CN107854994B - The environmentally protective self-degradation bioactive enzyme of organic pollutant, preparation method and application can be swallowed - Google Patents
The environmentally protective self-degradation bioactive enzyme of organic pollutant, preparation method and application can be swallowed Download PDFInfo
- Publication number
- CN107854994B CN107854994B CN201711100030.XA CN201711100030A CN107854994B CN 107854994 B CN107854994 B CN 107854994B CN 201711100030 A CN201711100030 A CN 201711100030A CN 107854994 B CN107854994 B CN 107854994B
- Authority
- CN
- China
- Prior art keywords
- weight
- parts
- added
- cellulase
- vegetable protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
- B01D53/84—Biological processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/38—Removing components of undefined structure
- B01D53/44—Organic components
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
- B01D53/86—Catalytic processes
- B01D53/8678—Removing components of undefined structure
- B01D53/8687—Organic components
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2251/00—Reactants
- B01D2251/95—Specific microorganisms
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2255/00—Catalysts
- B01D2255/20—Metals or compounds thereof
- B01D2255/207—Transition metals
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2255/00—Catalysts
- B01D2255/80—Type of catalytic reaction
- B01D2255/802—Photocatalytic
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2257/00—Components to be removed
- B01D2257/70—Organic compounds not provided for in groups B01D2257/00 - B01D2257/602
- B01D2257/708—Volatile organic compounds V.O.C.'s
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2258/00—Sources of waste gases
- B01D2258/06—Polluted air
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
The present invention relates to the environmentally protective self-degradation bioactive enzymes that can swallow organic pollutant, bioactive enzyme is made of the deionized water of the vegetable protein extracting solution of 5-15 parts by weight, the polyvinyl alcohol of 1-5 weight, the polyphenol of 1-3 parts by weight and 100 parts by weight, and tannase is added in the vegetable protein extracting solution during the extraction process.Bioactive enzyme of the invention can effectively remove organic pollutant.
Description
Technical field
The present invention relates to the environmentally protective self-degradation bioactive enzymes that can swallow organic pollutant, and the invention further relates to can gulp down
The preparation method for biting the environmentally protective self-degradation bioactive enzyme of organic pollutant, moreover, it relates to can swallow organic
Application of the environmentally protective self-degradation bioactive enzyme of pollutant in air purification field.
Background technique
The pollution of volatile organic matter indoors has the characteristics that multipath, concealment, characteristic of concentration.It distributes approach
Building materials, ornament materials and articles for daily use etc., simultaneously because the slow release characteristic of volatile organic matter is toward being not easy to be examined by people
Feel, and itself will not be decomposed quickly, the extent of injury that this allows for volatile organic matter is more tight compared with other polluters
Weight.
Interior is evaporated into after surface treatment containing volatile organic matters such as a large amount of formaldehyde, how effectively to remove these
Volatile organic matter is a great problem of this field.
Summary of the invention
In order to solve the above-mentioned technical problems, the present invention provides the environmentally protective self-degradation biologies that can swallow organic pollutant
Organized enzyme, the bioactive enzyme is by the vegetable protein extracting solution of 5-15 parts by weight, the polyvinyl alcohol of 1-5 weight, 1-3 parts by weight
Polyphenol and 100 parts by weight deionized water composition, tannase is added in the vegetable protein extracting solution during the extraction process.
The degree of polymerization of the polyvinyl alcohol is 17-24, alcoholysis degree 80-90%.
