CN107868749B

CN107868749B - Culture dish device of uncapping and ames tester

Info

Publication number: CN107868749B
Application number: CN201610853349.9A
Authority: CN
Inventors: 郑劲林; 杨富成; 李朝文; 刘玉杰; 赵银龙
Original assignee: Beijing Huironghe Technology Co Ltd
Current assignee: Beijing Huironghe Technology Co Ltd
Priority date: 2016-09-26
Filing date: 2016-09-26
Publication date: 2022-11-22
Anticipated expiration: 2036-09-26
Also published as: CN107868749A

Abstract

The invention provides a culture dish cover opening and closing device and an Ames tester. The culture dish device of opening and shutting includes: a support; an upper plate fixedly or rotatably mounted to the bracket and having at least one first stepped hole thereon; the station turntable is fixedly or rotatably arranged on the bracket and is positioned below the upper disc, and at least one second step hole is formed in the station turntable; and a lifting mechanism having a carrying section. The culture dish cover opening and closing device and the Ames tester are provided with the upper plate, the station turntable and the lifting mechanism, so that the dish cover of each culture dish can be separated from the dish plate, and the separated dish cover can be combined with the dish plate. The station rotating disc can also convey the dish to each station so as to add various substances to the dish and the like.

Description

Culture dish device of uncapping and ames tester

Technical Field

The invention relates to the technical field of Ames tests, in particular to a culture dish cover opening and closing device and an Ames tester with the culture dish cover opening and closing device.

Background

Through more than ten years of effort, the established and constantly developed and developed salmonella back-mutation test (also called Ames test and Ames test) in 1975 has been widely adopted by countries in the world. The method is rapid, simple, sensitive and economical, is suitable for testing mixtures, and reflects the comprehensive effect of various pollutants. The mutagenicity of food additives, cosmetics and the like can be detected by Ames test, and thus the carcinogenicity can be presumed; the Ames test is used for detecting the mutagenicity of water source water and drinking water, and a more sanitary and safer disinfection measure is explored compared with the existing method; or detecting the mutagenicity of the urban sewage and the industrial wastewater, combining chemical analysis, tracking pollution sources and providing basis for researching prevention and control strategies; detecting the mutagenicity of soil, sludge, industrial waste residue compost and waste ash to prevent the soil for maintaining life from being polluted by mutagenic substances and damaging human beings by crops; detecting the mutagenicity of gaseous pollutants, and preventing the pollutants from generating potential harm to human bodies through breathing through the atmosphere; the Ames test is used for researching the relation between the structure of the compound and the denaturation, and theoretical basis is provided for synthesizing a new compound which has no potential harm to the environment; the mutagenicity of the pesticide before and after microbial degradation is detected, and whether the pesticide has hidden danger to human beings in the metabolism process after application is known; also, screening of antimutators using the Ames assay, research and development of new anticancer drugs, and the like. The existing Ames test is that the laboratory staff adopts a dropper to add reagent into a culture dish, the automation degree is low, the labor cost is high, certain experimental precision can be guaranteed, and the deviation of the experimental result is huge due to human errors.

Disclosure of Invention

The invention aims to provide a culture dish cover opening and closing device which can separate a dish plate and a dish cover of each culture dish, and can combine the dish plate and the dish cover containing various substances again after various substances are added into the dish plate so as to provide support for realizing a full-automatic Ames tester.

To this end, the invention provides a culture dish cover opening and closing device for an Ames tester, comprising:

a support;

an upper plate fixedly or rotatably mounted to the bracket and having at least one first stepped hole thereon;

the station turntable is fixedly or rotatably arranged on the bracket and is positioned below the upper disc, and at least one second step hole is formed in the station turntable; and

an elevating mechanism having a carrying portion, the elevating mechanism being configured to:

when one first step hole and one second step hole are positioned at the same axial position, the carrying part carrying the culture dish moves downwards from the upper part of the upper dish, so that the carrying part sequentially passes through the first step hole and the second step hole, and in the moving process of the carrying part, the dish cover of the culture dish is positioned in the first step hole and the dish plate of the culture dish is positioned in the second step hole, so that the dish cover of the culture dish is separated from the dish plate; and

when the first step hole for carrying the dish cover and the second step hole for carrying the dish plate are positioned at the same axial position, the supporting part is moved upwards from the lower part of the station turntable, so that the supporting part sequentially passes through the second step hole and the first step hole, and in the process of moving the supporting part, the dish plate of the culture dish is moved upwards to be matched with the dish cover of the culture dish, so that the dish cover of the culture dish is arranged on the dish plate.

