydroxide is preferably 5%~30%；The processing temperature of the sodium hydroxide
Degree is preferably 30~40 DEG C；The processing time of the sodium hydroxide is preferably 1~3h；More preferably 1~2h.
Source of the invention for the sodium hydroxide is commercially available without limiting.
After naoh treatment, preferably wash, dry.
Washing of the present invention is preferably originally water washing, the present invention for the mode of washing without limiting, ability
Known to field technique personnel；The present invention for the drying mode without limit, it is well known to those skilled in the art i.e.
Can；Can be to dry or dry.
After fabric washing, drying, the fabric after alkali process is obtained.
After the completion of alkali process, the fabric after alkali process is impregnated in cross-linking agent solution, with 1000~5000Da molecular weight
Fibroin albumen hybrid reaction, obtain the modified polyester fabric of silk-fibroin.
According to the present invention, the crosslinking agent includes carbodiimides；The crosslinking agent can also include N- acyl group succinyls
Imines；Wherein, the mass percent that the carbodiimides accounts for fibroin albumen is preferably 20%~40%；More preferably 25%.
The mass percent that the N- acylsuccinimides account for fibroin albumen is preferably 10%.
According to the present invention, after dipping, the fibroin albumen for adding the 1000~5000Da molecular weight being prepared is grafted
Reaction, final silk fibroin protein solution concentration is preferably 0.5-5%；
The reaction temperature is preferably 25~35 DEG C；The reaction time is preferably 1~3h.
After graft reaction, preferably dry, wash, dry again.
Drying of the present invention is preferably to dry；The washing is preferably water rinsing；Described dry again is preferably to dry.
The present invention for the concrete operations dried and rinse without restriction, it is well known to those skilled in the art.
In the present invention, the fibroin albumen is that relative molecular weight is smaller and the fibroin albumen of integrated distribution.
Fabric after the fibroin albumen graft modification alkali process of the small-molecular-weight using above-mentioned restriction of the invention,
The fibroin albumen of above-mentioned specific small-molecular-weight is caused firmly to introduce the fiber surface of polyester fabric using covalently bound principle,
Nice and cool property, flexibility, antistatic behaviour and the glossiness of polyester fabric are improved, improves the compatibility of skin, while mechanical property
Well, silk-fibroin stability is good.
The present invention for 1000~5000Da molecular weight fibroin albumen source without limit, can be it is commercially available,
It is preferred that prepare with the following method：
A) silkworm silk is subjected to degumming using sodium carbonate and then adds neutral salt solution dissolving, it is molten to prepare fibroin albumen
Solve liquid；
Glucan Sephadex G50 and G25 column equilibrations is stand-by；
B) fibroin albumen lysate is added in glucan Sephadex G50 posts, with sterilizing water elution fibroin albumen, divided
Pipe collects the silk fibroin water solution of desalination；
C) using the fibroin albumen of below 10kD in SDS-PAGE electrophoresis or mass spectrum screening step b) collecting pipes, add again
In glucan Sephadex G25 posts, with sterilizing water elution fibroin albumen, it is in charge of collection, screening obtains the small of 1000~5000Da
Molecular weight fibroin albumen.
Silkworm silk is carried out degumming by the present invention using sodium carbonate first；It is preferred that it is specially according to specific bath by silkworm silk
Than being put into aqueous sodium carbonate, handle, cleaning, be dried to obtain silk after degumming.
For the present invention for the silkworm silk without limiting, silkworm raw silk well known to those skilled in the art can
Think commercially available.
Bath raio of the present invention is preferably 1:50(g/mL)；The mass concentration of the aqueous sodium carbonate is preferably 0.2%；
The treatment temperature is preferably 98~100 DEG C；The number of processes is preferably 1~5 time；More preferably 2~3 times；It is described each
Processing time is preferably 30min.
Cleaned after being disposed, be dried to obtain silk after degumming.
It is of the present invention cleaning be preferably deionized water washing, the present invention for the cleaning way without limit, this
Known to art personnel；It is preferably well known to those skilled in the art pull loose after cleaning；The present invention is for described dry
Dry mode is well known to those skilled in the art without limiting；Can be to be dried in baking oven.The drying temperature is preferably
65℃。
By the silk after degumming and then neutral salt solution dissolving is added, prepares fibroin albumen lysate.