The vegetable protein extracting solution is prepared by following preparation method:
With sansevieria trifasciata prain leaf, Siraitia grosvenorii, purslane, galangal, large grass, roseleaf, tealeaves etc. for raw material, raw material is clear
It is placed on after washing in mortar and uses liquid nitrogen grinding, stood at room temperature to room temperature, juice and filter residue is obtained by filtration, it is molten that cellulase is added
The volume ratio of liquid, cellulase solution and juice is 1:1-3, and the mass ratio of cellulase solution cellulase is 1-5%, is surpassed
Sonication, 100 ~ 250W of power, 20 ~ 40kHz of frequency, after continuing 1-3 hours；The pectase of juice quality 1-5%, ultrasound is added
After processing 1-3 hours, extracting solution is obtained；PH value is adjusted after enzyme deactivation to 3-5, then under the centrifugal action of 4000 ~ 5000r/min
15 ~ 20min is persistently handled, removal supernatant liquor obtains vegetable protein extract；Vegetable protein extract is dissolved in 2-10 times of matter
In the deionized water of amount, the tannase of vegetable protein extract quality 0.01-0.5% is added, is ultrasonically treated 10-30 minutes, enzyme deactivation
After obtain vegetable protein extracting solution.
The polyphenol is plant polyphenol extracting solution.
The plant polyphenol is prepared by the following method to obtain:
Using sansevieria trifasciata prain leaf, Siraitia grosvenorii, purslane, galangal, large grass, roseleaf, tealeaves mixture as raw material,
It is placed in mortar after raw material cleaning and uses liquid nitrogen grinding, stood at room temperature to room temperature, juice and filter residue is obtained by filtration, cellulose is added
The volume ratio of enzyme solutions, cellulase solution and juice is 1:1-3, and the mass ratio of cellulase solution cellulase is 1-
5%, ultrasonic treatment, 100 ~ 250W of power, 20 ~ 40kHz of frequency, after continuing 1-3 hours；The pectase of juice quality 1-5% is added,
Ultrasonic treatment obtained extracting solution after 1-3 hours；PH value is adjusted after enzyme deactivation to 3-5, is then made in the centrifugation of 4000 ~ 5000r/min
15 ~ 20min is persistently handled with lower, takes supernatant liquor, the ethyl alcohol of supernatant liquor volume 20%-60% is added, ultrasonic extraction is evaporated dense
After being reduced to the 30% of original volume, ethyl acetate extraction is added, takes ethyl acetate layer, is dried to obtain plant polyphenol under nitrogen atmosphere.
The bioactive enzyme further includes the modified Nano niobium oxide of 1-5 parts by weight, and the modified Nano niobium oxide passes through
Following steps are prepared:
At room temperature, the columbium pentachloride of 3-10 parts by weight is dissolved in 100 parts by weight dehydrated alcohols, obtains columbium pentachloride second
Alcoholic solution stands 5-30 minutes；
By the pucherite of 5-15 parts by weight, the blocked isocyanate of 0.01-0.1 parts by weight is added molten to columbium pentachloride ethyl alcohol
Liquid closes uniformly, obtains the first mixed liquor；
It is kept stirring the first mixed liquor, 100 parts by weight of ethanol aqueous solutions are instilled first with the speed of 3-10ml/min and are mixed
It closes in liquid；
The n,N-Dimethylformamide of 100 parts by weight is added, is kept stirring the first mixed liquor 30 minutes, is stored at room temperature to obtain
Second mixed liquor；
After being soaked in water 6-24 hours under the second mixed liquor and 30-45 degrees Celsius, it is warming up to 40-45 degrees Celsius of drying
After 12-48 hours, it is warming up to blocked isocyanate envelope temperature and is kept for 3-10 hours, be warming up to 100-160 degrees Celsius of drying extremely
Constant weight obtains dried object, and the deblocking temperature of the blocked isocyanate is greater than 50 degrees Celsius, less than 100 degrees Celsius；
By dried object in Muffle furnace, in 400-500 temperature lower calcination, nano modification niobium oxide is obtained.
The blocked isocyanate is that sodium hydrogensulfite closes HDI.