Further, the station carousel is rotatably mounted to the frame and is configured to transport a dish tray located in the second stepped bore to each predetermined station.

Further, the number of the first stepped holes is multiple, and the number of the first stepped holes is equal to that of the second stepped holes;

the station turntable and the upper disc are rotatably mounted on the support, and the station turntable and the upper disc synchronously rotate.

Further, the culture dish opening and closing device further comprises a rotating device which is arranged below the station turntable and is configured to drive the station turntable to rotate.

Further, the culture dish opening and closing device further comprises a linkage mechanism which is configured to enable the station turntable and the upper disk to synchronously rotate.

Further, the diameter of the station turntable is larger than that of the upper disc.

Further, elevating system still includes sharp slip table to drive the portion of carrying reciprocates.

Further, the carrying part is a bracket or a tray.

The invention also provides an Ames tester which comprises any culture dish cover opening and closing device, wherein the culture dish cover opening and closing device is configured to separate a dish cover of a culture dish from a dish plate, and the dish cover is arranged on the dish plate after mixed liquid is added into the dish plate.

Further, the station carousel of the culture dish opening and closing cover device is configured to drive the dish from the dish lid opening and closing station to the mixed liquid adding station, and after the mixed liquid is added in the dish, the dish is driven from the mixed liquid adding station to the dish lid opening and closing station.

The culture dish cover opening and closing device and the Ames tester are provided with the upper plate, the station turntable and the lifting mechanism, so that the dish cover of each culture dish can be separated from the dish plate, and the separated dish cover can be combined with the dish plate. The station rotating disc can also convey the dish to each station so as to add various substances to the dish and the like.

Drawings

Some specific embodiments of the invention will be described in detail hereinafter, by way of illustration and not limitation, with reference to the accompanying drawings. The same reference numbers in the drawings identify the same or similar elements or components. Those skilled in the art will appreciate that the drawings are not necessarily drawn to scale. In the drawings:

FIG. 1 is a schematic block diagram of a petri dish lid opening and closing device according to an embodiment of the invention;

FIG. 2 is a schematic block diagram of an Ames tester according to one embodiment of the present invention;

FIG. 3 is a schematic partial block diagram of an Ames tester according to one embodiment of the present invention;

FIG. 4 is a schematic partial block diagram of an Ames tester according to one embodiment of the present invention;

FIG. 5 is a schematic partial block diagram of an Ames tester according to one embodiment of the present invention.

Detailed Description

To make the objects, technical solutions and advantages of the embodiments of the present invention clearer, specific embodiments will be described in detail below with reference to the accompanying drawings. It should be noted that in the embodiments of the present invention, some of the pipelines are only shown in the figures as partial pipe sections, and some of the pipelines are not shown in the figures.

Fig. 1 is a schematic block diagram of a culture dish opening and closing device 300 according to an embodiment of the present invention, and as shown in fig. 1, the embodiment of the present invention provides a culture dish opening and closing device 300 for an emms tester. The empty culture dish opening and closing device 300 may include a rack, an upper plate 310, a station turntable 320, and a lifting mechanism 330.

The upper plate 310 is fixedly or rotatably mounted to the bracket and has at least one first stepped hole 311.

The station turntable 320 is fixedly or rotatably mounted to the bracket and is positioned below the upper plate 310, and the station turntable 320 has at least one second stepped hole 321.