In the present invention, the neutral salt solution is preferably selected from lithium bromide or calcium chloride-ethanol；The silk and neutral salt
The bath raio of solution dissolving is preferably 1:15~1:20(g/mL)；
The concentration of the lithium-bromide solution is preferably 9~10M；In the calcium chloride-ethanol, mole of calcium chloride and ethanol
Than being preferably 1:2；
The dissolution time is 5~7 hours；The solution temperature is preferably 60 DEG C~70 DEG C；More preferably 65 DEG C~70
℃；The obtained fibroin albumen lysate concentration is preferably 10~50mg/mL.
Glucan Sephadex G50 and G25 column equilibrations is stand-by.The present invention is for the glucan Sephadex G50
Source with G25 posts can be commercially available without limiting, or self-control.
It is preferred that it is specially：The boiling of glucan Sephadex G50 and G25 sterile deionized water is boiled to abundant and expanded, continuously
Pour into the hollow column of glass material, standing sedimentation simultaneously balances column material with sterile deionized water；More preferably it is specially：Portugal is gathered
Sugared G-50 and G-25 are respectively put into deionized water, 1.5~2h of boiling water bath, are drained after normal temperature cooling with glass bar and are loaded glass
In the void column of material, natural subsidence is pressurizeed after 2~3 hours with peristaltic pump, the deionized water balance pillar of 5 times of column volumes three times.
The bed volume of the present invention for preparing is preferably 2cm × 60cm.
After balance, fibroin albumen lysate is added in glucan Sephadex G50 posts, with sterilizing water elution fibroin egg
In vain, it is in charge of the silk fibroin water solution for collecting desalination.
It is preferred that it is specially：Last time adds dissolving into glucan G-50 when equilibrium water drops to column material surface
Concentration be 10~50mg/mL fibroin albumen 5~15mL of mixed solution, when fibroin albumen mixed solution is completely into post material
When expecting surface, continue to be eluted with sterile deionized water, peristaltic pump control eluent flow rate, be in charge of collection efflux, until flowing to end
Fibroin albumen.
The elution flow rate is preferably 200~400mL/h；More preferably 250~350mL/h.
Using the fibroin albumen of below 10kD in SDS-PAGE electrophoresis or mass spectrum screening step b) collecting pipes.
Fibroin albumen of the present invention for below 10kD in the SDS-PAGE electrophoresis or mass spectrum screening step b) collecting pipes
Concrete mode without limit, it is well known to those skilled in the art.
In the present invention, also include after below 10kD fibroin albumen in the screening step b) collecting pipes lyophilized, then
It is configured to 30~80mg/mL aqueous solution.Then filter, filtering of the present invention is preferably the membrane filtration using 0.45 μm.
The present invention for the lyophilized concrete mode without limit, it is well known to those skilled in the art.
Using it is lyophilized then dissolve by the way of carry out below 10kD fibroin albumen molecular weight further separation.
After filtering, solution after filtering is added in glucan Sephadex G25 posts, with sterilizing water elution fibroin albumen, divided
Pipe is collected, and screening obtains 1000~5000Da small-molecular-weight fibroin albumen.
The flow velocity of elution of the present invention is preferably 30~70mL/h；More preferably 35~50mL/h.
After elution, using the small-molecular-weight fibroin egg of 1000~5000Da in SDS-PAGE electrophoresis or mass spectrum screening collecting pipe
In vain, then freeze and preserve.
The present invention can the easier small-molecular-weight fibroin egg for more accurately obtaining 1000~5000Da using aforesaid way
In vain.
The present invention for the screening and lyophilized concrete mode without limiting, it is well known to those skilled in the art i.e.
Can.