The present invention handles leaves of plants and petal by special processing method, obtains with the first in absorption air very well
The spray product of aldehyde.Since vegetable protein is easy in conjunction with other molecules in plant extracts during precipitating, and it is difficult
With separation, the present invention is found through experiments that, the tannic acid in leaves of plants and petal is easy in conjunction with vegetable protein, since tannic acid has
Biggish molecular structure and more active group, in conjunction with protein after will continue to react, change protein original three
Structure is tieed up, so that protein loses corresponding formaldehyde treated activity.The generation that tannic acid inhibits this phenomenon is added.In addition, this
Invention is by introducing polyphenolic substance, so that having light-catalysed niobium oxide not make protein during long-term storage
Lose the activity of formaldehyde treated.Use sodium hydrogensulfite blocked isocyanate and pucherite as doping vario-property compound, must make
The standby obtained long-acting enzyme of spectrum has the efficient ability of the decomposing organic matter under dim light.
The above-mentioned of the application and other features, aspects and advantages are more readily understood with reference to following detailed description.
Detailed description of the invention
Fig. 1 is that the SEM of nano modification niobium oxide photocatalyst schemes.
Specific embodiment
Hereinafter, the present invention is explained in more detail by embodiment, it should be appreciated that these embodiments are only
Illustrate and not restrictive.If raw materials used to be all commercially available without other explanations.
Referring to several example the present invention is described in detail.
Polyvinyl alcohol 2488, Shanxi Sheng Tai, trade mark 24-88.
Nano modification niobium oxide light photocatalyst
The preparation of pucherite
The bismuth nitrate of the sodium vanadate of 0.0015mol and 0.006mol is dissolved separately in deionized water, sodium vanadate is molten
Liquid instills in bismuth nitrate solution dropwise, after stirring 30 minutes, after standing 5 hours, is put into thermostatic drying chamber, with 160 degrees Celsius
After lower reaction 12 hours, after being cooled to room temperature, continue the hydrogen peroxide for being kept stirring and being added 0.01mol, filter, washing, drying,
Obtain pucherite powder.
At room temperature, the columbium pentachloride of 8 parts by weight is dissolved in 100 parts by weight dehydrated alcohols, obtains columbium pentachloride ethyl alcohol
Solution stands 15 minutes；The sodium hydrogensulfite blocked TDI of the pucherite of 15 parts by weight, 0.08 parts by weight is added to phosphoric
Niobium ethanol solution closes uniformly, obtains the first mixed liquor；Be kept stirring the first mixed liquor, by 100 parts by weight of ethanol aqueous solutions with
The speed of 4ml/min instills in the first mixed liquor；The n,N-Dimethylformamide of 30 parts by weight is added, is kept stirring the first mixing
It liquid 30 minutes, is stored at room temperature to obtain the second mixed liquor；After being soaked in water 12 hours under the second mixed liquor and 40 degrees Celsius, heating
After being dried 24 hours to 45 degrees Celsius, it is warming up to 60 degrees Celsius and is kept for 4 hours, be warming up to 150 degrees Celsius of dryings to constant weight, obtain
To dried object；By dried object in Muffle furnace, in 450 temperature lower calcinations, nano modification niobium oxide photocatalyst is obtained.
SEM figure is shown in Fig. 1.
Embodiment 1
The mass ratio of large grass and Siraitia grosvenorii is 1:1, is placed in mortar after cleaning with liquid nitrogen grinding, stand at room temperature to
Room temperature, is obtained by filtration juice and filter residue, is added cellulase solution, and the volume ratio of cellulase solution and juice is 1:2, fiber
The mass ratio of plain enzyme solutions cellulase is 3%, ultrasonic treatment, power 250W, frequency 30kHz, after continuing 2 hours；Juice is added
The pectase of liquid quality 3%, ultrasonic treatment obtained extracting solution after 2 hours；Then existed using salt acid for adjusting pH value to 4 after enzyme deactivation
20min is persistently handled under the centrifugal action of 5000r/min, removal supernatant liquor obtains vegetable protein extract；By vegetable protein
Extract is dissolved in the deionized water of 8 times of quality, and the tannase of vegetable protein extract quality 0.1% is added, and is ultrasonically treated 20 points
Clock obtains vegetable protein extracting solution after enzyme deactivation.
By the vegetable protein extracting solution of 10 parts by weight, the polyvinyl alcohol of 2 weight, the tea polyphenols of 2 parts by weight and 100 weight
The deionized water of part is uniformly mixed, and obtains bioactive enzyme.