The lifting mechanism 330 may have a carrying portion 331, and the lifting mechanism 330 is configured to:

when one first stepped hole 311 and one second stepped hole 321 are positioned at the same axial position, the carrying part 331 carrying the culture dish is moved downwards from the upper part of the upper disk 310, so that the carrying part 331 sequentially passes through the first stepped hole 311 and the second stepped hole 321, and in the moving process of the carrying part 331, the dish cover 720 of the culture dish is positioned at the first stepped hole 311 and the dish plate 710 of the culture dish is positioned at the second stepped hole 321, so that the dish cover 720 of the culture dish is separated from the dish plate 710; and

when the first stepped hole 311 of one carrying dish cover 720 and the second stepped hole 321 of one carrying dish plate 710 are in the same axial position, the carrying part 331 is moved upward from the lower side of the station turntable 320, so that the carrying part 331 sequentially passes through the second stepped hole 321 and the first stepped hole 311, and in the moving process of the carrying part 331, the dish plate of the culture dish is moved upward to be matched with the dish cover of the culture dish, so that the dish cover of the culture dish is mounted on the dish plate.

Specifically, when the dish plate and the dish lid of the culture dish need to be separated, one first stepped hole 311 and one second stepped hole 321 are positioned coaxially and above the carrying portion 331. Then, the carrying portion 331 is raised above the upper tray 310, and a culture dish is manually placed on the carrying portion 331 from another device or by hand. Then, the lifting mechanism 330 drives the culture dish to descend, so that the dish cover is positioned on the step surface of the first step hole 311, and the dish tray is positioned on the step surface of the second step hole 321. Similarly, when the dish at the second stepped hole 321 and the dish at the first stepped hole 311 need to be combined, the lifting mechanism 330 may drive the carrying portion 331 to perform a lifting motion. The culture dish opening and closing device 300 in this embodiment can quickly separate the dish tray and the dish lid of the culture dish, or make the dish lid set in the dish tray.

In some embodiments of the present invention, the number of the first stepped holes 311 and the second stepped holes 321 should be equal. When the upper plate 310 has only one first stepped hole 311 and the station turntable 320 has only one second stepped hole 321, the upper plate 310 may be fixed to the bracket and the station turntable 320 may be fixed to and also rotatably mounted to the bracket. The reason for this is that, when the first stepped hole 311 and the second stepped hole 321 are both one, the dish lid opening and closing station and the mixed liquid addition station may be at the same position or different positions. The station turntable 320 may be fixed to the rack when at the same station, and the station turntable 320 may be configured to be rotatably mounted to the rack when not at a station. Alternatively, the upper disc 310 may also be rotatably mounted to a stand and rotate in synchronism with the station carousel 320 to ensure that the first and

second step holes

311, 321 are coaxial at the disc closure opening and closing station.

In other embodiments of the present invention, the number of the first stepped holes 311 is multiple, and the number of the first stepped holes 311 is equal to the number of the second stepped holes 321. The station turntable 320 and the upper disk 310 are rotatably mounted on the support, and the station turntable 320 and the upper disk 310 rotate synchronously to ensure the synchronism of the dish and the dish cover of the same culture dish. Under the condition, the opening and closing station of the dish plate and the mixing liquid adding station can be arranged at different stations, so that when substances are added, the other culture dish can be separated or combined with the dish plate and the dish cover.

Preferably, in both of the above embodiments, the station carousel 320 is configured to transport a dish located within the second stepped bore 321 to each predetermined station when the station carousel 320 is rotatably mounted to the rack.

In some embodiments of the present invention, the station turntable 320 and the upper plate 310 are rotatably mounted on the frame, and when the station turntable 320 and the upper plate 310 rotate synchronously, the culture dish opening and closing device 300 in this embodiment further comprises a rotating device and a linkage mechanism. The rotating device is disposed below the station turntable 320 and configured to drive the station turntable 320 to rotate. The linkage is configured to rotate the station carousel 320 and the upper plate 310 in synchronization.

Preferably, the diameter of the station carousel 320 is larger than the upper disk 310 to prevent inconvenience caused by the upper disk 310 blocking the station carousel 320 from adding a mixed solution of multiple substances into the dish. The rotating shaft of the station turntable 320 and the rotating shaft of the upper plate 310 are arranged in parallel at intervals, and it is ensured that when the dish cover is opened and closed at a station, one first step hole 311 and one second step hole 321 are arranged coaxially.

In some embodiments of the present invention, the lifting mechanism 330 further includes a linear sliding table to move the supporting portion 331 up and down. The carrying portion 331 is preferably a tray or a tray.