The invention provides a kind of fibroin method of modifying of polyester fabric, including：A) polyester fabric destarch is handled,
Obtain the fabric of destarch；B the fabric of destarch) is used into naoh treatment, washing and drying, obtains the fabric after alkali process；C)
Fabric after alkali process is impregnated in cross-linking agent solution, the fibroin albumen hybrid reaction with 1000~5000Da molecular weight.This
The fabric that the method for modifying that invention provides handles destarch uses naoh treatment, and the setting of above-mentioned alkali process make it that terylene is fine
Reaction site is discharged on dimension strand；Meanwhile the present invention is smaller using restriction 1000~5000Da relative molecular weights and collects
Fabric after the fibroin albumen graft modification alkali process of middle distribution, causes above-mentioned specified molecular weight using covalently bound principle
Fibroin albumen firmly introduces the fiber surface of polyester fabric, improve the nice and cool property of polyester fabric, flexibility, antistatic behaviour and
Glossiness, improve the compatibility of skin, while mechanical property is good, silk-fibroin stability is good.
It is the modified obtained polyester fabric soft surface of the present invention, smooth, refrigerant, there is soyeux skin compatibility,
Product is mainly used in high-grade underwear, the summer hangs out and the exploitation of bedding.
The mechanical property of fabric is measured present invention preferably employs following manner：
The polyester fabric that fibroin albumen is modified is cut into 15cm × 30cm sample, using material mechanical performance testing machine
Measure.
In order to further illustrate the present invention, the fibroin of polyester fabric provided by the invention is changed with reference to embodiments
Property method is described in detail.
Embodiment 1
(1) silkworm raw silk is pressed 1:50 (g/mL) bath raio is put into the aqueous sodium carbonate that concentration is 0.2%, in 98~
Silk three times, 30 minutes per treatment, is then fully cleaned up, pulled loose by 100 DEG C of processing with deionized water, is placed in baking oven and is done
It is dry, obtain the bombyx mori silk fibroin fiber after degumming.
(2) bombyx mori silk fibroin after degumming is weighed by 1:20 (g/mL) bath raio is dissolved in mol ratio 1:2 calcium chloride-ethanol
The aqueous solution in, 70 DEG C dissolving 5~7 hours bombyx mori silk fibroin dissolving mixed solution.
(3) glucan G-50 and G-25 are weighed to be respectively put into the deionized water of abundance, boiling water bath 1.5 hours, treats normal temperature
Drained and loaded in the void column of glass material with glass bar after cooling, prepare bed volume 2cm × 60cm, natural subsidence is after 2 hours
Pressurizeed with peristaltic pump, the deionized water balance pillar of 5 times of column volumes three times.
(4) for the last time when equilibrium water drops to column material surface, the fibroin egg of dissolving is added into glucan G-50
White 5~15ml of mixed solution (10~50mg/ml), when fibroin albumen mixed solution is completely into column material surface, continue
Eluted with sterile deionized water, peristaltic pump control eluent flow rate 300mL/h；It is in charge of collection efflux, until flowing to end fibroin egg
In vain.
(5) using the molecular weight distribution of SDS-PAGE electrophoresis or each pipe of Mass Spectrometric Identification, by fibroin of the molecular weight less than 10kDa
Protein solution is freeze-dried mixed, is then configured to the 30~80mg/ml aqueous solution, and microparticle is removed with 0.45 μm of membrane filtration.
(6) similar step (4), the silk fibroin water solution of appropriate step (5), peristaltic pump control are added to G-25 column material
Eluent flow rate 50mL/h processed, is in charge of collection efflux, until flowing to end fibroin albumen, obtains the silk of 1000~5000Da molecular weight
The fibroin aqueous solution, finally freeze and preserve.
(7) weaving polyester fabric is boiled with 6g/1L aqueous sodium carbonate at 100 DEG C and boiled 1 hour or so, taken out with certainly
Water repeatedly dry by washes clean, dehydration.Then with handling polyester fabric 1~2 hour at 10% 30 DEG C of sodium hydroxide solution,
Be impregnated in after tap water rinse is clean certain mass concentration carbodiimides and n-hydroxysuccinimide (quality respectively may be about
Add the 25% of fibroin albumen quality, in mixed aqueous solution 10%), impregnate 1 hour or so, add the fibroin that step 6 obtains
After protein dry powder (fibroin albumen final mass concentration is 0.5%) dissolving, 3h is reacted, taking-up is dried, rinsed, drying again.