Embodiment 2
Roseleaf and the mass ratio of purslane are 1:5, are placed in mortar after cleaning with liquid nitrogen grinding, stand at room temperature to
Room temperature, is obtained by filtration juice and filter residue, is added cellulase solution, and the volume ratio of cellulase solution and juice is 1:2, fiber
The mass ratio of plain enzyme solutions cellulase is 3%, ultrasonic treatment, power 250W, frequency 30kHz, after continuing 2 hours；Juice is added
The pectase of liquid quality 3%, ultrasonic treatment obtained extracting solution after 2 hours；Then existed using salt acid for adjusting pH value to 4 after enzyme deactivation
20min is persistently handled under the centrifugal action of 5000r/min, removal supernatant liquor obtains vegetable protein extract；By vegetable protein
Extract is dissolved in the deionized water of 8 times of quality, and the tannase of vegetable protein extract quality 0.1% is added, and is ultrasonically treated 20 points
Clock obtains vegetable protein extracting solution after enzyme deactivation.
By the vegetable protein extracting solution of 13 parts by weight, the polyvinyl alcohol of 1 weight, the tea polyphenols of 1 parts by weight and 100 weight
The deionized water of part is uniformly mixed, and obtains bioactive enzyme.
Embodiment 3
The mass ratio of galangal and green tea is 1:0.3, is placed in mortar after cleaning and uses liquid nitrogen grinding, stood at room temperature
To room temperature, juice and filter residue is obtained by filtration, is added cellulase solution, the volume ratio of cellulase solution and juice is 1:2, fine
The mass ratio for tieing up plain enzyme solutions cellulase is 3%, ultrasonic treatment, power 250W, frequency 30kHz, after continuing 2 hours；It is added
The pectase of juice quality 3%, ultrasonic treatment obtained extracting solution after 2 hours；Using salt acid for adjusting pH value to 4 after enzyme deactivation, then
20min is persistently handled under the centrifugal action of 5000r/min, removal supernatant liquor obtains vegetable protein extract；By plant egg
White extract is dissolved in the deionized water of 8 times of quality, and the tannase of vegetable protein extract quality 0.1%, ultrasonic treatment 20 is added
Minute, vegetable protein extracting solution is obtained after enzyme deactivation.
By the vegetable protein extracting solution of 8 parts by weight, the polyvinyl alcohol of 3 weight, 2 parts by weight plant polyphenol extracting solution (on
The supernatant liquor stated is obtained by extraction, polyphenol content 45%) and 100 parts by weight deionized water be uniformly mixed, obtain bioactivity
Enzyme.
Embodiment 4
It is same as Example 1, the modified Nano niobium oxide of 2 parts by weight is added in bioactive enzyme.
Comparative example 1
It is same as Example 1, P25 type nano-titanium oxide is added.
Comparative example 2
The mass ratio of roseleaf and green tea is 1:1, is placed in mortar after cleaning and uses liquid nitrogen grinding, stood at room temperature
To room temperature, juice and filter residue is obtained by filtration, is added cellulase solution, the volume ratio of cellulase solution and juice is 1:2, fine
The mass ratio for tieing up plain enzyme solutions cellulase is 3%, ultrasonic treatment, power 250W, frequency 30kHz, after continuing 2 hours；It is added
The pectase of juice quality 3%, ultrasonic treatment is after 2 hours, the tannase of addition vegetable protein extract quality 0.1%, at ultrasound
Reason 20 minutes, obtains extracting solution；Using salt acid for adjusting pH value to 4 after enzyme deactivation, then continue under the centrifugal action of 5000r/min
20min is handled, removal supernatant liquor obtains vegetable protein extract；Vegetable protein extract is dissolved in the deionization of 8 times of quality
In water, it is ultrasonically treated 20 minutes, obtains vegetable protein extracting solution.
Comparative example 3
It is same as Example 1, it is added without 0.1% tannase.