Fig. 2 is a schematic structural diagram of an emms tester according to an embodiment of the present invention, and as shown in fig. 2 and referring to fig. 3 to fig. 5, an embodiment of the present invention further provides an emms tester, which may include a base 100, a culture dish storage and sorting device 200, a culture dish cover opening and closing device 300, and a sample adding system 400 in any of the above embodiments, so that the emms test may be automated, and the test efficiency and the test accuracy may be improved on the basis of reducing the labor cost.

The culture dish storing and sorting device 200 is configured to take out the culture dishes stored in the culture dish storing and sorting device 200 without adding a plurality of substances one by using the lifting mechanism 330 of the culture dish opening and closing device 300, and store the culture dishes in the culture dish storing and sorting device 200 after adding a mixed liquid composed of a plurality of substances to each culture dish.

The culture dish opening and closing device 300 is configured to separate a dish cover 720 and a dish plate 710 of a culture dish, and after mixed liquid is added into the dish plate, the dish cover is installed on the dish plate.

The sample addition system 400 can be configured to mix multiple solutions with a sample and deliver the mixed solution formed after mixing into a dish on the petri dish lid-opening device 300.

In some embodiments of the present invention, the culture dish storage and sorting device 200 may include a base plate 210 and a culture dish rack 220.

The bottom plate 210 may be a circular plate and is mounted on the base 100, and has a through hole 211, and the size of the through hole 211 may be slightly larger or larger than the diameter of the culture dish to allow the culture dish to pass through.

The dish holder 220 is rotatably mounted to the base plate 210. The culture dish holder 220 may have a plurality of accommodating chambers arranged in the circumferential direction of the bottom plate 210, each accommodating chamber extending in the vertical direction, and each accommodating chamber being coaxial with the through hole 211 when rotated above the through hole 211. A stack of petri dishes can be placed in each receiving chamber.

The lift mechanism 330 of the petri dish lid opening and closing device 300 can be configured to:

firstly, the carrying part 331 moves upwards into the through hole 211, so that a culture dish which rotates to a containing cavity coaxial with the through hole 211 and containing at least one culture dish is positioned on the carrying part 331, then the carrying part 331 moves downwards, and the upper surface of the lowest culture dish in the containing cavity moves to a position which is flush with the upper surface of the bottom plate 210 or lower than the upper surface of the bottom plate 210, so that the lowest culture dish in the containing cavity is sorted out; and/or

The tray portion 331 carrying the one or more culture dishes is moved upward, and the lower surface of the lowermost one of the one or more culture dishes is moved to a position flush with the upper surface of the bottom plate 210 or higher than the upper surface of the bottom plate 210, so that the one or more culture dishes are stored in the receiving chamber rotated to be coaxial with the through-hole 211.

In some implementations of embodiments of the present invention, the plurality of receiving cavities includes at least one sorting receiving cavity each configured to store a culture dish to be sorted and at least one storage receiving cavity each configured to store a culture dish to be stored.

During the specific use, place a pile of culture dish at first in every letter sorting holds the intracavity, every culture dish has ware lid and ware dish. When the culture dishes in one sorting and accommodating chamber need to be taken out one by one, the carrying part 331 is moved upwards into the through hole 211, so that the upper surface of the carrying part 331 is approximately flush with the upper surface of the bottom plate 210. The culture dish holder 220 is rotated, so that the sorting accommodating cavity is rotated to the upper side of the through hole 211, and due to the existence of the supporting part 331, the culture dish in the sorting accommodating cavity is located on the supporting part 331 and cannot fall down from the through hole 211. Then, the carrying portion 331 may be slowly lowered by the lifting mechanism 330, and when the upper surface of the lowermost culture dish in the sorting accommodation chamber moves to a position (preferably, to a position flush with, slightly higher than, or slightly lower than the upper surface of the bottom plate 210), the lowering is stopped, and the culture dish holder 220 is rotated to prevent the culture dish that is not to be taken out from falling down when the carrying portion 331 is lowered again. Then, the holding unit 331 is further lowered to separate the culture dish taken out from the through hole 211 and the culture dish holder 220, the culture dish tray 710 is separated from the dish lid 720 by entering the culture dish opening and closing device 300, and the mixed liquid is added to the dish tray by the sample addition system 400.