(8) polyester fabric that fibroin albumen is modified is cut into 15cm × 30cm sample, tried using material mechanical performance
The machine of testing measures 95% or so that fracture strength is original fabric, and elongation at break is 97% or so of original fabric.
(9) the fibroin albumen rate of body weight gain 3.1% on polyester fabric is measured using weight method, is significantly higher than the increasing of comparative example 1
Rate 0.9% again.The fabric that fibroin albumen is modified is impregnated in after being vibrated 24 hours in 37 DEG C of warm water, measures the molten of fibroin albumen
Mistake rate is less than 5%.
Embodiment 2
(1) silkworm raw silk is pressed 1:50 (g/mL) bath raio is put into the aqueous sodium carbonate that concentration is 0.2%, in 98~
Silk three times, 30 minutes per treatment, is then fully cleaned up, pulled loose by 100 DEG C of processing with deionized water, is placed in baking oven and is done
It is dry, obtain the bombyx mori silk fibroin fiber after degumming.
(2) bombyx mori silk fibroin after degumming is weighed by 1:20 (g/mL) bath raio is dissolved in mol ratio 1:2 calcium chloride-ethanol
The aqueous solution in, 70 DEG C dissolving 5~7 hours bombyx mori silk fibroin dissolving mixed solution.
(3) glucan G-50 and G-25 are weighed to be respectively put into the deionized water of abundance, boiling water bath 1.5 hours, treats normal temperature
Drained and loaded in the void column of glass material with glass bar after cooling, prepare bed volume 2cm × 60cm, natural subsidence is after 2 hours
Pressurizeed with peristaltic pump, the deionized water balance pillar of 5 times of column volumes three times.
(4) for the last time when equilibrium water drops to column material surface, the fibroin egg of dissolving is added into glucan G-50
White 5~15ml of mixed solution (10~50mg/ml), when fibroin albumen mixed solution is completely into column material surface, continue
Eluted with sterile deionized water, peristaltic pump control eluent flow rate 300mL/h；It is in charge of collection efflux, until flowing to end fibroin egg
In vain.
(5) using the molecular weight distribution of SDS-PAGE electrophoresis or each pipe of Mass Spectrometric Identification, by fibroin of the molecular weight less than 10kDa
Protein solution is freeze-dried mixed, is then configured to the 30~80mg/ml aqueous solution, and microparticle is removed with 0.45 μm of membrane filtration.
(6) similar step (4), the silk fibroin water solution of appropriate step (5), peristaltic pump control are added to G-25 column material
Eluent flow rate 50mL/h processed, is in charge of collection efflux, until flowing to end fibroin albumen, obtains the silk of 1000~5000Da molecular weight
The fibroin aqueous solution, finally freeze and preserve.
(7) weaving polyester fabric is boiled with 6g/1L aqueous sodium carbonate at 100 DEG C and boiled 1 hour or so, taken out with certainly
Water repeatedly dry by washes clean, dehydration.Then with handling polyester fabric 2~3 hours at 10% 30 DEG C of sodium hydroxide solution,
Be impregnated in after tap water rinse is clean certain mass concentration carbodiimides and n-hydroxysuccinimide (quality respectively may be about
Add the 25% of fibroin albumen quality, in mixed aqueous solution 10%), impregnate 1 hour or so, add the fibroin that step 6 obtains
After protein dry powder (fibroin albumen final mass concentration is 1%) dissolving, 3h is reacted, taking-up is dried, rinsed, drying again.
(8) polyester fabric that fibroin albumen is modified is cut into 15cm × 30cm sample, tried using material mechanical performance
The machine of testing measures 91% or so that fracture strength is original fabric, and elongation at break is 92% or so of original fabric.
(9) the fibroin albumen rate of body weight gain 5.8% on polyester fabric is measured using weight method, is significantly higher than the increasing of comparative example 2
Rate 0.92% again.The fabric that fibroin albumen is modified is impregnated in after being vibrated 24 hours in 37 DEG C of warm water, measures fibroin albumen
Dissolve-loss ratio is less than 8%.