Comparative example 4
The nano oxidized titanium solution of P25 type that mass fraction is 10% is substituted into bioactive enzyme.
Evaluation method:
The environment for simulating volatile organic matter sprays into formaldehyde and toluene, obtained VOC concentration in the 20L container of sealing
About 3.4 μ g/m L.One spray gun is set in container, by the aforementioned bioactive enzyme spray point being prepared 5 times of 5ml every
Spray in 10 minutes is primary, until this container is put in incubator and is tested in confined space, temperature is room temperature, day in incubator
Light lamp intensity is 200lux, tests VOC content using GT-1000-VOC pump suction type VOC detector after 8 hours, calculates VOC removal
Rate.
It can be seen that bioactive enzyme of the invention after atomization by above-mentioned experiment, suspending can have in air
Volatile organic matter in effect removal air.
The foregoing is only a preferred embodiment of the present invention, is not intended to limit the scope of the present invention.It is all
The equivalent changes and modifications that content is done according to the present invention are encompassed by the scope of the patents of the invention.
Claims (5)
1. the environmentally protective self-degradation bioactive enzyme of organic pollutant can be swallowed, which is characterized in that the bioactive enzyme by
The vegetable protein extracting solution of 5-15 parts by weight, the polyvinyl alcohol of 1-5 weight, the polyphenol of 1-3 parts by weight and 100 parts by weight are gone
Tannase is added in ionized water composition, the vegetable protein extracting solution during the extraction process；
The bioactive enzyme further includes the modified Nano niobium oxide of 1-5 parts by weight, and the modified Nano niobium oxide passes through following
Step is prepared:
At room temperature, the columbium pentachloride of 3-10 parts by weight is dissolved in 100 parts by weight dehydrated alcohols, it is molten obtains columbium pentachloride ethyl alcohol
Liquid stands 5-30 minutes；
By the pucherite of 5-15 parts by weight, the blocked isocyanate of 0.01-0.1 parts by weight is added to columbium pentachloride ethanol solution,
It is uniformly mixed, obtains the first mixed liquor；
It is kept stirring the first mixed liquor, 100 parts by weight of ethanol aqueous solutions are instilled into the first mixed liquor with the speed of 3-10ml/min
In；
The n,N-Dimethylformamide of 100 parts by weight is added, is kept stirring the first mixed liquor 30 minutes, is stored at room temperature to obtain second
Mixed liquor；
After second mixed liquor is soaked in water 6-24 hours under 30-45 degrees Celsius, it is warming up to 40-45 degrees Celsius of dry 12-48
It after hour, is warming up to blocked isocyanate envelope and temperature and is kept for 3-10 hours, be warming up to 100-160 degrees Celsius of drying to constant weight,
Dried object is obtained, the deblocking temperature of the blocked isocyanate is greater than 50 degrees Celsius, less than 100 degrees Celsius；
By dried object in Muffle furnace, in 400-500 degrees Celsius of temperature lower calcination, nano modification niobium oxide is obtained；
The blocked isocyanate is that sodium hydrogensulfite closes hexamethylene diisocyanate.
2. the environmentally protective self-degradation bioactive enzyme according to claim 1 for swallowing organic pollutant, feature exist
In the degree of polymerization of the polyvinyl alcohol is 17-24, alcoholysis degree 80-90%.
3. the environmentally protective self-degradation bioactive enzyme according to claim 1 for swallowing organic pollutant, feature exist
In the vegetable protein extracting solution is prepared by following preparation method:
Using sansevieria trifasciata prain leaf, Siraitia grosvenorii, purslane, galangal, large grass, roseleaf, tealeaves as raw material, it is placed on and grinds after cleaning
Liquid nitrogen grinding is used in alms bowl, is stood at room temperature to room temperature, juice and filter residue is obtained by filtration, and cellulase solution, cellulase is added
The volume ratio of solution and juice is 1:1-3, and the mass ratio of cellulase solution cellulase is 1-5%, ultrasonic treatment, power
100 ~ 250W, 20 ~ 40kHz of frequency, after continuing 1-3 hours；The pectase of juice quality 1-5% is added, is ultrasonically treated 1-3 hours
Afterwards, extracting solution is obtained；PH value is adjusted after enzyme deactivation to 3-5, then persistently handle 15 under the centrifugal action of 4000 ~ 5000r/min ~
20min, removal supernatant liquor obtain vegetable protein extract；Vegetable protein extract is dissolved in the deionized water of 2-10 times of quality
In, the tannase of vegetable protein extract quality 0.01-0.5% is added, is ultrasonically treated 10-30 minutes, plant egg is obtained after enzyme deactivation
White extracting solution.