After being added the mixed liquid that multiple material constitutes in the culture dish that takes out, culture dish device 300 that opens and shuts makes dish 710 combine with dish lid 720, after the combination, the portion of carrying 331 carries this culture dish and continues the rebound, when the upper surface of this culture dish moved to with the upper surface parallel and level of bottom plate 210, or be less than the position of the upper surface of bottom plate 210 (preferably parallel and level, a little more than or a little less than all can), stop rising, rotate culture dish frame 220 to make a storage hold the chamber and rotate the top that is located through-hole 211 everywhere. The lower surface of the culture dish is then moved to a position flush with the upper surface of the base plate 210 or higher than the upper surface of the base plate 210. Finally, the dish holder 220 is rotated to prevent the dish in the storage chamber from falling after the carrying portion 331 is lowered. This allows the culture dish to be stored in the storage accommodating chamber.

Further, the culture dish holder 220 may include a plurality of rack bars extending in a vertical direction, an upper support plate holder, and a lower support plate holder. The plurality of bars defines a plurality of pockets, for example, one pocket for every four bars. The lower supporting plate frame and the upper supporting plate frame are respectively arranged at two ends of each frame rod.

Preferably, the culture dish storing and sorting device 200 further comprises at least one tray 240 disposed at a lower portion of each of the storage accommodating chambers, each tray 240 being configured to move under the upward movement of the culture dish carried by the tray to allow the culture dish carried by the tray to move upward above it, and to return to an initial position after the culture dish carried by the tray moves upward above it to carry the culture dish thereon. This kind of setting can make the storage step of culture dish simpler, after being added multiple material in the culture dish that takes out, when needing to store this culture dish, can make a storage hold the chamber and rotate to being in the top of through-hole 211 earlier, then make this culture dish be in on elevating system 330's the portion 331 that carries, the portion 331 that carries this culture dish rebound, until the height of the portion 331 that carries is higher than collet 240, collet 240 allows first the portion 331 that carries and culture dish to pass through, then return initial position, then the portion 331 that carries descends, the culture dish can be located collet 240, the whereabouts of the portion 231 that carries can be convenient for to this kind of structure. Further, the tray 240 may also provide clearance between the stack of plates and the base plate 210 to facilitate removal of the plates by a user. Specifically, each shoe 240 includes at least three support posts, each of which has one end rotatably mounted to a lower portion of one of the storage receiving cavities to rotate upward when the culture dish carried by the tray moves upward, thereby allowing the culture dish carried by the tray to move upward thereto, and to rotate downward to return to an original position after the culture dish carried by the tray moves upward thereto, thereby supporting the culture dish with the rod portion thereof.

In some embodiments of the present invention, the station carousel 320 of the culture dish lid opening and closing device 300 is further configured to drive the dish from a dish lid opening and closing station to a mixed liquid adding station, and to drive the dish from the mixed liquid adding station to the dish lid opening and closing station after the mixed liquid is added to the dish. In this case, the station turntable 320 is preferably rotatably mounted to the frame, the second stepped holes 321 on the station turntable 320 are preferably plural, the upper tray 310 is preferably rotated in synchronization with the station turntable 320, and the first stepped holes 311 on the upper tray 310 are also preferably plural.

In some embodiments of the invention, the sample addition system 400 can include a plurality of mixing tubes 410, a first drive device, a plurality of sample storage tubes 420, a sampling device, a plurality of solution storage containers 440, and a solution withdrawal device.

The first drive means are configured to move each mixing tube 410 at least to a first station and a second station, respectively in two positions.

The plurality of sample storage tubes 420 are used to hold a plurality of samples, respectively.

The sampling device may be configured to remove a specimen from one of the specimen storage tubes 420 and deliver the removed specimen to the mixing tube 410 at the first station. The sampling device may also be configured to remove mixed liquor from one of the mixing tubes 410 and then deliver the removed mixed liquor to a dish at a mixed liquor addition station.

The plurality of solution storage containers 440 are respectively used to contain a plurality of solutions.

The solution withdrawing device is configured to withdraw and transfer the solutions in the plurality of solution storage containers 440 into the mixing tube 410 at the second station in a predetermined order.