Embodiment 3
(1) silkworm raw silk is pressed 1:50 (g/mL) bath raio is put into the aqueous sodium carbonate that concentration is 0.2%, in 98~
Silk three times, 30 minutes per treatment, is then fully cleaned up, pulled loose by 100 DEG C of processing with deionized water, is placed in baking oven and is done
It is dry, obtain the bombyx mori silk fibroin fiber after degumming.
(2) bombyx mori silk fibroin after degumming is weighed by 1:20 (g/mL) bath raio is dissolved in mol ratio 1:2 calcium chloride-ethanol
The aqueous solution in, 70 DEG C dissolving 5~7 hours bombyx mori silk fibroin dissolving mixed solution.
(3) glucan G-50 and G-25 are weighed to be respectively put into the deionized water of abundance, boiling water bath 1.5 hours, treats normal temperature
Drained and loaded in the void column of glass material with glass bar after cooling, prepare bed volume 2cm × 60cm, natural subsidence is after 2 hours
Pressurizeed with peristaltic pump, the deionized water balance pillar of 5 times of column volumes three times.
(4) for the last time when equilibrium water drops to column material surface, the fibroin egg of dissolving is added into glucan G-50
White 5~15ml of mixed solution (10~50mg/ml), when fibroin albumen mixed solution is completely into column material surface, continue
Eluted with sterile deionized water, peristaltic pump control eluent flow rate 300mL/h；It is in charge of collection efflux, until flowing to end fibroin egg
In vain.
(5) using the molecular weight distribution of SDS-PAGE electrophoresis or each pipe of Mass Spectrometric Identification, by fibroin of the molecular weight less than 10kDa
Protein solution is freeze-dried mixed, is then configured to the 30~80mg/ml aqueous solution, and microparticle is removed with 0.45 μm of membrane filtration.
(6) similar step (4), the silk fibroin water solution of appropriate step (5), peristaltic pump control are added to G-25 column material
Eluent flow rate 50mL/h processed, is in charge of collection efflux, until flowing to end fibroin albumen, obtains the silk of 1000~5000Da molecular weight
The fibroin aqueous solution, finally freeze and preserve.
(7) weaving polyester fabric is boiled with 6g/1L aqueous sodium carbonate at 100 DEG C and boiled 1 hour or so, taken out with certainly
Water repeatedly dry by washes clean, dehydration.Then with handling polyester fabric 1~2 hour at 10% 30 DEG C of sodium hydroxide solution,
Be impregnated in after tap water rinse is clean certain mass concentration carbodiimides and n-hydroxysuccinimide (quality respectively may be about
Add the 25% of fibroin albumen quality, in mixed aqueous solution 10%), impregnate 1 hour or so, add the fibroin that step 6 obtains
After protein dry powder (fibroin albumen final mass concentration is 1%) dissolving, 3h is reacted, taking-up is dried, rinsed, drying again.
(8) polyester fabric that fibroin albumen is modified is cut into 15cm × 30cm sample, tried using material mechanical performance
The machine of testing measures 97% or so that fracture strength is original fabric, and elongation at break is 97% or so of original fabric.
(9) the fibroin albumen rate of body weight gain 5.2% on polyester fabric is measured using weight method, is significantly higher than the increasing of comparative example 2
Rate 0.92% again.The fabric that fibroin albumen is modified is impregnated in after being vibrated 24 hours in 37 DEG C of warm water, measures fibroin albumen
Dissolve-loss ratio is less than 8%.
Comparative example 1
(1) silkworm raw silk is pressed 1:50 (g/mL) bath raio is put into the aqueous sodium carbonate that concentration is 0.2%, in 98~
Silk three times, 30 minutes per treatment, is then fully cleaned up, pulled loose by 100 DEG C of processing with deionized water, is placed in baking oven and is done
It is dry, obtain the bombyx mori silk fibroin fiber after degumming.