4. the environmentally protective self-degradation bioactive enzyme according to claim 1 for swallowing organic pollutant, feature exist
In the polyphenol is plant polyphenol extracting solution.
5. the environmentally protective self-degradation bioactive enzyme according to claim 1 for swallowing organic pollutant, feature exist
In the plant polyphenol is prepared by the following method to obtain:
Using sansevieria trifasciata prain leaf, Siraitia grosvenorii, purslane, galangal, large grass, roseleaf, tealeaves as raw material, it is placed on and grinds after cleaning
Liquid nitrogen grinding is used in alms bowl, is stood at room temperature to room temperature, juice and filter residue is obtained by filtration, and cellulase solution, cellulase is added
The volume ratio of solution and juice is 1:1-3, and the mass ratio of cellulase solution cellulase is 1-5%, ultrasonic treatment, power
100 ~ 250W, 20 ~ 40kHz of frequency, after continuing 1-3 hours；The pectase of juice quality 1-5% is added, is ultrasonically treated 1-3 hours
Afterwards, extracting solution is obtained；PH value is adjusted after enzyme deactivation to 3-5, then persistently handle 15 under the centrifugal action of 4000 ~ 5000r/min ~
20min takes supernatant liquor, and the ethyl alcohol of supernatant liquor volume 20%-60% is added, and ultrasonic extraction is concentrated by evaporation to the 30% of original volume
Afterwards, ethyl acetate extraction is added, takes ethyl acetate layer, is dried to obtain plant polyphenol under nitrogen atmosphere.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711100030.XA CN107854994B (en) | 2017-11-10 | 2017-11-10 | The environmentally protective self-degradation bioactive enzyme of organic pollutant, preparation method and application can be swallowed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711100030.XA CN107854994B (en) | 2017-11-10 | 2017-11-10 | The environmentally protective self-degradation bioactive enzyme of organic pollutant, preparation method and application can be swallowed |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107854994A CN107854994A (en) | 2018-03-30 |
| CN107854994B true CN107854994B (en) | 2019-11-19 |
Family
ID=61700082
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711100030.XA Active CN107854994B (en) | 2017-11-10 | 2017-11-10 | The environmentally protective self-degradation bioactive enzyme of organic pollutant, preparation method and application can be swallowed |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107854994B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102638999A (en) * | 2010-03-05 | 2012-08-15 | 长谷川香料株式会社 | Method for producing tea extract |
| CN106422755A (en) * | 2016-10-26 | 2017-02-22 | 江阴昊松格氏生物技术有限公司 | Efficient deodorant with pine needle, osmanthus fragrans and biological active enzyme |
| CN107175099A (en) * | 2017-07-21 | 2017-09-19 | 江苏师范大学 | A kind of V ion dopings BiNb5O14Photochemical catalyst and its preparation method and application |
| CN107278220A (en) * | 2015-02-26 | 2017-10-20 | 乐金华奥斯有限公司 | The photocatalyst coated composition of visible light activity and air cleaning filter |
-
2017
- 2017-11-10 CN CN201711100030.XA patent/CN107854994B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102638999A (en) * | 2010-03-05 | 2012-08-15 | 长谷川香料株式会社 | Method for producing tea extract |
| CN107278220A (en) * | 2015-02-26 | 2017-10-20 | 乐金华奥斯有限公司 | The photocatalyst coated composition of visible light activity and air cleaning filter |
| CN106422755A (en) * | 2016-10-26 | 2017-02-22 | 江阴昊松格氏生物技术有限公司 | Efficient deodorant with pine needle, osmanthus fragrans and biological active enzyme |