In this embodiment, the first drive mechanism is further configured to move each mixing tube 410 to the first station to receive one sample at the first station and then to the second station to receive multiple solutions. A plurality of mixing tubes 410 and a first drive mechanism are used to move one mixing tube 410 to a first station and a second station to receive a plurality of solutions and a sample from a sample storage tube 420 to form a mixed solution. Another mixing tube 410 may then be moved to the first and second stations to receive the plurality of samples and another sample in another sample storage tube 420 to form another mixture. The plurality of mixing vials 410 may form a plurality of mixed solutions. In this embodiment, the first drive means are also configured to move a plurality of adjacent mixing tubes 410 in sequence to a first station where the same sample is received, differing in how many of the samples received are not identical, to form a sample gradient; and then moves to a second station to receive the plurality of solutions. The use of multiple mixing cuvettes 410 and a first drive means significantly improves the efficiency of the experiment.

In some embodiments of the present invention, a plurality of sample storage tubes 420 are spaced apart in a circumferential direction. The sampling device may include a sampling syringe, a first rotary lift device 431, and a first plunger pump 432. The first rotary lift device 431 is configured to rotate at least the sampling needle above the mixing tube 410 or above each sample storage tube 420 at the first station and to move the sampling needle up and down. The first plunger pump 432 is in communication with the sampling needle via tubing to draw the sampling needle into the sample storage tube 420 and eject the sample from the sampling needle into the mixing tube 410 at the first station.

When the sample in the sample storage tube 420 needs to be taken out, the sampling needle tube is rotated to the upper side of the sample storage tube 420, and then the sampling needle tube is lowered to extend into the lower side of the solution in the sample storage tube 420, and then the first plunger pump 432 is controlled to work, so that the sample in the sample storage tube 420 enters the sampling needle tube. Then, the sampling needle tube is lifted and rotated to be rotated above the mixing test tube 410 at the first station, then the sampling needle tube is lowered, and finally the sample entering the sampling needle tube is discharged from the sampling needle tube into the mixing test tube 410 at the first station, so that the sample is taken out.

In some embodiments of the present invention, the solution withdrawing means comprises a second rotary elevating means 451, a plurality of liquid withdrawing syringes 452, a second plunger pump 453, and a first flow path switching means. Second rotary lift 451 is configured to rotate each of fluid-extracting needle tubes 452 above one of the mixing tubes 410 at the second station or above one of the solution storage containers 440, and is configured to move each of fluid-extracting needle tubes 452 up and down. Second plunger pump 453 is in communication with each of the fluid-extracting syringes 452 via tubing. First flow switching apparatus is configured to controllably engage one of fluid-withdrawal needle tubes 452 and second plunger pump 453 to controllably cause second plunger pump 453 to cause each fluid-withdrawal needle tube 452 to draw solution from one solution storage container 440 and to cause solution entering fluid-withdrawal needle tube 452 to be expelled from fluid-withdrawal needle tube 452 into mixing tube 410 at the second station.

When multiple solutions need to be taken out, two ways are available: in a first embodiment, multiple fluid extraction needles 452 may be positioned above multiple fluid storage containers 440, and then multiple fluid extraction needles 452 may be lowered into multiple fluid storage containers 440. The first flow path switching means first switches on one of the fluid-extracting needle tubes 452 to the second plunger pump, so that the fluid is sucked into the fluid-extracting needle tube 452. Then, the second channel switching means connects the other fluid-extracting needle tube 452 to the second plunger pump, so that the fluid is sucked into the fluid-extracting needle tube 452. Then, the second flow path switching means connects the third syringe 452 to the plunger pump to draw in the solution from the syringe 452. … …. Until the last syringe 452 is filled with the corresponding solution. When a certain liquid-extracting needle tube 452 is positioned above the mixing tube 410, the first flow-path switching device turns on the liquid-extracting needle tube 452 and the second plunger pump 453 so that the solution in the liquid-extracting needle tube 452 is discharged to the mixing tube 410 positioned at the second station. In the second embodiment, a plurality of liquid extraction needle tubes 452 may be positioned above a plurality of solution storage containers 440, and then the plurality of liquid extraction needle tubes 452 may be lowered into the plurality of solution storage containers 440. The first flow path switching means first connects one of the pipette needle 452 and the second plunger pump to suck the solution into the pipette needle 452. The plurality of liquid-extracting needle tubes 452 are raised and rotated, and the liquid-extracting needle tube 452 that has sucked the solution is rotated to a position above the second-stage mixing tube 410, so that the solution in the liquid-extracting needle tube 452 is discharged to the second-stage mixing tube 410. The plurality of sampling syringes are then rotated so that the plurality of access syringes 452 are positioned above the plurality of solution storage containers 440, and then the plurality of access syringes 452 are lowered into the plurality of solution storage containers 440. The first flow path switching means connects the other fluid-extracting needle tube 452 to the second plunger pump, so that the fluid-extracting needle tube 452 sucks the solution. The plurality of liquid-extracting needle tubes 452 are raised and rotated, and the liquid-extracting needle tube 452 that has sucked the solution is rotated to a position above the second-stage mixing tube 410, so that the solution in the liquid-extracting needle tube 452 is discharged to the second-stage mixing tube 410.… …. Until all of the solution in the solution storage container 440 is removed and placed into the mixing tube 410 at the second station.