(2) bombyx mori silk fibroin after degumming is weighed by 1:20 (g/mL) bath raio is dissolved in mol ratio 1:2 calcium chloride-ethanol
The aqueous solution in, 70 DEG C dissolving 5~7 hours bombyx mori silk fibroin dissolving mixed solution.
(3) glucan G-50 and G-25 are weighed to be respectively put into the deionized water of abundance, boiling water bath 1.5 hours, treats normal temperature
Drained and loaded in the void column of glass material with glass bar after cooling, prepare bed volume 2cm × 60cm, natural subsidence is after 2 hours
Pressurizeed with peristaltic pump, the deionized water balance pillar of 5 times of column volumes three times.
(4) for the last time when equilibrium water drops to column material surface, the fibroin egg of dissolving is added into glucan G-50
White 5~15ml of mixed solution (10~50mg/ml), when fibroin albumen mixed solution is completely into column material surface, continue
Eluted with sterile deionized water, peristaltic pump control eluent flow rate 300mL/h；It is in charge of collection efflux, until flowing to end fibroin egg
In vain.
(5) using the molecular weight distribution of SDS-PAGE electrophoresis or each pipe of Mass Spectrometric Identification, by fibroin of the molecular weight less than 10kDa
Protein solution is freeze-dried mixed, is then configured to the 30~80mg/ml aqueous solution, and microparticle is removed with 0.45 μm of membrane filtration.
(6) similar step (4), the silk fibroin water solution of appropriate step (5), peristaltic pump control are added to G-25 column material
Eluent flow rate 50mL/h processed, is in charge of collection efflux, until flowing to end fibroin albumen, obtains the silk of 1000~5000Da molecular weight
The fibroin aqueous solution, finally freeze and preserve.
(7) weaving polyester fabric is boiled with 6g/1L aqueous sodium carbonate at 100 DEG C and boiled 1 hour or so, taken out with certainly
Water repeatedly after washes clean, be impregnated in the aqueous solution for the fibroin albumen that the step 6 that mass concentration is 0.5% obtains, room temperature
Continuous action and the same time of embodiment 1~4, taking-up are dried, rinsed, drying again.
(8) the fibroin albumen rate of body weight gain 0.9% on polyester fabric, the hot water dissolve-loss ratio of fibroin albumen are measured using weight method
About 25%.
Comparative example 2
(1) silkworm raw silk is pressed 1:50 (g/mL) bath raio is put into the aqueous sodium carbonate that concentration is 0.2%, in 98~
Silk three times, 30 minutes per treatment, is then fully cleaned up, pulled loose by 100 DEG C of processing with deionized water, is placed in baking oven and is done
It is dry, obtain the bombyx mori silk fibroin fiber after degumming.
(2) bombyx mori silk fibroin after degumming is weighed by 1:20 (g/mL) bath raio is dissolved in mol ratio 1:2 calcium chloride-ethanol
The aqueous solution in, 70 DEG C dissolving 5~7 hours bombyx mori silk fibroin dissolving mixed solution.
(3) glucan G-50 and G-25 are weighed to be respectively put into the deionized water of abundance, boiling water bath 1.5 hours, treats normal temperature
Drained and loaded in the void column of glass material with glass bar after cooling, prepare bed volume 2cm × 60cm, natural subsidence is after 2 hours
Pressurizeed with peristaltic pump, the deionized water balance pillar of 5 times of column volumes three times.
(4) for the last time when equilibrium water drops to column material surface, the fibroin egg of dissolving is added into glucan G-50
White 5~15ml of mixed solution (10~50mg/ml), when fibroin albumen mixed solution is completely into column material surface, continue
Eluted with sterile deionized water, peristaltic pump control eluent flow rate 300mL/h；It is in charge of collection efflux, until flowing to end fibroin egg
In vain.
(5) using the molecular weight distribution of SDS-PAGE electrophoresis or each pipe of Mass Spectrometric Identification, by fibroin of the molecular weight less than 10kDa
Protein solution is freeze-dried mixed, is then configured to the 30~80mg/ml aqueous solution, and microparticle is removed with 0.45 μm of membrane filtration.