| CN107175099A (en) * | 2017-07-21 | 2017-09-19 | 江苏师范大学 | A kind of V ion dopings BiNb5O14Photochemical catalyst and its preparation method and application |
Non-Patent Citations (2)
| Title |
|---|
| 室内甲醛降解方法研究进展;宋文芳，李诚，秦志伟;《科技创新与生产力》;20130731;全文 * |
| 茶叶中多酚氧化酶提取工艺及酶学性质的研究;罗琛;《集美大学工程硕士专业学位论文》;20130415;第16-18页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107854994A (en) | 2018-03-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104286247A (en) | Preparation method of detoxified gingko tea | |
| CN101328194A (en) | Persimmon powder and production process thereof | |
| CN106135315A (en) | A kind of natural botanical insecticide | |
| CN107468738A (en) | A kind of extracting method of chrysanthemum total flavone | |
| CN109486604A (en) | A kind of preparation method of Stauntonia latifolia fruit wine | |
| CN107114643B (en) | Red date health concentrated juice and preparation method thereof | |
| JP2008500023A (en) | Aloe powder, aloe juice and method for producing the same | |
| CN110484043A (en) | A kind of safe and efficient plant anion spray | |
| CN107854994B (en) | The environmentally protective self-degradation bioactive enzyme of organic pollutant, preparation method and application can be swallowed | |
| KR20130067004A (en) | The granules comprising charcoal and process for preparing the same | |
| KR102109811B1 (en) | Method for preparing kaffir lime extract and composition comprising kaffir lime extract | |
| KR101040198B1 (en) | the way of abstraction of chlorophyll | |
| CN106955583A (en) | The preparation method of biology enzyme/plant extraction liquid degradation of formaldehyde composite | |
| CN105104920B (en) | A kind of method of hydrogen peroxide and Isoprothiolane in ethyl alcohol removing rice protein under neutrallty condition | |
| CN104310838B (en) | Preparation method and application of building material additive | |
| KR101223746B1 (en) | Mixed Tea Using Leaf, Root Bark and Fruit of Mulberry Tree | |
| CN105968227A (en) | Process of extracting pectin from snake gourd peel | |
| CN113278471A (en) | Shell powder microcapsule for cleaning and sterilizing fruits and vegetables and preparation method thereof | |
| CN112387092A (en) | Formaldehyde scavenger composed of pure natural plant extracts and preparation method thereof | |
| CN105831338A (en) | Production method of sedum aizoon L. tea | |
| CN101258871A (en) | Method for preparing natural nontoxic health care type film type fruit-vegetable antistaling agent | |
| KR101497103B1 (en) | Antioxidants and antibacterial agents from bamboo leaves extraxts | |
| CN110405225A (en) | The preparation method of one Plant Extracts nano materials | |
| JP2006008645A (en) | Method for producing extract of inonotus obliquus | |
| KR102674805B1 (en) | Manufacturing method of Pink Salt using Lactic Acid Bacteria |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: The invention relates to a green environmental protection self degradable bioactive enzyme capable of phagocytizing organic pollutants, a preparation method and application thereof Effective date of registration: 20210513 Granted publication date: 20191119 Pledgee: Hangzhou joint rural commercial bank Limited by Share Ltd. three pier sub branch Pledgor: ZHEJIANG YUWEN ENVIRONMENTAL PROTECTION TECHNOLOGY Co.,Ltd. Registration number: Y2021980003572 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20220726 Granted publication date: 20191119 Pledgee: Hangzhou joint rural commercial bank Limited by Share Ltd. three pier sub branch Pledgor: ZHEJIANG YUWEN ENVIRONMENTAL PROTECTION TECHNOLOGY CO.,LTD. Registration number: Y2021980003572 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right |