In some embodiments of the present invention, the sampling device may further comprise a sample feeding syringe and a second flow path switching device. The sample feeding needle tube is arranged on the first rotary lifting device 431, so as to be driven by the first rotary lifting device 431 to rotate to the position above the mixing test tube 410 at the first station, and to be driven by the first rotary lifting device 431 to perform lifting movement; and the sample feeding needle tube is communicated with the first plunger pump 432 through a pipeline to absorb the mixed liquid from the mixing test tube 410 at the first station. The second flow switching device is configured to controllably communicate the sample syringe with the first plunger pump 432 or communicate the sample syringe with the first plunger pump 432. The first plunger pump is also configured to discharge the mixed liquid entering the sample feeding needle tube into the dish of the culture dish.

After each mixed test tube 410 received a sample at first station, and moved to the second station, and the stand horse returns first station after receiving multiple solution, and the needle tubing that send a sample can carry out reciprocating lifting motion to make the multiple material in the mixed test tube 410 form more even mixed liquid, then carry the mixed liquid in this mixed test tube to the dish of one or more culture dishes in.

In some embodiments of the invention, the sample addition system 400 further comprises a wash tank. The inlet of the second flow path switching device is also in controlled communication with the air compressor and the water supply pump through a valve device. The water supply pump is also used to supply the cleaning solution to the cleaning bath, for example, an inlet of the water supply pump is controllably connected to a water tank or an alcohol container. The first rotary lifter 431 is configured to rotate the sampling needle and the sample feeding needle above the wash tank and then descend into the wash tank. When the cleaning is required, the first rotary lifting device 431 can make the sampling needle tube and the sample feeding needle tube be positioned above the cleaning tank, and the water supply pump can firstly inject water into the cleaning tank and the needle tube to clean the inside or the outside of the needle tube. Then, the water supply pump injects alcohol into the cleaning tank and the needle tube, and finally, the air compressor can inject air into the needle tube to clean the needle tube.

In some embodiments of the present invention, a plurality of mixing vials 410 are spaced apart in a circumferential direction. The first driving means may include: a rotating cylinder to which the plurality of mixing test tubes 410 are mounted; a driving motor configured to drive the rotating cylinder to perform a rotational motion.

In some embodiments of the present invention, the Ames tester may further comprise a shaking device 500 for further homogenizing the mixture in the dish. Specifically, the shaking device 500 includes a base, a driving wheel, a driven wheel, a shaking frame, a lifting device and a carrying tray. The base is mounted to a base 100. The driving wheel and the driven wheel are both rotatably arranged on the base. The shaking frame is respectively and rotatably arranged on the driving wheel and the driven wheel at two parts.