(6) similar step (4), the silk fibroin water solution of appropriate step (5), peristaltic pump control are added to G-25 column material
Eluent flow rate 50mL/h processed, is in charge of collection efflux, until flowing to end fibroin albumen, obtains the silk of 1000~5000Da molecular weight
The fibroin aqueous solution, finally freeze and preserve.
(7) weaving polyester fabric is boiled with 6g/1L aqueous sodium carbonate at 100 DEG C and boiled 1 hour or so, taken out with certainly
Water repeatedly after washes clean, be impregnated in the aqueous solution for the fibroin albumen that the step 6 that mass concentration is 1% obtains, room temperature is held
Continuous effect and the same time of embodiment 1~4, taking-up are dried, rinsed, drying again.
(8) the fibroin albumen rate of body weight gain 0.92% on polyester fabric, the molten mistake of hot water of fibroin albumen are measured using weight method
Rate about 25%.
Comparative example 3
(1) silkworm raw silk is pressed 1:50 (g/mL) bath raio is put into the aqueous sodium carbonate that concentration is 0.2%, in 98~
Silk three times, 30 minutes per treatment, is then fully cleaned up, pulled loose by 100 DEG C of processing with deionized water, is placed in baking oven and is done
It is dry, obtain the bombyx mori silk fibroin fiber after degumming.
(2) bombyx mori silk fibroin after degumming is weighed by 1:15 (g/mL) bath raio is dissolved in 9.3M lithium bromide water solutions, and 65
DEG C dissolving 5~7 hours bombyx mori silk fibroin dissolving mixed solution.
(3) bombyx mori silk fibroin lysate being poured into bag filter, bag filter wall is pellicle, molecular cut off is 12.0~
16.0kDa scopes, the bag filter for being filled with bombyx mori silk fibroin lysate is placed in the container for filling deionized water, every 2 hours
The water in container is changed with new deionized water or pure water, persistently dialyses 3 days, obtains the silk fibroin protein aqueous solution after purification
(preparation of conventional silk fibroin water solution), then freeze and preserve.
(4) weaving polyester fabric is boiled 1 hour or so with 6g/1L aqueous sodium carbonate boiling, taking-up originally water washing,
It is dehydrated, dries or dries, then with polyester fabric is handled at 10% 30 DEG C of sodium hydroxide solution 1~2 hour, is impregnated after rinsing
In the carbodiimides and n-hydroxysuccinimide of certain mass concentration, (quality, which respectively may be about, adds fibroin albumen quality
25%th, in mixed aqueous solution 10%), impregnate 1 hour or so, add lyophilized fibroin albumen (the final matter of fibroin albumen of step 3
After concentration is measured 1%) to dissolve, 3h is reacted, taking-up is dried, rinsed, drying again.
(5) polyester fabric that fibroin albumen is modified is cut into 15cm × 30cm sample, tried using material mechanical performance
The machine of testing measures 94% or so that fracture strength is original fabric, and elongation at break is 97% or so of original fabric.
(6) the fibroin albumen rate of body weight gain 1.4% on polyester fabric, the fabric that fibroin albumen is modified are measured using weight method
It is impregnated in after being vibrated 24 hours in 37 DEG C of warm water, the dissolve-loss ratio for measuring fibroin albumen is less than 10%.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
- A kind of 1. fibroin method of modifying of polyester fabric, it is characterised in that including：A) polyester fabric destarch is handled, obtains the fabric of destarch；B the fabric of destarch) is used into naoh treatment, washs, obtains the fabric after alkali process；C) fabric after alkali process is impregnated in cross-linking agent solution, mixed instead with the fibroin albumen of 1000~5000Da molecular weight Should.
- 2. according to the method for claim 1, it is characterised in that the crosslinking agent includes carbodiimides.
- 3. according to the method for claim 2, it is characterised in that the carbodiimides accounts for the mass percent of fibroin albumen For 20%~40%.
- 4. the destarch is specially using at sodium carbonate destarch according to the method for claim 1, it is characterised in that step A) Reason；The concentration of the sodium carbonate liquor is 6g/L；The destarch treatment temperature is 90~100 DEG C；The destarch processing time is 1h。
- 5. according to the method for claim 1, it is characterised in that step B) mass concentration of the sodium hydroxide for 5%~ 30%；The treatment temperature of the sodium hydroxide is 30~40 DEG C；The processing time of the sodium hydroxide is 1~3h.