In particular, in this embodiment, the base, the driving wheel, the driven wheel and the pan carriage together form a crank-rocker mechanism or a parallelogram mechanism. Further, the cradle includes a base plate and a side plate having an upper end disposed on one side of the base plate. The base plate is rotatably mounted on the driving wheel and the driven wheel at two positions on the lower surface of the base plate respectively. For example, the substrate has rotary holes at two positions on the lower surface thereof; the shaking device 500 further comprises two rollers respectively mounted on the upper surfaces of the driving wheel and the driven wheel and respectively located in the two rotation holes. The lifting device is arranged on the side plate. The object carrying plate is used for placing a culture dish and is arranged on the lifting device so as to be driven by the lifting device to move up and down. The carrier plate is preferably a suction cup. The lifting device can at least enable the dish plate 710 of the culture dish to be separated from the second stepped hole 321, so that the second stepped hole 321 can be prevented from obstructing the movement of the dish plate in the rotating process. In order to better and more accurately move the carrier tray to a predetermined position for carrying a dish, the shake-up device 500 further includes a position detection device configured to detect whether the shake-up rack is at or back to the initial position. For example, the position detecting device includes a signal sheet mounted at an edge of the substrate and a sensor mounted on the base.

In some embodiments of the present invention, the Ames tester may further comprise a marking device 600 configured to place indicia on the outer surface of the sidewall or the outer surface of the bottom wall of the dish tray at the marking station to prevent confusion caused by placing indicia on the dish lid. Specifically, the coding device 600 may include a code base, at least one slider, at least one coding pen, and a cylinder. The dock is mounted to the base 100. At least one slider is slidably mounted on the nest. Each marking pen is fixed on one sliding block to move telescopically along with the sliding block, so that marks are arranged on the outer surface of the side wall of the dish at the marking station. The cylinder is configured to controllably cause telescopic movement of one coding pen with each slider mounted on the slider. Further, the dock includes an inclined plate and a support plate disposed below the inclined plate. At least one slider is mounted on the inclined plate for telescopic movement in a direction parallel to the inclined plate. The pen point of each coding pen faces to the lower direction in an inclined mode, so that the marks can be better arranged on the side wall of the dish.

The foregoing is merely a preferred embodiment of this invention and it should be understood that any modifications and alterations herein can be made by those skilled in the art without departing from the principles of this invention.

Claims

1. A culture dish device of opening and closing cover for ames tester, which is characterized in that

A support;

the station turntable is fixedly or rotatably arranged on the bracket and is positioned below the upper disc, at least one second step hole is formed in the station turntable, and the diameter of the station turntable is larger than that of the upper disc; and

a lifting mechanism having a carrying part and arranged in a manner of

when the first step hole for carrying the dish cover and the second step hole for carrying the dish plate are positioned at the same axial position, the carrying part moves upwards from the lower part of the station turntable so that the carrying part sequentially passes through the second step hole and the first step hole, and in the moving process of the carrying part, the dish plate of the culture dish moves upwards to be matched with the dish cover of the culture dish, so that the dish cover of the culture dish is arranged on the dish plate;

and the rotating shaft of the station turntable and the rotating shaft of the upper disc are arranged in parallel at intervals.

2. The culture dish opening and closing device according to claim 1,

the station turntable is rotatably mounted to the support and is configured to transport a dish tray located in the second stepped bore to each of the predetermined stations.

3. The culture dish opening and closing device according to claim 1,

the number of the first stepped holes is multiple, and the number of the first stepped holes is equal to that of the second stepped holes;

the station carousel with the hanging wall rotationally install in the support, just the station carousel with the hanging wall rotates in step.

4. The cover opening and closing device for culture dish of claim 3, further comprising

And the rotating device is arranged below the station turntable and is configured to drive the station turntable to rotate.

5. The cover opening and closing device for culture dish of claim 3, further comprising

A linkage configured to cause the station carousel and the upper tray to rotate synchronously.

6. The culture dish opening and closing device according to claim 1,

the lifting mechanism further comprises a linear sliding table so as to drive the supporting and carrying part to move up and down.

7. A culture dish device according to claim 1, it is characterized in that the preparation method is characterized in that,

the supporting part is a bracket or a tray.

8. An Ames tester, characterized by comprising the culture dish device of opening and closing of any one of claims 1 to 7, the culture dish device of opening and closing of closing is configured to separate the dish lid and the dish of culture dish, wait to add mixed liquid in the dish, will the dish lid install in the dish.

9. The Ames tester as recited in claim 8 wherein the station carousel of the culture dish lid opening and closing device is configured to drive the dish from a dish lid opening and closing station to a mixed liquor addition station and to drive the dish from the mixed liquor addition station to the dish lid opening and closing station after the mixed liquor is added to the dish.