- 6. the reaction temperature is 25~35 DEG C according to the method for claim 1, it is characterised in that step C)；It is described anti- It is 1~3h between seasonable.
- 7. according to the method for claim 1, it is characterised in that the fibroin albumen of 1000~5000Da molecular weight according to It is prepared by following method：A) silkworm silk is subjected to degumming using sodium carbonate and then adds neutral salt solution dissolving, prepare fibroin albumen lysate；Glucan Sephadex G50 and G25 column equilibrations is stand-by；B) fibroin albumen lysate is added in glucan Sephadex G50 posts, with sterilizing water elution fibroin albumen, is in charge of receipts Collect the silk fibroin water solution of desalination；C) using the fibroin albumen of below 10kD in SDS-PAGE electrophoresis or mass spectrum screening step b) collecting pipes, Portugal is added again and is gathered In sugared Sephadex G25 posts, with sterilizing water elution fibroin albumen, it is in charge of collection, screening obtains 1000~5000Da small molecule Measure fibroin albumen.
- 8. according to the method for claim 7, it is characterised in that the step a) neutral salt solutions are selected from lithium bromide or chlorination Calcium-ethanol；The dissolution time is 5~7 hours；The fibroin albumen lysate concentration is 10~50mg/mL.
- 9. according to the method for claim 7, it is characterised in that below 10kD in step c) the screening step b) collecting pipes Fibroin albumen after also include it is lyophilized, be then configured to 30~80mg/mL aqueous solution.
- 10. according to the method for claim 7, it is characterised in that the step b) elution flow rates are 200~400mL/h；Step Rapid c) described elution flow rate is 30~70mL/h.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108642878A (en) * | 2018-04-19 | 2018-10-12 | 中原工学院 | A method of arranging polyester fabric using fibroin albumen and gamma-polyglutamic acid self-assembly |
| CN111793981A (en) * | 2020-07-10 | 2020-10-20 | 温州厚德服饰有限公司 | Moisture permeable cotton-padded clothes |
| CN112853748A (en) * | 2020-12-23 | 2021-05-28 | 南通雅文纺织品有限公司 | Production process of soft fabric for quilt cover |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000328455A (en) * | 1999-05-25 | 2000-11-28 | Sekisui Chem Co Ltd | Fiber treating agent |
| CN1920162A (en) * | 2006-09-15 | 2007-02-28 | 浙江大学 | Method of coating polyester piece goods by silk fibroin combination liquid |
-
2017
- 2017-11-29 CN CN201711224540.8A patent/CN107869046B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000328455A (en) * | 1999-05-25 | 2000-11-28 | Sekisui Chem Co Ltd | Fiber treating agent |
| CN1920162A (en) * | 2006-09-15 | 2007-02-28 | 浙江大学 | Method of coating polyester piece goods by silk fibroin combination liquid |
Non-Patent Citations (2)
| Title |
|---|
| 杜孟芳: ""蚕丝蛋白涂复涤纶织物的研究"", 《中国博士学位论文全文数据库 工程科技I辑》 * |
| 汤晓: "《蛋白质核酸类药物生产与分析技术》", 31 May 2012, 宁波出版社 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108642878A (en) * | 2018-04-19 | 2018-10-12 | 中原工学院 | A method of arranging polyester fabric using fibroin albumen and gamma-polyglutamic acid self-assembly |
| CN108642878B (en) * | 2018-04-19 | 2020-11-27 | 中原工学院 | Method for finishing polyester fabric by using silk fibroin and gamma-polyglutamic acid self-assembly |
| CN111793981A (en) * | 2020-07-10 | 2020-10-20 | 温州厚德服饰有限公司 | Moisture permeable cotton-padded clothes |
| CN112853748A (en) * | 2020-12-23 | 2021-05-28 | 南通雅文纺织品有限公司 | Production process of soft fabric for quilt cover |
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